CG6512 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG6512
Entrez Gene ID	39922
Full Name	CG6512 gene product from transcript CG6512-RB
Synonyms	AFG3L2,DmCG6512,Dmel\CG6512
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 74A1-74A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_168722.3	NP_730250.1	uncharacterized protein, isoform B
NM_168720.3	NP_730248.2	uncharacterized protein, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	AFG3L2	NP_006787.2
Pan troglodytes (chimpanzee)	AFG3L2	XP_512199.2
Saccharomyces cerevisiae (baker's yeast)	AFG3	NP_010933.1
Eremothecium gossypii	AGOS_AAR025C	NP_982566.2
Arabidopsis thaliana (thale cress)	ftsh10	NP_172231.2
Bos taurus (cattle)	AFG3L2	NP_001039676.1
Mus musculus (house mouse)	Afg3l2	NP_081406.1
Arabidopsis thaliana (thale cress)	ftsh3	NP_850129.1
	AFG3L2	XP_001094146.1
Gallus gallus (chicken)	AFG3L2	XP_419121.2
Drosophila melanogaster (fruit fly)	CG6512	NP_730248.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006949	XP_308807.4
Caenorhabditis elegans (roundworm)	spg-7	NP_491165.2
Danio rerio (zebrafish)	afg3l2	NP_001104667.1
Oryza sativa (rice)	Os05g0458400	NP_001055745.1
Canis lupus familiaris (dog)	AFG3L2	XP_547682.2
Rattus norvegicus (Norway rat)	Afg3l2	NP_001128336.1
Oryza sativa (rice)	Os01g0842600	NP_001044774.1
Xenopus tropicalis (tropical clawed frog)	afg3l2	NP_001072759.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG6512 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG6512 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18797	NM_168722.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG6512), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa18798	NM_168720.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG6512), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG6512 ( NM_168722.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730250.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG6512), mRNA.

Price & Availability↑

CloneID	OFa18797
Clone ID Related Accession (Same CDS sequence)	NM_168722.3
Accession Version	NM_168722.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168722.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCAGCA ACAGCTCGAG GGGGCCAGCA GGAAAAGGAG GCAACTCGCT GGGCGGACGA 
CCTCTGGGCG AGAACGGTGG CGGCGGGGAC AGGGAGCGGT GGATCCTTCT GGGTGCCATC 
GGAGCCGTCG TCCTGGTGGG TTCGTTCGCC TTCTTCGAGA TGGGCTACAA GGAGATCTCG 
TGGAAGGAGT TCGTCAACAG CTACCTGTCG AAGGGCGTCG TGGAGAAGCT GGAGGTGGTT 
AACAAGAAGT GGGTGAGAGT GCGTCTGCAG CAGAACAGCA ACAGCGGCAG CGGCGTCTTG 
TGGTTCAACA TCGGCAGTGT CGATAGCTTC GAGCGAAACC TGGAGGCAGC CCAGACGGAG 
CAGGGAACGG AATCCATCAA CTTTGTGCCC GTAATCTACC GCAACGAGGT GGAGGCCGCC 
AGTCTGACGG GTCTGCTGCC CACGTTGCTC ATCATAGGCT TTCTGGTCTA TATGATGCGG 
AAGTCGGCCG ATATGATGGG TGGAGGCCGT GGGCGCAAGG GTGGTGGTCT TTTTGGTGGC 
GTCATGCAGT CCACCGCAAA GCTAACCAAT CCCAATGAGA TTGGTGTGGG TTTCAAGGAT 
GTTGCCGGTT GCGAGGAGGC CAAAATCGAA ATCATGGAGT TCGTTAACTT CCTGAAGAAC 
CCGCAGCAGT ATATTGATTT AGGCGCCAAG ATACCGAAGG GTGCCATGCT AACTGGCCCA 
CCCGGAACGG GTAAAACGCT ACTGGCCAAG GCTACCGCCG GCGAGGCGAA TGTACCGTTT 
ATTACCGTGT CGGGATCCGA GTTCCTCGAG ATGTTTGTGG GCGTGGGACC TTCTCGTGTC 
CGCGACATGT TCGCCATGGC CCGAAAGCAT GCTCCCTGCA TCCTCTTCAT CGACGAAATC 
GATGCTGTGG GTCGCAAACG TGGTGGCAAG ACCTTCGGTG GCCACTCGGA GCAAGAGAAT 
ACCCTTAACC AGCTTCTGGT GGAAATGGAT GGCTTCAATA CCACTACGAA TGTGGTCGTA 
CTGGCCGCCA CTAATCGTGT TGATATTCTG GACAAGGCTC TCATGCGACC AGGTCGTTTC 
GATCGACAGA TTTATGTACC AGCACCTGAT ATTAAGGGCA GGGCGAGCAT CTTTAAGGTG 
CATCTGGGTA ACCTGAAAAC GTCGCTGGAC AAGAACGAGC TGAGCAGAAA AATGGCTGCA 
CTGACACCAG GCTTCACAGG CGCTGACATC GCGAATGTTT GCAACGAAGC TGCTTTGATT 
GCCGCCCGCG ACTCCAAGGA TTCGATCGTA CTCAAGCACT TCGAGCAGGC CATTGAGCGT 
GTTATCGCTG GCATGGAGAA GAAGACCAAT GTTCTGGCGC CGGAGGAAAA GCGAACGGTG 
GCACACCACG AGGCTGGCCA TGCGGTGGCC GGTTGGTTCC TGGAGCACGC AGATCCCCTG 
CTGAAGGTCT CGATTATACC ACGCGGCAAG GGTCTGGGCT ACGCCCAGTA TCTGCCCAAG 
GATCACTACT TGCTGTCCAA GGAGCAGTTG TTCGATCGCA TGTGCATGAC GCTCGGCGGA 
CGTGTGGCCG AAGAGCTGTT CTTTAATCGC ATTACCACTG GTGCTCAGGA TGATCTAAAG 
AAGATCACCG ATATTGCCTA CTCCCAGGTG GTGCGTTTCG GCATGAACGA GAAGGTCGGG 
CAGGTCAGCT TCGATGTGGG CCAGGCCGGT GATCCCGTTT TCAGCAAACC ATACTCGGAG 
GACACAGCGC AGCTGATCGA CAACGAGGTG AGGTCGATTA TCAAATGTGC CCACGAGGCC 
ACCACTAGTC TGCTGACCAA GCACAAGGAG AATGTTCAAA AAGTGGCGGA AAGACTGCTC 
CAAAACGAAG TGCTCAGCCG TGATGATATG ATTGAGCTGC TGGGCCCACG ACCCTTCAAA 
GAGAAGTCAA CCTACGAGGA GTTCGTCGAG GGTACCGGCT CCTTCGAGGA GGACACCACT 
CTGCCCGAAG GCCTCAAGAG CTGGAACAAG GAAAAGGAAC GAACGGAGCC TCTGGACGCT 
GGCAGCACGC CATCATCGCC GCCCACCAAG CCCGTAACTG CCCAGAGCAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730250.1
CDS	225..2318
Translation	MFSNSSRGPAGKGGNSLGGRPLGENGGGGDRERWILLGAIGAVVLVGSFAFFEMGYKEISWKEFVNSYLSKGVVEKLEVVNKKWVRVRLQQNSNSGSGVLWFNIGSVDSFERNLEAAQTEQGTESINFVPVIYRNEVEAASLTGLLPTLLIIGFLVYMMRKSADMMGGGRGRKGGGLFGGVMQSTAKLTNPNEIGVGFKDVAGCEEAKIEIMEFVNFLKNPQQYIDLGAKIPKGAMLTGPPGTGKTLLAKATAGEANVPFITVSGSEFLEMFVGVGPSRVRDMFAMARKHAPCILFIDEIDAVGRKRGGKTFGGHSEQENTLNQLLVEMDGFNTTTNVVVLAATNRVDILDKALMRPGRFDRQIYVPAPDIKGRASIFKVHLGNLKTSLDKNELSRKMAALTPGFTGADIANVCNEAALIAARDSKDSIVLKHFEQAIERVIAGMEKKTNVLAPEEKRTVAHHEAGHAVAGWFLEHADPLLKVSIIPRGKGLGYAQYLPKDHYLLSKEQLFDRMCMTLGGRVAEELFFNRITTGAQDDLKKITDIAYSQVVRFGMNEKVGQVSFDVGQAGDPVFSKPYSEDTAQLIDNEVRSIIKCAHEATTSLLTKHKENVQKVAERLLQNEVLSRDDMIELLGPRPFKEKSTYEEFVEGTGSFEEDTTLPEGLKSWNKEKERTEPLDAGSTPSSPPTKPVTAQSS

Target ORF information:

RefSeq Version	NM_168722.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG6512), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168722.3

ORF Insert Sequence:

ATGTTCAGCA ACAGCTCGAG GGGGCCAGCA GGAAAAGGAG GCAACTCGCT GGGCGGACGA 
CCTCTGGGCG AGAACGGTGG CGGCGGGGAC AGGGAGCGGT GGATCCTTCT GGGTGCCATC 
GGAGCCGTCG TCCTGGTGGG TTCGTTCGCC TTCTTCGAGA TGGGCTACAA GGAGATCTCG 
TGGAAGGAGT TCGTCAACAG CTACCTGTCG AAGGGCGTCG TGGAGAAGCT GGAGGTGGTT 
AACAAGAAGT GGGTGAGAGT GCGTCTGCAG CAGAACAGCA ACAGCGGCAG CGGCGTCTTG 
TGGTTCAACA TCGGCAGTGT CGATAGCTTC GAGCGAAACC TGGAGGCAGC CCAGACGGAG 
CAGGGAACGG AATCCATCAA CTTTGTGCCC GTAATCTACC GCAACGAGGT GGAGGCCGCC 
AGTCTGACGG GTCTGCTGCC CACGTTGCTC ATCATAGGCT TTCTGGTCTA TATGATGCGG 
AAGTCGGCCG ATATGATGGG TGGAGGCCGT GGGCGCAAGG GTGGTGGTCT TTTTGGTGGC 
GTCATGCAGT CCACCGCAAA GCTAACCAAT CCCAATGAGA TTGGTGTGGG TTTCAAGGAT 
GTTGCCGGTT GCGAGGAGGC CAAAATCGAA ATCATGGAGT TCGTTAACTT CCTGAAGAAC 
CCGCAGCAGT ATATTGATTT AGGCGCCAAG ATACCGAAGG GTGCCATGCT AACTGGCCCA 
CCCGGAACGG GTAAAACGCT ACTGGCCAAG GCTACCGCCG GCGAGGCGAA TGTACCGTTT 
ATTACCGTGT CGGGATCCGA GTTCCTCGAG ATGTTTGTGG GCGTGGGACC TTCTCGTGTC 
CGCGACATGT TCGCCATGGC CCGAAAGCAT GCTCCCTGCA TCCTCTTCAT CGACGAAATC 
GATGCTGTGG GTCGCAAACG TGGTGGCAAG ACCTTCGGTG GCCACTCGGA GCAAGAGAAT 
ACCCTTAACC AGCTTCTGGT GGAAATGGAT GGCTTCAATA CCACTACGAA TGTGGTCGTA 
CTGGCCGCCA CTAATCGTGT TGATATTCTG GACAAGGCTC TCATGCGACC AGGTCGTTTC 
GATCGACAGA TTTATGTACC AGCACCTGAT ATTAAGGGCA GGGCGAGCAT CTTTAAGGTG 
CATCTGGGTA ACCTGAAAAC GTCGCTGGAC AAGAACGAGC TGAGCAGAAA AATGGCTGCA 
CTGACACCAG GCTTCACAGG CGCTGACATC GCGAATGTTT GCAACGAAGC TGCTTTGATT 
GCCGCCCGCG ACTCCAAGGA TTCGATCGTA CTCAAGCACT TCGAGCAGGC CATTGAGCGT 
GTTATCGCTG GCATGGAGAA GAAGACCAAT GTTCTGGCGC CGGAGGAAAA GCGAACGGTG 
GCACACCACG AGGCTGGCCA TGCGGTGGCC GGTTGGTTCC TGGAGCACGC AGATCCCCTG 
CTGAAGGTCT CGATTATACC ACGCGGCAAG GGTCTGGGCT ACGCCCAGTA TCTGCCCAAG 
GATCACTACT TGCTGTCCAA GGAGCAGTTG TTCGATCGCA TGTGCATGAC GCTCGGCGGA 
CGTGTGGCCG AAGAGCTGTT CTTTAATCGC ATTACCACTG GTGCTCAGGA TGATCTAAAG 
AAGATCACCG ATATTGCCTA CTCCCAGGTG GTGCGTTTCG GCATGAACGA GAAGGTCGGG 
CAGGTCAGCT TCGATGTGGG CCAGGCCGGT GATCCCGTTT TCAGCAAACC ATACTCGGAG 
GACACAGCGC AGCTGATCGA CAACGAGGTG AGGTCGATTA TCAAATGTGC CCACGAGGCC 
ACCACTAGTC TGCTGACCAA GCACAAGGAG AATGTTCAAA AAGTGGCGGA AAGACTGCTC 
CAAAACGAAG TGCTCAGCCG TGATGATATG ATTGAGCTGC TGGGCCCACG ACCCTTCAAA 
GAGAAGTCAA CCTACGAGGA GTTCGTCGAG GGTACCGGCT CCTTCGAGGA GGACACCACT 
CTGCCCGAAG GCCTCAAGAG CTGGAACAAG GAAAAGGAAC GAACGGAGCC TCTGGACGCT 
GGCAGCACGC CATCATCGCC GCCCACCAAG CCCGTAACTG CCCAGAGCAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG6512 ( NM_168720.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730248.2 Drosophila melanogaster uncharacterized protein, transcript variant A (CG6512), mRNA.

Price & Availability↑

CloneID	OFa18798
Clone ID Related Accession (Same CDS sequence)	NM_168720.3
Accession Version	NM_168720.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168720.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGTTCC GGCTGCTTGG CACGGCCAAA ACGATGGCCG GAATGATGCG GCGCAACTAC 
ACGGCGGGTT CCTTGCCGGG AAAGGGACAA CGCACACACA TGGGTGCCAC GGGCGCCGGG 
GTTTACATCC TGCCCACGGA GCTGTCGCCC CTGCTGCGCT ACGTGGTCAA ACAGATCCAG 
CTGCTGTGCA AAAAGCCGCC GAAGGGATTT GAAAAGTATT TCGAAGCCGG CGGCAAGTCG 
TCTGGCCAAC CGAAGGGATC CGTCGGCGAT AAGAAGCCAC CCGCGGGTTC CAGCAAATCC 
GCCTCGGAGA AGCCCAGCAC ATCGACACCC GCCTCAGTTA AACCCAGCAG ATCCCAAACA 
GACGCGAAAT CGGACTGGAA CTTCGGCATG TTCAGCAACA GCTCGAGGGG GCCAGCAGGA 
AAAGGAGGCA ACTCGCTGGG CGGACGACCT CTGGGCGAGA ACGGTGGCGG CGGGGACAGG 
GAGCGGTGGA TCCTTCTGGG TGCCATCGGA GCCGTCGTCC TGGTGGGTTC GTTCGCCTTC 
TTCGAGATGG GCTACAAGGA GATCTCGTGG AAGGAGTTCG TCAACAGCTA CCTGTCGAAG 
GGCGTCGTGG AGAAGCTGGA GGTGGTTAAC AAGAAGTGGG TGAGAGTGCG TCTGCAGCAG 
AACAGCAACA GCGGCAGCGG CGTCTTGTGG TTCAACATCG GCAGTGTCGA TAGCTTCGAG 
CGAAACCTGG AGGCAGCCCA GACGGAGCAG GGAACGGAAT CCATCAACTT TGTGCCCGTA 
ATCTACCGCA ACGAGGTGGA GGCCGCCAGT CTGACGGGTC TGCTGCCCAC GTTGCTCATC 
ATAGGCTTTC TGGTCTATAT GATGCGGAAG TCGGCCGATA TGATGGGTGG AGGCCGTGGG 
CGCAAGGGTG GTGGTCTTTT TGGTGGCGTC ATGCAGTCCA CCGCAAAGCT AACCAATCCC 
AATGAGATTG GTGTGGGTTT CAAGGATGTT GCCGGTTGCG AGGAGGCCAA AATCGAAATC 
ATGGAGTTCG TTAACTTCCT GAAGAACCCG CAGCAGTATA TTGATTTAGG CGCCAAGATA 
CCGAAGGGTG CCATGCTAAC TGGCCCACCC GGAACGGGTA AAACGCTACT GGCCAAGGCT 
ACCGCCGGCG AGGCGAATGT ACCGTTTATT ACCGTGTCGG GATCCGAGTT CCTCGAGATG 
TTTGTGGGCG TGGGACCTTC TCGTGTCCGC GACATGTTCG CCATGGCCCG AAAGCATGCT 
CCCTGCATCC TCTTCATCGA CGAAATCGAT GCTGTGGGTC GCAAACGTGG TGGCAAGACC 
TTCGGTGGCC ACTCGGAGCA AGAGAATACC CTTAACCAGC TTCTGGTGGA AATGGATGGC 
TTCAATACCA CTACGAATGT GGTCGTACTG GCCGCCACTA ATCGTGTTGA TATTCTGGAC 
AAGGCTCTCA TGCGACCAGG TCGTTTCGAT CGACAGATTT ATGTACCAGC ACCTGATATT 
AAGGGCAGGG CGAGCATCTT TAAGGTGCAT CTGGGTAACC TGAAAACGTC GCTGGACAAG 
AACGAGCTGA GCAGAAAAAT GGCTGCACTG ACACCAGGCT TCACAGGCGC TGACATCGCG 
AATGTTTGCA ACGAAGCTGC TTTGATTGCC GCCCGCGACT CCAAGGATTC GATCGTACTC 
AAGCACTTCG AGCAGGCCAT TGAGCGTGTT ATCGCTGGCA TGGAGAAGAA GACCAATGTT 
CTGGCGCCGG AGGAAAAGCG AACGGTGGCA CACCACGAGG CTGGCCATGC GGTGGCCGGT 
TGGTTCCTGG AGCACGCAGA TCCCCTGCTG AAGGTCTCGA TTATACCACG CGGCAAGGGT 
CTGGGCTACG CCCAGTATCT GCCCAAGGAT CACTACTTGC TGTCCAAGGA GCAGTTGTTC 
GATCGCATGT GCATGACGCT CGGCGGACGT GTGGCCGAAG AGCTGTTCTT TAATCGCATT 
ACCACTGGTG CTCAGGATGA TCTAAAGAAG ATCACCGATA TTGCCTACTC CCAGGTGGTG 
CGTTTCGGCA TGAACGAGAA GGTCGGGCAG GTCAGCTTCG ATGTGGGCCA GGCCGGTGAT 
CCCGTTTTCA GCAAACCATA CTCGGAGGAC ACAGCGCAGC TGATCGACAA CGAGGTGAGG 
TCGATTATCA AATGTGCCCA CGAGGCCACC ACTAGTCTGC TGACCAAGCA CAAGGAGAAT 
GTTCAAAAAG TGGCGGAAAG ACTGCTCCAA AACGAAGTGC TCAGCCGTGA TGATATGATT 
GAGCTGCTGG GCCCACGACC CTTCAAAGAG AAGTCAACCT ACGAGGAGTT CGTCGAGGGT 
ACCGGCTCCT TCGAGGAGGA CACCACTCTG CCCGAAGGCC TCAAGAGCTG GAACAAGGAA 
AAGGAACGAA CGGAGCCTCT GGACGCTGGC AGCACGCCAT CATCGCCGCC CACCAAGCCC 
GTAACTGCCC AGAGCAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730248.2
CDS	261..2741
Translation	MAFRLLGTAKTMAGMMRRNYTAGSLPGKGQRTHMGATGAGVYILPTELSPLLRYVVKQIQLLCKKPPKGFEKYFEAGGKSSGQPKGSVGDKKPPAGSSKSASEKPSTSTPASVKPSRSQTDAKSDWNFGMFSNSSRGPAGKGGNSLGGRPLGENGGGGDRERWILLGAIGAVVLVGSFAFFEMGYKEISWKEFVNSYLSKGVVEKLEVVNKKWVRVRLQQNSNSGSGVLWFNIGSVDSFERNLEAAQTEQGTESINFVPVIYRNEVEAASLTGLLPTLLIIGFLVYMMRKSADMMGGGRGRKGGGLFGGVMQSTAKLTNPNEIGVGFKDVAGCEEAKIEIMEFVNFLKNPQQYIDLGAKIPKGAMLTGPPGTGKTLLAKATAGEANVPFITVSGSEFLEMFVGVGPSRVRDMFAMARKHAPCILFIDEIDAVGRKRGGKTFGGHSEQENTLNQLLVEMDGFNTTTNVVVLAATNRVDILDKALMRPGRFDRQIYVPAPDIKGRASIFKVHLGNLKTSLDKNELSRKMAALTPGFTGADIANVCNEAALIAARDSKDSIVLKHFEQAIERVIAGMEKKTNVLAPEEKRTVAHHEAGHAVAGWFLEHADPLLKVSIIPRGKGLGYAQYLPKDHYLLSKEQLFDRMCMTLGGRVAEELFFNRITTGAQDDLKKITDIAYSQVVRFGMNEKVGQVSFDVGQAGDPVFSKPYSEDTAQLIDNEVRSIIKCAHEATTSLLTKHKENVQKVAERLLQNEVLSRDDMIELLGPRPFKEKSTYEEFVEGTGSFEEDTTLPEGLKSWNKEKERTEPLDAGSTPSSPPTKPVTAQSS

Target ORF information:

RefSeq Version	NM_168720.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG6512), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168720.3

ORF Insert Sequence:

ATGGCGTTCC GGCTGCTTGG CACGGCCAAA ACGATGGCCG GAATGATGCG GCGCAACTAC 
ACGGCGGGTT CCTTGCCGGG AAAGGGACAA CGCACACACA TGGGTGCCAC GGGCGCCGGG 
GTTTACATCC TGCCCACGGA GCTGTCGCCC CTGCTGCGCT ACGTGGTCAA ACAGATCCAG 
CTGCTGTGCA AAAAGCCGCC GAAGGGATTT GAAAAGTATT TCGAAGCCGG CGGCAAGTCG 
TCTGGCCAAC CGAAGGGATC CGTCGGCGAT AAGAAGCCAC CCGCGGGTTC CAGCAAATCC 
GCCTCGGAGA AGCCCAGCAC ATCGACACCC GCCTCAGTTA AACCCAGCAG ATCCCAAACA 
GACGCGAAAT CGGACTGGAA CTTCGGCATG TTCAGCAACA GCTCGAGGGG GCCAGCAGGA 
AAAGGAGGCA ACTCGCTGGG CGGACGACCT CTGGGCGAGA ACGGTGGCGG CGGGGACAGG 
GAGCGGTGGA TCCTTCTGGG TGCCATCGGA GCCGTCGTCC TGGTGGGTTC GTTCGCCTTC 
TTCGAGATGG GCTACAAGGA GATCTCGTGG AAGGAGTTCG TCAACAGCTA CCTGTCGAAG 
GGCGTCGTGG AGAAGCTGGA GGTGGTTAAC AAGAAGTGGG TGAGAGTGCG TCTGCAGCAG 
AACAGCAACA GCGGCAGCGG CGTCTTGTGG TTCAACATCG GCAGTGTCGA TAGCTTCGAG 
CGAAACCTGG AGGCAGCCCA GACGGAGCAG GGAACGGAAT CCATCAACTT TGTGCCCGTA 
ATCTACCGCA ACGAGGTGGA GGCCGCCAGT CTGACGGGTC TGCTGCCCAC GTTGCTCATC 
ATAGGCTTTC TGGTCTATAT GATGCGGAAG TCGGCCGATA TGATGGGTGG AGGCCGTGGG 
CGCAAGGGTG GTGGTCTTTT TGGTGGCGTC ATGCAGTCCA CCGCAAAGCT AACCAATCCC 
AATGAGATTG GTGTGGGTTT CAAGGATGTT GCCGGTTGCG AGGAGGCCAA AATCGAAATC 
ATGGAGTTCG TTAACTTCCT GAAGAACCCG CAGCAGTATA TTGATTTAGG CGCCAAGATA 
CCGAAGGGTG CCATGCTAAC TGGCCCACCC GGAACGGGTA AAACGCTACT GGCCAAGGCT 
ACCGCCGGCG AGGCGAATGT ACCGTTTATT ACCGTGTCGG GATCCGAGTT CCTCGAGATG 
TTTGTGGGCG TGGGACCTTC TCGTGTCCGC GACATGTTCG CCATGGCCCG AAAGCATGCT 
CCCTGCATCC TCTTCATCGA CGAAATCGAT GCTGTGGGTC GCAAACGTGG TGGCAAGACC 
TTCGGTGGCC ACTCGGAGCA AGAGAATACC CTTAACCAGC TTCTGGTGGA AATGGATGGC 
TTCAATACCA CTACGAATGT GGTCGTACTG GCCGCCACTA ATCGTGTTGA TATTCTGGAC 
AAGGCTCTCA TGCGACCAGG TCGTTTCGAT CGACAGATTT ATGTACCAGC ACCTGATATT 
AAGGGCAGGG CGAGCATCTT TAAGGTGCAT CTGGGTAACC TGAAAACGTC GCTGGACAAG 
AACGAGCTGA GCAGAAAAAT GGCTGCACTG ACACCAGGCT TCACAGGCGC TGACATCGCG 
AATGTTTGCA ACGAAGCTGC TTTGATTGCC GCCCGCGACT CCAAGGATTC GATCGTACTC 
AAGCACTTCG AGCAGGCCAT TGAGCGTGTT ATCGCTGGCA TGGAGAAGAA GACCAATGTT 
CTGGCGCCGG AGGAAAAGCG AACGGTGGCA CACCACGAGG CTGGCCATGC GGTGGCCGGT 
TGGTTCCTGG AGCACGCAGA TCCCCTGCTG AAGGTCTCGA TTATACCACG CGGCAAGGGT 
CTGGGCTACG CCCAGTATCT GCCCAAGGAT CACTACTTGC TGTCCAAGGA GCAGTTGTTC 
GATCGCATGT GCATGACGCT CGGCGGACGT GTGGCCGAAG AGCTGTTCTT TAATCGCATT 
ACCACTGGTG CTCAGGATGA TCTAAAGAAG ATCACCGATA TTGCCTACTC CCAGGTGGTG 
CGTTTCGGCA TGAACGAGAA GGTCGGGCAG GTCAGCTTCG ATGTGGGCCA GGCCGGTGAT 
CCCGTTTTCA GCAAACCATA CTCGGAGGAC ACAGCGCAGC TGATCGACAA CGAGGTGAGG 
TCGATTATCA AATGTGCCCA CGAGGCCACC ACTAGTCTGC TGACCAAGCA CAAGGAGAAT 
GTTCAAAAAG TGGCGGAAAG ACTGCTCCAA AACGAAGTGC TCAGCCGTGA TGATATGATT 
GAGCTGCTGG GCCCACGACC CTTCAAAGAG AAGTCAACCT ACGAGGAGTT CGTCGAGGGT 
ACCGGCTCCT TCGAGGAGGA CACCACTCTG CCCGAAGGCC TCAAGAGCTG GAACAAGGAA 
AAGGAACGAA CGGAGCCTCT GGACGCTGGC AGCACGCCAT CATCGCCGCC CACCAAGCCC 
GTAACTGCCC AGAGCAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG6512 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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