anchor cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	anchor
Entrez Gene ID	39965
Full Name	CG7510 gene product from transcript CG7510-RB
Synonyms	Dmel\CG7510,anon-74EFa
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 74E2-74E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_140741.3	NP_648998.2	anchor, isoform A
NM_001275066.1	NP_001261995.1	anchor, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GPR155	XP_003309468.1
	GPR155	XP_001089835.2
Gallus gallus (chicken)	GPR155	XP_004942786.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006992	XP_308778.4
Xenopus tropicalis (tropical clawed frog)	gpr155	XP_002934661.2
Homo sapiens (human)	GPR155	NP_001253979.1
Bos taurus (cattle)	GPR155	XP_005202441.1
Mus musculus (house mouse)	Gpr155	NP_001177226.1
Canis lupus familiaris (dog)	GPR155	XP_005640386.1
Rattus norvegicus (Norway rat)	Gpr155	NP_001101281.1
Danio rerio (zebrafish)	gpr155a	XP_005172576.1
Drosophila melanogaster (fruit fly)	CG7510	NP_648998.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following anchor gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the anchor cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18841	NM_001275066.1 Latest version!	Drosophila melanogaster anchor, transcript variant B (anchor), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa21024	NM_140741.3 Latest version!	Drosophila melanogaster anchor, transcript variant A (anchor), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

anchor ( NM_001275066.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261995.1 Drosophila melanogaster anchor, transcript variant B (anchor), mRNA.

Price & Availability↑

CloneID	OFa18841
Clone ID Related Accession (Same CDS sequence)	NM_001275066.1
Accession Version	NM_001275066.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	anchor, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAGCT CCATGTACTA CGCCAGCCAG CGGATTGCTG CCAGCACAGA GGCTAATGTC 
AGAGTCATCA CCTCGACCAT GGCCTCATCT TTGGCCAATG GAACGGCGAC GCCACGCGCC 
GACGACGGGG GCGTATCCAT GAACAACTTT TACCCGGCGT TGGTTCAGTG TTTCGGCATC 
ATCATTTGCG GTTACATTGC CGGACGCTTC AAGATCATCA GCAATGCGGA AACCAAGGGT 
CTGGGCACGT TTGTGGGCAC CTTCGCCCTG CCCTCGCTGA TCTTCCTGTC CCTGGTCGAG 
CTCAACTGGA GCGCTGTCAA CTGGAGCTTC CTTCTGGCCA TGCTCGTCTC CAAGGCGGTG 
GTCTTCTTCG CCGTGCTGAT CATCTCGCTG CTGGTCGCCA GGCCCCTGAA CTACGCTCGC 
GGCGGCCTGA TGGCAATATT CTGCACCCAG AGCAATGACT TCGCTATCGG CTATCCCATA 
GTTATGGCTC TTTATAAGGA TGTCCATCCG GAATATGCCT CGTATCTGTA CCTGATGGCT 
CCTATCTCAC TGGCTATTCT AAATCCAGTT GGTCTGGTCC TCATGGAGAT CTCCAAGATA 
ATCAAGAACA AGGAGGATGT GACCCGCAAT CCACCATTGT GTCCAGAAAC TTGTCCCGCG 
GAACAGATGT CCAAACGGAA TCGCTGCCTG GGTGAACGGT CTATACTGGT TTTCAACACC 
CTAATTGCAC TGTTCTTTAA CCCGTTGCTG CTGATGACCT TGCTGGGTGT TGCAGGAGGA 
TTCCTGTTTC CCAACGGTCT GCCCGAAATG GTGTCCAGTA CGTTGCGTGT TCTTGGCCAG 
AGCTTTTCGG CAACGGCCTT GTTCCTACTC GGTCTTAAGA TCGTAGGTGG GACAGGATCC 
GAACGAAAGA GTATAGGCTT TCTATTGCCT GGTGTTCTTA TACTGGTTAA AATATTGGTG 
CTACCGCTCG TAATCCGACA GACGGTAAAC ATTATGCAGT CTGGCCAGAA CTTCAACGAC 
ACCACCGAGC TGAGCACTTT TGGCTTTCTC TACGGCACCT TTCCAGCTGC CCCCGGGGCT 
TTCGTCATTG CGACACAGTA CAACATGGAG GTTGAATTGG TTGCTCGAAG CATGGTTTTC 
TGTACATTCA TCTCGGCACC GCTGATGTTT ATATCAGCCA AAATGATATC GCTGACGAAC 
CTGAAGCCAT TGGACTATCT CCATGAGCTG GATGCCTTTT CGTTTGACAT CAGCGTGGCC 
GGCGCGGCTG CCTGTTGTAT CATGCTGGCA TTGTTGATCG TGACGAAGCG CTTCAAGCGT 
ATGCCCCAAA GAATAACTTT CTGTCTGGTG CTTTCTCAGC TCATGTGCTG CATAGGAGTA 
ATCCTCTGGT CCAAGATGGA GCACGTGCAC CGTTGGCCTT TGTACCTGCA GTTTCTTCTA 
TTTAATATCG GAACATACAG CACTCGTCTG TGGACTGGTC TTTTGGCCAT TTCATTGCTC 
TTCCTGCAGT GTCGCAGCTT GTGCTTTGTG CTGAAACTAT GGCCCTATAT GTTGGTGTTT 
GCCTGGGGTG TGCCGGCCGT ATTATCTGCC CTTTTGATTG CCTTCGACTC GAATGTGATG 
TCGGGAGAGA AACACAATCC GAGCTTTCAG TACGGAAATG CTCAGGCAGC CATCTCGGTG 
TTTATGCTCG TTATGTGCTT TATAGTAACC GTTGGCTGTT TGGTGCTGCA TCAGCGTTAT 
AAGAAACGAT ACGAAAAGTA CATGACCTAT TCACGTGAGT TGTCCAATCC GGAGTCGGAA 
TCATCCGATC TGCACTCGAC CATATCAACG ACTAATCTAT TGAGTCACAC GGATCAATCG 
TCCATCAGGC AAAGCGTTCG CACAGCTTCT TATTCGTCTT CATCCGACGA TGAGGAAATG 
ATACCAACAG CTGCGGGACT GCCAACCAGT AGGTCAAATG GTGCTGCCAG TGGAGGAGCA 
GGATCAGGCG GAGGAGGTTG TGGTTCTGGC AATGGAACTT GCTGTTCGGG CGGCGCGCCT 
GTGAGCAATT CCTCAACAAC CAGCACCGTG GTTGTGGACA TTGAGGATCT GTTGAATCGC 
ACACGCCAAC GAGCCAGTGG TGCCAACAAT AAAGATCTAA ACAAGATCGA AAGTGCACCT 
GCCACGGAAG AAATTCGCAA TCAAATGTGC AGCAGCTCCT TTAACTGTGG CCCAAGTACC 
TCACGTCAAA GTTGTCAGAC TATTATCGAG CGATATGAGG ATCAGAACAG AAGGGGACTT 
GAGCCCCTGG AACACCCGAG CGACGCTGAT GAGCACCAAA CTTTGAAGCA TACCGTGCTC 
TTGATCACTT TGCTGTGCTC CATGTTTGTC GGTCTGGCAG TGTCCATTTG GACATTGGTC 
ATGGAGGGAA TGTCGGGCAT ATATCTGGAA CTAAGTTTCC TCGATGCTTT CCTCAACTTT 
GGTCAAGGTC TGATTGTTTT GGCAGTGTTT ATCACCGACA TTGGCGAACT TCTAATGCCA 
GTTGTGAAAC TATGGCGCAA GTTATGGTAT GGAGCCAATG TGGTCAGCCT ACCGCACTGG 
TCAAACGTGC GGCCCGAAAC CAAGCATATT TGTGAACAAT TCCGTAATCA TCATTTGGAG 
AACTGCAAAA AGGATATTGC AAAGGACCGA AGGTGGCGTA TTCGTGTGTA TCGCAGAGTG 
TTCTACGGCA CCGAGTTCGT CAGCTGGCTC ATCGAGGTGG GACTGTCCAA GGATCGAATG 
GAGGCTGTGC ATTATGCCCG ACATCTGGTC GATGGCCGGG TTTTGCGACA CATAAACAAC 
GTTTATCACT TTGAGGATAA GCTGCTGCTC TACAATTTCT GCAGTCGCAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261995.1
CDS	480..3353
Translation	MDSSMYYASQRIAASTEANVRVITSTMASSLANGTATPRADDGGVSMNNFYPALVQCFGIIICGYIAGRFKIISNAETKGLGTFVGTFALPSLIFLSLVELNWSAVNWSFLLAMLVSKAVVFFAVLIISLLVARPLNYARGGLMAIFCTQSNDFAIGYPIVMALYKDVHPEYASYLYLMAPISLAILNPVGLVLMEISKIIKNKEDVTRNPPLCPETCPAEQMSKRNRCLGERSILVFNTLIALFFNPLLLMTLLGVAGGFLFPNGLPEMVSSTLRVLGQSFSATALFLLGLKIVGGTGSERKSIGFLLPGVLILVKILVLPLVIRQTVNIMQSGQNFNDTTELSTFGFLYGTFPAAPGAFVIATQYNMEVELVARSMVFCTFISAPLMFISAKMISLTNLKPLDYLHELDAFSFDISVAGAAACCIMLALLIVTKRFKRMPQRITFCLVLSQLMCCIGVILWSKMEHVHRWPLYLQFLLFNIGTYSTRLWTGLLAISLLFLQCRSLCFVLKLWPYMLVFAWGVPAVLSALLIAFDSNVMSGEKHNPSFQYGNAQAAISVFMLVMCFIVTVGCLVLHQRYKKRYEKYMTYSRELSNPESESSDLHSTISTTNLLSHTDQSSIRQSVRTASYSSSSDDEEMIPTAAGLPTSRSNGAASGGAGSGGGGCGSGNGTCCSGGAPVSNSSTTSTVVVDIEDLLNRTRQRASGANNKDLNKIESAPATEEIRNQMCSSSFNCGPSTSRQSCQTIIERYEDQNRRGLEPLEHPSDADEHQTLKHTVLLITLLCSMFVGLAVSIWTLVMEGMSGIYLELSFLDAFLNFGQGLIVLAVFITDIGELLMPVVKLWRKLWYGANVVSLPHWSNVRPETKHICEQFRNHHLENCKKDIAKDRRWRIRVYRRVFYGTEFVSWLIEVGLSKDRMEAVHYARHLVDGRVLRHINNVYHFEDKLLLYNFCSRI

Target ORF information:

RefSeq Version	NM_001275066.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster anchor, transcript variant B (anchor), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275066.1

ORF Insert Sequence:

ATGGACAGCT CCATGTACTA CGCCAGCCAG CGGATTGCTG CCAGCACAGA GGCTAATGTC 
AGAGTCATCA CCTCGACCAT GGCCTCATCT TTGGCCAATG GAACGGCGAC GCCACGCGCC 
GACGACGGGG GCGTATCCAT GAACAACTTT TACCCGGCGT TGGTTCAGTG TTTCGGCATC 
ATCATTTGCG GTTACATTGC CGGACGCTTC AAGATCATCA GCAATGCGGA AACCAAGGGT 
CTGGGCACGT TTGTGGGCAC CTTCGCCCTG CCCTCGCTGA TCTTCCTGTC CCTGGTCGAG 
CTCAACTGGA GCGCTGTCAA CTGGAGCTTC CTTCTGGCCA TGCTCGTCTC CAAGGCGGTG 
GTCTTCTTCG CCGTGCTGAT CATCTCGCTG CTGGTCGCCA GGCCCCTGAA CTACGCTCGC 
GGCGGCCTGA TGGCAATATT CTGCACCCAG AGCAATGACT TCGCTATCGG CTATCCCATA 
GTTATGGCTC TTTATAAGGA TGTCCATCCG GAATATGCCT CGTATCTGTA CCTGATGGCT 
CCTATCTCAC TGGCTATTCT AAATCCAGTT GGTCTGGTCC TCATGGAGAT CTCCAAGATA 
ATCAAGAACA AGGAGGATGT GACCCGCAAT CCACCATTGT GTCCAGAAAC TTGTCCCGCG 
GAACAGATGT CCAAACGGAA TCGCTGCCTG GGTGAACGGT CTATACTGGT TTTCAACACC 
CTAATTGCAC TGTTCTTTAA CCCGTTGCTG CTGATGACCT TGCTGGGTGT TGCAGGAGGA 
TTCCTGTTTC CCAACGGTCT GCCCGAAATG GTGTCCAGTA CGTTGCGTGT TCTTGGCCAG 
AGCTTTTCGG CAACGGCCTT GTTCCTACTC GGTCTTAAGA TCGTAGGTGG GACAGGATCC 
GAACGAAAGA GTATAGGCTT TCTATTGCCT GGTGTTCTTA TACTGGTTAA AATATTGGTG 
CTACCGCTCG TAATCCGACA GACGGTAAAC ATTATGCAGT CTGGCCAGAA CTTCAACGAC 
ACCACCGAGC TGAGCACTTT TGGCTTTCTC TACGGCACCT TTCCAGCTGC CCCCGGGGCT 
TTCGTCATTG CGACACAGTA CAACATGGAG GTTGAATTGG TTGCTCGAAG CATGGTTTTC 
TGTACATTCA TCTCGGCACC GCTGATGTTT ATATCAGCCA AAATGATATC GCTGACGAAC 
CTGAAGCCAT TGGACTATCT CCATGAGCTG GATGCCTTTT CGTTTGACAT CAGCGTGGCC 
GGCGCGGCTG CCTGTTGTAT CATGCTGGCA TTGTTGATCG TGACGAAGCG CTTCAAGCGT 
ATGCCCCAAA GAATAACTTT CTGTCTGGTG CTTTCTCAGC TCATGTGCTG CATAGGAGTA 
ATCCTCTGGT CCAAGATGGA GCACGTGCAC CGTTGGCCTT TGTACCTGCA GTTTCTTCTA 
TTTAATATCG GAACATACAG CACTCGTCTG TGGACTGGTC TTTTGGCCAT TTCATTGCTC 
TTCCTGCAGT GTCGCAGCTT GTGCTTTGTG CTGAAACTAT GGCCCTATAT GTTGGTGTTT 
GCCTGGGGTG TGCCGGCCGT ATTATCTGCC CTTTTGATTG CCTTCGACTC GAATGTGATG 
TCGGGAGAGA AACACAATCC GAGCTTTCAG TACGGAAATG CTCAGGCAGC CATCTCGGTG 
TTTATGCTCG TTATGTGCTT TATAGTAACC GTTGGCTGTT TGGTGCTGCA TCAGCGTTAT 
AAGAAACGAT ACGAAAAGTA CATGACCTAT TCACGTGAGT TGTCCAATCC GGAGTCGGAA 
TCATCCGATC TGCACTCGAC CATATCAACG ACTAATCTAT TGAGTCACAC GGATCAATCG 
TCCATCAGGC AAAGCGTTCG CACAGCTTCT TATTCGTCTT CATCCGACGA TGAGGAAATG 
ATACCAACAG CTGCGGGACT GCCAACCAGT AGGTCAAATG GTGCTGCCAG TGGAGGAGCA 
GGATCAGGCG GAGGAGGTTG TGGTTCTGGC AATGGAACTT GCTGTTCGGG CGGCGCGCCT 
GTGAGCAATT CCTCAACAAC CAGCACCGTG GTTGTGGACA TTGAGGATCT GTTGAATCGC 
ACACGCCAAC GAGCCAGTGG TGCCAACAAT AAAGATCTAA ACAAGATCGA AAGTGCACCT 
GCCACGGAAG AAATTCGCAA TCAAATGTGC AGCAGCTCCT TTAACTGTGG CCCAAGTACC 
TCACGTCAAA GTTGTCAGAC TATTATCGAG CGATATGAGG ATCAGAACAG AAGGGGACTT 
GAGCCCCTGG AACACCCGAG CGACGCTGAT GAGCACCAAA CTTTGAAGCA TACCGTGCTC 
TTGATCACTT TGCTGTGCTC CATGTTTGTC GGTCTGGCAG TGTCCATTTG GACATTGGTC 
ATGGAGGGAA TGTCGGGCAT ATATCTGGAA CTAAGTTTCC TCGATGCTTT CCTCAACTTT 
GGTCAAGGTC TGATTGTTTT GGCAGTGTTT ATCACCGACA TTGGCGAACT TCTAATGCCA 
GTTGTGAAAC TATGGCGCAA GTTATGGTAT GGAGCCAATG TGGTCAGCCT ACCGCACTGG 
TCAAACGTGC GGCCCGAAAC CAAGCATATT TGTGAACAAT TCCGTAATCA TCATTTGGAG 
AACTGCAAAA AGGATATTGC AAAGGACCGA AGGTGGCGTA TTCGTGTGTA TCGCAGAGTG 
TTCTACGGCA CCGAGTTCGT CAGCTGGCTC ATCGAGGTGG GACTGTCCAA GGATCGAATG 
GAGGCTGTGC ATTATGCCCG ACATCTGGTC GATGGCCGGG TTTTGCGACA CATAAACAAC 
GTTTATCACT TTGAGGATAA GCTGCTGCTC TACAATTTCT GCAGTCGCAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

anchor ( NM_140741.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648998.2 Drosophila melanogaster anchor, transcript variant A (anchor), mRNA.

Price & Availability↑

CloneID	OFa21024
Clone ID Related Accession (Same CDS sequence)	NM_140741.3
Accession Version	NM_140741.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	anchor, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140741.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAGCT CCATGTACTA CGCCAGCCAG CGGATTGCTG CCAGCACAGA GGCTAATGTC 
AGAGTCATCA CCTCGACCAT GGCCTCATCT TTGGCCAATG GAACGGCGAC GCCACGCGCC 
GACGACGGGG GCGTATCCAT GAACAACTTT TACCCGGCGT TGGTTCAGTG TTTCGGCATC 
ATCATTTGCG GTTACATTGC CGGACGCTTC AAGATCATCA GCAATGCGGA AACCAAGGGT 
CTGGGCACGT TTGTGGGCAC CTTCGCCCTG CCCTCGCTGA TCTTCCTGTC CCTGGTCGAG 
CTCAACTGGA GCGCTGTCAA CTGGAGCTTC CTTCTGGCCA TGCTCGTCTC CAAGGCGGTG 
GTCTTCTTCG CCGTGCTGAT CATCTCGCTG CTGGTCGCCA GGCCCCTGAA CTACGCTCGC 
GGCGGCCTGA TGGCAATATT CTGCACCCAG AGCAATGACT TCGCTATCGG CTATCCCATA 
GTTATGGCTC TTTATAAGGA TGTCCATCCG GAATATGCCT CGTATCTGTA CCTGATGGCT 
CCTATCTCAC TGGCTATTCT AAATCCAGTT GGTCTGGTCC TCATGGAGAT CTCCAAGATA 
ATCAAGAACA AGGAGGATGT GACCCGCAAT CCACCATTGT GTCCAGAAAC TTGTCCCGCG 
GAACAGATGT CCAAACGGAA TCGCTGCCTG GGTGAACGGT CTATACTGGT TTTCAACACC 
CTAATTGCAC TGTTCTTTAA CCCGTTGCTG CTGATGACCT TGCTGGGTGT TGCAGGAGGA 
TTCCTGTTTC CCAACGGTCT GCCCGAAATG GTGTCCAGTA CGTTGCGTGT TCTTGGCCAG 
AGCTTTTCGG CAACGGCCTT GTTCCTACTC GGTCTTAAGA TCGTAGGTGG GACAGGATCC 
GAACGAAAGA GTATAGGCTT TCTATTGCCT GGTGTTCTTA TACTGGTTAA AATATTGGTG 
CTACCGCTCG TAATCCGACA GACGGTAAAC ATTATGCAGT CTGGCCAGAA CTTCAACGAC 
ACCACCGAGC TGAGCACTTT TGGCTTTCTC TACGGCACCT TTCCAGCTGC CCCCGGGGCT 
TTCGTCATTG CGACACAGTA CAACATGGAG GTTGAATTGG TTGCTCGAAG CATGGTTTTC 
TGTACATTCA TCTCGGCACC GCTGATGTTT ATATCAGCCA AAATGATATC GCTGACGAAC 
CTGAAGCCAT TGGACTATCT CCATGAGCTG GATGCCTTTT CGTTTGACAT CAGCGTGGCC 
GGCGCGGCTG CCTGTTGTAT CATGCTGGCA TTGTTGATCG TGACGAAGCG CTTCAAGCGT 
ATGCCCCAAA GAATAACTTT CTGTCTGGTG CTTTCTCAGC TCATGTGCTG CATAGGAGTA 
ATCCTCTGGT CCAAGATGGA GCACGTGCAC CGTTGGCCTT TGTACCTGCA GTTTCTTCTA 
TTTAATATCG GAACATACAG CACTCGTCTG TGGACTGGTC TTTTGGCCAT TTCATTGCTC 
TTCCTGCAGT GTCGCAGCTT GTGCTTTGTG CTGAAACTAT GGCCCTATAT GTTGGTGTTT 
GCCTGGGGTG TGCCGGCCGT ATTATCTGCC CTTTTGATTG CCTTCGACTC GAATGTGATG 
TCGGGAGAGA AACACAATCC GAGCTTTCAG TACGGAAATG CTCAGGCAGC CATCTCGGTG 
TTTATGCTCG TTATGTGCTT TATAGTAACC GTTGGCTGTT TGGTGCTGCA TCAGCGTTAT 
AAGAAACGAT ACGAAAAGTA CATGACCTAT TCACAATCAT CCGATCTGCA CTCGACCATA 
TCAACGACTA ATCTATTGAG TCACACGGAT CAATCGTCCA TCAGGCAAAG CGTTCGCACA 
GCTTCTTATT CGTCTTCATC CGACGATGAG GAAATGATAC CAACAGCTGC GGGACTGCCA 
ACCAGTAGGT CAAATGGTGC TGCCAGTGGA GGAGCAGGAT CAGGCGGAGG AGGTTGTGGT 
TCTGGCAATG GAACTTGCTG TTCGGGCGGC GCGCCTGTGA GCAATTCCTC AACAACCAGC 
ACCGTGGTTG TGGACATTGA GGATCTGTTG AATCGCACAC GCCAACGAGC CAGTGGTGCC 
AACAATAAAG ATCTAAACAA GATCGAAAGT GCACCTGCCA CGGAAGAAAT TCGCAATCAA 
ATGTGCAGCA GCTCCTTTAA CTGTGGCCCA AGTACCTCAC GTCAAAGTTG TCAGACTATT 
ATCGAGCGAT ATGAGGATCA GAACAGAAGG GGACTTGAGC CCCTGGAACA CCCGAGCGAC 
GCTGATGAGC ACCAAACTTT GAAGCATACC GTGCTCTTGA TCACTTTGCT GTGCTCCATG 
TTTGTCGGTC TGGCAGTGTC CATTTGGACA TTGGTCATGG AGGGAATGTC GGGCATATAT 
CTGGAACTAA GTTTCCTCGA TGCTTTCCTC AACTTTGGTC AAGGTCTGAT TGTTTTGGCA 
GTGTTTATCA CCGACATTGG CGAACTTCTA ATGCCAGTTG TGAAACTATG GCGCAAGTTA 
TGGTATGGAG CCAATGTGGT CAGCCTACCG CACTGGTCAA ACGTGCGGCC CGAAACCAAG 
CATATTTGTG AACAATTCCG TAATCATCAT TTGGAGAACT GCAAAAAGGA TATTGCAAAG 
GACCGAAGGT GGCGTATTCG TGTGTATCGC AGAGTGTTCT ACGGCACCGA GTTCGTCAGC 
TGGCTCATCG AGGTGGGACT GTCCAAGGAT CGAATGGAGG CTGTGCATTA TGCCCGACAT 
CTGGTCGATG GCCGGGTTTT GCGACACATA AACAACGTTT ATCACTTTGA GGATAAGCTG 
CTGCTCTACA ATTTCTGCAG TCGCATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648998.2
CDS	480..3329
Translation	MDSSMYYASQRIAASTEANVRVITSTMASSLANGTATPRADDGGVSMNNFYPALVQCFGIIICGYIAGRFKIISNAETKGLGTFVGTFALPSLIFLSLVELNWSAVNWSFLLAMLVSKAVVFFAVLIISLLVARPLNYARGGLMAIFCTQSNDFAIGYPIVMALYKDVHPEYASYLYLMAPISLAILNPVGLVLMEISKIIKNKEDVTRNPPLCPETCPAEQMSKRNRCLGERSILVFNTLIALFFNPLLLMTLLGVAGGFLFPNGLPEMVSSTLRVLGQSFSATALFLLGLKIVGGTGSERKSIGFLLPGVLILVKILVLPLVIRQTVNIMQSGQNFNDTTELSTFGFLYGTFPAAPGAFVIATQYNMEVELVARSMVFCTFISAPLMFISAKMISLTNLKPLDYLHELDAFSFDISVAGAAACCIMLALLIVTKRFKRMPQRITFCLVLSQLMCCIGVILWSKMEHVHRWPLYLQFLLFNIGTYSTRLWTGLLAISLLFLQCRSLCFVLKLWPYMLVFAWGVPAVLSALLIAFDSNVMSGEKHNPSFQYGNAQAAISVFMLVMCFIVTVGCLVLHQRYKKRYEKYMTYSQSSDLHSTISTTNLLSHTDQSSIRQSVRTASYSSSSDDEEMIPTAAGLPTSRSNGAASGGAGSGGGGCGSGNGTCCSGGAPVSNSSTTSTVVVDIEDLLNRTRQRASGANNKDLNKIESAPATEEIRNQMCSSSFNCGPSTSRQSCQTIIERYEDQNRRGLEPLEHPSDADEHQTLKHTVLLITLLCSMFVGLAVSIWTLVMEGMSGIYLELSFLDAFLNFGQGLIVLAVFITDIGELLMPVVKLWRKLWYGANVVSLPHWSNVRPETKHICEQFRNHHLENCKKDIAKDRRWRIRVYRRVFYGTEFVSWLIEVGLSKDRMEAVHYARHLVDGRVLRHINNVYHFEDKLLLYNFCSRI

Target ORF information:

RefSeq Version	NM_140741.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster anchor, transcript variant A (anchor), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140741.3

ORF Insert Sequence:

ATGGACAGCT CCATGTACTA CGCCAGCCAG CGGATTGCTG CCAGCACAGA GGCTAATGTC 
AGAGTCATCA CCTCGACCAT GGCCTCATCT TTGGCCAATG GAACGGCGAC GCCACGCGCC 
GACGACGGGG GCGTATCCAT GAACAACTTT TACCCGGCGT TGGTTCAGTG TTTCGGCATC 
ATCATTTGCG GTTACATTGC CGGACGCTTC AAGATCATCA GCAATGCGGA AACCAAGGGT 
CTGGGCACGT TTGTGGGCAC CTTCGCCCTG CCCTCGCTGA TCTTCCTGTC CCTGGTCGAG 
CTCAACTGGA GCGCTGTCAA CTGGAGCTTC CTTCTGGCCA TGCTCGTCTC CAAGGCGGTG 
GTCTTCTTCG CCGTGCTGAT CATCTCGCTG CTGGTCGCCA GGCCCCTGAA CTACGCTCGC 
GGCGGCCTGA TGGCAATATT CTGCACCCAG AGCAATGACT TCGCTATCGG CTATCCCATA 
GTTATGGCTC TTTATAAGGA TGTCCATCCG GAATATGCCT CGTATCTGTA CCTGATGGCT 
CCTATCTCAC TGGCTATTCT AAATCCAGTT GGTCTGGTCC TCATGGAGAT CTCCAAGATA 
ATCAAGAACA AGGAGGATGT GACCCGCAAT CCACCATTGT GTCCAGAAAC TTGTCCCGCG 
GAACAGATGT CCAAACGGAA TCGCTGCCTG GGTGAACGGT CTATACTGGT TTTCAACACC 
CTAATTGCAC TGTTCTTTAA CCCGTTGCTG CTGATGACCT TGCTGGGTGT TGCAGGAGGA 
TTCCTGTTTC CCAACGGTCT GCCCGAAATG GTGTCCAGTA CGTTGCGTGT TCTTGGCCAG 
AGCTTTTCGG CAACGGCCTT GTTCCTACTC GGTCTTAAGA TCGTAGGTGG GACAGGATCC 
GAACGAAAGA GTATAGGCTT TCTATTGCCT GGTGTTCTTA TACTGGTTAA AATATTGGTG 
CTACCGCTCG TAATCCGACA GACGGTAAAC ATTATGCAGT CTGGCCAGAA CTTCAACGAC 
ACCACCGAGC TGAGCACTTT TGGCTTTCTC TACGGCACCT TTCCAGCTGC CCCCGGGGCT 
TTCGTCATTG CGACACAGTA CAACATGGAG GTTGAATTGG TTGCTCGAAG CATGGTTTTC 
TGTACATTCA TCTCGGCACC GCTGATGTTT ATATCAGCCA AAATGATATC GCTGACGAAC 
CTGAAGCCAT TGGACTATCT CCATGAGCTG GATGCCTTTT CGTTTGACAT CAGCGTGGCC 
GGCGCGGCTG CCTGTTGTAT CATGCTGGCA TTGTTGATCG TGACGAAGCG CTTCAAGCGT 
ATGCCCCAAA GAATAACTTT CTGTCTGGTG CTTTCTCAGC TCATGTGCTG CATAGGAGTA 
ATCCTCTGGT CCAAGATGGA GCACGTGCAC CGTTGGCCTT TGTACCTGCA GTTTCTTCTA 
TTTAATATCG GAACATACAG CACTCGTCTG TGGACTGGTC TTTTGGCCAT TTCATTGCTC 
TTCCTGCAGT GTCGCAGCTT GTGCTTTGTG CTGAAACTAT GGCCCTATAT GTTGGTGTTT 
GCCTGGGGTG TGCCGGCCGT ATTATCTGCC CTTTTGATTG CCTTCGACTC GAATGTGATG 
TCGGGAGAGA AACACAATCC GAGCTTTCAG TACGGAAATG CTCAGGCAGC CATCTCGGTG 
TTTATGCTCG TTATGTGCTT TATAGTAACC GTTGGCTGTT TGGTGCTGCA TCAGCGTTAT 
AAGAAACGAT ACGAAAAGTA CATGACCTAT TCACAATCAT CCGATCTGCA CTCGACCATA 
TCAACGACTA ATCTATTGAG TCACACGGAT CAATCGTCCA TCAGGCAAAG CGTTCGCACA 
GCTTCTTATT CGTCTTCATC CGACGATGAG GAAATGATAC CAACAGCTGC GGGACTGCCA 
ACCAGTAGGT CAAATGGTGC TGCCAGTGGA GGAGCAGGAT CAGGCGGAGG AGGTTGTGGT 
TCTGGCAATG GAACTTGCTG TTCGGGCGGC GCGCCTGTGA GCAATTCCTC AACAACCAGC 
ACCGTGGTTG TGGACATTGA GGATCTGTTG AATCGCACAC GCCAACGAGC CAGTGGTGCC 
AACAATAAAG ATCTAAACAA GATCGAAAGT GCACCTGCCA CGGAAGAAAT TCGCAATCAA 
ATGTGCAGCA GCTCCTTTAA CTGTGGCCCA AGTACCTCAC GTCAAAGTTG TCAGACTATT 
ATCGAGCGAT ATGAGGATCA GAACAGAAGG GGACTTGAGC CCCTGGAACA CCCGAGCGAC 
GCTGATGAGC ACCAAACTTT GAAGCATACC GTGCTCTTGA TCACTTTGCT GTGCTCCATG 
TTTGTCGGTC TGGCAGTGTC CATTTGGACA TTGGTCATGG AGGGAATGTC GGGCATATAT 
CTGGAACTAA GTTTCCTCGA TGCTTTCCTC AACTTTGGTC AAGGTCTGAT TGTTTTGGCA 
GTGTTTATCA CCGACATTGG CGAACTTCTA ATGCCAGTTG TGAAACTATG GCGCAAGTTA 
TGGTATGGAG CCAATGTGGT CAGCCTACCG CACTGGTCAA ACGTGCGGCC CGAAACCAAG 
CATATTTGTG AACAATTCCG TAATCATCAT TTGGAGAACT GCAAAAAGGA TATTGCAAAG 
GACCGAAGGT GGCGTATTCG TGTGTATCGC AGAGTGTTCT ACGGCACCGA GTTCGTCAGC 
TGGCTCATCG AGGTGGGACT GTCCAAGGAT CGAATGGAGG CTGTGCATTA TGCCCGACAT 
CTGGTCGATG GCCGGGTTTT GCGACACATA AACAACGTTT ATCACTTTGA GGATAAGCTG 
CTGCTCTACA ATTTCTGCAG TCGCATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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anchor cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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