Toll-9 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Toll-9
Entrez Gene ID	40245
Full Name	CG5528 gene product from transcript CG5528-RA
Synonyms	CG5528,CT17508,Dm Toll-9,Dmel\CG5528,TOLL 9,Tak/Toll-like,Tl-5,Tl-9,Toll 9,dTLR9,dToll9,toll
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 77B6-77B6

mRNA and Protein(s)

mRNA	Protein	Name
NM_001259916.2	NP_001246845.1	Toll-9, isoform B
NM_140957.4	NP_649214.1	Toll-9, isoform A
NM_001259917.1	NP_001246846.1	Toll-9, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Toll-9	NP_001246845.1
Anopheles gambiae (African malaria mosquito)	TOLL9	XP_565221.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Toll-9 is neither required to maintain a basal anti-microbial response nor to mount an efficient immune response to bacterial infection.
Title: Lack of an antibacterial response defect in Drosophila Toll-9 mutant.

results suggest that Toll-9 is a constitutively active protein, and implies its novel function in protecting the host by maintaining a substantial level of antimicrobial gene products to ward off the continuous challenge of microorganisms
Title: The Drosophila Toll-9 activates a constitutive antimicrobial defense.

The following Toll-9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Toll-9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19101	NM_140957.4 Latest version!	Drosophila melanogaster Toll-9, transcript variant A (Toll-9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa19101	NM_001259917.1 Latest version!	Drosophila melanogaster Toll-9, transcript variant C (Toll-9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa21128	NM_001259916.2 Latest version!	Drosophila melanogaster Toll-9, transcript variant B (Toll-9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Toll-9 ( NM_140957.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649214.1 Drosophila melanogaster Toll-9, transcript variant A (Toll-9), mRNA.

Price & Availability↑

CloneID	OFa19101
Clone ID Related Accession (Same CDS sequence)	NM_140957.4 , NM_001259917.1
Accession Version	NM_140957.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Toll-9, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_140957.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGTCCAA AATATATATG GGATGTGATC GTATTAGTAT GCCTGTTCCT TGGAAACGTA 
AGGGAAGCAT ACACCGAGTT TTCGATCCAG GATGGCTTGA TAATAGAACC AGATTCAGCC 
ACAACATCCA GCGAAGAGGC CGAAGAGGTC AGCAAGGAGA GAACGGACCT CAAATCCCTC 
ATGTTGAAGT ACGAATCGGA TGATGGGAAC AGTTGTCTGC TGGATCTGAT CAAGGACGAG 
GTGATATGGT GGCAGTTTCC CAACGGAACC CTCAGGGACA GCACAAAGAA ATACGCGCAC 
AAATTGTATT TGGATCTATC CCACGGTAAT CTTAAGGACG ACTCTGACCT ATTCCGAGAA 
GCGAAACTTT CGAGGAAGGT TACCATTTGG AGGACAGAAG TCTTCTCGGC CGCATTCAAT 
ACGTTGACTG CAGCTCCGTT CAGGACGCTC TACTCCATGA GGGAATCCTT GAAGCTTTTA 
TCGCTGCGAG GAAATAACTT TGCTGAACTG ATTCCAGATG CTGAAGACTT TGCTCGATTT 
GTAAACGAGT CCCGCTTGGA GGCTAGTAAC TCGGTACCAC ACCATTGTGA ACTACTACTT 
TTACACAATA CCACCGATCT GTATGATCGG GAATGCTATC TATACTTTAA CAACAACACA 
AACATGGGAC AATCAATCAC AACCGGTAGA AACTATACGA ACTTTATTAA GGTCTTAAAA 
GATCGGTTCG ATCAGCACGG AAGTTCATCC CAATCTATAG CCTGGGCCAC TTTTCCAAAG 
ATGCCCCGAC TTGTGGAGCT GGACATCAGC AACTGCAGCA TCGAGTACGT GAGCAAGGAG 
GCGTTCCGGA ATGTCAGCAA TCTGCGGAGG CTCTTCATGT CGGACAACAA GATCATGACG 
ATAAGCCACG ACACTTTCTA CTACGTCCAG GGAGTGCAGT ATCTCGACCT GTCCTTCACT 
AACTTCCTGA CCTATAGCTA CCAACTGCAG TTGCCCACTC TGGAAATGGC CTTAAGCCTG 
ATATACGGCC TGAAGATCCA GCAGAACGTG TTCAAATATC TGCCGGAACT GATTTACCTG 
GACCTCTCCC ACTCGAAAAT GACCAGGAAT AGTGCGGTGG CTTTTGCCCA TCTGGGGGAT 
AAGCTGAAGT TCCTCAGTCT GTGCTACACT GCCATTCCCA TGGTGAGCAG TACCATCTTC 
AAGAATACGG TTCTGGAGGG CCTCGACTTG TCCGGAAACC CCTACCTATC CTACAACATC 
ATCGACGACG CCTTCGATGG CATCGCTAAC ACTCTGAAGT ATTTGTACTT CGAGCGATCG 
AATATTAAGG ATTTAGAGTG GTCCAAGTCG TTGAAGAACC TTCAGGTGTT GGGCCTCGCG 
GGTAACAACA TCAACGCTCT CACTCCCGCC ATGTTTCAGT CTCTGGAGTC CCTGGAGATC 
CTCGACTTGA GCTCGAACCA TGTGGGCAAT TGGTACCGCA GTGCCTTCCA CAACAACTCC 
GCCCTAAGGG TTTTGAACCT GAGAAGCAAC ACCATCAACA TGCTGTCCAA CGAGATGTTG 
AAGGACTTTG AGCGATTGGA TTACCTTAGT CTCGGGGACA ACGACTTTAT TTGCGACTGC 
CATTTGAGAG CGGTGGTGGA GGTGGCGGCC GCCAATAATA AAGATGCAGA CTGCTCCTAT 
AGACTGCTGA ACTATAGTCA GAATGCCGTA GGTGAGGAGG TAATATCTCT GGCGGAGTCA 
CTAATTATAG ATCGCAAGCT ATGGCAGAGT CGCTATATTC CCTGGCTGCA GAGAAGTTAC 
TCCAACATCA GGGAGTTCAA TCGCGCGAAT CATATAATCA AGCTGCGCTT CTCCTCCGAG 
GACTACATGG TAGCCAAGTG CTCCGCGGCC CAGCCATACC ACTTAGGGGA CCTTGACGGA 
GATCTTACCC TAAAATTTCA GCTGCTCGAC TACGAGGCCA GTCAGTATTA CTGCTTCAAC 
AACACCGATC AGCTCCAGGT GGATGAGCTC AACTGCCAGA TCAGGTCGAT GAGTGACCTC 
GCCGAGGAGT TGCACCACGT TACGAACACA GTGATCGCAG TCATGGGCAG CTTAGTTGGC 
GCTTGCATCC TGGGATTTAT AATTTATCTG AAGCGCTGGC ACATCCACTA CTATTACTCT 
TCCCTGAAGT CCGCTGCCTT GTTGTCCAGT GCGTCCAAGG AGTCGGTGAA TAAATTCACA 
AACATATCCC AGAGAGATCC CAGTGCCGTC TACGATATCT TCATCAGCTA CTGCCAGAAT 
GATCGGACCT GGGTGCTCAA TGAACTGCTG CCGAACGTGG AGGAAACTGG GGATGTGTCC 
ATTTGTCTGC ATGAAAGAGA TTTTCAGATT GGAGTGACCA TCCTAGATAA CATCATCTCC 
TGCATGGATC GCTCGTATTC CCTTATGCTG ATCATATCCT CCAAGTTCCT GTTGAGTCAT 
TGGTGTCAAT TTGAAATGTA TTTGGCACAG CATCGCATCT TTGAGGTCAG CAAGGAGCAC 
TTGATCCTGG TTTTTCTGGA GGACATTCCA CGGCGAAAGC GACCCAAGAC TTTGCAATAT 
CTCATGGATG TGAAAACGTA TATAAAGTGG CCGACGGCCA AGGAGGATCG AAAGCTGTTT 
TGGAAACGTC TGAAGAGATC CCTGGAGGTC ATAGGGATCA ACTCCAGAGA GATCAGTGTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649214.1
CDS	591..3293
Translation	MCPKYIWDVIVLVCLFLGNVREAYTEFSIQDGLIIEPDSATTSSEEAEEVSKERTDLKSLMLKYESDDGNSCLLDLIKDEVIWWQFPNGTLRDSTKKYAHKLYLDLSHGNLKDDSDLFREAKLSRKVTIWRTEVFSAAFNTLTAAPFRTLYSMRESLKLLSLRGNNFAELIPDAEDFARFVNESRLEASNSVPHHCELLLLHNTTDLYDRECYLYFNNNTNMGQSITTGRNYTNFIKVLKDRFDQHGSSSQSIAWATFPKMPRLVELDISNCSIEYVSKEAFRNVSNLRRLFMSDNKIMTISHDTFYYVQGVQYLDLSFTNFLTYSYQLQLPTLEMALSLIYGLKIQQNVFKYLPELIYLDLSHSKMTRNSAVAFAHLGDKLKFLSLCYTAIPMVSSTIFKNTVLEGLDLSGNPYLSYNIIDDAFDGIANTLKYLYFERSNIKDLEWSKSLKNLQVLGLAGNNINALTPAMFQSLESLEILDLSSNHVGNWYRSAFHNNSALRVLNLRSNTINMLSNEMLKDFERLDYLSLGDNDFICDCHLRAVVEVAAANNKDADCSYRLLNYSQNAVGEEVISLAESLIIDRKLWQSRYIPWLQRSYSNIREFNRANHIIKLRFSSEDYMVAKCSAAQPYHLGDLDGDLTLKFQLLDYEASQYYCFNNTDQLQVDELNCQIRSMSDLAEELHHVTNTVIAVMGSLVGACILGFIIYLKRWHIHYYYSSLKSAALLSSASKESVNKFTNISQRDPSAVYDIFISYCQNDRTWVLNELLPNVEETGDVSICLHERDFQIGVTILDNIISCMDRSYSLMLIISSKFLLSHWCQFEMYLAQHRIFEVSKEHLILVFLEDIPRRKRPKTLQYLMDVKTYIKWPTAKEDRKLFWKRLKRSLEVIGINSREISV

Target ORF information:

RefSeq Version	NM_140957.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Toll-9, transcript variant A (Toll-9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140957.4

ORF Insert Sequence:

ATGTGTCCAA AATATATATG GGATGTGATC GTATTAGTAT GCCTGTTCCT TGGAAACGTA 
AGGGAAGCAT ACACCGAGTT TTCGATCCAG GATGGCTTGA TAATAGAACC AGATTCAGCC 
ACAACATCCA GCGAAGAGGC CGAAGAGGTC AGCAAGGAGA GAACGGACCT CAAATCCCTC 
ATGTTGAAGT ACGAATCGGA TGATGGGAAC AGTTGTCTGC TGGATCTGAT CAAGGACGAG 
GTGATATGGT GGCAGTTTCC CAACGGAACC CTCAGGGACA GCACAAAGAA ATACGCGCAC 
AAATTGTATT TGGATCTATC CCACGGTAAT CTTAAGGACG ACTCTGACCT ATTCCGAGAA 
GCGAAACTTT CGAGGAAGGT TACCATTTGG AGGACAGAAG TCTTCTCGGC CGCATTCAAT 
ACGTTGACTG CAGCTCCGTT CAGGACGCTC TACTCCATGA GGGAATCCTT GAAGCTTTTA 
TCGCTGCGAG GAAATAACTT TGCTGAACTG ATTCCAGATG CTGAAGACTT TGCTCGATTT 
GTAAACGAGT CCCGCTTGGA GGCTAGTAAC TCGGTACCAC ACCATTGTGA ACTACTACTT 
TTACACAATA CCACCGATCT GTATGATCGG GAATGCTATC TATACTTTAA CAACAACACA 
AACATGGGAC AATCAATCAC AACCGGTAGA AACTATACGA ACTTTATTAA GGTCTTAAAA 
GATCGGTTCG ATCAGCACGG AAGTTCATCC CAATCTATAG CCTGGGCCAC TTTTCCAAAG 
ATGCCCCGAC TTGTGGAGCT GGACATCAGC AACTGCAGCA TCGAGTACGT GAGCAAGGAG 
GCGTTCCGGA ATGTCAGCAA TCTGCGGAGG CTCTTCATGT CGGACAACAA GATCATGACG 
ATAAGCCACG ACACTTTCTA CTACGTCCAG GGAGTGCAGT ATCTCGACCT GTCCTTCACT 
AACTTCCTGA CCTATAGCTA CCAACTGCAG TTGCCCACTC TGGAAATGGC CTTAAGCCTG 
ATATACGGCC TGAAGATCCA GCAGAACGTG TTCAAATATC TGCCGGAACT GATTTACCTG 
GACCTCTCCC ACTCGAAAAT GACCAGGAAT AGTGCGGTGG CTTTTGCCCA TCTGGGGGAT 
AAGCTGAAGT TCCTCAGTCT GTGCTACACT GCCATTCCCA TGGTGAGCAG TACCATCTTC 
AAGAATACGG TTCTGGAGGG CCTCGACTTG TCCGGAAACC CCTACCTATC CTACAACATC 
ATCGACGACG CCTTCGATGG CATCGCTAAC ACTCTGAAGT ATTTGTACTT CGAGCGATCG 
AATATTAAGG ATTTAGAGTG GTCCAAGTCG TTGAAGAACC TTCAGGTGTT GGGCCTCGCG 
GGTAACAACA TCAACGCTCT CACTCCCGCC ATGTTTCAGT CTCTGGAGTC CCTGGAGATC 
CTCGACTTGA GCTCGAACCA TGTGGGCAAT TGGTACCGCA GTGCCTTCCA CAACAACTCC 
GCCCTAAGGG TTTTGAACCT GAGAAGCAAC ACCATCAACA TGCTGTCCAA CGAGATGTTG 
AAGGACTTTG AGCGATTGGA TTACCTTAGT CTCGGGGACA ACGACTTTAT TTGCGACTGC 
CATTTGAGAG CGGTGGTGGA GGTGGCGGCC GCCAATAATA AAGATGCAGA CTGCTCCTAT 
AGACTGCTGA ACTATAGTCA GAATGCCGTA GGTGAGGAGG TAATATCTCT GGCGGAGTCA 
CTAATTATAG ATCGCAAGCT ATGGCAGAGT CGCTATATTC CCTGGCTGCA GAGAAGTTAC 
TCCAACATCA GGGAGTTCAA TCGCGCGAAT CATATAATCA AGCTGCGCTT CTCCTCCGAG 
GACTACATGG TAGCCAAGTG CTCCGCGGCC CAGCCATACC ACTTAGGGGA CCTTGACGGA 
GATCTTACCC TAAAATTTCA GCTGCTCGAC TACGAGGCCA GTCAGTATTA CTGCTTCAAC 
AACACCGATC AGCTCCAGGT GGATGAGCTC AACTGCCAGA TCAGGTCGAT GAGTGACCTC 
GCCGAGGAGT TGCACCACGT TACGAACACA GTGATCGCAG TCATGGGCAG CTTAGTTGGC 
GCTTGCATCC TGGGATTTAT AATTTATCTG AAGCGCTGGC ACATCCACTA CTATTACTCT 
TCCCTGAAGT CCGCTGCCTT GTTGTCCAGT GCGTCCAAGG AGTCGGTGAA TAAATTCACA 
AACATATCCC AGAGAGATCC CAGTGCCGTC TACGATATCT TCATCAGCTA CTGCCAGAAT 
GATCGGACCT GGGTGCTCAA TGAACTGCTG CCGAACGTGG AGGAAACTGG GGATGTGTCC 
ATTTGTCTGC ATGAAAGAGA TTTTCAGATT GGAGTGACCA TCCTAGATAA CATCATCTCC 
TGCATGGATC GCTCGTATTC CCTTATGCTG ATCATATCCT CCAAGTTCCT GTTGAGTCAT 
TGGTGTCAAT TTGAAATGTA TTTGGCACAG CATCGCATCT TTGAGGTCAG CAAGGAGCAC 
TTGATCCTGG TTTTTCTGGA GGACATTCCA CGGCGAAAGC GACCCAAGAC TTTGCAATAT 
CTCATGGATG TGAAAACGTA TATAAAGTGG CCGACGGCCA AGGAGGATCG AAAGCTGTTT 
TGGAAACGTC TGAAGAGATC CCTGGAGGTC ATAGGGATCA ACTCCAGAGA GATCAGTGTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Toll-9 ( NM_001259917.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246846.1 Drosophila melanogaster Toll-9, transcript variant C (Toll-9), mRNA.

Price & Availability↑

CloneID	OFa19101
Clone ID Related Accession (Same CDS sequence)	NM_140957.4 , NM_001259917.1
Accession Version	NM_001259917.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Toll-9, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGTCCAA AATATATATG GGATGTGATC GTATTAGTAT GCCTGTTCCT TGGAAACGTA 
AGGGAAGCAT ACACCGAGTT TTCGATCCAG GATGGCTTGA TAATAGAACC AGATTCAGCC 
ACAACATCCA GCGAAGAGGC CGAAGAGGTC AGCAAGGAGA GAACGGACCT CAAATCCCTC 
ATGTTGAAGT ACGAATCGGA TGATGGGAAC AGTTGTCTGC TGGATCTGAT CAAGGACGAG 
GTGATATGGT GGCAGTTTCC CAACGGAACC CTCAGGGACA GCACAAAGAA ATACGCGCAC 
AAATTGTATT TGGATCTATC CCACGGTAAT CTTAAGGACG ACTCTGACCT ATTCCGAGAA 
GCGAAACTTT CGAGGAAGGT TACCATTTGG AGGACAGAAG TCTTCTCGGC CGCATTCAAT 
ACGTTGACTG CAGCTCCGTT CAGGACGCTC TACTCCATGA GGGAATCCTT GAAGCTTTTA 
TCGCTGCGAG GAAATAACTT TGCTGAACTG ATTCCAGATG CTGAAGACTT TGCTCGATTT 
GTAAACGAGT CCCGCTTGGA GGCTAGTAAC TCGGTACCAC ACCATTGTGA ACTACTACTT 
TTACACAATA CCACCGATCT GTATGATCGG GAATGCTATC TATACTTTAA CAACAACACA 
AACATGGGAC AATCAATCAC AACCGGTAGA AACTATACGA ACTTTATTAA GGTCTTAAAA 
GATCGGTTCG ATCAGCACGG AAGTTCATCC CAATCTATAG CCTGGGCCAC TTTTCCAAAG 
ATGCCCCGAC TTGTGGAGCT GGACATCAGC AACTGCAGCA TCGAGTACGT GAGCAAGGAG 
GCGTTCCGGA ATGTCAGCAA TCTGCGGAGG CTCTTCATGT CGGACAACAA GATCATGACG 
ATAAGCCACG ACACTTTCTA CTACGTCCAG GGAGTGCAGT ATCTCGACCT GTCCTTCACT 
AACTTCCTGA CCTATAGCTA CCAACTGCAG TTGCCCACTC TGGAAATGGC CTTAAGCCTG 
ATATACGGCC TGAAGATCCA GCAGAACGTG TTCAAATATC TGCCGGAACT GATTTACCTG 
GACCTCTCCC ACTCGAAAAT GACCAGGAAT AGTGCGGTGG CTTTTGCCCA TCTGGGGGAT 
AAGCTGAAGT TCCTCAGTCT GTGCTACACT GCCATTCCCA TGGTGAGCAG TACCATCTTC 
AAGAATACGG TTCTGGAGGG CCTCGACTTG TCCGGAAACC CCTACCTATC CTACAACATC 
ATCGACGACG CCTTCGATGG CATCGCTAAC ACTCTGAAGT ATTTGTACTT CGAGCGATCG 
AATATTAAGG ATTTAGAGTG GTCCAAGTCG TTGAAGAACC TTCAGGTGTT GGGCCTCGCG 
GGTAACAACA TCAACGCTCT CACTCCCGCC ATGTTTCAGT CTCTGGAGTC CCTGGAGATC 
CTCGACTTGA GCTCGAACCA TGTGGGCAAT TGGTACCGCA GTGCCTTCCA CAACAACTCC 
GCCCTAAGGG TTTTGAACCT GAGAAGCAAC ACCATCAACA TGCTGTCCAA CGAGATGTTG 
AAGGACTTTG AGCGATTGGA TTACCTTAGT CTCGGGGACA ACGACTTTAT TTGCGACTGC 
CATTTGAGAG CGGTGGTGGA GGTGGCGGCC GCCAATAATA AAGATGCAGA CTGCTCCTAT 
AGACTGCTGA ACTATAGTCA GAATGCCGTA GGTGAGGAGG TAATATCTCT GGCGGAGTCA 
CTAATTATAG ATCGCAAGCT ATGGCAGAGT CGCTATATTC CCTGGCTGCA GAGAAGTTAC 
TCCAACATCA GGGAGTTCAA TCGCGCGAAT CATATAATCA AGCTGCGCTT CTCCTCCGAG 
GACTACATGG TAGCCAAGTG CTCCGCGGCC CAGCCATACC ACTTAGGGGA CCTTGACGGA 
GATCTTACCC TAAAATTTCA GCTGCTCGAC TACGAGGCCA GTCAGTATTA CTGCTTCAAC 
AACACCGATC AGCTCCAGGT GGATGAGCTC AACTGCCAGA TCAGGTCGAT GAGTGACCTC 
GCCGAGGAGT TGCACCACGT TACGAACACA GTGATCGCAG TCATGGGCAG CTTAGTTGGC 
GCTTGCATCC TGGGATTTAT AATTTATCTG AAGCGCTGGC ACATCCACTA CTATTACTCT 
TCCCTGAAGT CCGCTGCCTT GTTGTCCAGT GCGTCCAAGG AGTCGGTGAA TAAATTCACA 
AACATATCCC AGAGAGATCC CAGTGCCGTC TACGATATCT TCATCAGCTA CTGCCAGAAT 
GATCGGACCT GGGTGCTCAA TGAACTGCTG CCGAACGTGG AGGAAACTGG GGATGTGTCC 
ATTTGTCTGC ATGAAAGAGA TTTTCAGATT GGAGTGACCA TCCTAGATAA CATCATCTCC 
TGCATGGATC GCTCGTATTC CCTTATGCTG ATCATATCCT CCAAGTTCCT GTTGAGTCAT 
TGGTGTCAAT TTGAAATGTA TTTGGCACAG CATCGCATCT TTGAGGTCAG CAAGGAGCAC 
TTGATCCTGG TTTTTCTGGA GGACATTCCA CGGCGAAAGC GACCCAAGAC TTTGCAATAT 
CTCATGGATG TGAAAACGTA TATAAAGTGG CCGACGGCCA AGGAGGATCG AAAGCTGTTT 
TGGAAACGTC TGAAGAGATC CCTGGAGGTC ATAGGGATCA ACTCCAGAGA GATCAGTGTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246846.1
CDS	6..2708
Translation	MCPKYIWDVIVLVCLFLGNVREAYTEFSIQDGLIIEPDSATTSSEEAEEVSKERTDLKSLMLKYESDDGNSCLLDLIKDEVIWWQFPNGTLRDSTKKYAHKLYLDLSHGNLKDDSDLFREAKLSRKVTIWRTEVFSAAFNTLTAAPFRTLYSMRESLKLLSLRGNNFAELIPDAEDFARFVNESRLEASNSVPHHCELLLLHNTTDLYDRECYLYFNNNTNMGQSITTGRNYTNFIKVLKDRFDQHGSSSQSIAWATFPKMPRLVELDISNCSIEYVSKEAFRNVSNLRRLFMSDNKIMTISHDTFYYVQGVQYLDLSFTNFLTYSYQLQLPTLEMALSLIYGLKIQQNVFKYLPELIYLDLSHSKMTRNSAVAFAHLGDKLKFLSLCYTAIPMVSSTIFKNTVLEGLDLSGNPYLSYNIIDDAFDGIANTLKYLYFERSNIKDLEWSKSLKNLQVLGLAGNNINALTPAMFQSLESLEILDLSSNHVGNWYRSAFHNNSALRVLNLRSNTINMLSNEMLKDFERLDYLSLGDNDFICDCHLRAVVEVAAANNKDADCSYRLLNYSQNAVGEEVISLAESLIIDRKLWQSRYIPWLQRSYSNIREFNRANHIIKLRFSSEDYMVAKCSAAQPYHLGDLDGDLTLKFQLLDYEASQYYCFNNTDQLQVDELNCQIRSMSDLAEELHHVTNTVIAVMGSLVGACILGFIIYLKRWHIHYYYSSLKSAALLSSASKESVNKFTNISQRDPSAVYDIFISYCQNDRTWVLNELLPNVEETGDVSICLHERDFQIGVTILDNIISCMDRSYSLMLIISSKFLLSHWCQFEMYLAQHRIFEVSKEHLILVFLEDIPRRKRPKTLQYLMDVKTYIKWPTAKEDRKLFWKRLKRSLEVIGINSREISV

Target ORF information:

RefSeq Version	NM_001259917.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Toll-9, transcript variant C (Toll-9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259917.1

ORF Insert Sequence:

ATGTGTCCAA AATATATATG GGATGTGATC GTATTAGTAT GCCTGTTCCT TGGAAACGTA 
AGGGAAGCAT ACACCGAGTT TTCGATCCAG GATGGCTTGA TAATAGAACC AGATTCAGCC 
ACAACATCCA GCGAAGAGGC CGAAGAGGTC AGCAAGGAGA GAACGGACCT CAAATCCCTC 
ATGTTGAAGT ACGAATCGGA TGATGGGAAC AGTTGTCTGC TGGATCTGAT CAAGGACGAG 
GTGATATGGT GGCAGTTTCC CAACGGAACC CTCAGGGACA GCACAAAGAA ATACGCGCAC 
AAATTGTATT TGGATCTATC CCACGGTAAT CTTAAGGACG ACTCTGACCT ATTCCGAGAA 
GCGAAACTTT CGAGGAAGGT TACCATTTGG AGGACAGAAG TCTTCTCGGC CGCATTCAAT 
ACGTTGACTG CAGCTCCGTT CAGGACGCTC TACTCCATGA GGGAATCCTT GAAGCTTTTA 
TCGCTGCGAG GAAATAACTT TGCTGAACTG ATTCCAGATG CTGAAGACTT TGCTCGATTT 
GTAAACGAGT CCCGCTTGGA GGCTAGTAAC TCGGTACCAC ACCATTGTGA ACTACTACTT 
TTACACAATA CCACCGATCT GTATGATCGG GAATGCTATC TATACTTTAA CAACAACACA 
AACATGGGAC AATCAATCAC AACCGGTAGA AACTATACGA ACTTTATTAA GGTCTTAAAA 
GATCGGTTCG ATCAGCACGG AAGTTCATCC CAATCTATAG CCTGGGCCAC TTTTCCAAAG 
ATGCCCCGAC TTGTGGAGCT GGACATCAGC AACTGCAGCA TCGAGTACGT GAGCAAGGAG 
GCGTTCCGGA ATGTCAGCAA TCTGCGGAGG CTCTTCATGT CGGACAACAA GATCATGACG 
ATAAGCCACG ACACTTTCTA CTACGTCCAG GGAGTGCAGT ATCTCGACCT GTCCTTCACT 
AACTTCCTGA CCTATAGCTA CCAACTGCAG TTGCCCACTC TGGAAATGGC CTTAAGCCTG 
ATATACGGCC TGAAGATCCA GCAGAACGTG TTCAAATATC TGCCGGAACT GATTTACCTG 
GACCTCTCCC ACTCGAAAAT GACCAGGAAT AGTGCGGTGG CTTTTGCCCA TCTGGGGGAT 
AAGCTGAAGT TCCTCAGTCT GTGCTACACT GCCATTCCCA TGGTGAGCAG TACCATCTTC 
AAGAATACGG TTCTGGAGGG CCTCGACTTG TCCGGAAACC CCTACCTATC CTACAACATC 
ATCGACGACG CCTTCGATGG CATCGCTAAC ACTCTGAAGT ATTTGTACTT CGAGCGATCG 
AATATTAAGG ATTTAGAGTG GTCCAAGTCG TTGAAGAACC TTCAGGTGTT GGGCCTCGCG 
GGTAACAACA TCAACGCTCT CACTCCCGCC ATGTTTCAGT CTCTGGAGTC CCTGGAGATC 
CTCGACTTGA GCTCGAACCA TGTGGGCAAT TGGTACCGCA GTGCCTTCCA CAACAACTCC 
GCCCTAAGGG TTTTGAACCT GAGAAGCAAC ACCATCAACA TGCTGTCCAA CGAGATGTTG 
AAGGACTTTG AGCGATTGGA TTACCTTAGT CTCGGGGACA ACGACTTTAT TTGCGACTGC 
CATTTGAGAG CGGTGGTGGA GGTGGCGGCC GCCAATAATA AAGATGCAGA CTGCTCCTAT 
AGACTGCTGA ACTATAGTCA GAATGCCGTA GGTGAGGAGG TAATATCTCT GGCGGAGTCA 
CTAATTATAG ATCGCAAGCT ATGGCAGAGT CGCTATATTC CCTGGCTGCA GAGAAGTTAC 
TCCAACATCA GGGAGTTCAA TCGCGCGAAT CATATAATCA AGCTGCGCTT CTCCTCCGAG 
GACTACATGG TAGCCAAGTG CTCCGCGGCC CAGCCATACC ACTTAGGGGA CCTTGACGGA 
GATCTTACCC TAAAATTTCA GCTGCTCGAC TACGAGGCCA GTCAGTATTA CTGCTTCAAC 
AACACCGATC AGCTCCAGGT GGATGAGCTC AACTGCCAGA TCAGGTCGAT GAGTGACCTC 
GCCGAGGAGT TGCACCACGT TACGAACACA GTGATCGCAG TCATGGGCAG CTTAGTTGGC 
GCTTGCATCC TGGGATTTAT AATTTATCTG AAGCGCTGGC ACATCCACTA CTATTACTCT 
TCCCTGAAGT CCGCTGCCTT GTTGTCCAGT GCGTCCAAGG AGTCGGTGAA TAAATTCACA 
AACATATCCC AGAGAGATCC CAGTGCCGTC TACGATATCT TCATCAGCTA CTGCCAGAAT 
GATCGGACCT GGGTGCTCAA TGAACTGCTG CCGAACGTGG AGGAAACTGG GGATGTGTCC 
ATTTGTCTGC ATGAAAGAGA TTTTCAGATT GGAGTGACCA TCCTAGATAA CATCATCTCC 
TGCATGGATC GCTCGTATTC CCTTATGCTG ATCATATCCT CCAAGTTCCT GTTGAGTCAT 
TGGTGTCAAT TTGAAATGTA TTTGGCACAG CATCGCATCT TTGAGGTCAG CAAGGAGCAC 
TTGATCCTGG TTTTTCTGGA GGACATTCCA CGGCGAAAGC GACCCAAGAC TTTGCAATAT 
CTCATGGATG TGAAAACGTA TATAAAGTGG CCGACGGCCA AGGAGGATCG AAAGCTGTTT 
TGGAAACGTC TGAAGAGATC CCTGGAGGTC ATAGGGATCA ACTCCAGAGA GATCAGTGTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Toll-9 ( NM_001259916.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246845.1 Drosophila melanogaster Toll-9, transcript variant B (Toll-9), mRNA.

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CloneID	OFa21128
Clone ID Related Accession (Same CDS sequence)	NM_001259916.2
Accession Version	NM_001259916.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Toll-9, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001259916.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGTCCAA AATATATATG GGATGTGATC GTATTAGTAT GCCTGTTCCT TGGAAACGTA 
AGGGAAGCAT ACACCGAGTT TTCGATCCAG GATGGCTTGA TAATAGAACC AGATTCAGCC 
ACAACATCCA GCGAAGAGGC CGAAGAGGTC AGCAAGGAGA GAACGGACCT CAAATCCCTC 
ATGTTGAAGT ACGAATCGGA TGATGGGAAC AGTTGTCTGC TGGATCTGAT CAAGGACGAG 
GTGATATGGT GGCAGTTTCC CAACGGAACC CTCAGGGACA GCACAAAGAA ATACGCGCAC 
AAATTGTATT TGGATCTATC CCACGGTAAT CTTAAGGACG ACTCTGACCT ATTCCGAGAA 
GCGAAACTTT CGAGGAAGGT TACCATTTGG AGGACAGAAG TCTTCTCGGC CGCATTCAAT 
ACGTTGACTG CAGCTCCGTT CAGGACGCTC TACTCCATGA GGGAATCCTT GAAGCTTTTA 
TCGCTGCGAG GAAATAACTT TGCTGAACTG ATTCCAGATG CTGAAGCCCA ATCTATAGCC 
TGGGCCACTT TTCCAAAGAT GCCCCGACTT GTGGAGCTGG ACATCAGCAA CTGCAGCATC 
GAGTACGTGA GCAAGGAGGC GTTCCGGAAT GTCAGCAATC TGCGGAGGCT CTTCATGTCG 
GACAACAAGA TCATGACGAT AAGCCACGAC ACTTTCTACT ACGTCCAGGG AGTGCAGTAT 
CTCGACCTGT CCTTCACTAA CTTCCTGACC TATAGCTACC AACTGCAGTT GCCCACTCTG 
GAAATGGCCT TAAGCCTGAT ATACGGCCTG AAGATCCAGC AGAACGTGTT CAAATATCTG 
CCGGAACTGA TTTACCTGGA CCTCTCCCAC TCGAAAATGA CCAGGAATAG TGCGGTGGCT 
TTTGCCCATC TGGGGGATAA GCTGAAGTTC CTCAGTCTGT GCTACACTGC CATTCCCATG 
GTGAGCAGTA CCATCTTCAA GAATACGGTT CTGGAGGGCC TCGACTTGTC CGGAAACCCC 
TACCTATCCT ACAACATCAT CGACGACGCC TTCGATGGCA TCGCTAACAC TCTGAAGTAT 
TTGTACTTCG AGCGATCGAA TATTAAGGAT TTAGAGTGGT CCAAGTCGTT GAAGAACCTT 
CAGGTGTTGG GCCTCGCGGG TAACAACATC AACGCTCTCA CTCCCGCCAT GTTTCAGTCT 
CTGGAGTCCC TGGAGATCCT CGACTTGAGC TCGAACCATG TGGGCAATTG GTACCGCAGT 
GCCTTCCACA ACAACTCCGC CCTAAGGGTT TTGAACCTGA GAAGCAACAC CATCAACATG 
CTGTCCAACG AGATGTTGAA GGACTTTGAG CGATTGGATT ACCTTAGTCT CGGGGACAAC 
GACTTTATTT GCGACTGCCA TTTGAGAGCG GTGGTGGAGG TGGCGGCCGC CAATAATAAA 
GATGCAGACT GCTCCTATAG ACTGCTGAAC TATAGTCAGA ATGCCGTAGG TGAGGAGGTA 
ATATCTCTGG CGGAGTCACT AATTATAGAT CGCAAGCTAT GGCAGAGTCG CTATATTCCC 
TGGCTGCAGA GAAGTTACTC CAACATCAGG GAGTTCAATC GCGCGAATCA TATAATCAAG 
CTGCGCTTCT CCTCCGAGGA CTACATGGTA GCCAAGTGCT CCGCGGCCCA GCCATACCAC 
TTAGGGGACC TTGACGGAGA TCTTACCCTA AAATTTCAGC TGCTCGACTA CGAGGCCAGT 
CAGTATTACT GCTTCAACAA CACCGATCAG CTCCAGGTGG ATGAGCTCAA CTGCCAGATC 
AGGTCGATGA GTGACCTCGC CGAGGAGTTG CACCACGTTA CGAACACAGT GATCGCAGTC 
ATGGGCAGCT TAGTTGGCGC TTGCATCCTG GGATTTATAA TTTATCTGAA GCGCTGGCAC 
ATCCACTACT ATTACTCTTC CCTGAAGTCC GCTGCCTTGT TGTCCAGTGC GTCCAAGGAG 
TCGGTGAATA AATTCACAAA CATATCCCAG AGAGATCCCA GTGCCGTCTA CGATATCTTC 
ATCAGCTACT GCCAGAATGA TCGGACCTGG GTGCTCAATG AACTGCTGCC GAACGTGGAG 
GAAACTGGGG ATGTGTCCAT TTGTCTGCAT GAAAGAGATT TTCAGATTGG AGTGACCATC 
CTAGATAACA TCATCTCCTG CATGGATCGC TCGTATTCCC TTATGCTGAT CATATCCTCC 
AAGTTCCTGT TGAGTCATTG GTGTCAATTT GAAATGTATT TGGCACAGCA TCGCATCTTT 
GAGGTCAGCA AGGAGCACTT GATCCTGGTT TTTCTGGAGG ACATTCCACG GCGAAAGCGA 
CCCAAGACTT TGCAATATCT CATGGATGTG AAAACGTATA TAAAGTGGCC GACGGCCAAG 
GAGGATCGAA AGCTGTTTTG GAAACGTCTG AAGAGATCCC TGGAGGTCAT AGGGATCAAC 
TCCAGAGAGA TCAGTGTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246845.1
CDS	6..2486
Translation	MCPKYIWDVIVLVCLFLGNVREAYTEFSIQDGLIIEPDSATTSSEEAEEVSKERTDLKSLMLKYESDDGNSCLLDLIKDEVIWWQFPNGTLRDSTKKYAHKLYLDLSHGNLKDDSDLFREAKLSRKVTIWRTEVFSAAFNTLTAAPFRTLYSMRESLKLLSLRGNNFAELIPDAEAQSIAWATFPKMPRLVELDISNCSIEYVSKEAFRNVSNLRRLFMSDNKIMTISHDTFYYVQGVQYLDLSFTNFLTYSYQLQLPTLEMALSLIYGLKIQQNVFKYLPELIYLDLSHSKMTRNSAVAFAHLGDKLKFLSLCYTAIPMVSSTIFKNTVLEGLDLSGNPYLSYNIIDDAFDGIANTLKYLYFERSNIKDLEWSKSLKNLQVLGLAGNNINALTPAMFQSLESLEILDLSSNHVGNWYRSAFHNNSALRVLNLRSNTINMLSNEMLKDFERLDYLSLGDNDFICDCHLRAVVEVAAANNKDADCSYRLLNYSQNAVGEEVISLAESLIIDRKLWQSRYIPWLQRSYSNIREFNRANHIIKLRFSSEDYMVAKCSAAQPYHLGDLDGDLTLKFQLLDYEASQYYCFNNTDQLQVDELNCQIRSMSDLAEELHHVTNTVIAVMGSLVGACILGFIIYLKRWHIHYYYSSLKSAALLSSASKESVNKFTNISQRDPSAVYDIFISYCQNDRTWVLNELLPNVEETGDVSICLHERDFQIGVTILDNIISCMDRSYSLMLIISSKFLLSHWCQFEMYLAQHRIFEVSKEHLILVFLEDIPRRKRPKTLQYLMDVKTYIKWPTAKEDRKLFWKRLKRSLEVIGINSREISV

Target ORF information:

RefSeq Version	NM_001259916.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Toll-9, transcript variant B (Toll-9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259916.2

ORF Insert Sequence:

ATGTGTCCAA AATATATATG GGATGTGATC GTATTAGTAT GCCTGTTCCT TGGAAACGTA 
AGGGAAGCAT ACACCGAGTT TTCGATCCAG GATGGCTTGA TAATAGAACC AGATTCAGCC 
ACAACATCCA GCGAAGAGGC CGAAGAGGTC AGCAAGGAGA GAACGGACCT CAAATCCCTC 
ATGTTGAAGT ACGAATCGGA TGATGGGAAC AGTTGTCTGC TGGATCTGAT CAAGGACGAG 
GTGATATGGT GGCAGTTTCC CAACGGAACC CTCAGGGACA GCACAAAGAA ATACGCGCAC 
AAATTGTATT TGGATCTATC CCACGGTAAT CTTAAGGACG ACTCTGACCT ATTCCGAGAA 
GCGAAACTTT CGAGGAAGGT TACCATTTGG AGGACAGAAG TCTTCTCGGC CGCATTCAAT 
ACGTTGACTG CAGCTCCGTT CAGGACGCTC TACTCCATGA GGGAATCCTT GAAGCTTTTA 
TCGCTGCGAG GAAATAACTT TGCTGAACTG ATTCCAGATG CTGAAGCCCA ATCTATAGCC 
TGGGCCACTT TTCCAAAGAT GCCCCGACTT GTGGAGCTGG ACATCAGCAA CTGCAGCATC 
GAGTACGTGA GCAAGGAGGC GTTCCGGAAT GTCAGCAATC TGCGGAGGCT CTTCATGTCG 
GACAACAAGA TCATGACGAT AAGCCACGAC ACTTTCTACT ACGTCCAGGG AGTGCAGTAT 
CTCGACCTGT CCTTCACTAA CTTCCTGACC TATAGCTACC AACTGCAGTT GCCCACTCTG 
GAAATGGCCT TAAGCCTGAT ATACGGCCTG AAGATCCAGC AGAACGTGTT CAAATATCTG 
CCGGAACTGA TTTACCTGGA CCTCTCCCAC TCGAAAATGA CCAGGAATAG TGCGGTGGCT 
TTTGCCCATC TGGGGGATAA GCTGAAGTTC CTCAGTCTGT GCTACACTGC CATTCCCATG 
GTGAGCAGTA CCATCTTCAA GAATACGGTT CTGGAGGGCC TCGACTTGTC CGGAAACCCC 
TACCTATCCT ACAACATCAT CGACGACGCC TTCGATGGCA TCGCTAACAC TCTGAAGTAT 
TTGTACTTCG AGCGATCGAA TATTAAGGAT TTAGAGTGGT CCAAGTCGTT GAAGAACCTT 
CAGGTGTTGG GCCTCGCGGG TAACAACATC AACGCTCTCA CTCCCGCCAT GTTTCAGTCT 
CTGGAGTCCC TGGAGATCCT CGACTTGAGC TCGAACCATG TGGGCAATTG GTACCGCAGT 
GCCTTCCACA ACAACTCCGC CCTAAGGGTT TTGAACCTGA GAAGCAACAC CATCAACATG 
CTGTCCAACG AGATGTTGAA GGACTTTGAG CGATTGGATT ACCTTAGTCT CGGGGACAAC 
GACTTTATTT GCGACTGCCA TTTGAGAGCG GTGGTGGAGG TGGCGGCCGC CAATAATAAA 
GATGCAGACT GCTCCTATAG ACTGCTGAAC TATAGTCAGA ATGCCGTAGG TGAGGAGGTA 
ATATCTCTGG CGGAGTCACT AATTATAGAT CGCAAGCTAT GGCAGAGTCG CTATATTCCC 
TGGCTGCAGA GAAGTTACTC CAACATCAGG GAGTTCAATC GCGCGAATCA TATAATCAAG 
CTGCGCTTCT CCTCCGAGGA CTACATGGTA GCCAAGTGCT CCGCGGCCCA GCCATACCAC 
TTAGGGGACC TTGACGGAGA TCTTACCCTA AAATTTCAGC TGCTCGACTA CGAGGCCAGT 
CAGTATTACT GCTTCAACAA CACCGATCAG CTCCAGGTGG ATGAGCTCAA CTGCCAGATC 
AGGTCGATGA GTGACCTCGC CGAGGAGTTG CACCACGTTA CGAACACAGT GATCGCAGTC 
ATGGGCAGCT TAGTTGGCGC TTGCATCCTG GGATTTATAA TTTATCTGAA GCGCTGGCAC 
ATCCACTACT ATTACTCTTC CCTGAAGTCC GCTGCCTTGT TGTCCAGTGC GTCCAAGGAG 
TCGGTGAATA AATTCACAAA CATATCCCAG AGAGATCCCA GTGCCGTCTA CGATATCTTC 
ATCAGCTACT GCCAGAATGA TCGGACCTGG GTGCTCAATG AACTGCTGCC GAACGTGGAG 
GAAACTGGGG ATGTGTCCAT TTGTCTGCAT GAAAGAGATT TTCAGATTGG AGTGACCATC 
CTAGATAACA TCATCTCCTG CATGGATCGC TCGTATTCCC TTATGCTGAT CATATCCTCC 
AAGTTCCTGT TGAGTCATTG GTGTCAATTT GAAATGTATT TGGCACAGCA TCGCATCTTT 
GAGGTCAGCA AGGAGCACTT GATCCTGGTT TTTCTGGAGG ACATTCCACG GCGAAAGCGA 
CCCAAGACTT TGCAATATCT CATGGATGTG AAAACGTATA TAAAGTGGCC GACGGCCAAG 
GAGGATCGAA AGCTGTTTTG GAAACGTCTG AAGAGATCCC TGGAGGTCAT AGGGATCAAC 
TCCAGAGAGA TCAGTGTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Toll-9 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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