Fit2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fit2
Entrez Gene ID	39907
Full Name	Fermitin 2
Synonyms	CG7729,Dmel\CG7729
General protein information	Preferred Names Fermitin 2 Names CG7729 gene product from transcript CG7729-RA CG7729-PA Fit2-PA fermitin 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 73E4-73E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_140690.3	NP_648947.1	fermitin 2

Pathways from BioSystems

Homologies

	FERMT2	XP_001082931.1
Mus musculus (house mouse)	Fermt2	NP_666166.2
Bos taurus (cattle)	FERMT2	NP_001094734.1
Drosophila melanogaster (fruit fly)	Fit2	NP_648947.1
Drosophila melanogaster (fruit fly)	Fit1	NP_647858.2
Pan troglodytes (chimpanzee)	FERMT2	XP_003314374.1
Gallus gallus (chicken)	PLEKHC1	XP_004936582.1
Danio rerio (zebrafish)	fermt2	NP_001243144.1
Canis lupus familiaris (dog)	FERMT2	XP_547815.5
Rattus norvegicus (Norway rat)	Fermt2	NP_001011915.1
Caenorhabditis elegans (roundworm)	unc-112	NP_506628.1
Homo sapiens (human)	FERMT2	NP_001128471.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002054	XP_320993.2
Xenopus tropicalis (tropical clawed frog)	fermt2	XP_004917211.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Fit2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fit2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18783	NM_140690.3 Latest version!	Drosophila melanogaster fermitin 2 (Fit2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fit2 ( NM_140690.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648947.1 Drosophila melanogaster fermitin 2 (Fit2), mRNA.

Price & Availability↑

CloneID	OFa18783
Clone ID Related Accession (Same CDS sequence)	NM_140690.3
Accession Version	NM_140690.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fermitin 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140690.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCCACG TCGGCGAGAA CTCGTGGAAT CTGCGGATCT TCATCACGGA TCTGGCGCTG 
GAGAAGACGA TGCGCGTGCG CGGTGACCAG CACATTGGGG GCATTATGCT GCAACTGGTG 
GATCCGGAGA ATCCCAAGGA CTGGTCGGAT CATGCTCTGT GGTGGCCAGC GCGGAATGTT 
TGGCTCAGCC GAACCAAACT TACCCTGGAT CAGTGCGGTG TCCAGGCGGA TAGCCTTCTG 
CACTTCACGC CCATGCACAA GATACTGCGG GTGCAGCTGC CGGATTTGAG GTACCTGGAC 
TCCCGGGTGG ATTACTCCGT AAAGACGTTC TCGGCGGTGG TTAATCTATG CAAGCAGTTG 
GATATCCGCT ATCCGGAGGA GTTGTCCCTG TGCAAACCAC TGGAAGCGGA GCACCTGAAG 
CGTAACTTCG CCCAGGTGCC GCATCAGAAG CGAGTGCCAA TCGCTGAACC AGATGGCACC 
ACTTACCTGC AGCCTGCTGC GGACACCAAC TCATTTGTGC CCATCAGCAC GTCGTTTCAT 
GGAGAAGGCG GAAGCACGGG CAGTCTGGAC AAGCCCTCTG CACCGGGATC CTTCTTCTGT 
GCTCCTCTGT CGCCGCACAA TCACCGAGCG CGCTCGCCAG TGCGCACCGT ATCACCTTTT 
CCAGGCACCT GGAAACAGAG CCAGCTTGGC TATGCCACGT ACGATTCGAG CTCCTCAAGT 
CTTGGCGATT TCCAGGAGAA TTTGGCCAGC TCTCCGCCCA CCCCATGCGC GGATGTGCGA 
GCTCTTCAGC TGCGCCCCAA GTCGCTGGTG GAGAAGGCTC GTCTGAATGT GGGCTGGCTA 
GATTCCTCGC TGTCCATCAT GGAACAGGGC ATACGCGAGT ACGACACGCT GTGCCTGCGC 
TTCAAGTATT TCACTTTTTT CGATCTAAAT CCCAAGTGCG ACCAGGTGCG CATCAATCAG 
CTGTACGAGC AGGCCAAGTG GAGTGTTCTG AACGAGGAGT TGGATTGCAC AGAGGAGGAG 
AGCCTTATGT TTGCCGCCCT CCAGTTCCAG GTGAACCATC ACGTAGATCA TACACCCCAG 
GGTGCAGCAG TGGACTCGGG CATTGAAACT TCCAGTCAGG AGAATGACAA CGAAGACGAG 
ATTGATTCGG CACTGAAAGA GCTACAGATT ACGCTGGAGG GACCGGACTA CGGCGGAGAC 
TCGCGGAATA TTACACGCAT ACCAGAGCTC TCTGACTATT TGCGTTACTT GAAGCCGCAA 
AGATTCACGC TGCGCGGCTA TAAGCGCTAC TACTTCACCT ATCGGGATCT GCATTTGCAT 
CTGTTCAAGA ACGCCGAAGA TTCGCGACGT GTTGCGCCTG CCATAAGCAT TAACCTGAAG 
GGCTGCGAAG TAACTCCGGA TGTAAACCTG TCCCAGGGAA AGTATGCTAT CCGGCTGGAG 
GTCTCTCCTG ATGGTGGCCA CGGTATCAAC AGTGAGGTGT GGGTGCGCTG CGAGAACGAG 
CAGCAGTACG CCAAGTGGAT GGCTGCTTGT CGTTTGGCGG CCAAGGGAAG ATCCCTGGCG 
GATAGCTCGT ACGAGAGTGA AGTGGACAGC ATACTCTCGC TACTGCAGAT GCAGCGACCC 
GCACACGGAG TTCATGTAAA CATCGATCCT CGCTCTGTGG AAGCAGTTGA TTATCTGTCG 
CCCAAGATCA TCCGCAAGTT GTCGAACAAG GCAGTCCAGC GGATCCTCGA AGCACATGCC 
AACGTTCGGG AGCTGAATGC TTTAGATTCA AAACTGAAGT ACATCCAGGC ATGGCGTTCG 
TTACCTGACT TTGGAGTAAG CCTGTTCATC ATTAAGTTCG ACGGGCATCG GAAGGAGGAG 
CTGCTCGGCG TGGCCCACAA CCGCATTATG CGCATGGATC TGAGTTCGGG AGATCACATC 
AAGACCTGGC GATATAACAC CATGAAGGCG TGGAACGTCA ACTGGAACAT AAAGTGCATG 
ATGATTCAGT TTGAAGACGA GAATGTGGTC TTCTCCTGCC ACTCAGCCGA CTGTAAAGTG 
GTGCACGAGT TCATCGGTGG TTACATTTTC ATGTCCATGC GATCCAAGGA CACCAACCAG 
ACCCTGAACG AGGAGCTTTT CCACAAACTT ACCGGCGGCT GGTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648947.1
CDS	331..2478
Translation	MIHVGENSWNLRIFITDLALEKTMRVRGDQHIGGIMLQLVDPENPKDWSDHALWWPARNVWLSRTKLTLDQCGVQADSLLHFTPMHKILRVQLPDLRYLDSRVDYSVKTFSAVVNLCKQLDIRYPEELSLCKPLEAEHLKRNFAQVPHQKRVPIAEPDGTTYLQPAADTNSFVPISTSFHGEGGSTGSLDKPSAPGSFFCAPLSPHNHRARSPVRTVSPFPGTWKQSQLGYATYDSSSSSLGDFQENLASSPPTPCADVRALQLRPKSLVEKARLNVGWLDSSLSIMEQGIREYDTLCLRFKYFTFFDLNPKCDQVRINQLYEQAKWSVLNEELDCTEEESLMFAALQFQVNHHVDHTPQGAAVDSGIETSSQENDNEDEIDSALKELQITLEGPDYGGDSRNITRIPELSDYLRYLKPQRFTLRGYKRYYFTYRDLHLHLFKNAEDSRRVAPAISINLKGCEVTPDVNLSQGKYAIRLEVSPDGGHGINSEVWVRCENEQQYAKWMAACRLAAKGRSLADSSYESEVDSILSLLQMQRPAHGVHVNIDPRSVEAVDYLSPKIIRKLSNKAVQRILEAHANVRELNALDSKLKYIQAWRSLPDFGVSLFIIKFDGHRKEELLGVAHNRIMRMDLSSGDHIKTWRYNTMKAWNVNWNIKCMMIQFEDENVVFSCHSADCKVVHEFIGGYIFMSMRSKDTNQTLNEELFHKLTGGWS

Target ORF information:

RefSeq Version	NM_140690.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fermitin 2 (Fit2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140690.3

ORF Insert Sequence:

ATGATCCACG TCGGCGAGAA CTCGTGGAAT CTGCGGATCT TCATCACGGA TCTGGCGCTG 
GAGAAGACGA TGCGCGTGCG CGGTGACCAG CACATTGGGG GCATTATGCT GCAACTGGTG 
GATCCGGAGA ATCCCAAGGA CTGGTCGGAT CATGCTCTGT GGTGGCCAGC GCGGAATGTT 
TGGCTCAGCC GAACCAAACT TACCCTGGAT CAGTGCGGTG TCCAGGCGGA TAGCCTTCTG 
CACTTCACGC CCATGCACAA GATACTGCGG GTGCAGCTGC CGGATTTGAG GTACCTGGAC 
TCCCGGGTGG ATTACTCCGT AAAGACGTTC TCGGCGGTGG TTAATCTATG CAAGCAGTTG 
GATATCCGCT ATCCGGAGGA GTTGTCCCTG TGCAAACCAC TGGAAGCGGA GCACCTGAAG 
CGTAACTTCG CCCAGGTGCC GCATCAGAAG CGAGTGCCAA TCGCTGAACC AGATGGCACC 
ACTTACCTGC AGCCTGCTGC GGACACCAAC TCATTTGTGC CCATCAGCAC GTCGTTTCAT 
GGAGAAGGCG GAAGCACGGG CAGTCTGGAC AAGCCCTCTG CACCGGGATC CTTCTTCTGT 
GCTCCTCTGT CGCCGCACAA TCACCGAGCG CGCTCGCCAG TGCGCACCGT ATCACCTTTT 
CCAGGCACCT GGAAACAGAG CCAGCTTGGC TATGCCACGT ACGATTCGAG CTCCTCAAGT 
CTTGGCGATT TCCAGGAGAA TTTGGCCAGC TCTCCGCCCA CCCCATGCGC GGATGTGCGA 
GCTCTTCAGC TGCGCCCCAA GTCGCTGGTG GAGAAGGCTC GTCTGAATGT GGGCTGGCTA 
GATTCCTCGC TGTCCATCAT GGAACAGGGC ATACGCGAGT ACGACACGCT GTGCCTGCGC 
TTCAAGTATT TCACTTTTTT CGATCTAAAT CCCAAGTGCG ACCAGGTGCG CATCAATCAG 
CTGTACGAGC AGGCCAAGTG GAGTGTTCTG AACGAGGAGT TGGATTGCAC AGAGGAGGAG 
AGCCTTATGT TTGCCGCCCT CCAGTTCCAG GTGAACCATC ACGTAGATCA TACACCCCAG 
GGTGCAGCAG TGGACTCGGG CATTGAAACT TCCAGTCAGG AGAATGACAA CGAAGACGAG 
ATTGATTCGG CACTGAAAGA GCTACAGATT ACGCTGGAGG GACCGGACTA CGGCGGAGAC 
TCGCGGAATA TTACACGCAT ACCAGAGCTC TCTGACTATT TGCGTTACTT GAAGCCGCAA 
AGATTCACGC TGCGCGGCTA TAAGCGCTAC TACTTCACCT ATCGGGATCT GCATTTGCAT 
CTGTTCAAGA ACGCCGAAGA TTCGCGACGT GTTGCGCCTG CCATAAGCAT TAACCTGAAG 
GGCTGCGAAG TAACTCCGGA TGTAAACCTG TCCCAGGGAA AGTATGCTAT CCGGCTGGAG 
GTCTCTCCTG ATGGTGGCCA CGGTATCAAC AGTGAGGTGT GGGTGCGCTG CGAGAACGAG 
CAGCAGTACG CCAAGTGGAT GGCTGCTTGT CGTTTGGCGG CCAAGGGAAG ATCCCTGGCG 
GATAGCTCGT ACGAGAGTGA AGTGGACAGC ATACTCTCGC TACTGCAGAT GCAGCGACCC 
GCACACGGAG TTCATGTAAA CATCGATCCT CGCTCTGTGG AAGCAGTTGA TTATCTGTCG 
CCCAAGATCA TCCGCAAGTT GTCGAACAAG GCAGTCCAGC GGATCCTCGA AGCACATGCC 
AACGTTCGGG AGCTGAATGC TTTAGATTCA AAACTGAAGT ACATCCAGGC ATGGCGTTCG 
TTACCTGACT TTGGAGTAAG CCTGTTCATC ATTAAGTTCG ACGGGCATCG GAAGGAGGAG 
CTGCTCGGCG TGGCCCACAA CCGCATTATG CGCATGGATC TGAGTTCGGG AGATCACATC 
AAGACCTGGC GATATAACAC CATGAAGGCG TGGAACGTCA ACTGGAACAT AAAGTGCATG 
ATGATTCAGT TTGAAGACGA GAATGTGGTC TTCTCCTGCC ACTCAGCCGA CTGTAAAGTG 
GTGCACGAGT TCATCGGTGG TTACATTTTC ATGTCCATGC GATCCAAGGA CACCAACCAG 
ACCCTGAACG AGGAGCTTTT CCACAAACTT ACCGGCGGCT GGTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Fit2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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