MED19 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED19
Entrez Gene ID	39987
Full Name	Mediator complex subunit 19
Synonyms	CG5546,Dmel\CG5546,Med19
General protein information	Preferred Names Mediator complex subunit 19 Names CG5546 gene product from transcript CG5546-RB CG5546-PA CG5546-PB MED19-PA MED19-PB mediator complex subunit 19
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 75A4-75A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_140759.3	NP_649016.1	mediator complex subunit 19, isoform A
NM_001300174.1	NP_001287103.1	mediator complex subunit 19, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	MED19	NP_649016.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011626	XP_320879.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

supports a role for Med19 as a PolII holoenzyme-embedded "co-factor" that acts together with Hox proteins through their homeodomains in regulated developmental transcription
Title: Drosophila melanogaster Hox transcription factors access the RNA polymerase II machinery through direct homeodomain binding to a conserved motif of mediator subunit Med19.

The following MED19 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED19 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa21042	NM_001300174.1 Latest version!	Drosophila melanogaster mediator complex subunit 19, transcript variant B (MED19), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa21042	NM_140759.3 Latest version!	Drosophila melanogaster mediator complex subunit 19, transcript variant A (MED19), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED19 ( NM_140759.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649016.1 Drosophila melanogaster mediator complex subunit 19, transcript variant A (MED19), mRNA.

Price & Availability↑

CloneID	OFa21042
Clone ID Related Accession (Same CDS sequence)	NM_140759.3 , NM_001300174.1
Accession Version	NM_140759.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mediator complex subunit 19, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140759.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGCA ACTACGGCAA CATGATGGAC TCACCGAAGT CATCGCCCCA CGGCGGCGGC 
CGATCACCCG TGGTGGCCCG TCAAGACTCA TCCGGCACCC TGAAAACGAC CATTTCGCTG 
GGCAAGACGC CAACGATCAT TCACACGGGG CCATTTTACT CCATGAAGGA ACCACCCGCC 
AAGGCAGAAC TTACCGGCGA CAAGGATCTC ATGACCGAGT ACGGACTGCA CCACACACTC 
ACCAAGTTCA AGGAGAAGAA GTTCAAGGAG TCGCTGGCCT CGTTTCTGCA GAACATCCCC 
GGCATTAACG ACCTCATCAC GCATCCCGTG GAGAATAGCA CGCTGCGGAG CGTGATCGAA 
AAGCCGCCCA TTGGTGGAAA GGAGCTTCTT CCGCTGACTC CCGTGCAGCT GGCCGGATTC 
CGCTTGCATC CCGGACCGCT GCCGGAACAG TATCGTACCA CATATGTCAC ACCTGCGCGT 
AAACACAAAA ACAAACACAA AAAGCAAAAA CACAAAGACG GGGTGACCAC GGGACAGGAA 
TCCACGCTGC TCGACTCGGC CGGCCTGGAG ACTTACGAGA AAAAGCACAA GAAGCAGAAA 
CGCCATGAGG ACGACAAGGA GCGCAAGAAG CGCAAAAAGG AGAAAAAGCG TAAAAAGAAA 
AACCAGAGCC CCGAACCGGG CGTGGGCTTG CTGCCCGGAG GAGCGAGCTT GGGCGGAGCA 
GCGGGTGTAA TGGGAGCCAC TAGTCTGGGA TCGCTGGGAG GAGGACCTGG AGGACCCGGG 
CTGTCCAGCC TTAGTGCCAG TATGGGACCT GGCGTTGTGG GGAACATGAA TAGCTTCTCC 
TCATCGATGC AGATGCAGCA GCAACAGCAG ATGCCCATGC AGCAACAGCA GATGTCAAGT 
GGCGGTCTGC TGGGATCTGT GCTGGGCACT AGTGGCGGTC CAGGTGGCGG CGGCGGAGGA 
GGAGGTGGTG GCGGGGGCGG CGTCGGTGGG AGTCTGCTTA TGTCGCAGTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649016.1
CDS	311..1324
Translation	MMSNYGNMMDSPKSSPHGGGRSPVVARQDSSGTLKTTISLGKTPTIIHTGPFYSMKEPPAKAELTGDKDLMTEYGLHHTLTKFKEKKFKESLASFLQNIPGINDLITHPVENSTLRSVIEKPPIGGKELLPLTPVQLAGFRLHPGPLPEQYRTTYVTPARKHKNKHKKQKHKDGVTTGQESTLLDSAGLETYEKKHKKQKRHEDDKERKKRKKEKKRKKKNQSPEPGVGLLPGGASLGGAAGVMGATSLGSLGGGPGGPGLSSLSASMGPGVVGNMNSFSSSMQMQQQQQMPMQQQQMSSGGLLGSVLGTSGGPGGGGGGGGGGGGGVGGSLLMSQF

Target ORF information:

RefSeq Version	NM_140759.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mediator complex subunit 19, transcript variant A (MED19), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140759.3

ORF Insert Sequence:

ATGATGAGCA ACTACGGCAA CATGATGGAC TCACCGAAGT CATCGCCCCA CGGCGGCGGC 
CGATCACCCG TGGTGGCCCG TCAAGACTCA TCCGGCACCC TGAAAACGAC CATTTCGCTG 
GGCAAGACGC CAACGATCAT TCACACGGGG CCATTTTACT CCATGAAGGA ACCACCCGCC 
AAGGCAGAAC TTACCGGCGA CAAGGATCTC ATGACCGAGT ACGGACTGCA CCACACACTC 
ACCAAGTTCA AGGAGAAGAA GTTCAAGGAG TCGCTGGCCT CGTTTCTGCA GAACATCCCC 
GGCATTAACG ACCTCATCAC GCATCCCGTG GAGAATAGCA CGCTGCGGAG CGTGATCGAA 
AAGCCGCCCA TTGGTGGAAA GGAGCTTCTT CCGCTGACTC CCGTGCAGCT GGCCGGATTC 
CGCTTGCATC CCGGACCGCT GCCGGAACAG TATCGTACCA CATATGTCAC ACCTGCGCGT 
AAACACAAAA ACAAACACAA AAAGCAAAAA CACAAAGACG GGGTGACCAC GGGACAGGAA 
TCCACGCTGC TCGACTCGGC CGGCCTGGAG ACTTACGAGA AAAAGCACAA GAAGCAGAAA 
CGCCATGAGG ACGACAAGGA GCGCAAGAAG CGCAAAAAGG AGAAAAAGCG TAAAAAGAAA 
AACCAGAGCC CCGAACCGGG CGTGGGCTTG CTGCCCGGAG GAGCGAGCTT GGGCGGAGCA 
GCGGGTGTAA TGGGAGCCAC TAGTCTGGGA TCGCTGGGAG GAGGACCTGG AGGACCCGGG 
CTGTCCAGCC TTAGTGCCAG TATGGGACCT GGCGTTGTGG GGAACATGAA TAGCTTCTCC 
TCATCGATGC AGATGCAGCA GCAACAGCAG ATGCCCATGC AGCAACAGCA GATGTCAAGT 
GGCGGTCTGC TGGGATCTGT GCTGGGCACT AGTGGCGGTC CAGGTGGCGG CGGCGGAGGA 
GGAGGTGGTG GCGGGGGCGG CGTCGGTGGG AGTCTGCTTA TGTCGCAGTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED19 ( NM_001300174.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287103.1 Drosophila melanogaster mediator complex subunit 19, transcript variant B (MED19), mRNA.

Price & Availability↑

CloneID	OFa21042
Clone ID Related Accession (Same CDS sequence)	NM_140759.3 , NM_001300174.1
Accession Version	NM_001300174.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mediator complex subunit 19, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGCA ACTACGGCAA CATGATGGAC TCACCGAAGT CATCGCCCCA CGGCGGCGGC 
CGATCACCCG TGGTGGCCCG TCAAGACTCA TCCGGCACCC TGAAAACGAC CATTTCGCTG 
GGCAAGACGC CAACGATCAT TCACACGGGG CCATTTTACT CCATGAAGGA ACCACCCGCC 
AAGGCAGAAC TTACCGGCGA CAAGGATCTC ATGACCGAGT ACGGACTGCA CCACACACTC 
ACCAAGTTCA AGGAGAAGAA GTTCAAGGAG TCGCTGGCCT CGTTTCTGCA GAACATCCCC 
GGCATTAACG ACCTCATCAC GCATCCCGTG GAGAATAGCA CGCTGCGGAG CGTGATCGAA 
AAGCCGCCCA TTGGTGGAAA GGAGCTTCTT CCGCTGACTC CCGTGCAGCT GGCCGGATTC 
CGCTTGCATC CCGGACCGCT GCCGGAACAG TATCGTACCA CATATGTCAC ACCTGCGCGT 
AAACACAAAA ACAAACACAA AAAGCAAAAA CACAAAGACG GGGTGACCAC GGGACAGGAA 
TCCACGCTGC TCGACTCGGC CGGCCTGGAG ACTTACGAGA AAAAGCACAA GAAGCAGAAA 
CGCCATGAGG ACGACAAGGA GCGCAAGAAG CGCAAAAAGG AGAAAAAGCG TAAAAAGAAA 
AACCAGAGCC CCGAACCGGG CGTGGGCTTG CTGCCCGGAG GAGCGAGCTT GGGCGGAGCA 
GCGGGTGTAA TGGGAGCCAC TAGTCTGGGA TCGCTGGGAG GAGGACCTGG AGGACCCGGG 
CTGTCCAGCC TTAGTGCCAG TATGGGACCT GGCGTTGTGG GGAACATGAA TAGCTTCTCC 
TCATCGATGC AGATGCAGCA GCAACAGCAG ATGCCCATGC AGCAACAGCA GATGTCAAGT 
GGCGGTCTGC TGGGATCTGT GCTGGGCACT AGTGGCGGTC CAGGTGGCGG CGGCGGAGGA 
GGAGGTGGTG GCGGGGGCGG CGTCGGTGGG AGTCTGCTTA TGTCGCAGTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287103.1
CDS	311..1324
Translation	MMSNYGNMMDSPKSSPHGGGRSPVVARQDSSGTLKTTISLGKTPTIIHTGPFYSMKEPPAKAELTGDKDLMTEYGLHHTLTKFKEKKFKESLASFLQNIPGINDLITHPVENSTLRSVIEKPPIGGKELLPLTPVQLAGFRLHPGPLPEQYRTTYVTPARKHKNKHKKQKHKDGVTTGQESTLLDSAGLETYEKKHKKQKRHEDDKERKKRKKEKKRKKKNQSPEPGVGLLPGGASLGGAAGVMGATSLGSLGGGPGGPGLSSLSASMGPGVVGNMNSFSSSMQMQQQQQMPMQQQQMSSGGLLGSVLGTSGGPGGGGGGGGGGGGGVGGSLLMSQF

Target ORF information:

RefSeq Version	NM_001300174.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mediator complex subunit 19, transcript variant B (MED19), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300174.1

ORF Insert Sequence:

ATGATGAGCA ACTACGGCAA CATGATGGAC TCACCGAAGT CATCGCCCCA CGGCGGCGGC 
CGATCACCCG TGGTGGCCCG TCAAGACTCA TCCGGCACCC TGAAAACGAC CATTTCGCTG 
GGCAAGACGC CAACGATCAT TCACACGGGG CCATTTTACT CCATGAAGGA ACCACCCGCC 
AAGGCAGAAC TTACCGGCGA CAAGGATCTC ATGACCGAGT ACGGACTGCA CCACACACTC 
ACCAAGTTCA AGGAGAAGAA GTTCAAGGAG TCGCTGGCCT CGTTTCTGCA GAACATCCCC 
GGCATTAACG ACCTCATCAC GCATCCCGTG GAGAATAGCA CGCTGCGGAG CGTGATCGAA 
AAGCCGCCCA TTGGTGGAAA GGAGCTTCTT CCGCTGACTC CCGTGCAGCT GGCCGGATTC 
CGCTTGCATC CCGGACCGCT GCCGGAACAG TATCGTACCA CATATGTCAC ACCTGCGCGT 
AAACACAAAA ACAAACACAA AAAGCAAAAA CACAAAGACG GGGTGACCAC GGGACAGGAA 
TCCACGCTGC TCGACTCGGC CGGCCTGGAG ACTTACGAGA AAAAGCACAA GAAGCAGAAA 
CGCCATGAGG ACGACAAGGA GCGCAAGAAG CGCAAAAAGG AGAAAAAGCG TAAAAAGAAA 
AACCAGAGCC CCGAACCGGG CGTGGGCTTG CTGCCCGGAG GAGCGAGCTT GGGCGGAGCA 
GCGGGTGTAA TGGGAGCCAC TAGTCTGGGA TCGCTGGGAG GAGGACCTGG AGGACCCGGG 
CTGTCCAGCC TTAGTGCCAG TATGGGACCT GGCGTTGTGG GGAACATGAA TAGCTTCTCC 
TCATCGATGC AGATGCAGCA GCAACAGCAG ATGCCCATGC AGCAACAGCA GATGTCAAGT 
GGCGGTCTGC TGGGATCTGT GCTGGGCACT AGTGGCGGTC CAGGTGGCGG CGGCGGAGGA 
GGAGGTGGTG GCGGGGGCGG CGTCGGTGGG AGTCTGCTTA TGTCGCAGTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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MED19 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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