Pitslre cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pitslre
Entrez Gene ID	40292
Full Name	CG4268 gene product from transcript CG4268-RA
Synonyms	CG4268,Dmel\CG4268,Gat,Gta,PITSLRE,PitsIre,cdk11,group 3,pitslre
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 77E6-77E6

mRNA and Protein(s)

mRNA	Protein	Name
NM_168869.3	NP_730563.1	pitslre, isoform C
NM_001259922.2	NP_001246851.1	pitslre, isoform E
NM_001259923.2	NP_001246852.1	pitslre, isoform F
NM_001275193.1	NP_001262122.1	pitslre, isoform G
NM_140994.4	NP_649251.2	pitslre, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Since PITSLRE/CDK11 regulates autophagy in both Drosophila and human cells, this kinase represents a novel phylogenetically conserved component of the autophagy machinery.
Title: The cyclin-dependent kinase PITSLRE/CDK11 is required for successful autophagy.

The following Pitslre gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pitslre cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19155	NM_168869.3 Latest version!	Drosophila melanogaster pitslre, transcript variant C (Pitslre), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa19155	NM_140994.4 Latest version!	Drosophila melanogaster pitslre, transcript variant A (Pitslre), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa19156	NM_001275193.1 Latest version!	Drosophila melanogaster pitslre, transcript variant G (Pitslre), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa19156	NM_001259923.2 Latest version!	Drosophila melanogaster pitslre, transcript variant F (Pitslre), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa19156	NM_001259922.2 Latest version!	Drosophila melanogaster pitslre, transcript variant E (Pitslre), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pitslre ( NM_168869.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730563.1 Drosophila melanogaster pitslre, transcript variant C (Pitslre), mRNA.

Price & Availability↑

CloneID	OFa19155
Clone ID Related Accession (Same CDS sequence)	NM_168869.3 , NM_140994.4
Accession Version	NM_168869.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2859bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pitslre, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168869.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCAATT CGTCTGGCAG CGAGGATGGC CAGTTGCGGA GCCCCAACGA TGTGCACTAT 
CACAGCCGAG GCGAGGAGGA TGAGCACGAG GGCGACGCTG ATGCCTTGTA CATTCAACCA 
CCGCAGGCCA GCCGAGAATC AGGATCCGGT CCCAGGCGCG AGAAGAAGAA ACATTCCCGC 
GAGCGTCGAC GCCACAAGGA GCGTGATGAC GTAGGCGGTG CTGCACTAGC ACTAGAACGG 
GATCATCGAT ATGACTATCG GAGCCGGGAG GAGCACTACC ACCACCACCA ACGCGAACGG 
AGCAGCAATG CGGCTGCAGC CTATGCGAAA CACCACCTGG GCCATGCCTA CCATTACCCT 
CAGCCGCCTC AACAGCAGCA ACAGCCTCTA CCGCCGGCGC CCAGCTATGC TGCCCACCAT 
TATCATCATC ACCAGCATTT AAGTGGAGCT CGAGCTGCTC CCCGCGAATA CCATTCCTAT 
CCATCGGGCT ACCATTCGGG CAGTCGACAC GGCGACTACC CAATGGAGGA GCCAACGCGA 
CGCAGCAGTA AGTACGCGGA GAGCAAGGAT GCTGAGAGCC TGGAACAGGA TCTGCGATCG 
AGGTTGCTGA AAAAGCGCCA TAACTACGTT AAGGATTATG AAACGGAGGA GAACTATGAG 
CATCGGGTCG AGAGAAGTGA TCGCCGGGAG GGAGGACGCA AAGAGCGGGA GAGAACTGTG 
CGAAGTACCC ACAAGCAAAA CCGTCACGAC CGAGTCATTG AGCTGCTGGA CAGTCCTGAG 
CAGGAGCACC ACCATCAGCA CCAGCACAAA TCGCATCGCA GCAAGTGGCG CGAGGAGGTT 
GAGGTTATCA GACGAAAGGT GCCCGAGGAC TTGGAGCTGT TGGCTCGCAG GGAAAAACTT 
CTTGCCGCTG AACGGGAAAG CCGCCAGCGA AAACAGACGG CTCGTGAGGA ACTGGAAGCT 
CGGCGGGAGT TGCTCCGCGA GCGAAACGAG CACAGTGATG CACTTAGTCC AACAACAGTG 
GCTGCAAGTG TAACCGCAGG TCTGAACATT CATGTGAAGA GGAAGTCCAA GCCGGATAAC 
TATGAGAAGG AAATCAAACT CAAGAAGCGG CGGGAAGATG ATATCGAGGT TATCCGAGAT 
GATGACGATG AGGAAAGCGA GGAGAGCGAC TCAAACGAGG AGGTTCCTGA GCAAGACAGC 
GAAGGAAGCG CAACAGAGTC TGGAAGCGAA GACAGCTATG CTAGCAAAAA GAAGAGCAAG 
ATTAAATCCA AGTCTCAATT GGAAGACGAT GACGAGGATT TACCCTTACC CGACAGTCCA 
CTTAGCGTGG GTGAGCTATA CAAGTCGCCC AAGCAACGTC AGCGATCTCG CTCTGTAAGC 
TCCAAGAGCA GCTCCCAGTC CAGTCGAAGC AGTCGGTCCA GATCCCGTTC TAGAAGTCAA 
AGCAGCCTAG AGGATGAGGT AGATCGTCAA GACGTGGGGG CAGATGCCTC ACCCAGCAGC 
AGCACACGCA GCGAGGAACG TGGAATGACC CAAGAGCAGC CGGAAGAGAA GCCCGAGGAG 
AAACTCAAAG AGAAACAGAA GTCTCTGGAG GAGCAAATTC CGTGCGATGA CAAGGGCATT 
CCCCTGCCCA ACTATTATCC CGGTGTGCAG GGCTGCCGGT CTGTGGAGGA ATTCCAGTGT 
CTAAACCGCA TCGAAGAGGG AACCTATGGA GTGGTATATC GAGCCAAGGA CAAAAGAACC 
AATGAGATTG TGGCTCTTAA GCGTCTGAAA ATGGAGAAGG AAAAGGAGGG ATTTCCGATA 
ACATCACTCA GGGAGATCAA CACTCTTCTC AAGGGTCAGC ACCCGAATAT TGTGACCGTT 
CGCGAGATCG TGGTCGGATC CAACATGGAT AAGATATTTA TTGTGATGGA CTATGTAGAA 
CACGACCTGA AATCCCTGAT GGAAACGATG AAAAATCGTA AGCAGAGCTT TTTCCCCGGA 
GAAGTCAAAT GTCTGACGCA GCAGCTTCTG CGGGCGGTGG CCCACCTCCA TGACAATTGG 
ATATTGCATC GTGACCTGAA GACCTCCAAT CTCCTCTTGT CCCACAAGGG CATTCTCAAG 
GTGGGTGATT TCGGATTGGC AAGGGAGTAT GGATCGCCGA TCAAGAAGTA TACCTCACTT 
GTTGTCACTC TCTGGTATCG TGCACCCGAG CTCCTTCTCT GCTCGCCGGT TTACTCCACG 
CCCATAGACG TCTGGTCGGT AGGCTGCATT TTCGCCGAAT TCTTGCAGAT GCTGCCGCTC 
TTTCCGGGAA AATCTGAAAT CGATGAGCTT AACAGGATCT TTAAGGAATT GGGAACTCCG 
AACGAGAAGA TCTGGCCAGG ATACACCGAG CTCCCAGCCG TGAAGAACAT GTTGTCGCAG 
AACTCTCAGT TTACCGAGTA TCCCGTCTCG CAACTGCGCA AGCACTTCCA GGAGAAGACC 
TCTGAAATGG GCTTGTCGCT GCTGCAGGGT CTGCTGACGT ACGATCCGAA ACAAAGACTG 
AGCGCCGACG CGGCACTAAA GCACGGCTTC TTCAAGGAGC TGCCATTGCC CATCGATCCA 
TCCATGTTTC CCACCTGGCC GGCCAAGAGC GAGTTGGGAG CCCGCAAGGC GCAAGCCTCT 
TCCCCCAAAC CGCCATCCGG TGGCTCGCAG TTCAAGCAAC TAGGCCGCGA CGAGCCCATC 
ATCGTGGGAC CGGGAAACAA GCTCTCATCT GGGATTATCA CTGGCAACAA GAAGAGCCAC 
GGAGCTGGTG GATCGTCGGC TAGCACGGGA TTTGTCCTGA ACGCTGGAAT AACGCAGCGC 
CAGTTGGCCA TGGGACCGGG ATTCAGTCTG AAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730563.1
CDS	214..3072
Translation	MVNSSGSEDGQLRSPNDVHYHSRGEEDEHEGDADALYIQPPQASRESGSGPRREKKKHSRERRRHKERDDVGGAALALERDHRYDYRSREEHYHHHQRERSSNAAAAYAKHHLGHAYHYPQPPQQQQQPLPPAPSYAAHHYHHHQHLSGARAAPREYHSYPSGYHSGSRHGDYPMEEPTRRSSKYAESKDAESLEQDLRSRLLKKRHNYVKDYETEENYEHRVERSDRREGGRKERERTVRSTHKQNRHDRVIELLDSPEQEHHHQHQHKSHRSKWREEVEVIRRKVPEDLELLARREKLLAAERESRQRKQTAREELEARRELLRERNEHSDALSPTTVAASVTAGLNIHVKRKSKPDNYEKEIKLKKRREDDIEVIRDDDDEESEESDSNEEVPEQDSEGSATESGSEDSYASKKKSKIKSKSQLEDDDEDLPLPDSPLSVGELYKSPKQRQRSRSVSSKSSSQSSRSSRSRSRSRSQSSLEDEVDRQDVGADASPSSSTRSEERGMTQEQPEEKPEEKLKEKQKSLEEQIPCDDKGIPLPNYYPGVQGCRSVEEFQCLNRIEEGTYGVVYRAKDKRTNEIVALKRLKMEKEKEGFPITSLREINTLLKGQHPNIVTVREIVVGSNMDKIFIVMDYVEHDLKSLMETMKNRKQSFFPGEVKCLTQQLLRAVAHLHDNWILHRDLKTSNLLLSHKGILKVGDFGLAREYGSPIKKYTSLVVTLWYRAPELLLCSPVYSTPIDVWSVGCIFAEFLQMLPLFPGKSEIDELNRIFKELGTPNEKIWPGYTELPAVKNMLSQNSQFTEYPVSQLRKHFQEKTSEMGLSLLQGLLTYDPKQRLSADAALKHGFFKELPLPIDPSMFPTWPAKSELGARKAQASSPKPPSGGSQFKQLGRDEPIIVGPGNKLSSGIITGNKKSHGAGGSSASTGFVLNAGITQRQLAMGPGFSLKF

Target ORF information:

RefSeq Version	NM_168869.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pitslre, transcript variant C (Pitslre), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168869.3

ORF Insert Sequence:

ATGGTCAATT CGTCTGGCAG CGAGGATGGC CAGTTGCGGA GCCCCAACGA TGTGCACTAT 
CACAGCCGAG GCGAGGAGGA TGAGCACGAG GGCGACGCTG ATGCCTTGTA CATTCAACCA 
CCGCAGGCCA GCCGAGAATC AGGATCCGGT CCCAGGCGCG AGAAGAAGAA ACATTCCCGC 
GAGCGTCGAC GCCACAAGGA GCGTGATGAC GTAGGCGGTG CTGCACTAGC ACTAGAACGG 
GATCATCGAT ATGACTATCG GAGCCGGGAG GAGCACTACC ACCACCACCA ACGCGAACGG 
AGCAGCAATG CGGCTGCAGC CTATGCGAAA CACCACCTGG GCCATGCCTA CCATTACCCT 
CAGCCGCCTC AACAGCAGCA ACAGCCTCTA CCGCCGGCGC CCAGCTATGC TGCCCACCAT 
TATCATCATC ACCAGCATTT AAGTGGAGCT CGAGCTGCTC CCCGCGAATA CCATTCCTAT 
CCATCGGGCT ACCATTCGGG CAGTCGACAC GGCGACTACC CAATGGAGGA GCCAACGCGA 
CGCAGCAGTA AGTACGCGGA GAGCAAGGAT GCTGAGAGCC TGGAACAGGA TCTGCGATCG 
AGGTTGCTGA AAAAGCGCCA TAACTACGTT AAGGATTATG AAACGGAGGA GAACTATGAG 
CATCGGGTCG AGAGAAGTGA TCGCCGGGAG GGAGGACGCA AAGAGCGGGA GAGAACTGTG 
CGAAGTACCC ACAAGCAAAA CCGTCACGAC CGAGTCATTG AGCTGCTGGA CAGTCCTGAG 
CAGGAGCACC ACCATCAGCA CCAGCACAAA TCGCATCGCA GCAAGTGGCG CGAGGAGGTT 
GAGGTTATCA GACGAAAGGT GCCCGAGGAC TTGGAGCTGT TGGCTCGCAG GGAAAAACTT 
CTTGCCGCTG AACGGGAAAG CCGCCAGCGA AAACAGACGG CTCGTGAGGA ACTGGAAGCT 
CGGCGGGAGT TGCTCCGCGA GCGAAACGAG CACAGTGATG CACTTAGTCC AACAACAGTG 
GCTGCAAGTG TAACCGCAGG TCTGAACATT CATGTGAAGA GGAAGTCCAA GCCGGATAAC 
TATGAGAAGG AAATCAAACT CAAGAAGCGG CGGGAAGATG ATATCGAGGT TATCCGAGAT 
GATGACGATG AGGAAAGCGA GGAGAGCGAC TCAAACGAGG AGGTTCCTGA GCAAGACAGC 
GAAGGAAGCG CAACAGAGTC TGGAAGCGAA GACAGCTATG CTAGCAAAAA GAAGAGCAAG 
ATTAAATCCA AGTCTCAATT GGAAGACGAT GACGAGGATT TACCCTTACC CGACAGTCCA 
CTTAGCGTGG GTGAGCTATA CAAGTCGCCC AAGCAACGTC AGCGATCTCG CTCTGTAAGC 
TCCAAGAGCA GCTCCCAGTC CAGTCGAAGC AGTCGGTCCA GATCCCGTTC TAGAAGTCAA 
AGCAGCCTAG AGGATGAGGT AGATCGTCAA GACGTGGGGG CAGATGCCTC ACCCAGCAGC 
AGCACACGCA GCGAGGAACG TGGAATGACC CAAGAGCAGC CGGAAGAGAA GCCCGAGGAG 
AAACTCAAAG AGAAACAGAA GTCTCTGGAG GAGCAAATTC CGTGCGATGA CAAGGGCATT 
CCCCTGCCCA ACTATTATCC CGGTGTGCAG GGCTGCCGGT CTGTGGAGGA ATTCCAGTGT 
CTAAACCGCA TCGAAGAGGG AACCTATGGA GTGGTATATC GAGCCAAGGA CAAAAGAACC 
AATGAGATTG TGGCTCTTAA GCGTCTGAAA ATGGAGAAGG AAAAGGAGGG ATTTCCGATA 
ACATCACTCA GGGAGATCAA CACTCTTCTC AAGGGTCAGC ACCCGAATAT TGTGACCGTT 
CGCGAGATCG TGGTCGGATC CAACATGGAT AAGATATTTA TTGTGATGGA CTATGTAGAA 
CACGACCTGA AATCCCTGAT GGAAACGATG AAAAATCGTA AGCAGAGCTT TTTCCCCGGA 
GAAGTCAAAT GTCTGACGCA GCAGCTTCTG CGGGCGGTGG CCCACCTCCA TGACAATTGG 
ATATTGCATC GTGACCTGAA GACCTCCAAT CTCCTCTTGT CCCACAAGGG CATTCTCAAG 
GTGGGTGATT TCGGATTGGC AAGGGAGTAT GGATCGCCGA TCAAGAAGTA TACCTCACTT 
GTTGTCACTC TCTGGTATCG TGCACCCGAG CTCCTTCTCT GCTCGCCGGT TTACTCCACG 
CCCATAGACG TCTGGTCGGT AGGCTGCATT TTCGCCGAAT TCTTGCAGAT GCTGCCGCTC 
TTTCCGGGAA AATCTGAAAT CGATGAGCTT AACAGGATCT TTAAGGAATT GGGAACTCCG 
AACGAGAAGA TCTGGCCAGG ATACACCGAG CTCCCAGCCG TGAAGAACAT GTTGTCGCAG 
AACTCTCAGT TTACCGAGTA TCCCGTCTCG CAACTGCGCA AGCACTTCCA GGAGAAGACC 
TCTGAAATGG GCTTGTCGCT GCTGCAGGGT CTGCTGACGT ACGATCCGAA ACAAAGACTG 
AGCGCCGACG CGGCACTAAA GCACGGCTTC TTCAAGGAGC TGCCATTGCC CATCGATCCA 
TCCATGTTTC CCACCTGGCC GGCCAAGAGC GAGTTGGGAG CCCGCAAGGC GCAAGCCTCT 
TCCCCCAAAC CGCCATCCGG TGGCTCGCAG TTCAAGCAAC TAGGCCGCGA CGAGCCCATC 
ATCGTGGGAC CGGGAAACAA GCTCTCATCT GGGATTATCA CTGGCAACAA GAAGAGCCAC 
GGAGCTGGTG GATCGTCGGC TAGCACGGGA TTTGTCCTGA ACGCTGGAAT AACGCAGCGC 
CAGTTGGCCA TGGGACCGGG ATTCAGTCTG AAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pitslre ( NM_140994.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649251.2 Drosophila melanogaster pitslre, transcript variant A (Pitslre), mRNA.

Price & Availability↑

CloneID	OFa19155
Clone ID Related Accession (Same CDS sequence)	NM_168869.3 , NM_140994.4
Accession Version	NM_140994.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2859bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pitslre, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140994.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCAATT CGTCTGGCAG CGAGGATGGC CAGTTGCGGA GCCCCAACGA TGTGCACTAT 
CACAGCCGAG GCGAGGAGGA TGAGCACGAG GGCGACGCTG ATGCCTTGTA CATTCAACCA 
CCGCAGGCCA GCCGAGAATC AGGATCCGGT CCCAGGCGCG AGAAGAAGAA ACATTCCCGC 
GAGCGTCGAC GCCACAAGGA GCGTGATGAC GTAGGCGGTG CTGCACTAGC ACTAGAACGG 
GATCATCGAT ATGACTATCG GAGCCGGGAG GAGCACTACC ACCACCACCA ACGCGAACGG 
AGCAGCAATG CGGCTGCAGC CTATGCGAAA CACCACCTGG GCCATGCCTA CCATTACCCT 
CAGCCGCCTC AACAGCAGCA ACAGCCTCTA CCGCCGGCGC CCAGCTATGC TGCCCACCAT 
TATCATCATC ACCAGCATTT AAGTGGAGCT CGAGCTGCTC CCCGCGAATA CCATTCCTAT 
CCATCGGGCT ACCATTCGGG CAGTCGACAC GGCGACTACC CAATGGAGGA GCCAACGCGA 
CGCAGCAGTA AGTACGCGGA GAGCAAGGAT GCTGAGAGCC TGGAACAGGA TCTGCGATCG 
AGGTTGCTGA AAAAGCGCCA TAACTACGTT AAGGATTATG AAACGGAGGA GAACTATGAG 
CATCGGGTCG AGAGAAGTGA TCGCCGGGAG GGAGGACGCA AAGAGCGGGA GAGAACTGTG 
CGAAGTACCC ACAAGCAAAA CCGTCACGAC CGAGTCATTG AGCTGCTGGA CAGTCCTGAG 
CAGGAGCACC ACCATCAGCA CCAGCACAAA TCGCATCGCA GCAAGTGGCG CGAGGAGGTT 
GAGGTTATCA GACGAAAGGT GCCCGAGGAC TTGGAGCTGT TGGCTCGCAG GGAAAAACTT 
CTTGCCGCTG AACGGGAAAG CCGCCAGCGA AAACAGACGG CTCGTGAGGA ACTGGAAGCT 
CGGCGGGAGT TGCTCCGCGA GCGAAACGAG CACAGTGATG CACTTAGTCC AACAACAGTG 
GCTGCAAGTG TAACCGCAGG TCTGAACATT CATGTGAAGA GGAAGTCCAA GCCGGATAAC 
TATGAGAAGG AAATCAAACT CAAGAAGCGG CGGGAAGATG ATATCGAGGT TATCCGAGAT 
GATGACGATG AGGAAAGCGA GGAGAGCGAC TCAAACGAGG AGGTTCCTGA GCAAGACAGC 
GAAGGAAGCG CAACAGAGTC TGGAAGCGAA GACAGCTATG CTAGCAAAAA GAAGAGCAAG 
ATTAAATCCA AGTCTCAATT GGAAGACGAT GACGAGGATT TACCCTTACC CGACAGTCCA 
CTTAGCGTGG GTGAGCTATA CAAGTCGCCC AAGCAACGTC AGCGATCTCG CTCTGTAAGC 
TCCAAGAGCA GCTCCCAGTC CAGTCGAAGC AGTCGGTCCA GATCCCGTTC TAGAAGTCAA 
AGCAGCCTAG AGGATGAGGT AGATCGTCAA GACGTGGGGG CAGATGCCTC ACCCAGCAGC 
AGCACACGCA GCGAGGAACG TGGAATGACC CAAGAGCAGC CGGAAGAGAA GCCCGAGGAG 
AAACTCAAAG AGAAACAGAA GTCTCTGGAG GAGCAAATTC CGTGCGATGA CAAGGGCATT 
CCCCTGCCCA ACTATTATCC CGGTGTGCAG GGCTGCCGGT CTGTGGAGGA ATTCCAGTGT 
CTAAACCGCA TCGAAGAGGG AACCTATGGA GTGGTATATC GAGCCAAGGA CAAAAGAACC 
AATGAGATTG TGGCTCTTAA GCGTCTGAAA ATGGAGAAGG AAAAGGAGGG ATTTCCGATA 
ACATCACTCA GGGAGATCAA CACTCTTCTC AAGGGTCAGC ACCCGAATAT TGTGACCGTT 
CGCGAGATCG TGGTCGGATC CAACATGGAT AAGATATTTA TTGTGATGGA CTATGTAGAA 
CACGACCTGA AATCCCTGAT GGAAACGATG AAAAATCGTA AGCAGAGCTT TTTCCCCGGA 
GAAGTCAAAT GTCTGACGCA GCAGCTTCTG CGGGCGGTGG CCCACCTCCA TGACAATTGG 
ATATTGCATC GTGACCTGAA GACCTCCAAT CTCCTCTTGT CCCACAAGGG CATTCTCAAG 
GTGGGTGATT TCGGATTGGC AAGGGAGTAT GGATCGCCGA TCAAGAAGTA TACCTCACTT 
GTTGTCACTC TCTGGTATCG TGCACCCGAG CTCCTTCTCT GCTCGCCGGT TTACTCCACG 
CCCATAGACG TCTGGTCGGT AGGCTGCATT TTCGCCGAAT TCTTGCAGAT GCTGCCGCTC 
TTTCCGGGAA AATCTGAAAT CGATGAGCTT AACAGGATCT TTAAGGAATT GGGAACTCCG 
AACGAGAAGA TCTGGCCAGG ATACACCGAG CTCCCAGCCG TGAAGAACAT GTTGTCGCAG 
AACTCTCAGT TTACCGAGTA TCCCGTCTCG CAACTGCGCA AGCACTTCCA GGAGAAGACC 
TCTGAAATGG GCTTGTCGCT GCTGCAGGGT CTGCTGACGT ACGATCCGAA ACAAAGACTG 
AGCGCCGACG CGGCACTAAA GCACGGCTTC TTCAAGGAGC TGCCATTGCC CATCGATCCA 
TCCATGTTTC CCACCTGGCC GGCCAAGAGC GAGTTGGGAG CCCGCAAGGC GCAAGCCTCT 
TCCCCCAAAC CGCCATCCGG TGGCTCGCAG TTCAAGCAAC TAGGCCGCGA CGAGCCCATC 
ATCGTGGGAC CGGGAAACAA GCTCTCATCT GGGATTATCA CTGGCAACAA GAAGAGCCAC 
GGAGCTGGTG GATCGTCGGC TAGCACGGGA TTTGTCCTGA ACGCTGGAAT AACGCAGCGC 
CAGTTGGCCA TGGGACCGGG ATTCAGTCTG AAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649251.2
CDS	273..3131
Translation	MVNSSGSEDGQLRSPNDVHYHSRGEEDEHEGDADALYIQPPQASRESGSGPRREKKKHSRERRRHKERDDVGGAALALERDHRYDYRSREEHYHHHQRERSSNAAAAYAKHHLGHAYHYPQPPQQQQQPLPPAPSYAAHHYHHHQHLSGARAAPREYHSYPSGYHSGSRHGDYPMEEPTRRSSKYAESKDAESLEQDLRSRLLKKRHNYVKDYETEENYEHRVERSDRREGGRKERERTVRSTHKQNRHDRVIELLDSPEQEHHHQHQHKSHRSKWREEVEVIRRKVPEDLELLARREKLLAAERESRQRKQTAREELEARRELLRERNEHSDALSPTTVAASVTAGLNIHVKRKSKPDNYEKEIKLKKRREDDIEVIRDDDDEESEESDSNEEVPEQDSEGSATESGSEDSYASKKKSKIKSKSQLEDDDEDLPLPDSPLSVGELYKSPKQRQRSRSVSSKSSSQSSRSSRSRSRSRSQSSLEDEVDRQDVGADASPSSSTRSEERGMTQEQPEEKPEEKLKEKQKSLEEQIPCDDKGIPLPNYYPGVQGCRSVEEFQCLNRIEEGTYGVVYRAKDKRTNEIVALKRLKMEKEKEGFPITSLREINTLLKGQHPNIVTVREIVVGSNMDKIFIVMDYVEHDLKSLMETMKNRKQSFFPGEVKCLTQQLLRAVAHLHDNWILHRDLKTSNLLLSHKGILKVGDFGLAREYGSPIKKYTSLVVTLWYRAPELLLCSPVYSTPIDVWSVGCIFAEFLQMLPLFPGKSEIDELNRIFKELGTPNEKIWPGYTELPAVKNMLSQNSQFTEYPVSQLRKHFQEKTSEMGLSLLQGLLTYDPKQRLSADAALKHGFFKELPLPIDPSMFPTWPAKSELGARKAQASSPKPPSGGSQFKQLGRDEPIIVGPGNKLSSGIITGNKKSHGAGGSSASTGFVLNAGITQRQLAMGPGFSLKF

Target ORF information:

RefSeq Version	NM_140994.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pitslre, transcript variant A (Pitslre), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140994.4

ORF Insert Sequence:

ATGGTCAATT CGTCTGGCAG CGAGGATGGC CAGTTGCGGA GCCCCAACGA TGTGCACTAT 
CACAGCCGAG GCGAGGAGGA TGAGCACGAG GGCGACGCTG ATGCCTTGTA CATTCAACCA 
CCGCAGGCCA GCCGAGAATC AGGATCCGGT CCCAGGCGCG AGAAGAAGAA ACATTCCCGC 
GAGCGTCGAC GCCACAAGGA GCGTGATGAC GTAGGCGGTG CTGCACTAGC ACTAGAACGG 
GATCATCGAT ATGACTATCG GAGCCGGGAG GAGCACTACC ACCACCACCA ACGCGAACGG 
AGCAGCAATG CGGCTGCAGC CTATGCGAAA CACCACCTGG GCCATGCCTA CCATTACCCT 
CAGCCGCCTC AACAGCAGCA ACAGCCTCTA CCGCCGGCGC CCAGCTATGC TGCCCACCAT 
TATCATCATC ACCAGCATTT AAGTGGAGCT CGAGCTGCTC CCCGCGAATA CCATTCCTAT 
CCATCGGGCT ACCATTCGGG CAGTCGACAC GGCGACTACC CAATGGAGGA GCCAACGCGA 
CGCAGCAGTA AGTACGCGGA GAGCAAGGAT GCTGAGAGCC TGGAACAGGA TCTGCGATCG 
AGGTTGCTGA AAAAGCGCCA TAACTACGTT AAGGATTATG AAACGGAGGA GAACTATGAG 
CATCGGGTCG AGAGAAGTGA TCGCCGGGAG GGAGGACGCA AAGAGCGGGA GAGAACTGTG 
CGAAGTACCC ACAAGCAAAA CCGTCACGAC CGAGTCATTG AGCTGCTGGA CAGTCCTGAG 
CAGGAGCACC ACCATCAGCA CCAGCACAAA TCGCATCGCA GCAAGTGGCG CGAGGAGGTT 
GAGGTTATCA GACGAAAGGT GCCCGAGGAC TTGGAGCTGT TGGCTCGCAG GGAAAAACTT 
CTTGCCGCTG AACGGGAAAG CCGCCAGCGA AAACAGACGG CTCGTGAGGA ACTGGAAGCT 
CGGCGGGAGT TGCTCCGCGA GCGAAACGAG CACAGTGATG CACTTAGTCC AACAACAGTG 
GCTGCAAGTG TAACCGCAGG TCTGAACATT CATGTGAAGA GGAAGTCCAA GCCGGATAAC 
TATGAGAAGG AAATCAAACT CAAGAAGCGG CGGGAAGATG ATATCGAGGT TATCCGAGAT 
GATGACGATG AGGAAAGCGA GGAGAGCGAC TCAAACGAGG AGGTTCCTGA GCAAGACAGC 
GAAGGAAGCG CAACAGAGTC TGGAAGCGAA GACAGCTATG CTAGCAAAAA GAAGAGCAAG 
ATTAAATCCA AGTCTCAATT GGAAGACGAT GACGAGGATT TACCCTTACC CGACAGTCCA 
CTTAGCGTGG GTGAGCTATA CAAGTCGCCC AAGCAACGTC AGCGATCTCG CTCTGTAAGC 
TCCAAGAGCA GCTCCCAGTC CAGTCGAAGC AGTCGGTCCA GATCCCGTTC TAGAAGTCAA 
AGCAGCCTAG AGGATGAGGT AGATCGTCAA GACGTGGGGG CAGATGCCTC ACCCAGCAGC 
AGCACACGCA GCGAGGAACG TGGAATGACC CAAGAGCAGC CGGAAGAGAA GCCCGAGGAG 
AAACTCAAAG AGAAACAGAA GTCTCTGGAG GAGCAAATTC CGTGCGATGA CAAGGGCATT 
CCCCTGCCCA ACTATTATCC CGGTGTGCAG GGCTGCCGGT CTGTGGAGGA ATTCCAGTGT 
CTAAACCGCA TCGAAGAGGG AACCTATGGA GTGGTATATC GAGCCAAGGA CAAAAGAACC 
AATGAGATTG TGGCTCTTAA GCGTCTGAAA ATGGAGAAGG AAAAGGAGGG ATTTCCGATA 
ACATCACTCA GGGAGATCAA CACTCTTCTC AAGGGTCAGC ACCCGAATAT TGTGACCGTT 
CGCGAGATCG TGGTCGGATC CAACATGGAT AAGATATTTA TTGTGATGGA CTATGTAGAA 
CACGACCTGA AATCCCTGAT GGAAACGATG AAAAATCGTA AGCAGAGCTT TTTCCCCGGA 
GAAGTCAAAT GTCTGACGCA GCAGCTTCTG CGGGCGGTGG CCCACCTCCA TGACAATTGG 
ATATTGCATC GTGACCTGAA GACCTCCAAT CTCCTCTTGT CCCACAAGGG CATTCTCAAG 
GTGGGTGATT TCGGATTGGC AAGGGAGTAT GGATCGCCGA TCAAGAAGTA TACCTCACTT 
GTTGTCACTC TCTGGTATCG TGCACCCGAG CTCCTTCTCT GCTCGCCGGT TTACTCCACG 
CCCATAGACG TCTGGTCGGT AGGCTGCATT TTCGCCGAAT TCTTGCAGAT GCTGCCGCTC 
TTTCCGGGAA AATCTGAAAT CGATGAGCTT AACAGGATCT TTAAGGAATT GGGAACTCCG 
AACGAGAAGA TCTGGCCAGG ATACACCGAG CTCCCAGCCG TGAAGAACAT GTTGTCGCAG 
AACTCTCAGT TTACCGAGTA TCCCGTCTCG CAACTGCGCA AGCACTTCCA GGAGAAGACC 
TCTGAAATGG GCTTGTCGCT GCTGCAGGGT CTGCTGACGT ACGATCCGAA ACAAAGACTG 
AGCGCCGACG CGGCACTAAA GCACGGCTTC TTCAAGGAGC TGCCATTGCC CATCGATCCA 
TCCATGTTTC CCACCTGGCC GGCCAAGAGC GAGTTGGGAG CCCGCAAGGC GCAAGCCTCT 
TCCCCCAAAC CGCCATCCGG TGGCTCGCAG TTCAAGCAAC TAGGCCGCGA CGAGCCCATC 
ATCGTGGGAC CGGGAAACAA GCTCTCATCT GGGATTATCA CTGGCAACAA GAAGAGCCAC 
GGAGCTGGTG GATCGTCGGC TAGCACGGGA TTTGTCCTGA ACGCTGGAAT AACGCAGCGC 
CAGTTGGCCA TGGGACCGGG ATTCAGTCTG AAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pitslre ( NM_001259922.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246851.1 Drosophila melanogaster pitslre, transcript variant E (Pitslre), mRNA.

Price & Availability↑

CloneID	OFa19156
Clone ID Related Accession (Same CDS sequence)	NM_001259922.2 , NM_001259923.2 , NM_001275193.1
Accession Version	NM_001259922.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pitslre, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001259922.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAGC CAACGCGACG CAGCAGTAAG TACGCGGAGA GCAAGGATGC TGAGAGCCTG 
GAACAGGATC TGCGATCGAG GTTGCTGAAA AAGCGCCATA ACTACGTTAA GGATTATGAA 
ACGGAGGAGA ACTATGAGCA TCGGGTCGAG AGAAGTGATC GCCGGGAGGG AGGACGCAAA 
GAGCGGGAGA GAACTGTGCG AAGTACCCAC AAGCAAAACC GTCACGACCG AGTCATTGAG 
CTGCTGGACA GTCCTGAGCA GGAGCACCAC CATCAGCACC AGCACAAATC GCATCGCAGC 
AAGTGGCGCG AGGAGGTTGA GGTTATCAGA CGAAAGGTGC CCGAGGACTT GGAGCTGTTG 
GCTCGCAGGG AAAAACTTCT TGCCGCTGAA CGGGAAAGCC GCCAGCGAAA ACAGACGGCT 
CGTGAGGAAC TGGAAGCTCG GCGGGAGTTG CTCCGCGAGC GAAACGAGCA CAGTGATGCA 
CTTAGTCCAA CAACAGTGGC TGCAAGTGTA ACCGCAGGTC TGAACATTCA TGTGAAGAGG 
AAGTCCAAGC CGGATAACTA TGAGAAGGAA ATCAAACTCA AGAAGCGGCG GGAAGATGAT 
ATCGAGGTTA TCCGAGATGA TGACGATGAG GAAAGCGAGG AGAGCGACTC AAACGAGGAG 
GTTCCTGAGC AAGACAGCGA AGGAAGCGCA ACAGAGTCTG GAAGCGAAGA CAGCTATGCT 
AGCAAAAAGA AGAGCAAGAT TAAATCCAAG TCTCAATTGG AAGACGATGA CGAGGATTTA 
CCCTTACCCG ACAGTCCACT TAGCGTGGGT GAGCTATACA AGTCGCCCAA GCAACGTCAG 
CGATCTCGCT CTGTAAGCTC CAAGAGCAGC TCCCAGTCCA GTCGAAGCAG TCGGTCCAGA 
TCCCGTTCTA GAAGTCAAAG CAGCCTAGAG GATGAGGTAG ATCGTCAAGA CGTGGGGGCA 
GATGCCTCAC CCAGCAGCAG CACACGCAGC GAGGAACGTG GAATGACCCA AGAGCAGCCG 
GAAGAGAAGC CCGAGGAGAA ACTCAAAGAG AAACAGAAGT CTCTGGAGGA GCAAATTCCG 
TGCGATGACA AGGGCATTCC CCTGCCCAAC TATTATCCCG GTGTGCAGGG CTGCCGGTCT 
GTGGAGGAAT TCCAGTGTCT AAACCGCATC GAAGAGGGAA CCTATGGAGT GGTATATCGA 
GCCAAGGACA AAAGAACCAA TGAGATTGTG GCTCTTAAGC GTCTGAAAAT GGAGAAGGAA 
AAGGAGGGAT TTCCGATAAC ATCACTCAGG GAGATCAACA CTCTTCTCAA GGGTCAGCAC 
CCGAATATTG TGACCGTTCG CGAGATCGTG GTCGGATCCA ACATGGATAA GATATTTATT 
GTGATGGACT ATGTAGAACA CGACCTGAAA TCCCTGATGG AAACGATGAA AAATCGTAAG 
CAGAGCTTTT TCCCCGGAGA AGTCAAATGT CTGACGCAGC AGCTTCTGCG GGCGGTGGCC 
CACCTCCATG ACAATTGGAT ATTGCATCGT GACCTGAAGA CCTCCAATCT CCTCTTGTCC 
CACAAGGGCA TTCTCAAGGT GGGTGATTTC GGATTGGCAA GGGAGTATGG ATCGCCGATC 
AAGAAGTATA CCTCACTTGT TGTCACTCTC TGGTATCGTG CACCCGAGCT CCTTCTCTGC 
TCGCCGGTTT ACTCCACGCC CATAGACGTC TGGTCGGTAG GCTGCATTTT CGCCGAATTC 
TTGCAGATGC TGCCGCTCTT TCCGGGAAAA TCTGAAATCG ATGAGCTTAA CAGGATCTTT 
AAGGAATTGG GAACTCCGAA CGAGAAGATC TGGCCAGGAT ACACCGAGCT CCCAGCCGTG 
AAGAACATGT TGTCGCAGAA CTCTCAGTTT ACCGAGTATC CCGTCTCGCA ACTGCGCAAG 
CACTTCCAGG AGAAGACCTC TGAAATGGGC TTGTCGCTGC TGCAGGGTCT GCTGACGTAC 
GATCCGAAAC AAAGACTGAG CGCCGACGCG GCACTAAAGC ACGGCTTCTT CAAGGAGCTG 
CCATTGCCCA TCGATCCATC CATGTTTCCC ACCTGGCCGG CCAAGAGCGA GTTGGGAGCC 
CGCAAGGCGC AAGCCTCTTC CCCCAAACCG CCATCCGGTG GCTCGCAGTT CAAGCAACTA 
GGCCGCGACG AGCCCATCAT CGTGGGACCG GGAAACAAGC TCTCATCTGG GATTATCACT 
GGCAACAAGA AGAGCCACGG AGCTGGTGGA TCGTCGGCTA GCACGGGATT TGTCCTGAAC 
GCTGGAATAA CGCAGCGCCA GTTGGCCATG GGACCGGGAT TCAGTCTGAA GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246851.1
CDS	1925..4261
Translation	MEEPTRRSSKYAESKDAESLEQDLRSRLLKKRHNYVKDYETEENYEHRVERSDRREGGRKERERTVRSTHKQNRHDRVIELLDSPEQEHHHQHQHKSHRSKWREEVEVIRRKVPEDLELLARREKLLAAERESRQRKQTAREELEARRELLRERNEHSDALSPTTVAASVTAGLNIHVKRKSKPDNYEKEIKLKKRREDDIEVIRDDDDEESEESDSNEEVPEQDSEGSATESGSEDSYASKKKSKIKSKSQLEDDDEDLPLPDSPLSVGELYKSPKQRQRSRSVSSKSSSQSSRSSRSRSRSRSQSSLEDEVDRQDVGADASPSSSTRSEERGMTQEQPEEKPEEKLKEKQKSLEEQIPCDDKGIPLPNYYPGVQGCRSVEEFQCLNRIEEGTYGVVYRAKDKRTNEIVALKRLKMEKEKEGFPITSLREINTLLKGQHPNIVTVREIVVGSNMDKIFIVMDYVEHDLKSLMETMKNRKQSFFPGEVKCLTQQLLRAVAHLHDNWILHRDLKTSNLLLSHKGILKVGDFGLAREYGSPIKKYTSLVVTLWYRAPELLLCSPVYSTPIDVWSVGCIFAEFLQMLPLFPGKSEIDELNRIFKELGTPNEKIWPGYTELPAVKNMLSQNSQFTEYPVSQLRKHFQEKTSEMGLSLLQGLLTYDPKQRLSADAALKHGFFKELPLPIDPSMFPTWPAKSELGARKAQASSPKPPSGGSQFKQLGRDEPIIVGPGNKLSSGIITGNKKSHGAGGSSASTGFVLNAGITQRQLAMGPGFSLKF

Target ORF information:

RefSeq Version	NM_001259922.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pitslre, transcript variant E (Pitslre), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259922.2

ORF Insert Sequence:

ATGGAGGAGC CAACGCGACG CAGCAGTAAG TACGCGGAGA GCAAGGATGC TGAGAGCCTG 
GAACAGGATC TGCGATCGAG GTTGCTGAAA AAGCGCCATA ACTACGTTAA GGATTATGAA 
ACGGAGGAGA ACTATGAGCA TCGGGTCGAG AGAAGTGATC GCCGGGAGGG AGGACGCAAA 
GAGCGGGAGA GAACTGTGCG AAGTACCCAC AAGCAAAACC GTCACGACCG AGTCATTGAG 
CTGCTGGACA GTCCTGAGCA GGAGCACCAC CATCAGCACC AGCACAAATC GCATCGCAGC 
AAGTGGCGCG AGGAGGTTGA GGTTATCAGA CGAAAGGTGC CCGAGGACTT GGAGCTGTTG 
GCTCGCAGGG AAAAACTTCT TGCCGCTGAA CGGGAAAGCC GCCAGCGAAA ACAGACGGCT 
CGTGAGGAAC TGGAAGCTCG GCGGGAGTTG CTCCGCGAGC GAAACGAGCA CAGTGATGCA 
CTTAGTCCAA CAACAGTGGC TGCAAGTGTA ACCGCAGGTC TGAACATTCA TGTGAAGAGG 
AAGTCCAAGC CGGATAACTA TGAGAAGGAA ATCAAACTCA AGAAGCGGCG GGAAGATGAT 
ATCGAGGTTA TCCGAGATGA TGACGATGAG GAAAGCGAGG AGAGCGACTC AAACGAGGAG 
GTTCCTGAGC AAGACAGCGA AGGAAGCGCA ACAGAGTCTG GAAGCGAAGA CAGCTATGCT 
AGCAAAAAGA AGAGCAAGAT TAAATCCAAG TCTCAATTGG AAGACGATGA CGAGGATTTA 
CCCTTACCCG ACAGTCCACT TAGCGTGGGT GAGCTATACA AGTCGCCCAA GCAACGTCAG 
CGATCTCGCT CTGTAAGCTC CAAGAGCAGC TCCCAGTCCA GTCGAAGCAG TCGGTCCAGA 
TCCCGTTCTA GAAGTCAAAG CAGCCTAGAG GATGAGGTAG ATCGTCAAGA CGTGGGGGCA 
GATGCCTCAC CCAGCAGCAG CACACGCAGC GAGGAACGTG GAATGACCCA AGAGCAGCCG 
GAAGAGAAGC CCGAGGAGAA ACTCAAAGAG AAACAGAAGT CTCTGGAGGA GCAAATTCCG 
TGCGATGACA AGGGCATTCC CCTGCCCAAC TATTATCCCG GTGTGCAGGG CTGCCGGTCT 
GTGGAGGAAT TCCAGTGTCT AAACCGCATC GAAGAGGGAA CCTATGGAGT GGTATATCGA 
GCCAAGGACA AAAGAACCAA TGAGATTGTG GCTCTTAAGC GTCTGAAAAT GGAGAAGGAA 
AAGGAGGGAT TTCCGATAAC ATCACTCAGG GAGATCAACA CTCTTCTCAA GGGTCAGCAC 
CCGAATATTG TGACCGTTCG CGAGATCGTG GTCGGATCCA ACATGGATAA GATATTTATT 
GTGATGGACT ATGTAGAACA CGACCTGAAA TCCCTGATGG AAACGATGAA AAATCGTAAG 
CAGAGCTTTT TCCCCGGAGA AGTCAAATGT CTGACGCAGC AGCTTCTGCG GGCGGTGGCC 
CACCTCCATG ACAATTGGAT ATTGCATCGT GACCTGAAGA CCTCCAATCT CCTCTTGTCC 
CACAAGGGCA TTCTCAAGGT GGGTGATTTC GGATTGGCAA GGGAGTATGG ATCGCCGATC 
AAGAAGTATA CCTCACTTGT TGTCACTCTC TGGTATCGTG CACCCGAGCT CCTTCTCTGC 
TCGCCGGTTT ACTCCACGCC CATAGACGTC TGGTCGGTAG GCTGCATTTT CGCCGAATTC 
TTGCAGATGC TGCCGCTCTT TCCGGGAAAA TCTGAAATCG ATGAGCTTAA CAGGATCTTT 
AAGGAATTGG GAACTCCGAA CGAGAAGATC TGGCCAGGAT ACACCGAGCT CCCAGCCGTG 
AAGAACATGT TGTCGCAGAA CTCTCAGTTT ACCGAGTATC CCGTCTCGCA ACTGCGCAAG 
CACTTCCAGG AGAAGACCTC TGAAATGGGC TTGTCGCTGC TGCAGGGTCT GCTGACGTAC 
GATCCGAAAC AAAGACTGAG CGCCGACGCG GCACTAAAGC ACGGCTTCTT CAAGGAGCTG 
CCATTGCCCA TCGATCCATC CATGTTTCCC ACCTGGCCGG CCAAGAGCGA GTTGGGAGCC 
CGCAAGGCGC AAGCCTCTTC CCCCAAACCG CCATCCGGTG GCTCGCAGTT CAAGCAACTA 
GGCCGCGACG AGCCCATCAT CGTGGGACCG GGAAACAAGC TCTCATCTGG GATTATCACT 
GGCAACAAGA AGAGCCACGG AGCTGGTGGA TCGTCGGCTA GCACGGGATT TGTCCTGAAC 
GCTGGAATAA CGCAGCGCCA GTTGGCCATG GGACCGGGAT TCAGTCTGAA GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pitslre ( NM_001259923.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246852.1 Drosophila melanogaster pitslre, transcript variant F (Pitslre), mRNA.

Price & Availability↑

CloneID	OFa19156
Clone ID Related Accession (Same CDS sequence)	NM_001259922.2 , NM_001259923.2 , NM_001275193.1
Accession Version	NM_001259923.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pitslre, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001259923.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAGC CAACGCGACG CAGCAGTAAG TACGCGGAGA GCAAGGATGC TGAGAGCCTG 
GAACAGGATC TGCGATCGAG GTTGCTGAAA AAGCGCCATA ACTACGTTAA GGATTATGAA 
ACGGAGGAGA ACTATGAGCA TCGGGTCGAG AGAAGTGATC GCCGGGAGGG AGGACGCAAA 
GAGCGGGAGA GAACTGTGCG AAGTACCCAC AAGCAAAACC GTCACGACCG AGTCATTGAG 
CTGCTGGACA GTCCTGAGCA GGAGCACCAC CATCAGCACC AGCACAAATC GCATCGCAGC 
AAGTGGCGCG AGGAGGTTGA GGTTATCAGA CGAAAGGTGC CCGAGGACTT GGAGCTGTTG 
GCTCGCAGGG AAAAACTTCT TGCCGCTGAA CGGGAAAGCC GCCAGCGAAA ACAGACGGCT 
CGTGAGGAAC TGGAAGCTCG GCGGGAGTTG CTCCGCGAGC GAAACGAGCA CAGTGATGCA 
CTTAGTCCAA CAACAGTGGC TGCAAGTGTA ACCGCAGGTC TGAACATTCA TGTGAAGAGG 
AAGTCCAAGC CGGATAACTA TGAGAAGGAA ATCAAACTCA AGAAGCGGCG GGAAGATGAT 
ATCGAGGTTA TCCGAGATGA TGACGATGAG GAAAGCGAGG AGAGCGACTC AAACGAGGAG 
GTTCCTGAGC AAGACAGCGA AGGAAGCGCA ACAGAGTCTG GAAGCGAAGA CAGCTATGCT 
AGCAAAAAGA AGAGCAAGAT TAAATCCAAG TCTCAATTGG AAGACGATGA CGAGGATTTA 
CCCTTACCCG ACAGTCCACT TAGCGTGGGT GAGCTATACA AGTCGCCCAA GCAACGTCAG 
CGATCTCGCT CTGTAAGCTC CAAGAGCAGC TCCCAGTCCA GTCGAAGCAG TCGGTCCAGA 
TCCCGTTCTA GAAGTCAAAG CAGCCTAGAG GATGAGGTAG ATCGTCAAGA CGTGGGGGCA 
GATGCCTCAC CCAGCAGCAG CACACGCAGC GAGGAACGTG GAATGACCCA AGAGCAGCCG 
GAAGAGAAGC CCGAGGAGAA ACTCAAAGAG AAACAGAAGT CTCTGGAGGA GCAAATTCCG 
TGCGATGACA AGGGCATTCC CCTGCCCAAC TATTATCCCG GTGTGCAGGG CTGCCGGTCT 
GTGGAGGAAT TCCAGTGTCT AAACCGCATC GAAGAGGGAA CCTATGGAGT GGTATATCGA 
GCCAAGGACA AAAGAACCAA TGAGATTGTG GCTCTTAAGC GTCTGAAAAT GGAGAAGGAA 
AAGGAGGGAT TTCCGATAAC ATCACTCAGG GAGATCAACA CTCTTCTCAA GGGTCAGCAC 
CCGAATATTG TGACCGTTCG CGAGATCGTG GTCGGATCCA ACATGGATAA GATATTTATT 
GTGATGGACT ATGTAGAACA CGACCTGAAA TCCCTGATGG AAACGATGAA AAATCGTAAG 
CAGAGCTTTT TCCCCGGAGA AGTCAAATGT CTGACGCAGC AGCTTCTGCG GGCGGTGGCC 
CACCTCCATG ACAATTGGAT ATTGCATCGT GACCTGAAGA CCTCCAATCT CCTCTTGTCC 
CACAAGGGCA TTCTCAAGGT GGGTGATTTC GGATTGGCAA GGGAGTATGG ATCGCCGATC 
AAGAAGTATA CCTCACTTGT TGTCACTCTC TGGTATCGTG CACCCGAGCT CCTTCTCTGC 
TCGCCGGTTT ACTCCACGCC CATAGACGTC TGGTCGGTAG GCTGCATTTT CGCCGAATTC 
TTGCAGATGC TGCCGCTCTT TCCGGGAAAA TCTGAAATCG ATGAGCTTAA CAGGATCTTT 
AAGGAATTGG GAACTCCGAA CGAGAAGATC TGGCCAGGAT ACACCGAGCT CCCAGCCGTG 
AAGAACATGT TGTCGCAGAA CTCTCAGTTT ACCGAGTATC CCGTCTCGCA ACTGCGCAAG 
CACTTCCAGG AGAAGACCTC TGAAATGGGC TTGTCGCTGC TGCAGGGTCT GCTGACGTAC 
GATCCGAAAC AAAGACTGAG CGCCGACGCG GCACTAAAGC ACGGCTTCTT CAAGGAGCTG 
CCATTGCCCA TCGATCCATC CATGTTTCCC ACCTGGCCGG CCAAGAGCGA GTTGGGAGCC 
CGCAAGGCGC AAGCCTCTTC CCCCAAACCG CCATCCGGTG GCTCGCAGTT CAAGCAACTA 
GGCCGCGACG AGCCCATCAT CGTGGGACCG GGAAACAAGC TCTCATCTGG GATTATCACT 
GGCAACAAGA AGAGCCACGG AGCTGGTGGA TCGTCGGCTA GCACGGGATT TGTCCTGAAC 
GCTGGAATAA CGCAGCGCCA GTTGGCCATG GGACCGGGAT TCAGTCTGAA GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246852.1
CDS	1154..3490
Translation	MEEPTRRSSKYAESKDAESLEQDLRSRLLKKRHNYVKDYETEENYEHRVERSDRREGGRKERERTVRSTHKQNRHDRVIELLDSPEQEHHHQHQHKSHRSKWREEVEVIRRKVPEDLELLARREKLLAAERESRQRKQTAREELEARRELLRERNEHSDALSPTTVAASVTAGLNIHVKRKSKPDNYEKEIKLKKRREDDIEVIRDDDDEESEESDSNEEVPEQDSEGSATESGSEDSYASKKKSKIKSKSQLEDDDEDLPLPDSPLSVGELYKSPKQRQRSRSVSSKSSSQSSRSSRSRSRSRSQSSLEDEVDRQDVGADASPSSSTRSEERGMTQEQPEEKPEEKLKEKQKSLEEQIPCDDKGIPLPNYYPGVQGCRSVEEFQCLNRIEEGTYGVVYRAKDKRTNEIVALKRLKMEKEKEGFPITSLREINTLLKGQHPNIVTVREIVVGSNMDKIFIVMDYVEHDLKSLMETMKNRKQSFFPGEVKCLTQQLLRAVAHLHDNWILHRDLKTSNLLLSHKGILKVGDFGLAREYGSPIKKYTSLVVTLWYRAPELLLCSPVYSTPIDVWSVGCIFAEFLQMLPLFPGKSEIDELNRIFKELGTPNEKIWPGYTELPAVKNMLSQNSQFTEYPVSQLRKHFQEKTSEMGLSLLQGLLTYDPKQRLSADAALKHGFFKELPLPIDPSMFPTWPAKSELGARKAQASSPKPPSGGSQFKQLGRDEPIIVGPGNKLSSGIITGNKKSHGAGGSSASTGFVLNAGITQRQLAMGPGFSLKF

Target ORF information:

RefSeq Version	NM_001259923.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pitslre, transcript variant F (Pitslre), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259923.2

ORF Insert Sequence:

ATGGAGGAGC CAACGCGACG CAGCAGTAAG TACGCGGAGA GCAAGGATGC TGAGAGCCTG 
GAACAGGATC TGCGATCGAG GTTGCTGAAA AAGCGCCATA ACTACGTTAA GGATTATGAA 
ACGGAGGAGA ACTATGAGCA TCGGGTCGAG AGAAGTGATC GCCGGGAGGG AGGACGCAAA 
GAGCGGGAGA GAACTGTGCG AAGTACCCAC AAGCAAAACC GTCACGACCG AGTCATTGAG 
CTGCTGGACA GTCCTGAGCA GGAGCACCAC CATCAGCACC AGCACAAATC GCATCGCAGC 
AAGTGGCGCG AGGAGGTTGA GGTTATCAGA CGAAAGGTGC CCGAGGACTT GGAGCTGTTG 
GCTCGCAGGG AAAAACTTCT TGCCGCTGAA CGGGAAAGCC GCCAGCGAAA ACAGACGGCT 
CGTGAGGAAC TGGAAGCTCG GCGGGAGTTG CTCCGCGAGC GAAACGAGCA CAGTGATGCA 
CTTAGTCCAA CAACAGTGGC TGCAAGTGTA ACCGCAGGTC TGAACATTCA TGTGAAGAGG 
AAGTCCAAGC CGGATAACTA TGAGAAGGAA ATCAAACTCA AGAAGCGGCG GGAAGATGAT 
ATCGAGGTTA TCCGAGATGA TGACGATGAG GAAAGCGAGG AGAGCGACTC AAACGAGGAG 
GTTCCTGAGC AAGACAGCGA AGGAAGCGCA ACAGAGTCTG GAAGCGAAGA CAGCTATGCT 
AGCAAAAAGA AGAGCAAGAT TAAATCCAAG TCTCAATTGG AAGACGATGA CGAGGATTTA 
CCCTTACCCG ACAGTCCACT TAGCGTGGGT GAGCTATACA AGTCGCCCAA GCAACGTCAG 
CGATCTCGCT CTGTAAGCTC CAAGAGCAGC TCCCAGTCCA GTCGAAGCAG TCGGTCCAGA 
TCCCGTTCTA GAAGTCAAAG CAGCCTAGAG GATGAGGTAG ATCGTCAAGA CGTGGGGGCA 
GATGCCTCAC CCAGCAGCAG CACACGCAGC GAGGAACGTG GAATGACCCA AGAGCAGCCG 
GAAGAGAAGC CCGAGGAGAA ACTCAAAGAG AAACAGAAGT CTCTGGAGGA GCAAATTCCG 
TGCGATGACA AGGGCATTCC CCTGCCCAAC TATTATCCCG GTGTGCAGGG CTGCCGGTCT 
GTGGAGGAAT TCCAGTGTCT AAACCGCATC GAAGAGGGAA CCTATGGAGT GGTATATCGA 
GCCAAGGACA AAAGAACCAA TGAGATTGTG GCTCTTAAGC GTCTGAAAAT GGAGAAGGAA 
AAGGAGGGAT TTCCGATAAC ATCACTCAGG GAGATCAACA CTCTTCTCAA GGGTCAGCAC 
CCGAATATTG TGACCGTTCG CGAGATCGTG GTCGGATCCA ACATGGATAA GATATTTATT 
GTGATGGACT ATGTAGAACA CGACCTGAAA TCCCTGATGG AAACGATGAA AAATCGTAAG 
CAGAGCTTTT TCCCCGGAGA AGTCAAATGT CTGACGCAGC AGCTTCTGCG GGCGGTGGCC 
CACCTCCATG ACAATTGGAT ATTGCATCGT GACCTGAAGA CCTCCAATCT CCTCTTGTCC 
CACAAGGGCA TTCTCAAGGT GGGTGATTTC GGATTGGCAA GGGAGTATGG ATCGCCGATC 
AAGAAGTATA CCTCACTTGT TGTCACTCTC TGGTATCGTG CACCCGAGCT CCTTCTCTGC 
TCGCCGGTTT ACTCCACGCC CATAGACGTC TGGTCGGTAG GCTGCATTTT CGCCGAATTC 
TTGCAGATGC TGCCGCTCTT TCCGGGAAAA TCTGAAATCG ATGAGCTTAA CAGGATCTTT 
AAGGAATTGG GAACTCCGAA CGAGAAGATC TGGCCAGGAT ACACCGAGCT CCCAGCCGTG 
AAGAACATGT TGTCGCAGAA CTCTCAGTTT ACCGAGTATC CCGTCTCGCA ACTGCGCAAG 
CACTTCCAGG AGAAGACCTC TGAAATGGGC TTGTCGCTGC TGCAGGGTCT GCTGACGTAC 
GATCCGAAAC AAAGACTGAG CGCCGACGCG GCACTAAAGC ACGGCTTCTT CAAGGAGCTG 
CCATTGCCCA TCGATCCATC CATGTTTCCC ACCTGGCCGG CCAAGAGCGA GTTGGGAGCC 
CGCAAGGCGC AAGCCTCTTC CCCCAAACCG CCATCCGGTG GCTCGCAGTT CAAGCAACTA 
GGCCGCGACG AGCCCATCAT CGTGGGACCG GGAAACAAGC TCTCATCTGG GATTATCACT 
GGCAACAAGA AGAGCCACGG AGCTGGTGGA TCGTCGGCTA GCACGGGATT TGTCCTGAAC 
GCTGGAATAA CGCAGCGCCA GTTGGCCATG GGACCGGGAT TCAGTCTGAA GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pitslre ( NM_001275193.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262122.1 Drosophila melanogaster pitslre, transcript variant G (Pitslre), mRNA.

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CloneID	OFa19156
Clone ID Related Accession (Same CDS sequence)	NM_001259922.2 , NM_001259923.2 , NM_001275193.1
Accession Version	NM_001275193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pitslre, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAGC CAACGCGACG CAGCAGTAAG TACGCGGAGA GCAAGGATGC TGAGAGCCTG 
GAACAGGATC TGCGATCGAG GTTGCTGAAA AAGCGCCATA ACTACGTTAA GGATTATGAA 
ACGGAGGAGA ACTATGAGCA TCGGGTCGAG AGAAGTGATC GCCGGGAGGG AGGACGCAAA 
GAGCGGGAGA GAACTGTGCG AAGTACCCAC AAGCAAAACC GTCACGACCG AGTCATTGAG 
CTGCTGGACA GTCCTGAGCA GGAGCACCAC CATCAGCACC AGCACAAATC GCATCGCAGC 
AAGTGGCGCG AGGAGGTTGA GGTTATCAGA CGAAAGGTGC CCGAGGACTT GGAGCTGTTG 
GCTCGCAGGG AAAAACTTCT TGCCGCTGAA CGGGAAAGCC GCCAGCGAAA ACAGACGGCT 
CGTGAGGAAC TGGAAGCTCG GCGGGAGTTG CTCCGCGAGC GAAACGAGCA CAGTGATGCA 
CTTAGTCCAA CAACAGTGGC TGCAAGTGTA ACCGCAGGTC TGAACATTCA TGTGAAGAGG 
AAGTCCAAGC CGGATAACTA TGAGAAGGAA ATCAAACTCA AGAAGCGGCG GGAAGATGAT 
ATCGAGGTTA TCCGAGATGA TGACGATGAG GAAAGCGAGG AGAGCGACTC AAACGAGGAG 
GTTCCTGAGC AAGACAGCGA AGGAAGCGCA ACAGAGTCTG GAAGCGAAGA CAGCTATGCT 
AGCAAAAAGA AGAGCAAGAT TAAATCCAAG TCTCAATTGG AAGACGATGA CGAGGATTTA 
CCCTTACCCG ACAGTCCACT TAGCGTGGGT GAGCTATACA AGTCGCCCAA GCAACGTCAG 
CGATCTCGCT CTGTAAGCTC CAAGAGCAGC TCCCAGTCCA GTCGAAGCAG TCGGTCCAGA 
TCCCGTTCTA GAAGTCAAAG CAGCCTAGAG GATGAGGTAG ATCGTCAAGA CGTGGGGGCA 
GATGCCTCAC CCAGCAGCAG CACACGCAGC GAGGAACGTG GAATGACCCA AGAGCAGCCG 
GAAGAGAAGC CCGAGGAGAA ACTCAAAGAG AAACAGAAGT CTCTGGAGGA GCAAATTCCG 
TGCGATGACA AGGGCATTCC CCTGCCCAAC TATTATCCCG GTGTGCAGGG CTGCCGGTCT 
GTGGAGGAAT TCCAGTGTCT AAACCGCATC GAAGAGGGAA CCTATGGAGT GGTATATCGA 
GCCAAGGACA AAAGAACCAA TGAGATTGTG GCTCTTAAGC GTCTGAAAAT GGAGAAGGAA 
AAGGAGGGAT TTCCGATAAC ATCACTCAGG GAGATCAACA CTCTTCTCAA GGGTCAGCAC 
CCGAATATTG TGACCGTTCG CGAGATCGTG GTCGGATCCA ACATGGATAA GATATTTATT 
GTGATGGACT ATGTAGAACA CGACCTGAAA TCCCTGATGG AAACGATGAA AAATCGTAAG 
CAGAGCTTTT TCCCCGGAGA AGTCAAATGT CTGACGCAGC AGCTTCTGCG GGCGGTGGCC 
CACCTCCATG ACAATTGGAT ATTGCATCGT GACCTGAAGA CCTCCAATCT CCTCTTGTCC 
CACAAGGGCA TTCTCAAGGT GGGTGATTTC GGATTGGCAA GGGAGTATGG ATCGCCGATC 
AAGAAGTATA CCTCACTTGT TGTCACTCTC TGGTATCGTG CACCCGAGCT CCTTCTCTGC 
TCGCCGGTTT ACTCCACGCC CATAGACGTC TGGTCGGTAG GCTGCATTTT CGCCGAATTC 
TTGCAGATGC TGCCGCTCTT TCCGGGAAAA TCTGAAATCG ATGAGCTTAA CAGGATCTTT 
AAGGAATTGG GAACTCCGAA CGAGAAGATC TGGCCAGGAT ACACCGAGCT CCCAGCCGTG 
AAGAACATGT TGTCGCAGAA CTCTCAGTTT ACCGAGTATC CCGTCTCGCA ACTGCGCAAG 
CACTTCCAGG AGAAGACCTC TGAAATGGGC TTGTCGCTGC TGCAGGGTCT GCTGACGTAC 
GATCCGAAAC AAAGACTGAG CGCCGACGCG GCACTAAAGC ACGGCTTCTT CAAGGAGCTG 
CCATTGCCCA TCGATCCATC CATGTTTCCC ACCTGGCCGG CCAAGAGCGA GTTGGGAGCC 
CGCAAGGCGC AAGCCTCTTC CCCCAAACCG CCATCCGGTG GCTCGCAGTT CAAGCAACTA 
GGCCGCGACG AGCCCATCAT CGTGGGACCG GGAAACAAGC TCTCATCTGG GATTATCACT 
GGCAACAAGA AGAGCCACGG AGCTGGTGGA TCGTCGGCTA GCACGGGATT TGTCCTGAAC 
GCTGGAATAA CGCAGCGCCA GTTGGCCATG GGACCGGGAT TCAGTCTGAA GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262122.1
CDS	1473..3809
Translation	MEEPTRRSSKYAESKDAESLEQDLRSRLLKKRHNYVKDYETEENYEHRVERSDRREGGRKERERTVRSTHKQNRHDRVIELLDSPEQEHHHQHQHKSHRSKWREEVEVIRRKVPEDLELLARREKLLAAERESRQRKQTAREELEARRELLRERNEHSDALSPTTVAASVTAGLNIHVKRKSKPDNYEKEIKLKKRREDDIEVIRDDDDEESEESDSNEEVPEQDSEGSATESGSEDSYASKKKSKIKSKSQLEDDDEDLPLPDSPLSVGELYKSPKQRQRSRSVSSKSSSQSSRSSRSRSRSRSQSSLEDEVDRQDVGADASPSSSTRSEERGMTQEQPEEKPEEKLKEKQKSLEEQIPCDDKGIPLPNYYPGVQGCRSVEEFQCLNRIEEGTYGVVYRAKDKRTNEIVALKRLKMEKEKEGFPITSLREINTLLKGQHPNIVTVREIVVGSNMDKIFIVMDYVEHDLKSLMETMKNRKQSFFPGEVKCLTQQLLRAVAHLHDNWILHRDLKTSNLLLSHKGILKVGDFGLAREYGSPIKKYTSLVVTLWYRAPELLLCSPVYSTPIDVWSVGCIFAEFLQMLPLFPGKSEIDELNRIFKELGTPNEKIWPGYTELPAVKNMLSQNSQFTEYPVSQLRKHFQEKTSEMGLSLLQGLLTYDPKQRLSADAALKHGFFKELPLPIDPSMFPTWPAKSELGARKAQASSPKPPSGGSQFKQLGRDEPIIVGPGNKLSSGIITGNKKSHGAGGSSASTGFVLNAGITQRQLAMGPGFSLKF

Target ORF information:

RefSeq Version	NM_001275193.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pitslre, transcript variant G (Pitslre), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275193.1

ORF Insert Sequence:

ATGGAGGAGC CAACGCGACG CAGCAGTAAG TACGCGGAGA GCAAGGATGC TGAGAGCCTG 
GAACAGGATC TGCGATCGAG GTTGCTGAAA AAGCGCCATA ACTACGTTAA GGATTATGAA 
ACGGAGGAGA ACTATGAGCA TCGGGTCGAG AGAAGTGATC GCCGGGAGGG AGGACGCAAA 
GAGCGGGAGA GAACTGTGCG AAGTACCCAC AAGCAAAACC GTCACGACCG AGTCATTGAG 
CTGCTGGACA GTCCTGAGCA GGAGCACCAC CATCAGCACC AGCACAAATC GCATCGCAGC 
AAGTGGCGCG AGGAGGTTGA GGTTATCAGA CGAAAGGTGC CCGAGGACTT GGAGCTGTTG 
GCTCGCAGGG AAAAACTTCT TGCCGCTGAA CGGGAAAGCC GCCAGCGAAA ACAGACGGCT 
CGTGAGGAAC TGGAAGCTCG GCGGGAGTTG CTCCGCGAGC GAAACGAGCA CAGTGATGCA 
CTTAGTCCAA CAACAGTGGC TGCAAGTGTA ACCGCAGGTC TGAACATTCA TGTGAAGAGG 
AAGTCCAAGC CGGATAACTA TGAGAAGGAA ATCAAACTCA AGAAGCGGCG GGAAGATGAT 
ATCGAGGTTA TCCGAGATGA TGACGATGAG GAAAGCGAGG AGAGCGACTC AAACGAGGAG 
GTTCCTGAGC AAGACAGCGA AGGAAGCGCA ACAGAGTCTG GAAGCGAAGA CAGCTATGCT 
AGCAAAAAGA AGAGCAAGAT TAAATCCAAG TCTCAATTGG AAGACGATGA CGAGGATTTA 
CCCTTACCCG ACAGTCCACT TAGCGTGGGT GAGCTATACA AGTCGCCCAA GCAACGTCAG 
CGATCTCGCT CTGTAAGCTC CAAGAGCAGC TCCCAGTCCA GTCGAAGCAG TCGGTCCAGA 
TCCCGTTCTA GAAGTCAAAG CAGCCTAGAG GATGAGGTAG ATCGTCAAGA CGTGGGGGCA 
GATGCCTCAC CCAGCAGCAG CACACGCAGC GAGGAACGTG GAATGACCCA AGAGCAGCCG 
GAAGAGAAGC CCGAGGAGAA ACTCAAAGAG AAACAGAAGT CTCTGGAGGA GCAAATTCCG 
TGCGATGACA AGGGCATTCC CCTGCCCAAC TATTATCCCG GTGTGCAGGG CTGCCGGTCT 
GTGGAGGAAT TCCAGTGTCT AAACCGCATC GAAGAGGGAA CCTATGGAGT GGTATATCGA 
GCCAAGGACA AAAGAACCAA TGAGATTGTG GCTCTTAAGC GTCTGAAAAT GGAGAAGGAA 
AAGGAGGGAT TTCCGATAAC ATCACTCAGG GAGATCAACA CTCTTCTCAA GGGTCAGCAC 
CCGAATATTG TGACCGTTCG CGAGATCGTG GTCGGATCCA ACATGGATAA GATATTTATT 
GTGATGGACT ATGTAGAACA CGACCTGAAA TCCCTGATGG AAACGATGAA AAATCGTAAG 
CAGAGCTTTT TCCCCGGAGA AGTCAAATGT CTGACGCAGC AGCTTCTGCG GGCGGTGGCC 
CACCTCCATG ACAATTGGAT ATTGCATCGT GACCTGAAGA CCTCCAATCT CCTCTTGTCC 
CACAAGGGCA TTCTCAAGGT GGGTGATTTC GGATTGGCAA GGGAGTATGG ATCGCCGATC 
AAGAAGTATA CCTCACTTGT TGTCACTCTC TGGTATCGTG CACCCGAGCT CCTTCTCTGC 
TCGCCGGTTT ACTCCACGCC CATAGACGTC TGGTCGGTAG GCTGCATTTT CGCCGAATTC 
TTGCAGATGC TGCCGCTCTT TCCGGGAAAA TCTGAAATCG ATGAGCTTAA CAGGATCTTT 
AAGGAATTGG GAACTCCGAA CGAGAAGATC TGGCCAGGAT ACACCGAGCT CCCAGCCGTG 
AAGAACATGT TGTCGCAGAA CTCTCAGTTT ACCGAGTATC CCGTCTCGCA ACTGCGCAAG 
CACTTCCAGG AGAAGACCTC TGAAATGGGC TTGTCGCTGC TGCAGGGTCT GCTGACGTAC 
GATCCGAAAC AAAGACTGAG CGCCGACGCG GCACTAAAGC ACGGCTTCTT CAAGGAGCTG 
CCATTGCCCA TCGATCCATC CATGTTTCCC ACCTGGCCGG CCAAGAGCGA GTTGGGAGCC 
CGCAAGGCGC AAGCCTCTTC CCCCAAACCG CCATCCGGTG GCTCGCAGTT CAAGCAACTA 
GGCCGCGACG AGCCCATCAT CGTGGGACCG GGAAACAAGC TCTCATCTGG GATTATCACT 
GGCAACAAGA AGAGCCACGG AGCTGGTGGA TCGTCGGCTA GCACGGGATT TGTCCTGAAC 
GCTGGAATAA CGCAGCGCCA GTTGGCCATG GGACCGGGAT TCAGTCTGAA GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pitslre cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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