Kap-alpha1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kap-alpha1
Entrez Gene ID	40160
Full Name	karyopherin alpha1
Synonyms	CG8548,Dalpha1,Dmalpha1,Dmel\CG8548,IMPalpha1,Imp-alpha1,Kap alpha1,Kapalpha1S1,Kpna1,alphaKap1,anon-WO0118547.276,imp alpha1,imp-alpha1,impalpha1
General protein information	Preferred Names karyopherin alpha1 Names CG8548 gene product from transcript CG8548-RA CG8548-PA Kap-alpha1-PA importin alpha1 importin-alpha1 karyopherin alpha 1 karyopherin-alpha1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 76C6-76C6

mRNA and Protein(s)

mRNA	Protein	Name
NM_079443.2	NP_524167.1	karyopherin alpha1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	KPNA6	XP_003307992.1
Canis lupus familiaris (dog)	KPNA6	XP_005617710.1
Gallus gallus (chicken)	KPNA6	NP_001012859.2
Arabidopsis thaliana (thale cress)	IMPA-2	NP_567485.4
Danio rerio (zebrafish)	LOC100149852	XP_001923921.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005401	XP_315411.4
Oryza sativa (rice)	Os05g0155500	NP_001054692.1
Bos taurus (cattle)	KPNA6	NP_001069422.1
Mus musculus (house mouse)	Kpna6	NP_032494.3
Drosophila melanogaster (fruit fly)	Kap-alpha1	NP_524167.1
Schizosaccharomyces pombe (fission yeast)	imp1	NP_596632.1
Homo sapiens (human)	KPNA6	NP_036448.1
	KPNA6	XP_001102290.1
Rattus norvegicus (Norway rat)	Kpna6	NP_001015029.2
Arabidopsis thaliana (thale cress)	IMPA-1	NP_187328.1
Oryza sativa (rice)	Os01g0253300	NP_001042611.1
Xenopus tropicalis (tropical clawed frog)	kpna6	NP_001008018.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study identified a potential nuclear localization signal at the N-terminal end of Yorkie as well as a critical role for Importin alpha 1 in Yorkie nuclear import.
Title: Importin ?1 Mediates Yorkie Nuclear Import via an N-terminal Non-canonical Nuclear Localization Signal.

This is supported by attenuated interaction of IMPa1 with TIM carrying a mutation previously shown to prevent nuclear entry of TIM and PER.
Title: Drosophila TIM binds importin ?1, and acts as an adapter to transport PER to the nucleus.

Drosophila alpha1 performs paralog-specific activities that are essential for gametogenesis and regulation of subcellular alpha1 localization may affect cell fate decisions.
Title: Drosophila importin alpha1 performs paralog-specific functions essential for gametogenesis.

each Imp-alpha protein carries during Drosophila spermatogenesis distinct, albeit overlapping, functions that may involve nuclear import of proteins, microtubule organisation, and other yet unknown processes
Title: Patterns of importin-alpha expression during Drosophila spermatogenesis.

The following Kap-alpha1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kap-alpha1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa19015	NM_079443.2 Latest version!	Drosophila melanogaster karyopherin alpha1 (Kap-alpha1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kap-alpha1 ( NM_079443.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524167.1 Drosophila melanogaster karyopherin alpha1 (Kap-alpha1), mRNA.

Price & Availability↑

CloneID	OFa19015
Clone ID Related Accession (Same CDS sequence)	NM_079443.2
Accession Version	NM_079443.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	karyopherin alpha1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079443.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGCCA GCCACAAACA GCGTTATAAA AATGCCGCCT TGGACTCCAC AGAGATGCGT 
CGTCGTCGCG AGGAGGTGGG CATCCAGTTG CGAAAAAACA AACGCGAACA GCAGCTCTTT 
AAGCGACGCA ATGTGGTTCT TGAGCCGGCT ACATCCTCAA CATCAGCCGG AGTGGAGAGC 
AACACCGATA ACGAACAGCA GGCTGCCGAC ATGCACATGG CTGACAGCAG CACTGGCGGT 
CAAAACGAGG AAGCTGCTGG AAGCGGGGCA CAGCCATCCG TAATCAACGA GGAAATGATT 
CAAATGCTCT TCAGTGGACG TGAAAACGAA CAGCTTGAGG CGACTCAAAA GTTCCGCAAA 
CTGCTGTCCC GCGATCCCAA TCCTCCCATT GAGGAAGTCA TCCAGAAGGG GATAGTGCCA 
CAGTTTGTCA CCTTTTTGCG AAATAGCGCA AATGCCACGC TGCAGTTCGA GGCCGCCTGG 
ACGCTAACGA ACATCGCATC GGGCACCTCG CAACAGACCA AGGTGGTTAT CGAGGCCGGG 
GCAGTGCCTA TCTTCATTGA TTTGCTCTCC AGTCCCCATG ATGATGTTCA GGAACAGGCC 
GTGTGGGCCC TAGGCAACAT AGCCGGTGAC TCACCCATGT GTCGAGACCA TTTGCTCGGC 
TCCGGCATTC TAGAGCCTCT CCTGCATGTA TTGAGCAACT CGGATCGCAT TACCATGATA 
CGGAATGCGG TGTGGACACT TTCAAACTTG TGCCGCGGCA AAAGCCCTCC CGCAGACTTT 
GCCAAAATTA GCCATGGTCT ACCAATACTG GCACGGTTGC TGAAGTACAC TGATGCGGAT 
GTACTAAGCG ATACTTGCTG GGCCATAGGC TACCTGTCAG ATGGTCCCAA TGATAAAATC 
CAGGCTGTAA TTGATGCGGG TGTTTGCCGT CGCCTGGTTG AGCTGCTATT ACATCCTCAG 
CAGAACGTGA GCACAGCTGC TCTGAGGGCC GTGGGCAATA TTGTGACAGG CGACGATCAG 
CAGACCCAGG TGATATTGGG GTACAACGCT TTGACCTGCA TTAGCCACCT GCTGCATTCG 
ACGGCGGAAA CAATCAAGAA GGAGTCGTGC TGGACAATCT CAAATATTGC AGCCGGCAAT 
CGTGAGCAGA TCCAGGCCTT AATCAATGCC AACATATTCC CGCAGCTGAT GGTTATCATG 
CAGACGGCCG AATTTAAGAC TCGCAAGGAG GCCGCATGGG CCATTACCAA CGCAACCAGC 
AGCGGTACGC ACGAACAGAT CCACTACCTG GTGCAGGTTG GCTGTGTGCC GCCCATGTGC 
GATTTTCTCA CCGTCGTCGA CTCGGACATC GTCCAAGTGG CGCTAAATGC ACTTGAAAAC 
ATTTTGAAGG CAGGTGAAAA GTTCCAGACG CGACCAAATC CCTATGCTAT TACCATCGAG 
GAATGCGGAG GTTTGGACAA AATAGAGTAT CTGCAGGCGC ATGAAAACCG CGACATCTAC 
CACAAATCAT TTTACATTAT CGAACAGTAC TTCGGCAACG AAGAGGAGGA TAGCCGGGTG 
GCTCCCGTGG CAGGCACGCA ACAGTTTGAG TTCGATCCGG ACAACATGCC CAGCACCGGA 
TTTAACTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524167.1
CDS	113..1744
Translation	MSASHKQRYKNAALDSTEMRRRREEVGIQLRKNKREQQLFKRRNVVLEPATSSTSAGVESNTDNEQQAADMHMADSSTGGQNEEAAGSGAQPSVINEEMIQMLFSGRENEQLEATQKFRKLLSRDPNPPIEEVIQKGIVPQFVTFLRNSANATLQFEAAWTLTNIASGTSQQTKVVIEAGAVPIFIDLLSSPHDDVQEQAVWALGNIAGDSPMCRDHLLGSGILEPLLHVLSNSDRITMIRNAVWTLSNLCRGKSPPADFAKISHGLPILARLLKYTDADVLSDTCWAIGYLSDGPNDKIQAVIDAGVCRRLVELLLHPQQNVSTAALRAVGNIVTGDDQQTQVILGYNALTCISHLLHSTAETIKKESCWTISNIAAGNREQIQALINANIFPQLMVIMQTAEFKTRKEAAWAITNATSSGTHEQIHYLVQVGCVPPMCDFLTVVDSDIVQVALNALENILKAGEKFQTRPNPYAITIEECGGLDKIEYLQAHENRDIYHKSFYIIEQYFGNEEEDSRVAPVAGTQQFEFDPDNMPSTGFNF

Target ORF information:

RefSeq Version	NM_079443.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster karyopherin alpha1 (Kap-alpha1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079443.2

ORF Insert Sequence:

ATGTCTGCCA GCCACAAACA GCGTTATAAA AATGCCGCCT TGGACTCCAC AGAGATGCGT 
CGTCGTCGCG AGGAGGTGGG CATCCAGTTG CGAAAAAACA AACGCGAACA GCAGCTCTTT 
AAGCGACGCA ATGTGGTTCT TGAGCCGGCT ACATCCTCAA CATCAGCCGG AGTGGAGAGC 
AACACCGATA ACGAACAGCA GGCTGCCGAC ATGCACATGG CTGACAGCAG CACTGGCGGT 
CAAAACGAGG AAGCTGCTGG AAGCGGGGCA CAGCCATCCG TAATCAACGA GGAAATGATT 
CAAATGCTCT TCAGTGGACG TGAAAACGAA CAGCTTGAGG CGACTCAAAA GTTCCGCAAA 
CTGCTGTCCC GCGATCCCAA TCCTCCCATT GAGGAAGTCA TCCAGAAGGG GATAGTGCCA 
CAGTTTGTCA CCTTTTTGCG AAATAGCGCA AATGCCACGC TGCAGTTCGA GGCCGCCTGG 
ACGCTAACGA ACATCGCATC GGGCACCTCG CAACAGACCA AGGTGGTTAT CGAGGCCGGG 
GCAGTGCCTA TCTTCATTGA TTTGCTCTCC AGTCCCCATG ATGATGTTCA GGAACAGGCC 
GTGTGGGCCC TAGGCAACAT AGCCGGTGAC TCACCCATGT GTCGAGACCA TTTGCTCGGC 
TCCGGCATTC TAGAGCCTCT CCTGCATGTA TTGAGCAACT CGGATCGCAT TACCATGATA 
CGGAATGCGG TGTGGACACT TTCAAACTTG TGCCGCGGCA AAAGCCCTCC CGCAGACTTT 
GCCAAAATTA GCCATGGTCT ACCAATACTG GCACGGTTGC TGAAGTACAC TGATGCGGAT 
GTACTAAGCG ATACTTGCTG GGCCATAGGC TACCTGTCAG ATGGTCCCAA TGATAAAATC 
CAGGCTGTAA TTGATGCGGG TGTTTGCCGT CGCCTGGTTG AGCTGCTATT ACATCCTCAG 
CAGAACGTGA GCACAGCTGC TCTGAGGGCC GTGGGCAATA TTGTGACAGG CGACGATCAG 
CAGACCCAGG TGATATTGGG GTACAACGCT TTGACCTGCA TTAGCCACCT GCTGCATTCG 
ACGGCGGAAA CAATCAAGAA GGAGTCGTGC TGGACAATCT CAAATATTGC AGCCGGCAAT 
CGTGAGCAGA TCCAGGCCTT AATCAATGCC AACATATTCC CGCAGCTGAT GGTTATCATG 
CAGACGGCCG AATTTAAGAC TCGCAAGGAG GCCGCATGGG CCATTACCAA CGCAACCAGC 
AGCGGTACGC ACGAACAGAT CCACTACCTG GTGCAGGTTG GCTGTGTGCC GCCCATGTGC 
GATTTTCTCA CCGTCGTCGA CTCGGACATC GTCCAAGTGG CGCTAAATGC ACTTGAAAAC 
ATTTTGAAGG CAGGTGAAAA GTTCCAGACG CGACCAAATC CCTATGCTAT TACCATCGAG 
GAATGCGGAG GTTTGGACAA AATAGAGTAT CTGCAGGCGC ATGAAAACCG CGACATCTAC 
CACAAATCAT TTTACATTAT CGAACAGTAC TTCGGCAACG AAGAGGAGGA TAGCCGGGTG 
GCTCCCGTGG CAGGCACGCA ACAGTTTGAG TTCGATCCGG ACAACATGCC CAGCACCGGA 
TTTAACTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Kap-alpha1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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