Mi-2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mi-2
Entrez Gene ID	40170
Full Name	CG8103 gene product from transcript CG8103-RA
Synonyms	0006/06,0854/01,CG8103,Chd4,Dm-Mi-2,Dmel\CG8103,M-i2,MI-2,MI2,Mi 2,Mi2,Pha,dMI-2,dMi,dMi-2,dMi2,hip76,l(3)01058,l(3)76BDe,l(3)A154.3M3,l(3)L1243,l(3)S000606,l(3)S085401,l(3)j3D4,pha
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 76D3-76D4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275149.1	NP_001262078.1	Mi-2, isoform D
NM_001170005.2	NP_001163476.1	Mi-2, isoform C
NM_001014591.3	NP_001014591.1	Mi-2, isoform B
NM_140897.4	NP_649154.2	Mi-2, isoform A

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CHD5	XP_002694217.3
Mus musculus (house mouse)	Chd5	NP_001074845.1
Drosophila melanogaster (fruit fly)	Mi-2	NP_001014591.1
Homo sapiens (human)	CHD5	NP_056372.1
Rattus norvegicus (Norway rat)	Chd5	NP_001258155.1
Gallus gallus (chicken)	CHD5	XP_003642567.2
Danio rerio (zebrafish)	chd5	XP_003199545.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012009	XP_320523.4
Canis lupus familiaris (dog)	CHD5	XP_005620504.1
Xenopus tropicalis (tropical clawed frog)	chd5	XP_002937591.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results suggest that dMi-2 distribution over active heat shock genes are determined by transcriptional activity.
Title: Recruitment of the ATP-dependent chromatin remodeler dMi-2 to the transcribed region of active heat shock genes.

dMi-2 is an important regulator of both chromosome condensation and cohesin binding in interphase cells.
Title: The Drosophila MI-2 chromatin-remodeling factor regulates higher-order chromatin structure and cohesin dynamics in vivo.

Results suggest that Drosophila Mi-2/NuRD complex functions in neuronal differentiation through the repression of proneural gene expression by chromatin remodeling and histone deacetylation.
Title: Mi-2 chromatin remodeling factor functions in sensory organ development through proneural gene repression in Drosophila.

results reveal a potential mechanism for regulation of the dMi-2 enzyme and point toward CK2 phosphorylation as a common feature of CHD family ATPases
Title: dMi-2 chromatin binding and remodeling activities are regulated by dCK2 phosphorylation.

Drosophila Mi-2 negatively regulates dDREF by inhibiting its DNA-binding activity.
Title: Drosophila Mi-2 negatively regulates dDREF by inhibiting its DNA-binding activity.

The following Mi-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mi-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19027	NM_140897.4 Latest version!	Drosophila melanogaster Mi-2, transcript variant A (Mi-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa19027	NM_001170005.2 Latest version!	Drosophila melanogaster Mi-2, transcript variant C (Mi-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa19028	NM_001014591.3 Latest version!	Drosophila melanogaster Mi-2, transcript variant B (Mi-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa21108	NM_001275149.1 Latest version!	Drosophila melanogaster Mi-2, transcript variant D (Mi-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mi-2 ( NM_001170005.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163476.1 Drosophila melanogaster Mi-2, transcript variant C (Mi-2), mRNA.

Price & Availability↑

CloneID	OFa19027
Clone ID Related Accession (Same CDS sequence)	NM_001170005.2 , NM_140897.4
Accession Version	NM_001170005.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Mi-2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001170005.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATCGG AGGAAGAGAA TGACGATAAT TTCCAGGAAG AAGAAGAGGC CCAGGAGGAC 
AACGCACCTG CGGCGGAGCT GTCCAACGAT TCCGACGCCC CTCTGAAGCC GAATAACGAC 
GAAGACGACG ACTACGATCC GGAGGACAGT CGCAGGAAGA AGAAGGGCAA GAAGCGCAAG 
ACCAGGAAGG GCGAAGAGAA GGGTCGCAAG AAGAAGAAGC GCAAGAAGAA CGAGAGCGAA 
GAGGACAGCG ACTTTGTCCA GCACGATGAG GAGGTGGAGT ACCCCAGCAC CTCCAAGCGC 
GGTCGCAAGC GCAAGGAGGA GAAACAGGCC GCCAAGGAGA AGGAGTCGGC ATCATCCGGA 
ATGCCATCTG TGGAAGACGT GTGCTCAGCC TTTAGCGTCT GCAATGTGGA GATCGAGTAC 
AGTGAGGAGG AGCTGCAGAG CCTAACGACC TACAAGGCTT TCATGCATCA TGTTCGTCCG 
ATTCTGCAGA AGGAAAACCC CAAGATTGCG GCACCAAAGC TAGTGATGCT GGTGGCAGCC 
AAGTGGCGTG AGTTCTGCGA GAGCAATCCG CACATCCAGC AGGAGGGTGG TGCCGCCGGC 
TCCGGTGGAT CCGCCGGACA GGCACGCAGT GTGACAGGCG ATGAGCCGGA GGAACCTCGA 
TCATCGCGTT CCTCGCGCAA CGAGAAACCG GATGACATTT ACGAGGAGGC CGTCGAGGAG 
GAGGAAGAAG AGGAGGAGGA GGAGAAGAAA CCTCGTCGTA AGCGCAGTGG ACGTGGCAAG 
AAGGGGCGTC GTCCCTCCGG CAAGGTGCCC ACTCTTAAGA TTAAGCTGCT CGGCAAGCGC 
AAACGCGATA GTTCGGATGA GGAGCAAGAT GCTAGCGGTG CATCGGAACG CGATTCGGAT 
CTTGAGTTTG AACGGATGCT TCAGAAATCG GATGACAGTG CCGATGAAAA GGAGGCTCCT 
GTCTCCTCTA AGGCGGACAA CTCAGCACCC GCTGCCCAGG ACGATGGATC AGGAGCTCCA 
GTGGTGCGCA AGAAGGCCAA GACCAAGATC GGCAACAAGT TCAAGAAGAA GAATAAACTC 
AAGAAAACGA AGAACTTCCC GGAGGGCGAA GATGGCGAGC ATGAGCACCA GGACTACTGC 
GAGGTGTGCC AGCAAGGAGG TGAGATCATC CTGTGCGACA CCTGCCCTCG GGCATATCAC 
TTGGTGTGCC TGGAGCCAGA ACTCGATGAA CCGCCAGAGG GCAAGTGGTC GTGTCCGCAC 
TGCGAGGCTG ACGGAGGTGC TGCTGAGGAA GAGGACGATG ATGAGCACCA GGAATTCTGC 
CGCGTGTGCA AGGACGGTGG CGAGTTGCTG TGCTGCGACT CATGTCCCTC CGCCTATCAC 
ACATTCTGCT TGAATCCGCC TCTGGACACT ATACCCGATG GCGATTGGCG CTGTCCTCGT 
TGCAGCTGCC CTCCGCTCAC TGGCAAGGCT GAGAAGATCA TCACCTGGCG ATGGGCACAG 
CGTAGCAATG ACGACGGTCC CTCCACCTCG AAGGGATCAA AGAACAGTAA CTCCCGGGTG 
CGCGAGTACT TTATCAAGTG GCACAACATG TCCTACTGGC ATTGCGAATG GGTGCCCGAA 
GTGCAACTGG ACGTCCATCA TCCGCTCATG ATTCGTTCGT TCCAGCGCAA GTACGACATG 
GAAGAACCGC CCAAGTTCGA GGAGTCGCTG GATGAGGCGG ACACTCGTTA CAAGCGTATT 
CAGCGACACA AGGACAAGGT TGGCATGAAG GCAAACGATG ATGCCGAAGT GCTGGAGGAG 
CGGTTCTACA AGAATGGCGT CAAGCCAGAG TGGCTCATTG TTCAGCGCGT AATCAACCAT 
CGTACGGCAA GAGATGGCAG CACCATGTAT CTGGTGAAGT GGCGCGAGTT GCCCTATGAC 
AAGTCCACCT GGGAAGAAGA GGGTGATGAC ATACAGGGCC TGCGGCAAGC CATCGATTAC 
TATCAGGATC TGCGTGCGGT TTGCACCTCG GAGACAACTC AGTCGCGCAG CAAAAAGAGC 
AAGAAGGGTC GCAAGTCCAA GCTCAAGGTC GAGGACGACG AGGATCGCCC GGTCAAGCAC 
TACACACCGC CGCCGGAAAA GCCAACTACT GATCTGAAGA AGAAGTATGA GGATCAGCCG 
GCTTTCTTGG AGGGCACTGG CATGCAATTG CATCCCTACC AGATCGAGGG CATTAACTGG 
TTGCGCTACA GCTGGGGCCA GGGCATCGAC ACCATCCTGG CCGACGAGAT GGGTCTGGGT 
AAGACCATTC AGACGGTCAC CTTCCTGTAC TCTTTGTACA AGGAGGGCCA TTGCCGCGGA 
CCCTTCCTCG TGGCCGTGCC ACTCTCGACG CTAGTGAACT GGGAGCGTGA GTTTGAGCTG 
TGGGCTCCGG ACTTTTACTG CATCACGTAT ATCGGTGACA AGGACTCGAG AGCGGTTATA 
CGGGAGAACG AGCTGAGCTT CGAGGAGGGC GCCATTCGTG GTAGCAAGGT TTCGCGTTTG 
CGGACCACCC AGTATAAATT CAACGTACTG CTGACTAGCT ACGAGCTGAT CTCCATGGAC 
GCTGCTTGCC TTGGCAGCAT TGACTGGGCC GTGCTCGTGG TGGACGAGGC CCATCGCTTG 
AAGAGCAACC AGAGCAAGTT CTTCCGTATC CTGAACAGCT ACACTATTGC CTACAAGCTT 
CTGCTCACCG GCACACCGCT GCAGAACAAT CTCGAGGAGC TGTTCCATCT GCTCAACTTC 
TTGAGCCGCG ACAAGTTCAA TGATCTGCAG GCCTTCCAGG GCGAATTCGC TGACGTTTCG 
AAGGAGGAGC AGGTTAAGCG TCTGCACGAG ATGCTTGGAC CGCACATGCT GCGTCGTTTG 
AAGACGGATG TGCTGAAGAA CATGCCGTCC AAGTCTGAGT TTATCGTGCG CGTCGAGCTG 
TCGGCCATGC AGAAAAAGTT CTACAAGTTC ATCTTGACCA AGAACTACGA GGCTTTGAAT 
TCGAAGAGCG GTGGTGGCTC TTGCTCACTG ATCAACATTA TGATGGACCT AAAGAAGTGC 
TGCAACCATC CGTATCTCTT CCCCTCGGCC GCCGAGGAGG CAACCACCGC TGCCGGTGGT 
CTCTACGAGA TTAACTCGCT GACCAAGGCT GCTGGCAAGC TGGTCCTGCT ATCCAAGATG 
CTGAAGCAGC TGAAGGCACA AAACCATCGT GTCTTGATTT TCTCCCAGAT GACCAAGATG 
TTGGACATTC TCGAGGACTT CCTCGAGGGC GAGCAGTACA AGTACGAACG TATTGACGGT 
GGAATCACAG GAACATTGCG TCAGGAAGCC ATTGATAGGT TCAATGCACC CGGAGCGCAG 
CAGTTTGTCT TCTTGCTGAG TACTCGAGCC GGAGGATTGG GTATTAATTT GGCCACGGCC 
GACACTGTCA TTATTTATGA CTCCGATTGG AATCCCCACA ACGATATTCA GGCCTTCTCC 
CGAGCCCATC GTATTGGCCA GGCTAACAAG GTGATGATCT ATCGATTTGT TACTCGAAAT 
TCTGTGGAGG AGCGCGTTAC CCAGGTGGCC AAGCGTAAGA TGATGTTGAC TCATCTTGTG 
GTCCGTCCGG GAATGGGCGG TAAAGGCGCT AACTTTACAA AGCAAGAACT GGACGATATC 
CTTCGTTTCG GTACCGAGGA TCTGTTCAAG GAGGATGACA AGGAGGAAGC TATCCACTAT 
GACGACAAGG CGGTGGCCGA GCTGCTCGAC AGAACCAACC GCGGTATCGA GGAGAAGGAG 
TCCTGGGCCA ACGAGTATCT GTCCTCGTTT AAGGTGGCTT CGTACGCCAC TAAAGAAGAG 
GAGGAGGAGG AAGAGACGGA GATTATTAAA CAGGATGCCG AGAACTCTGA TCCGGCGTAC 
TGGGTGAAGC TGCTGCGGCA TCACTATGAA CAGCACCAGG AAGATGTGGG TCGCAGCCTG 
GGCAAGGGCA AGCGCGTCCG CAAACAGGTC AACTACACGG ATGGCGGCGT TGTGGCGGCT 
GATACCACGC GGGATGATTC CAACTGGCAG GACAACGGCA GCGAGTATAA CTCCGAGTAT 
TCAGCTGGCT CCGATGAGGA TGGCGGTGAC GATGACTTCG ATGACCAGAA TGGCGCAGAG 
AGAAAGGCCA AGCGGCGATT GGAGCGTCGC GACGATCGTC CATTGCCTCC ACTGTTGGCC 
CGCGTGGGTG GAAACATCGA GGTGCTCGGT TTTAATGCCC GTCAACGCAA GAGCTTCCTC 
AATGCCATTA TGCGGTACGG AATGCCGCCG CAAGATGCCT TCAACTCGCA ATGGCTAGTC 
CGGGATTTGC GTGGTAAATC GGAGCGCAAT TTTAAGGCGT ATGTTTCTCT GTTTATGCGG 
CATCTTTGCG AGCCGGGAGC GGACAATGCG GAAACCTTTG CCGATGGTGT GCCGCGCGAA 
GGACTATCCC GCCAGCATGT GCTCACCCGG ATCGGAGTGA TGTCGCTAAT TCGAAAGAAG 
GTACAGGAAT TCGAGCATAT TAACGGATAC TACAGCATGC CGGAGCTCAT TCTCAAGCCG 
TGCGAGCCCG TACGATCAGC CCTGAAACAG GATGTGGCGG CACTTGAAGC TCCGCCTACC 
GGTGGCAATG TCGACAAGAG CGCTACTACT AGCAATAGCG TCACTCCAGC CACCAGTGCG 
GCTCCCAGTC CAGCGCCAGC CTCTGAAAAA GGTGAAGACA AAGACAAGGA CTCCGAAAAG 
GAGAAGGATA AGACCTCGGC GGAGAAGAGC GAGGTGAAGC AAGAACAAGA GGCTGAAGAG 
GACAAGAAGC CTGGGGATGT CAAGCAGGAG AACCCTGTGG AAGAAGCTGC CGGCGACACA 
AAGCCCAGCG ACGCGGAGGT CAAAACCGAG GTGGCTAAAA CAGAACCCAA GGAAGAGACC 
AAGGACCCGG AGGTAAAGGA GGAGCCCAAG ACCGAAGAGA AGGAAAAAGA AAAGGTGGAT 
GACAAGAAAC CAATACCACC GACGACAGTA ATTGACGACG ACGATGACGA TGTGATGATT 
GTCAAGGAGG ACGGTGAGCT AGAAAAGCCC AGTGCCAGTT CACCCAAGGA TCAAAAGGCA 
GTCGCCGCTG CTACGTCTGC TGCAACTGGA GCCACTGGCA AGGGAGCGGA GGACAGCTTG 
GAGGTGCTAA AGCGCAAATT TATGTTCAAC ATCGCCGACG GCGGGTTCAC CGAACTGCAC 
ACTTTGTGGC TCAACGAGGA GAAGGCTGCC GTTCCTGGGC GAGAATACGA GATCTGGCAC 
CGTCGCCACG ACTACTGGCT GTTGGCCGGA ATTGTGACCC ATGGCTATGG CCGTTGGCAG 
GATATACAAA ACGATATCCG TTTCGCGATC ATAAACGAAC CTTTCAAGAT GGACGTTGGT 
AAGGGCAACT TTCTGGAAAT CAAAAACAAG TTCCTGGCCC GGCGTTTTAA GTTGCTGGAG 
CAGGCGCTGG TCATCGAGGA GCAGCTGCGG CGAGCGGCCT ACCTTAACCT CGCCCAGGAC 
CCCAGCCACC CGGCAATGTC GCTAAACGCC CGTTTCGCAG AGGTCGAATG CCTGGCTGAA 
TCCCATCAGC ATCTGAGCAA GGAATCGCTG GCCGGTAACA AGCCCGCAAA CGCTGTTCTG 
CACAAGGTGC TCAACCAGCT CGAAGAGCTG CTCTCGGACA TGAAGAGCGA TGTGTCCCGG 
CTGCCAGCTA CACTGGCTCG CATCCCACCT GTTGCGCAAC GGCTTCAGAT GTCCGAGAGA 
TCGATTCTCT CCCGACTGGC GGCCACCGCC GGAAATGCCT CCAATGCAGC TCAATTGATG 
GCACAATTCC CGGCTGGATT CCAGGGCACA ACATTGCCAG CATTCACGAG CGGACCGGCT 
GGCAACTTTG CCAACTTTCG GCCACAGTTC TCGGTGCCCG GCCAGCTATC GAATAATTCC 
GGCGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163476.1
CDS	137..6085
Translation	MASEEENDDNFQEEEEAQEDNAPAAELSNDSDAPLKPNNDEDDDYDPEDSRRKKKGKKRKTRKGEEKGRKKKKRKKNESEEDSDFVQHDEEVEYPSTSKRGRKRKEEKQAAKEKESASSGMPSVEDVCSAFSVCNVEIEYSEEELQSLTTYKAFMHHVRPILQKENPKIAAPKLVMLVAAKWREFCESNPHIQQEGGAAGSGGSAGQARSVTGDEPEEPRSSRSSRNEKPDDIYEEAVEEEEEEEEEEKKPRRKRSGRGKKGRRPSGKVPTLKIKLLGKRKRDSSDEEQDASGASERDSDLEFERMLQKSDDSADEKEAPVSSKADNSAPAAQDDGSGAPVVRKKAKTKIGNKFKKKNKLKKTKNFPEGEDGEHEHQDYCEVCQQGGEIILCDTCPRAYHLVCLEPELDEPPEGKWSCPHCEADGGAAEEEDDDEHQEFCRVCKDGGELLCCDSCPSAYHTFCLNPPLDTIPDGDWRCPRCSCPPLTGKAEKIITWRWAQRSNDDGPSTSKGSKNSNSRVREYFIKWHNMSYWHCEWVPEVQLDVHHPLMIRSFQRKYDMEEPPKFEESLDEADTRYKRIQRHKDKVGMKANDDAEVLEERFYKNGVKPEWLIVQRVINHRTARDGSTMYLVKWRELPYDKSTWEEEGDDIQGLRQAIDYYQDLRAVCTSETTQSRSKKSKKGRKSKLKVEDDEDRPVKHYTPPPEKPTTDLKKKYEDQPAFLEGTGMQLHPYQIEGINWLRYSWGQGIDTILADEMGLGKTIQTVTFLYSLYKEGHCRGPFLVAVPLSTLVNWEREFELWAPDFYCITYIGDKDSRAVIRENELSFEEGAIRGSKVSRLRTTQYKFNVLLTSYELISMDAACLGSIDWAVLVVDEAHRLKSNQSKFFRILNSYTIAYKLLLTGTPLQNNLEELFHLLNFLSRDKFNDLQAFQGEFADVSKEEQVKRLHEMLGPHMLRRLKTDVLKNMPSKSEFIVRVELSAMQKKFYKFILTKNYEALNSKSGGGSCSLINIMMDLKKCCNHPYLFPSAAEEATTAAGGLYEINSLTKAAGKLVLLSKMLKQLKAQNHRVLIFSQMTKMLDILEDFLEGEQYKYERIDGGITGTLRQEAIDRFNAPGAQQFVFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQANKVMIYRFVTRNSVEERVTQVAKRKMMLTHLVVRPGMGGKGANFTKQELDDILRFGTEDLFKEDDKEEAIHYDDKAVAELLDRTNRGIEEKESWANEYLSSFKVASYATKEEEEEEETEIIKQDAENSDPAYWVKLLRHHYEQHQEDVGRSLGKGKRVRKQVNYTDGGVVAADTTRDDSNWQDNGSEYNSEYSAGSDEDGGDDDFDDQNGAERKAKRRLERRDDRPLPPLLARVGGNIEVLGFNARQRKSFLNAIMRYGMPPQDAFNSQWLVRDLRGKSERNFKAYVSLFMRHLCEPGADNAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHINGYYSMPELILKPCEPVRSALKQDVAALEAPPTGGNVDKSATTSNSVTPATSAAPSPAPASEKGEDKDKDSEKEKDKTSAEKSEVKQEQEAEEDKKPGDVKQENPVEEAAGDTKPSDAEVKTEVAKTEPKEETKDPEVKEEPKTEEKEKEKVDDKKPIPPTTVIDDDDDDVMIVKEDGELEKPSASSPKDQKAVAAATSAATGATGKGAEDSLEVLKRKFMFNIADGGFTELHTLWLNEEKAAVPGREYEIWHRRHDYWLLAGIVTHGYGRWQDIQNDIRFAIINEPFKMDVGKGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNLAQDPSHPAMSLNARFAEVECLAESHQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKSDVSRLPATLARIPPVAQRLQMSERSILSRLAATAGNASNAAQLMAQFPAGFQGTTLPAFTSGPAGNFANFRPQFSVPGQLSNNSGV

Target ORF information:

RefSeq Version	NM_001170005.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Mi-2, transcript variant C (Mi-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170005.2

ORF Insert Sequence:

ATGGCATCGG AGGAAGAGAA TGACGATAAT TTCCAGGAAG AAGAAGAGGC CCAGGAGGAC 
AACGCACCTG CGGCGGAGCT GTCCAACGAT TCCGACGCCC CTCTGAAGCC GAATAACGAC 
GAAGACGACG ACTACGATCC GGAGGACAGT CGCAGGAAGA AGAAGGGCAA GAAGCGCAAG 
ACCAGGAAGG GCGAAGAGAA GGGTCGCAAG AAGAAGAAGC GCAAGAAGAA CGAGAGCGAA 
GAGGACAGCG ACTTTGTCCA GCACGATGAG GAGGTGGAGT ACCCCAGCAC CTCCAAGCGC 
GGTCGCAAGC GCAAGGAGGA GAAACAGGCC GCCAAGGAGA AGGAGTCGGC ATCATCCGGA 
ATGCCATCTG TGGAAGACGT GTGCTCAGCC TTTAGCGTCT GCAATGTGGA GATCGAGTAC 
AGTGAGGAGG AGCTGCAGAG CCTAACGACC TACAAGGCTT TCATGCATCA TGTTCGTCCG 
ATTCTGCAGA AGGAAAACCC CAAGATTGCG GCACCAAAGC TAGTGATGCT GGTGGCAGCC 
AAGTGGCGTG AGTTCTGCGA GAGCAATCCG CACATCCAGC AGGAGGGTGG TGCCGCCGGC 
TCCGGTGGAT CCGCCGGACA GGCACGCAGT GTGACAGGCG ATGAGCCGGA GGAACCTCGA 
TCATCGCGTT CCTCGCGCAA CGAGAAACCG GATGACATTT ACGAGGAGGC CGTCGAGGAG 
GAGGAAGAAG AGGAGGAGGA GGAGAAGAAA CCTCGTCGTA AGCGCAGTGG ACGTGGCAAG 
AAGGGGCGTC GTCCCTCCGG CAAGGTGCCC ACTCTTAAGA TTAAGCTGCT CGGCAAGCGC 
AAACGCGATA GTTCGGATGA GGAGCAAGAT GCTAGCGGTG CATCGGAACG CGATTCGGAT 
CTTGAGTTTG AACGGATGCT TCAGAAATCG GATGACAGTG CCGATGAAAA GGAGGCTCCT 
GTCTCCTCTA AGGCGGACAA CTCAGCACCC GCTGCCCAGG ACGATGGATC AGGAGCTCCA 
GTGGTGCGCA AGAAGGCCAA GACCAAGATC GGCAACAAGT TCAAGAAGAA GAATAAACTC 
AAGAAAACGA AGAACTTCCC GGAGGGCGAA GATGGCGAGC ATGAGCACCA GGACTACTGC 
GAGGTGTGCC AGCAAGGAGG TGAGATCATC CTGTGCGACA CCTGCCCTCG GGCATATCAC 
TTGGTGTGCC TGGAGCCAGA ACTCGATGAA CCGCCAGAGG GCAAGTGGTC GTGTCCGCAC 
TGCGAGGCTG ACGGAGGTGC TGCTGAGGAA GAGGACGATG ATGAGCACCA GGAATTCTGC 
CGCGTGTGCA AGGACGGTGG CGAGTTGCTG TGCTGCGACT CATGTCCCTC CGCCTATCAC 
ACATTCTGCT TGAATCCGCC TCTGGACACT ATACCCGATG GCGATTGGCG CTGTCCTCGT 
TGCAGCTGCC CTCCGCTCAC TGGCAAGGCT GAGAAGATCA TCACCTGGCG ATGGGCACAG 
CGTAGCAATG ACGACGGTCC CTCCACCTCG AAGGGATCAA AGAACAGTAA CTCCCGGGTG 
CGCGAGTACT TTATCAAGTG GCACAACATG TCCTACTGGC ATTGCGAATG GGTGCCCGAA 
GTGCAACTGG ACGTCCATCA TCCGCTCATG ATTCGTTCGT TCCAGCGCAA GTACGACATG 
GAAGAACCGC CCAAGTTCGA GGAGTCGCTG GATGAGGCGG ACACTCGTTA CAAGCGTATT 
CAGCGACACA AGGACAAGGT TGGCATGAAG GCAAACGATG ATGCCGAAGT GCTGGAGGAG 
CGGTTCTACA AGAATGGCGT CAAGCCAGAG TGGCTCATTG TTCAGCGCGT AATCAACCAT 
CGTACGGCAA GAGATGGCAG CACCATGTAT CTGGTGAAGT GGCGCGAGTT GCCCTATGAC 
AAGTCCACCT GGGAAGAAGA GGGTGATGAC ATACAGGGCC TGCGGCAAGC CATCGATTAC 
TATCAGGATC TGCGTGCGGT TTGCACCTCG GAGACAACTC AGTCGCGCAG CAAAAAGAGC 
AAGAAGGGTC GCAAGTCCAA GCTCAAGGTC GAGGACGACG AGGATCGCCC GGTCAAGCAC 
TACACACCGC CGCCGGAAAA GCCAACTACT GATCTGAAGA AGAAGTATGA GGATCAGCCG 
GCTTTCTTGG AGGGCACTGG CATGCAATTG CATCCCTACC AGATCGAGGG CATTAACTGG 
TTGCGCTACA GCTGGGGCCA GGGCATCGAC ACCATCCTGG CCGACGAGAT GGGTCTGGGT 
AAGACCATTC AGACGGTCAC CTTCCTGTAC TCTTTGTACA AGGAGGGCCA TTGCCGCGGA 
CCCTTCCTCG TGGCCGTGCC ACTCTCGACG CTAGTGAACT GGGAGCGTGA GTTTGAGCTG 
TGGGCTCCGG ACTTTTACTG CATCACGTAT ATCGGTGACA AGGACTCGAG AGCGGTTATA 
CGGGAGAACG AGCTGAGCTT CGAGGAGGGC GCCATTCGTG GTAGCAAGGT TTCGCGTTTG 
CGGACCACCC AGTATAAATT CAACGTACTG CTGACTAGCT ACGAGCTGAT CTCCATGGAC 
GCTGCTTGCC TTGGCAGCAT TGACTGGGCC GTGCTCGTGG TGGACGAGGC CCATCGCTTG 
AAGAGCAACC AGAGCAAGTT CTTCCGTATC CTGAACAGCT ACACTATTGC CTACAAGCTT 
CTGCTCACCG GCACACCGCT GCAGAACAAT CTCGAGGAGC TGTTCCATCT GCTCAACTTC 
TTGAGCCGCG ACAAGTTCAA TGATCTGCAG GCCTTCCAGG GCGAATTCGC TGACGTTTCG 
AAGGAGGAGC AGGTTAAGCG TCTGCACGAG ATGCTTGGAC CGCACATGCT GCGTCGTTTG 
AAGACGGATG TGCTGAAGAA CATGCCGTCC AAGTCTGAGT TTATCGTGCG CGTCGAGCTG 
TCGGCCATGC AGAAAAAGTT CTACAAGTTC ATCTTGACCA AGAACTACGA GGCTTTGAAT 
TCGAAGAGCG GTGGTGGCTC TTGCTCACTG ATCAACATTA TGATGGACCT AAAGAAGTGC 
TGCAACCATC CGTATCTCTT CCCCTCGGCC GCCGAGGAGG CAACCACCGC TGCCGGTGGT 
CTCTACGAGA TTAACTCGCT GACCAAGGCT GCTGGCAAGC TGGTCCTGCT ATCCAAGATG 
CTGAAGCAGC TGAAGGCACA AAACCATCGT GTCTTGATTT TCTCCCAGAT GACCAAGATG 
TTGGACATTC TCGAGGACTT CCTCGAGGGC GAGCAGTACA AGTACGAACG TATTGACGGT 
GGAATCACAG GAACATTGCG TCAGGAAGCC ATTGATAGGT TCAATGCACC CGGAGCGCAG 
CAGTTTGTCT TCTTGCTGAG TACTCGAGCC GGAGGATTGG GTATTAATTT GGCCACGGCC 
GACACTGTCA TTATTTATGA CTCCGATTGG AATCCCCACA ACGATATTCA GGCCTTCTCC 
CGAGCCCATC GTATTGGCCA GGCTAACAAG GTGATGATCT ATCGATTTGT TACTCGAAAT 
TCTGTGGAGG AGCGCGTTAC CCAGGTGGCC AAGCGTAAGA TGATGTTGAC TCATCTTGTG 
GTCCGTCCGG GAATGGGCGG TAAAGGCGCT AACTTTACAA AGCAAGAACT GGACGATATC 
CTTCGTTTCG GTACCGAGGA TCTGTTCAAG GAGGATGACA AGGAGGAAGC TATCCACTAT 
GACGACAAGG CGGTGGCCGA GCTGCTCGAC AGAACCAACC GCGGTATCGA GGAGAAGGAG 
TCCTGGGCCA ACGAGTATCT GTCCTCGTTT AAGGTGGCTT CGTACGCCAC TAAAGAAGAG 
GAGGAGGAGG AAGAGACGGA GATTATTAAA CAGGATGCCG AGAACTCTGA TCCGGCGTAC 
TGGGTGAAGC TGCTGCGGCA TCACTATGAA CAGCACCAGG AAGATGTGGG TCGCAGCCTG 
GGCAAGGGCA AGCGCGTCCG CAAACAGGTC AACTACACGG ATGGCGGCGT TGTGGCGGCT 
GATACCACGC GGGATGATTC CAACTGGCAG GACAACGGCA GCGAGTATAA CTCCGAGTAT 
TCAGCTGGCT CCGATGAGGA TGGCGGTGAC GATGACTTCG ATGACCAGAA TGGCGCAGAG 
AGAAAGGCCA AGCGGCGATT GGAGCGTCGC GACGATCGTC CATTGCCTCC ACTGTTGGCC 
CGCGTGGGTG GAAACATCGA GGTGCTCGGT TTTAATGCCC GTCAACGCAA GAGCTTCCTC 
AATGCCATTA TGCGGTACGG AATGCCGCCG CAAGATGCCT TCAACTCGCA ATGGCTAGTC 
CGGGATTTGC GTGGTAAATC GGAGCGCAAT TTTAAGGCGT ATGTTTCTCT GTTTATGCGG 
CATCTTTGCG AGCCGGGAGC GGACAATGCG GAAACCTTTG CCGATGGTGT GCCGCGCGAA 
GGACTATCCC GCCAGCATGT GCTCACCCGG ATCGGAGTGA TGTCGCTAAT TCGAAAGAAG 
GTACAGGAAT TCGAGCATAT TAACGGATAC TACAGCATGC CGGAGCTCAT TCTCAAGCCG 
TGCGAGCCCG TACGATCAGC CCTGAAACAG GATGTGGCGG CACTTGAAGC TCCGCCTACC 
GGTGGCAATG TCGACAAGAG CGCTACTACT AGCAATAGCG TCACTCCAGC CACCAGTGCG 
GCTCCCAGTC CAGCGCCAGC CTCTGAAAAA GGTGAAGACA AAGACAAGGA CTCCGAAAAG 
GAGAAGGATA AGACCTCGGC GGAGAAGAGC GAGGTGAAGC AAGAACAAGA GGCTGAAGAG 
GACAAGAAGC CTGGGGATGT CAAGCAGGAG AACCCTGTGG AAGAAGCTGC CGGCGACACA 
AAGCCCAGCG ACGCGGAGGT CAAAACCGAG GTGGCTAAAA CAGAACCCAA GGAAGAGACC 
AAGGACCCGG AGGTAAAGGA GGAGCCCAAG ACCGAAGAGA AGGAAAAAGA AAAGGTGGAT 
GACAAGAAAC CAATACCACC GACGACAGTA ATTGACGACG ACGATGACGA TGTGATGATT 
GTCAAGGAGG ACGGTGAGCT AGAAAAGCCC AGTGCCAGTT CACCCAAGGA TCAAAAGGCA 
GTCGCCGCTG CTACGTCTGC TGCAACTGGA GCCACTGGCA AGGGAGCGGA GGACAGCTTG 
GAGGTGCTAA AGCGCAAATT TATGTTCAAC ATCGCCGACG GCGGGTTCAC CGAACTGCAC 
ACTTTGTGGC TCAACGAGGA GAAGGCTGCC GTTCCTGGGC GAGAATACGA GATCTGGCAC 
CGTCGCCACG ACTACTGGCT GTTGGCCGGA ATTGTGACCC ATGGCTATGG CCGTTGGCAG 
GATATACAAA ACGATATCCG TTTCGCGATC ATAAACGAAC CTTTCAAGAT GGACGTTGGT 
AAGGGCAACT TTCTGGAAAT CAAAAACAAG TTCCTGGCCC GGCGTTTTAA GTTGCTGGAG 
CAGGCGCTGG TCATCGAGGA GCAGCTGCGG CGAGCGGCCT ACCTTAACCT CGCCCAGGAC 
CCCAGCCACC CGGCAATGTC GCTAAACGCC CGTTTCGCAG AGGTCGAATG CCTGGCTGAA 
TCCCATCAGC ATCTGAGCAA GGAATCGCTG GCCGGTAACA AGCCCGCAAA CGCTGTTCTG 
CACAAGGTGC TCAACCAGCT CGAAGAGCTG CTCTCGGACA TGAAGAGCGA TGTGTCCCGG 
CTGCCAGCTA CACTGGCTCG CATCCCACCT GTTGCGCAAC GGCTTCAGAT GTCCGAGAGA 
TCGATTCTCT CCCGACTGGC GGCCACCGCC GGAAATGCCT CCAATGCAGC TCAATTGATG 
GCACAATTCC CGGCTGGATT CCAGGGCACA ACATTGCCAG CATTCACGAG CGGACCGGCT 
GGCAACTTTG CCAACTTTCG GCCACAGTTC TCGGTGCCCG GCCAGCTATC GAATAATTCC 
GGCGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mi-2 ( NM_140897.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649154.2 Drosophila melanogaster Mi-2, transcript variant A (Mi-2), mRNA.

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CloneID	OFa19027
Clone ID Related Accession (Same CDS sequence)	NM_001170005.2 , NM_140897.4
Accession Version	NM_140897.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Mi-2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140897.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATCGG AGGAAGAGAA TGACGATAAT TTCCAGGAAG AAGAAGAGGC CCAGGAGGAC 
AACGCACCTG CGGCGGAGCT GTCCAACGAT TCCGACGCCC CTCTGAAGCC GAATAACGAC 
GAAGACGACG ACTACGATCC GGAGGACAGT CGCAGGAAGA AGAAGGGCAA GAAGCGCAAG 
ACCAGGAAGG GCGAAGAGAA GGGTCGCAAG AAGAAGAAGC GCAAGAAGAA CGAGAGCGAA 
GAGGACAGCG ACTTTGTCCA GCACGATGAG GAGGTGGAGT ACCCCAGCAC CTCCAAGCGC 
GGTCGCAAGC GCAAGGAGGA GAAACAGGCC GCCAAGGAGA AGGAGTCGGC ATCATCCGGA 
ATGCCATCTG TGGAAGACGT GTGCTCAGCC TTTAGCGTCT GCAATGTGGA GATCGAGTAC 
AGTGAGGAGG AGCTGCAGAG CCTAACGACC TACAAGGCTT TCATGCATCA TGTTCGTCCG 
ATTCTGCAGA AGGAAAACCC CAAGATTGCG GCACCAAAGC TAGTGATGCT GGTGGCAGCC 
AAGTGGCGTG AGTTCTGCGA GAGCAATCCG CACATCCAGC AGGAGGGTGG TGCCGCCGGC 
TCCGGTGGAT CCGCCGGACA GGCACGCAGT GTGACAGGCG ATGAGCCGGA GGAACCTCGA 
TCATCGCGTT CCTCGCGCAA CGAGAAACCG GATGACATTT ACGAGGAGGC CGTCGAGGAG 
GAGGAAGAAG AGGAGGAGGA GGAGAAGAAA CCTCGTCGTA AGCGCAGTGG ACGTGGCAAG 
AAGGGGCGTC GTCCCTCCGG CAAGGTGCCC ACTCTTAAGA TTAAGCTGCT CGGCAAGCGC 
AAACGCGATA GTTCGGATGA GGAGCAAGAT GCTAGCGGTG CATCGGAACG CGATTCGGAT 
CTTGAGTTTG AACGGATGCT TCAGAAATCG GATGACAGTG CCGATGAAAA GGAGGCTCCT 
GTCTCCTCTA AGGCGGACAA CTCAGCACCC GCTGCCCAGG ACGATGGATC AGGAGCTCCA 
GTGGTGCGCA AGAAGGCCAA GACCAAGATC GGCAACAAGT TCAAGAAGAA GAATAAACTC 
AAGAAAACGA AGAACTTCCC GGAGGGCGAA GATGGCGAGC ATGAGCACCA GGACTACTGC 
GAGGTGTGCC AGCAAGGAGG TGAGATCATC CTGTGCGACA CCTGCCCTCG GGCATATCAC 
TTGGTGTGCC TGGAGCCAGA ACTCGATGAA CCGCCAGAGG GCAAGTGGTC GTGTCCGCAC 
TGCGAGGCTG ACGGAGGTGC TGCTGAGGAA GAGGACGATG ATGAGCACCA GGAATTCTGC 
CGCGTGTGCA AGGACGGTGG CGAGTTGCTG TGCTGCGACT CATGTCCCTC CGCCTATCAC 
ACATTCTGCT TGAATCCGCC TCTGGACACT ATACCCGATG GCGATTGGCG CTGTCCTCGT 
TGCAGCTGCC CTCCGCTCAC TGGCAAGGCT GAGAAGATCA TCACCTGGCG ATGGGCACAG 
CGTAGCAATG ACGACGGTCC CTCCACCTCG AAGGGATCAA AGAACAGTAA CTCCCGGGTG 
CGCGAGTACT TTATCAAGTG GCACAACATG TCCTACTGGC ATTGCGAATG GGTGCCCGAA 
GTGCAACTGG ACGTCCATCA TCCGCTCATG ATTCGTTCGT TCCAGCGCAA GTACGACATG 
GAAGAACCGC CCAAGTTCGA GGAGTCGCTG GATGAGGCGG ACACTCGTTA CAAGCGTATT 
CAGCGACACA AGGACAAGGT TGGCATGAAG GCAAACGATG ATGCCGAAGT GCTGGAGGAG 
CGGTTCTACA AGAATGGCGT CAAGCCAGAG TGGCTCATTG TTCAGCGCGT AATCAACCAT 
CGTACGGCAA GAGATGGCAG CACCATGTAT CTGGTGAAGT GGCGCGAGTT GCCCTATGAC 
AAGTCCACCT GGGAAGAAGA GGGTGATGAC ATACAGGGCC TGCGGCAAGC CATCGATTAC 
TATCAGGATC TGCGTGCGGT TTGCACCTCG GAGACAACTC AGTCGCGCAG CAAAAAGAGC 
AAGAAGGGTC GCAAGTCCAA GCTCAAGGTC GAGGACGACG AGGATCGCCC GGTCAAGCAC 
TACACACCGC CGCCGGAAAA GCCAACTACT GATCTGAAGA AGAAGTATGA GGATCAGCCG 
GCTTTCTTGG AGGGCACTGG CATGCAATTG CATCCCTACC AGATCGAGGG CATTAACTGG 
TTGCGCTACA GCTGGGGCCA GGGCATCGAC ACCATCCTGG CCGACGAGAT GGGTCTGGGT 
AAGACCATTC AGACGGTCAC CTTCCTGTAC TCTTTGTACA AGGAGGGCCA TTGCCGCGGA 
CCCTTCCTCG TGGCCGTGCC ACTCTCGACG CTAGTGAACT GGGAGCGTGA GTTTGAGCTG 
TGGGCTCCGG ACTTTTACTG CATCACGTAT ATCGGTGACA AGGACTCGAG AGCGGTTATA 
CGGGAGAACG AGCTGAGCTT CGAGGAGGGC GCCATTCGTG GTAGCAAGGT TTCGCGTTTG 
CGGACCACCC AGTATAAATT CAACGTACTG CTGACTAGCT ACGAGCTGAT CTCCATGGAC 
GCTGCTTGCC TTGGCAGCAT TGACTGGGCC GTGCTCGTGG TGGACGAGGC CCATCGCTTG 
AAGAGCAACC AGAGCAAGTT CTTCCGTATC CTGAACAGCT ACACTATTGC CTACAAGCTT 
CTGCTCACCG GCACACCGCT GCAGAACAAT CTCGAGGAGC TGTTCCATCT GCTCAACTTC 
TTGAGCCGCG ACAAGTTCAA TGATCTGCAG GCCTTCCAGG GCGAATTCGC TGACGTTTCG 
AAGGAGGAGC AGGTTAAGCG TCTGCACGAG ATGCTTGGAC CGCACATGCT GCGTCGTTTG 
AAGACGGATG TGCTGAAGAA CATGCCGTCC AAGTCTGAGT TTATCGTGCG CGTCGAGCTG 
TCGGCCATGC AGAAAAAGTT CTACAAGTTC ATCTTGACCA AGAACTACGA GGCTTTGAAT 
TCGAAGAGCG GTGGTGGCTC TTGCTCACTG ATCAACATTA TGATGGACCT AAAGAAGTGC 
TGCAACCATC CGTATCTCTT CCCCTCGGCC GCCGAGGAGG CAACCACCGC TGCCGGTGGT 
CTCTACGAGA TTAACTCGCT GACCAAGGCT GCTGGCAAGC TGGTCCTGCT ATCCAAGATG 
CTGAAGCAGC TGAAGGCACA AAACCATCGT GTCTTGATTT TCTCCCAGAT GACCAAGATG 
TTGGACATTC TCGAGGACTT CCTCGAGGGC GAGCAGTACA AGTACGAACG TATTGACGGT 
GGAATCACAG GAACATTGCG TCAGGAAGCC ATTGATAGGT TCAATGCACC CGGAGCGCAG 
CAGTTTGTCT TCTTGCTGAG TACTCGAGCC GGAGGATTGG GTATTAATTT GGCCACGGCC 
GACACTGTCA TTATTTATGA CTCCGATTGG AATCCCCACA ACGATATTCA GGCCTTCTCC 
CGAGCCCATC GTATTGGCCA GGCTAACAAG GTGATGATCT ATCGATTTGT TACTCGAAAT 
TCTGTGGAGG AGCGCGTTAC CCAGGTGGCC AAGCGTAAGA TGATGTTGAC TCATCTTGTG 
GTCCGTCCGG GAATGGGCGG TAAAGGCGCT AACTTTACAA AGCAAGAACT GGACGATATC 
CTTCGTTTCG GTACCGAGGA TCTGTTCAAG GAGGATGACA AGGAGGAAGC TATCCACTAT 
GACGACAAGG CGGTGGCCGA GCTGCTCGAC AGAACCAACC GCGGTATCGA GGAGAAGGAG 
TCCTGGGCCA ACGAGTATCT GTCCTCGTTT AAGGTGGCTT CGTACGCCAC TAAAGAAGAG 
GAGGAGGAGG AAGAGACGGA GATTATTAAA CAGGATGCCG AGAACTCTGA TCCGGCGTAC 
TGGGTGAAGC TGCTGCGGCA TCACTATGAA CAGCACCAGG AAGATGTGGG TCGCAGCCTG 
GGCAAGGGCA AGCGCGTCCG CAAACAGGTC AACTACACGG ATGGCGGCGT TGTGGCGGCT 
GATACCACGC GGGATGATTC CAACTGGCAG GACAACGGCA GCGAGTATAA CTCCGAGTAT 
TCAGCTGGCT CCGATGAGGA TGGCGGTGAC GATGACTTCG ATGACCAGAA TGGCGCAGAG 
AGAAAGGCCA AGCGGCGATT GGAGCGTCGC GACGATCGTC CATTGCCTCC ACTGTTGGCC 
CGCGTGGGTG GAAACATCGA GGTGCTCGGT TTTAATGCCC GTCAACGCAA GAGCTTCCTC 
AATGCCATTA TGCGGTACGG AATGCCGCCG CAAGATGCCT TCAACTCGCA ATGGCTAGTC 
CGGGATTTGC GTGGTAAATC GGAGCGCAAT TTTAAGGCGT ATGTTTCTCT GTTTATGCGG 
CATCTTTGCG AGCCGGGAGC GGACAATGCG GAAACCTTTG CCGATGGTGT GCCGCGCGAA 
GGACTATCCC GCCAGCATGT GCTCACCCGG ATCGGAGTGA TGTCGCTAAT TCGAAAGAAG 
GTACAGGAAT TCGAGCATAT TAACGGATAC TACAGCATGC CGGAGCTCAT TCTCAAGCCG 
TGCGAGCCCG TACGATCAGC CCTGAAACAG GATGTGGCGG CACTTGAAGC TCCGCCTACC 
GGTGGCAATG TCGACAAGAG CGCTACTACT AGCAATAGCG TCACTCCAGC CACCAGTGCG 
GCTCCCAGTC CAGCGCCAGC CTCTGAAAAA GGTGAAGACA AAGACAAGGA CTCCGAAAAG 
GAGAAGGATA AGACCTCGGC GGAGAAGAGC GAGGTGAAGC AAGAACAAGA GGCTGAAGAG 
GACAAGAAGC CTGGGGATGT CAAGCAGGAG AACCCTGTGG AAGAAGCTGC CGGCGACACA 
AAGCCCAGCG ACGCGGAGGT CAAAACCGAG GTGGCTAAAA CAGAACCCAA GGAAGAGACC 
AAGGACCCGG AGGTAAAGGA GGAGCCCAAG ACCGAAGAGA AGGAAAAAGA AAAGGTGGAT 
GACAAGAAAC CAATACCACC GACGACAGTA ATTGACGACG ACGATGACGA TGTGATGATT 
GTCAAGGAGG ACGGTGAGCT AGAAAAGCCC AGTGCCAGTT CACCCAAGGA TCAAAAGGCA 
GTCGCCGCTG CTACGTCTGC TGCAACTGGA GCCACTGGCA AGGGAGCGGA GGACAGCTTG 
GAGGTGCTAA AGCGCAAATT TATGTTCAAC ATCGCCGACG GCGGGTTCAC CGAACTGCAC 
ACTTTGTGGC TCAACGAGGA GAAGGCTGCC GTTCCTGGGC GAGAATACGA GATCTGGCAC 
CGTCGCCACG ACTACTGGCT GTTGGCCGGA ATTGTGACCC ATGGCTATGG CCGTTGGCAG 
GATATACAAA ACGATATCCG TTTCGCGATC ATAAACGAAC CTTTCAAGAT GGACGTTGGT 
AAGGGCAACT TTCTGGAAAT CAAAAACAAG TTCCTGGCCC GGCGTTTTAA GTTGCTGGAG 
CAGGCGCTGG TCATCGAGGA GCAGCTGCGG CGAGCGGCCT ACCTTAACCT CGCCCAGGAC 
CCCAGCCACC CGGCAATGTC GCTAAACGCC CGTTTCGCAG AGGTCGAATG CCTGGCTGAA 
TCCCATCAGC ATCTGAGCAA GGAATCGCTG GCCGGTAACA AGCCCGCAAA CGCTGTTCTG 
CACAAGGTGC TCAACCAGCT CGAAGAGCTG CTCTCGGACA TGAAGAGCGA TGTGTCCCGG 
CTGCCAGCTA CACTGGCTCG CATCCCACCT GTTGCGCAAC GGCTTCAGAT GTCCGAGAGA 
TCGATTCTCT CCCGACTGGC GGCCACCGCC GGAAATGCCT CCAATGCAGC TCAATTGATG 
GCACAATTCC CGGCTGGATT CCAGGGCACA ACATTGCCAG CATTCACGAG CGGACCGGCT 
GGCAACTTTG CCAACTTTCG GCCACAGTTC TCGGTGCCCG GCCAGCTATC GAATAATTCC 
GGCGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649154.2
CDS	137..6085
Translation	MASEEENDDNFQEEEEAQEDNAPAAELSNDSDAPLKPNNDEDDDYDPEDSRRKKKGKKRKTRKGEEKGRKKKKRKKNESEEDSDFVQHDEEVEYPSTSKRGRKRKEEKQAAKEKESASSGMPSVEDVCSAFSVCNVEIEYSEEELQSLTTYKAFMHHVRPILQKENPKIAAPKLVMLVAAKWREFCESNPHIQQEGGAAGSGGSAGQARSVTGDEPEEPRSSRSSRNEKPDDIYEEAVEEEEEEEEEEKKPRRKRSGRGKKGRRPSGKVPTLKIKLLGKRKRDSSDEEQDASGASERDSDLEFERMLQKSDDSADEKEAPVSSKADNSAPAAQDDGSGAPVVRKKAKTKIGNKFKKKNKLKKTKNFPEGEDGEHEHQDYCEVCQQGGEIILCDTCPRAYHLVCLEPELDEPPEGKWSCPHCEADGGAAEEEDDDEHQEFCRVCKDGGELLCCDSCPSAYHTFCLNPPLDTIPDGDWRCPRCSCPPLTGKAEKIITWRWAQRSNDDGPSTSKGSKNSNSRVREYFIKWHNMSYWHCEWVPEVQLDVHHPLMIRSFQRKYDMEEPPKFEESLDEADTRYKRIQRHKDKVGMKANDDAEVLEERFYKNGVKPEWLIVQRVINHRTARDGSTMYLVKWRELPYDKSTWEEEGDDIQGLRQAIDYYQDLRAVCTSETTQSRSKKSKKGRKSKLKVEDDEDRPVKHYTPPPEKPTTDLKKKYEDQPAFLEGTGMQLHPYQIEGINWLRYSWGQGIDTILADEMGLGKTIQTVTFLYSLYKEGHCRGPFLVAVPLSTLVNWEREFELWAPDFYCITYIGDKDSRAVIRENELSFEEGAIRGSKVSRLRTTQYKFNVLLTSYELISMDAACLGSIDWAVLVVDEAHRLKSNQSKFFRILNSYTIAYKLLLTGTPLQNNLEELFHLLNFLSRDKFNDLQAFQGEFADVSKEEQVKRLHEMLGPHMLRRLKTDVLKNMPSKSEFIVRVELSAMQKKFYKFILTKNYEALNSKSGGGSCSLINIMMDLKKCCNHPYLFPSAAEEATTAAGGLYEINSLTKAAGKLVLLSKMLKQLKAQNHRVLIFSQMTKMLDILEDFLEGEQYKYERIDGGITGTLRQEAIDRFNAPGAQQFVFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQANKVMIYRFVTRNSVEERVTQVAKRKMMLTHLVVRPGMGGKGANFTKQELDDILRFGTEDLFKEDDKEEAIHYDDKAVAELLDRTNRGIEEKESWANEYLSSFKVASYATKEEEEEEETEIIKQDAENSDPAYWVKLLRHHYEQHQEDVGRSLGKGKRVRKQVNYTDGGVVAADTTRDDSNWQDNGSEYNSEYSAGSDEDGGDDDFDDQNGAERKAKRRLERRDDRPLPPLLARVGGNIEVLGFNARQRKSFLNAIMRYGMPPQDAFNSQWLVRDLRGKSERNFKAYVSLFMRHLCEPGADNAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHINGYYSMPELILKPCEPVRSALKQDVAALEAPPTGGNVDKSATTSNSVTPATSAAPSPAPASEKGEDKDKDSEKEKDKTSAEKSEVKQEQEAEEDKKPGDVKQENPVEEAAGDTKPSDAEVKTEVAKTEPKEETKDPEVKEEPKTEEKEKEKVDDKKPIPPTTVIDDDDDDVMIVKEDGELEKPSASSPKDQKAVAAATSAATGATGKGAEDSLEVLKRKFMFNIADGGFTELHTLWLNEEKAAVPGREYEIWHRRHDYWLLAGIVTHGYGRWQDIQNDIRFAIINEPFKMDVGKGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNLAQDPSHPAMSLNARFAEVECLAESHQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKSDVSRLPATLARIPPVAQRLQMSERSILSRLAATAGNASNAAQLMAQFPAGFQGTTLPAFTSGPAGNFANFRPQFSVPGQLSNNSGV

Target ORF information:

RefSeq Version	NM_140897.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Mi-2, transcript variant A (Mi-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140897.4

ORF Insert Sequence:

ATGGCATCGG AGGAAGAGAA TGACGATAAT TTCCAGGAAG AAGAAGAGGC CCAGGAGGAC 
AACGCACCTG CGGCGGAGCT GTCCAACGAT TCCGACGCCC CTCTGAAGCC GAATAACGAC 
GAAGACGACG ACTACGATCC GGAGGACAGT CGCAGGAAGA AGAAGGGCAA GAAGCGCAAG 
ACCAGGAAGG GCGAAGAGAA GGGTCGCAAG AAGAAGAAGC GCAAGAAGAA CGAGAGCGAA 
GAGGACAGCG ACTTTGTCCA GCACGATGAG GAGGTGGAGT ACCCCAGCAC CTCCAAGCGC 
GGTCGCAAGC GCAAGGAGGA GAAACAGGCC GCCAAGGAGA AGGAGTCGGC ATCATCCGGA 
ATGCCATCTG TGGAAGACGT GTGCTCAGCC TTTAGCGTCT GCAATGTGGA GATCGAGTAC 
AGTGAGGAGG AGCTGCAGAG CCTAACGACC TACAAGGCTT TCATGCATCA TGTTCGTCCG 
ATTCTGCAGA AGGAAAACCC CAAGATTGCG GCACCAAAGC TAGTGATGCT GGTGGCAGCC 
AAGTGGCGTG AGTTCTGCGA GAGCAATCCG CACATCCAGC AGGAGGGTGG TGCCGCCGGC 
TCCGGTGGAT CCGCCGGACA GGCACGCAGT GTGACAGGCG ATGAGCCGGA GGAACCTCGA 
TCATCGCGTT CCTCGCGCAA CGAGAAACCG GATGACATTT ACGAGGAGGC CGTCGAGGAG 
GAGGAAGAAG AGGAGGAGGA GGAGAAGAAA CCTCGTCGTA AGCGCAGTGG ACGTGGCAAG 
AAGGGGCGTC GTCCCTCCGG CAAGGTGCCC ACTCTTAAGA TTAAGCTGCT CGGCAAGCGC 
AAACGCGATA GTTCGGATGA GGAGCAAGAT GCTAGCGGTG CATCGGAACG CGATTCGGAT 
CTTGAGTTTG AACGGATGCT TCAGAAATCG GATGACAGTG CCGATGAAAA GGAGGCTCCT 
GTCTCCTCTA AGGCGGACAA CTCAGCACCC GCTGCCCAGG ACGATGGATC AGGAGCTCCA 
GTGGTGCGCA AGAAGGCCAA GACCAAGATC GGCAACAAGT TCAAGAAGAA GAATAAACTC 
AAGAAAACGA AGAACTTCCC GGAGGGCGAA GATGGCGAGC ATGAGCACCA GGACTACTGC 
GAGGTGTGCC AGCAAGGAGG TGAGATCATC CTGTGCGACA CCTGCCCTCG GGCATATCAC 
TTGGTGTGCC TGGAGCCAGA ACTCGATGAA CCGCCAGAGG GCAAGTGGTC GTGTCCGCAC 
TGCGAGGCTG ACGGAGGTGC TGCTGAGGAA GAGGACGATG ATGAGCACCA GGAATTCTGC 
CGCGTGTGCA AGGACGGTGG CGAGTTGCTG TGCTGCGACT CATGTCCCTC CGCCTATCAC 
ACATTCTGCT TGAATCCGCC TCTGGACACT ATACCCGATG GCGATTGGCG CTGTCCTCGT 
TGCAGCTGCC CTCCGCTCAC TGGCAAGGCT GAGAAGATCA TCACCTGGCG ATGGGCACAG 
CGTAGCAATG ACGACGGTCC CTCCACCTCG AAGGGATCAA AGAACAGTAA CTCCCGGGTG 
CGCGAGTACT TTATCAAGTG GCACAACATG TCCTACTGGC ATTGCGAATG GGTGCCCGAA 
GTGCAACTGG ACGTCCATCA TCCGCTCATG ATTCGTTCGT TCCAGCGCAA GTACGACATG 
GAAGAACCGC CCAAGTTCGA GGAGTCGCTG GATGAGGCGG ACACTCGTTA CAAGCGTATT 
CAGCGACACA AGGACAAGGT TGGCATGAAG GCAAACGATG ATGCCGAAGT GCTGGAGGAG 
CGGTTCTACA AGAATGGCGT CAAGCCAGAG TGGCTCATTG TTCAGCGCGT AATCAACCAT 
CGTACGGCAA GAGATGGCAG CACCATGTAT CTGGTGAAGT GGCGCGAGTT GCCCTATGAC 
AAGTCCACCT GGGAAGAAGA GGGTGATGAC ATACAGGGCC TGCGGCAAGC CATCGATTAC 
TATCAGGATC TGCGTGCGGT TTGCACCTCG GAGACAACTC AGTCGCGCAG CAAAAAGAGC 
AAGAAGGGTC GCAAGTCCAA GCTCAAGGTC GAGGACGACG AGGATCGCCC GGTCAAGCAC 
TACACACCGC CGCCGGAAAA GCCAACTACT GATCTGAAGA AGAAGTATGA GGATCAGCCG 
GCTTTCTTGG AGGGCACTGG CATGCAATTG CATCCCTACC AGATCGAGGG CATTAACTGG 
TTGCGCTACA GCTGGGGCCA GGGCATCGAC ACCATCCTGG CCGACGAGAT GGGTCTGGGT 
AAGACCATTC AGACGGTCAC CTTCCTGTAC TCTTTGTACA AGGAGGGCCA TTGCCGCGGA 
CCCTTCCTCG TGGCCGTGCC ACTCTCGACG CTAGTGAACT GGGAGCGTGA GTTTGAGCTG 
TGGGCTCCGG ACTTTTACTG CATCACGTAT ATCGGTGACA AGGACTCGAG AGCGGTTATA 
CGGGAGAACG AGCTGAGCTT CGAGGAGGGC GCCATTCGTG GTAGCAAGGT TTCGCGTTTG 
CGGACCACCC AGTATAAATT CAACGTACTG CTGACTAGCT ACGAGCTGAT CTCCATGGAC 
GCTGCTTGCC TTGGCAGCAT TGACTGGGCC GTGCTCGTGG TGGACGAGGC CCATCGCTTG 
AAGAGCAACC AGAGCAAGTT CTTCCGTATC CTGAACAGCT ACACTATTGC CTACAAGCTT 
CTGCTCACCG GCACACCGCT GCAGAACAAT CTCGAGGAGC TGTTCCATCT GCTCAACTTC 
TTGAGCCGCG ACAAGTTCAA TGATCTGCAG GCCTTCCAGG GCGAATTCGC TGACGTTTCG 
AAGGAGGAGC AGGTTAAGCG TCTGCACGAG ATGCTTGGAC CGCACATGCT GCGTCGTTTG 
AAGACGGATG TGCTGAAGAA CATGCCGTCC AAGTCTGAGT TTATCGTGCG CGTCGAGCTG 
TCGGCCATGC AGAAAAAGTT CTACAAGTTC ATCTTGACCA AGAACTACGA GGCTTTGAAT 
TCGAAGAGCG GTGGTGGCTC TTGCTCACTG ATCAACATTA TGATGGACCT AAAGAAGTGC 
TGCAACCATC CGTATCTCTT CCCCTCGGCC GCCGAGGAGG CAACCACCGC TGCCGGTGGT 
CTCTACGAGA TTAACTCGCT GACCAAGGCT GCTGGCAAGC TGGTCCTGCT ATCCAAGATG 
CTGAAGCAGC TGAAGGCACA AAACCATCGT GTCTTGATTT TCTCCCAGAT GACCAAGATG 
TTGGACATTC TCGAGGACTT CCTCGAGGGC GAGCAGTACA AGTACGAACG TATTGACGGT 
GGAATCACAG GAACATTGCG TCAGGAAGCC ATTGATAGGT TCAATGCACC CGGAGCGCAG 
CAGTTTGTCT TCTTGCTGAG TACTCGAGCC GGAGGATTGG GTATTAATTT GGCCACGGCC 
GACACTGTCA TTATTTATGA CTCCGATTGG AATCCCCACA ACGATATTCA GGCCTTCTCC 
CGAGCCCATC GTATTGGCCA GGCTAACAAG GTGATGATCT ATCGATTTGT TACTCGAAAT 
TCTGTGGAGG AGCGCGTTAC CCAGGTGGCC AAGCGTAAGA TGATGTTGAC TCATCTTGTG 
GTCCGTCCGG GAATGGGCGG TAAAGGCGCT AACTTTACAA AGCAAGAACT GGACGATATC 
CTTCGTTTCG GTACCGAGGA TCTGTTCAAG GAGGATGACA AGGAGGAAGC TATCCACTAT 
GACGACAAGG CGGTGGCCGA GCTGCTCGAC AGAACCAACC GCGGTATCGA GGAGAAGGAG 
TCCTGGGCCA ACGAGTATCT GTCCTCGTTT AAGGTGGCTT CGTACGCCAC TAAAGAAGAG 
GAGGAGGAGG AAGAGACGGA GATTATTAAA CAGGATGCCG AGAACTCTGA TCCGGCGTAC 
TGGGTGAAGC TGCTGCGGCA TCACTATGAA CAGCACCAGG AAGATGTGGG TCGCAGCCTG 
GGCAAGGGCA AGCGCGTCCG CAAACAGGTC AACTACACGG ATGGCGGCGT TGTGGCGGCT 
GATACCACGC GGGATGATTC CAACTGGCAG GACAACGGCA GCGAGTATAA CTCCGAGTAT 
TCAGCTGGCT CCGATGAGGA TGGCGGTGAC GATGACTTCG ATGACCAGAA TGGCGCAGAG 
AGAAAGGCCA AGCGGCGATT GGAGCGTCGC GACGATCGTC CATTGCCTCC ACTGTTGGCC 
CGCGTGGGTG GAAACATCGA GGTGCTCGGT TTTAATGCCC GTCAACGCAA GAGCTTCCTC 
AATGCCATTA TGCGGTACGG AATGCCGCCG CAAGATGCCT TCAACTCGCA ATGGCTAGTC 
CGGGATTTGC GTGGTAAATC GGAGCGCAAT TTTAAGGCGT ATGTTTCTCT GTTTATGCGG 
CATCTTTGCG AGCCGGGAGC GGACAATGCG GAAACCTTTG CCGATGGTGT GCCGCGCGAA 
GGACTATCCC GCCAGCATGT GCTCACCCGG ATCGGAGTGA TGTCGCTAAT TCGAAAGAAG 
GTACAGGAAT TCGAGCATAT TAACGGATAC TACAGCATGC CGGAGCTCAT TCTCAAGCCG 
TGCGAGCCCG TACGATCAGC CCTGAAACAG GATGTGGCGG CACTTGAAGC TCCGCCTACC 
GGTGGCAATG TCGACAAGAG CGCTACTACT AGCAATAGCG TCACTCCAGC CACCAGTGCG 
GCTCCCAGTC CAGCGCCAGC CTCTGAAAAA GGTGAAGACA AAGACAAGGA CTCCGAAAAG 
GAGAAGGATA AGACCTCGGC GGAGAAGAGC GAGGTGAAGC AAGAACAAGA GGCTGAAGAG 
GACAAGAAGC CTGGGGATGT CAAGCAGGAG AACCCTGTGG AAGAAGCTGC CGGCGACACA 
AAGCCCAGCG ACGCGGAGGT CAAAACCGAG GTGGCTAAAA CAGAACCCAA GGAAGAGACC 
AAGGACCCGG AGGTAAAGGA GGAGCCCAAG ACCGAAGAGA AGGAAAAAGA AAAGGTGGAT 
GACAAGAAAC CAATACCACC GACGACAGTA ATTGACGACG ACGATGACGA TGTGATGATT 
GTCAAGGAGG ACGGTGAGCT AGAAAAGCCC AGTGCCAGTT CACCCAAGGA TCAAAAGGCA 
GTCGCCGCTG CTACGTCTGC TGCAACTGGA GCCACTGGCA AGGGAGCGGA GGACAGCTTG 
GAGGTGCTAA AGCGCAAATT TATGTTCAAC ATCGCCGACG GCGGGTTCAC CGAACTGCAC 
ACTTTGTGGC TCAACGAGGA GAAGGCTGCC GTTCCTGGGC GAGAATACGA GATCTGGCAC 
CGTCGCCACG ACTACTGGCT GTTGGCCGGA ATTGTGACCC ATGGCTATGG CCGTTGGCAG 
GATATACAAA ACGATATCCG TTTCGCGATC ATAAACGAAC CTTTCAAGAT GGACGTTGGT 
AAGGGCAACT TTCTGGAAAT CAAAAACAAG TTCCTGGCCC GGCGTTTTAA GTTGCTGGAG 
CAGGCGCTGG TCATCGAGGA GCAGCTGCGG CGAGCGGCCT ACCTTAACCT CGCCCAGGAC 
CCCAGCCACC CGGCAATGTC GCTAAACGCC CGTTTCGCAG AGGTCGAATG CCTGGCTGAA 
TCCCATCAGC ATCTGAGCAA GGAATCGCTG GCCGGTAACA AGCCCGCAAA CGCTGTTCTG 
CACAAGGTGC TCAACCAGCT CGAAGAGCTG CTCTCGGACA TGAAGAGCGA TGTGTCCCGG 
CTGCCAGCTA CACTGGCTCG CATCCCACCT GTTGCGCAAC GGCTTCAGAT GTCCGAGAGA 
TCGATTCTCT CCCGACTGGC GGCCACCGCC GGAAATGCCT CCAATGCAGC TCAATTGATG 
GCACAATTCC CGGCTGGATT CCAGGGCACA ACATTGCCAG CATTCACGAG CGGACCGGCT 
GGCAACTTTG CCAACTTTCG GCCACAGTTC TCGGTGCCCG GCCAGCTATC GAATAATTCC 
GGCGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mi-2 ( NM_001014591.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014591.1 Drosophila melanogaster Mi-2, transcript variant B (Mi-2), mRNA.

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CloneID	OFa19028
Clone ID Related Accession (Same CDS sequence)	NM_001014591.3
Accession Version	NM_001014591.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Mi-2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001014591.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATCGG AGGAAGAGAA TGACGATAAT TTCCAGGAAG AAGAAGAGGC CCAGGAGGAC 
AACGCACCTG CGGCGGAGCT GTCCAACGAT TCCGACGCCC CTCTGAAGCC GAATCAGAAC 
GACGAAGACG ACGACTACGA TCCGGAGGAC AGTCGCAGGA AGAAGAAGGG CAAGAAGCGC 
AAGACCAGGA AGGGCGAAGA GAAGGGTCGC AAGAAGAAGA AGCGCAAGAA GAACGAGAGC 
GAAGAGGACA GCGACTTTGT CCAGCACGAT GAGGAGGTGG AGTACCCCAG CACCTCCAAG 
CGCGGTCGCA AGCGCAAGGA GGAGAAACAG GCCGCCAAGG AGAAGGAGTC GGCATCATCC 
GGAATGCCAT CTGTGGAAGA CGTGTGCTCA GCCTTTAGCG TCTGCAATGT GGAGATCGAG 
TACAGTGAGG AGGAGCTGCA GAGCCTAACG ACCTACAAGG CTTTCATGCA TCATGTTCGT 
CCGATTCTGC AGAAGGAAAA CCCCAAGATT GCGGCACCAA AGCTAGTGAT GCTGGTGGCA 
GCCAAGTGGC GTGAGTTCTG CGAGAGCAAT CCGCACATCC AGCAGGAGGG TGGTGCCGCC 
GGCTCCGGTG GATCCGCCGG ACAGGCACGC AGTGTGACAG GCGATGAGCC GGAGGAACCT 
CGATCATCGC GTTCCTCGCG CAACGAGAAA CCGGATGACA TTTACGAGGA GGCCGTCGAG 
GAGGAGGAAG AAGAGGAGGA GGAGGAGAAG AAACCTCGTC GTAAGCGCAG TGGACGTGGC 
AAGAAGGGGC GTCGTCCCTC CGGCAAGGTG CCCACTCTTA AGATTAAGCT GCTCGGCAAG 
CGCAAACGCG ATAGTTCGGA TGAGGAGCAA GATGCTAGCG GTGCATCGGA ACGCGATTCG 
GATCTTGAGT TTGAACGGAT GCTTCAGAAA TCGGATGACA GTGCCGATGA AAAGGAGGCT 
CCTGTCTCCT CTAAGGCGGA CAACTCAGCA CCCGCTGCCC AGGACGATGG ATCAGGAGCT 
CCAGTGGTGC GCAAGAAGGC CAAGACCAAG ATCGGCAACA AGTTCAAGAA GAAGAATAAA 
CTCAAGAAAA CGAAGAACTT CCCGGAGGGC GAAGATGGCG AGCATGAGCA CCAGGACTAC 
TGCGAGGTGT GCCAGCAAGG AGGTGAGATC ATCCTGTGCG ACACCTGCCC TCGGGCATAT 
CACTTGGTGT GCCTGGAGCC AGAACTCGAT GAACCGCCAG AGGGCAAGTG GTCGTGTCCG 
CACTGCGAGG CTGACGGAGG TGCTGCTGAG GAAGAGGACG ATGATGAGCA CCAGGAATTC 
TGCCGCGTGT GCAAGGACGG TGGCGAGTTG CTGTGCTGCG ACTCATGTCC CTCCGCCTAT 
CACACATTCT GCTTGAATCC GCCTCTGGAC ACTATACCCG ATGGCGATTG GCGCTGTCCT 
CGTTGCAGCT GCCCTCCGCT CACTGGCAAG GCTGAGAAGA TCATCACCTG GCGATGGGCA 
CAGCGTAGCA ATGACGACGG TCCCTCCACC TCGAAGGGAT CAAAGAACAG TAACTCCCGG 
GTGCGCGAGT ACTTTATCAA GTGGCACAAC ATGTCCTACT GGCATTGCGA ATGGGTGCCC 
GAAGTGCAAC TGGACGTCCA TCATCCGCTC ATGATTCGTT CGTTCCAGCG CAAGTACGAC 
ATGGAAGAAC CGCCCAAGTT CGAGGAGTCG CTGGATGAGG CGGACACTCG TTACAAGCGT 
ATTCAGCGAC ACAAGGACAA GGTTGGCATG AAGGCAAACG ATGATGCCGA AGTGCTGGAG 
GAGCGGTTCT ACAAGAATGG CGTCAAGCCA GAGTGGCTCA TTGTTCAGCG CGTAATCAAC 
CATCGTACGG CAAGAGATGG CAGCACCATG TATCTGGTGA AGTGGCGCGA GTTGCCCTAT 
GACAAGTCCA CCTGGGAAGA AGAGGGTGAT GACATACAGG GCCTGCGGCA AGCCATCGAT 
TACTATCAGG ATCTGCGTGC GGTTTGCACC TCGGAGACAA CTCAGTCGCG CAGCAAAAAG 
AGCAAGAAGG GTCGCAAGTC CAAGCTCAAG GTCGAGGACG ACGAGGATCG CCCGGTCAAG 
CACTACACAC CGCCGCCGGA AAAGCCAACT ACTGATCTGA AGAAGAAGTA TGAGGATCAG 
CCGGCTTTCT TGGAGGGCAC TGGCATGCAA TTGCATCCCT ACCAGATCGA GGGCATTAAC 
TGGTTGCGCT ACAGCTGGGG CCAGGGCATC GACACCATCC TGGCCGACGA GATGGGTCTG 
GGTAAGACCA TTCAGACGGT CACCTTCCTG TACTCTTTGT ACAAGGAGGG CCATTGCCGC 
GGACCCTTCC TCGTGGCCGT GCCACTCTCG ACGCTAGTGA ACTGGGAGCG TGAGTTTGAG 
CTGTGGGCTC CGGACTTTTA CTGCATCACG TATATCGGTG ACAAGGACTC GAGAGCGGTT 
ATACGGGAGA ACGAGCTGAG CTTCGAGGAG GGCGCCATTC GTGGTAGCAA GGTTTCGCGT 
TTGCGGACCA CCCAGTATAA ATTCAACGTA CTGCTGACTA GCTACGAGCT GATCTCCATG 
GACGCTGCTT GCCTTGGCAG CATTGACTGG GCCGTGCTCG TGGTGGACGA GGCCCATCGC 
TTGAAGAGCA ACCAGAGCAA GTTCTTCCGT ATCCTGAACA GCTACACTAT TGCCTACAAG 
CTTCTGCTCA CCGGCACACC GCTGCAGAAC AATCTCGAGG AGCTGTTCCA TCTGCTCAAC 
TTCTTGAGCC GCGACAAGTT CAATGATCTG CAGGCCTTCC AGGGCGAATT CGCTGACGTT 
TCGAAGGAGG AGCAGGTTAA GCGTCTGCAC GAGATGCTTG GACCGCACAT GCTGCGTCGT 
TTGAAGACGG ATGTGCTGAA GAACATGCCG TCCAAGTCTG AGTTTATCGT GCGCGTCGAG 
CTGTCGGCCA TGCAGAAAAA GTTCTACAAG TTCATCTTGA CCAAGAACTA CGAGGCTTTG 
AATTCGAAGA GCGGTGGTGG CTCTTGCTCA CTGATCAACA TTATGATGGA CCTAAAGAAG 
TGCTGCAACC ATCCGTATCT CTTCCCCTCG GCCGCCGAGG AGGCAACCAC CGCTGCCGGT 
GGTCTCTACG AGATTAACTC GCTGACCAAG GCTGCTGGCA AGCTGGTCCT GCTATCCAAG 
ATGCTGAAGC AGCTGAAGGC ACAAAACCAT CGTGTCTTGA TTTTCTCCCA GATGACCAAG 
ATGTTGGACA TTCTCGAGGA CTTCCTCGAG GGCGAGCAGT ACAAGTACGA ACGTATTGAC 
GGTGGAATCA CAGGAACATT GCGTCAGGAA GCCATTGATA GGTTCAATGC ACCCGGAGCG 
CAGCAGTTTG TCTTCTTGCT GAGTACTCGA GCCGGAGGAT TGGGTATTAA TTTGGCCACG 
GCCGACACTG TCATTATTTA TGACTCCGAT TGGAATCCCC ACAACGATAT TCAGGCCTTC 
TCCCGAGCCC ATCGTATTGG CCAGGCTAAC AAGGTGATGA TCTATCGATT TGTTACTCGA 
AATTCTGTGG AGGAGCGCGT TACCCAGGTG GCCAAGCGTA AGATGATGTT GACTCATCTT 
GTGGTCCGTC CGGGAATGGG CGGTAAAGGC GCTAACTTTA CAAAGCAAGA ACTGGACGAT 
ATCCTTCGTT TCGGTACCGA GGATCTGTTC AAGGAGGATG ACAAGGAGGA AGCTATCCAC 
TATGACGACA AGGCGGTGGC CGAGCTGCTC GACAGAACCA ACCGCGGTAT CGAGGAGAAG 
GAGTCCTGGG CCAACGAGTA TCTGTCCTCG TTTAAGGTGG CTTCGTACGC CACTAAAGAA 
GAGGAGGAGG AGGAAGAGAC GGAGATTATT AAACAGGATG CCGAGAACTC TGATCCGGCG 
TACTGGGTGA AGCTGCTGCG GCATCACTAT GAACAGCACC AGGAAGATGT GGGTCGCAGC 
CTGGGCAAGG GCAAGCGCGT CCGCAAACAG GTCAACTACA CGGATGGCGG CGTTGTGGCG 
GCTGATACCA CGCGGGATGA TTCCAACTGG CAGGACAACG GCAGCGAGTA TAACTCCGAG 
TATTCAGCTG GCTCCGATGA GGATGGCGGT GACGATGACT TCGATGACCA GAATGGCGCA 
GAGAGAAAGG CCAAGCGGCG ATTGGAGCGT CGCGACGATC GTCCATTGCC TCCACTGTTG 
GCCCGCGTGG GTGGAAACAT CGAGGTGCTC GGTTTTAATG CCCGTCAACG CAAGAGCTTC 
CTCAATGCCA TTATGCGGTA CGGAATGCCG CCGCAAGATG CCTTCAACTC GCAATGGCTA 
GTCCGGGATT TGCGTGGTAA ATCGGAGCGC AATTTTAAGG CGTATGTTTC TCTGTTTATG 
CGGCATCTTT GCGAGCCGGG AGCGGACAAT GCGGAAACCT TTGCCGATGG TGTGCCGCGC 
GAAGGACTAT CCCGCCAGCA TGTGCTCACC CGGATCGGAG TGATGTCGCT AATTCGAAAG 
AAGGTACAGG AATTCGAGCA TATTAACGGA TACTACAGCA TGCCGGAGCT CATTCTCAAG 
CCGTGCGAGC CCGTACGATC AGCCCTGAAA CAGGATGTGG CGGCACTTGA AGCTCCGCCT 
ACCGGTGGCA ATGTCGACAA GAGCGCTACT ACTAGCAATA GCGTCACTCC AGCCACCAGT 
GCGGCTCCCA GTCCAGCGCC AGCCTCTGAA AAAGGTGAAG ACAAAGACAA GGACTCCGAA 
AAGGAGAAGG ATAAGACCTC GGCGGAGAAG AGCGAGGTGA AGCAAGAACA AGAGGCTGAA 
GAGGACAAGA AGCCTGGGGA TGTCAAGCAG GAGAACCCTG TGGAAGAAGC TGCCGGCGAC 
ACAAAGCCCA GCGACGCGGA GGTCAAAACC GAGGTGGCTA AAACAGAACC CAAGGAAGAG 
ACCAAGGACC CGGAGGTAAA GGAGGAGCCC AAGACCGAAG AGAAGGAAAA AGAAAAGGTG 
GATGACAAGA AACCAATACC ACCGACGACA GTAATTGACG ACGACGATGA CGATGTGATG 
ATTGTCAAGG AGGACGGTGA GCTAGAAAAG CCCAGTGCCA GTTCACCCAA GGATCAAAAG 
GCAGTCGCCG CTGCTACGTC TGCTGCAACT GGAGCCACTG GCAAGGGAGC GGAGGACAGC 
TTGGAGGTGC TAAAGCGCAA ATTTATGTTC AACATCGCCG ACGGCGGGTT CACCGAACTG 
CACACTTTGT GGCTCAACGA GGAGAAGGCT GCCGTTCCTG GGCGAGAATA CGAGATCTGG 
CACCGTCGCC ACGACTACTG GCTGTTGGCC GGAATTGTGA CCCATGGCTA TGGCCGTTGG 
CAGGATATAC AAAACGATAT CCGTTTCGCG ATCATAAACG AACCTTTCAA GATGGACGTT 
GGTAAGGGCA ACTTTCTGGA AATCAAAAAC AAGTTCCTGG CCCGGCGTTT TAAGTTGCTG 
GAGCAGGCGC TGGTCATCGA GGAGCAGCTG CGGCGAGCGG CCTACCTTAA CCTCGCCCAG 
GACCCCAGCC ACCCGGCAAT GTCGCTAAAC GCCCGTTTCG CAGAGGTCGA ATGCCTGGCT 
GAATCCCATC AGCATCTGAG CAAGGAATCG CTGGCCGGTA ACAAGCCCGC AAACGCTGTT 
CTGCACAAGG TGCTCAACCA GCTCGAAGAG CTGCTCTCGG ACATGAAGAG CGATGTGTCC 
CGGCTGCCAG CTACACTGGC TCGCATCCCA CCTGTTGCGC AACGGCTTCA GATGTCCGAG 
AGATCGATTC TCTCCCGACT GGCGGCCACC GCCGGAAATG CCTCCAATGC AGCTCAATTG 
ATGGCACAAT TCCCGGCTGG ATTCCAGGGC ACAACATTGC CAGCATTCAC GAGCGGACCG 
GCTGGCAACT TTGCCAACTT TCGGCCACAG TTCTCGGTGC CCGGCCAGCT ATCGAATAAT 
TCCGGCGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014591.1
CDS	137..6088
Translation	MASEEENDDNFQEEEEAQEDNAPAAELSNDSDAPLKPNQNDEDDDYDPEDSRRKKKGKKRKTRKGEEKGRKKKKRKKNESEEDSDFVQHDEEVEYPSTSKRGRKRKEEKQAAKEKESASSGMPSVEDVCSAFSVCNVEIEYSEEELQSLTTYKAFMHHVRPILQKENPKIAAPKLVMLVAAKWREFCESNPHIQQEGGAAGSGGSAGQARSVTGDEPEEPRSSRSSRNEKPDDIYEEAVEEEEEEEEEEKKPRRKRSGRGKKGRRPSGKVPTLKIKLLGKRKRDSSDEEQDASGASERDSDLEFERMLQKSDDSADEKEAPVSSKADNSAPAAQDDGSGAPVVRKKAKTKIGNKFKKKNKLKKTKNFPEGEDGEHEHQDYCEVCQQGGEIILCDTCPRAYHLVCLEPELDEPPEGKWSCPHCEADGGAAEEEDDDEHQEFCRVCKDGGELLCCDSCPSAYHTFCLNPPLDTIPDGDWRCPRCSCPPLTGKAEKIITWRWAQRSNDDGPSTSKGSKNSNSRVREYFIKWHNMSYWHCEWVPEVQLDVHHPLMIRSFQRKYDMEEPPKFEESLDEADTRYKRIQRHKDKVGMKANDDAEVLEERFYKNGVKPEWLIVQRVINHRTARDGSTMYLVKWRELPYDKSTWEEEGDDIQGLRQAIDYYQDLRAVCTSETTQSRSKKSKKGRKSKLKVEDDEDRPVKHYTPPPEKPTTDLKKKYEDQPAFLEGTGMQLHPYQIEGINWLRYSWGQGIDTILADEMGLGKTIQTVTFLYSLYKEGHCRGPFLVAVPLSTLVNWEREFELWAPDFYCITYIGDKDSRAVIRENELSFEEGAIRGSKVSRLRTTQYKFNVLLTSYELISMDAACLGSIDWAVLVVDEAHRLKSNQSKFFRILNSYTIAYKLLLTGTPLQNNLEELFHLLNFLSRDKFNDLQAFQGEFADVSKEEQVKRLHEMLGPHMLRRLKTDVLKNMPSKSEFIVRVELSAMQKKFYKFILTKNYEALNSKSGGGSCSLINIMMDLKKCCNHPYLFPSAAEEATTAAGGLYEINSLTKAAGKLVLLSKMLKQLKAQNHRVLIFSQMTKMLDILEDFLEGEQYKYERIDGGITGTLRQEAIDRFNAPGAQQFVFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQANKVMIYRFVTRNSVEERVTQVAKRKMMLTHLVVRPGMGGKGANFTKQELDDILRFGTEDLFKEDDKEEAIHYDDKAVAELLDRTNRGIEEKESWANEYLSSFKVASYATKEEEEEEETEIIKQDAENSDPAYWVKLLRHHYEQHQEDVGRSLGKGKRVRKQVNYTDGGVVAADTTRDDSNWQDNGSEYNSEYSAGSDEDGGDDDFDDQNGAERKAKRRLERRDDRPLPPLLARVGGNIEVLGFNARQRKSFLNAIMRYGMPPQDAFNSQWLVRDLRGKSERNFKAYVSLFMRHLCEPGADNAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHINGYYSMPELILKPCEPVRSALKQDVAALEAPPTGGNVDKSATTSNSVTPATSAAPSPAPASEKGEDKDKDSEKEKDKTSAEKSEVKQEQEAEEDKKPGDVKQENPVEEAAGDTKPSDAEVKTEVAKTEPKEETKDPEVKEEPKTEEKEKEKVDDKKPIPPTTVIDDDDDDVMIVKEDGELEKPSASSPKDQKAVAAATSAATGATGKGAEDSLEVLKRKFMFNIADGGFTELHTLWLNEEKAAVPGREYEIWHRRHDYWLLAGIVTHGYGRWQDIQNDIRFAIINEPFKMDVGKGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNLAQDPSHPAMSLNARFAEVECLAESHQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKSDVSRLPATLARIPPVAQRLQMSERSILSRLAATAGNASNAAQLMAQFPAGFQGTTLPAFTSGPAGNFANFRPQFSVPGQLSNNSGV

Target ORF information:

RefSeq Version	NM_001014591.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Mi-2, transcript variant B (Mi-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014591.3

ORF Insert Sequence:

ATGGCATCGG AGGAAGAGAA TGACGATAAT TTCCAGGAAG AAGAAGAGGC CCAGGAGGAC 
AACGCACCTG CGGCGGAGCT GTCCAACGAT TCCGACGCCC CTCTGAAGCC GAATCAGAAC 
GACGAAGACG ACGACTACGA TCCGGAGGAC AGTCGCAGGA AGAAGAAGGG CAAGAAGCGC 
AAGACCAGGA AGGGCGAAGA GAAGGGTCGC AAGAAGAAGA AGCGCAAGAA GAACGAGAGC 
GAAGAGGACA GCGACTTTGT CCAGCACGAT GAGGAGGTGG AGTACCCCAG CACCTCCAAG 
CGCGGTCGCA AGCGCAAGGA GGAGAAACAG GCCGCCAAGG AGAAGGAGTC GGCATCATCC 
GGAATGCCAT CTGTGGAAGA CGTGTGCTCA GCCTTTAGCG TCTGCAATGT GGAGATCGAG 
TACAGTGAGG AGGAGCTGCA GAGCCTAACG ACCTACAAGG CTTTCATGCA TCATGTTCGT 
CCGATTCTGC AGAAGGAAAA CCCCAAGATT GCGGCACCAA AGCTAGTGAT GCTGGTGGCA 
GCCAAGTGGC GTGAGTTCTG CGAGAGCAAT CCGCACATCC AGCAGGAGGG TGGTGCCGCC 
GGCTCCGGTG GATCCGCCGG ACAGGCACGC AGTGTGACAG GCGATGAGCC GGAGGAACCT 
CGATCATCGC GTTCCTCGCG CAACGAGAAA CCGGATGACA TTTACGAGGA GGCCGTCGAG 
GAGGAGGAAG AAGAGGAGGA GGAGGAGAAG AAACCTCGTC GTAAGCGCAG TGGACGTGGC 
AAGAAGGGGC GTCGTCCCTC CGGCAAGGTG CCCACTCTTA AGATTAAGCT GCTCGGCAAG 
CGCAAACGCG ATAGTTCGGA TGAGGAGCAA GATGCTAGCG GTGCATCGGA ACGCGATTCG 
GATCTTGAGT TTGAACGGAT GCTTCAGAAA TCGGATGACA GTGCCGATGA AAAGGAGGCT 
CCTGTCTCCT CTAAGGCGGA CAACTCAGCA CCCGCTGCCC AGGACGATGG ATCAGGAGCT 
CCAGTGGTGC GCAAGAAGGC CAAGACCAAG ATCGGCAACA AGTTCAAGAA GAAGAATAAA 
CTCAAGAAAA CGAAGAACTT CCCGGAGGGC GAAGATGGCG AGCATGAGCA CCAGGACTAC 
TGCGAGGTGT GCCAGCAAGG AGGTGAGATC ATCCTGTGCG ACACCTGCCC TCGGGCATAT 
CACTTGGTGT GCCTGGAGCC AGAACTCGAT GAACCGCCAG AGGGCAAGTG GTCGTGTCCG 
CACTGCGAGG CTGACGGAGG TGCTGCTGAG GAAGAGGACG ATGATGAGCA CCAGGAATTC 
TGCCGCGTGT GCAAGGACGG TGGCGAGTTG CTGTGCTGCG ACTCATGTCC CTCCGCCTAT 
CACACATTCT GCTTGAATCC GCCTCTGGAC ACTATACCCG ATGGCGATTG GCGCTGTCCT 
CGTTGCAGCT GCCCTCCGCT CACTGGCAAG GCTGAGAAGA TCATCACCTG GCGATGGGCA 
CAGCGTAGCA ATGACGACGG TCCCTCCACC TCGAAGGGAT CAAAGAACAG TAACTCCCGG 
GTGCGCGAGT ACTTTATCAA GTGGCACAAC ATGTCCTACT GGCATTGCGA ATGGGTGCCC 
GAAGTGCAAC TGGACGTCCA TCATCCGCTC ATGATTCGTT CGTTCCAGCG CAAGTACGAC 
ATGGAAGAAC CGCCCAAGTT CGAGGAGTCG CTGGATGAGG CGGACACTCG TTACAAGCGT 
ATTCAGCGAC ACAAGGACAA GGTTGGCATG AAGGCAAACG ATGATGCCGA AGTGCTGGAG 
GAGCGGTTCT ACAAGAATGG CGTCAAGCCA GAGTGGCTCA TTGTTCAGCG CGTAATCAAC 
CATCGTACGG CAAGAGATGG CAGCACCATG TATCTGGTGA AGTGGCGCGA GTTGCCCTAT 
GACAAGTCCA CCTGGGAAGA AGAGGGTGAT GACATACAGG GCCTGCGGCA AGCCATCGAT 
TACTATCAGG ATCTGCGTGC GGTTTGCACC TCGGAGACAA CTCAGTCGCG CAGCAAAAAG 
AGCAAGAAGG GTCGCAAGTC CAAGCTCAAG GTCGAGGACG ACGAGGATCG CCCGGTCAAG 
CACTACACAC CGCCGCCGGA AAAGCCAACT ACTGATCTGA AGAAGAAGTA TGAGGATCAG 
CCGGCTTTCT TGGAGGGCAC TGGCATGCAA TTGCATCCCT ACCAGATCGA GGGCATTAAC 
TGGTTGCGCT ACAGCTGGGG CCAGGGCATC GACACCATCC TGGCCGACGA GATGGGTCTG 
GGTAAGACCA TTCAGACGGT CACCTTCCTG TACTCTTTGT ACAAGGAGGG CCATTGCCGC 
GGACCCTTCC TCGTGGCCGT GCCACTCTCG ACGCTAGTGA ACTGGGAGCG TGAGTTTGAG 
CTGTGGGCTC CGGACTTTTA CTGCATCACG TATATCGGTG ACAAGGACTC GAGAGCGGTT 
ATACGGGAGA ACGAGCTGAG CTTCGAGGAG GGCGCCATTC GTGGTAGCAA GGTTTCGCGT 
TTGCGGACCA CCCAGTATAA ATTCAACGTA CTGCTGACTA GCTACGAGCT GATCTCCATG 
GACGCTGCTT GCCTTGGCAG CATTGACTGG GCCGTGCTCG TGGTGGACGA GGCCCATCGC 
TTGAAGAGCA ACCAGAGCAA GTTCTTCCGT ATCCTGAACA GCTACACTAT TGCCTACAAG 
CTTCTGCTCA CCGGCACACC GCTGCAGAAC AATCTCGAGG AGCTGTTCCA TCTGCTCAAC 
TTCTTGAGCC GCGACAAGTT CAATGATCTG CAGGCCTTCC AGGGCGAATT CGCTGACGTT 
TCGAAGGAGG AGCAGGTTAA GCGTCTGCAC GAGATGCTTG GACCGCACAT GCTGCGTCGT 
TTGAAGACGG ATGTGCTGAA GAACATGCCG TCCAAGTCTG AGTTTATCGT GCGCGTCGAG 
CTGTCGGCCA TGCAGAAAAA GTTCTACAAG TTCATCTTGA CCAAGAACTA CGAGGCTTTG 
AATTCGAAGA GCGGTGGTGG CTCTTGCTCA CTGATCAACA TTATGATGGA CCTAAAGAAG 
TGCTGCAACC ATCCGTATCT CTTCCCCTCG GCCGCCGAGG AGGCAACCAC CGCTGCCGGT 
GGTCTCTACG AGATTAACTC GCTGACCAAG GCTGCTGGCA AGCTGGTCCT GCTATCCAAG 
ATGCTGAAGC AGCTGAAGGC ACAAAACCAT CGTGTCTTGA TTTTCTCCCA GATGACCAAG 
ATGTTGGACA TTCTCGAGGA CTTCCTCGAG GGCGAGCAGT ACAAGTACGA ACGTATTGAC 
GGTGGAATCA CAGGAACATT GCGTCAGGAA GCCATTGATA GGTTCAATGC ACCCGGAGCG 
CAGCAGTTTG TCTTCTTGCT GAGTACTCGA GCCGGAGGAT TGGGTATTAA TTTGGCCACG 
GCCGACACTG TCATTATTTA TGACTCCGAT TGGAATCCCC ACAACGATAT TCAGGCCTTC 
TCCCGAGCCC ATCGTATTGG CCAGGCTAAC AAGGTGATGA TCTATCGATT TGTTACTCGA 
AATTCTGTGG AGGAGCGCGT TACCCAGGTG GCCAAGCGTA AGATGATGTT GACTCATCTT 
GTGGTCCGTC CGGGAATGGG CGGTAAAGGC GCTAACTTTA CAAAGCAAGA ACTGGACGAT 
ATCCTTCGTT TCGGTACCGA GGATCTGTTC AAGGAGGATG ACAAGGAGGA AGCTATCCAC 
TATGACGACA AGGCGGTGGC CGAGCTGCTC GACAGAACCA ACCGCGGTAT CGAGGAGAAG 
GAGTCCTGGG CCAACGAGTA TCTGTCCTCG TTTAAGGTGG CTTCGTACGC CACTAAAGAA 
GAGGAGGAGG AGGAAGAGAC GGAGATTATT AAACAGGATG CCGAGAACTC TGATCCGGCG 
TACTGGGTGA AGCTGCTGCG GCATCACTAT GAACAGCACC AGGAAGATGT GGGTCGCAGC 
CTGGGCAAGG GCAAGCGCGT CCGCAAACAG GTCAACTACA CGGATGGCGG CGTTGTGGCG 
GCTGATACCA CGCGGGATGA TTCCAACTGG CAGGACAACG GCAGCGAGTA TAACTCCGAG 
TATTCAGCTG GCTCCGATGA GGATGGCGGT GACGATGACT TCGATGACCA GAATGGCGCA 
GAGAGAAAGG CCAAGCGGCG ATTGGAGCGT CGCGACGATC GTCCATTGCC TCCACTGTTG 
GCCCGCGTGG GTGGAAACAT CGAGGTGCTC GGTTTTAATG CCCGTCAACG CAAGAGCTTC 
CTCAATGCCA TTATGCGGTA CGGAATGCCG CCGCAAGATG CCTTCAACTC GCAATGGCTA 
GTCCGGGATT TGCGTGGTAA ATCGGAGCGC AATTTTAAGG CGTATGTTTC TCTGTTTATG 
CGGCATCTTT GCGAGCCGGG AGCGGACAAT GCGGAAACCT TTGCCGATGG TGTGCCGCGC 
GAAGGACTAT CCCGCCAGCA TGTGCTCACC CGGATCGGAG TGATGTCGCT AATTCGAAAG 
AAGGTACAGG AATTCGAGCA TATTAACGGA TACTACAGCA TGCCGGAGCT CATTCTCAAG 
CCGTGCGAGC CCGTACGATC AGCCCTGAAA CAGGATGTGG CGGCACTTGA AGCTCCGCCT 
ACCGGTGGCA ATGTCGACAA GAGCGCTACT ACTAGCAATA GCGTCACTCC AGCCACCAGT 
GCGGCTCCCA GTCCAGCGCC AGCCTCTGAA AAAGGTGAAG ACAAAGACAA GGACTCCGAA 
AAGGAGAAGG ATAAGACCTC GGCGGAGAAG AGCGAGGTGA AGCAAGAACA AGAGGCTGAA 
GAGGACAAGA AGCCTGGGGA TGTCAAGCAG GAGAACCCTG TGGAAGAAGC TGCCGGCGAC 
ACAAAGCCCA GCGACGCGGA GGTCAAAACC GAGGTGGCTA AAACAGAACC CAAGGAAGAG 
ACCAAGGACC CGGAGGTAAA GGAGGAGCCC AAGACCGAAG AGAAGGAAAA AGAAAAGGTG 
GATGACAAGA AACCAATACC ACCGACGACA GTAATTGACG ACGACGATGA CGATGTGATG 
ATTGTCAAGG AGGACGGTGA GCTAGAAAAG CCCAGTGCCA GTTCACCCAA GGATCAAAAG 
GCAGTCGCCG CTGCTACGTC TGCTGCAACT GGAGCCACTG GCAAGGGAGC GGAGGACAGC 
TTGGAGGTGC TAAAGCGCAA ATTTATGTTC AACATCGCCG ACGGCGGGTT CACCGAACTG 
CACACTTTGT GGCTCAACGA GGAGAAGGCT GCCGTTCCTG GGCGAGAATA CGAGATCTGG 
CACCGTCGCC ACGACTACTG GCTGTTGGCC GGAATTGTGA CCCATGGCTA TGGCCGTTGG 
CAGGATATAC AAAACGATAT CCGTTTCGCG ATCATAAACG AACCTTTCAA GATGGACGTT 
GGTAAGGGCA ACTTTCTGGA AATCAAAAAC AAGTTCCTGG CCCGGCGTTT TAAGTTGCTG 
GAGCAGGCGC TGGTCATCGA GGAGCAGCTG CGGCGAGCGG CCTACCTTAA CCTCGCCCAG 
GACCCCAGCC ACCCGGCAAT GTCGCTAAAC GCCCGTTTCG CAGAGGTCGA ATGCCTGGCT 
GAATCCCATC AGCATCTGAG CAAGGAATCG CTGGCCGGTA ACAAGCCCGC AAACGCTGTT 
CTGCACAAGG TGCTCAACCA GCTCGAAGAG CTGCTCTCGG ACATGAAGAG CGATGTGTCC 
CGGCTGCCAG CTACACTGGC TCGCATCCCA CCTGTTGCGC AACGGCTTCA GATGTCCGAG 
AGATCGATTC TCTCCCGACT GGCGGCCACC GCCGGAAATG CCTCCAATGC AGCTCAATTG 
ATGGCACAAT TCCCGGCTGG ATTCCAGGGC ACAACATTGC CAGCATTCAC GAGCGGACCG 
GCTGGCAACT TTGCCAACTT TCGGCCACAG TTCTCGGTGC CCGGCCAGCT ATCGAATAAT 
TCCGGCGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mi-2 ( NM_001275149.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262078.1 Drosophila melanogaster Mi-2, transcript variant D (Mi-2), mRNA.

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CloneID	OFa21108
Clone ID Related Accession (Same CDS sequence)	NM_001275149.1
Accession Version	NM_001275149.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Mi-2, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCACG AAGAAGAAGA GGCCCAGGAG GACAACGCAC CTGCGGCGGA GCTGTCCAAC 
GATTCCGACG CCCCTCTGAA GCCGAATAAC GACGAAGACG ACGACTACGA TCCGGAGGAC 
AGTCGCAGGA AGAAGAAGGG CAAGAAGCGC AAGACCAGGA AGGGCGAAGA GAAGGGTCGC 
AAGAAGAAGA AGCGCAAGAA GAACGAGAGC GAAGAGGACA GCGACTTTGT CCAGCACGAT 
GAGGAGGTGG AGTACCCCAG CACCTCCAAG CGCGGTCGCA AGCGCAAGGA GGAGAAACAG 
GCCGCCAAGG AGAAGGAGTC GGCATCATCC GGAATGCCAT CTGTGGAAGA CGTGTGCTCA 
GCCTTTAGCG TCTGCAATGT GGAGATCGAG TACAGTGAGG AGGAGCTGCA GAGCCTAACG 
ACCTACAAGG CTTTCATGCA TCATGTTCGT CCGATTCTGC AGAAGGAAAA CCCCAAGATT 
GCGGCACCAA AGCTAGTGAT GCTGGTGGCA GCCAAGTGGC GTGAGTTCTG CGAGAGCAAT 
CCGCACATCC AGCAGGAGGG TGGTGCCGCC GGCTCCGGTG GATCCGCCGG ACAGGCACGC 
AGTGTGACAG GCGATGAGCC GGAGGAACCT CGATCATCGC GTTCCTCGCG CAACGAGAAA 
CCGGATGACA TTTACGAGGA GGCCGTCGAG GAGGAGGAAG AAGAGGAGGA GGAGGAGAAG 
AAACCTCGTC GTAAGCGCAG TGGACGTGGC AAGAAGGGGC GTCGTCCCTC CGGCAAGGTG 
CCCACTCTTA AGATTAAGCT GCTCGGCAAG CGCAAACGCG ATAGTTCGGA TGAGGAGCAA 
GATGCTAGCG GTGCATCGGA ACGCGATTCG GATCTTGAGT TTGAACGGAT GCTTCAGAAA 
TCGGATGACA GTGCCGATGA AAAGGAGGCT CCTGTCTCCT CTAAGGCGGA CAACTCAGCA 
CCCGCTGCCC AGGACGATGG ATCAGGAGCT CCAGTGGTGC GCAAGAAGGC CAAGACCAAG 
ATCGGCAACA AGTTCAAGAA GAAGAATAAA CTCAAGAAAA CGAAGAACTT CCCGGAGGGC 
GAAGATGGCG AGCATGAGCA CCAGGACTAC TGCGAGGTGT GCCAGCAAGG AGGTGAGATC 
ATCCTGTGCG ACACCTGCCC TCGGGCATAT CACTTGGTGT GCCTGGAGCC AGAACTCGAT 
GAACCGCCAG AGGGCAAGTG GTCGTGTCCG CACTGCGAGG CTGACGGAGG TGCTGCTGAG 
GAAGAGGACG ATGATGAGCA CCAGGAATTC TGCCGCGTGT GCAAGGACGG TGGCGAGTTG 
CTGTGCTGCG ACTCATGTCC CTCCGCCTAT CACACATTCT GCTTGAATCC GCCTCTGGAC 
ACTATACCCG ATGGCGATTG GCGCTGTCCT CGTTGCAGCT GCCCTCCGCT CACTGGCAAG 
GCTGAGAAGA TCATCACCTG GCGATGGGCA CAGCGTAGCA ATGACGACGG TCCCTCCACC 
TCGAAGGGAT CAAAGAACAG TAACTCCCGG GTGCGCGAGT ACTTTATCAA GTGGCACAAC 
ATGTCCTACT GGCATTGCGA ATGGGTGCCC GAAGTGCAAC TGGACGTCCA TCATCCGCTC 
ATGATTCGTT CGTTCCAGCG CAAGTACGAC ATGGAAGAAC CGCCCAAGTT CGAGGAGTCG 
CTGGATGAGG CGGACACTCG TTACAAGCGT ATTCAGCGAC ACAAGGACAA GGTTGGCATG 
AAGGCAAACG ATGATGCCGA AGTGCTGGAG GAGCGGTTCT ACAAGAATGG CGTCAAGCCA 
GAGTGGCTCA TTGTTCAGCG CGTAATCAAC CATCGTACGG CAAGAGATGG CAGCACCATG 
TATCTGGTGA AGTGGCGCGA GTTGCCCTAT GACAAGTCCA CCTGGGAAGA AGAGGGTGAT 
GACATACAGG GCCTGCGGCA AGCCATCGAT TACTATCAGG ATCTGCGTGC GGTTTGCACC 
TCGGAGACAA CTCAGTCGCG CAGCAAAAAG AGCAAGAAGG GTCGCAAGTC CAAGCTCAAG 
GTCGAGGACG ACGAGGATCG CCCGGTCAAG CACTACACAC CGCCGCCGGA AAAGCCAACT 
ACTGATCTGA AGAAGAAGTA TGAGGATCAG CCGGCTTTCT TGGAGGGCAC TGGCATGCAA 
TTGCATCCCT ACCAGATCGA GGGCATTAAC TGGTTGCGCT ACAGCTGGGG CCAGGGCATC 
GACACCATCC TGGCCGACGA GATGGGTCTG GGTAAGACCA TTCAGACGGT CACCTTCCTG 
TACTCTTTGT ACAAGGAGGG CCATTGCCGC GGACCCTTCC TCGTGGCCGT GCCACTCTCG 
ACGCTAGTGA ACTGGGAGCG TGAGTTTGAG CTGTGGGCTC CGGACTTTTA CTGCATCACG 
TATATCGGTG ACAAGGACTC GAGAGCGGTT ATACGGGAGA ACGAGCTGAG CTTCGAGGAG 
GGCGCCATTC GTGGTAGCAA GGTTTCGCGT TTGCGGACCA CCCAGTATAA ATTCAACGTA 
CTGCTGACTA GCTACGAGCT GATCTCCATG GACGCTGCTT GCCTTGGCAG CATTGACTGG 
GCCGTGCTCG TGGTGGACGA GGCCCATCGC TTGAAGAGCA ACCAGAGCAA GTTCTTCCGT 
ATCCTGAACA GCTACACTAT TGCCTACAAG CTTCTGCTCA CCGGCACACC GCTGCAGAAC 
AATCTCGAGG AGCTGTTCCA TCTGCTCAAC TTCTTGAGCC GCGACAAGTT CAATGATCTG 
CAGGCCTTCC AGGGCGAATT CGCTGACGTT TCGAAGGAGG AGCAGGTTAA GCGTCTGCAC 
GAGATGCTTG GACCGCACAT GCTGCGTCGT TTGAAGACGG ATGTGCTGAA GAACATGCCG 
TCCAAGTCTG AGTTTATCGT GCGCGTCGAG CTGTCGGCCA TGCAGAAAAA GTTCTACAAG 
TTCATCTTGA CCAAGAACTA CGAGGCTTTG AATTCGAAGA GCGGTGGTGG CTCTTGCTCA 
CTGATCAACA TTATGATGGA CCTAAAGAAG TGCTGCAACC ATCCGTATCT CTTCCCCTCG 
GCCGCCGAGG AGGCAACCAC CGCTGCCGGT GGTCTCTACG AGATTAACTC GCTGACCAAG 
GCTGCTGGCA AGCTGGTCCT GCTATCCAAG ATGCTGAAGC AGCTGAAGGC ACAAAACCAT 
CGTGTCTTGA TTTTCTCCCA GATGACCAAG ATGTTGGACA TTCTCGAGGA CTTCCTCGAG 
GGCGAGCAGT ACAAGTACGA ACGTATTGAC GGTGGAATCA CAGGAACATT GCGTCAGGAA 
GCCATTGATA GGTTCAATGC ACCCGGAGCG CAGCAGTTTG TCTTCTTGCT GAGTACTCGA 
GCCGGAGGAT TGGGTATTAA TTTGGCCACG GCCGACACTG TCATTATTTA TGACTCCGAT 
TGGAATCCCC ACAACGATAT TCAGGCCTTC TCCCGAGCCC ATCGTATTGG CCAGGCTAAC 
AAGGTGATGA TCTATCGATT TGTTACTCGA AATTCTGTGG AGGAGCGCGT TACCCAGGTG 
GCCAAGCGTA AGATGATGTT GACTCATCTT GTGGTCCGTC CGGGAATGGG CGGTAAAGGC 
GCTAACTTTA CAAAGCAAGA ACTGGACGAT ATCCTTCGTT TCGGTACCGA GGATCTGTTC 
AAGGAGGATG ACAAGGAGGA AGCTATCCAC TATGACGACA AGGCGGTGGC CGAGCTGCTC 
GACAGAACCA ACCGCGGTAT CGAGGAGAAG GAGTCCTGGG CCAACGAGTA TCTGTCCTCG 
TTTAAGGTGG CTTCGTACGC CACTAAAGAA GAGGAGGAGG AGGAAGAGAC GGAGATTATT 
AAACAGGATG CCGAGAACTC TGATCCGGCG TACTGGGTGA AGCTGCTGCG GCATCACTAT 
GAACAGCACC AGGAAGATGT GGGTCGCAGC CTGGGCAAGG GCAAGCGCGT CCGCAAACAG 
GTCAACTACA CGGATGGCGG CGTTGTGGCG GCTGATACCA CGCGGGATGA TTCCAACTGG 
CAGGACAACG GCAGCGAGTA TAACTCCGAG TATTCAGCTG GCTCCGATGA GGATGGCGGT 
GACGATGACT TCGATGACCA GAATGGCGCA GAGAGAAAGG CCAAGCGGCG ATTGGAGCGT 
CGCGACGATC GTCCATTGCC TCCACTGTTG GCCCGCGTGG GTGGAAACAT CGAGGTGCTC 
GGTTTTAATG CCCGTCAACG CAAGAGCTTC CTCAATGCCA TTATGCGGTA CGGAATGCCG 
CCGCAAGATG CCTTCAACTC GCAATGGCTA GTCCGGGATT TGCGTGGTAA ATCGGAGCGC 
AATTTTAAGG CGTATGTTTC TCTGTTTATG CGGCATCTTT GCGAGCCGGG AGCGGACAAT 
GCGGAAACCT TTGCCGATGG TGTGCCGCGC GAAGGACTAT CCCGCCAGCA TGTGCTCACC 
CGGATCGGAG TGATGTCGCT AATTCGAAAG AAGGTACAGG AATTCGAGCA TATTAACGGA 
TACTACAGCA TGCCGGAGCT CATTCTCAAG CCGTGCGAGC CCGTACGATC AGCCCTGAAA 
CAGGATGTGG CGGCACTTGA AGCTCCGCCT ACCGGTGGCA ATGTCGACAA GAGCGCTACT 
ACTAGCAATA GCGTCACTCC AGCCACCAGT GCGGCTCCCA GTCCAGCGCC AGCCTCTGAA 
AAAGGTGAAG ACAAAGACAA GGACTCCGAA AAGGAGAAGG ATAAGACCTC GGCGGAGAAG 
AGCGAGGTGA AGCAAGAACA AGAGGCTGAA GAGGACAAGA AGCCTGGGGA TGTCAAGCAG 
GAGAACCCTG TGGAAGAAGC TGCCGGCGAC ACAAAGCCCA GCGACGCGGA GGTCAAAACC 
GAGGTGGCTA AAACAGAACC CAAGGAAGAG ACCAAGGACC CGGAGGTAAA GGAGGAGCCC 
AAGACCGAAG AGAAGGAAAA AGAAAAGGTG GATGACAAGA AACCAATACC ACCGACGACA 
GTAATTGACG ACGACGATGA CGATGTGATG ATTGTCAAGG AGGACGGTGA GCTAGAAAAG 
CCCAGTGCCA GTTCACCCAA GGATCAAAAG GCAGTCGCCG CTGCTACGTC TGCTGCAACT 
GGAGCCACTG GCAAGGGAGC GGAGGACAGC TTGGAGGTGC TAAAGCGCAA ATTTATGTTC 
AACATCGCCG ACGGCGGGTT CACCGAACTG CACACTTTGT GGCTCAACGA GGAGAAGGCT 
GCCGTTCCTG GGCGAGAATA CGAGATCTGG CACCGTCGCC ACGACTACTG GCTGTTGGCC 
GGAATTGTGA CCCATGGCTA TGGCCGTTGG CAGGATATAC AAAACGATAT CCGTTTCGCG 
ATCATAAACG AACCTTTCAA GATGGACGTT GGTAAGGGCA ACTTTCTGGA AATCAAAAAC 
AAGTTCCTGG CCCGGCGTTT TAAGTTGCTG GAGCAGGCGC TGGTCATCGA GGAGCAGCTG 
CGGCGAGCGG CCTACCTTAA CCTCGCCCAG GACCCCAGCC ACCCGGCAAT GTCGCTAAAC 
GCCCGTTTCG CAGAGGTCGA ATGCCTGGCT GAATCCCATC AGCATCTGAG CAAGGAATCG 
CTGGCCGGTA ACAAGCCCGC AAACGCTGTT CTGCACAAGG TGCTCAACCA GCTCGAAGAG 
CTGCTCTCGG ACATGAAGAG CGATGTGTCC CGGCTGCCAG CTACACTGGC TCGCATCCCA 
CCTGTTGCGC AACGGCTTCA GATGTCCGAG AGATCGATTC TCTCCCGACT GGCGGCCACC 
GCCGGAAATG CCTCCAATGC AGCTCAATTG ATGGCACAAT TCCCGGCTGG ATTCCAGGGC 
ACAACATTGC CAGCATTCAC GAGCGGACCG GCTGGCAACT TTGCCAACTT TCGGCCACAG 
TTCTCGGTGC CCGGCCAGCT ATCGAATAAT TCCGGCGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262078.1
CDS	105..6026
Translation	MAHEEEEAQEDNAPAAELSNDSDAPLKPNNDEDDDYDPEDSRRKKKGKKRKTRKGEEKGRKKKKRKKNESEEDSDFVQHDEEVEYPSTSKRGRKRKEEKQAAKEKESASSGMPSVEDVCSAFSVCNVEIEYSEEELQSLTTYKAFMHHVRPILQKENPKIAAPKLVMLVAAKWREFCESNPHIQQEGGAAGSGGSAGQARSVTGDEPEEPRSSRSSRNEKPDDIYEEAVEEEEEEEEEEKKPRRKRSGRGKKGRRPSGKVPTLKIKLLGKRKRDSSDEEQDASGASERDSDLEFERMLQKSDDSADEKEAPVSSKADNSAPAAQDDGSGAPVVRKKAKTKIGNKFKKKNKLKKTKNFPEGEDGEHEHQDYCEVCQQGGEIILCDTCPRAYHLVCLEPELDEPPEGKWSCPHCEADGGAAEEEDDDEHQEFCRVCKDGGELLCCDSCPSAYHTFCLNPPLDTIPDGDWRCPRCSCPPLTGKAEKIITWRWAQRSNDDGPSTSKGSKNSNSRVREYFIKWHNMSYWHCEWVPEVQLDVHHPLMIRSFQRKYDMEEPPKFEESLDEADTRYKRIQRHKDKVGMKANDDAEVLEERFYKNGVKPEWLIVQRVINHRTARDGSTMYLVKWRELPYDKSTWEEEGDDIQGLRQAIDYYQDLRAVCTSETTQSRSKKSKKGRKSKLKVEDDEDRPVKHYTPPPEKPTTDLKKKYEDQPAFLEGTGMQLHPYQIEGINWLRYSWGQGIDTILADEMGLGKTIQTVTFLYSLYKEGHCRGPFLVAVPLSTLVNWEREFELWAPDFYCITYIGDKDSRAVIRENELSFEEGAIRGSKVSRLRTTQYKFNVLLTSYELISMDAACLGSIDWAVLVVDEAHRLKSNQSKFFRILNSYTIAYKLLLTGTPLQNNLEELFHLLNFLSRDKFNDLQAFQGEFADVSKEEQVKRLHEMLGPHMLRRLKTDVLKNMPSKSEFIVRVELSAMQKKFYKFILTKNYEALNSKSGGGSCSLINIMMDLKKCCNHPYLFPSAAEEATTAAGGLYEINSLTKAAGKLVLLSKMLKQLKAQNHRVLIFSQMTKMLDILEDFLEGEQYKYERIDGGITGTLRQEAIDRFNAPGAQQFVFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQANKVMIYRFVTRNSVEERVTQVAKRKMMLTHLVVRPGMGGKGANFTKQELDDILRFGTEDLFKEDDKEEAIHYDDKAVAELLDRTNRGIEEKESWANEYLSSFKVASYATKEEEEEEETEIIKQDAENSDPAYWVKLLRHHYEQHQEDVGRSLGKGKRVRKQVNYTDGGVVAADTTRDDSNWQDNGSEYNSEYSAGSDEDGGDDDFDDQNGAERKAKRRLERRDDRPLPPLLARVGGNIEVLGFNARQRKSFLNAIMRYGMPPQDAFNSQWLVRDLRGKSERNFKAYVSLFMRHLCEPGADNAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHINGYYSMPELILKPCEPVRSALKQDVAALEAPPTGGNVDKSATTSNSVTPATSAAPSPAPASEKGEDKDKDSEKEKDKTSAEKSEVKQEQEAEEDKKPGDVKQENPVEEAAGDTKPSDAEVKTEVAKTEPKEETKDPEVKEEPKTEEKEKEKVDDKKPIPPTTVIDDDDDDVMIVKEDGELEKPSASSPKDQKAVAAATSAATGATGKGAEDSLEVLKRKFMFNIADGGFTELHTLWLNEEKAAVPGREYEIWHRRHDYWLLAGIVTHGYGRWQDIQNDIRFAIINEPFKMDVGKGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNLAQDPSHPAMSLNARFAEVECLAESHQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKSDVSRLPATLARIPPVAQRLQMSERSILSRLAATAGNASNAAQLMAQFPAGFQGTTLPAFTSGPAGNFANFRPQFSVPGQLSNNSGV

Target ORF information:

RefSeq Version	NM_001275149.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Mi-2, transcript variant D (Mi-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275149.1

ORF Insert Sequence:

ATGGCTCACG AAGAAGAAGA GGCCCAGGAG GACAACGCAC CTGCGGCGGA GCTGTCCAAC 
GATTCCGACG CCCCTCTGAA GCCGAATAAC GACGAAGACG ACGACTACGA TCCGGAGGAC 
AGTCGCAGGA AGAAGAAGGG CAAGAAGCGC AAGACCAGGA AGGGCGAAGA GAAGGGTCGC 
AAGAAGAAGA AGCGCAAGAA GAACGAGAGC GAAGAGGACA GCGACTTTGT CCAGCACGAT 
GAGGAGGTGG AGTACCCCAG CACCTCCAAG CGCGGTCGCA AGCGCAAGGA GGAGAAACAG 
GCCGCCAAGG AGAAGGAGTC GGCATCATCC GGAATGCCAT CTGTGGAAGA CGTGTGCTCA 
GCCTTTAGCG TCTGCAATGT GGAGATCGAG TACAGTGAGG AGGAGCTGCA GAGCCTAACG 
ACCTACAAGG CTTTCATGCA TCATGTTCGT CCGATTCTGC AGAAGGAAAA CCCCAAGATT 
GCGGCACCAA AGCTAGTGAT GCTGGTGGCA GCCAAGTGGC GTGAGTTCTG CGAGAGCAAT 
CCGCACATCC AGCAGGAGGG TGGTGCCGCC GGCTCCGGTG GATCCGCCGG ACAGGCACGC 
AGTGTGACAG GCGATGAGCC GGAGGAACCT CGATCATCGC GTTCCTCGCG CAACGAGAAA 
CCGGATGACA TTTACGAGGA GGCCGTCGAG GAGGAGGAAG AAGAGGAGGA GGAGGAGAAG 
AAACCTCGTC GTAAGCGCAG TGGACGTGGC AAGAAGGGGC GTCGTCCCTC CGGCAAGGTG 
CCCACTCTTA AGATTAAGCT GCTCGGCAAG CGCAAACGCG ATAGTTCGGA TGAGGAGCAA 
GATGCTAGCG GTGCATCGGA ACGCGATTCG GATCTTGAGT TTGAACGGAT GCTTCAGAAA 
TCGGATGACA GTGCCGATGA AAAGGAGGCT CCTGTCTCCT CTAAGGCGGA CAACTCAGCA 
CCCGCTGCCC AGGACGATGG ATCAGGAGCT CCAGTGGTGC GCAAGAAGGC CAAGACCAAG 
ATCGGCAACA AGTTCAAGAA GAAGAATAAA CTCAAGAAAA CGAAGAACTT CCCGGAGGGC 
GAAGATGGCG AGCATGAGCA CCAGGACTAC TGCGAGGTGT GCCAGCAAGG AGGTGAGATC 
ATCCTGTGCG ACACCTGCCC TCGGGCATAT CACTTGGTGT GCCTGGAGCC AGAACTCGAT 
GAACCGCCAG AGGGCAAGTG GTCGTGTCCG CACTGCGAGG CTGACGGAGG TGCTGCTGAG 
GAAGAGGACG ATGATGAGCA CCAGGAATTC TGCCGCGTGT GCAAGGACGG TGGCGAGTTG 
CTGTGCTGCG ACTCATGTCC CTCCGCCTAT CACACATTCT GCTTGAATCC GCCTCTGGAC 
ACTATACCCG ATGGCGATTG GCGCTGTCCT CGTTGCAGCT GCCCTCCGCT CACTGGCAAG 
GCTGAGAAGA TCATCACCTG GCGATGGGCA CAGCGTAGCA ATGACGACGG TCCCTCCACC 
TCGAAGGGAT CAAAGAACAG TAACTCCCGG GTGCGCGAGT ACTTTATCAA GTGGCACAAC 
ATGTCCTACT GGCATTGCGA ATGGGTGCCC GAAGTGCAAC TGGACGTCCA TCATCCGCTC 
ATGATTCGTT CGTTCCAGCG CAAGTACGAC ATGGAAGAAC CGCCCAAGTT CGAGGAGTCG 
CTGGATGAGG CGGACACTCG TTACAAGCGT ATTCAGCGAC ACAAGGACAA GGTTGGCATG 
AAGGCAAACG ATGATGCCGA AGTGCTGGAG GAGCGGTTCT ACAAGAATGG CGTCAAGCCA 
GAGTGGCTCA TTGTTCAGCG CGTAATCAAC CATCGTACGG CAAGAGATGG CAGCACCATG 
TATCTGGTGA AGTGGCGCGA GTTGCCCTAT GACAAGTCCA CCTGGGAAGA AGAGGGTGAT 
GACATACAGG GCCTGCGGCA AGCCATCGAT TACTATCAGG ATCTGCGTGC GGTTTGCACC 
TCGGAGACAA CTCAGTCGCG CAGCAAAAAG AGCAAGAAGG GTCGCAAGTC CAAGCTCAAG 
GTCGAGGACG ACGAGGATCG CCCGGTCAAG CACTACACAC CGCCGCCGGA AAAGCCAACT 
ACTGATCTGA AGAAGAAGTA TGAGGATCAG CCGGCTTTCT TGGAGGGCAC TGGCATGCAA 
TTGCATCCCT ACCAGATCGA GGGCATTAAC TGGTTGCGCT ACAGCTGGGG CCAGGGCATC 
GACACCATCC TGGCCGACGA GATGGGTCTG GGTAAGACCA TTCAGACGGT CACCTTCCTG 
TACTCTTTGT ACAAGGAGGG CCATTGCCGC GGACCCTTCC TCGTGGCCGT GCCACTCTCG 
ACGCTAGTGA ACTGGGAGCG TGAGTTTGAG CTGTGGGCTC CGGACTTTTA CTGCATCACG 
TATATCGGTG ACAAGGACTC GAGAGCGGTT ATACGGGAGA ACGAGCTGAG CTTCGAGGAG 
GGCGCCATTC GTGGTAGCAA GGTTTCGCGT TTGCGGACCA CCCAGTATAA ATTCAACGTA 
CTGCTGACTA GCTACGAGCT GATCTCCATG GACGCTGCTT GCCTTGGCAG CATTGACTGG 
GCCGTGCTCG TGGTGGACGA GGCCCATCGC TTGAAGAGCA ACCAGAGCAA GTTCTTCCGT 
ATCCTGAACA GCTACACTAT TGCCTACAAG CTTCTGCTCA CCGGCACACC GCTGCAGAAC 
AATCTCGAGG AGCTGTTCCA TCTGCTCAAC TTCTTGAGCC GCGACAAGTT CAATGATCTG 
CAGGCCTTCC AGGGCGAATT CGCTGACGTT TCGAAGGAGG AGCAGGTTAA GCGTCTGCAC 
GAGATGCTTG GACCGCACAT GCTGCGTCGT TTGAAGACGG ATGTGCTGAA GAACATGCCG 
TCCAAGTCTG AGTTTATCGT GCGCGTCGAG CTGTCGGCCA TGCAGAAAAA GTTCTACAAG 
TTCATCTTGA CCAAGAACTA CGAGGCTTTG AATTCGAAGA GCGGTGGTGG CTCTTGCTCA 
CTGATCAACA TTATGATGGA CCTAAAGAAG TGCTGCAACC ATCCGTATCT CTTCCCCTCG 
GCCGCCGAGG AGGCAACCAC CGCTGCCGGT GGTCTCTACG AGATTAACTC GCTGACCAAG 
GCTGCTGGCA AGCTGGTCCT GCTATCCAAG ATGCTGAAGC AGCTGAAGGC ACAAAACCAT 
CGTGTCTTGA TTTTCTCCCA GATGACCAAG ATGTTGGACA TTCTCGAGGA CTTCCTCGAG 
GGCGAGCAGT ACAAGTACGA ACGTATTGAC GGTGGAATCA CAGGAACATT GCGTCAGGAA 
GCCATTGATA GGTTCAATGC ACCCGGAGCG CAGCAGTTTG TCTTCTTGCT GAGTACTCGA 
GCCGGAGGAT TGGGTATTAA TTTGGCCACG GCCGACACTG TCATTATTTA TGACTCCGAT 
TGGAATCCCC ACAACGATAT TCAGGCCTTC TCCCGAGCCC ATCGTATTGG CCAGGCTAAC 
AAGGTGATGA TCTATCGATT TGTTACTCGA AATTCTGTGG AGGAGCGCGT TACCCAGGTG 
GCCAAGCGTA AGATGATGTT GACTCATCTT GTGGTCCGTC CGGGAATGGG CGGTAAAGGC 
GCTAACTTTA CAAAGCAAGA ACTGGACGAT ATCCTTCGTT TCGGTACCGA GGATCTGTTC 
AAGGAGGATG ACAAGGAGGA AGCTATCCAC TATGACGACA AGGCGGTGGC CGAGCTGCTC 
GACAGAACCA ACCGCGGTAT CGAGGAGAAG GAGTCCTGGG CCAACGAGTA TCTGTCCTCG 
TTTAAGGTGG CTTCGTACGC CACTAAAGAA GAGGAGGAGG AGGAAGAGAC GGAGATTATT 
AAACAGGATG CCGAGAACTC TGATCCGGCG TACTGGGTGA AGCTGCTGCG GCATCACTAT 
GAACAGCACC AGGAAGATGT GGGTCGCAGC CTGGGCAAGG GCAAGCGCGT CCGCAAACAG 
GTCAACTACA CGGATGGCGG CGTTGTGGCG GCTGATACCA CGCGGGATGA TTCCAACTGG 
CAGGACAACG GCAGCGAGTA TAACTCCGAG TATTCAGCTG GCTCCGATGA GGATGGCGGT 
GACGATGACT TCGATGACCA GAATGGCGCA GAGAGAAAGG CCAAGCGGCG ATTGGAGCGT 
CGCGACGATC GTCCATTGCC TCCACTGTTG GCCCGCGTGG GTGGAAACAT CGAGGTGCTC 
GGTTTTAATG CCCGTCAACG CAAGAGCTTC CTCAATGCCA TTATGCGGTA CGGAATGCCG 
CCGCAAGATG CCTTCAACTC GCAATGGCTA GTCCGGGATT TGCGTGGTAA ATCGGAGCGC 
AATTTTAAGG CGTATGTTTC TCTGTTTATG CGGCATCTTT GCGAGCCGGG AGCGGACAAT 
GCGGAAACCT TTGCCGATGG TGTGCCGCGC GAAGGACTAT CCCGCCAGCA TGTGCTCACC 
CGGATCGGAG TGATGTCGCT AATTCGAAAG AAGGTACAGG AATTCGAGCA TATTAACGGA 
TACTACAGCA TGCCGGAGCT CATTCTCAAG CCGTGCGAGC CCGTACGATC AGCCCTGAAA 
CAGGATGTGG CGGCACTTGA AGCTCCGCCT ACCGGTGGCA ATGTCGACAA GAGCGCTACT 
ACTAGCAATA GCGTCACTCC AGCCACCAGT GCGGCTCCCA GTCCAGCGCC AGCCTCTGAA 
AAAGGTGAAG ACAAAGACAA GGACTCCGAA AAGGAGAAGG ATAAGACCTC GGCGGAGAAG 
AGCGAGGTGA AGCAAGAACA AGAGGCTGAA GAGGACAAGA AGCCTGGGGA TGTCAAGCAG 
GAGAACCCTG TGGAAGAAGC TGCCGGCGAC ACAAAGCCCA GCGACGCGGA GGTCAAAACC 
GAGGTGGCTA AAACAGAACC CAAGGAAGAG ACCAAGGACC CGGAGGTAAA GGAGGAGCCC 
AAGACCGAAG AGAAGGAAAA AGAAAAGGTG GATGACAAGA AACCAATACC ACCGACGACA 
GTAATTGACG ACGACGATGA CGATGTGATG ATTGTCAAGG AGGACGGTGA GCTAGAAAAG 
CCCAGTGCCA GTTCACCCAA GGATCAAAAG GCAGTCGCCG CTGCTACGTC TGCTGCAACT 
GGAGCCACTG GCAAGGGAGC GGAGGACAGC TTGGAGGTGC TAAAGCGCAA ATTTATGTTC 
AACATCGCCG ACGGCGGGTT CACCGAACTG CACACTTTGT GGCTCAACGA GGAGAAGGCT 
GCCGTTCCTG GGCGAGAATA CGAGATCTGG CACCGTCGCC ACGACTACTG GCTGTTGGCC 
GGAATTGTGA CCCATGGCTA TGGCCGTTGG CAGGATATAC AAAACGATAT CCGTTTCGCG 
ATCATAAACG AACCTTTCAA GATGGACGTT GGTAAGGGCA ACTTTCTGGA AATCAAAAAC 
AAGTTCCTGG CCCGGCGTTT TAAGTTGCTG GAGCAGGCGC TGGTCATCGA GGAGCAGCTG 
CGGCGAGCGG CCTACCTTAA CCTCGCCCAG GACCCCAGCC ACCCGGCAAT GTCGCTAAAC 
GCCCGTTTCG CAGAGGTCGA ATGCCTGGCT GAATCCCATC AGCATCTGAG CAAGGAATCG 
CTGGCCGGTA ACAAGCCCGC AAACGCTGTT CTGCACAAGG TGCTCAACCA GCTCGAAGAG 
CTGCTCTCGG ACATGAAGAG CGATGTGTCC CGGCTGCCAG CTACACTGGC TCGCATCCCA 
CCTGTTGCGC AACGGCTTCA GATGTCCGAG AGATCGATTC TCTCCCGACT GGCGGCCACC 
GCCGGAAATG CCTCCAATGC AGCTCAATTG ATGGCACAAT TCCCGGCTGG ATTCCAGGGC 
ACAACATTGC CAGCATTCAC GAGCGGACCG GCTGGCAACT TTGCCAACTT TCGGCCACAG 
TTCTCGGTGC CCGGCCAGCT ATCGAATAAT TCCGGCGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mi-2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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