Lon cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lon
Entrez Gene ID	40138
Full Name	Lon protease
Synonyms	CG8798,Dmel\CG8798,anon-WO0118547.350
General protein information	Preferred Names Lon protease Names CG8798 gene product from transcript CG8798-RA CG8798-PA CG8798-PC Lon-PA Lon-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 76B9-76B9

mRNA and Protein(s)

mRNA	Protein	Name
NM_140876.3	NP_649133.1	Lon protease, isoform A
NM_168806.3	NP_730435.2	Lon protease, isoform C

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LONP1	XP_001143873.1
Canis lupus familiaris (dog)	LONP1	XP_854391.2
Mus musculus (house mouse)	Lonp1	NP_083058.2
Rattus norvegicus (Norway rat)	Lonp1	NP_596895.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010451	XP_311497.4
Caenorhabditis elegans (roundworm)	C34B2.6	NP_492796.1
Homo sapiens (human)	LONP1	NP_004784.2
Bos taurus (cattle)	LONP1	NP_001015569.2
Danio rerio (zebrafish)	lonp1	XP_691712.4
Saccharomyces cerevisiae (baker's yeast)	PIM1	NP_009531.1
Kluyveromyces lactis	KLLA0E10407g	XP_454420.1
Magnaporthe oryzae (rice blast fungus)	MGG_11141	XP_003714792.1
Xenopus tropicalis (tropical clawed frog)	LOC100492505	XP_002938599.1
	LONP1	XP_001088663.2
Gallus gallus (chicken)	LONP1	XP_001232112.2
Oryza sativa (rice)	Os07g0689300	NP_001175344.1
Schizosaccharomyces pombe (fission yeast)	lon1	NP_593035.1
Drosophila melanogaster (fruit fly)	Lon	NP_649133.1
Neurospora crassa	NCU05261	XP_961826.1
Arabidopsis thaliana (thale cress)	LON1	NP_568490.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Lon regulates mitochondrial transcription by stabilizing the mitochondrial TFAMmtDNA ratio via selective degradation of TFAM.
Title: Mitochondrial Lon protease regulates mitochondrial DNA copy number and transcription by selective degradation of mitochondrial transcription factor A (TFAM).

The following Lon gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lon cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18988	NM_140876.3 Latest version!	Drosophila melanogaster Lon protease (Lon), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa18989	NM_168806.3 Latest version!	Drosophila melanogaster Lon protease (Lon), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lon ( NM_140876.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649133.1 Drosophila melanogaster Lon protease (Lon), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18988
Clone ID Related Accession (Same CDS sequence)	NM_140876.3
Accession Version	NM_140876.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Lon protease, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140876.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTAGCCC GCGCTATCCG AGTGCGCCCC ATGATGCGTG GCATCGCCTC GTCGTCAGTG 
TGGAACCGGA ATCGTCCCAT TCAGAGTTCC CTGATGCAAT ACTGCCGGGA TCGGTCGTTG 
CGCCTCCAGC GGCTCCACGG AGCCAATTTG ATGGTGCAGC GCTTCTACAG CCGCAAGCGG 
GATGATTCCA ACGGGGATAT TATTATGGGA CCCGATCTTA TGTCCGATCA AGATACCCAT 
CTTCCGGCAA CTGTGGCGGT GCCGGACGTG TGGCCACATG TTCCGTTGTT GGCCATGCGC 
AAGAATCCTC TCTTTCCCCG CTTTATGAAG ATAGTGGAGG TATCCAATCC CATCATCATG 
GATCTACTCC GTCGCAAAGT CAAGCTGAAT CAACCTTACG TAGGCGTCTT CCTTAAGAAA 
ACTGATGGCG AAGAGGAGCT TATCACCAAT TTGAATGATG TCTACAATCT CGGAACATTT 
GCACAAATCC AAGAGCTGCA GGATCTCGGT GATAAGCTGC GCATGGTTGT GGTGGCCCAT 
CGTCGTATTC GTATAACAGG TCAGGTGGTA GAGGACGTGC CACCTCCTAA GCCCGCTGAA 
GATCAAAGCA CTGATCAAGC GGATGCGGCA CCTATAAAAT CAAGATCCGA CCCAGCCCGA 
AAGCCCCGTG GCCGAATTCC CAGGAGTCGG ACCGGAAAAT CACGTGAATC GGCGGCTGCT 
GAAGAACTTA TACAAAACCA GACTCTGGAG CCGCCACTGA AATCAGGCAA AGTCGAGTCA 
TCGAGCTTGC CAAAGCCTCC AACAGAAGAA AAGATAGTGG AACCTGAGAC TGGTGCCAAA 
GAAAATGTTA ATCAATCTGC GCCCAGCGCC CAACCTGTTC TTATTGTTGA AGTGGAGAAC 
GTAAAGCAAC CGATCTATAA ACAAACGGAA GAGGTGAAGG CATTAACTCA GGAGATAATC 
AAGACTCTTC GGGACATAAT CACAATGAAT CCCTTGTATA GAGAGAGTCT ACAGCAGATG 
CTGCACCAAA ATCAACGAGT TGTCGACAAC CCCATTTATC TGTGCGATTT AGGAGCATCC 
CTTTCCGCTG GAGAACCAGC TGAGCTGCAA AAAATTCTTG AGGAAACAGA TATCCCAGAA 
CGTCTACAAT TGGCCTTAAC TTTACTCAAA AAGGAGTTGG AACTCTCCAG GCTCCAACAA 
AAAATTGGAC GCGAAGTAGA GGAAAAAGTC AAGCAGCAAC ATCGCAAATA TATACTCCAG 
GAACAGTTGA AAGTCATCAA AAAAGAACTG GGCATCGAAA AAGACGACAA GGATGCCATT 
GGTGAAAAGT ACCGCGAAAA GCTGAAGGAT AAGGTGGTTC CCGAAGCTAT TATGACGGTT 
ATTGATGAAG AACTCACCAA GCTGAACTTC CTGGAGAGTC ATAGTTCCGA GTTTAATGTA 
ACTCGCAATT ACCTCGACTG GCTTACTTCC CTACCTTGGG GCGTTATCAG CACCGAAAAT 
CTCTGCCTGG AGAAGGCCAC TGAAACACTA AACGACGATC ACTACGGAAT GGAGGATATC 
AAGAAGCGTA TTTTAGAATT TATTGCCGTC AGTTCCCTCA AGGGCTCTAC GCAGGGCAAG 
ATCTTGTGCT TTCATGGACC CCCTGGTGTG GGCAAGACAA GCATAGCAAA GTCCATTGCA 
CGCGCTTTGA ATCGCGAGTA CTTCCGCTTT AGTGTGGGCG GCATGACAGA CGTTGCTGAG 
ATCAAGGGAC ATCGCCGCAC CTATGTGGGC GCAATGCCGG GTAAACTGAT TCAGTGCCTG 
AAGAAGACTA AGATTGAAAA TCCTCTGGTA CTCATCGACG AGGTGGACAA GATCGGCAAG 
GGCTACCAGG GAGATCCTAG CTCAGCATTG TTAGAGCTAC TAGATCCCGA GCAGAATGCG 
AATTTCCTGG ACCACTACCT CGATGTGCCG GTAGACCTCT CGCGAGTGCT CTTCATCTGC 
ACAGCTAATG TGATCGACAC TATTCCCGAG CCTTTAAGAG ATCGCATGGA ATTGATTGAG 
ATGTCTGGGT ATGTTGCCGA AGAGAAGATC GCCATCGCTC GTCAGTATCT GATGCCACAA 
GCGATGAAGG ACTGTGGATT AACGGACAAG CATATCAACA TTTCCGAGGA TGCCCTAAAC 
ATGCTGATTC GCAGTTATTG CCGGGAATCC GGGGTGCGAA ACCTGCAAAA GCATATCGAG 
AAAGTTATTC GCAAGGTGGC CTTCCGAGTG GTCAAAAAAG AAGGCGAGCA CTTTCCGGTT 
AACGCTGATA ATCTGACCAC ATTCCTGGGC AAACAAATCT TTAGCTCAGA TCGCATGTAC 
GCAACCACAC CGGTGGGTGT AGTCATGGGT CTGGCCTGGA CCGCCATGGG TGGTTCATCG 
CTTTACATCG AAACCTCAAG GCGTCACATT CGCCAGGGGG CAAAAACAGA TCCGAACACT 
GTCGCCGGAT CGCTGCACAT CACCGGAAAT TTGGGAGATG TCATGAAGGA GTCGGCCCAA 
ATAGCCTTAA CGGTGGCGCG CAACTTTTTA TATTCGCTGG AACCCAATAA TCTGTTCTTA 
GAGCAAGAGC ACATTCACCT CCATGTTCCT GAAGGTGCCA CACCGAAAGA TGGCCCCAGT 
GCGGGCATCA CCATTATTAC AGCTCTCGTA TCGTTGGCCA CTGGAAAGCC GGTGCGCCAA 
GATATCGCTA TGACGGGCGA AGTATCCCTG AAGGGAAAAG TGCTACCCGT GGGTGGTATT 
AAGGAAAAGA CGATTGCAGC ACGTCGTAGT GGAGTGAATT GTCTTATTCT GCCTGTGGAT 
AACAAGAAAG ACTTCGAGGA GTTGCCCACG TACATTACAG ATGGTCTGGA GGTTCACTTT 
GCCACCACCT ACGAGGATGT CTACAAGATA GCGTTTACTG ATGTAACCGA AACGACAACC 
AACAATGTTG AAGAGCAGGA GCCGCTGCAG AAGCTTTCCA GTGCGGCCGC TGCCAAATCG 
GAGACGTGGC CTTATTCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649133.1
CDS	118..3138
Translation	MLARAIRVRPMMRGIASSSVWNRNRPIQSSLMQYCRDRSLRLQRLHGANLMVQRFYSRKRDDSNGDIIMGPDLMSDQDTHLPATVAVPDVWPHVPLLAMRKNPLFPRFMKIVEVSNPIIMDLLRRKVKLNQPYVGVFLKKTDGEEELITNLNDVYNLGTFAQIQELQDLGDKLRMVVVAHRRIRITGQVVEDVPPPKPAEDQSTDQADAAPIKSRSDPARKPRGRIPRSRTGKSRESAAAEELIQNQTLEPPLKSGKVESSSLPKPPTEEKIVEPETGAKENVNQSAPSAQPVLIVEVENVKQPIYKQTEEVKALTQEIIKTLRDIITMNPLYRESLQQMLHQNQRVVDNPIYLCDLGASLSAGEPAELQKILEETDIPERLQLALTLLKKELELSRLQQKIGREVEEKVKQQHRKYILQEQLKVIKKELGIEKDDKDAIGEKYREKLKDKVVPEAIMTVIDEELTKLNFLESHSSEFNVTRNYLDWLTSLPWGVISTENLCLEKATETLNDDHYGMEDIKKRILEFIAVSSLKGSTQGKILCFHGPPGVGKTSIAKSIARALNREYFRFSVGGMTDVAEIKGHRRTYVGAMPGKLIQCLKKTKIENPLVLIDEVDKIGKGYQGDPSSALLELLDPEQNANFLDHYLDVPVDLSRVLFICTANVIDTIPEPLRDRMELIEMSGYVAEEKIAIARQYLMPQAMKDCGLTDKHINISEDALNMLIRSYCRESGVRNLQKHIEKVIRKVAFRVVKKEGEHFPVNADNLTTFLGKQIFSSDRMYATTPVGVVMGLAWTAMGGSSLYIETSRRHIRQGAKTDPNTVAGSLHITGNLGDVMKESAQIALTVARNFLYSLEPNNLFLEQEHIHLHVPEGATPKDGPSAGITIITALVSLATGKPVRQDIAMTGEVSLKGKVLPVGGIKEKTIAARRSGVNCLILPVDNKKDFEELPTYITDGLEVHFATTYEDVYKIAFTDVTETTTNNVEEQEPLQKLSSAAAAKSETWPYS

Target ORF information:

RefSeq Version	NM_140876.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Lon protease (Lon), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140876.3

ORF Insert Sequence:

ATGTTAGCCC GCGCTATCCG AGTGCGCCCC ATGATGCGTG GCATCGCCTC GTCGTCAGTG 
TGGAACCGGA ATCGTCCCAT TCAGAGTTCC CTGATGCAAT ACTGCCGGGA TCGGTCGTTG 
CGCCTCCAGC GGCTCCACGG AGCCAATTTG ATGGTGCAGC GCTTCTACAG CCGCAAGCGG 
GATGATTCCA ACGGGGATAT TATTATGGGA CCCGATCTTA TGTCCGATCA AGATACCCAT 
CTTCCGGCAA CTGTGGCGGT GCCGGACGTG TGGCCACATG TTCCGTTGTT GGCCATGCGC 
AAGAATCCTC TCTTTCCCCG CTTTATGAAG ATAGTGGAGG TATCCAATCC CATCATCATG 
GATCTACTCC GTCGCAAAGT CAAGCTGAAT CAACCTTACG TAGGCGTCTT CCTTAAGAAA 
ACTGATGGCG AAGAGGAGCT TATCACCAAT TTGAATGATG TCTACAATCT CGGAACATTT 
GCACAAATCC AAGAGCTGCA GGATCTCGGT GATAAGCTGC GCATGGTTGT GGTGGCCCAT 
CGTCGTATTC GTATAACAGG TCAGGTGGTA GAGGACGTGC CACCTCCTAA GCCCGCTGAA 
GATCAAAGCA CTGATCAAGC GGATGCGGCA CCTATAAAAT CAAGATCCGA CCCAGCCCGA 
AAGCCCCGTG GCCGAATTCC CAGGAGTCGG ACCGGAAAAT CACGTGAATC GGCGGCTGCT 
GAAGAACTTA TACAAAACCA GACTCTGGAG CCGCCACTGA AATCAGGCAA AGTCGAGTCA 
TCGAGCTTGC CAAAGCCTCC AACAGAAGAA AAGATAGTGG AACCTGAGAC TGGTGCCAAA 
GAAAATGTTA ATCAATCTGC GCCCAGCGCC CAACCTGTTC TTATTGTTGA AGTGGAGAAC 
GTAAAGCAAC CGATCTATAA ACAAACGGAA GAGGTGAAGG CATTAACTCA GGAGATAATC 
AAGACTCTTC GGGACATAAT CACAATGAAT CCCTTGTATA GAGAGAGTCT ACAGCAGATG 
CTGCACCAAA ATCAACGAGT TGTCGACAAC CCCATTTATC TGTGCGATTT AGGAGCATCC 
CTTTCCGCTG GAGAACCAGC TGAGCTGCAA AAAATTCTTG AGGAAACAGA TATCCCAGAA 
CGTCTACAAT TGGCCTTAAC TTTACTCAAA AAGGAGTTGG AACTCTCCAG GCTCCAACAA 
AAAATTGGAC GCGAAGTAGA GGAAAAAGTC AAGCAGCAAC ATCGCAAATA TATACTCCAG 
GAACAGTTGA AAGTCATCAA AAAAGAACTG GGCATCGAAA AAGACGACAA GGATGCCATT 
GGTGAAAAGT ACCGCGAAAA GCTGAAGGAT AAGGTGGTTC CCGAAGCTAT TATGACGGTT 
ATTGATGAAG AACTCACCAA GCTGAACTTC CTGGAGAGTC ATAGTTCCGA GTTTAATGTA 
ACTCGCAATT ACCTCGACTG GCTTACTTCC CTACCTTGGG GCGTTATCAG CACCGAAAAT 
CTCTGCCTGG AGAAGGCCAC TGAAACACTA AACGACGATC ACTACGGAAT GGAGGATATC 
AAGAAGCGTA TTTTAGAATT TATTGCCGTC AGTTCCCTCA AGGGCTCTAC GCAGGGCAAG 
ATCTTGTGCT TTCATGGACC CCCTGGTGTG GGCAAGACAA GCATAGCAAA GTCCATTGCA 
CGCGCTTTGA ATCGCGAGTA CTTCCGCTTT AGTGTGGGCG GCATGACAGA CGTTGCTGAG 
ATCAAGGGAC ATCGCCGCAC CTATGTGGGC GCAATGCCGG GTAAACTGAT TCAGTGCCTG 
AAGAAGACTA AGATTGAAAA TCCTCTGGTA CTCATCGACG AGGTGGACAA GATCGGCAAG 
GGCTACCAGG GAGATCCTAG CTCAGCATTG TTAGAGCTAC TAGATCCCGA GCAGAATGCG 
AATTTCCTGG ACCACTACCT CGATGTGCCG GTAGACCTCT CGCGAGTGCT CTTCATCTGC 
ACAGCTAATG TGATCGACAC TATTCCCGAG CCTTTAAGAG ATCGCATGGA ATTGATTGAG 
ATGTCTGGGT ATGTTGCCGA AGAGAAGATC GCCATCGCTC GTCAGTATCT GATGCCACAA 
GCGATGAAGG ACTGTGGATT AACGGACAAG CATATCAACA TTTCCGAGGA TGCCCTAAAC 
ATGCTGATTC GCAGTTATTG CCGGGAATCC GGGGTGCGAA ACCTGCAAAA GCATATCGAG 
AAAGTTATTC GCAAGGTGGC CTTCCGAGTG GTCAAAAAAG AAGGCGAGCA CTTTCCGGTT 
AACGCTGATA ATCTGACCAC ATTCCTGGGC AAACAAATCT TTAGCTCAGA TCGCATGTAC 
GCAACCACAC CGGTGGGTGT AGTCATGGGT CTGGCCTGGA CCGCCATGGG TGGTTCATCG 
CTTTACATCG AAACCTCAAG GCGTCACATT CGCCAGGGGG CAAAAACAGA TCCGAACACT 
GTCGCCGGAT CGCTGCACAT CACCGGAAAT TTGGGAGATG TCATGAAGGA GTCGGCCCAA 
ATAGCCTTAA CGGTGGCGCG CAACTTTTTA TATTCGCTGG AACCCAATAA TCTGTTCTTA 
GAGCAAGAGC ACATTCACCT CCATGTTCCT GAAGGTGCCA CACCGAAAGA TGGCCCCAGT 
GCGGGCATCA CCATTATTAC AGCTCTCGTA TCGTTGGCCA CTGGAAAGCC GGTGCGCCAA 
GATATCGCTA TGACGGGCGA AGTATCCCTG AAGGGAAAAG TGCTACCCGT GGGTGGTATT 
AAGGAAAAGA CGATTGCAGC ACGTCGTAGT GGAGTGAATT GTCTTATTCT GCCTGTGGAT 
AACAAGAAAG ACTTCGAGGA GTTGCCCACG TACATTACAG ATGGTCTGGA GGTTCACTTT 
GCCACCACCT ACGAGGATGT CTACAAGATA GCGTTTACTG ATGTAACCGA AACGACAACC 
AACAATGTTG AAGAGCAGGA GCCGCTGCAG AAGCTTTCCA GTGCGGCCGC TGCCAAATCG 
GAGACGTGGC CTTATTCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lon ( NM_168806.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730435.2 Drosophila melanogaster Lon protease (Lon), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa18989
Clone ID Related Accession (Same CDS sequence)	NM_168806.3
Accession Version	NM_168806.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Lon protease, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168806.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTAGCCC GCGCTATCCG AGTGCGCCCC ATGATGCGTG GCATCGCCTC GTCGTCAGTG 
TGGAACCGGA ATCGTCCCAT TCAGAGTTCC CTGATGCAAT ACTGCCGGGA TCGGTCGTTG 
CGCCTCCAGC GGCTCCACGG AGCCAATTTG ATGGTGCAGC GCTTCTACAG CCGCAAGCGG 
GATGATTCCA ACGGGGATAT TATTATGGGA CCCGATCTTA TGTCCGATCA AGATACCCAT 
CTTCCGGCAA CTGTGGCGGT GCCGGACGTG TGGCCACATG TTCCGTTGTT GGCCATGCGC 
AAGAATCCTC TCTTTCCCCG CTTTATGAAG ATAGTGGAGG TATCCAATCC CATCATCATG 
GATCTACTCC GTCGCAAAGT CAAGCTGAAT CAACCTTACG TAGGCGTCTT CCTTAAGAAA 
ACTGATGGCG AAGAGGAGCT TATCACCAAT TTGAATGATG TCTACAATCT CGGAACATTT 
GCACAAATCC AAGAGCTGCA GGATCTCGGT GATAAGCTGC GCATGGTTGT GGTGGCCCAT 
CGTCGTATTC GTATAACAGG TCAGGTGGTA GAGGACGTGC CACCTCCTAA GCCCGTGAAA 
ATGACAACTT TGCATTATCC TCTATTTAAT ATAAAATTAC AAATCCCAGC TGAAGATCAA 
AGCACTGATC AAGCGGATGC GGCACCTATA AAATCAAGAT CCGACCCAGC CCGAAAGCCC 
CGTGGCCGAA TTCCCAGGAG TCGGACCGGA AAATCACGTG AATCGGCGGC TGCTGAAGAA 
CTTATACAAA ACCAGACTCT GGAGCCGCCA CTGAAATCAG GCAAAGTCGA GTCATCGAGC 
TTGCCAAAGC CTCCAACAGA AGAAAAGATA GTGGAACCTG AGACTGGTGC CAAAGAAAAT 
GTTAATCAAT CTGCGCCCAG CGCCCAACCT GTTCTTATTG TTGAAGTGGA GAACGTAAAG 
CAACCGATCT ATAAACAAAC GGAAGAGGTG AAGGCATTAA CTCAGGAGAT AATCAAGACT 
CTTCGGGACA TAATCACAAT GAATCCCTTG TATAGAGAGA GTCTACAGCA GATGCTGCAC 
CAAAATCAAC GAGTTGTCGA CAACCCCATT TATCTGTGCG ATTTAGGAGC ATCCCTTTCC 
GCTGGAGAAC CAGCTGAGCT GCAAAAAATT CTTGAGGAAA CAGATATCCC AGAACGTCTA 
CAATTGGCCT TAACTTTACT CAAAAAGGAG TTGGAACTCT CCAGGCTCCA ACAAAAAATT 
GGACGCGAAG TAGAGGAAAA AGTCAAGCAG CAACATCGCA AATATATACT CCAGGAACAG 
TTGAAAGTCA TCAAAAAAGA ACTGGGCATC GAAAAAGACG ACAAGGATGC CATTGGTGAA 
AAGTACCGCG AAAAGCTGAA GGATAAGGTG GTTCCCGAAG CTATTATGAC GGTTATTGAT 
GAAGAACTCA CCAAGCTGAA CTTCCTGGAG AGTCATAGTT CCGAGTTTAA TGTAACTCGC 
AATTACCTCG ACTGGCTTAC TTCCCTACCT TGGGGCGTTA TCAGCACCGA AAATCTCTGC 
CTGGAGAAGG CCACTGAAAC ACTAAACGAC GATCACTACG GAATGGAGGA TATCAAGAAG 
CGTATTTTAG AATTTATTGC CGTCAGTTCC CTCAAGGGCT CTACGCAGGG CAAGATCTTG 
TGCTTTCATG GACCCCCTGG TGTGGGCAAG ACAAGCATAG CAAAGTCCAT TGCACGCGCT 
TTGAATCGCG AGTACTTCCG CTTTAGTGTG GGCGGCATGA CAGACGTTGC TGAGATCAAG 
GGACATCGCC GCACCTATGT GGGCGCAATG CCGGGTAAAC TGATTCAGTG CCTGAAGAAG 
ACTAAGATTG AAAATCCTCT GGTACTCATC GACGAGGTGG ACAAGATCGG CAAGGGCTAC 
CAGGGAGATC CTAGCTCAGC ATTGTTAGAG CTACTAGATC CCGAGCAGAA TGCGAATTTC 
CTGGACCACT ACCTCGATGT GCCGGTAGAC CTCTCGCGAG TGCTCTTCAT CTGCACAGCT 
AATGTGATCG ACACTATTCC CGAGCCTTTA AGAGATCGCA TGGAATTGAT TGAGATGTCT 
GGGTATGTTG CCGAAGAGAA GATCGCCATC GCTCGTCAGT ATCTGATGCC ACAAGCGATG 
AAGGACTGTG GATTAACGGA CAAGCATATC AACATTTCCG AGGATGCCCT AAACATGCTG 
ATTCGCAGTT ATTGCCGGGA ATCCGGGGTG CGAAACCTGC AAAAGCATAT CGAGAAAGTT 
ATTCGCAAGG TGGCCTTCCG AGTGGTCAAA AAAGAAGGCG AGCACTTTCC GGTTAACGCT 
GATAATCTGA CCACATTCCT GGGCAAACAA ATCTTTAGCT CAGATCGCAT GTACGCAACC 
ACACCGGTGG GTGTAGTCAT GGGTCTGGCC TGGACCGCCA TGGGTGGTTC ATCGCTTTAC 
ATCGAAACCT CAAGGCGTCA CATTCGCCAG GGGGCAAAAA CAGATCCGAA CACTGTCGCC 
GGATCGCTGC ACATCACCGG AAATTTGGGA GATGTCATGA AGGAGTCGGC CCAAATAGCC 
TTAACGGTGG CGCGCAACTT TTTATATTCG CTGGAACCCA ATAATCTGTT CTTAGAGCAA 
GAGCACATTC ACCTCCATGT TCCTGAAGGT GCCACACCGA AAGATGGCCC CAGTGCGGGC 
ATCACCATTA TTACAGCTCT CGTATCGTTG GCCACTGGAA AGCCGGTGCG CCAAGATATC 
GCTATGACGG GCGAAGTATC CCTGAAGGGA AAAGTGCTAC CCGTGGGTGG TATTAAGGAA 
AAGACGATTG CAGCACGTCG TAGTGGAGTG AATTGTCTTA TTCTGCCTGT GGATAACAAG 
AAAGACTTCG AGGAGTTGCC CACGTACATT ACAGATGGTC TGGAGGTTCA CTTTGCCACC 
ACCTACGAGG ATGTCTACAA GATAGCGTTT ACTGATGTAA CCGAAACGAC AACCAACAAT 
GTTGAAGAGC AGGAGCCGCT GCAGAAGCTT TCCAGTGCGG CCGCTGCCAA ATCGGAGACG 
TGGCCTTATT CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730435.2
CDS	118..3192
Translation	MLARAIRVRPMMRGIASSSVWNRNRPIQSSLMQYCRDRSLRLQRLHGANLMVQRFYSRKRDDSNGDIIMGPDLMSDQDTHLPATVAVPDVWPHVPLLAMRKNPLFPRFMKIVEVSNPIIMDLLRRKVKLNQPYVGVFLKKTDGEEELITNLNDVYNLGTFAQIQELQDLGDKLRMVVVAHRRIRITGQVVEDVPPPKPVKMTTLHYPLFNIKLQIPAEDQSTDQADAAPIKSRSDPARKPRGRIPRSRTGKSRESAAAEELIQNQTLEPPLKSGKVESSSLPKPPTEEKIVEPETGAKENVNQSAPSAQPVLIVEVENVKQPIYKQTEEVKALTQEIIKTLRDIITMNPLYRESLQQMLHQNQRVVDNPIYLCDLGASLSAGEPAELQKILEETDIPERLQLALTLLKKELELSRLQQKIGREVEEKVKQQHRKYILQEQLKVIKKELGIEKDDKDAIGEKYREKLKDKVVPEAIMTVIDEELTKLNFLESHSSEFNVTRNYLDWLTSLPWGVISTENLCLEKATETLNDDHYGMEDIKKRILEFIAVSSLKGSTQGKILCFHGPPGVGKTSIAKSIARALNREYFRFSVGGMTDVAEIKGHRRTYVGAMPGKLIQCLKKTKIENPLVLIDEVDKIGKGYQGDPSSALLELLDPEQNANFLDHYLDVPVDLSRVLFICTANVIDTIPEPLRDRMELIEMSGYVAEEKIAIARQYLMPQAMKDCGLTDKHINISEDALNMLIRSYCRESGVRNLQKHIEKVIRKVAFRVVKKEGEHFPVNADNLTTFLGKQIFSSDRMYATTPVGVVMGLAWTAMGGSSLYIETSRRHIRQGAKTDPNTVAGSLHITGNLGDVMKESAQIALTVARNFLYSLEPNNLFLEQEHIHLHVPEGATPKDGPSAGITIITALVSLATGKPVRQDIAMTGEVSLKGKVLPVGGIKEKTIAARRSGVNCLILPVDNKKDFEELPTYITDGLEVHFATTYEDVYKIAFTDVTETTTNNVEEQEPLQKLSSAAAAKSETWPYS

Target ORF information:

RefSeq Version	NM_168806.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Lon protease (Lon), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168806.3

ORF Insert Sequence:

ATGTTAGCCC GCGCTATCCG AGTGCGCCCC ATGATGCGTG GCATCGCCTC GTCGTCAGTG 
TGGAACCGGA ATCGTCCCAT TCAGAGTTCC CTGATGCAAT ACTGCCGGGA TCGGTCGTTG 
CGCCTCCAGC GGCTCCACGG AGCCAATTTG ATGGTGCAGC GCTTCTACAG CCGCAAGCGG 
GATGATTCCA ACGGGGATAT TATTATGGGA CCCGATCTTA TGTCCGATCA AGATACCCAT 
CTTCCGGCAA CTGTGGCGGT GCCGGACGTG TGGCCACATG TTCCGTTGTT GGCCATGCGC 
AAGAATCCTC TCTTTCCCCG CTTTATGAAG ATAGTGGAGG TATCCAATCC CATCATCATG 
GATCTACTCC GTCGCAAAGT CAAGCTGAAT CAACCTTACG TAGGCGTCTT CCTTAAGAAA 
ACTGATGGCG AAGAGGAGCT TATCACCAAT TTGAATGATG TCTACAATCT CGGAACATTT 
GCACAAATCC AAGAGCTGCA GGATCTCGGT GATAAGCTGC GCATGGTTGT GGTGGCCCAT 
CGTCGTATTC GTATAACAGG TCAGGTGGTA GAGGACGTGC CACCTCCTAA GCCCGTGAAA 
ATGACAACTT TGCATTATCC TCTATTTAAT ATAAAATTAC AAATCCCAGC TGAAGATCAA 
AGCACTGATC AAGCGGATGC GGCACCTATA AAATCAAGAT CCGACCCAGC CCGAAAGCCC 
CGTGGCCGAA TTCCCAGGAG TCGGACCGGA AAATCACGTG AATCGGCGGC TGCTGAAGAA 
CTTATACAAA ACCAGACTCT GGAGCCGCCA CTGAAATCAG GCAAAGTCGA GTCATCGAGC 
TTGCCAAAGC CTCCAACAGA AGAAAAGATA GTGGAACCTG AGACTGGTGC CAAAGAAAAT 
GTTAATCAAT CTGCGCCCAG CGCCCAACCT GTTCTTATTG TTGAAGTGGA GAACGTAAAG 
CAACCGATCT ATAAACAAAC GGAAGAGGTG AAGGCATTAA CTCAGGAGAT AATCAAGACT 
CTTCGGGACA TAATCACAAT GAATCCCTTG TATAGAGAGA GTCTACAGCA GATGCTGCAC 
CAAAATCAAC GAGTTGTCGA CAACCCCATT TATCTGTGCG ATTTAGGAGC ATCCCTTTCC 
GCTGGAGAAC CAGCTGAGCT GCAAAAAATT CTTGAGGAAA CAGATATCCC AGAACGTCTA 
CAATTGGCCT TAACTTTACT CAAAAAGGAG TTGGAACTCT CCAGGCTCCA ACAAAAAATT 
GGACGCGAAG TAGAGGAAAA AGTCAAGCAG CAACATCGCA AATATATACT CCAGGAACAG 
TTGAAAGTCA TCAAAAAAGA ACTGGGCATC GAAAAAGACG ACAAGGATGC CATTGGTGAA 
AAGTACCGCG AAAAGCTGAA GGATAAGGTG GTTCCCGAAG CTATTATGAC GGTTATTGAT 
GAAGAACTCA CCAAGCTGAA CTTCCTGGAG AGTCATAGTT CCGAGTTTAA TGTAACTCGC 
AATTACCTCG ACTGGCTTAC TTCCCTACCT TGGGGCGTTA TCAGCACCGA AAATCTCTGC 
CTGGAGAAGG CCACTGAAAC ACTAAACGAC GATCACTACG GAATGGAGGA TATCAAGAAG 
CGTATTTTAG AATTTATTGC CGTCAGTTCC CTCAAGGGCT CTACGCAGGG CAAGATCTTG 
TGCTTTCATG GACCCCCTGG TGTGGGCAAG ACAAGCATAG CAAAGTCCAT TGCACGCGCT 
TTGAATCGCG AGTACTTCCG CTTTAGTGTG GGCGGCATGA CAGACGTTGC TGAGATCAAG 
GGACATCGCC GCACCTATGT GGGCGCAATG CCGGGTAAAC TGATTCAGTG CCTGAAGAAG 
ACTAAGATTG AAAATCCTCT GGTACTCATC GACGAGGTGG ACAAGATCGG CAAGGGCTAC 
CAGGGAGATC CTAGCTCAGC ATTGTTAGAG CTACTAGATC CCGAGCAGAA TGCGAATTTC 
CTGGACCACT ACCTCGATGT GCCGGTAGAC CTCTCGCGAG TGCTCTTCAT CTGCACAGCT 
AATGTGATCG ACACTATTCC CGAGCCTTTA AGAGATCGCA TGGAATTGAT TGAGATGTCT 
GGGTATGTTG CCGAAGAGAA GATCGCCATC GCTCGTCAGT ATCTGATGCC ACAAGCGATG 
AAGGACTGTG GATTAACGGA CAAGCATATC AACATTTCCG AGGATGCCCT AAACATGCTG 
ATTCGCAGTT ATTGCCGGGA ATCCGGGGTG CGAAACCTGC AAAAGCATAT CGAGAAAGTT 
ATTCGCAAGG TGGCCTTCCG AGTGGTCAAA AAAGAAGGCG AGCACTTTCC GGTTAACGCT 
GATAATCTGA CCACATTCCT GGGCAAACAA ATCTTTAGCT CAGATCGCAT GTACGCAACC 
ACACCGGTGG GTGTAGTCAT GGGTCTGGCC TGGACCGCCA TGGGTGGTTC ATCGCTTTAC 
ATCGAAACCT CAAGGCGTCA CATTCGCCAG GGGGCAAAAA CAGATCCGAA CACTGTCGCC 
GGATCGCTGC ACATCACCGG AAATTTGGGA GATGTCATGA AGGAGTCGGC CCAAATAGCC 
TTAACGGTGG CGCGCAACTT TTTATATTCG CTGGAACCCA ATAATCTGTT CTTAGAGCAA 
GAGCACATTC ACCTCCATGT TCCTGAAGGT GCCACACCGA AAGATGGCCC CAGTGCGGGC 
ATCACCATTA TTACAGCTCT CGTATCGTTG GCCACTGGAA AGCCGGTGCG CCAAGATATC 
GCTATGACGG GCGAAGTATC CCTGAAGGGA AAAGTGCTAC CCGTGGGTGG TATTAAGGAA 
AAGACGATTG CAGCACGTCG TAGTGGAGTG AATTGTCTTA TTCTGCCTGT GGATAACAAG 
AAAGACTTCG AGGAGTTGCC CACGTACATT ACAGATGGTC TGGAGGTTCA CTTTGCCACC 
ACCTACGAGG ATGTCTACAA GATAGCGTTT ACTGATGTAA CCGAAACGAC AACCAACAAT 
GTTGAAGAGC AGGAGCCGCT GCAGAAGCTT TCCAGTGCGG CCGCTGCCAA ATCGGAGACG 
TGGCCTTATT CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Lon cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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