not cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	not
Entrez Gene ID	40030
Full Name	non-stop
Synonyms	CG4166,Dmel\CG4166,NOT,Nonstop,Not,Q9VVR1,UBP8,USP22,Upb8,anon-WO0257455.9,i155,l(3)02069,l(3)j9A6,nonstop
General protein information	Preferred Names non-stop Names CG4166 gene product from transcript CG4166-RC CG4166-PB CG4166-PC Non-stop nonstop not-PB not-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 75D4-75D4

mRNA and Protein(s)

mRNA	Protein	Name
NM_079416.4	NP_524140.2	non-stop, isoform B
NM_001300177.1	NP_001287106.1	non-stop, isoform C

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	USP22	XP_005620237.1
Bos taurus (cattle)	USP22	NP_001160039.1
Gallus gallus (chicken)	USP22	XP_004945238.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012119	XP_320409.4
Saccharomyces cerevisiae (baker's yeast)	UBP8	NP_013950.1
Danio rerio (zebrafish)	usp22	NP_001038713.1
Schizosaccharomyces pombe (fission yeast)	ubp8	NP_592992.1
Xenopus tropicalis (tropical clawed frog)	usp22	NP_001192175.1
Eremothecium gossypii	AGOS_AFR551W	NP_986098.2
Neurospora crassa	NCU02375	XP_959796.2
Pan troglodytes (chimpanzee)	USP22	XP_003953155.1
Mus musculus (house mouse)	Usp22	NP_001004143.2
Drosophila melanogaster (fruit fly)	not	NP_524140.2
Oryza sativa (rice)	Os04g0647300	NP_001054070.2
Homo sapiens (human)	USP22	NP_056091.1
Rattus norvegicus (Norway rat)	Usp22	NP_001178573.1
Kluyveromyces lactis	KLLA0C06974g	XP_452509.1
Magnaporthe oryzae (rice blast fungus)	MGG_03527	XP_003716392.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Loss of Ataxin-7 results in a toxic gain of activity of the Non-stop deubiquitinase.
Title: Drosophila models reveal novel insights into mechanisms underlying neurodegeneration.

Data establish that Nonstop actually functions as an ubiquitin protease to control the levels of ubiquitinated histone H2B in flies. We further establish that Nonstop is the functional homolog of yeast Ubp8
Title: SAGA-mediated H2B deubiquitination controls the development of neuronal connectivity in the Drosophila visual system.

3 additional subunits of TFTC/STAGA (dSgf11, non-stop, & en(y)2) help form the deubiquitination module. This module is an enhancer of position effect variegation in Drosophila.
Title: A TFTC/STAGA module mediates histone H2A and H2B deubiquitination, coactivates nuclear receptors, and counteracts heterochromatin silencing.

The following not gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the not cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18870	NM_001300177.1 Latest version!	Drosophila melanogaster non-stop (not), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa18870	NM_079416.4 Latest version!	Drosophila melanogaster non-stop (not), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

not ( NM_079416.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524140.2 Drosophila melanogaster non-stop (not), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18870
Clone ID Related Accession (Same CDS sequence)	NM_079416.4 , NM_001300177.1
Accession Version	NM_079416.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	non-stop, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079416.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGAGA CGGGTTGTCG GCACTATCAA AGCTATGTGA AGGAGCACAG CTACGATACA 
TTCCGGGTCA TCGACGCCTA CTTCGCTGCT TGCGTCAACA GAGATGCGCG CGAGCGAAAG 
GCTATTCACT GCAACTGCTT CGAGTGCGGC AGCTACGGCA TCCAGTTGTA CGCTTGCCTG 
CACTGCATCT ACTTCGGATG TCGGGGGGCC CACATCACGA GTCACCTGCG ATCCAAGAAG 
CACAATGTGG CCCTGGAGCT GTCCCACGGA ACGCTCTACT GCTACGCCTG CAGAGATTTC 
ATCTACGACG CGCGGAGTCG CGAGTATGCG CTGATCAACC GCAAGCTGGA GGCGAAGGAT 
CTGCAGAAGA GCATCGGCTG GGTGCCGTGG GTTCCCACAA CCAAGGAGAC CAATCTGCTG 
CTGGCCAATG CGAGGCGTCG GCTGGTGAGG CCCAATCAAA CGATCGGACT GAGGGGTCTG 
CTTAATCTGG GCGCCACTTG CTTCATGAAC TGCATCGTCC AGGCTCTGGT TCACACGCCC 
CTGCTCAGCG ATTACTTTAT GTCAGATCGT CACGACTGCG GCAGCAAGTC ATCGCACAAA 
TGTCTCGTTT GTGAAGTGTC ACGTCTCTTC CAGGAGTTCT ATTCCGGCTC CCGTTCGCCG 
CTGTCGCTGC ACCGGCTACT GCATTTAATC TGGAACCATG CGAAGCACTT GGCCGGCTAC 
GAGCAGCAGG ATGCGCACGA GTTCTTCATC GCCACTCTGG ACGTGCTGCA TCGGCACTGC 
GTGAAGGCAA AGGCGGAGCA CGAAAGCAAG AGCAACAGCT CTGGCTCGGG AAGTGGAACC 
AATTCCAGCA ACTCGAGCAG CTCGCATTGC TACGGCCAGT GTAATTGCAT CATTGACCAG 
ATCTTCACGG GCATGCTGCA GAGCGATGTG GTGTGCCAGG CCTGCAACGG AGTGTCCACC 
ACGTACGATC CGTTTTGGGA CATTTCGCTG GACCTGGGCG AAACAACAAC GCATGGTGGT 
GTTACTCCAA AAACGCTGAT AGACTGCCTG GAGCGGTACA CTCGGGCCGA GCATCTCGGA 
TCGGCGGCCA AAATCAAGTG CTCTACCTGC AAATCCTACC AGGAGTCCAC CAAACAGTTC 
AGCCTGCGCA CTCTGCCCAG CGTGGTGTCG TTCCATTTGA AACGCTTCGA GCACTCAGCT 
CTGATCGATC GCAAGATCTC CTCGTTCATT CAATTCCCCG TGGAGTTCGA CATGACGCCC 
TTTATGTCGG AGAAGAAGAA CGCCTACGGC GACTTCCGGT TCTCTCTGTA CGCCGTGGTC 
AATCATGTGG GCACCATCGA CACCGGCCAC TATACGGCGT ATGTGCGGCA CCAGAAGGAC 
ACGTGGGTCA AGTGCGATGA TCATGTGATA ACGATGGCAT CGCTCAAGCA GGTGCTGGAC 
AGCGAGGGTT ATTTATTGTT CTACCATAAA AATGTGCTGG AATACGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524140.2
CDS	639..2129
Translation	MSETGCRHYQSYVKEHSYDTFRVIDAYFAACVNRDARERKAIHCNCFECGSYGIQLYACLHCIYFGCRGAHITSHLRSKKHNVALELSHGTLYCYACRDFIYDARSREYALINRKLEAKDLQKSIGWVPWVPTTKETNLLLANARRRLVRPNQTIGLRGLLNLGATCFMNCIVQALVHTPLLSDYFMSDRHDCGSKSSHKCLVCEVSRLFQEFYSGSRSPLSLHRLLHLIWNHAKHLAGYEQQDAHEFFIATLDVLHRHCVKAKAEHESKSNSSGSGSGTNSSNSSSSHCYGQCNCIIDQIFTGMLQSDVVCQACNGVSTTYDPFWDISLDLGETTTHGGVTPKTLIDCLERYTRAEHLGSAAKIKCSTCKSYQESTKQFSLRTLPSVVSFHLKRFEHSALIDRKISSFIQFPVEFDMTPFMSEKKNAYGDFRFSLYAVVNHVGTIDTGHYTAYVRHQKDTWVKCDDHVITMASLKQVLDSEGYLLFYHKNVLEYE

Target ORF information:

RefSeq Version	NM_079416.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster non-stop (not), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079416.4

ORF Insert Sequence:

ATGTCCGAGA CGGGTTGTCG GCACTATCAA AGCTATGTGA AGGAGCACAG CTACGATACA 
TTCCGGGTCA TCGACGCCTA CTTCGCTGCT TGCGTCAACA GAGATGCGCG CGAGCGAAAG 
GCTATTCACT GCAACTGCTT CGAGTGCGGC AGCTACGGCA TCCAGTTGTA CGCTTGCCTG 
CACTGCATCT ACTTCGGATG TCGGGGGGCC CACATCACGA GTCACCTGCG ATCCAAGAAG 
CACAATGTGG CCCTGGAGCT GTCCCACGGA ACGCTCTACT GCTACGCCTG CAGAGATTTC 
ATCTACGACG CGCGGAGTCG CGAGTATGCG CTGATCAACC GCAAGCTGGA GGCGAAGGAT 
CTGCAGAAGA GCATCGGCTG GGTGCCGTGG GTTCCCACAA CCAAGGAGAC CAATCTGCTG 
CTGGCCAATG CGAGGCGTCG GCTGGTGAGG CCCAATCAAA CGATCGGACT GAGGGGTCTG 
CTTAATCTGG GCGCCACTTG CTTCATGAAC TGCATCGTCC AGGCTCTGGT TCACACGCCC 
CTGCTCAGCG ATTACTTTAT GTCAGATCGT CACGACTGCG GCAGCAAGTC ATCGCACAAA 
TGTCTCGTTT GTGAAGTGTC ACGTCTCTTC CAGGAGTTCT ATTCCGGCTC CCGTTCGCCG 
CTGTCGCTGC ACCGGCTACT GCATTTAATC TGGAACCATG CGAAGCACTT GGCCGGCTAC 
GAGCAGCAGG ATGCGCACGA GTTCTTCATC GCCACTCTGG ACGTGCTGCA TCGGCACTGC 
GTGAAGGCAA AGGCGGAGCA CGAAAGCAAG AGCAACAGCT CTGGCTCGGG AAGTGGAACC 
AATTCCAGCA ACTCGAGCAG CTCGCATTGC TACGGCCAGT GTAATTGCAT CATTGACCAG 
ATCTTCACGG GCATGCTGCA GAGCGATGTG GTGTGCCAGG CCTGCAACGG AGTGTCCACC 
ACGTACGATC CGTTTTGGGA CATTTCGCTG GACCTGGGCG AAACAACAAC GCATGGTGGT 
GTTACTCCAA AAACGCTGAT AGACTGCCTG GAGCGGTACA CTCGGGCCGA GCATCTCGGA 
TCGGCGGCCA AAATCAAGTG CTCTACCTGC AAATCCTACC AGGAGTCCAC CAAACAGTTC 
AGCCTGCGCA CTCTGCCCAG CGTGGTGTCG TTCCATTTGA AACGCTTCGA GCACTCAGCT 
CTGATCGATC GCAAGATCTC CTCGTTCATT CAATTCCCCG TGGAGTTCGA CATGACGCCC 
TTTATGTCGG AGAAGAAGAA CGCCTACGGC GACTTCCGGT TCTCTCTGTA CGCCGTGGTC 
AATCATGTGG GCACCATCGA CACCGGCCAC TATACGGCGT ATGTGCGGCA CCAGAAGGAC 
ACGTGGGTCA AGTGCGATGA TCATGTGATA ACGATGGCAT CGCTCAAGCA GGTGCTGGAC 
AGCGAGGGTT ATTTATTGTT CTACCATAAA AATGTGCTGG AATACGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

not ( NM_001300177.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287106.1 Drosophila melanogaster non-stop (not), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa18870
Clone ID Related Accession (Same CDS sequence)	NM_079416.4 , NM_001300177.1
Accession Version	NM_001300177.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	non-stop, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGAGA CGGGTTGTCG GCACTATCAA AGCTATGTGA AGGAGCACAG CTACGATACA 
TTCCGGGTCA TCGACGCCTA CTTCGCTGCT TGCGTCAACA GAGATGCGCG CGAGCGAAAG 
GCTATTCACT GCAACTGCTT CGAGTGCGGC AGCTACGGCA TCCAGTTGTA CGCTTGCCTG 
CACTGCATCT ACTTCGGATG TCGGGGGGCC CACATCACGA GTCACCTGCG ATCCAAGAAG 
CACAATGTGG CCCTGGAGCT GTCCCACGGA ACGCTCTACT GCTACGCCTG CAGAGATTTC 
ATCTACGACG CGCGGAGTCG CGAGTATGCG CTGATCAACC GCAAGCTGGA GGCGAAGGAT 
CTGCAGAAGA GCATCGGCTG GGTGCCGTGG GTTCCCACAA CCAAGGAGAC CAATCTGCTG 
CTGGCCAATG CGAGGCGTCG GCTGGTGAGG CCCAATCAAA CGATCGGACT GAGGGGTCTG 
CTTAATCTGG GCGCCACTTG CTTCATGAAC TGCATCGTCC AGGCTCTGGT TCACACGCCC 
CTGCTCAGCG ATTACTTTAT GTCAGATCGT CACGACTGCG GCAGCAAGTC ATCGCACAAA 
TGTCTCGTTT GTGAAGTGTC ACGTCTCTTC CAGGAGTTCT ATTCCGGCTC CCGTTCGCCG 
CTGTCGCTGC ACCGGCTACT GCATTTAATC TGGAACCATG CGAAGCACTT GGCCGGCTAC 
GAGCAGCAGG ATGCGCACGA GTTCTTCATC GCCACTCTGG ACGTGCTGCA TCGGCACTGC 
GTGAAGGCAA AGGCGGAGCA CGAAAGCAAG AGCAACAGCT CTGGCTCGGG AAGTGGAACC 
AATTCCAGCA ACTCGAGCAG CTCGCATTGC TACGGCCAGT GTAATTGCAT CATTGACCAG 
ATCTTCACGG GCATGCTGCA GAGCGATGTG GTGTGCCAGG CCTGCAACGG AGTGTCCACC 
ACGTACGATC CGTTTTGGGA CATTTCGCTG GACCTGGGCG AAACAACAAC GCATGGTGGT 
GTTACTCCAA AAACGCTGAT AGACTGCCTG GAGCGGTACA CTCGGGCCGA GCATCTCGGA 
TCGGCGGCCA AAATCAAGTG CTCTACCTGC AAATCCTACC AGGAGTCCAC CAAACAGTTC 
AGCCTGCGCA CTCTGCCCAG CGTGGTGTCG TTCCATTTGA AACGCTTCGA GCACTCAGCT 
CTGATCGATC GCAAGATCTC CTCGTTCATT CAATTCCCCG TGGAGTTCGA CATGACGCCC 
TTTATGTCGG AGAAGAAGAA CGCCTACGGC GACTTCCGGT TCTCTCTGTA CGCCGTGGTC 
AATCATGTGG GCACCATCGA CACCGGCCAC TATACGGCGT ATGTGCGGCA CCAGAAGGAC 
ACGTGGGTCA AGTGCGATGA TCATGTGATA ACGATGGCAT CGCTCAAGCA GGTGCTGGAC 
AGCGAGGGTT ATTTATTGTT CTACCATAAA AATGTGCTGG AATACGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287106.1
CDS	639..2129
Translation	MSETGCRHYQSYVKEHSYDTFRVIDAYFAACVNRDARERKAIHCNCFECGSYGIQLYACLHCIYFGCRGAHITSHLRSKKHNVALELSHGTLYCYACRDFIYDARSREYALINRKLEAKDLQKSIGWVPWVPTTKETNLLLANARRRLVRPNQTIGLRGLLNLGATCFMNCIVQALVHTPLLSDYFMSDRHDCGSKSSHKCLVCEVSRLFQEFYSGSRSPLSLHRLLHLIWNHAKHLAGYEQQDAHEFFIATLDVLHRHCVKAKAEHESKSNSSGSGSGTNSSNSSSSHCYGQCNCIIDQIFTGMLQSDVVCQACNGVSTTYDPFWDISLDLGETTTHGGVTPKTLIDCLERYTRAEHLGSAAKIKCSTCKSYQESTKQFSLRTLPSVVSFHLKRFEHSALIDRKISSFIQFPVEFDMTPFMSEKKNAYGDFRFSLYAVVNHVGTIDTGHYTAYVRHQKDTWVKCDDHVITMASLKQVLDSEGYLLFYHKNVLEYE

Target ORF information:

RefSeq Version	NM_001300177.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster non-stop (not), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300177.1

ORF Insert Sequence:

ATGTCCGAGA CGGGTTGTCG GCACTATCAA AGCTATGTGA AGGAGCACAG CTACGATACA 
TTCCGGGTCA TCGACGCCTA CTTCGCTGCT TGCGTCAACA GAGATGCGCG CGAGCGAAAG 
GCTATTCACT GCAACTGCTT CGAGTGCGGC AGCTACGGCA TCCAGTTGTA CGCTTGCCTG 
CACTGCATCT ACTTCGGATG TCGGGGGGCC CACATCACGA GTCACCTGCG ATCCAAGAAG 
CACAATGTGG CCCTGGAGCT GTCCCACGGA ACGCTCTACT GCTACGCCTG CAGAGATTTC 
ATCTACGACG CGCGGAGTCG CGAGTATGCG CTGATCAACC GCAAGCTGGA GGCGAAGGAT 
CTGCAGAAGA GCATCGGCTG GGTGCCGTGG GTTCCCACAA CCAAGGAGAC CAATCTGCTG 
CTGGCCAATG CGAGGCGTCG GCTGGTGAGG CCCAATCAAA CGATCGGACT GAGGGGTCTG 
CTTAATCTGG GCGCCACTTG CTTCATGAAC TGCATCGTCC AGGCTCTGGT TCACACGCCC 
CTGCTCAGCG ATTACTTTAT GTCAGATCGT CACGACTGCG GCAGCAAGTC ATCGCACAAA 
TGTCTCGTTT GTGAAGTGTC ACGTCTCTTC CAGGAGTTCT ATTCCGGCTC CCGTTCGCCG 
CTGTCGCTGC ACCGGCTACT GCATTTAATC TGGAACCATG CGAAGCACTT GGCCGGCTAC 
GAGCAGCAGG ATGCGCACGA GTTCTTCATC GCCACTCTGG ACGTGCTGCA TCGGCACTGC 
GTGAAGGCAA AGGCGGAGCA CGAAAGCAAG AGCAACAGCT CTGGCTCGGG AAGTGGAACC 
AATTCCAGCA ACTCGAGCAG CTCGCATTGC TACGGCCAGT GTAATTGCAT CATTGACCAG 
ATCTTCACGG GCATGCTGCA GAGCGATGTG GTGTGCCAGG CCTGCAACGG AGTGTCCACC 
ACGTACGATC CGTTTTGGGA CATTTCGCTG GACCTGGGCG AAACAACAAC GCATGGTGGT 
GTTACTCCAA AAACGCTGAT AGACTGCCTG GAGCGGTACA CTCGGGCCGA GCATCTCGGA 
TCGGCGGCCA AAATCAAGTG CTCTACCTGC AAATCCTACC AGGAGTCCAC CAAACAGTTC 
AGCCTGCGCA CTCTGCCCAG CGTGGTGTCG TTCCATTTGA AACGCTTCGA GCACTCAGCT 
CTGATCGATC GCAAGATCTC CTCGTTCATT CAATTCCCCG TGGAGTTCGA CATGACGCCC 
TTTATGTCGG AGAAGAAGAA CGCCTACGGC GACTTCCGGT TCTCTCTGTA CGCCGTGGTC 
AATCATGTGG GCACCATCGA CACCGGCCAC TATACGGCGT ATGTGCGGCA CCAGAAGGAC 
ACGTGGGTCA AGTGCGATGA TCATGTGATA ACGATGGCAT CGCTCAAGCA GGTGCTGGAC 
AGCGAGGGTT ATTTATTGTT CTACCATAAA AATGTGCTGG AATACGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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not cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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