Shal cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Shal
Entrez Gene ID	40129
Full Name	Shaker cognate l
Synonyms	CG9262,DNK[[v]]4,Dmel\CG9262,K[[v]]4,Kv4,Sh-l,Shl,dShal,shal
General protein information	Preferred Names Shaker cognate l Names CG9262 gene product from transcript CG9262-RB CG9262-PA CG9262-PB CG9262-PC Shaker-like Shal-PA Shal-PB Shal-PC shaker cognate 1 shaker cognate L shaker cognate l
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 76B7-76B8

mRNA and Protein(s)

mRNA	Protein	Name
NM_079435.4	NP_524159.1	shaker cognate l, isoform A
NM_001275126.1	NP_001262055.1	shaker cognate l, isoform C
NM_001104176.2	NP_001097646.1	shaker cognate l, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Shal	NP_001097646.1
Caenorhabditis elegans (roundworm)	shl-1	NP_500975.2
Pan troglodytes (chimpanzee)	KCND2	XP_001141749.1
	KCND2	XP_001082011.2
Mus musculus (house mouse)	Kcnd2	NP_062671.1
Rattus norvegicus (Norway rat)	Kcnd2	NP_113918.2
Gallus gallus (chicken)	KCND2	NP_989610.1
Xenopus tropicalis (tropical clawed frog)	kcnd2	XP_002935005.1
Bos taurus (cattle)	KCND2	NP_001178113.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007046	XP_308716.2
Homo sapiens (human)	KCND2	NP_036413.1
Canis lupus familiaris (dog)	KCND2	XP_852736.1
Danio rerio (zebrafish)	kcnd2	NP_001076336.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We found that ddaE neurons have an axonal diffusion barrier proximal to the cell body that requires a giant isoform of the neuronal ankyrin Ank2. Furthermore, the potassium channel shal concentrates in the proximal axon in an Ank2-dependent manner
Title: Bilaterian Giant Ankyrins Have a Common Evolutionary Origin and Play a Conserved Role in Patterning the Axon Initial Segment.

The loss of Shal potassium channel induces compensatory rebalancing of ion channel expression.
Title: Kr?ppel mediates the selective rebalancing of ion channel expression.

In Drosophila, the single Shal/K(v)4 gene encodes the predominant I(A) current in many neuronal cell bodies.
Title: Shal/K(v)4 channels are required for maintaining excitability during repetitive firing and normal locomotion in Drosophila.

Data show that SIDL is expressed primarily in the nervous system, co-localizes with GFP-Shal channels in neurons, and interacts specifically with the LL-motif of Drosophila and mouse Shal channels.
Title: SIDL interacts with the dendritic targeting motif of Shal (K(v)4) K+ channels in Drosophila.

The following Shal gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Shal cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18977	NM_001104176.2 Latest version!	Drosophila melanogaster shaker cognate l, transcript variant B (Shal), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa18978	NM_079435.4 Latest version!	Drosophila melanogaster shaker cognate l, transcript variant A (Shal), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa21086	NM_001275126.1 Latest version!	Drosophila melanogaster shaker cognate l, transcript variant C (Shal), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Shal ( NM_001104176.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097646.1 Drosophila melanogaster shaker cognate l, transcript variant B (Shal), mRNA.

Price & Availability↑

CloneID	OFa18977
Clone ID Related Accession (Same CDS sequence)	NM_001104176.2
Accession Version	NM_001104176.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shaker cognate l, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001104176.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTCGG TCGCCGCTTG GCTGCCCTTC GCCCGGGCGG CGGCCATCGG GTGGGTGCCG 
ATAGCCACCC ACCCACTGCC ACCGCCCCCG ATGCCCAAGG ATCGCCGCAA AACGGACGAC 
GAGAAGCTCC TGATCAACGT CTCCGGGCGG CGTTTCGAGA CGTGGCGGAA TACTTTGGAG 
AAGTATCCGG ACACCCTTTT AGGTTCCAAT GAAAGGGAGT TCTTCTACGA CGAGGACTGC 
AAAGAATACT TCTTCGATCG GGACCCGGAC ATCTTCCGGC ACATACTGAA CTACTACCGG 
ACGGGCAAGC TGCACTACCC GAAGCACGAA TGCCTCACCA GCTACGACGA GGAGCTGGCC 
TTCTTTGGAA TAATGCCGGA TGTCATTGGC GATTGCTGCT ACGAGGACTA CCGGGACCGG 
AAGCGGGAGA ACGCGGAGCG GCTGATGGAC GACAAGCTGT CGGAGAACGG GGATCAGAAT 
CTGCAGCAGC TGACCAACAT GCGCCAGAAG ATGTGGCGGG CCTTCGAGAA TCCGCACACG 
TCGACGAGCG CCCTGGTGTT CTACTATGTT ACGGGTTTCT TCATCGCCGT CTCCGTGATG 
GCCAACGTGG TGGAGACGGT GCCGTGTGGC CACCGGCCGG GCAGAGCGGG AACTCTGCCC 
TGCGGCGAGC GCTACAAGAT CGTCTTCTTC TGCCTGGATA CCGCCTGCGT GATGATCTTT 
ACGGCGGAGT ACCTACTTCG ACTCTTCGCC GCCCCCGATC GCTGCAAGTT CGTGCGCTCG 
GTGATGAGCA TTATTGATGT GGTGGCCATT ATGCCGTACT ACATTGGCCT CGGGATCACC 
GACAACGACG ACGTGAGCGG TGCTTTCGTC ACGCTGCGCG TGTTCCGTGT CTTCCGCATA 
TTCAAGTTCT CGCGCCACTC GCAAGGACTT CGGATCCTCG GCTACACGCT CAAGTCCTGC 
GCCAGCGAAC TGGGCTTCCT TGTCTTCTCG CTGGCCATGG CCATTATCAT CTTTGCCACC 
GTCATGTTCT ACGCCGAGAA GAACGTCAAT GGCACCAACT TCACATCGAT TCCGGCGGCC 
TTCTGGTATA CCATCGTCAC AATGACGACG CTGGGATATG GCGACATGGT GCCAGAGACA 
ATAGCTGGCA AAATTGTGGG CGGCGTCTGC TCGCTTAGCG GTGTGCTGGT CATCGCCTTA 
CCTGTACCTG TTATCGTATC GAACTTTAGT AGAATCTATC ACCAGAACCA GCGAGCGGAC 
AAGCGCAAGG CGCAGCGGAA AGCTCGCCTG GCGCGCATCC GCATTGCCAA GGCCTCGTCC 
GGAGCCGCCT TTGTTAGCAA GAAGAAGGCC GCCGAGGCCC GGTGGGCTGC CCAGGAGTCG 
GGCATCGAGC TGGATGACAA CTATCGGGAC GAGGACATCT TCGAGCTGCA GCACCATCAT 
TTGCTGCGAT GTCTGGAGAA GACAACGGAT CGAGAATTTG TCGAGTTAGA AATACCGTTT 
AACGGCCAGC CCAAGAGGCC GGGCTCCCCG TCCCCGATGG CCAGTCCCGC CCACTCGACA 
AACAGTGCCG CCGGCCTGCT GCAGTCCTGC TGCGGCCGAT GCTGCTCCCA GCGCTACCAG 
GCCTGCGGCA AGTACATGCC GGCTGCCAGC AATGCTCAGA ATAGCCAAAA CAATCAGCCC 
ATGGATGGCA CCTACCTGGT GGAGGCCTCG TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097646.1
CDS	612..2327
Translation	MASVAAWLPFARAAAIGWVPIATHPLPPPPMPKDRRKTDDEKLLINVSGRRFETWRNTLEKYPDTLLGSNEREFFYDEDCKEYFFDRDPDIFRHILNYYRTGKLHYPKHECLTSYDEELAFFGIMPDVIGDCCYEDYRDRKRENAERLMDDKLSENGDQNLQQLTNMRQKMWRAFENPHTSTSALVFYYVTGFFIAVSVMANVVETVPCGHRPGRAGTLPCGERYKIVFFCLDTACVMIFTAEYLLRLFAAPDRCKFVRSVMSIIDVVAIMPYYIGLGITDNDDVSGAFVTLRVFRVFRIFKFSRHSQGLRILGYTLKSCASELGFLVFSLAMAIIIFATVMFYAEKNVNGTNFTSIPAAFWYTIVTMTTLGYGDMVPETIAGKIVGGVCSLSGVLVIALPVPVIVSNFSRIYHQNQRADKRKAQRKARLARIRIAKASSGAAFVSKKKAAEARWAAQESGIELDDNYRDEDIFELQHHHLLRCLEKTTDREFVELEIPFNGQPKRPGSPSPMASPAHSTNSAAGLLQSCCGRCCSQRYQACGKYMPAASNAQNSQNNQPMDGTYLVEASF

Target ORF information:

RefSeq Version	NM_001104176.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shaker cognate l, transcript variant B (Shal), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104176.2

ORF Insert Sequence:

ATGGCCTCGG TCGCCGCTTG GCTGCCCTTC GCCCGGGCGG CGGCCATCGG GTGGGTGCCG 
ATAGCCACCC ACCCACTGCC ACCGCCCCCG ATGCCCAAGG ATCGCCGCAA AACGGACGAC 
GAGAAGCTCC TGATCAACGT CTCCGGGCGG CGTTTCGAGA CGTGGCGGAA TACTTTGGAG 
AAGTATCCGG ACACCCTTTT AGGTTCCAAT GAAAGGGAGT TCTTCTACGA CGAGGACTGC 
AAAGAATACT TCTTCGATCG GGACCCGGAC ATCTTCCGGC ACATACTGAA CTACTACCGG 
ACGGGCAAGC TGCACTACCC GAAGCACGAA TGCCTCACCA GCTACGACGA GGAGCTGGCC 
TTCTTTGGAA TAATGCCGGA TGTCATTGGC GATTGCTGCT ACGAGGACTA CCGGGACCGG 
AAGCGGGAGA ACGCGGAGCG GCTGATGGAC GACAAGCTGT CGGAGAACGG GGATCAGAAT 
CTGCAGCAGC TGACCAACAT GCGCCAGAAG ATGTGGCGGG CCTTCGAGAA TCCGCACACG 
TCGACGAGCG CCCTGGTGTT CTACTATGTT ACGGGTTTCT TCATCGCCGT CTCCGTGATG 
GCCAACGTGG TGGAGACGGT GCCGTGTGGC CACCGGCCGG GCAGAGCGGG AACTCTGCCC 
TGCGGCGAGC GCTACAAGAT CGTCTTCTTC TGCCTGGATA CCGCCTGCGT GATGATCTTT 
ACGGCGGAGT ACCTACTTCG ACTCTTCGCC GCCCCCGATC GCTGCAAGTT CGTGCGCTCG 
GTGATGAGCA TTATTGATGT GGTGGCCATT ATGCCGTACT ACATTGGCCT CGGGATCACC 
GACAACGACG ACGTGAGCGG TGCTTTCGTC ACGCTGCGCG TGTTCCGTGT CTTCCGCATA 
TTCAAGTTCT CGCGCCACTC GCAAGGACTT CGGATCCTCG GCTACACGCT CAAGTCCTGC 
GCCAGCGAAC TGGGCTTCCT TGTCTTCTCG CTGGCCATGG CCATTATCAT CTTTGCCACC 
GTCATGTTCT ACGCCGAGAA GAACGTCAAT GGCACCAACT TCACATCGAT TCCGGCGGCC 
TTCTGGTATA CCATCGTCAC AATGACGACG CTGGGATATG GCGACATGGT GCCAGAGACA 
ATAGCTGGCA AAATTGTGGG CGGCGTCTGC TCGCTTAGCG GTGTGCTGGT CATCGCCTTA 
CCTGTACCTG TTATCGTATC GAACTTTAGT AGAATCTATC ACCAGAACCA GCGAGCGGAC 
AAGCGCAAGG CGCAGCGGAA AGCTCGCCTG GCGCGCATCC GCATTGCCAA GGCCTCGTCC 
GGAGCCGCCT TTGTTAGCAA GAAGAAGGCC GCCGAGGCCC GGTGGGCTGC CCAGGAGTCG 
GGCATCGAGC TGGATGACAA CTATCGGGAC GAGGACATCT TCGAGCTGCA GCACCATCAT 
TTGCTGCGAT GTCTGGAGAA GACAACGGAT CGAGAATTTG TCGAGTTAGA AATACCGTTT 
AACGGCCAGC CCAAGAGGCC GGGCTCCCCG TCCCCGATGG CCAGTCCCGC CCACTCGACA 
AACAGTGCCG CCGGCCTGCT GCAGTCCTGC TGCGGCCGAT GCTGCTCCCA GCGCTACCAG 
GCCTGCGGCA AGTACATGCC GGCTGCCAGC AATGCTCAGA ATAGCCAAAA CAATCAGCCC 
ATGGATGGCA CCTACCTGGT GGAGGCCTCG TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shal ( NM_079435.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524159.1 Drosophila melanogaster shaker cognate l, transcript variant A (Shal), mRNA.

Price & Availability↑

CloneID	OFa18978
Clone ID Related Accession (Same CDS sequence)	NM_079435.4
Accession Version	NM_079435.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shaker cognate l, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_079435.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTCGG TCGCCGCTTG GCTGCCCTTC GCCCGGGCGG CGGCCATCGG GTGGGTGCCG 
ATAGCCACCC ACCCACTGCC ACCGCCCCCG ATGCCCAAGG ATCGCCGCAA AACGGACGAC 
GAGAAGCTCC TGATCAACGT CTCCGGGCGG CGTTTCGAGA CGTGGCGGAA TACTTTGGAG 
AAGTATCCGG ACACCCTTTT AGGTTCCAAT GAAAGGGAGT TCTTCTACGA CGAGGACTGC 
AAAGAATACT TCTTCGATCG GGACCCGGAC ATCTTCCGGC ACATACTGAA CTACTACCGG 
ACGGGCAAGC TGCACTACCC GAAGCACGAA TGCCTCACCA GCTACGACGA GGAGCTGGCC 
TTCTTTGGAA TAATGCCGGA TGTCATTGGC GATTGCTGCT ACGAGGACTA CCGGGACCGG 
AAGCGGGAGA ACGCGGAGCG GCTGATGGAC GACAAGCTGT CGGAGAACGG GGATCAGAAT 
CTGCAGCAGC TGACCAACAT GCGCCAGAAG ATGTGGCGGG CCTTCGAGAA TCCGCACACG 
TCGACGAGCG CCCTGGTGTT CTACTATGTT ACGGGTTTCT TCATCGCCGT CTCCGTGATG 
GCCAACGTGG TGGAGACGGT GCCGTGTGGC CACCGGCCGG GCAGAGCGGG AACTCTGCCC 
TGCGGCGAGC GCTACAAGAT CGTCTTCTTC TGCCTGGATA CCGCCTGCGT GATGATCTTT 
ACGGCGGAGT ACCTACTTCG ACTCTTCGCC GCCCCCGATC GCTGCAAGTT CGTGCGCTCG 
GTGATGAGCA TTATTGATGT GGTGGCCATT ATGCCGTACT ACATTGGCCT CGGGATCACC 
GACAACGACG ACGTGAGCGG TGCTTTCGTC ACGCTGCGCG TGTTCCGTGT CTTCCGCATA 
TTCAAGTTCT CGCGCCACTC GCAAGGACTT CGGATCCTCG GCTACACGCT CAAGTCCTGC 
GCCAGCGAAC TGGGCTTCCT TGTCTTCTCG CTGGCCATGG CCATTATCAT CTTTGCCACC 
GTCATGTTCT ACGCCGAGAA GAACGTCAAT GGCACCAACT TCACATCGAT TCCGGCGGCC 
TTCTGGTATA CCATCGTCAC AATGACGACG CTGGGATATG GCGACATGGT GCCAGAGACA 
ATAGCTGGCA AAATTGTGGG CGGCGTCTGC TCGCTTAGCG GTGTGCTGGT CATCGCCTTA 
CCTGTACCTG TTATCGTATC GAACTTTAGT AGAATCTATC ACCAGAACCA GCGAGCGGAC 
AAGCGCAAGG CGCAGCGGAA AGCTCGCCTG GCGCGCATCC GCATTGCCAA GGCCTCGTCC 
GGAGCCGCCT TTGTTAGCAA GAAGAAGGCC GCCGAGGCCC GGTGGGCTGC CCAGGAGTCG 
GGCATCGAGC TGGATGACAA CTATCGGGAC GAGGACATCT TCGAGCTGCA GCACCATCAT 
TTGCTGCGAT GTCTGGAGAA GACAACGATG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524159.1
CDS	623..2095
Translation	MASVAAWLPFARAAAIGWVPIATHPLPPPPMPKDRRKTDDEKLLINVSGRRFETWRNTLEKYPDTLLGSNEREFFYDEDCKEYFFDRDPDIFRHILNYYRTGKLHYPKHECLTSYDEELAFFGIMPDVIGDCCYEDYRDRKRENAERLMDDKLSENGDQNLQQLTNMRQKMWRAFENPHTSTSALVFYYVTGFFIAVSVMANVVETVPCGHRPGRAGTLPCGERYKIVFFCLDTACVMIFTAEYLLRLFAAPDRCKFVRSVMSIIDVVAIMPYYIGLGITDNDDVSGAFVTLRVFRVFRIFKFSRHSQGLRILGYTLKSCASELGFLVFSLAMAIIIFATVMFYAEKNVNGTNFTSIPAAFWYTIVTMTTLGYGDMVPETIAGKIVGGVCSLSGVLVIALPVPVIVSNFSRIYHQNQRADKRKAQRKARLARIRIAKASSGAAFVSKKKAAEARWAAQESGIELDDNYRDEDIFELQHHHLLRCLEKTTM

Target ORF information:

RefSeq Version	NM_079435.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shaker cognate l, transcript variant A (Shal), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079435.4

ORF Insert Sequence:

ATGGCCTCGG TCGCCGCTTG GCTGCCCTTC GCCCGGGCGG CGGCCATCGG GTGGGTGCCG 
ATAGCCACCC ACCCACTGCC ACCGCCCCCG ATGCCCAAGG ATCGCCGCAA AACGGACGAC 
GAGAAGCTCC TGATCAACGT CTCCGGGCGG CGTTTCGAGA CGTGGCGGAA TACTTTGGAG 
AAGTATCCGG ACACCCTTTT AGGTTCCAAT GAAAGGGAGT TCTTCTACGA CGAGGACTGC 
AAAGAATACT TCTTCGATCG GGACCCGGAC ATCTTCCGGC ACATACTGAA CTACTACCGG 
ACGGGCAAGC TGCACTACCC GAAGCACGAA TGCCTCACCA GCTACGACGA GGAGCTGGCC 
TTCTTTGGAA TAATGCCGGA TGTCATTGGC GATTGCTGCT ACGAGGACTA CCGGGACCGG 
AAGCGGGAGA ACGCGGAGCG GCTGATGGAC GACAAGCTGT CGGAGAACGG GGATCAGAAT 
CTGCAGCAGC TGACCAACAT GCGCCAGAAG ATGTGGCGGG CCTTCGAGAA TCCGCACACG 
TCGACGAGCG CCCTGGTGTT CTACTATGTT ACGGGTTTCT TCATCGCCGT CTCCGTGATG 
GCCAACGTGG TGGAGACGGT GCCGTGTGGC CACCGGCCGG GCAGAGCGGG AACTCTGCCC 
TGCGGCGAGC GCTACAAGAT CGTCTTCTTC TGCCTGGATA CCGCCTGCGT GATGATCTTT 
ACGGCGGAGT ACCTACTTCG ACTCTTCGCC GCCCCCGATC GCTGCAAGTT CGTGCGCTCG 
GTGATGAGCA TTATTGATGT GGTGGCCATT ATGCCGTACT ACATTGGCCT CGGGATCACC 
GACAACGACG ACGTGAGCGG TGCTTTCGTC ACGCTGCGCG TGTTCCGTGT CTTCCGCATA 
TTCAAGTTCT CGCGCCACTC GCAAGGACTT CGGATCCTCG GCTACACGCT CAAGTCCTGC 
GCCAGCGAAC TGGGCTTCCT TGTCTTCTCG CTGGCCATGG CCATTATCAT CTTTGCCACC 
GTCATGTTCT ACGCCGAGAA GAACGTCAAT GGCACCAACT TCACATCGAT TCCGGCGGCC 
TTCTGGTATA CCATCGTCAC AATGACGACG CTGGGATATG GCGACATGGT GCCAGAGACA 
ATAGCTGGCA AAATTGTGGG CGGCGTCTGC TCGCTTAGCG GTGTGCTGGT CATCGCCTTA 
CCTGTACCTG TTATCGTATC GAACTTTAGT AGAATCTATC ACCAGAACCA GCGAGCGGAC 
AAGCGCAAGG CGCAGCGGAA AGCTCGCCTG GCGCGCATCC GCATTGCCAA GGCCTCGTCC 
GGAGCCGCCT TTGTTAGCAA GAAGAAGGCC GCCGAGGCCC GGTGGGCTGC CCAGGAGTCG 
GGCATCGAGC TGGATGACAA CTATCGGGAC GAGGACATCT TCGAGCTGCA GCACCATCAT 
TTGCTGCGAT GTCTGGAGAA GACAACGATG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Shal ( NM_001275126.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262055.1 Drosophila melanogaster shaker cognate l, transcript variant C (Shal), mRNA.

Price & Availability↑

CloneID	OFa21086
Clone ID Related Accession (Same CDS sequence)	NM_001275126.1
Accession Version	NM_001275126.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shaker cognate l, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTCGG TCGCCGCTTG GCTGCCCTTC GCCCGGGCGG CGGCCATCGG GTGGGTGCCG 
ATAGCCACCC ACCCACTGCC ACCGCCCCCG ATGCCCAAGG ATCGCCGCAA AACGGACGAC 
GAGAAGCTCC TGATCAACGT CTCCGGGCGG CGTTTCGAGA CGTGGCGGAA TACTTTGGAG 
AAGTATCCGG ACACCCTTTT AGGTTCCAAT GAAAGGGAGT TCTTCTACGA CGAGGACTGC 
AAAGAATACT TCTTCGATCG GGACCCGGAC ATCTTCCGGC ACATACTGAA CTACTACCGG 
ACGGGCAAGC TGCACTACCC GAAGCACGAA TGCCTCACCA GCTACGACGA GGAGCTGGCC 
TTCTTTGGAA TAATGCCGGA TGTCATTGGC GATTGCTGCT ACGAGGACTA CCGGGACCGG 
AAGCGGGAGA ACGCGGAGCG GCTGATGGAC GACAAGCTGT CGGAGAACGG GGATCAGAAT 
CTGCAGCAGC TGACCAACAT GCGCCAGAAG ATGTGGCGGG CCTTCGAGAA TCCGCACACG 
TCGACGAGCG CCCTGGTGTT CTACTATGTT ACGGGTTTCT TCATCGCCGT CTCCGTGATG 
GCCAACGTGG TGGAGACGGT GCCGTGTGGC CACCGGCCGG GCAGAGCGGG AACTCTGCCC 
TGCGGCGAGC GCTACAAGAT CGTCTTCTTC TGCCTGGATA CCGCCTGCGT GATGATCTTT 
ACGGCGGAGT ACCTACTTCG ACTCTTCGCC GCCCCCGATC GCTGCAAGTT CGTGCGCTCG 
GTGATGAGCA TTATTGATGT GGTGGCCATT ATGCCGTACT ACATTGGCCT CGGGATCACC 
GACAACGACG ACGTGAGCGG TGCTTTCGTC ACGCTGCGCG TGTTCCGTGT CTTCCGCATA 
TTCAAGTTCT CGCGCCACTC GCAAGGACTT CGGATCCTCG GCTACACGCT CAAGTCCTGC 
GCCAGCGAAC TGGGCTTCCT TGTCTTCTCG CTGGCCATGG CCATTATCAT CTTTGCCACC 
GTCATGTTCT ACGCCGAGAA GAACGTCAAT GGCACCAACT TCACATCGAT TCCGGCGGCC 
TTCTGGTATA CCATCGTCAC AATGACGACG CTGGGATATG GCGACATGGT GCCAGAGACA 
ATAGCTGGCA AAATTGTGGG CGGCGTCTGC TCGCTTAGCG GTGTGCTGGT CATCGCCTTA 
CCTGTACCTG TTATCGTATC GAACTTTAGT AGAATCTATC ACCAGAACCA GCGAGCGGAC 
AAGCGCAAGG CGCAGCGGAA AGCTCGCCTG GCGCGCATCC GCATTGCCAA GGCCTCGTCC 
GGAGCCGCCT TTGTTAGCAA GAAGAAGGCC GCCGAGGCCC GGTGGGCTGC CCAGGAGTCG 
GGCATCGAGC TGGATGACAA CTATCGGGAC GAGGACATCT TCGAGCTGCA GCACCATCAT 
TTGCTGCGAT GTCTGGAGAA GACAACGGCC TGCGGCAAGT ACATGCCGGC TGCCAGCAAT 
GCTCAGAATA GCCAAAACAA TCAGCCCATG GATGGCACCT ACCTGGTGGA GGCCTCGTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262055.1
CDS	612..2174
Translation	MASVAAWLPFARAAAIGWVPIATHPLPPPPMPKDRRKTDDEKLLINVSGRRFETWRNTLEKYPDTLLGSNEREFFYDEDCKEYFFDRDPDIFRHILNYYRTGKLHYPKHECLTSYDEELAFFGIMPDVIGDCCYEDYRDRKRENAERLMDDKLSENGDQNLQQLTNMRQKMWRAFENPHTSTSALVFYYVTGFFIAVSVMANVVETVPCGHRPGRAGTLPCGERYKIVFFCLDTACVMIFTAEYLLRLFAAPDRCKFVRSVMSIIDVVAIMPYYIGLGITDNDDVSGAFVTLRVFRVFRIFKFSRHSQGLRILGYTLKSCASELGFLVFSLAMAIIIFATVMFYAEKNVNGTNFTSIPAAFWYTIVTMTTLGYGDMVPETIAGKIVGGVCSLSGVLVIALPVPVIVSNFSRIYHQNQRADKRKAQRKARLARIRIAKASSGAAFVSKKKAAEARWAAQESGIELDDNYRDEDIFELQHHHLLRCLEKTTACGKYMPAASNAQNSQNNQPMDGTYLVEASF

Target ORF information:

RefSeq Version	NM_001275126.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shaker cognate l, transcript variant C (Shal), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275126.1

ORF Insert Sequence:

ATGGCCTCGG TCGCCGCTTG GCTGCCCTTC GCCCGGGCGG CGGCCATCGG GTGGGTGCCG 
ATAGCCACCC ACCCACTGCC ACCGCCCCCG ATGCCCAAGG ATCGCCGCAA AACGGACGAC 
GAGAAGCTCC TGATCAACGT CTCCGGGCGG CGTTTCGAGA CGTGGCGGAA TACTTTGGAG 
AAGTATCCGG ACACCCTTTT AGGTTCCAAT GAAAGGGAGT TCTTCTACGA CGAGGACTGC 
AAAGAATACT TCTTCGATCG GGACCCGGAC ATCTTCCGGC ACATACTGAA CTACTACCGG 
ACGGGCAAGC TGCACTACCC GAAGCACGAA TGCCTCACCA GCTACGACGA GGAGCTGGCC 
TTCTTTGGAA TAATGCCGGA TGTCATTGGC GATTGCTGCT ACGAGGACTA CCGGGACCGG 
AAGCGGGAGA ACGCGGAGCG GCTGATGGAC GACAAGCTGT CGGAGAACGG GGATCAGAAT 
CTGCAGCAGC TGACCAACAT GCGCCAGAAG ATGTGGCGGG CCTTCGAGAA TCCGCACACG 
TCGACGAGCG CCCTGGTGTT CTACTATGTT ACGGGTTTCT TCATCGCCGT CTCCGTGATG 
GCCAACGTGG TGGAGACGGT GCCGTGTGGC CACCGGCCGG GCAGAGCGGG AACTCTGCCC 
TGCGGCGAGC GCTACAAGAT CGTCTTCTTC TGCCTGGATA CCGCCTGCGT GATGATCTTT 
ACGGCGGAGT ACCTACTTCG ACTCTTCGCC GCCCCCGATC GCTGCAAGTT CGTGCGCTCG 
GTGATGAGCA TTATTGATGT GGTGGCCATT ATGCCGTACT ACATTGGCCT CGGGATCACC 
GACAACGACG ACGTGAGCGG TGCTTTCGTC ACGCTGCGCG TGTTCCGTGT CTTCCGCATA 
TTCAAGTTCT CGCGCCACTC GCAAGGACTT CGGATCCTCG GCTACACGCT CAAGTCCTGC 
GCCAGCGAAC TGGGCTTCCT TGTCTTCTCG CTGGCCATGG CCATTATCAT CTTTGCCACC 
GTCATGTTCT ACGCCGAGAA GAACGTCAAT GGCACCAACT TCACATCGAT TCCGGCGGCC 
TTCTGGTATA CCATCGTCAC AATGACGACG CTGGGATATG GCGACATGGT GCCAGAGACA 
ATAGCTGGCA AAATTGTGGG CGGCGTCTGC TCGCTTAGCG GTGTGCTGGT CATCGCCTTA 
CCTGTACCTG TTATCGTATC GAACTTTAGT AGAATCTATC ACCAGAACCA GCGAGCGGAC 
AAGCGCAAGG CGCAGCGGAA AGCTCGCCTG GCGCGCATCC GCATTGCCAA GGCCTCGTCC 
GGAGCCGCCT TTGTTAGCAA GAAGAAGGCC GCCGAGGCCC GGTGGGCTGC CCAGGAGTCG 
GGCATCGAGC TGGATGACAA CTATCGGGAC GAGGACATCT TCGAGCTGCA GCACCATCAT 
TTGCTGCGAT GTCTGGAGAA GACAACGGCC TGCGGCAAGT ACATGCCGGC TGCCAGCAAT 
GCTCAGAATA GCCAAAACAA TCAGCCCATG GATGGCACCT ACCTGGTGGA GGCCTCGTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Shal cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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