Su(var)3-3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Su(var)3-3
Entrez Gene ID	40217
Full Name	Suppressor of variegation 3-3
Synonyms	CG17149,Dmel/HDM,Dmel/LSD1,Dmel\CG17149,Dmel\LSD1,Hdm,LSD1,Lsd1,SU(VAR)3-3,Su-var(3)3,Suvar(3)3,dLSD1,dLsd1,lsd1
General protein information	Preferred Names Suppressor of variegation 3-3 Names CG17149 gene product from transcript CG17149-RA CG17149-PA CG17149-PB CG17149-PC Su(var)3-3-PA Su(var)3-3-PB Su(var)3-3-PC flavoprotein histone demethylase histone lysine-specific demethylase 1 lysine-specific demethylase 1 suppressor of variegation 3-3
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 77A3-77A3\|3-46.6 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_140937.3	NP_649194.1	suppressor of variegation 3-3, isoform A
NM_168835.2	NP_730497.1	suppressor of variegation 3-3, isoform B
NM_001275171.1	NP_001262100.1	suppressor of variegation 3-3, isoform C

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	KDM1A	XP_005617878.1
Mus musculus (house mouse)	Kdm1a	NP_598633.2
Gallus gallus (chicken)	KDM1A	XP_417719.4
Danio rerio (zebrafish)	kdm1a	NP_001229924.1
Caenorhabditis elegans (roundworm)	spr-5	NP_493366.1
Caenorhabditis elegans (roundworm)	lsd-1	NP_510000.2
Neurospora crassa	NCU09120	XP_958892.1
Oryza sativa (rice)	Os08g0143400	NP_001060976.1
Rattus norvegicus (Norway rat)	Kdm1a	NP_001123570.1
Drosophila melanogaster (fruit fly)	Su(var)3-3	NP_649194.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011661	XP_320852.4
Arabidopsis thaliana (thale cress)	LDL2	NP_187981.1
Pan troglodytes (chimpanzee)	KDM1A	XP_513190.3
Bos taurus (cattle)	KDM1A	XP_002685762.1
Magnaporthe oryzae (rice blast fungus)	MGG_09915	XP_003710013.1
Xenopus tropicalis (tropical clawed frog)	kdm1a	XP_002936640.1
Homo sapiens (human)	KDM1A	NP_001009999.1
	KDM1A	XP_001111525.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

identified lysine-specific demethylase 1 (lsd1) and CoRest as differentiation regulators
Title: The progenitor state is maintained by lysine-specific demethylase 1-mediated epigenetic plasticity during Drosophila follicle cell development.

Lsd1 functions to repress decapentaplegic (dpp) expression in adult germaria
Title: Loss of lysine-specific demethylase 1 nonautonomously causes stem cell tumors in the Drosophila ovary.

found that the Lysine Specific Demethylase 1 gene (lsd1) was important for the proper cell type-specific development of wing patterning
Title: Histone lysine demethylases function as co-repressors of SWI/SNF remodeling activities during Drosophila wing development.

Mutation of Drosophila Lsd1 disrupts H3-K4 methylation, resulting in tissue-specific defects during development.
Title: Mutation of Drosophila Lsd1 disrupts H3-K4 methylation, resulting in tissue-specific defects during development.

The following Su(var)3-3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Su(var)3-3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19074	NM_140937.3 Latest version!	Drosophila melanogaster suppressor of variegation 3-3, transcript variant A (Su(var)3-3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa19074	NM_168835.2 Latest version!	Drosophila melanogaster suppressor of variegation 3-3, transcript variant B (Su(var)3-3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa21119	NM_001275171.1 Latest version!	Drosophila melanogaster suppressor of variegation 3-3, transcript variant C (Su(var)3-3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Su(var)3-3 ( NM_140937.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649194.1 Drosophila melanogaster suppressor of variegation 3-3, transcript variant A (Su(var)3-3), mRNA.

Price & Availability↑

CloneID	OFa19074
Clone ID Related Accession (Same CDS sequence)	NM_140937.3 , NM_168835.2
Accession Version	NM_140937.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	suppressor of variegation 3-3, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140937.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAACCCA CCCAGTTCGG AGGCAGTAGC TCCAAGATGA CCGAGCCAAT TGAGTATGTG 
ACGCTAATTA GCGACGATTC CGATGGTGAG CCCACGCCCA AAAGAAACGT CAATCACCCG 
CCGTCGGCAT TGTCAGCACC CAATCCTGGT CAGAAACAAA AGCACCCGGA TGAGGACTCA 
AATGACGCAC CTGCCACCAG CGATGAGCGA CGCACAAGTA GGCGCAATCG GCCAAAAGTG 
GATTATAGTA ACAGGCCTAG TGGAAGTGGA GACACCGCCT CCAATGACAA GTCAGGATCG 
GCATCCATGG GACCTAACAA CCAGCAAGCA GAGCGTCGCA GCCAAAGCCA AACCCGCAAG 
TCAGAAGCCA ATGCTACCTC CTCCTCCGTG TCCGGACCCA GTGCCGGAAA TTCCAGACCG 
TCCCAGAACG GAGATTCCAA GGACCGGGAT GCCGGCACGC CCACGGTTTT GTCTGGCCAA 
GAGGGCGCCG TCTTCCAATC TCGCCTGCCA TTTAACAAAA TGACACCCAA CGAAGAGGCC 
TGCTTTCCGG ACATCAGTCG CTCGGGCATT CTCGGCCATC GGGTATTCCT CAACATTCGT 
AACAGCCTCT TGCACATGTG GGTGGACAAT CCCAAGATCC AGCTGAGCTT CGAGATCGCC 
CTGAAGAACC TGCCGCCGCC ATTCGACAGC GAGCCCAGTT TGGTAAGGCG AGTACACTCG 
TTTCTGGAGC GTCATGGCTT CATTAACTTC GGCATCTTTA AGCGACTTAA GCCTATACCC 
GCCAAAAAGC TGGGCAAAGT CATAGTGATT GGGGCAGGGA TCTCCGGCTT AGCAGTGGCA 
CACCAGTTGC AGCAGTTCGG CATGGATGTG ATCGTCTTGG AAGCGCGGGA TCGGGTAGGG 
GGACGGATAT CTACGTTTCG GAAGAACAGC TATATTGCCG ATGTGGGAGC AATGGTGGTT 
ACCGGCGTCT ATGGCAATCC AATGACGATC CTCAGCAAGC AGATCGGTAT GGACCTAGTT 
CCCATCCAGC AGACATGTCC ACTTTACGGC CCAGATGGAA AGCCCGTGCC CAAGGAGAAG 
GATGACGTGA TCGAGAGAGA ATTTAACCGA CTGCTGGAAT CAGCCAGCTA CCTGTCCCAC 
CGCCTTGATT TTAACTACGC CGGCGATTGC CCAGTTTCGC TGGGCGATGC CTTGGAGTGG 
ATAATCAGTA TGCAGGAGAT GCAGGTGATG CACAAACGGG GGCAGCACAT GCAGGAGATC 
ATAGCCACCC AAACGAAGAT CATTGAACAG CGTCGTCGAC TGAAAACCCT TAGAGATACC 
ATCGGCAAAC TAAAGAATGA GCACTTGGCA ATGATCAACC AGCGGAAACC CAAAGGCACG 
GATGGCGATC TGAAATACTG TTACCAAGAG TTTAACATTC GGAACACCCA GATCAAAATG 
GAGGAAACGA TCAGTACGTT TCACGATCTC CATGCCGAGG AAAAGCAGAT GTTAGCCAAA 
CTCCACGAAC TGGAGCAGAA CCGACCTAGC GATGTGTACC TCTCGTCCCG CGACCGCCTG 
ATCTTGGACT GGCATTTTGC CAACTTGGAA TTTGCTAATG CCACCCGACT AAATAACCTG 
TCGTTGAAGC ACTGGGATCA GGACGATGAC TTCGAGTTTA TTGGCCACCA CACAACTGTG 
CGGAACGGTT ATTCATGTGT GCCTGTAGCT CTTACTGAAA ACCTGGACAT CCGTGTCAAT 
AGCGCCGTCA AGGAGATAAA GTACGGCACC AAAGGTGTGG AAGTAGTGGC TGAAAACTTG 
AAGACATCCA ATTCGCAGAT GACATACAAG GCAGACCTTG TCGTCTGCAC ACTTACTCTG 
GGCGTCCTCA AAGTGGCGGT AGCACATAAG GAGTCACAGC AGAGTAACAC AGTCAAGTTC 
GACCCGCCAT TGCCGGACTG GAAACAGCAG GCCATCAAAA GGCTGGGATT TGGGAATCTC 
AATAAGGTTG TGCTCTGCTT CGATCGCATT TTTTGGGATC CCAACGCTAA TCTCTTCGGA 
CACGTGGGCA GCACGACGGC GAGTAGAGGA GAAATGTTTC TGTTCTGGAG TATAAGCTCA 
TCTCCGGTGT TGCTCGCTCT AGTCGCCGGA ATGGCAGCGA ATCTGGTGGA GAGCGTGACG 
GATGACATCA TAATCGGGCG TTGTATGTCG GTGCTAAAAA ACATCTTCGG GAATACCTCT 
GTTCCACAAC CCAAGGAAAC AGTGGTAACG CGTTGGCGCA GCGATCCCTG GGCCCGTGGG 
TCGTACAGCT ACGTATCCGT TGGTTCCTCG GGCAGTGACT ACGATCTGCT GGCGGCGCCG 
GTCATTCCGC CGTCCAGTAA GGATGCGGAA GGATTGCCAC GGCTATTCTT CGCGGGTGAG 
CATACGATAC GGAATTATCC GGCCACTGTG CATGGAGCGT ACTTGAGCGG ATTGCGCGAG 
GCGGGACGTA TTGCGGATTA CTATCTCGGC TATCCAGAGG GAACACCGCC GGACATTGGC 
TACTCGGTGG CTGAGGCGGC CAATCTGGTG TCCGTGGGAA ATGTTGTCAA GCTGCGCGAC 
CTCTCGCCCA ATCTATCTGA CTCCTCCCCG TCGTCAAAGA AGTCGGAGGA GAATTCAAAC 
TCAAACACTG CCGACTCTAC GGAGCTACAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649194.1
CDS	436..3108
Translation	MKPTQFGGSSSKMTEPIEYVTLISDDSDGEPTPKRNVNHPPSALSAPNPGQKQKHPDEDSNDAPATSDERRTSRRNRPKVDYSNRPSGSGDTASNDKSGSASMGPNNQQAERRSQSQTRKSEANATSSSVSGPSAGNSRPSQNGDSKDRDAGTPTVLSGQEGAVFQSRLPFNKMTPNEEACFPDISRSGILGHRVFLNIRNSLLHMWVDNPKIQLSFEIALKNLPPPFDSEPSLVRRVHSFLERHGFINFGIFKRLKPIPAKKLGKVIVIGAGISGLAVAHQLQQFGMDVIVLEARDRVGGRISTFRKNSYIADVGAMVVTGVYGNPMTILSKQIGMDLVPIQQTCPLYGPDGKPVPKEKDDVIEREFNRLLESASYLSHRLDFNYAGDCPVSLGDALEWIISMQEMQVMHKRGQHMQEIIATQTKIIEQRRRLKTLRDTIGKLKNEHLAMINQRKPKGTDGDLKYCYQEFNIRNTQIKMEETISTFHDLHAEEKQMLAKLHELEQNRPSDVYLSSRDRLILDWHFANLEFANATRLNNLSLKHWDQDDDFEFIGHHTTVRNGYSCVPVALTENLDIRVNSAVKEIKYGTKGVEVVAENLKTSNSQMTYKADLVVCTLTLGVLKVAVAHKESQQSNTVKFDPPLPDWKQQAIKRLGFGNLNKVVLCFDRIFWDPNANLFGHVGSTTASRGEMFLFWSISSSPVLLALVAGMAANLVESVTDDIIIGRCMSVLKNIFGNTSVPQPKETVVTRWRSDPWARGSYSYVSVGSSGSDYDLLAAPVIPPSSKDAEGLPRLFFAGEHTIRNYPATVHGAYLSGLREAGRIADYYLGYPEGTPPDIGYSVAEAANLVSVGNVVKLRDLSPNLSDSSPSSKKSEENSNSNTADSTELQ

Target ORF information:

RefSeq Version	NM_140937.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster suppressor of variegation 3-3, transcript variant A (Su(var)3-3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140937.3

ORF Insert Sequence:

ATGAAACCCA CCCAGTTCGG AGGCAGTAGC TCCAAGATGA CCGAGCCAAT TGAGTATGTG 
ACGCTAATTA GCGACGATTC CGATGGTGAG CCCACGCCCA AAAGAAACGT CAATCACCCG 
CCGTCGGCAT TGTCAGCACC CAATCCTGGT CAGAAACAAA AGCACCCGGA TGAGGACTCA 
AATGACGCAC CTGCCACCAG CGATGAGCGA CGCACAAGTA GGCGCAATCG GCCAAAAGTG 
GATTATAGTA ACAGGCCTAG TGGAAGTGGA GACACCGCCT CCAATGACAA GTCAGGATCG 
GCATCCATGG GACCTAACAA CCAGCAAGCA GAGCGTCGCA GCCAAAGCCA AACCCGCAAG 
TCAGAAGCCA ATGCTACCTC CTCCTCCGTG TCCGGACCCA GTGCCGGAAA TTCCAGACCG 
TCCCAGAACG GAGATTCCAA GGACCGGGAT GCCGGCACGC CCACGGTTTT GTCTGGCCAA 
GAGGGCGCCG TCTTCCAATC TCGCCTGCCA TTTAACAAAA TGACACCCAA CGAAGAGGCC 
TGCTTTCCGG ACATCAGTCG CTCGGGCATT CTCGGCCATC GGGTATTCCT CAACATTCGT 
AACAGCCTCT TGCACATGTG GGTGGACAAT CCCAAGATCC AGCTGAGCTT CGAGATCGCC 
CTGAAGAACC TGCCGCCGCC ATTCGACAGC GAGCCCAGTT TGGTAAGGCG AGTACACTCG 
TTTCTGGAGC GTCATGGCTT CATTAACTTC GGCATCTTTA AGCGACTTAA GCCTATACCC 
GCCAAAAAGC TGGGCAAAGT CATAGTGATT GGGGCAGGGA TCTCCGGCTT AGCAGTGGCA 
CACCAGTTGC AGCAGTTCGG CATGGATGTG ATCGTCTTGG AAGCGCGGGA TCGGGTAGGG 
GGACGGATAT CTACGTTTCG GAAGAACAGC TATATTGCCG ATGTGGGAGC AATGGTGGTT 
ACCGGCGTCT ATGGCAATCC AATGACGATC CTCAGCAAGC AGATCGGTAT GGACCTAGTT 
CCCATCCAGC AGACATGTCC ACTTTACGGC CCAGATGGAA AGCCCGTGCC CAAGGAGAAG 
GATGACGTGA TCGAGAGAGA ATTTAACCGA CTGCTGGAAT CAGCCAGCTA CCTGTCCCAC 
CGCCTTGATT TTAACTACGC CGGCGATTGC CCAGTTTCGC TGGGCGATGC CTTGGAGTGG 
ATAATCAGTA TGCAGGAGAT GCAGGTGATG CACAAACGGG GGCAGCACAT GCAGGAGATC 
ATAGCCACCC AAACGAAGAT CATTGAACAG CGTCGTCGAC TGAAAACCCT TAGAGATACC 
ATCGGCAAAC TAAAGAATGA GCACTTGGCA ATGATCAACC AGCGGAAACC CAAAGGCACG 
GATGGCGATC TGAAATACTG TTACCAAGAG TTTAACATTC GGAACACCCA GATCAAAATG 
GAGGAAACGA TCAGTACGTT TCACGATCTC CATGCCGAGG AAAAGCAGAT GTTAGCCAAA 
CTCCACGAAC TGGAGCAGAA CCGACCTAGC GATGTGTACC TCTCGTCCCG CGACCGCCTG 
ATCTTGGACT GGCATTTTGC CAACTTGGAA TTTGCTAATG CCACCCGACT AAATAACCTG 
TCGTTGAAGC ACTGGGATCA GGACGATGAC TTCGAGTTTA TTGGCCACCA CACAACTGTG 
CGGAACGGTT ATTCATGTGT GCCTGTAGCT CTTACTGAAA ACCTGGACAT CCGTGTCAAT 
AGCGCCGTCA AGGAGATAAA GTACGGCACC AAAGGTGTGG AAGTAGTGGC TGAAAACTTG 
AAGACATCCA ATTCGCAGAT GACATACAAG GCAGACCTTG TCGTCTGCAC ACTTACTCTG 
GGCGTCCTCA AAGTGGCGGT AGCACATAAG GAGTCACAGC AGAGTAACAC AGTCAAGTTC 
GACCCGCCAT TGCCGGACTG GAAACAGCAG GCCATCAAAA GGCTGGGATT TGGGAATCTC 
AATAAGGTTG TGCTCTGCTT CGATCGCATT TTTTGGGATC CCAACGCTAA TCTCTTCGGA 
CACGTGGGCA GCACGACGGC GAGTAGAGGA GAAATGTTTC TGTTCTGGAG TATAAGCTCA 
TCTCCGGTGT TGCTCGCTCT AGTCGCCGGA ATGGCAGCGA ATCTGGTGGA GAGCGTGACG 
GATGACATCA TAATCGGGCG TTGTATGTCG GTGCTAAAAA ACATCTTCGG GAATACCTCT 
GTTCCACAAC CCAAGGAAAC AGTGGTAACG CGTTGGCGCA GCGATCCCTG GGCCCGTGGG 
TCGTACAGCT ACGTATCCGT TGGTTCCTCG GGCAGTGACT ACGATCTGCT GGCGGCGCCG 
GTCATTCCGC CGTCCAGTAA GGATGCGGAA GGATTGCCAC GGCTATTCTT CGCGGGTGAG 
CATACGATAC GGAATTATCC GGCCACTGTG CATGGAGCGT ACTTGAGCGG ATTGCGCGAG 
GCGGGACGTA TTGCGGATTA CTATCTCGGC TATCCAGAGG GAACACCGCC GGACATTGGC 
TACTCGGTGG CTGAGGCGGC CAATCTGGTG TCCGTGGGAA ATGTTGTCAA GCTGCGCGAC 
CTCTCGCCCA ATCTATCTGA CTCCTCCCCG TCGTCAAAGA AGTCGGAGGA GAATTCAAAC 
TCAAACACTG CCGACTCTAC GGAGCTACAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Su(var)3-3 ( NM_168835.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730497.1 Drosophila melanogaster suppressor of variegation 3-3, transcript variant B (Su(var)3-3), mRNA.

Price & Availability↑

CloneID	OFa19074
Clone ID Related Accession (Same CDS sequence)	NM_140937.3 , NM_168835.2
Accession Version	NM_168835.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	suppressor of variegation 3-3, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168835.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAACCCA CCCAGTTCGG AGGCAGTAGC TCCAAGATGA CCGAGCCAAT TGAGTATGTG 
ACGCTAATTA GCGACGATTC CGATGGTGAG CCCACGCCCA AAAGAAACGT CAATCACCCG 
CCGTCGGCAT TGTCAGCACC CAATCCTGGT CAGAAACAAA AGCACCCGGA TGAGGACTCA 
AATGACGCAC CTGCCACCAG CGATGAGCGA CGCACAAGTA GGCGCAATCG GCCAAAAGTG 
GATTATAGTA ACAGGCCTAG TGGAAGTGGA GACACCGCCT CCAATGACAA GTCAGGATCG 
GCATCCATGG GACCTAACAA CCAGCAAGCA GAGCGTCGCA GCCAAAGCCA AACCCGCAAG 
TCAGAAGCCA ATGCTACCTC CTCCTCCGTG TCCGGACCCA GTGCCGGAAA TTCCAGACCG 
TCCCAGAACG GAGATTCCAA GGACCGGGAT GCCGGCACGC CCACGGTTTT GTCTGGCCAA 
GAGGGCGCCG TCTTCCAATC TCGCCTGCCA TTTAACAAAA TGACACCCAA CGAAGAGGCC 
TGCTTTCCGG ACATCAGTCG CTCGGGCATT CTCGGCCATC GGGTATTCCT CAACATTCGT 
AACAGCCTCT TGCACATGTG GGTGGACAAT CCCAAGATCC AGCTGAGCTT CGAGATCGCC 
CTGAAGAACC TGCCGCCGCC ATTCGACAGC GAGCCCAGTT TGGTAAGGCG AGTACACTCG 
TTTCTGGAGC GTCATGGCTT CATTAACTTC GGCATCTTTA AGCGACTTAA GCCTATACCC 
GCCAAAAAGC TGGGCAAAGT CATAGTGATT GGGGCAGGGA TCTCCGGCTT AGCAGTGGCA 
CACCAGTTGC AGCAGTTCGG CATGGATGTG ATCGTCTTGG AAGCGCGGGA TCGGGTAGGG 
GGACGGATAT CTACGTTTCG GAAGAACAGC TATATTGCCG ATGTGGGAGC AATGGTGGTT 
ACCGGCGTCT ATGGCAATCC AATGACGATC CTCAGCAAGC AGATCGGTAT GGACCTAGTT 
CCCATCCAGC AGACATGTCC ACTTTACGGC CCAGATGGAA AGCCCGTGCC CAAGGAGAAG 
GATGACGTGA TCGAGAGAGA ATTTAACCGA CTGCTGGAAT CAGCCAGCTA CCTGTCCCAC 
CGCCTTGATT TTAACTACGC CGGCGATTGC CCAGTTTCGC TGGGCGATGC CTTGGAGTGG 
ATAATCAGTA TGCAGGAGAT GCAGGTGATG CACAAACGGG GGCAGCACAT GCAGGAGATC 
ATAGCCACCC AAACGAAGAT CATTGAACAG CGTCGTCGAC TGAAAACCCT TAGAGATACC 
ATCGGCAAAC TAAAGAATGA GCACTTGGCA ATGATCAACC AGCGGAAACC CAAAGGCACG 
GATGGCGATC TGAAATACTG TTACCAAGAG TTTAACATTC GGAACACCCA GATCAAAATG 
GAGGAAACGA TCAGTACGTT TCACGATCTC CATGCCGAGG AAAAGCAGAT GTTAGCCAAA 
CTCCACGAAC TGGAGCAGAA CCGACCTAGC GATGTGTACC TCTCGTCCCG CGACCGCCTG 
ATCTTGGACT GGCATTTTGC CAACTTGGAA TTTGCTAATG CCACCCGACT AAATAACCTG 
TCGTTGAAGC ACTGGGATCA GGACGATGAC TTCGAGTTTA TTGGCCACCA CACAACTGTG 
CGGAACGGTT ATTCATGTGT GCCTGTAGCT CTTACTGAAA ACCTGGACAT CCGTGTCAAT 
AGCGCCGTCA AGGAGATAAA GTACGGCACC AAAGGTGTGG AAGTAGTGGC TGAAAACTTG 
AAGACATCCA ATTCGCAGAT GACATACAAG GCAGACCTTG TCGTCTGCAC ACTTACTCTG 
GGCGTCCTCA AAGTGGCGGT AGCACATAAG GAGTCACAGC AGAGTAACAC AGTCAAGTTC 
GACCCGCCAT TGCCGGACTG GAAACAGCAG GCCATCAAAA GGCTGGGATT TGGGAATCTC 
AATAAGGTTG TGCTCTGCTT CGATCGCATT TTTTGGGATC CCAACGCTAA TCTCTTCGGA 
CACGTGGGCA GCACGACGGC GAGTAGAGGA GAAATGTTTC TGTTCTGGAG TATAAGCTCA 
TCTCCGGTGT TGCTCGCTCT AGTCGCCGGA ATGGCAGCGA ATCTGGTGGA GAGCGTGACG 
GATGACATCA TAATCGGGCG TTGTATGTCG GTGCTAAAAA ACATCTTCGG GAATACCTCT 
GTTCCACAAC CCAAGGAAAC AGTGGTAACG CGTTGGCGCA GCGATCCCTG GGCCCGTGGG 
TCGTACAGCT ACGTATCCGT TGGTTCCTCG GGCAGTGACT ACGATCTGCT GGCGGCGCCG 
GTCATTCCGC CGTCCAGTAA GGATGCGGAA GGATTGCCAC GGCTATTCTT CGCGGGTGAG 
CATACGATAC GGAATTATCC GGCCACTGTG CATGGAGCGT ACTTGAGCGG ATTGCGCGAG 
GCGGGACGTA TTGCGGATTA CTATCTCGGC TATCCAGAGG GAACACCGCC GGACATTGGC 
TACTCGGTGG CTGAGGCGGC CAATCTGGTG TCCGTGGGAA ATGTTGTCAA GCTGCGCGAC 
CTCTCGCCCA ATCTATCTGA CTCCTCCCCG TCGTCAAAGA AGTCGGAGGA GAATTCAAAC 
TCAAACACTG CCGACTCTAC GGAGCTACAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730497.1
CDS	370..3042
Translation	MKPTQFGGSSSKMTEPIEYVTLISDDSDGEPTPKRNVNHPPSALSAPNPGQKQKHPDEDSNDAPATSDERRTSRRNRPKVDYSNRPSGSGDTASNDKSGSASMGPNNQQAERRSQSQTRKSEANATSSSVSGPSAGNSRPSQNGDSKDRDAGTPTVLSGQEGAVFQSRLPFNKMTPNEEACFPDISRSGILGHRVFLNIRNSLLHMWVDNPKIQLSFEIALKNLPPPFDSEPSLVRRVHSFLERHGFINFGIFKRLKPIPAKKLGKVIVIGAGISGLAVAHQLQQFGMDVIVLEARDRVGGRISTFRKNSYIADVGAMVVTGVYGNPMTILSKQIGMDLVPIQQTCPLYGPDGKPVPKEKDDVIEREFNRLLESASYLSHRLDFNYAGDCPVSLGDALEWIISMQEMQVMHKRGQHMQEIIATQTKIIEQRRRLKTLRDTIGKLKNEHLAMINQRKPKGTDGDLKYCYQEFNIRNTQIKMEETISTFHDLHAEEKQMLAKLHELEQNRPSDVYLSSRDRLILDWHFANLEFANATRLNNLSLKHWDQDDDFEFIGHHTTVRNGYSCVPVALTENLDIRVNSAVKEIKYGTKGVEVVAENLKTSNSQMTYKADLVVCTLTLGVLKVAVAHKESQQSNTVKFDPPLPDWKQQAIKRLGFGNLNKVVLCFDRIFWDPNANLFGHVGSTTASRGEMFLFWSISSSPVLLALVAGMAANLVESVTDDIIIGRCMSVLKNIFGNTSVPQPKETVVTRWRSDPWARGSYSYVSVGSSGSDYDLLAAPVIPPSSKDAEGLPRLFFAGEHTIRNYPATVHGAYLSGLREAGRIADYYLGYPEGTPPDIGYSVAEAANLVSVGNVVKLRDLSPNLSDSSPSSKKSEENSNSNTADSTELQ

Target ORF information:

RefSeq Version	NM_168835.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster suppressor of variegation 3-3, transcript variant B (Su(var)3-3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168835.2

ORF Insert Sequence:

ATGAAACCCA CCCAGTTCGG AGGCAGTAGC TCCAAGATGA CCGAGCCAAT TGAGTATGTG 
ACGCTAATTA GCGACGATTC CGATGGTGAG CCCACGCCCA AAAGAAACGT CAATCACCCG 
CCGTCGGCAT TGTCAGCACC CAATCCTGGT CAGAAACAAA AGCACCCGGA TGAGGACTCA 
AATGACGCAC CTGCCACCAG CGATGAGCGA CGCACAAGTA GGCGCAATCG GCCAAAAGTG 
GATTATAGTA ACAGGCCTAG TGGAAGTGGA GACACCGCCT CCAATGACAA GTCAGGATCG 
GCATCCATGG GACCTAACAA CCAGCAAGCA GAGCGTCGCA GCCAAAGCCA AACCCGCAAG 
TCAGAAGCCA ATGCTACCTC CTCCTCCGTG TCCGGACCCA GTGCCGGAAA TTCCAGACCG 
TCCCAGAACG GAGATTCCAA GGACCGGGAT GCCGGCACGC CCACGGTTTT GTCTGGCCAA 
GAGGGCGCCG TCTTCCAATC TCGCCTGCCA TTTAACAAAA TGACACCCAA CGAAGAGGCC 
TGCTTTCCGG ACATCAGTCG CTCGGGCATT CTCGGCCATC GGGTATTCCT CAACATTCGT 
AACAGCCTCT TGCACATGTG GGTGGACAAT CCCAAGATCC AGCTGAGCTT CGAGATCGCC 
CTGAAGAACC TGCCGCCGCC ATTCGACAGC GAGCCCAGTT TGGTAAGGCG AGTACACTCG 
TTTCTGGAGC GTCATGGCTT CATTAACTTC GGCATCTTTA AGCGACTTAA GCCTATACCC 
GCCAAAAAGC TGGGCAAAGT CATAGTGATT GGGGCAGGGA TCTCCGGCTT AGCAGTGGCA 
CACCAGTTGC AGCAGTTCGG CATGGATGTG ATCGTCTTGG AAGCGCGGGA TCGGGTAGGG 
GGACGGATAT CTACGTTTCG GAAGAACAGC TATATTGCCG ATGTGGGAGC AATGGTGGTT 
ACCGGCGTCT ATGGCAATCC AATGACGATC CTCAGCAAGC AGATCGGTAT GGACCTAGTT 
CCCATCCAGC AGACATGTCC ACTTTACGGC CCAGATGGAA AGCCCGTGCC CAAGGAGAAG 
GATGACGTGA TCGAGAGAGA ATTTAACCGA CTGCTGGAAT CAGCCAGCTA CCTGTCCCAC 
CGCCTTGATT TTAACTACGC CGGCGATTGC CCAGTTTCGC TGGGCGATGC CTTGGAGTGG 
ATAATCAGTA TGCAGGAGAT GCAGGTGATG CACAAACGGG GGCAGCACAT GCAGGAGATC 
ATAGCCACCC AAACGAAGAT CATTGAACAG CGTCGTCGAC TGAAAACCCT TAGAGATACC 
ATCGGCAAAC TAAAGAATGA GCACTTGGCA ATGATCAACC AGCGGAAACC CAAAGGCACG 
GATGGCGATC TGAAATACTG TTACCAAGAG TTTAACATTC GGAACACCCA GATCAAAATG 
GAGGAAACGA TCAGTACGTT TCACGATCTC CATGCCGAGG AAAAGCAGAT GTTAGCCAAA 
CTCCACGAAC TGGAGCAGAA CCGACCTAGC GATGTGTACC TCTCGTCCCG CGACCGCCTG 
ATCTTGGACT GGCATTTTGC CAACTTGGAA TTTGCTAATG CCACCCGACT AAATAACCTG 
TCGTTGAAGC ACTGGGATCA GGACGATGAC TTCGAGTTTA TTGGCCACCA CACAACTGTG 
CGGAACGGTT ATTCATGTGT GCCTGTAGCT CTTACTGAAA ACCTGGACAT CCGTGTCAAT 
AGCGCCGTCA AGGAGATAAA GTACGGCACC AAAGGTGTGG AAGTAGTGGC TGAAAACTTG 
AAGACATCCA ATTCGCAGAT GACATACAAG GCAGACCTTG TCGTCTGCAC ACTTACTCTG 
GGCGTCCTCA AAGTGGCGGT AGCACATAAG GAGTCACAGC AGAGTAACAC AGTCAAGTTC 
GACCCGCCAT TGCCGGACTG GAAACAGCAG GCCATCAAAA GGCTGGGATT TGGGAATCTC 
AATAAGGTTG TGCTCTGCTT CGATCGCATT TTTTGGGATC CCAACGCTAA TCTCTTCGGA 
CACGTGGGCA GCACGACGGC GAGTAGAGGA GAAATGTTTC TGTTCTGGAG TATAAGCTCA 
TCTCCGGTGT TGCTCGCTCT AGTCGCCGGA ATGGCAGCGA ATCTGGTGGA GAGCGTGACG 
GATGACATCA TAATCGGGCG TTGTATGTCG GTGCTAAAAA ACATCTTCGG GAATACCTCT 
GTTCCACAAC CCAAGGAAAC AGTGGTAACG CGTTGGCGCA GCGATCCCTG GGCCCGTGGG 
TCGTACAGCT ACGTATCCGT TGGTTCCTCG GGCAGTGACT ACGATCTGCT GGCGGCGCCG 
GTCATTCCGC CGTCCAGTAA GGATGCGGAA GGATTGCCAC GGCTATTCTT CGCGGGTGAG 
CATACGATAC GGAATTATCC GGCCACTGTG CATGGAGCGT ACTTGAGCGG ATTGCGCGAG 
GCGGGACGTA TTGCGGATTA CTATCTCGGC TATCCAGAGG GAACACCGCC GGACATTGGC 
TACTCGGTGG CTGAGGCGGC CAATCTGGTG TCCGTGGGAA ATGTTGTCAA GCTGCGCGAC 
CTCTCGCCCA ATCTATCTGA CTCCTCCCCG TCGTCAAAGA AGTCGGAGGA GAATTCAAAC 
TCAAACACTG CCGACTCTAC GGAGCTACAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Su(var)3-3 ( NM_001275171.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262100.1 Drosophila melanogaster suppressor of variegation 3-3, transcript variant C (Su(var)3-3), mRNA.

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CloneID	OFa21119
Clone ID Related Accession (Same CDS sequence)	NM_001275171.1
Accession Version	NM_001275171.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	suppressor of variegation 3-3, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAACCCA CCCAGTTCGG AGGCAGTAGC TCCAAGATGA CCGAGCCAAT TGAGTATGTG 
ACGCTAATTA GCGACGATTC CGATGGTGAG CCCACGCCCA AAAGAAACGT CAATCACCCG 
CCGTCGGCAT TGTCAGCACC CAATCCTGGT CAGAAACAAA AGCACCCGGA TGAGGACTCA 
AATGACGCAC CTGCCACCAG CGATGAGCGA CGCACAAGTA GGCGCAATCG GCCAAAAGTG 
GATTATAGTA ACAGGCCTAG TGGAAGTGGA GACACCGCCT CCAATGACAA GTCAGGATCG 
GCATCCATGG GACCTAACAA CCAGCAAGCA GAGCGTCGCA GCCAAAGCCA AACCCGCAAG 
TCAGAAGCCA ATGCTACCTC CTCCTCCGTG TCCGGACCCA GTGCCGGAAA TTCCAGACCG 
TCCCAGAACG GAGATTCCAA GGACCGGGAT GCCGGCACGC CCACGGTTTT GTCTGGCCAA 
GAGGGCGCCG TCTTCCAATC TCGCCTGCCA TTTAACAAAA TGACACCCAA CGAAGAGGCC 
TGCTTTCCGG ACATCAGTCG CTCGGGCATT CTCGGCCATC GGGTATTCCT CAACATTCGT 
AACAGCCTCT TGCACATGTG GGTGGACAAT CCCAAGATCC AGCTGAGCTT CGAGATCGCC 
CTGAAGAACC TGCCGCCGCC ATTCGACAGC GAGCCCAGTT TGCGACTTAA GCCTATACCC 
GCCAAAAAGC TGGGCAAAGT CATAGTGATT GGGGCAGGGA TCTCCGGCTT AGCAGTGGCA 
CACCAGTTGC AGCAGTTCGG CATGGATGTG ATCGTCTTGG AAGCGCGGGA TCGGGTAGGG 
GGACGGATAT CTACGTTTCG GAAGAACAGC TATATTGCCG ATGTGGGAGC AATGGTGGTT 
ACCGGCGTCT ATGGCAATCC AATGACGATC CTCAGCAAGC AGATCGGTAT GGACCTAGTT 
CCCATCCAGC AGACATGTCC ACTTTACGGC CCAGATGGAA AGCCCGTGCC CAAGGAGAAG 
GATGACGTGA TCGAGAGAGA ATTTAACCGA CTGCTGGAAT CAGCCAGCTA CCTGTCCCAC 
CGCCTTGATT TTAACTACGC CGGCGATTGC CCAGTTTCGC TGGGCGATGC CTTGGAGTGG 
ATAATCAGTA TGCAGGAGAT GCAGGTGATG CACAAACGGG GGCAGCACAT GCAGGAGATC 
ATAGCCACCC AAACGAAGAT CATTGAACAG CGTCGTCGAC TGAAAACCCT TAGAGATACC 
ATCGGCAAAC TAAAGAATGA GCACTTGGCA ATGATCAACC AGCGGAAACC CAAAGGCACG 
GATGGCGATC TGAAATACTG TTACCAAGAG TTTAACATTC GGAACACCCA GATCAAAATG 
GAGGAAACGA TCAGTACGTT TCACGATCTC CATGCCGAGG AAAAGCAGAT GTTAGCCAAA 
CTCCACGAAC TGGAGCAGAA CCGACCTAGC GATGTGTACC TCTCGTCCCG CGACCGCCTG 
ATCTTGGACT GGCATTTTGC CAACTTGGAA TTTGCTAATG CCACCCGACT AAATAACCTG 
TCGTTGAAGC ACTGGGATCA GGACGATGAC TTCGAGTTTA TTGGCCACCA CACAACTGTG 
CGGAACGGTT ATTCATGTGT GCCTGTAGCT CTTACTGAAA ACCTGGACAT CCGTGTCAAT 
AGCGCCGTCA AGGAGATAAA GTACGGCACC AAAGGTGTGG AAGTAGTGGC TGAAAACTTG 
AAGACATCCA ATTCGCAGAT GACATACAAG GCAGACCTTG TCGTCTGCAC ACTTACTCTG 
GGCGTCCTCA AAGTGGCGGT AGCACATAAG GAGTCACAGC AGAGTAACAC AGTCAAGTTC 
GACCCGCCAT TGCCGGACTG GAAACAGCAG GCCATCAAAA GGCTGGGATT TGGGAATCTC 
AATAAGGTTG TGCTCTGCTT CGATCGCATT TTTTGGGATC CCAACGCTAA TCTCTTCGGA 
CACGTGGGCA GCACGACGGC GAGTAGAGGA GAAATGTTTC TGTTCTGGAG TATAAGCTCA 
TCTCCGGTGT TGCTCGCTCT AGTCGCCGGA ATGGCAGCGA ATCTGGTGGA GAGCGTGACG 
GATGACATCA TAATCGGGCG TTGTATGTCG GTGCTAAAAA ACATCTTCGG GAATACCTCT 
GTTCCACAAC CCAAGGAAAC AGTGGTAACG CGTTGGCGCA GCGATCCCTG GGCCCGTGGG 
TCGTACAGCT ACGTATCCGT TGGTTCCTCG GGCAGTGACT ACGATCTGCT GGCGGCGCCG 
GTCATTCCGC CGTCCAGTAA GGATGCGGAA GGATTGCCAC GGCTATTCTT CGCGGGTGAG 
CATACGATAC GGAATTATCC GGCCACTGTG CATGGAGCGT ACTTGAGCGG ATTGCGCGAG 
GCGGGACGTA TTGCGGATTA CTATCTCGGC TATCCAGAGG GAACACCGCC GGACATTGGC 
TACTCGGTGG CTGAGGCGGC CAATCTGGTG TCCGTGGGAA ATGTTGTCAA GCTGCGCGAC 
CTCTCGCCCA ATCTATCTGA CTCCTCCCCG TCGTCAAAGA AGTCGGAGGA GAATTCAAAC 
TCAAACACTG CCGACTCTAC GGAGCTACAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262100.1
CDS	370..2982
Translation	MKPTQFGGSSSKMTEPIEYVTLISDDSDGEPTPKRNVNHPPSALSAPNPGQKQKHPDEDSNDAPATSDERRTSRRNRPKVDYSNRPSGSGDTASNDKSGSASMGPNNQQAERRSQSQTRKSEANATSSSVSGPSAGNSRPSQNGDSKDRDAGTPTVLSGQEGAVFQSRLPFNKMTPNEEACFPDISRSGILGHRVFLNIRNSLLHMWVDNPKIQLSFEIALKNLPPPFDSEPSLRLKPIPAKKLGKVIVIGAGISGLAVAHQLQQFGMDVIVLEARDRVGGRISTFRKNSYIADVGAMVVTGVYGNPMTILSKQIGMDLVPIQQTCPLYGPDGKPVPKEKDDVIEREFNRLLESASYLSHRLDFNYAGDCPVSLGDALEWIISMQEMQVMHKRGQHMQEIIATQTKIIEQRRRLKTLRDTIGKLKNEHLAMINQRKPKGTDGDLKYCYQEFNIRNTQIKMEETISTFHDLHAEEKQMLAKLHELEQNRPSDVYLSSRDRLILDWHFANLEFANATRLNNLSLKHWDQDDDFEFIGHHTTVRNGYSCVPVALTENLDIRVNSAVKEIKYGTKGVEVVAENLKTSNSQMTYKADLVVCTLTLGVLKVAVAHKESQQSNTVKFDPPLPDWKQQAIKRLGFGNLNKVVLCFDRIFWDPNANLFGHVGSTTASRGEMFLFWSISSSPVLLALVAGMAANLVESVTDDIIIGRCMSVLKNIFGNTSVPQPKETVVTRWRSDPWARGSYSYVSVGSSGSDYDLLAAPVIPPSSKDAEGLPRLFFAGEHTIRNYPATVHGAYLSGLREAGRIADYYLGYPEGTPPDIGYSVAEAANLVSVGNVVKLRDLSPNLSDSSPSSKKSEENSNSNTADSTELQ

Target ORF information:

RefSeq Version	NM_001275171.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster suppressor of variegation 3-3, transcript variant C (Su(var)3-3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275171.1

ORF Insert Sequence:

ATGAAACCCA CCCAGTTCGG AGGCAGTAGC TCCAAGATGA CCGAGCCAAT TGAGTATGTG 
ACGCTAATTA GCGACGATTC CGATGGTGAG CCCACGCCCA AAAGAAACGT CAATCACCCG 
CCGTCGGCAT TGTCAGCACC CAATCCTGGT CAGAAACAAA AGCACCCGGA TGAGGACTCA 
AATGACGCAC CTGCCACCAG CGATGAGCGA CGCACAAGTA GGCGCAATCG GCCAAAAGTG 
GATTATAGTA ACAGGCCTAG TGGAAGTGGA GACACCGCCT CCAATGACAA GTCAGGATCG 
GCATCCATGG GACCTAACAA CCAGCAAGCA GAGCGTCGCA GCCAAAGCCA AACCCGCAAG 
TCAGAAGCCA ATGCTACCTC CTCCTCCGTG TCCGGACCCA GTGCCGGAAA TTCCAGACCG 
TCCCAGAACG GAGATTCCAA GGACCGGGAT GCCGGCACGC CCACGGTTTT GTCTGGCCAA 
GAGGGCGCCG TCTTCCAATC TCGCCTGCCA TTTAACAAAA TGACACCCAA CGAAGAGGCC 
TGCTTTCCGG ACATCAGTCG CTCGGGCATT CTCGGCCATC GGGTATTCCT CAACATTCGT 
AACAGCCTCT TGCACATGTG GGTGGACAAT CCCAAGATCC AGCTGAGCTT CGAGATCGCC 
CTGAAGAACC TGCCGCCGCC ATTCGACAGC GAGCCCAGTT TGCGACTTAA GCCTATACCC 
GCCAAAAAGC TGGGCAAAGT CATAGTGATT GGGGCAGGGA TCTCCGGCTT AGCAGTGGCA 
CACCAGTTGC AGCAGTTCGG CATGGATGTG ATCGTCTTGG AAGCGCGGGA TCGGGTAGGG 
GGACGGATAT CTACGTTTCG GAAGAACAGC TATATTGCCG ATGTGGGAGC AATGGTGGTT 
ACCGGCGTCT ATGGCAATCC AATGACGATC CTCAGCAAGC AGATCGGTAT GGACCTAGTT 
CCCATCCAGC AGACATGTCC ACTTTACGGC CCAGATGGAA AGCCCGTGCC CAAGGAGAAG 
GATGACGTGA TCGAGAGAGA ATTTAACCGA CTGCTGGAAT CAGCCAGCTA CCTGTCCCAC 
CGCCTTGATT TTAACTACGC CGGCGATTGC CCAGTTTCGC TGGGCGATGC CTTGGAGTGG 
ATAATCAGTA TGCAGGAGAT GCAGGTGATG CACAAACGGG GGCAGCACAT GCAGGAGATC 
ATAGCCACCC AAACGAAGAT CATTGAACAG CGTCGTCGAC TGAAAACCCT TAGAGATACC 
ATCGGCAAAC TAAAGAATGA GCACTTGGCA ATGATCAACC AGCGGAAACC CAAAGGCACG 
GATGGCGATC TGAAATACTG TTACCAAGAG TTTAACATTC GGAACACCCA GATCAAAATG 
GAGGAAACGA TCAGTACGTT TCACGATCTC CATGCCGAGG AAAAGCAGAT GTTAGCCAAA 
CTCCACGAAC TGGAGCAGAA CCGACCTAGC GATGTGTACC TCTCGTCCCG CGACCGCCTG 
ATCTTGGACT GGCATTTTGC CAACTTGGAA TTTGCTAATG CCACCCGACT AAATAACCTG 
TCGTTGAAGC ACTGGGATCA GGACGATGAC TTCGAGTTTA TTGGCCACCA CACAACTGTG 
CGGAACGGTT ATTCATGTGT GCCTGTAGCT CTTACTGAAA ACCTGGACAT CCGTGTCAAT 
AGCGCCGTCA AGGAGATAAA GTACGGCACC AAAGGTGTGG AAGTAGTGGC TGAAAACTTG 
AAGACATCCA ATTCGCAGAT GACATACAAG GCAGACCTTG TCGTCTGCAC ACTTACTCTG 
GGCGTCCTCA AAGTGGCGGT AGCACATAAG GAGTCACAGC AGAGTAACAC AGTCAAGTTC 
GACCCGCCAT TGCCGGACTG GAAACAGCAG GCCATCAAAA GGCTGGGATT TGGGAATCTC 
AATAAGGTTG TGCTCTGCTT CGATCGCATT TTTTGGGATC CCAACGCTAA TCTCTTCGGA 
CACGTGGGCA GCACGACGGC GAGTAGAGGA GAAATGTTTC TGTTCTGGAG TATAAGCTCA 
TCTCCGGTGT TGCTCGCTCT AGTCGCCGGA ATGGCAGCGA ATCTGGTGGA GAGCGTGACG 
GATGACATCA TAATCGGGCG TTGTATGTCG GTGCTAAAAA ACATCTTCGG GAATACCTCT 
GTTCCACAAC CCAAGGAAAC AGTGGTAACG CGTTGGCGCA GCGATCCCTG GGCCCGTGGG 
TCGTACAGCT ACGTATCCGT TGGTTCCTCG GGCAGTGACT ACGATCTGCT GGCGGCGCCG 
GTCATTCCGC CGTCCAGTAA GGATGCGGAA GGATTGCCAC GGCTATTCTT CGCGGGTGAG 
CATACGATAC GGAATTATCC GGCCACTGTG CATGGAGCGT ACTTGAGCGG ATTGCGCGAG 
GCGGGACGTA TTGCGGATTA CTATCTCGGC TATCCAGAGG GAACACCGCC GGACATTGGC 
TACTCGGTGG CTGAGGCGGC CAATCTGGTG TCCGTGGGAA ATGTTGTCAA GCTGCGCGAC 
CTCTCGCCCA ATCTATCTGA CTCCTCCCCG TCGTCAAAGA AGTCGGAGGA GAATTCAAAC 
TCAAACACTG CCGACTCTAC GGAGCTACAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Su(var)3-3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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