Tom20 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tom20
Entrez Gene ID	40189
Full Name	Translocase of outer membrane 20
Synonyms	CG7654,Dmel\CG7654,TOM 20,TOM20,anon-WO0118547.225,dtom20
General protein information	Preferred Names Translocase of outer membrane 20 Names CG7654 gene product from transcript CG7654-RB CG7654-PA CG7654-PB Tom20-PA Tom20-PB translocase of outer membrane 20
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 76E1-76E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_140912.4	NP_649169.3	translocase of outer membrane 20, isoform A
NM_001275156.1	NP_001262085.1	translocase of outer membrane 20, isoform B

Pathways from BioSystems

Homologies

	TOMM20	XP_001102478.1
Canis lupus familiaris (dog)	TOMM20	XP_003434490.1
Rattus norvegicus (Norway rat)	Tomm20	NP_690918.1
Gallus gallus (chicken)	TOMM20	XP_423972.3
Xenopus tropicalis (tropical clawed frog)	tomm20	NP_001005024.1
Homo sapiens (human)	TOMM20	NP_055580.1
Danio rerio (zebrafish)	tomm20b	NP_001002698.1
Caenorhabditis elegans (roundworm)	tomm-20	NP_506092.1
Bos taurus (cattle)	TOMM20	NP_001092653.1
Mus musculus (house mouse)	Tomm20	NP_077176.1
Drosophila melanogaster (fruit fly)	Tom20	NP_001262085.1
Pan troglodytes (chimpanzee)	TOMM20	XP_001153572.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012339	XP_320211.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tom20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tom20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa19047	NM_140912.4 Latest version!	Drosophila melanogaster translocase of outer membrane 20, transcript variant A (Tom20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa19047	NM_001275156.1 Latest version!	Drosophila melanogaster translocase of outer membrane 20, transcript variant B (Tom20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tom20 ( NM_140912.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649169.3 Drosophila melanogaster translocase of outer membrane 20, transcript variant A (Tom20), mRNA.

Price & Availability↑

CloneID	OFa19047
Clone ID Related Accession (Same CDS sequence)	NM_140912.4 , NM_001275156.1
Accession Version	NM_140912.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	translocase of outer membrane 20, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140912.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTGAAA TGAACAAAAC TGCAATCGGC ATTGCAGCGG GAGTAGCTGG AACTCTGTTT 
ATTGGATACT GCATCTACTT CGACAAGAAG CGCCGCAGCG ATCCCGAGTA CAAGAAGAAA 
GTCCGTGAGC GTCGCCGTCG CAACAAGAAA ACCGGCACTG CCAAGTCTGG AGTTCCCAAT 
CTGAACGACC ATGAAGCCAT CGAAAGATAT TTCCTTCAAG AAATCCAGCT GGGCGAGACC 
CTGATCGCCC GCGGAGATTT CGAGAGTGGC GTGGAGCACT TGGCGAATGC CATCGTCGTG 
TGTGGCCAGC CGGCTCGTCT GCTGCAGGTG CTGCAATCCT CCCTTCCAGC TCAAGTATTC 
GCGATGCTGA TCGTCAAGAT GCAGGAGTTC GGCAATCGAG CGGCCGAGGG CAACGACGGG 
CCAATCGTTT TGGGGCAGAG CTCGGAGCAA CAACTGGACG GAGCCAAAAT TATAGAGTGT 
TCATCTGGAA ACGCAAGTAT CGACGACCTC GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649169.3
CDS	80..595
Translation	MIEMNKTAIGIAAGVAGTLFIGYCIYFDKKRRSDPEYKKKVRERRRRNKKTGTAKSGVPNLNDHEAIERYFLQEIQLGETLIARGDFESGVEHLANAIVVCGQPARLLQVLQSSLPAQVFAMLIVKMQEFGNRAAEGNDGPIVLGQSSEQQLDGAKIIECSSGNASIDDLE

Target ORF information:

RefSeq Version	NM_140912.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster translocase of outer membrane 20, transcript variant A (Tom20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140912.4

ORF Insert Sequence:

ATGATTGAAA TGAACAAAAC TGCAATCGGC ATTGCAGCGG GAGTAGCTGG AACTCTGTTT 
ATTGGATACT GCATCTACTT CGACAAGAAG CGCCGCAGCG ATCCCGAGTA CAAGAAGAAA 
GTCCGTGAGC GTCGCCGTCG CAACAAGAAA ACCGGCACTG CCAAGTCTGG AGTTCCCAAT 
CTGAACGACC ATGAAGCCAT CGAAAGATAT TTCCTTCAAG AAATCCAGCT GGGCGAGACC 
CTGATCGCCC GCGGAGATTT CGAGAGTGGC GTGGAGCACT TGGCGAATGC CATCGTCGTG 
TGTGGCCAGC CGGCTCGTCT GCTGCAGGTG CTGCAATCCT CCCTTCCAGC TCAAGTATTC 
GCGATGCTGA TCGTCAAGAT GCAGGAGTTC GGCAATCGAG CGGCCGAGGG CAACGACGGG 
CCAATCGTTT TGGGGCAGAG CTCGGAGCAA CAACTGGACG GAGCCAAAAT TATAGAGTGT 
TCATCTGGAA ACGCAAGTAT CGACGACCTC GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tom20 ( NM_001275156.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262085.1 Drosophila melanogaster translocase of outer membrane 20, transcript variant B (Tom20), mRNA.

Price & Availability↑

CloneID	OFa19047
Clone ID Related Accession (Same CDS sequence)	NM_140912.4 , NM_001275156.1
Accession Version	NM_001275156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	translocase of outer membrane 20, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTGAAA TGAACAAAAC TGCAATCGGC ATTGCAGCGG GAGTAGCTGG AACTCTGTTT 
ATTGGATACT GCATCTACTT CGACAAGAAG CGCCGCAGCG ATCCCGAGTA CAAGAAGAAA 
GTCCGTGAGC GTCGCCGTCG CAACAAGAAA ACCGGCACTG CCAAGTCTGG AGTTCCCAAT 
CTGAACGACC ATGAAGCCAT CGAAAGATAT TTCCTTCAAG AAATCCAGCT GGGCGAGACC 
CTGATCGCCC GCGGAGATTT CGAGAGTGGC GTGGAGCACT TGGCGAATGC CATCGTCGTG 
TGTGGCCAGC CGGCTCGTCT GCTGCAGGTG CTGCAATCCT CCCTTCCAGC TCAAGTATTC 
GCGATGCTGA TCGTCAAGAT GCAGGAGTTC GGCAATCGAG CGGCCGAGGG CAACGACGGG 
CCAATCGTTT TGGGGCAGAG CTCGGAGCAA CAACTGGACG GAGCCAAAAT TATAGAGTGT 
TCATCTGGAA ACGCAAGTAT CGACGACCTC GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262085.1
CDS	206..721
Translation	MIEMNKTAIGIAAGVAGTLFIGYCIYFDKKRRSDPEYKKKVRERRRRNKKTGTAKSGVPNLNDHEAIERYFLQEIQLGETLIARGDFESGVEHLANAIVVCGQPARLLQVLQSSLPAQVFAMLIVKMQEFGNRAAEGNDGPIVLGQSSEQQLDGAKIIECSSGNASIDDLE

Target ORF information:

RefSeq Version	NM_001275156.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster translocase of outer membrane 20, transcript variant B (Tom20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275156.1

ORF Insert Sequence:

ATGATTGAAA TGAACAAAAC TGCAATCGGC ATTGCAGCGG GAGTAGCTGG AACTCTGTTT 
ATTGGATACT GCATCTACTT CGACAAGAAG CGCCGCAGCG ATCCCGAGTA CAAGAAGAAA 
GTCCGTGAGC GTCGCCGTCG CAACAAGAAA ACCGGCACTG CCAAGTCTGG AGTTCCCAAT 
CTGAACGACC ATGAAGCCAT CGAAAGATAT TTCCTTCAAG AAATCCAGCT GGGCGAGACC 
CTGATCGCCC GCGGAGATTT CGAGAGTGGC GTGGAGCACT TGGCGAATGC CATCGTCGTG 
TGTGGCCAGC CGGCTCGTCT GCTGCAGGTG CTGCAATCCT CCCTTCCAGC TCAAGTATTC 
GCGATGCTGA TCGTCAAGAT GCAGGAGTTC GGCAATCGAG CGGCCGAGGG CAACGACGGG 
CCAATCGTTT TGGGGCAGAG CTCGGAGCAA CAACTGGACG GAGCCAAAAT TATAGAGTGT 
TCATCTGGAA ACGCAAGTAT CGACGACCTC GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Tom20 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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