Six4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Six4
Entrez Gene ID	40297
Full Name	CG3871 gene product from transcript CG3871-RA
Synonyms	CG3871,D-Six4,D-six4,DSix4,Dmel\CG3871,Dsix4,six4,six4/5
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 77E6-77E6

mRNA and Protein(s)

mRNA	Protein	Name
NM_140999.3	NP_649256.1	Six4, isoform A
NM_168872.1	NP_730566.1	Six4, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011067	XP_001237244.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011065	XP_309580.4
Drosophila melanogaster (fruit fly)	Six4	NP_649256.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Six4 and Gro promote differentiation towards the polar cell fate by promoting Notch pathway activity.
Title: Phosphorylated Groucho delays differentiation in the follicle stem cell lineage by providing a molecular memory of EGFR signaling in the niche.

Six4 is a key regulator of somatic cell function during gonadogenesis
Title: Live imaging of Drosophila gonad formation reveals roles for Six4 in regulating germline and somatic cell migration.

D-six4 is a key mesodermal patterning mediator that regulates a variety of cell-type-specific factors and hence plays an equivalent role to tin.
Title: D-six4 plays a key role in patterning cell identities deriving from the Drosophila mesoderm.

The following Six4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Six4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa21166	NM_168872.1 Latest version!	Drosophila melanogaster Six4, transcript variant B (Six4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa21166	NM_140999.3 Latest version!	Drosophila melanogaster Six4, transcript variant A (Six4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Six4 ( NM_140999.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649256.1 Drosophila melanogaster Six4, transcript variant A (Six4), mRNA.

Price & Availability↑

CloneID	OFa21166
Clone ID Related Accession (Same CDS sequence)	NM_140999.3 , NM_168872.1
Accession Version	NM_140999.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Six4, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140999.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGACA AGAATTTGGA CGGCAACAAT CTGTCGGTCT CCATCGGTGG GGATTTGGAT 
TCGACGAGTT CCGGCGGCAC ATCCTCGGAT CACTCGGCTG TCCATCAGGA CAATCTCAGC 
TCGCCGATGG CCTACGGATC CCTCTTCCTC CCAAATGCTG GTTATCGTGG CAACCTGTCA 
TGCAAAACGG TCCTGCAGCT GGACAAGTTC GCACCCTACG AGGGTGTGGA GAAGGATCAC 
CTGCTGGAGC GGCGGTTTCA GGACATCACC AATGACTACG ACAAGTCGCC GCCGCCCACC 
GCCTCGACCA CGCCCACGCA CTATCCCAGC CTGAACAGCA TAATATTTGA GAATGGCAGC 
AGTGGCAACC TGGGCGATCT CAACGGGAAC ACGAAGACGG ATCTATGCGC CGGCCTGCAG 
AGAAGTGGCG GCGGATTGGG GGGCAATGCC GGCAGTGGTG GCCACCTCAT ATCCAATCTC 
ACAGCCGCCC ACAACATGTC CGCCGTGAGC AGCTTTCCTA TCGATGCCAA GATGCTGCAG 
TTCTCCACGG ATCAGATCCA GTGCATGTGC GAGGCACTGC AGCAGAAGGG CGACATCGAG 
AAGCTGACCA CATTCCTCTG CAGCCTGCCG CCCAGCGAGT TCTTCAAGAC CAACGAGAGT 
GTCCTGCGGG CCCGCGCCAT GGTGGCCTAC AATCTGGGCC AGTTCCACGA GCTGTACAAC 
CTGCTGGAGA CGCACTGCTT TTCGATCAAG TACCACGTGG ACCTGCAGAA CCTCTGGTTC 
AAGGCCCACT ACAAGGAGGC CGAGAAGGTG CGTGGCCGAC CACTGGGCGC CGTGGACAAG 
TACCGTCTGC GCAAGAAGTA TCCCCTGCCC AAGACGATCT GGGATGGCGA GGAGACGGTC 
TACTGCTTCA AGGAGAAGTC GCGCAACGCT CTCAAGGACT GCTACCTGAC CAACCGATAT 
CCCACCCCCG ACGAGAAGAA GACGCTGGCC AAGAAGACCG GGCTCACGCT CACCCAGGTC 
TCCAACTGGT TCAAGAATCG TCGACAGCGG GATCGCACGC CCCAGCAAAG ACCGGACATC 
ATGTCGGTGC TGCCCGTGGG CCAACTGGAT GGCAATGGAT TCCCGCGCAT GTTCAATGCT 
CCAAGCTATT ATCCCGAAAC GATTTTCAAT GGGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649256.1
CDS	491..1669
Translation	MFDKNLDGNNLSVSIGGDLDSTSSGGTSSDHSAVHQDNLSSPMAYGSLFLPNAGYRGNLSCKTVLQLDKFAPYEGVEKDHLLERRFQDITNDYDKSPPPTASTTPTHYPSLNSIIFENGSSGNLGDLNGNTKTDLCAGLQRSGGGLGGNAGSGGHLISNLTAAHNMSAVSSFPIDAKMLQFSTDQIQCMCEALQQKGDIEKLTTFLCSLPPSEFFKTNESVLRARAMVAYNLGQFHELYNLLETHCFSIKYHVDLQNLWFKAHYKEAEKVRGRPLGAVDKYRLRKKYPLPKTIWDGEETVYCFKEKSRNALKDCYLTNRYPTPDEKKTLAKKTGLTLTQVSNWFKNRRQRDRTPQQRPDIMSVLPVGQLDGNGFPRMFNAPSYYPETIFNGQ

Target ORF information:

RefSeq Version	NM_140999.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Six4, transcript variant A (Six4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140999.3

ORF Insert Sequence:

ATGTTTGACA AGAATTTGGA CGGCAACAAT CTGTCGGTCT CCATCGGTGG GGATTTGGAT 
TCGACGAGTT CCGGCGGCAC ATCCTCGGAT CACTCGGCTG TCCATCAGGA CAATCTCAGC 
TCGCCGATGG CCTACGGATC CCTCTTCCTC CCAAATGCTG GTTATCGTGG CAACCTGTCA 
TGCAAAACGG TCCTGCAGCT GGACAAGTTC GCACCCTACG AGGGTGTGGA GAAGGATCAC 
CTGCTGGAGC GGCGGTTTCA GGACATCACC AATGACTACG ACAAGTCGCC GCCGCCCACC 
GCCTCGACCA CGCCCACGCA CTATCCCAGC CTGAACAGCA TAATATTTGA GAATGGCAGC 
AGTGGCAACC TGGGCGATCT CAACGGGAAC ACGAAGACGG ATCTATGCGC CGGCCTGCAG 
AGAAGTGGCG GCGGATTGGG GGGCAATGCC GGCAGTGGTG GCCACCTCAT ATCCAATCTC 
ACAGCCGCCC ACAACATGTC CGCCGTGAGC AGCTTTCCTA TCGATGCCAA GATGCTGCAG 
TTCTCCACGG ATCAGATCCA GTGCATGTGC GAGGCACTGC AGCAGAAGGG CGACATCGAG 
AAGCTGACCA CATTCCTCTG CAGCCTGCCG CCCAGCGAGT TCTTCAAGAC CAACGAGAGT 
GTCCTGCGGG CCCGCGCCAT GGTGGCCTAC AATCTGGGCC AGTTCCACGA GCTGTACAAC 
CTGCTGGAGA CGCACTGCTT TTCGATCAAG TACCACGTGG ACCTGCAGAA CCTCTGGTTC 
AAGGCCCACT ACAAGGAGGC CGAGAAGGTG CGTGGCCGAC CACTGGGCGC CGTGGACAAG 
TACCGTCTGC GCAAGAAGTA TCCCCTGCCC AAGACGATCT GGGATGGCGA GGAGACGGTC 
TACTGCTTCA AGGAGAAGTC GCGCAACGCT CTCAAGGACT GCTACCTGAC CAACCGATAT 
CCCACCCCCG ACGAGAAGAA GACGCTGGCC AAGAAGACCG GGCTCACGCT CACCCAGGTC 
TCCAACTGGT TCAAGAATCG TCGACAGCGG GATCGCACGC CCCAGCAAAG ACCGGACATC 
ATGTCGGTGC TGCCCGTGGG CCAACTGGAT GGCAATGGAT TCCCGCGCAT GTTCAATGCT 
CCAAGCTATT ATCCCGAAAC GATTTTCAAT GGGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Six4 ( NM_168872.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730566.1 Drosophila melanogaster Six4, transcript variant B (Six4), mRNA.

Price & Availability↑

CloneID	OFa21166
Clone ID Related Accession (Same CDS sequence)	NM_140999.3 , NM_168872.1
Accession Version	NM_168872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Six4, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGACA AGAATTTGGA CGGCAACAAT CTGTCGGTCT CCATCGGTGG GGATTTGGAT 
TCGACGAGTT CCGGCGGCAC ATCCTCGGAT CACTCGGCTG TCCATCAGGA CAATCTCAGC 
TCGCCGATGG CCTACGGATC CCTCTTCCTC CCAAATGCTG GTTATCGTGG CAACCTGTCA 
TGCAAAACGG TCCTGCAGCT GGACAAGTTC GCACCCTACG AGGGTGTGGA GAAGGATCAC 
CTGCTGGAGC GGCGGTTTCA GGACATCACC AATGACTACG ACAAGTCGCC GCCGCCCACC 
GCCTCGACCA CGCCCACGCA CTATCCCAGC CTGAACAGCA TAATATTTGA GAATGGCAGC 
AGTGGCAACC TGGGCGATCT CAACGGGAAC ACGAAGACGG ATCTATGCGC CGGCCTGCAG 
AGAAGTGGCG GCGGATTGGG GGGCAATGCC GGCAGTGGTG GCCACCTCAT ATCCAATCTC 
ACAGCCGCCC ACAACATGTC CGCCGTGAGC AGCTTTCCTA TCGATGCCAA GATGCTGCAG 
TTCTCCACGG ATCAGATCCA GTGCATGTGC GAGGCACTGC AGCAGAAGGG CGACATCGAG 
AAGCTGACCA CATTCCTCTG CAGCCTGCCG CCCAGCGAGT TCTTCAAGAC CAACGAGAGT 
GTCCTGCGGG CCCGCGCCAT GGTGGCCTAC AATCTGGGCC AGTTCCACGA GCTGTACAAC 
CTGCTGGAGA CGCACTGCTT TTCGATCAAG TACCACGTGG ACCTGCAGAA CCTCTGGTTC 
AAGGCCCACT ACAAGGAGGC CGAGAAGGTG CGTGGCCGAC CACTGGGCGC CGTGGACAAG 
TACCGTCTGC GCAAGAAGTA TCCCCTGCCC AAGACGATCT GGGATGGCGA GGAGACGGTC 
TACTGCTTCA AGGAGAAGTC GCGCAACGCT CTCAAGGACT GCTACCTGAC CAACCGATAT 
CCCACCCCCG ACGAGAAGAA GACGCTGGCC AAGAAGACCG GGCTCACGCT CACCCAGGTC 
TCCAACTGGT TCAAGAATCG TCGACAGCGG GATCGCACGC CCCAGCAAAG ACCGGACATC 
ATGTCGGTGC TGCCCGTGGG CCAACTGGAT GGCAATGGAT TCCCGCGCAT GTTCAATGCT 
CCAAGCTATT ATCCCGAAAC GATTTTCAAT GGGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730566.1
CDS	68..1246
Translation	MFDKNLDGNNLSVSIGGDLDSTSSGGTSSDHSAVHQDNLSSPMAYGSLFLPNAGYRGNLSCKTVLQLDKFAPYEGVEKDHLLERRFQDITNDYDKSPPPTASTTPTHYPSLNSIIFENGSSGNLGDLNGNTKTDLCAGLQRSGGGLGGNAGSGGHLISNLTAAHNMSAVSSFPIDAKMLQFSTDQIQCMCEALQQKGDIEKLTTFLCSLPPSEFFKTNESVLRARAMVAYNLGQFHELYNLLETHCFSIKYHVDLQNLWFKAHYKEAEKVRGRPLGAVDKYRLRKKYPLPKTIWDGEETVYCFKEKSRNALKDCYLTNRYPTPDEKKTLAKKTGLTLTQVSNWFKNRRQRDRTPQQRPDIMSVLPVGQLDGNGFPRMFNAPSYYPETIFNGQ

Target ORF information:

RefSeq Version	NM_168872.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Six4, transcript variant B (Six4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168872.1

ORF Insert Sequence:

ATGTTTGACA AGAATTTGGA CGGCAACAAT CTGTCGGTCT CCATCGGTGG GGATTTGGAT 
TCGACGAGTT CCGGCGGCAC ATCCTCGGAT CACTCGGCTG TCCATCAGGA CAATCTCAGC 
TCGCCGATGG CCTACGGATC CCTCTTCCTC CCAAATGCTG GTTATCGTGG CAACCTGTCA 
TGCAAAACGG TCCTGCAGCT GGACAAGTTC GCACCCTACG AGGGTGTGGA GAAGGATCAC 
CTGCTGGAGC GGCGGTTTCA GGACATCACC AATGACTACG ACAAGTCGCC GCCGCCCACC 
GCCTCGACCA CGCCCACGCA CTATCCCAGC CTGAACAGCA TAATATTTGA GAATGGCAGC 
AGTGGCAACC TGGGCGATCT CAACGGGAAC ACGAAGACGG ATCTATGCGC CGGCCTGCAG 
AGAAGTGGCG GCGGATTGGG GGGCAATGCC GGCAGTGGTG GCCACCTCAT ATCCAATCTC 
ACAGCCGCCC ACAACATGTC CGCCGTGAGC AGCTTTCCTA TCGATGCCAA GATGCTGCAG 
TTCTCCACGG ATCAGATCCA GTGCATGTGC GAGGCACTGC AGCAGAAGGG CGACATCGAG 
AAGCTGACCA CATTCCTCTG CAGCCTGCCG CCCAGCGAGT TCTTCAAGAC CAACGAGAGT 
GTCCTGCGGG CCCGCGCCAT GGTGGCCTAC AATCTGGGCC AGTTCCACGA GCTGTACAAC 
CTGCTGGAGA CGCACTGCTT TTCGATCAAG TACCACGTGG ACCTGCAGAA CCTCTGGTTC 
AAGGCCCACT ACAAGGAGGC CGAGAAGGTG CGTGGCCGAC CACTGGGCGC CGTGGACAAG 
TACCGTCTGC GCAAGAAGTA TCCCCTGCCC AAGACGATCT GGGATGGCGA GGAGACGGTC 
TACTGCTTCA AGGAGAAGTC GCGCAACGCT CTCAAGGACT GCTACCTGAC CAACCGATAT 
CCCACCCCCG ACGAGAAGAA GACGCTGGCC AAGAAGACCG GGCTCACGCT CACCCAGGTC 
TCCAACTGGT TCAAGAATCG TCGACAGCGG GATCGCACGC CCCAGCAAAG ACCGGACATC 
ATGTCGGTGC TGCCCGTGGG CCAACTGGAT GGCAATGGAT TCCCGCGCAT GTTCAATGCT 
CCAAGCTATT ATCCCGAAAC GATTTTCAAT GGGCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Six4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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