Prestin cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prestin
Entrez Gene ID	39996
Full Name	CG5485 gene product from transcript CG5485-RA
Synonyms	CG5485,CG5845,Dmel\CG5485,Slc26a5,dPrestin,dpres
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 75A8-75A8

mRNA and Protein(s)

mRNA	Protein	Name
NM_140767.2	NP_649024.1	prestin

Pathways from BioSystems

Homologies

Homo sapiens (human)	SLC26A5	NP_945350.1
Canis lupus familiaris (dog)	SLC26A5	XP_540393.2
Drosophila melanogaster (fruit fly)	Prestin	NP_649024.1
Caenorhabditis elegans (roundworm)	sulp-7	NP_001033571.1
Caenorhabditis elegans (roundworm)	sulp-4	NP_505989.1
Caenorhabditis elegans (roundworm)	sulp-3	NP_509424.2
Rattus norvegicus (Norway rat)	Slc26a5	NP_110467.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010389	XP_559067.3
Caenorhabditis elegans (roundworm)	sulp-8	NP_001256129.1
Xenopus tropicalis (tropical clawed frog)	slc26a5	XP_002933151.2
Gallus gallus (chicken)	SLC26A5	NP_001072945.1
Oryza sativa (rice)	Os04g0652400	NP_001054098.1
Pan troglodytes (chimpanzee)	SLC26A5	XP_003339466.1
Bos taurus (cattle)	SLC26A5	NP_001179807.1
Mus musculus (house mouse)	Slc26a5	NP_109652.3
Danio rerio (zebrafish)	slc26a5	NP_958881.1
Caenorhabditis elegans (roundworm)	sulp-5	NP_505990.2
Arabidopsis thaliana (thale cress)	AST91	NP_173722.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results demonstrate that prestin is expressed in Johnston's organ neurons yet is not required for auditory amplification in D. melanogaster
Title: Prestin is an anion transporter dispensable for mechanical feedback amplification in Drosophila hearing.

appears suited for central roles in bicarbonate, sulfate and oxalate metabolism including generating the high pH conditions measured in the Dipteran midgut lumen
Title: Ion and solute transport by Prestin in Drosophila and Anopheles.

The following Prestin gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prestin cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa21049	NM_140767.2 Latest version!	Drosophila melanogaster prestin (Prestin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prestin ( NM_140767.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649024.1 Drosophila melanogaster prestin (Prestin), mRNA.

Price & Availability↑

CloneID	OFa21049
Clone ID Related Accession (Same CDS sequence)	NM_140767.2
Accession Version	NM_140767.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	prestin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_140767.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCTAGTG ATAAAGAAAG TCCAGAAACC AAGCCACTGA ACAATAACAA TACAAATGGA 
AACGGTGGCA GTGTACCAAG CAAGCCAAAG ATCCAGCCAA AATATAGCAT TCACCGAGAT 
GTGCTGACCC ATGAGGTGGT GATAAAGCAA ACAGGATATG CGGCCAGGGA CAAGAGCATA 
CCCTCCTCGC TGCGAAACTG CTGGCGGAGC TGGAACTTCT TTGCACTCTT CACCGGCGTG 
ATTCCCATTC TGCAATGGCT ACCCCAGTAT TCACCCAGGA GAGATCTTCC TGGCGATATA 
ATCGCCGGCT TCACCGTCGC CATCATGAAC ATACCTCATG GCATGGCTTA TGGCATTCTA 
GCAGGAGTTT CGGCTGGAAA TGGTCTCTAT ATGGCCGTGT TTCCGGTGCT GGCCTACATG 
TTCCTGGGGA CCTCCAAGCA CATCTCCATC GGCACTTTTG CGGTGGCCAG CATGATGACG 
GCAAAGGTGG TGGATACGTA TGCCAACGTA GATGACCACC ATCAGATATT GCCTATTAAC 
GCCTTCGGTT TGCAATCGAA TGGAACAGCG ACTGCGTCCC CACTCCTGCT AATAAATTCC 
TCAGCGCTTG CGGATTCTGT CTCCTCAACC ACTATATCAC CTTTTCAGCT TTTAAATTCT 
ACTCTAAATG CGGATCCCAT TACTAAAATA GAGGTGGCGA CCTCTTTGGC ACTTACCGTG 
GGAATTGTAA ACCTCCTAAT GGCCTTTCTA AGACTGGGAA CCCTGTCATC CCTTTTGAGT 
GAACCTCTGG TGAATGGATT CACCACTGCT GCAGCGTGTC ACGTGGTTAC CGCCCAGCTG 
AAGGATGTGC TGGGCATCTC AGTACCGCGT CACAAAGGTG CATTCAAGAT CATATATACC 
GTTATTGACG TGATCAAGGG AGTGCCACAA ACGAATCTTG TGAACTTTGG CTTTTGTATG 
GCTGTGATTG CGTTCATGAT GATCTGCAAT GAGATCCTAA AGCCGCGACT GAGTAAAAAG 
TGTCGCTTTC CCCTGCCGGC GGAGCTGATC ATGGTGATCG GTGGTACTTT AATCTCCAAA 
TGGTTCAACC TGTATGTCGA TTACAACGTA AATCCAGTGG GAAAAATACC CAGTGGCTTA 
CCGGAGCCAG TTCTTCCGCG CTTGGATCTT GTACCAAAGG TGGCCGTAGA TTCAATTGCC 
ATTGCTATCG TCACCTACTC CATAATCATG TCCATGGGCC TAACGTTCGC TAAGAAGCAC 
GGATATGAGG TCAGACCAAA TCAGGAGCTG TTCGCCATGG GCATTGGAAA CATGGTTGGC 
GGCTGTTTCT CCTGCATTCC GATGGCGTGC TCTCTGTCAA GATCGGTAAT TCAGGATCAG 
ACTGGTGGGG TTTCGCAGAT AGCTTCATTG GTCTCCGCTT CGCTGGTGGT GGTCACCCTA 
ATGTGGATCG GCCCCTTTTT CAGCAGTTTG CCAAGGTGCG TTCTGGCTGG CGTGATTATA 
GTGGCACTGA AGCCGATGTT TATGCAGGCC AAGGAATTGA AAAAGTTCTC CAAGCAGGGC 
AAGTTGGAAA TGTTCACATG GATATCTACC TTCCTCTGCG TCGTTATCAT CGATATTGAT 
ATTGGGCTTT TGATTGGCAT CTGCATCTCC TTGTTGGCTC TGTATATCAA GGGACTGAAG 
CCGTACTCCT GCCTTTTGGG CTATATGCCA GAGGCTCCAG GCATCTATAT GGATCTCAAC 
CAGCATCGAA ACGCCATGCA GGTTCCCGAA GTCCGCATCT TCCGCTACAG TGGATCGCTT 
AACTTTGCCA CCAGCCTGTT CTTCCGACGT GCTCTGTACG AGGCCGTGGG CCTGGACAAG 
ATTCCCCTAA CGAAAGTCAG CAGCAGCAAT AGCAATAGTC CCAGCAAGGG CAGCAAATCG 
AGTTATTCCC CAGTATCGCA GAACGGAGGC AAGGCGATCA ATGGCAAGTT AGAAGAGACG 
TCAGGTGCTT TCAAGGTGCT CGTCCTGGAT TTCTCCATGC TGGGACACAT CGATGTGGCT 
GGATGTCGCA CCCTGACGGA TCTGAGCAAG GAACTGAAGG TGCGAGGAGC TCGTTTGTTG 
TTGGCTAGCC CCGTAGATCG GGTATACGAT ACCCTCGTGC ACAGTATGGC CCTTAGCGAA 
GGACCTTTTG AGATCTTCCC CACCCTGCAC GATTGTGTGG AATATGCCAA TGCCTGTCGA 
ACAGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649024.1
CDS	945..3173
Translation	MPSDKESPETKPLNNNNTNGNGGSVPSKPKIQPKYSIHRDVLTHEVVIKQTGYAARDKSIPSSLRNCWRSWNFFALFTGVIPILQWLPQYSPRRDLPGDIIAGFTVAIMNIPHGMAYGILAGVSAGNGLYMAVFPVLAYMFLGTSKHISIGTFAVASMMTAKVVDTYANVDDHHQILPINAFGLQSNGTATASPLLLINSSALADSVSSTTISPFQLLNSTLNADPITKIEVATSLALTVGIVNLLMAFLRLGTLSSLLSEPLVNGFTTAAACHVVTAQLKDVLGISVPRHKGAFKIIYTVIDVIKGVPQTNLVNFGFCMAVIAFMMICNEILKPRLSKKCRFPLPAELIMVIGGTLISKWFNLYVDYNVNPVGKIPSGLPEPVLPRLDLVPKVAVDSIAIAIVTYSIIMSMGLTFAKKHGYEVRPNQELFAMGIGNMVGGCFSCIPMACSLSRSVIQDQTGGVSQIASLVSASLVVVTLMWIGPFFSSLPRCVLAGVIIVALKPMFMQAKELKKFSKQGKLEMFTWISTFLCVVIIDIDIGLLIGICISLLALYIKGLKPYSCLLGYMPEAPGIYMDLNQHRNAMQVPEVRIFRYSGSLNFATSLFFRRALYEAVGLDKIPLTKVSSSNSNSPSKGSKSSYSPVSQNGGKAINGKLEETSGAFKVLVLDFSMLGHIDVAGCRTLTDLSKELKVRGARLLLASPVDRVYDTLVHSMALSEGPFEIFPTLHDCVEYANACRTA

Target ORF information:

RefSeq Version	NM_140767.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster prestin (Prestin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140767.2

ORF Insert Sequence:

ATGCCTAGTG ATAAAGAAAG TCCAGAAACC AAGCCACTGA ACAATAACAA TACAAATGGA 
AACGGTGGCA GTGTACCAAG CAAGCCAAAG ATCCAGCCAA AATATAGCAT TCACCGAGAT 
GTGCTGACCC ATGAGGTGGT GATAAAGCAA ACAGGATATG CGGCCAGGGA CAAGAGCATA 
CCCTCCTCGC TGCGAAACTG CTGGCGGAGC TGGAACTTCT TTGCACTCTT CACCGGCGTG 
ATTCCCATTC TGCAATGGCT ACCCCAGTAT TCACCCAGGA GAGATCTTCC TGGCGATATA 
ATCGCCGGCT TCACCGTCGC CATCATGAAC ATACCTCATG GCATGGCTTA TGGCATTCTA 
GCAGGAGTTT CGGCTGGAAA TGGTCTCTAT ATGGCCGTGT TTCCGGTGCT GGCCTACATG 
TTCCTGGGGA CCTCCAAGCA CATCTCCATC GGCACTTTTG CGGTGGCCAG CATGATGACG 
GCAAAGGTGG TGGATACGTA TGCCAACGTA GATGACCACC ATCAGATATT GCCTATTAAC 
GCCTTCGGTT TGCAATCGAA TGGAACAGCG ACTGCGTCCC CACTCCTGCT AATAAATTCC 
TCAGCGCTTG CGGATTCTGT CTCCTCAACC ACTATATCAC CTTTTCAGCT TTTAAATTCT 
ACTCTAAATG CGGATCCCAT TACTAAAATA GAGGTGGCGA CCTCTTTGGC ACTTACCGTG 
GGAATTGTAA ACCTCCTAAT GGCCTTTCTA AGACTGGGAA CCCTGTCATC CCTTTTGAGT 
GAACCTCTGG TGAATGGATT CACCACTGCT GCAGCGTGTC ACGTGGTTAC CGCCCAGCTG 
AAGGATGTGC TGGGCATCTC AGTACCGCGT CACAAAGGTG CATTCAAGAT CATATATACC 
GTTATTGACG TGATCAAGGG AGTGCCACAA ACGAATCTTG TGAACTTTGG CTTTTGTATG 
GCTGTGATTG CGTTCATGAT GATCTGCAAT GAGATCCTAA AGCCGCGACT GAGTAAAAAG 
TGTCGCTTTC CCCTGCCGGC GGAGCTGATC ATGGTGATCG GTGGTACTTT AATCTCCAAA 
TGGTTCAACC TGTATGTCGA TTACAACGTA AATCCAGTGG GAAAAATACC CAGTGGCTTA 
CCGGAGCCAG TTCTTCCGCG CTTGGATCTT GTACCAAAGG TGGCCGTAGA TTCAATTGCC 
ATTGCTATCG TCACCTACTC CATAATCATG TCCATGGGCC TAACGTTCGC TAAGAAGCAC 
GGATATGAGG TCAGACCAAA TCAGGAGCTG TTCGCCATGG GCATTGGAAA CATGGTTGGC 
GGCTGTTTCT CCTGCATTCC GATGGCGTGC TCTCTGTCAA GATCGGTAAT TCAGGATCAG 
ACTGGTGGGG TTTCGCAGAT AGCTTCATTG GTCTCCGCTT CGCTGGTGGT GGTCACCCTA 
ATGTGGATCG GCCCCTTTTT CAGCAGTTTG CCAAGGTGCG TTCTGGCTGG CGTGATTATA 
GTGGCACTGA AGCCGATGTT TATGCAGGCC AAGGAATTGA AAAAGTTCTC CAAGCAGGGC 
AAGTTGGAAA TGTTCACATG GATATCTACC TTCCTCTGCG TCGTTATCAT CGATATTGAT 
ATTGGGCTTT TGATTGGCAT CTGCATCTCC TTGTTGGCTC TGTATATCAA GGGACTGAAG 
CCGTACTCCT GCCTTTTGGG CTATATGCCA GAGGCTCCAG GCATCTATAT GGATCTCAAC 
CAGCATCGAA ACGCCATGCA GGTTCCCGAA GTCCGCATCT TCCGCTACAG TGGATCGCTT 
AACTTTGCCA CCAGCCTGTT CTTCCGACGT GCTCTGTACG AGGCCGTGGG CCTGGACAAG 
ATTCCCCTAA CGAAAGTCAG CAGCAGCAAT AGCAATAGTC CCAGCAAGGG CAGCAAATCG 
AGTTATTCCC CAGTATCGCA GAACGGAGGC AAGGCGATCA ATGGCAAGTT AGAAGAGACG 
TCAGGTGCTT TCAAGGTGCT CGTCCTGGAT TTCTCCATGC TGGGACACAT CGATGTGGCT 
GGATGTCGCA CCCTGACGGA TCTGAGCAAG GAACTGAAGG TGCGAGGAGC TCGTTTGTTG 
TTGGCTAGCC CCGTAGATCG GGTATACGAT ACCCTCGTGC ACAGTATGGC CCTTAGCGAA 
GGACCTTTTG AGATCTTCCC CACCCTGCAC GATTGTGTGG AATATGCCAA TGCCTGTCGA 
ACAGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Prestin cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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