Nc73EF cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nc73EF
Entrez Gene ID	39899
Full Name	Neural conserved at 73EF
Synonyms	141810_at,CG11661,Dmel\CG11661,E1,FBgn0036682,Nc73eF,anon-WO0118547.329
General protein information	Preferred Names Neural conserved at 73EF Names CG11661 gene product from transcript CG11661-RJ CG11661-PA CG11661-PB CG11661-PC CG11661-PF CG11661-PG CG11661-PH CG11661-PI CG11661-PJ Nc73EF-PA Nc73EF-PB Nc73EF-PC Nc73EF-PF Nc73EF-PG Nc73EF-PH Nc73EF-PI Nc73EF-PJ alpha-ketoglutarate dehydrogenase E1 subunit at 73EF neural conserved neural conserved at 73EF neural conserved at 73KF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 73E1-73E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_176341.3	NP_788519.1	neural conserved at 73EF, isoform G
NM_176342.5	NP_788520.2	neural conserved at 73EF, isoform H
NM_168701.5	NP_730223.1	neural conserved at 73EF, isoform A
NM_176340.3	NP_788518.1	neural conserved at 73EF, isoform F
NM_168704.4	NP_730226.3	neural conserved at 73EF, isoform J
NM_168703.5	NP_730225.1	neural conserved at 73EF, isoform C
NM_168702.2	NP_730224.1	neural conserved at 73EF, isoform B
NM_001104159.3	NP_001097629.1	neural conserved at 73EF, isoform I

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	OGDH	XP_005642239.1
Bos taurus (cattle)	OGDH	NP_001069498.1
Danio rerio (zebrafish)	ogdhb	XP_001338181.2
Danio rerio (zebrafish)	ogdha	NP_957073.2
Kluyveromyces lactis	KLLA0F04477g	XP_455282.1
Eremothecium gossypii	AGOS_AER374C	NP_985230.2
Arabidopsis thaliana (thale cress)	AT5G65750	NP_201376.1
Oryza sativa (rice)	Os04g0390000	NP_001052638.2
Homo sapiens (human)	OGDH	NP_002532.2
	OGDH	XP_001089063.1
Drosophila melanogaster (fruit fly)	Nc73EF	NP_788520.2
Saccharomyces cerevisiae (baker's yeast)	KGD1	NP_012141.1
Xenopus tropicalis (tropical clawed frog)	ogdh	NP_001135687.1
Rattus norvegicus (Norway rat)	Ogdh	NP_001017461.1
Neurospora crassa	NCU05425	XP_963248.2
Oryza sativa (rice)	Os07g0695800	NP_001060747.1
Pan troglodytes (chimpanzee)	OGDH	XP_001146811.2
Mus musculus (house mouse)	Ogdh	NP_001239211.1
Schizosaccharomyces pombe (fission yeast)	SPBC3H7.03c	NP_595772.1
Arabidopsis thaliana (thale cress)	AT3G55410	NP_191101.2
Gallus gallus (chicken)	OGDH	NP_001026553.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006366	XP_001688858.1
Caenorhabditis elegans (roundworm)	ogdh-1	NP_500617.1
Magnaporthe oryzae (rice blast fungus)	MGG_12767	XP_003716844.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nc73EF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nc73EF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18773	NM_168704.4 Latest version!	Drosophila melanogaster neural conserved at 73EF, transcript variant J (Nc73EF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa18773	NM_001104159.3 Latest version!	Drosophila melanogaster neural conserved at 73EF, transcript variant I (Nc73EF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa18774	NM_168702.2 Latest version!	Drosophila melanogaster neural conserved at 73EF, transcript variant B (Nc73EF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa18774	NM_168703.5 Latest version!	Drosophila melanogaster neural conserved at 73EF, transcript variant C (Nc73EF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa18774	NM_176341.3 Latest version!	Drosophila melanogaster neural conserved at 73EF, transcript variant G (Nc73EF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa18774	NM_168701.5 Latest version!	Drosophila melanogaster neural conserved at 73EF, transcript variant A (Nc73EF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa18775	NM_176340.3 Latest version!	Drosophila melanogaster neural conserved at 73EF, transcript variant F (Nc73EF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa18775	NM_176342.5 Latest version!	Drosophila melanogaster neural conserved at 73EF, transcript variant H (Nc73EF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nc73EF ( NM_168704.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730226.3 Drosophila melanogaster neural conserved at 73EF, transcript variant J (Nc73EF), mRNA.

Price & Availability↑

CloneID	OFa18773
Clone ID Related Accession (Same CDS sequence)	NM_168704.4 , NM_001104159.3
Accession Version	NM_168704.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neural conserved at 73EF, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_168704.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATAGAG CCCACACAGC CTTCAGCCTG GCCTTGTCGC CGATGGCGCA CAAGAACTTC 
GCCACATGGC TGCTCAAGAG CAGCAGCAGT CAGCAGATGG CCAAGGTAAC TGCAGCGGCC 
GCTGTGCGCA CCTACAACTC CGCAGCTGCC GAACCTTTTG CCAACGGCAG CACCGCCTCC 
TACGTGGAGG AGATGTACAA CGCCTGGCTT CGGGATCCCA CTTCCGTGCA CACCTCCTGG 
GACGCGTACT TCCGCAGCAA CAGCTACGTT AGCCCGCCCA ACTTGGCGCC CGTGCAGGCC 
AACACTCTGC CGCTGACCGC CTTCAACTTT GGAGGAGCCG TCGCCGGCGC TGCGCCCGAC 
TCCAAGACCA TTGACGACCA TCTGGCCGTC CAGGCCATCA TCAGGAGCTA CCAGATTCGA 
GGACATAATA TTGCTCACCT CGATCCACTT GAAATTAATA CACCAGAATT GCCTGGCAAC 
TCCTCGACGA AATCCATTTA CGCTAATTTC AGTTTTGGCG AGCAGGACAT GGATCGCCAG 
TTCAAGCTGC CCAGCACTAC CTTCATCGGA GGCGATGAGG CTTCGTTGCC CCTAAAGGAA 
ATCCTGAACC GCCTGGAGAA TGTCTACTGT AATAAGATTG GCGTGGAGTT CATGTTCATC 
AACTCTCTGG AGCAATGCAA CTGGATCCGC AAGCGTTTTG AGACCCCCGG CGTCCTTAAC 
TTCTCGCCCG AGGAGAAGCG TCTGATCCTG GCCCGTTTGA CCCGTGCCAC CGGCTTTGAG 
GCTTTCCTGG CCAAGAAGTA CTCTTCTGAG AAACGTTTCG GTCTGGAGGG TTGCGAGATC 
ATGATCCCAG CCCTGAAGGA GATCATCGAC GTATCAACGG AGTTGGGAGT GGAGTCGGTC 
ATCATGGGCA TGCCCCATCG TGGACGTCTT AACACCTTGG CCAATGTATG CCGCAAGCCC 
TTGAACCAGA TCTTCACCCA GTTCGCTGGA CTGGAGGCTG CTGATGATGG CTCTGGTGAT 
GTCAAGTACC ATCTCGGTAC ATACATCGAG CGATTGAACC GCGTTACAAA CAAGAACATC 
CGCCTGGCTG TCGTGGCCAA CCCGTCCCAT CTGGAGGCTG TGGATCCCGT GGTGCAGGGC 
AAGACCCGTG CCGAGCAGTT CTACCGTGGC GACCAGGAGG GCAAGAAGGT TATGTCCATT 
TTGATCCACG GTGATGCCGC CTTCTGCGGA CAGGGCGTCG TCTATGAAAC TATGCATCTT 
TCGGACCTGC CCGACTACAC CACCCACGGA ACTATCCATG TGGTGGCCAA CAACCAGATC 
GGTTTCACCA CCGATCCCCG TTTCTCGAGG TCCTCTCCCT ACTGTACGGA TGTGGCTCGT 
GTCGTCAATG CTCCTATTTT CCACGTCAAC GCTGACGATC CAGAGGCCGT GATGCATGTG 
TGCAAGGTGG CTGCTGAGTG GCGTGCTACT TTCCACAAGG ATTGTGTTAT TGATTTGGTC 
GGATACCGCC GCAATGGACA CAACGAGATC GACGAGCCCA TGTTCACGCA ACCCTTGATG 
TACCAGAAGA TCCGCAAGCA CAAGAACTGT CTCGACCTGT ATGCCGACAA ACTGATCGCC 
GAGGGCACTG TCACCGCCGA GGAGGTTAAG TCGGTTGCCG CTAAATACGA GAACATTTGC 
GAGGAGGCAT TCGCTCTGGC CAAGACCGAG ACCCACGTCA AGTACAAGGA CTGGCTCGAC 
TCGCCCTGGT CCGGCTTTTT CGAGGGCAAA GACCCATTGA AGGTAGCGCC CACTGGAGTG 
AAGGAGGAGA CCCTCATCCA CATCGGCAAC CGCTTCTCGT CGCCACCACC GAACGCTGCT 
GAATTCGTGA TTCACAAGGG TCTATTGCGA GTGTTGGCCG CTCGCAAGGC AATGGTGGAC 
GAAAAGGTCG CTGATTGGGC TTTGGGAGAG GCCATGGCCT TCGGCTCTCT GCTGAAGGAG 
GGAATCCATG TGCGCCTTTC GGGACAGGAT GTCGAGCGTG GTACCTTCTC GCATCGCCAC 
CATGTGCTGC ACCACCAGCT GGTCGACAAG GCCACTTACA ACTCGCTGCA GCACATGTAC 
CCAGATCAGG CGCCCTACTC CGTCTCCAAC AGCTCGCTGT CGGAGTACGC AGTGCTTGGT 
TTCGAGCACG GTTACTCGAT GACCAATCCC AATGCCCTGG TGCTGTGGGA GGCTCAGTTC 
GGAGACTTCA GCAACACGGC CCAGTCGATC ATCGATCAGT TCATCTCCAG CGGTCAGTCA 
AAGTGGGTGC GCCAATCGGG TCTGGTAATG CTCCTGCCCC ACGGCATGGA AGGCATGGGC 
CCCGAGCACT CCTCGTGCCG CGTGGAGCGC TTCCTGCAAA TGAGCAGTGA CGATCCCGAC 
TACTTCCCAC CAGAGTCCGA TGAGTTCGGA GTGCGACAGC TGCACGACAT TAACTGGATC 
GTGGCAAACT GCTCGACGCC CGCCAACTAC TACCACATCC TGCGCCGACA GATCGCATTG 
CCTTTCCGCA AGCCCCTGAT TCTGTGCACG CCCAAATCGC TGCTGCGTCA CCCCGAGGCT 
AAGAGTCCTT TCAGCGAGAT GAGCGAGGGC AGCGAGTTCC AGCGCATCAT CCCCGACAAC 
GGTCCCGCCG GCCAGAATCC CAGCAATGTG AAGAAGGTCG TCTTCTGCTC GGGCCGCGTC 
TACTACGATT TGACCAAGAC ACGTAGGGAA AAGCAGCTGG AGGGCGAAAT CGCCATTGTG 
CGCGTGGAGC AGATCTCTCC CTTCCCCTTC GATCTGGTCA AGGAGCAGGC CAACCTGTAC 
AAGAATGCCG AACTCGTGTG GGCCCAAGAG GAGCACAAGA ACCAGGGCAG CTGGACCTAT 
GTGCAACCTC GTTTCCTCAC CGCCTTGAAC CACAGCCGCG ACGTCAGCCA AAGCGATGAA 
CAATCCTCCT CCACCAATAC TACCACTACT ACTGATCATA CTAATCATGA ATCCGACACC 
GATTCCGATT CAAAATCTAA ACCGTGGCTG AGTCGCATGT TCGCCGCCCC GAACTCCTCG 
ACCGGCGGCG ATCCTAAGGA TCCGGCGCAA ACCCTTGGCG GCGACTTCAA TGCCGCCAAG 
CACTTTGACT TAAAGAATGT ACGACACGAT TTCAATAGGC CCGCGGGCAT CGCGGCCGCT 
CCGGGCGCCC GCATAGCGAA AACCGGACGC AAAATTAGCT ACGTCGGACG CGCCTGCGGA 
GCCTCCACCG CCACCGGATC CAAGGCCCAG CACATCCGTG AGCTGAATGC GCTGCTCAAC 
GACGCGATTT CGACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730226.3
CDS	460..3777
Translation	MHRAHTAFSLALSPMAHKNFATWLLKSSSSQQMAKVTAAAAVRTYNSAAAEPFANGSTASYVEEMYNAWLRDPTSVHTSWDAYFRSNSYVSPPNLAPVQANTLPLTAFNFGGAVAGAAPDSKTIDDHLAVQAIIRSYQIRGHNIAHLDPLEINTPELPGNSSTKSIYANFSFGEQDMDRQFKLPSTTFIGGDEASLPLKEILNRLENVYCNKIGVEFMFINSLEQCNWIRKRFETPGVLNFSPEEKRLILARLTRATGFEAFLAKKYSSEKRFGLEGCEIMIPALKEIIDVSTELGVESVIMGMPHRGRLNTLANVCRKPLNQIFTQFAGLEAADDGSGDVKYHLGTYIERLNRVTNKNIRLAVVANPSHLEAVDPVVQGKTRAEQFYRGDQEGKKVMSILIHGDAAFCGQGVVYETMHLSDLPDYTTHGTIHVVANNQIGFTTDPRFSRSSPYCTDVARVVNAPIFHVNADDPEAVMHVCKVAAEWRATFHKDCVIDLVGYRRNGHNEIDEPMFTQPLMYQKIRKHKNCLDLYADKLIAEGTVTAEEVKSVAAKYENICEEAFALAKTETHVKYKDWLDSPWSGFFEGKDPLKVAPTGVKEETLIHIGNRFSSPPPNAAEFVIHKGLLRVLAARKAMVDEKVADWALGEAMAFGSLLKEGIHVRLSGQDVERGTFSHRHHVLHHQLVDKATYNSLQHMYPDQAPYSVSNSSLSEYAVLGFEHGYSMTNPNALVLWEAQFGDFSNTAQSIIDQFISSGQSKWVRQSGLVMLLPHGMEGMGPEHSSCRVERFLQMSSDDPDYFPPESDEFGVRQLHDINWIVANCSTPANYYHILRRQIALPFRKPLILCTPKSLLRHPEAKSPFSEMSEGSEFQRIIPDNGPAGQNPSNVKKVVFCSGRVYYDLTKTRREKQLEGEIAIVRVEQISPFPFDLVKEQANLYKNAELVWAQEEHKNQGSWTYVQPRFLTALNHSRDVSQSDEQSSSTNTTTTTDHTNHESDTDSDSKSKPWLSRMFAAPNSSTGGDPKDPAQTLGGDFNAAKHFDLKNVRHDFNRPAGIAAAPGARIAKTGRKISYVGRACGASTATGSKAQHIRELNALLNDAIST

Target ORF information:

RefSeq Version	NM_168704.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neural conserved at 73EF, transcript variant J (Nc73EF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168704.4

ORF Insert Sequence:

ATGCATAGAG CCCACACAGC CTTCAGCCTG GCCTTGTCGC CGATGGCGCA CAAGAACTTC 
GCCACATGGC TGCTCAAGAG CAGCAGCAGT CAGCAGATGG CCAAGGTAAC TGCAGCGGCC 
GCTGTGCGCA CCTACAACTC CGCAGCTGCC GAACCTTTTG CCAACGGCAG CACCGCCTCC 
TACGTGGAGG AGATGTACAA CGCCTGGCTT CGGGATCCCA CTTCCGTGCA CACCTCCTGG 
GACGCGTACT TCCGCAGCAA CAGCTACGTT AGCCCGCCCA ACTTGGCGCC CGTGCAGGCC 
AACACTCTGC CGCTGACCGC CTTCAACTTT GGAGGAGCCG TCGCCGGCGC TGCGCCCGAC 
TCCAAGACCA TTGACGACCA TCTGGCCGTC CAGGCCATCA TCAGGAGCTA CCAGATTCGA 
GGACATAATA TTGCTCACCT CGATCCACTT GAAATTAATA CACCAGAATT GCCTGGCAAC 
TCCTCGACGA AATCCATTTA CGCTAATTTC AGTTTTGGCG AGCAGGACAT GGATCGCCAG 
TTCAAGCTGC CCAGCACTAC CTTCATCGGA GGCGATGAGG CTTCGTTGCC CCTAAAGGAA 
ATCCTGAACC GCCTGGAGAA TGTCTACTGT AATAAGATTG GCGTGGAGTT CATGTTCATC 
AACTCTCTGG AGCAATGCAA CTGGATCCGC AAGCGTTTTG AGACCCCCGG CGTCCTTAAC 
TTCTCGCCCG AGGAGAAGCG TCTGATCCTG GCCCGTTTGA CCCGTGCCAC CGGCTTTGAG 
GCTTTCCTGG CCAAGAAGTA CTCTTCTGAG AAACGTTTCG GTCTGGAGGG TTGCGAGATC 
ATGATCCCAG CCCTGAAGGA GATCATCGAC GTATCAACGG AGTTGGGAGT GGAGTCGGTC 
ATCATGGGCA TGCCCCATCG TGGACGTCTT AACACCTTGG CCAATGTATG CCGCAAGCCC 
TTGAACCAGA TCTTCACCCA GTTCGCTGGA CTGGAGGCTG CTGATGATGG CTCTGGTGAT 
GTCAAGTACC ATCTCGGTAC ATACATCGAG CGATTGAACC GCGTTACAAA CAAGAACATC 
CGCCTGGCTG TCGTGGCCAA CCCGTCCCAT CTGGAGGCTG TGGATCCCGT GGTGCAGGGC 
AAGACCCGTG CCGAGCAGTT CTACCGTGGC GACCAGGAGG GCAAGAAGGT TATGTCCATT 
TTGATCCACG GTGATGCCGC CTTCTGCGGA CAGGGCGTCG TCTATGAAAC TATGCATCTT 
TCGGACCTGC CCGACTACAC CACCCACGGA ACTATCCATG TGGTGGCCAA CAACCAGATC 
GGTTTCACCA CCGATCCCCG TTTCTCGAGG TCCTCTCCCT ACTGTACGGA TGTGGCTCGT 
GTCGTCAATG CTCCTATTTT CCACGTCAAC GCTGACGATC CAGAGGCCGT GATGCATGTG 
TGCAAGGTGG CTGCTGAGTG GCGTGCTACT TTCCACAAGG ATTGTGTTAT TGATTTGGTC 
GGATACCGCC GCAATGGACA CAACGAGATC GACGAGCCCA TGTTCACGCA ACCCTTGATG 
TACCAGAAGA TCCGCAAGCA CAAGAACTGT CTCGACCTGT ATGCCGACAA ACTGATCGCC 
GAGGGCACTG TCACCGCCGA GGAGGTTAAG TCGGTTGCCG CTAAATACGA GAACATTTGC 
GAGGAGGCAT TCGCTCTGGC CAAGACCGAG ACCCACGTCA AGTACAAGGA CTGGCTCGAC 
TCGCCCTGGT CCGGCTTTTT CGAGGGCAAA GACCCATTGA AGGTAGCGCC CACTGGAGTG 
AAGGAGGAGA CCCTCATCCA CATCGGCAAC CGCTTCTCGT CGCCACCACC GAACGCTGCT 
GAATTCGTGA TTCACAAGGG TCTATTGCGA GTGTTGGCCG CTCGCAAGGC AATGGTGGAC 
GAAAAGGTCG CTGATTGGGC TTTGGGAGAG GCCATGGCCT TCGGCTCTCT GCTGAAGGAG 
GGAATCCATG TGCGCCTTTC GGGACAGGAT GTCGAGCGTG GTACCTTCTC GCATCGCCAC 
CATGTGCTGC ACCACCAGCT GGTCGACAAG GCCACTTACA ACTCGCTGCA GCACATGTAC 
CCAGATCAGG CGCCCTACTC CGTCTCCAAC AGCTCGCTGT CGGAGTACGC AGTGCTTGGT 
TTCGAGCACG GTTACTCGAT GACCAATCCC AATGCCCTGG TGCTGTGGGA GGCTCAGTTC 
GGAGACTTCA GCAACACGGC CCAGTCGATC ATCGATCAGT TCATCTCCAG CGGTCAGTCA 
AAGTGGGTGC GCCAATCGGG TCTGGTAATG CTCCTGCCCC ACGGCATGGA AGGCATGGGC 
CCCGAGCACT CCTCGTGCCG CGTGGAGCGC TTCCTGCAAA TGAGCAGTGA CGATCCCGAC 
TACTTCCCAC CAGAGTCCGA TGAGTTCGGA GTGCGACAGC TGCACGACAT TAACTGGATC 
GTGGCAAACT GCTCGACGCC CGCCAACTAC TACCACATCC TGCGCCGACA GATCGCATTG 
CCTTTCCGCA AGCCCCTGAT TCTGTGCACG CCCAAATCGC TGCTGCGTCA CCCCGAGGCT 
AAGAGTCCTT TCAGCGAGAT GAGCGAGGGC AGCGAGTTCC AGCGCATCAT CCCCGACAAC 
GGTCCCGCCG GCCAGAATCC CAGCAATGTG AAGAAGGTCG TCTTCTGCTC GGGCCGCGTC 
TACTACGATT TGACCAAGAC ACGTAGGGAA AAGCAGCTGG AGGGCGAAAT CGCCATTGTG 
CGCGTGGAGC AGATCTCTCC CTTCCCCTTC GATCTGGTCA AGGAGCAGGC CAACCTGTAC 
AAGAATGCCG AACTCGTGTG GGCCCAAGAG GAGCACAAGA ACCAGGGCAG CTGGACCTAT 
GTGCAACCTC GTTTCCTCAC CGCCTTGAAC CACAGCCGCG ACGTCAGCCA AAGCGATGAA 
CAATCCTCCT CCACCAATAC TACCACTACT ACTGATCATA CTAATCATGA ATCCGACACC 
GATTCCGATT CAAAATCTAA ACCGTGGCTG AGTCGCATGT TCGCCGCCCC GAACTCCTCG 
ACCGGCGGCG ATCCTAAGGA TCCGGCGCAA ACCCTTGGCG GCGACTTCAA TGCCGCCAAG 
CACTTTGACT TAAAGAATGT ACGACACGAT TTCAATAGGC CCGCGGGCAT CGCGGCCGCT 
CCGGGCGCCC GCATAGCGAA AACCGGACGC AAAATTAGCT ACGTCGGACG CGCCTGCGGA 
GCCTCCACCG CCACCGGATC CAAGGCCCAG CACATCCGTG AGCTGAATGC GCTGCTCAAC 
GACGCGATTT CGACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nc73EF ( NM_001104159.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097629.1 Drosophila melanogaster neural conserved at 73EF, transcript variant I (Nc73EF), mRNA.

Price & Availability↑

CloneID	OFa18773
Clone ID Related Accession (Same CDS sequence)	NM_168704.4 , NM_001104159.3
Accession Version	NM_001104159.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neural conserved at 73EF, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001104159.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATAGAG CCCACACAGC CTTCAGCCTG GCCTTGTCGC CGATGGCGCA CAAGAACTTC 
GCCACATGGC TGCTCAAGAG CAGCAGCAGT CAGCAGATGG CCAAGGTAAC TGCAGCGGCC 
GCTGTGCGCA CCTACAACTC CGCAGCTGCC GAACCTTTTG CCAACGGCAG CACCGCCTCC 
TACGTGGAGG AGATGTACAA CGCCTGGCTT CGGGATCCCA CTTCCGTGCA CACCTCCTGG 
GACGCGTACT TCCGCAGCAA CAGCTACGTT AGCCCGCCCA ACTTGGCGCC CGTGCAGGCC 
AACACTCTGC CGCTGACCGC CTTCAACTTT GGAGGAGCCG TCGCCGGCGC TGCGCCCGAC 
TCCAAGACCA TTGACGACCA TCTGGCCGTC CAGGCCATCA TCAGGAGCTA CCAGATTCGA 
GGACATAATA TTGCTCACCT CGATCCACTT GAAATTAATA CACCAGAATT GCCTGGCAAC 
TCCTCGACGA AATCCATTTA CGCTAATTTC AGTTTTGGCG AGCAGGACAT GGATCGCCAG 
TTCAAGCTGC CCAGCACTAC CTTCATCGGA GGCGATGAGG CTTCGTTGCC CCTAAAGGAA 
ATCCTGAACC GCCTGGAGAA TGTCTACTGT AATAAGATTG GCGTGGAGTT CATGTTCATC 
AACTCTCTGG AGCAATGCAA CTGGATCCGC AAGCGTTTTG AGACCCCCGG CGTCCTTAAC 
TTCTCGCCCG AGGAGAAGCG TCTGATCCTG GCCCGTTTGA CCCGTGCCAC CGGCTTTGAG 
GCTTTCCTGG CCAAGAAGTA CTCTTCTGAG AAACGTTTCG GTCTGGAGGG TTGCGAGATC 
ATGATCCCAG CCCTGAAGGA GATCATCGAC GTATCAACGG AGTTGGGAGT GGAGTCGGTC 
ATCATGGGCA TGCCCCATCG TGGACGTCTT AACACCTTGG CCAATGTATG CCGCAAGCCC 
TTGAACCAGA TCTTCACCCA GTTCGCTGGA CTGGAGGCTG CTGATGATGG CTCTGGTGAT 
GTCAAGTACC ATCTCGGTAC ATACATCGAG CGATTGAACC GCGTTACAAA CAAGAACATC 
CGCCTGGCTG TCGTGGCCAA CCCGTCCCAT CTGGAGGCTG TGGATCCCGT GGTGCAGGGC 
AAGACCCGTG CCGAGCAGTT CTACCGTGGC GACCAGGAGG GCAAGAAGGT TATGTCCATT 
TTGATCCACG GTGATGCCGC CTTCTGCGGA CAGGGCGTCG TCTATGAAAC TATGCATCTT 
TCGGACCTGC CCGACTACAC CACCCACGGA ACTATCCATG TGGTGGCCAA CAACCAGATC 
GGTTTCACCA CCGATCCCCG TTTCTCGAGG TCCTCTCCCT ACTGTACGGA TGTGGCTCGT 
GTCGTCAATG CTCCTATTTT CCACGTCAAC GCTGACGATC CAGAGGCCGT GATGCATGTG 
TGCAAGGTGG CTGCTGAGTG GCGTGCTACT TTCCACAAGG ATTGTGTTAT TGATTTGGTC 
GGATACCGCC GCAATGGACA CAACGAGATC GACGAGCCCA TGTTCACGCA ACCCTTGATG 
TACCAGAAGA TCCGCAAGCA CAAGAACTGT CTCGACCTGT ATGCCGACAA ACTGATCGCC 
GAGGGCACTG TCACCGCCGA GGAGGTTAAG TCGGTTGCCG CTAAATACGA GAACATTTGC 
GAGGAGGCAT TCGCTCTGGC CAAGACCGAG ACCCACGTCA AGTACAAGGA CTGGCTCGAC 
TCGCCCTGGT CCGGCTTTTT CGAGGGCAAA GACCCATTGA AGGTAGCGCC CACTGGAGTG 
AAGGAGGAGA CCCTCATCCA CATCGGCAAC CGCTTCTCGT CGCCACCACC GAACGCTGCT 
GAATTCGTGA TTCACAAGGG TCTATTGCGA GTGTTGGCCG CTCGCAAGGC AATGGTGGAC 
GAAAAGGTCG CTGATTGGGC TTTGGGAGAG GCCATGGCCT TCGGCTCTCT GCTGAAGGAG 
GGAATCCATG TGCGCCTTTC GGGACAGGAT GTCGAGCGTG GTACCTTCTC GCATCGCCAC 
CATGTGCTGC ACCACCAGCT GGTCGACAAG GCCACTTACA ACTCGCTGCA GCACATGTAC 
CCAGATCAGG CGCCCTACTC CGTCTCCAAC AGCTCGCTGT CGGAGTACGC AGTGCTTGGT 
TTCGAGCACG GTTACTCGAT GACCAATCCC AATGCCCTGG TGCTGTGGGA GGCTCAGTTC 
GGAGACTTCA GCAACACGGC CCAGTCGATC ATCGATCAGT TCATCTCCAG CGGTCAGTCA 
AAGTGGGTGC GCCAATCGGG TCTGGTAATG CTCCTGCCCC ACGGCATGGA AGGCATGGGC 
CCCGAGCACT CCTCGTGCCG CGTGGAGCGC TTCCTGCAAA TGAGCAGTGA CGATCCCGAC 
TACTTCCCAC CAGAGTCCGA TGAGTTCGGA GTGCGACAGC TGCACGACAT TAACTGGATC 
GTGGCAAACT GCTCGACGCC CGCCAACTAC TACCACATCC TGCGCCGACA GATCGCATTG 
CCTTTCCGCA AGCCCCTGAT TCTGTGCACG CCCAAATCGC TGCTGCGTCA CCCCGAGGCT 
AAGAGTCCTT TCAGCGAGAT GAGCGAGGGC AGCGAGTTCC AGCGCATCAT CCCCGACAAC 
GGTCCCGCCG GCCAGAATCC CAGCAATGTG AAGAAGGTCG TCTTCTGCTC GGGCCGCGTC 
TACTACGATT TGACCAAGAC ACGTAGGGAA AAGCAGCTGG AGGGCGAAAT CGCCATTGTG 
CGCGTGGAGC AGATCTCTCC CTTCCCCTTC GATCTGGTCA AGGAGCAGGC CAACCTGTAC 
AAGAATGCCG AACTCGTGTG GGCCCAAGAG GAGCACAAGA ACCAGGGCAG CTGGACCTAT 
GTGCAACCTC GTTTCCTCAC CGCCTTGAAC CACAGCCGCG ACGTCAGCCA AAGCGATGAA 
CAATCCTCCT CCACCAATAC TACCACTACT ACTGATCATA CTAATCATGA ATCCGACACC 
GATTCCGATT CAAAATCTAA ACCGTGGCTG AGTCGCATGT TCGCCGCCCC GAACTCCTCG 
ACCGGCGGCG ATCCTAAGGA TCCGGCGCAA ACCCTTGGCG GCGACTTCAA TGCCGCCAAG 
CACTTTGACT TAAAGAATGT ACGACACGAT TTCAATAGGC CCGCGGGCAT CGCGGCCGCT 
CCGGGCGCCC GCATAGCGAA AACCGGACGC AAAATTAGCT ACGTCGGACG CGCCTGCGGA 
GCCTCCACCG CCACCGGATC CAAGGCCCAG CACATCCGTG AGCTGAATGC GCTGCTCAAC 
GACGCGATTT CGACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097629.1
CDS	112..3429
Translation	MHRAHTAFSLALSPMAHKNFATWLLKSSSSQQMAKVTAAAAVRTYNSAAAEPFANGSTASYVEEMYNAWLRDPTSVHTSWDAYFRSNSYVSPPNLAPVQANTLPLTAFNFGGAVAGAAPDSKTIDDHLAVQAIIRSYQIRGHNIAHLDPLEINTPELPGNSSTKSIYANFSFGEQDMDRQFKLPSTTFIGGDEASLPLKEILNRLENVYCNKIGVEFMFINSLEQCNWIRKRFETPGVLNFSPEEKRLILARLTRATGFEAFLAKKYSSEKRFGLEGCEIMIPALKEIIDVSTELGVESVIMGMPHRGRLNTLANVCRKPLNQIFTQFAGLEAADDGSGDVKYHLGTYIERLNRVTNKNIRLAVVANPSHLEAVDPVVQGKTRAEQFYRGDQEGKKVMSILIHGDAAFCGQGVVYETMHLSDLPDYTTHGTIHVVANNQIGFTTDPRFSRSSPYCTDVARVVNAPIFHVNADDPEAVMHVCKVAAEWRATFHKDCVIDLVGYRRNGHNEIDEPMFTQPLMYQKIRKHKNCLDLYADKLIAEGTVTAEEVKSVAAKYENICEEAFALAKTETHVKYKDWLDSPWSGFFEGKDPLKVAPTGVKEETLIHIGNRFSSPPPNAAEFVIHKGLLRVLAARKAMVDEKVADWALGEAMAFGSLLKEGIHVRLSGQDVERGTFSHRHHVLHHQLVDKATYNSLQHMYPDQAPYSVSNSSLSEYAVLGFEHGYSMTNPNALVLWEAQFGDFSNTAQSIIDQFISSGQSKWVRQSGLVMLLPHGMEGMGPEHSSCRVERFLQMSSDDPDYFPPESDEFGVRQLHDINWIVANCSTPANYYHILRRQIALPFRKPLILCTPKSLLRHPEAKSPFSEMSEGSEFQRIIPDNGPAGQNPSNVKKVVFCSGRVYYDLTKTRREKQLEGEIAIVRVEQISPFPFDLVKEQANLYKNAELVWAQEEHKNQGSWTYVQPRFLTALNHSRDVSQSDEQSSSTNTTTTTDHTNHESDTDSDSKSKPWLSRMFAAPNSSTGGDPKDPAQTLGGDFNAAKHFDLKNVRHDFNRPAGIAAAPGARIAKTGRKISYVGRACGASTATGSKAQHIRELNALLNDAIST

Target ORF information:

RefSeq Version	NM_001104159.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neural conserved at 73EF, transcript variant I (Nc73EF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104159.3

ORF Insert Sequence:

ATGCATAGAG CCCACACAGC CTTCAGCCTG GCCTTGTCGC CGATGGCGCA CAAGAACTTC 
GCCACATGGC TGCTCAAGAG CAGCAGCAGT CAGCAGATGG CCAAGGTAAC TGCAGCGGCC 
GCTGTGCGCA CCTACAACTC CGCAGCTGCC GAACCTTTTG CCAACGGCAG CACCGCCTCC 
TACGTGGAGG AGATGTACAA CGCCTGGCTT CGGGATCCCA CTTCCGTGCA CACCTCCTGG 
GACGCGTACT TCCGCAGCAA CAGCTACGTT AGCCCGCCCA ACTTGGCGCC CGTGCAGGCC 
AACACTCTGC CGCTGACCGC CTTCAACTTT GGAGGAGCCG TCGCCGGCGC TGCGCCCGAC 
TCCAAGACCA TTGACGACCA TCTGGCCGTC CAGGCCATCA TCAGGAGCTA CCAGATTCGA 
GGACATAATA TTGCTCACCT CGATCCACTT GAAATTAATA CACCAGAATT GCCTGGCAAC 
TCCTCGACGA AATCCATTTA CGCTAATTTC AGTTTTGGCG AGCAGGACAT GGATCGCCAG 
TTCAAGCTGC CCAGCACTAC CTTCATCGGA GGCGATGAGG CTTCGTTGCC CCTAAAGGAA 
ATCCTGAACC GCCTGGAGAA TGTCTACTGT AATAAGATTG GCGTGGAGTT CATGTTCATC 
AACTCTCTGG AGCAATGCAA CTGGATCCGC AAGCGTTTTG AGACCCCCGG CGTCCTTAAC 
TTCTCGCCCG AGGAGAAGCG TCTGATCCTG GCCCGTTTGA CCCGTGCCAC CGGCTTTGAG 
GCTTTCCTGG CCAAGAAGTA CTCTTCTGAG AAACGTTTCG GTCTGGAGGG TTGCGAGATC 
ATGATCCCAG CCCTGAAGGA GATCATCGAC GTATCAACGG AGTTGGGAGT GGAGTCGGTC 
ATCATGGGCA TGCCCCATCG TGGACGTCTT AACACCTTGG CCAATGTATG CCGCAAGCCC 
TTGAACCAGA TCTTCACCCA GTTCGCTGGA CTGGAGGCTG CTGATGATGG CTCTGGTGAT 
GTCAAGTACC ATCTCGGTAC ATACATCGAG CGATTGAACC GCGTTACAAA CAAGAACATC 
CGCCTGGCTG TCGTGGCCAA CCCGTCCCAT CTGGAGGCTG TGGATCCCGT GGTGCAGGGC 
AAGACCCGTG CCGAGCAGTT CTACCGTGGC GACCAGGAGG GCAAGAAGGT TATGTCCATT 
TTGATCCACG GTGATGCCGC CTTCTGCGGA CAGGGCGTCG TCTATGAAAC TATGCATCTT 
TCGGACCTGC CCGACTACAC CACCCACGGA ACTATCCATG TGGTGGCCAA CAACCAGATC 
GGTTTCACCA CCGATCCCCG TTTCTCGAGG TCCTCTCCCT ACTGTACGGA TGTGGCTCGT 
GTCGTCAATG CTCCTATTTT CCACGTCAAC GCTGACGATC CAGAGGCCGT GATGCATGTG 
TGCAAGGTGG CTGCTGAGTG GCGTGCTACT TTCCACAAGG ATTGTGTTAT TGATTTGGTC 
GGATACCGCC GCAATGGACA CAACGAGATC GACGAGCCCA TGTTCACGCA ACCCTTGATG 
TACCAGAAGA TCCGCAAGCA CAAGAACTGT CTCGACCTGT ATGCCGACAA ACTGATCGCC 
GAGGGCACTG TCACCGCCGA GGAGGTTAAG TCGGTTGCCG CTAAATACGA GAACATTTGC 
GAGGAGGCAT TCGCTCTGGC CAAGACCGAG ACCCACGTCA AGTACAAGGA CTGGCTCGAC 
TCGCCCTGGT CCGGCTTTTT CGAGGGCAAA GACCCATTGA AGGTAGCGCC CACTGGAGTG 
AAGGAGGAGA CCCTCATCCA CATCGGCAAC CGCTTCTCGT CGCCACCACC GAACGCTGCT 
GAATTCGTGA TTCACAAGGG TCTATTGCGA GTGTTGGCCG CTCGCAAGGC AATGGTGGAC 
GAAAAGGTCG CTGATTGGGC TTTGGGAGAG GCCATGGCCT TCGGCTCTCT GCTGAAGGAG 
GGAATCCATG TGCGCCTTTC GGGACAGGAT GTCGAGCGTG GTACCTTCTC GCATCGCCAC 
CATGTGCTGC ACCACCAGCT GGTCGACAAG GCCACTTACA ACTCGCTGCA GCACATGTAC 
CCAGATCAGG CGCCCTACTC CGTCTCCAAC AGCTCGCTGT CGGAGTACGC AGTGCTTGGT 
TTCGAGCACG GTTACTCGAT GACCAATCCC AATGCCCTGG TGCTGTGGGA GGCTCAGTTC 
GGAGACTTCA GCAACACGGC CCAGTCGATC ATCGATCAGT TCATCTCCAG CGGTCAGTCA 
AAGTGGGTGC GCCAATCGGG TCTGGTAATG CTCCTGCCCC ACGGCATGGA AGGCATGGGC 
CCCGAGCACT CCTCGTGCCG CGTGGAGCGC TTCCTGCAAA TGAGCAGTGA CGATCCCGAC 
TACTTCCCAC CAGAGTCCGA TGAGTTCGGA GTGCGACAGC TGCACGACAT TAACTGGATC 
GTGGCAAACT GCTCGACGCC CGCCAACTAC TACCACATCC TGCGCCGACA GATCGCATTG 
CCTTTCCGCA AGCCCCTGAT TCTGTGCACG CCCAAATCGC TGCTGCGTCA CCCCGAGGCT 
AAGAGTCCTT TCAGCGAGAT GAGCGAGGGC AGCGAGTTCC AGCGCATCAT CCCCGACAAC 
GGTCCCGCCG GCCAGAATCC CAGCAATGTG AAGAAGGTCG TCTTCTGCTC GGGCCGCGTC 
TACTACGATT TGACCAAGAC ACGTAGGGAA AAGCAGCTGG AGGGCGAAAT CGCCATTGTG 
CGCGTGGAGC AGATCTCTCC CTTCCCCTTC GATCTGGTCA AGGAGCAGGC CAACCTGTAC 
AAGAATGCCG AACTCGTGTG GGCCCAAGAG GAGCACAAGA ACCAGGGCAG CTGGACCTAT 
GTGCAACCTC GTTTCCTCAC CGCCTTGAAC CACAGCCGCG ACGTCAGCCA AAGCGATGAA 
CAATCCTCCT CCACCAATAC TACCACTACT ACTGATCATA CTAATCATGA ATCCGACACC 
GATTCCGATT CAAAATCTAA ACCGTGGCTG AGTCGCATGT TCGCCGCCCC GAACTCCTCG 
ACCGGCGGCG ATCCTAAGGA TCCGGCGCAA ACCCTTGGCG GCGACTTCAA TGCCGCCAAG 
CACTTTGACT TAAAGAATGT ACGACACGAT TTCAATAGGC CCGCGGGCAT CGCGGCCGCT 
CCGGGCGCCC GCATAGCGAA AACCGGACGC AAAATTAGCT ACGTCGGACG CGCCTGCGGA 
GCCTCCACCG CCACCGGATC CAAGGCCCAG CACATCCGTG AGCTGAATGC GCTGCTCAAC 
GACGCGATTT CGACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nc73EF ( NM_176341.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788519.1 Drosophila melanogaster neural conserved at 73EF, transcript variant G (Nc73EF), mRNA.

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CloneID	OFa18774
Clone ID Related Accession (Same CDS sequence)	NM_176341.3 , NM_168701.5 , NM_168703.5 , NM_168702.2
Accession Version	NM_176341.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neural conserved at 73EF, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_176341.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATAGAG CCCACACAGC CTTCAGCCTG GCCTTGTCGC CGATGGCGCA CAAGAACTTC 
GCCACATGGC TGCTCAAGAG CAGCAGCAGT CAGCAGATGG CCAAGGTAAC TGCAGCGGCC 
GCTGTGCGCA CCTACAACTC CGCAGCTGCC GAACCTTTTG CCAACGGCAG CACCGCCTCC 
TACGTGGAGG AGATGTACAA CGCCTGGCTT CGGGATCCCA CTTCCGTGCA CACCTCCTGG 
GACGCGTACT TCCGCAGCAA CAGCTACGTT AGCCCGCCCA ACTTGGCGCC CGTGCAGGCC 
AACACTCTGC CGCTGACCGC CTTCAACTTT GGAGGAGCCG TCGCCGGCGC TGCGCCCGAC 
TCCAAGACCA TTGACGACCA TCTGGCCGTC CAGGCCATCA TCAGGAGCTA CCAGATTCGA 
GGACATAATA TTGCTCACCT CGATCCACTT GAAATTAATA CACCAGAATT GCCTGGCAAC 
TCCTCGACGA AATCCATTTA CGCTAATTTC AGTTTTGGCG AGCAGGACAT GGATCGCCAG 
TTCAAGCTGC CCAGCACTAC CTTCATCGGA GGCGATGAGG CTTCGTTGCC CCTAAAGGAA 
ATCCTGAACC GCCTGGAGAA TGTCTACTGT AATAAGATTG GCGTGGAGTT CATGTTCATC 
AACTCTCTGG AGCAATGCAA CTGGATCCGC AAGCGTTTTG AGACCCCCGG CGTCCTTAAC 
TTCTCGCCCG AGGAGAAGCG TCTGATCCTG GCCCGTTTGA CCCGTGCCAC CGGCTTTGAG 
GCTTTCCTGG CCAAGAAGTA CTCTTCTGAG AAACGTTTCG GTCTGGAGGG TTGCGAGATC 
ATGATCCCAG CCCTGAAGGA GATCATCGAC GTATCAACGG AGTTGGGAGT GGAGTCGGTC 
ATCATGGGCA TGCCCCATCG TGGACGTCTT AACACCTTGG CCAATGTATG CCGCAAGCCC 
TTGAACCAGA TCTTCACCCA GTTCGCTGGA CTGGAGGCTG CTGATGATGG CTCTGGTGAT 
GTCAAGTACC ATCTCGGTAC ATACATCGAG CGATTGAACC GCGTTACAAA CAAGAACATC 
CGCCTGGCTG TCGTGGCCAA CCCGTCCCAT CTGGAGGCTG TGGATCCCGT GGTGCAGGGC 
AAGACCCGTG CCGAGCAGTT CTACCGTGGC GACCAGGAGG GCAAGAAGGT TATGTCCATT 
TTGATCCACG GTGATGCCGC CTTCTGCGGA CAGGGCGTCG TCTATGAAAC TATGCATCTT 
TCGGACCTGC CCGACTACAC CACCCACGGA ACTATCCATG TGGTGGCCAA CAACCAGATC 
GGTTTCACCA CCGATCCCCG TTTCTCGAGG TCCTCTCCCT ACTGTACGGA TGTGGCTCGT 
GTCGTCAATG CTCCTATTTT CCACGTCAAC GCTGACGATC CAGAGGCCGT GATGCATGTG 
TGCAAGGTGG CTGCTGAGTG GCGTGCTACT TTCCACAAGG ATTGTGTTAT TGATTTGGTC 
GGATACCGCC GCAATGGACA CAACGAGATC GACGAGCCCA TGTTCACGCA ACCCTTGATG 
TACCAGAAGA TCCGCAAGCA CAAGAACTGT CTCGACCTGT ATGCCGACAA ACTGATCGCC 
GAGGGCACTG TCACCGCCGA GGAGGTTAAG TCGGTTGCCG CTAAATACGA GAACATTTGC 
GAGGAGGCAT TCGCTCTGGC CAAGACCGAG ACCCACGTCA AGTACAAGGA CTGGCTCGAC 
TCGCCCTGGT CCGGCTTTTT CGAGGGCAAA GACCCATTGA AGGTAGCGCC CACTGGAGTG 
AAGGAGGAGA CCCTCATCCA CATCGGCAAC CGCTTCTCGT CGCCACCACC GAACGCTGCT 
GAATTCGTGA TTCACAAGGG TCTATTGCGA GTGTTGGCCG CTCGCAAGGC AATGGTGGAC 
GAAAAGGTCG CTGATTGGGC TTTGGGAGAG GCCATGGCCT TCGGCTCTCT GCTGAAGGAG 
GGAATCCATG TGCGCCTTTC GGGACAGGAT GTCGAGCGTG GTACCTTCTC GCATCGCCAC 
CATGTGCTGC ACCACCAGCT GGTCGACAAG GCCACTTACA ACTCGCTGCA GCACATGTAC 
CCAGATCAGG CGCCCTACTC CGTCTCCAAC AGCTCGCTGT CGGAGTACGC AGTGCTTGGT 
TTCGAGCACG GTTACTCGAT GACCAATCCC AATGCCCTGG TGCTGTGGGA GGCTCAGTTC 
GGAGACTTCA GCAACACGGC CCAGTCGATC ATCGATCAGT TCATCTCCAG CGGTCAGTCA 
AAGTGGGTGC GCCAATCGGG TCTGGTAATG CTCCTGCCCC ACGGCATGGA AGGCATGGGC 
CCCGAGCACT CCTCGTGCCG CGTGGAGCGC TTCCTGCAAA TGAGCAGTGA CGATCCCGAC 
TACTTCCCAC CAGAGTCCGA TGAGTTCGGA GTGCGACAGC TGCACGACAT TAACTGGATC 
GTGGCAAACT GCTCGACGCC CGCCAACTAC TACCACATCC TGCGCCGACA GATCGCATTG 
CCTTTCCGCA AGCCCCTGAT TCTGTGCACG CCCAAATCGC TGCTGCGTCA CCCCGAGGCT 
AAGAGTCCTT TCAGCGAGAT GAGCGAGGGC AGCGAGTTCC AGCGCATCAT CCCCGACAAC 
GGTCCCGCCG GCCAGAATCC CAGCAATGTG AAGAAGGTCG TCTTCTGCTC GGGCCGCGTC 
TACTACGATT TGACCAAGAC ACGTAGGGAA AAGCAGCTGG AGGGCGAAAT CGCCATTGTG 
CGCGTGGAGC AGATCTCTCC CTTCCCCTTC GATCTGGTCA AGGAGCAGGC CAACCTGTAC 
AAGAATGCCG AACTCGTGTG GGCCCAAGAG GAGCACAAGA ACCAGGGCAG CTGGACCTAT 
GTGCAACCTC GTTTCCTCAC CGCCTTGAAC CACAGCCGCG ACGTCAGCTA CGTCGGACGC 
GCCTGCGGAG CCTCCACCGC CACCGGATCC AAGGCCCAGC ACATCCGTGA GCTGAATGCG 
CTGCTCAACG ACGCGATTTC GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788519.1
CDS	66..3092
Translation	MHRAHTAFSLALSPMAHKNFATWLLKSSSSQQMAKVTAAAAVRTYNSAAAEPFANGSTASYVEEMYNAWLRDPTSVHTSWDAYFRSNSYVSPPNLAPVQANTLPLTAFNFGGAVAGAAPDSKTIDDHLAVQAIIRSYQIRGHNIAHLDPLEINTPELPGNSSTKSIYANFSFGEQDMDRQFKLPSTTFIGGDEASLPLKEILNRLENVYCNKIGVEFMFINSLEQCNWIRKRFETPGVLNFSPEEKRLILARLTRATGFEAFLAKKYSSEKRFGLEGCEIMIPALKEIIDVSTELGVESVIMGMPHRGRLNTLANVCRKPLNQIFTQFAGLEAADDGSGDVKYHLGTYIERLNRVTNKNIRLAVVANPSHLEAVDPVVQGKTRAEQFYRGDQEGKKVMSILIHGDAAFCGQGVVYETMHLSDLPDYTTHGTIHVVANNQIGFTTDPRFSRSSPYCTDVARVVNAPIFHVNADDPEAVMHVCKVAAEWRATFHKDCVIDLVGYRRNGHNEIDEPMFTQPLMYQKIRKHKNCLDLYADKLIAEGTVTAEEVKSVAAKYENICEEAFALAKTETHVKYKDWLDSPWSGFFEGKDPLKVAPTGVKEETLIHIGNRFSSPPPNAAEFVIHKGLLRVLAARKAMVDEKVADWALGEAMAFGSLLKEGIHVRLSGQDVERGTFSHRHHVLHHQLVDKATYNSLQHMYPDQAPYSVSNSSLSEYAVLGFEHGYSMTNPNALVLWEAQFGDFSNTAQSIIDQFISSGQSKWVRQSGLVMLLPHGMEGMGPEHSSCRVERFLQMSSDDPDYFPPESDEFGVRQLHDINWIVANCSTPANYYHILRRQIALPFRKPLILCTPKSLLRHPEAKSPFSEMSEGSEFQRIIPDNGPAGQNPSNVKKVVFCSGRVYYDLTKTRREKQLEGEIAIVRVEQISPFPFDLVKEQANLYKNAELVWAQEEHKNQGSWTYVQPRFLTALNHSRDVSYVGRACGASTATGSKAQHIRELNALLNDAIST

Target ORF information:

RefSeq Version	NM_176341.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neural conserved at 73EF, transcript variant G (Nc73EF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176341.3

ORF Insert Sequence:

ATGCATAGAG CCCACACAGC CTTCAGCCTG GCCTTGTCGC CGATGGCGCA CAAGAACTTC 
GCCACATGGC TGCTCAAGAG CAGCAGCAGT CAGCAGATGG CCAAGGTAAC TGCAGCGGCC 
GCTGTGCGCA CCTACAACTC CGCAGCTGCC GAACCTTTTG CCAACGGCAG CACCGCCTCC 
TACGTGGAGG AGATGTACAA CGCCTGGCTT CGGGATCCCA CTTCCGTGCA CACCTCCTGG 
GACGCGTACT TCCGCAGCAA CAGCTACGTT AGCCCGCCCA ACTTGGCGCC CGTGCAGGCC 
AACACTCTGC CGCTGACCGC CTTCAACTTT GGAGGAGCCG TCGCCGGCGC TGCGCCCGAC 
TCCAAGACCA TTGACGACCA TCTGGCCGTC CAGGCCATCA TCAGGAGCTA CCAGATTCGA 
GGACATAATA TTGCTCACCT CGATCCACTT GAAATTAATA CACCAGAATT GCCTGGCAAC 
TCCTCGACGA AATCCATTTA CGCTAATTTC AGTTTTGGCG AGCAGGACAT GGATCGCCAG 
TTCAAGCTGC CCAGCACTAC CTTCATCGGA GGCGATGAGG CTTCGTTGCC CCTAAAGGAA 
ATCCTGAACC GCCTGGAGAA TGTCTACTGT AATAAGATTG GCGTGGAGTT CATGTTCATC 
AACTCTCTGG AGCAATGCAA CTGGATCCGC AAGCGTTTTG AGACCCCCGG CGTCCTTAAC 
TTCTCGCCCG AGGAGAAGCG TCTGATCCTG GCCCGTTTGA CCCGTGCCAC CGGCTTTGAG 
GCTTTCCTGG CCAAGAAGTA CTCTTCTGAG AAACGTTTCG GTCTGGAGGG TTGCGAGATC 
ATGATCCCAG CCCTGAAGGA GATCATCGAC GTATCAACGG AGTTGGGAGT GGAGTCGGTC 
ATCATGGGCA TGCCCCATCG TGGACGTCTT AACACCTTGG CCAATGTATG CCGCAAGCCC 
TTGAACCAGA TCTTCACCCA GTTCGCTGGA CTGGAGGCTG CTGATGATGG CTCTGGTGAT 
GTCAAGTACC ATCTCGGTAC ATACATCGAG CGATTGAACC GCGTTACAAA CAAGAACATC 
CGCCTGGCTG TCGTGGCCAA CCCGTCCCAT CTGGAGGCTG TGGATCCCGT GGTGCAGGGC 
AAGACCCGTG CCGAGCAGTT CTACCGTGGC GACCAGGAGG GCAAGAAGGT TATGTCCATT 
TTGATCCACG GTGATGCCGC CTTCTGCGGA CAGGGCGTCG TCTATGAAAC TATGCATCTT 
TCGGACCTGC CCGACTACAC CACCCACGGA ACTATCCATG TGGTGGCCAA CAACCAGATC 
GGTTTCACCA CCGATCCCCG TTTCTCGAGG TCCTCTCCCT ACTGTACGGA TGTGGCTCGT 
GTCGTCAATG CTCCTATTTT CCACGTCAAC GCTGACGATC CAGAGGCCGT GATGCATGTG 
TGCAAGGTGG CTGCTGAGTG GCGTGCTACT TTCCACAAGG ATTGTGTTAT TGATTTGGTC 
GGATACCGCC GCAATGGACA CAACGAGATC GACGAGCCCA TGTTCACGCA ACCCTTGATG 
TACCAGAAGA TCCGCAAGCA CAAGAACTGT CTCGACCTGT ATGCCGACAA ACTGATCGCC 
GAGGGCACTG TCACCGCCGA GGAGGTTAAG TCGGTTGCCG CTAAATACGA GAACATTTGC 
GAGGAGGCAT TCGCTCTGGC CAAGACCGAG ACCCACGTCA AGTACAAGGA CTGGCTCGAC 
TCGCCCTGGT CCGGCTTTTT CGAGGGCAAA GACCCATTGA AGGTAGCGCC CACTGGAGTG 
AAGGAGGAGA CCCTCATCCA CATCGGCAAC CGCTTCTCGT CGCCACCACC GAACGCTGCT 
GAATTCGTGA TTCACAAGGG TCTATTGCGA GTGTTGGCCG CTCGCAAGGC AATGGTGGAC 
GAAAAGGTCG CTGATTGGGC TTTGGGAGAG GCCATGGCCT TCGGCTCTCT GCTGAAGGAG 
GGAATCCATG TGCGCCTTTC GGGACAGGAT GTCGAGCGTG GTACCTTCTC GCATCGCCAC 
CATGTGCTGC ACCACCAGCT GGTCGACAAG GCCACTTACA ACTCGCTGCA GCACATGTAC 
CCAGATCAGG CGCCCTACTC CGTCTCCAAC AGCTCGCTGT CGGAGTACGC AGTGCTTGGT 
TTCGAGCACG GTTACTCGAT GACCAATCCC AATGCCCTGG TGCTGTGGGA GGCTCAGTTC 
GGAGACTTCA GCAACACGGC CCAGTCGATC ATCGATCAGT TCATCTCCAG CGGTCAGTCA 
AAGTGGGTGC GCCAATCGGG TCTGGTAATG CTCCTGCCCC ACGGCATGGA AGGCATGGGC 
CCCGAGCACT CCTCGTGCCG CGTGGAGCGC TTCCTGCAAA TGAGCAGTGA CGATCCCGAC 
TACTTCCCAC CAGAGTCCGA TGAGTTCGGA GTGCGACAGC TGCACGACAT TAACTGGATC 
GTGGCAAACT GCTCGACGCC CGCCAACTAC TACCACATCC TGCGCCGACA GATCGCATTG 
CCTTTCCGCA AGCCCCTGAT TCTGTGCACG CCCAAATCGC TGCTGCGTCA CCCCGAGGCT 
AAGAGTCCTT TCAGCGAGAT GAGCGAGGGC AGCGAGTTCC AGCGCATCAT CCCCGACAAC 
GGTCCCGCCG GCCAGAATCC CAGCAATGTG AAGAAGGTCG TCTTCTGCTC GGGCCGCGTC 
TACTACGATT TGACCAAGAC ACGTAGGGAA AAGCAGCTGG AGGGCGAAAT CGCCATTGTG 
CGCGTGGAGC AGATCTCTCC CTTCCCCTTC GATCTGGTCA AGGAGCAGGC CAACCTGTAC 
AAGAATGCCG AACTCGTGTG GGCCCAAGAG GAGCACAAGA ACCAGGGCAG CTGGACCTAT 
GTGCAACCTC GTTTCCTCAC CGCCTTGAAC CACAGCCGCG ACGTCAGCTA CGTCGGACGC 
GCCTGCGGAG CCTCCACCGC CACCGGATCC AAGGCCCAGC ACATCCGTGA GCTGAATGCG 
CTGCTCAACG ACGCGATTTC GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nc73EF ( NM_168701.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730223.1 Drosophila melanogaster neural conserved at 73EF, transcript variant A (Nc73EF), mRNA.

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CloneID	OFa18774
Clone ID Related Accession (Same CDS sequence)	NM_176341.3 , NM_168701.5 , NM_168703.5 , NM_168702.2
Accession Version	NM_168701.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neural conserved at 73EF, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_168701.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATAGAG CCCACACAGC CTTCAGCCTG GCCTTGTCGC CGATGGCGCA CAAGAACTTC 
GCCACATGGC TGCTCAAGAG CAGCAGCAGT CAGCAGATGG CCAAGGTAAC TGCAGCGGCC 
GCTGTGCGCA CCTACAACTC CGCAGCTGCC GAACCTTTTG CCAACGGCAG CACCGCCTCC 
TACGTGGAGG AGATGTACAA CGCCTGGCTT CGGGATCCCA CTTCCGTGCA CACCTCCTGG 
GACGCGTACT TCCGCAGCAA CAGCTACGTT AGCCCGCCCA ACTTGGCGCC CGTGCAGGCC 
AACACTCTGC CGCTGACCGC CTTCAACTTT GGAGGAGCCG TCGCCGGCGC TGCGCCCGAC 
TCCAAGACCA TTGACGACCA TCTGGCCGTC CAGGCCATCA TCAGGAGCTA CCAGATTCGA 
GGACATAATA TTGCTCACCT CGATCCACTT GAAATTAATA CACCAGAATT GCCTGGCAAC 
TCCTCGACGA AATCCATTTA CGCTAATTTC AGTTTTGGCG AGCAGGACAT GGATCGCCAG 
TTCAAGCTGC CCAGCACTAC CTTCATCGGA GGCGATGAGG CTTCGTTGCC CCTAAAGGAA 
ATCCTGAACC GCCTGGAGAA TGTCTACTGT AATAAGATTG GCGTGGAGTT CATGTTCATC 
AACTCTCTGG AGCAATGCAA CTGGATCCGC AAGCGTTTTG AGACCCCCGG CGTCCTTAAC 
TTCTCGCCCG AGGAGAAGCG TCTGATCCTG GCCCGTTTGA CCCGTGCCAC CGGCTTTGAG 
GCTTTCCTGG CCAAGAAGTA CTCTTCTGAG AAACGTTTCG GTCTGGAGGG TTGCGAGATC 
ATGATCCCAG CCCTGAAGGA GATCATCGAC GTATCAACGG AGTTGGGAGT GGAGTCGGTC 
ATCATGGGCA TGCCCCATCG TGGACGTCTT AACACCTTGG CCAATGTATG CCGCAAGCCC 
TTGAACCAGA TCTTCACCCA GTTCGCTGGA CTGGAGGCTG CTGATGATGG CTCTGGTGAT 
GTCAAGTACC ATCTCGGTAC ATACATCGAG CGATTGAACC GCGTTACAAA CAAGAACATC 
CGCCTGGCTG TCGTGGCCAA CCCGTCCCAT CTGGAGGCTG TGGATCCCGT GGTGCAGGGC 
AAGACCCGTG CCGAGCAGTT CTACCGTGGC GACCAGGAGG GCAAGAAGGT TATGTCCATT 
TTGATCCACG GTGATGCCGC CTTCTGCGGA CAGGGCGTCG TCTATGAAAC TATGCATCTT 
TCGGACCTGC CCGACTACAC CACCCACGGA ACTATCCATG TGGTGGCCAA CAACCAGATC 
GGTTTCACCA CCGATCCCCG TTTCTCGAGG TCCTCTCCCT ACTGTACGGA TGTGGCTCGT 
GTCGTCAATG CTCCTATTTT CCACGTCAAC GCTGACGATC CAGAGGCCGT GATGCATGTG 
TGCAAGGTGG CTGCTGAGTG GCGTGCTACT TTCCACAAGG ATTGTGTTAT TGATTTGGTC 
GGATACCGCC GCAATGGACA CAACGAGATC GACGAGCCCA TGTTCACGCA ACCCTTGATG 
TACCAGAAGA TCCGCAAGCA CAAGAACTGT CTCGACCTGT ATGCCGACAA ACTGATCGCC 
GAGGGCACTG TCACCGCCGA GGAGGTTAAG TCGGTTGCCG CTAAATACGA GAACATTTGC 
GAGGAGGCAT TCGCTCTGGC CAAGACCGAG ACCCACGTCA AGTACAAGGA CTGGCTCGAC 
TCGCCCTGGT CCGGCTTTTT CGAGGGCAAA GACCCATTGA AGGTAGCGCC CACTGGAGTG 
AAGGAGGAGA CCCTCATCCA CATCGGCAAC CGCTTCTCGT CGCCACCACC GAACGCTGCT 
GAATTCGTGA TTCACAAGGG TCTATTGCGA GTGTTGGCCG CTCGCAAGGC AATGGTGGAC 
GAAAAGGTCG CTGATTGGGC TTTGGGAGAG GCCATGGCCT TCGGCTCTCT GCTGAAGGAG 
GGAATCCATG TGCGCCTTTC GGGACAGGAT GTCGAGCGTG GTACCTTCTC GCATCGCCAC 
CATGTGCTGC ACCACCAGCT GGTCGACAAG GCCACTTACA ACTCGCTGCA GCACATGTAC 
CCAGATCAGG CGCCCTACTC CGTCTCCAAC AGCTCGCTGT CGGAGTACGC AGTGCTTGGT 
TTCGAGCACG GTTACTCGAT GACCAATCCC AATGCCCTGG TGCTGTGGGA GGCTCAGTTC 
GGAGACTTCA GCAACACGGC CCAGTCGATC ATCGATCAGT TCATCTCCAG CGGTCAGTCA 
AAGTGGGTGC GCCAATCGGG TCTGGTAATG CTCCTGCCCC ACGGCATGGA AGGCATGGGC 
CCCGAGCACT CCTCGTGCCG CGTGGAGCGC TTCCTGCAAA TGAGCAGTGA CGATCCCGAC 
TACTTCCCAC CAGAGTCCGA TGAGTTCGGA GTGCGACAGC TGCACGACAT TAACTGGATC 
GTGGCAAACT GCTCGACGCC CGCCAACTAC TACCACATCC TGCGCCGACA GATCGCATTG 
CCTTTCCGCA AGCCCCTGAT TCTGTGCACG CCCAAATCGC TGCTGCGTCA CCCCGAGGCT 
AAGAGTCCTT TCAGCGAGAT GAGCGAGGGC AGCGAGTTCC AGCGCATCAT CCCCGACAAC 
GGTCCCGCCG GCCAGAATCC CAGCAATGTG AAGAAGGTCG TCTTCTGCTC GGGCCGCGTC 
TACTACGATT TGACCAAGAC ACGTAGGGAA AAGCAGCTGG AGGGCGAAAT CGCCATTGTG 
CGCGTGGAGC AGATCTCTCC CTTCCCCTTC GATCTGGTCA AGGAGCAGGC CAACCTGTAC 
AAGAATGCCG AACTCGTGTG GGCCCAAGAG GAGCACAAGA ACCAGGGCAG CTGGACCTAT 
GTGCAACCTC GTTTCCTCAC CGCCTTGAAC CACAGCCGCG ACGTCAGCTA CGTCGGACGC 
GCCTGCGGAG CCTCCACCGC CACCGGATCC AAGGCCCAGC ACATCCGTGA GCTGAATGCG 
CTGCTCAACG ACGCGATTTC GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730223.1
CDS	112..3138
Translation	MHRAHTAFSLALSPMAHKNFATWLLKSSSSQQMAKVTAAAAVRTYNSAAAEPFANGSTASYVEEMYNAWLRDPTSVHTSWDAYFRSNSYVSPPNLAPVQANTLPLTAFNFGGAVAGAAPDSKTIDDHLAVQAIIRSYQIRGHNIAHLDPLEINTPELPGNSSTKSIYANFSFGEQDMDRQFKLPSTTFIGGDEASLPLKEILNRLENVYCNKIGVEFMFINSLEQCNWIRKRFETPGVLNFSPEEKRLILARLTRATGFEAFLAKKYSSEKRFGLEGCEIMIPALKEIIDVSTELGVESVIMGMPHRGRLNTLANVCRKPLNQIFTQFAGLEAADDGSGDVKYHLGTYIERLNRVTNKNIRLAVVANPSHLEAVDPVVQGKTRAEQFYRGDQEGKKVMSILIHGDAAFCGQGVVYETMHLSDLPDYTTHGTIHVVANNQIGFTTDPRFSRSSPYCTDVARVVNAPIFHVNADDPEAVMHVCKVAAEWRATFHKDCVIDLVGYRRNGHNEIDEPMFTQPLMYQKIRKHKNCLDLYADKLIAEGTVTAEEVKSVAAKYENICEEAFALAKTETHVKYKDWLDSPWSGFFEGKDPLKVAPTGVKEETLIHIGNRFSSPPPNAAEFVIHKGLLRVLAARKAMVDEKVADWALGEAMAFGSLLKEGIHVRLSGQDVERGTFSHRHHVLHHQLVDKATYNSLQHMYPDQAPYSVSNSSLSEYAVLGFEHGYSMTNPNALVLWEAQFGDFSNTAQSIIDQFISSGQSKWVRQSGLVMLLPHGMEGMGPEHSSCRVERFLQMSSDDPDYFPPESDEFGVRQLHDINWIVANCSTPANYYHILRRQIALPFRKPLILCTPKSLLRHPEAKSPFSEMSEGSEFQRIIPDNGPAGQNPSNVKKVVFCSGRVYYDLTKTRREKQLEGEIAIVRVEQISPFPFDLVKEQANLYKNAELVWAQEEHKNQGSWTYVQPRFLTALNHSRDVSYVGRACGASTATGSKAQHIRELNALLNDAIST

Target ORF information:

RefSeq Version	NM_168701.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neural conserved at 73EF, transcript variant A (Nc73EF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168701.5

ORF Insert Sequence:

ATGCATAGAG CCCACACAGC CTTCAGCCTG GCCTTGTCGC CGATGGCGCA CAAGAACTTC 
GCCACATGGC TGCTCAAGAG CAGCAGCAGT CAGCAGATGG CCAAGGTAAC TGCAGCGGCC 
GCTGTGCGCA CCTACAACTC CGCAGCTGCC GAACCTTTTG CCAACGGCAG CACCGCCTCC 
TACGTGGAGG AGATGTACAA CGCCTGGCTT CGGGATCCCA CTTCCGTGCA CACCTCCTGG 
GACGCGTACT TCCGCAGCAA CAGCTACGTT AGCCCGCCCA ACTTGGCGCC CGTGCAGGCC 
AACACTCTGC CGCTGACCGC CTTCAACTTT GGAGGAGCCG TCGCCGGCGC TGCGCCCGAC 
TCCAAGACCA TTGACGACCA TCTGGCCGTC CAGGCCATCA TCAGGAGCTA CCAGATTCGA 
GGACATAATA TTGCTCACCT CGATCCACTT GAAATTAATA CACCAGAATT GCCTGGCAAC 
TCCTCGACGA AATCCATTTA CGCTAATTTC AGTTTTGGCG AGCAGGACAT GGATCGCCAG 
TTCAAGCTGC CCAGCACTAC CTTCATCGGA GGCGATGAGG CTTCGTTGCC CCTAAAGGAA 
ATCCTGAACC GCCTGGAGAA TGTCTACTGT AATAAGATTG GCGTGGAGTT CATGTTCATC 
AACTCTCTGG AGCAATGCAA CTGGATCCGC AAGCGTTTTG AGACCCCCGG CGTCCTTAAC 
TTCTCGCCCG AGGAGAAGCG TCTGATCCTG GCCCGTTTGA CCCGTGCCAC CGGCTTTGAG 
GCTTTCCTGG CCAAGAAGTA CTCTTCTGAG AAACGTTTCG GTCTGGAGGG TTGCGAGATC 
ATGATCCCAG CCCTGAAGGA GATCATCGAC GTATCAACGG AGTTGGGAGT GGAGTCGGTC 
ATCATGGGCA TGCCCCATCG TGGACGTCTT AACACCTTGG CCAATGTATG CCGCAAGCCC 
TTGAACCAGA TCTTCACCCA GTTCGCTGGA CTGGAGGCTG CTGATGATGG CTCTGGTGAT 
GTCAAGTACC ATCTCGGTAC ATACATCGAG CGATTGAACC GCGTTACAAA CAAGAACATC 
CGCCTGGCTG TCGTGGCCAA CCCGTCCCAT CTGGAGGCTG TGGATCCCGT GGTGCAGGGC 
AAGACCCGTG CCGAGCAGTT CTACCGTGGC GACCAGGAGG GCAAGAAGGT TATGTCCATT 
TTGATCCACG GTGATGCCGC CTTCTGCGGA CAGGGCGTCG TCTATGAAAC TATGCATCTT 
TCGGACCTGC CCGACTACAC CACCCACGGA ACTATCCATG TGGTGGCCAA CAACCAGATC 
GGTTTCACCA CCGATCCCCG TTTCTCGAGG TCCTCTCCCT ACTGTACGGA TGTGGCTCGT 
GTCGTCAATG CTCCTATTTT CCACGTCAAC GCTGACGATC CAGAGGCCGT GATGCATGTG 
TGCAAGGTGG CTGCTGAGTG GCGTGCTACT TTCCACAAGG ATTGTGTTAT TGATTTGGTC 
GGATACCGCC GCAATGGACA CAACGAGATC GACGAGCCCA TGTTCACGCA ACCCTTGATG 
TACCAGAAGA TCCGCAAGCA CAAGAACTGT CTCGACCTGT ATGCCGACAA ACTGATCGCC 
GAGGGCACTG TCACCGCCGA GGAGGTTAAG TCGGTTGCCG CTAAATACGA GAACATTTGC 
GAGGAGGCAT TCGCTCTGGC CAAGACCGAG ACCCACGTCA AGTACAAGGA CTGGCTCGAC 
TCGCCCTGGT CCGGCTTTTT CGAGGGCAAA GACCCATTGA AGGTAGCGCC CACTGGAGTG 
AAGGAGGAGA CCCTCATCCA CATCGGCAAC CGCTTCTCGT CGCCACCACC GAACGCTGCT 
GAATTCGTGA TTCACAAGGG TCTATTGCGA GTGTTGGCCG CTCGCAAGGC AATGGTGGAC 
GAAAAGGTCG CTGATTGGGC TTTGGGAGAG GCCATGGCCT TCGGCTCTCT GCTGAAGGAG 
GGAATCCATG TGCGCCTTTC GGGACAGGAT GTCGAGCGTG GTACCTTCTC GCATCGCCAC 
CATGTGCTGC ACCACCAGCT GGTCGACAAG GCCACTTACA ACTCGCTGCA GCACATGTAC 
CCAGATCAGG CGCCCTACTC CGTCTCCAAC AGCTCGCTGT CGGAGTACGC AGTGCTTGGT 
TTCGAGCACG GTTACTCGAT GACCAATCCC AATGCCCTGG TGCTGTGGGA GGCTCAGTTC 
GGAGACTTCA GCAACACGGC CCAGTCGATC ATCGATCAGT TCATCTCCAG CGGTCAGTCA 
AAGTGGGTGC GCCAATCGGG TCTGGTAATG CTCCTGCCCC ACGGCATGGA AGGCATGGGC 
CCCGAGCACT CCTCGTGCCG CGTGGAGCGC TTCCTGCAAA TGAGCAGTGA CGATCCCGAC 
TACTTCCCAC CAGAGTCCGA TGAGTTCGGA GTGCGACAGC TGCACGACAT TAACTGGATC 
GTGGCAAACT GCTCGACGCC CGCCAACTAC TACCACATCC TGCGCCGACA GATCGCATTG 
CCTTTCCGCA AGCCCCTGAT TCTGTGCACG CCCAAATCGC TGCTGCGTCA CCCCGAGGCT 
AAGAGTCCTT TCAGCGAGAT GAGCGAGGGC AGCGAGTTCC AGCGCATCAT CCCCGACAAC 
GGTCCCGCCG GCCAGAATCC CAGCAATGTG AAGAAGGTCG TCTTCTGCTC GGGCCGCGTC 
TACTACGATT TGACCAAGAC ACGTAGGGAA AAGCAGCTGG AGGGCGAAAT CGCCATTGTG 
CGCGTGGAGC AGATCTCTCC CTTCCCCTTC GATCTGGTCA AGGAGCAGGC CAACCTGTAC 
AAGAATGCCG AACTCGTGTG GGCCCAAGAG GAGCACAAGA ACCAGGGCAG CTGGACCTAT 
GTGCAACCTC GTTTCCTCAC CGCCTTGAAC CACAGCCGCG ACGTCAGCTA CGTCGGACGC 
GCCTGCGGAG CCTCCACCGC CACCGGATCC AAGGCCCAGC ACATCCGTGA GCTGAATGCG 
CTGCTCAACG ACGCGATTTC GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nc73EF ( NM_168703.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730225.1 Drosophila melanogaster neural conserved at 73EF, transcript variant C (Nc73EF), mRNA.

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CloneID	OFa18774
Clone ID Related Accession (Same CDS sequence)	NM_176341.3 , NM_168701.5 , NM_168703.5 , NM_168702.2
Accession Version	NM_168703.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neural conserved at 73EF, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_168703.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATAGAG CCCACACAGC CTTCAGCCTG GCCTTGTCGC CGATGGCGCA CAAGAACTTC 
GCCACATGGC TGCTCAAGAG CAGCAGCAGT CAGCAGATGG CCAAGGTAAC TGCAGCGGCC 
GCTGTGCGCA CCTACAACTC CGCAGCTGCC GAACCTTTTG CCAACGGCAG CACCGCCTCC 
TACGTGGAGG AGATGTACAA CGCCTGGCTT CGGGATCCCA CTTCCGTGCA CACCTCCTGG 
GACGCGTACT TCCGCAGCAA CAGCTACGTT AGCCCGCCCA ACTTGGCGCC CGTGCAGGCC 
AACACTCTGC CGCTGACCGC CTTCAACTTT GGAGGAGCCG TCGCCGGCGC TGCGCCCGAC 
TCCAAGACCA TTGACGACCA TCTGGCCGTC CAGGCCATCA TCAGGAGCTA CCAGATTCGA 
GGACATAATA TTGCTCACCT CGATCCACTT GAAATTAATA CACCAGAATT GCCTGGCAAC 
TCCTCGACGA AATCCATTTA CGCTAATTTC AGTTTTGGCG AGCAGGACAT GGATCGCCAG 
TTCAAGCTGC CCAGCACTAC CTTCATCGGA GGCGATGAGG CTTCGTTGCC CCTAAAGGAA 
ATCCTGAACC GCCTGGAGAA TGTCTACTGT AATAAGATTG GCGTGGAGTT CATGTTCATC 
AACTCTCTGG AGCAATGCAA CTGGATCCGC AAGCGTTTTG AGACCCCCGG CGTCCTTAAC 
TTCTCGCCCG AGGAGAAGCG TCTGATCCTG GCCCGTTTGA CCCGTGCCAC CGGCTTTGAG 
GCTTTCCTGG CCAAGAAGTA CTCTTCTGAG AAACGTTTCG GTCTGGAGGG TTGCGAGATC 
ATGATCCCAG CCCTGAAGGA GATCATCGAC GTATCAACGG AGTTGGGAGT GGAGTCGGTC 
ATCATGGGCA TGCCCCATCG TGGACGTCTT AACACCTTGG CCAATGTATG CCGCAAGCCC 
TTGAACCAGA TCTTCACCCA GTTCGCTGGA CTGGAGGCTG CTGATGATGG CTCTGGTGAT 
GTCAAGTACC ATCTCGGTAC ATACATCGAG CGATTGAACC GCGTTACAAA CAAGAACATC 
CGCCTGGCTG TCGTGGCCAA CCCGTCCCAT CTGGAGGCTG TGGATCCCGT GGTGCAGGGC 
AAGACCCGTG CCGAGCAGTT CTACCGTGGC GACCAGGAGG GCAAGAAGGT TATGTCCATT 
TTGATCCACG GTGATGCCGC CTTCTGCGGA CAGGGCGTCG TCTATGAAAC TATGCATCTT 
TCGGACCTGC CCGACTACAC CACCCACGGA ACTATCCATG TGGTGGCCAA CAACCAGATC 
GGTTTCACCA CCGATCCCCG TTTCTCGAGG TCCTCTCCCT ACTGTACGGA TGTGGCTCGT 
GTCGTCAATG CTCCTATTTT CCACGTCAAC GCTGACGATC CAGAGGCCGT GATGCATGTG 
TGCAAGGTGG CTGCTGAGTG GCGTGCTACT TTCCACAAGG ATTGTGTTAT TGATTTGGTC 
GGATACCGCC GCAATGGACA CAACGAGATC GACGAGCCCA TGTTCACGCA ACCCTTGATG 
TACCAGAAGA TCCGCAAGCA CAAGAACTGT CTCGACCTGT ATGCCGACAA ACTGATCGCC 
GAGGGCACTG TCACCGCCGA GGAGGTTAAG TCGGTTGCCG CTAAATACGA GAACATTTGC 
GAGGAGGCAT TCGCTCTGGC CAAGACCGAG ACCCACGTCA AGTACAAGGA CTGGCTCGAC 
TCGCCCTGGT CCGGCTTTTT CGAGGGCAAA GACCCATTGA AGGTAGCGCC CACTGGAGTG 
AAGGAGGAGA CCCTCATCCA CATCGGCAAC CGCTTCTCGT CGCCACCACC GAACGCTGCT 
GAATTCGTGA TTCACAAGGG TCTATTGCGA GTGTTGGCCG CTCGCAAGGC AATGGTGGAC 
GAAAAGGTCG CTGATTGGGC TTTGGGAGAG GCCATGGCCT TCGGCTCTCT GCTGAAGGAG 
GGAATCCATG TGCGCCTTTC GGGACAGGAT GTCGAGCGTG GTACCTTCTC GCATCGCCAC 
CATGTGCTGC ACCACCAGCT GGTCGACAAG GCCACTTACA ACTCGCTGCA GCACATGTAC 
CCAGATCAGG CGCCCTACTC CGTCTCCAAC AGCTCGCTGT CGGAGTACGC AGTGCTTGGT 
TTCGAGCACG GTTACTCGAT GACCAATCCC AATGCCCTGG TGCTGTGGGA GGCTCAGTTC 
GGAGACTTCA GCAACACGGC CCAGTCGATC ATCGATCAGT TCATCTCCAG CGGTCAGTCA 
AAGTGGGTGC GCCAATCGGG TCTGGTAATG CTCCTGCCCC ACGGCATGGA AGGCATGGGC 
CCCGAGCACT CCTCGTGCCG CGTGGAGCGC TTCCTGCAAA TGAGCAGTGA CGATCCCGAC 
TACTTCCCAC CAGAGTCCGA TGAGTTCGGA GTGCGACAGC TGCACGACAT TAACTGGATC 
GTGGCAAACT GCTCGACGCC CGCCAACTAC TACCACATCC TGCGCCGACA GATCGCATTG 
CCTTTCCGCA AGCCCCTGAT TCTGTGCACG CCCAAATCGC TGCTGCGTCA CCCCGAGGCT 
AAGAGTCCTT TCAGCGAGAT GAGCGAGGGC AGCGAGTTCC AGCGCATCAT CCCCGACAAC 
GGTCCCGCCG GCCAGAATCC CAGCAATGTG AAGAAGGTCG TCTTCTGCTC GGGCCGCGTC 
TACTACGATT TGACCAAGAC ACGTAGGGAA AAGCAGCTGG AGGGCGAAAT CGCCATTGTG 
CGCGTGGAGC AGATCTCTCC CTTCCCCTTC GATCTGGTCA AGGAGCAGGC CAACCTGTAC 
AAGAATGCCG AACTCGTGTG GGCCCAAGAG GAGCACAAGA ACCAGGGCAG CTGGACCTAT 
GTGCAACCTC GTTTCCTCAC CGCCTTGAAC CACAGCCGCG ACGTCAGCTA CGTCGGACGC 
GCCTGCGGAG CCTCCACCGC CACCGGATCC AAGGCCCAGC ACATCCGTGA GCTGAATGCG 
CTGCTCAACG ACGCGATTTC GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730225.1
CDS	115..3141
Translation	MHRAHTAFSLALSPMAHKNFATWLLKSSSSQQMAKVTAAAAVRTYNSAAAEPFANGSTASYVEEMYNAWLRDPTSVHTSWDAYFRSNSYVSPPNLAPVQANTLPLTAFNFGGAVAGAAPDSKTIDDHLAVQAIIRSYQIRGHNIAHLDPLEINTPELPGNSSTKSIYANFSFGEQDMDRQFKLPSTTFIGGDEASLPLKEILNRLENVYCNKIGVEFMFINSLEQCNWIRKRFETPGVLNFSPEEKRLILARLTRATGFEAFLAKKYSSEKRFGLEGCEIMIPALKEIIDVSTELGVESVIMGMPHRGRLNTLANVCRKPLNQIFTQFAGLEAADDGSGDVKYHLGTYIERLNRVTNKNIRLAVVANPSHLEAVDPVVQGKTRAEQFYRGDQEGKKVMSILIHGDAAFCGQGVVYETMHLSDLPDYTTHGTIHVVANNQIGFTTDPRFSRSSPYCTDVARVVNAPIFHVNADDPEAVMHVCKVAAEWRATFHKDCVIDLVGYRRNGHNEIDEPMFTQPLMYQKIRKHKNCLDLYADKLIAEGTVTAEEVKSVAAKYENICEEAFALAKTETHVKYKDWLDSPWSGFFEGKDPLKVAPTGVKEETLIHIGNRFSSPPPNAAEFVIHKGLLRVLAARKAMVDEKVADWALGEAMAFGSLLKEGIHVRLSGQDVERGTFSHRHHVLHHQLVDKATYNSLQHMYPDQAPYSVSNSSLSEYAVLGFEHGYSMTNPNALVLWEAQFGDFSNTAQSIIDQFISSGQSKWVRQSGLVMLLPHGMEGMGPEHSSCRVERFLQMSSDDPDYFPPESDEFGVRQLHDINWIVANCSTPANYYHILRRQIALPFRKPLILCTPKSLLRHPEAKSPFSEMSEGSEFQRIIPDNGPAGQNPSNVKKVVFCSGRVYYDLTKTRREKQLEGEIAIVRVEQISPFPFDLVKEQANLYKNAELVWAQEEHKNQGSWTYVQPRFLTALNHSRDVSYVGRACGASTATGSKAQHIRELNALLNDAIST

Target ORF information:

RefSeq Version	NM_168703.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neural conserved at 73EF, transcript variant C (Nc73EF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168703.5

ORF Insert Sequence:

ATGCATAGAG CCCACACAGC CTTCAGCCTG GCCTTGTCGC CGATGGCGCA CAAGAACTTC 
GCCACATGGC TGCTCAAGAG CAGCAGCAGT CAGCAGATGG CCAAGGTAAC TGCAGCGGCC 
GCTGTGCGCA CCTACAACTC CGCAGCTGCC GAACCTTTTG CCAACGGCAG CACCGCCTCC 
TACGTGGAGG AGATGTACAA CGCCTGGCTT CGGGATCCCA CTTCCGTGCA CACCTCCTGG 
GACGCGTACT TCCGCAGCAA CAGCTACGTT AGCCCGCCCA ACTTGGCGCC CGTGCAGGCC 
AACACTCTGC CGCTGACCGC CTTCAACTTT GGAGGAGCCG TCGCCGGCGC TGCGCCCGAC 
TCCAAGACCA TTGACGACCA TCTGGCCGTC CAGGCCATCA TCAGGAGCTA CCAGATTCGA 
GGACATAATA TTGCTCACCT CGATCCACTT GAAATTAATA CACCAGAATT GCCTGGCAAC 
TCCTCGACGA AATCCATTTA CGCTAATTTC AGTTTTGGCG AGCAGGACAT GGATCGCCAG 
TTCAAGCTGC CCAGCACTAC CTTCATCGGA GGCGATGAGG CTTCGTTGCC CCTAAAGGAA 
ATCCTGAACC GCCTGGAGAA TGTCTACTGT AATAAGATTG GCGTGGAGTT CATGTTCATC 
AACTCTCTGG AGCAATGCAA CTGGATCCGC AAGCGTTTTG AGACCCCCGG CGTCCTTAAC 
TTCTCGCCCG AGGAGAAGCG TCTGATCCTG GCCCGTTTGA CCCGTGCCAC CGGCTTTGAG 
GCTTTCCTGG CCAAGAAGTA CTCTTCTGAG AAACGTTTCG GTCTGGAGGG TTGCGAGATC 
ATGATCCCAG CCCTGAAGGA GATCATCGAC GTATCAACGG AGTTGGGAGT GGAGTCGGTC 
ATCATGGGCA TGCCCCATCG TGGACGTCTT AACACCTTGG CCAATGTATG CCGCAAGCCC 
TTGAACCAGA TCTTCACCCA GTTCGCTGGA CTGGAGGCTG CTGATGATGG CTCTGGTGAT 
GTCAAGTACC ATCTCGGTAC ATACATCGAG CGATTGAACC GCGTTACAAA CAAGAACATC 
CGCCTGGCTG TCGTGGCCAA CCCGTCCCAT CTGGAGGCTG TGGATCCCGT GGTGCAGGGC 
AAGACCCGTG CCGAGCAGTT CTACCGTGGC GACCAGGAGG GCAAGAAGGT TATGTCCATT 
TTGATCCACG GTGATGCCGC CTTCTGCGGA CAGGGCGTCG TCTATGAAAC TATGCATCTT 
TCGGACCTGC CCGACTACAC CACCCACGGA ACTATCCATG TGGTGGCCAA CAACCAGATC 
GGTTTCACCA CCGATCCCCG TTTCTCGAGG TCCTCTCCCT ACTGTACGGA TGTGGCTCGT 
GTCGTCAATG CTCCTATTTT CCACGTCAAC GCTGACGATC CAGAGGCCGT GATGCATGTG 
TGCAAGGTGG CTGCTGAGTG GCGTGCTACT TTCCACAAGG ATTGTGTTAT TGATTTGGTC 
GGATACCGCC GCAATGGACA CAACGAGATC GACGAGCCCA TGTTCACGCA ACCCTTGATG 
TACCAGAAGA TCCGCAAGCA CAAGAACTGT CTCGACCTGT ATGCCGACAA ACTGATCGCC 
GAGGGCACTG TCACCGCCGA GGAGGTTAAG TCGGTTGCCG CTAAATACGA GAACATTTGC 
GAGGAGGCAT TCGCTCTGGC CAAGACCGAG ACCCACGTCA AGTACAAGGA CTGGCTCGAC 
TCGCCCTGGT CCGGCTTTTT CGAGGGCAAA GACCCATTGA AGGTAGCGCC CACTGGAGTG 
AAGGAGGAGA CCCTCATCCA CATCGGCAAC CGCTTCTCGT CGCCACCACC GAACGCTGCT 
GAATTCGTGA TTCACAAGGG TCTATTGCGA GTGTTGGCCG CTCGCAAGGC AATGGTGGAC 
GAAAAGGTCG CTGATTGGGC TTTGGGAGAG GCCATGGCCT TCGGCTCTCT GCTGAAGGAG 
GGAATCCATG TGCGCCTTTC GGGACAGGAT GTCGAGCGTG GTACCTTCTC GCATCGCCAC 
CATGTGCTGC ACCACCAGCT GGTCGACAAG GCCACTTACA ACTCGCTGCA GCACATGTAC 
CCAGATCAGG CGCCCTACTC CGTCTCCAAC AGCTCGCTGT CGGAGTACGC AGTGCTTGGT 
TTCGAGCACG GTTACTCGAT GACCAATCCC AATGCCCTGG TGCTGTGGGA GGCTCAGTTC 
GGAGACTTCA GCAACACGGC CCAGTCGATC ATCGATCAGT TCATCTCCAG CGGTCAGTCA 
AAGTGGGTGC GCCAATCGGG TCTGGTAATG CTCCTGCCCC ACGGCATGGA AGGCATGGGC 
CCCGAGCACT CCTCGTGCCG CGTGGAGCGC TTCCTGCAAA TGAGCAGTGA CGATCCCGAC 
TACTTCCCAC CAGAGTCCGA TGAGTTCGGA GTGCGACAGC TGCACGACAT TAACTGGATC 
GTGGCAAACT GCTCGACGCC CGCCAACTAC TACCACATCC TGCGCCGACA GATCGCATTG 
CCTTTCCGCA AGCCCCTGAT TCTGTGCACG CCCAAATCGC TGCTGCGTCA CCCCGAGGCT 
AAGAGTCCTT TCAGCGAGAT GAGCGAGGGC AGCGAGTTCC AGCGCATCAT CCCCGACAAC 
GGTCCCGCCG GCCAGAATCC CAGCAATGTG AAGAAGGTCG TCTTCTGCTC GGGCCGCGTC 
TACTACGATT TGACCAAGAC ACGTAGGGAA AAGCAGCTGG AGGGCGAAAT CGCCATTGTG 
CGCGTGGAGC AGATCTCTCC CTTCCCCTTC GATCTGGTCA AGGAGCAGGC CAACCTGTAC 
AAGAATGCCG AACTCGTGTG GGCCCAAGAG GAGCACAAGA ACCAGGGCAG CTGGACCTAT 
GTGCAACCTC GTTTCCTCAC CGCCTTGAAC CACAGCCGCG ACGTCAGCTA CGTCGGACGC 
GCCTGCGGAG CCTCCACCGC CACCGGATCC AAGGCCCAGC ACATCCGTGA GCTGAATGCG 
CTGCTCAACG ACGCGATTTC GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nc73EF ( NM_168702.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730224.1 Drosophila melanogaster neural conserved at 73EF, transcript variant B (Nc73EF), mRNA.

Price & Availability↑

CloneID	OFa18774
Clone ID Related Accession (Same CDS sequence)	NM_176341.3 , NM_168701.5 , NM_168703.5 , NM_168702.2
Accession Version	NM_168702.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neural conserved at 73EF, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Dec 17, 2009 this sequence version replaced NM_168702.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATAGAG CCCACACAGC CTTCAGCCTG GCCTTGTCGC CGATGGCGCA CAAGAACTTC 
GCCACATGGC TGCTCAAGAG CAGCAGCAGT CAGCAGATGG CCAAGGTAAC TGCAGCGGCC 
GCTGTGCGCA CCTACAACTC CGCAGCTGCC GAACCTTTTG CCAACGGCAG CACCGCCTCC 
TACGTGGAGG AGATGTACAA CGCCTGGCTT CGGGATCCCA CTTCCGTGCA CACCTCCTGG 
GACGCGTACT TCCGCAGCAA CAGCTACGTT AGCCCGCCCA ACTTGGCGCC CGTGCAGGCC 
AACACTCTGC CGCTGACCGC CTTCAACTTT GGAGGAGCCG TCGCCGGCGC TGCGCCCGAC 
TCCAAGACCA TTGACGACCA TCTGGCCGTC CAGGCCATCA TCAGGAGCTA CCAGATTCGA 
GGACATAATA TTGCTCACCT CGATCCACTT GAAATTAATA CACCAGAATT GCCTGGCAAC 
TCCTCGACGA AATCCATTTA CGCTAATTTC AGTTTTGGCG AGCAGGACAT GGATCGCCAG 
TTCAAGCTGC CCAGCACTAC CTTCATCGGA GGCGATGAGG CTTCGTTGCC CCTAAAGGAA 
ATCCTGAACC GCCTGGAGAA TGTCTACTGT AATAAGATTG GCGTGGAGTT CATGTTCATC 
AACTCTCTGG AGCAATGCAA CTGGATCCGC AAGCGTTTTG AGACCCCCGG CGTCCTTAAC 
TTCTCGCCCG AGGAGAAGCG TCTGATCCTG GCCCGTTTGA CCCGTGCCAC CGGCTTTGAG 
GCTTTCCTGG CCAAGAAGTA CTCTTCTGAG AAACGTTTCG GTCTGGAGGG TTGCGAGATC 
ATGATCCCAG CCCTGAAGGA GATCATCGAC GTATCAACGG AGTTGGGAGT GGAGTCGGTC 
ATCATGGGCA TGCCCCATCG TGGACGTCTT AACACCTTGG CCAATGTATG CCGCAAGCCC 
TTGAACCAGA TCTTCACCCA GTTCGCTGGA CTGGAGGCTG CTGATGATGG CTCTGGTGAT 
GTCAAGTACC ATCTCGGTAC ATACATCGAG CGATTGAACC GCGTTACAAA CAAGAACATC 
CGCCTGGCTG TCGTGGCCAA CCCGTCCCAT CTGGAGGCTG TGGATCCCGT GGTGCAGGGC 
AAGACCCGTG CCGAGCAGTT CTACCGTGGC GACCAGGAGG GCAAGAAGGT TATGTCCATT 
TTGATCCACG GTGATGCCGC CTTCTGCGGA CAGGGCGTCG TCTATGAAAC TATGCATCTT 
TCGGACCTGC CCGACTACAC CACCCACGGA ACTATCCATG TGGTGGCCAA CAACCAGATC 
GGTTTCACCA CCGATCCCCG TTTCTCGAGG TCCTCTCCCT ACTGTACGGA TGTGGCTCGT 
GTCGTCAATG CTCCTATTTT CCACGTCAAC GCTGACGATC CAGAGGCCGT GATGCATGTG 
TGCAAGGTGG CTGCTGAGTG GCGTGCTACT TTCCACAAGG ATTGTGTTAT TGATTTGGTC 
GGATACCGCC GCAATGGACA CAACGAGATC GACGAGCCCA TGTTCACGCA ACCCTTGATG 
TACCAGAAGA TCCGCAAGCA CAAGAACTGT CTCGACCTGT ATGCCGACAA ACTGATCGCC 
GAGGGCACTG TCACCGCCGA GGAGGTTAAG TCGGTTGCCG CTAAATACGA GAACATTTGC 
GAGGAGGCAT TCGCTCTGGC CAAGACCGAG ACCCACGTCA AGTACAAGGA CTGGCTCGAC 
TCGCCCTGGT CCGGCTTTTT CGAGGGCAAA GACCCATTGA AGGTAGCGCC CACTGGAGTG 
AAGGAGGAGA CCCTCATCCA CATCGGCAAC CGCTTCTCGT CGCCACCACC GAACGCTGCT 
GAATTCGTGA TTCACAAGGG TCTATTGCGA GTGTTGGCCG CTCGCAAGGC AATGGTGGAC 
GAAAAGGTCG CTGATTGGGC TTTGGGAGAG GCCATGGCCT TCGGCTCTCT GCTGAAGGAG 
GGAATCCATG TGCGCCTTTC GGGACAGGAT GTCGAGCGTG GTACCTTCTC GCATCGCCAC 
CATGTGCTGC ACCACCAGCT GGTCGACAAG GCCACTTACA ACTCGCTGCA GCACATGTAC 
CCAGATCAGG CGCCCTACTC CGTCTCCAAC AGCTCGCTGT CGGAGTACGC AGTGCTTGGT 
TTCGAGCACG GTTACTCGAT GACCAATCCC AATGCCCTGG TGCTGTGGGA GGCTCAGTTC 
GGAGACTTCA GCAACACGGC CCAGTCGATC ATCGATCAGT TCATCTCCAG CGGTCAGTCA 
AAGTGGGTGC GCCAATCGGG TCTGGTAATG CTCCTGCCCC ACGGCATGGA AGGCATGGGC 
CCCGAGCACT CCTCGTGCCG CGTGGAGCGC TTCCTGCAAA TGAGCAGTGA CGATCCCGAC 
TACTTCCCAC CAGAGTCCGA TGAGTTCGGA GTGCGACAGC TGCACGACAT TAACTGGATC 
GTGGCAAACT GCTCGACGCC CGCCAACTAC TACCACATCC TGCGCCGACA GATCGCATTG 
CCTTTCCGCA AGCCCCTGAT TCTGTGCACG CCCAAATCGC TGCTGCGTCA CCCCGAGGCT 
AAGAGTCCTT TCAGCGAGAT GAGCGAGGGC AGCGAGTTCC AGCGCATCAT CCCCGACAAC 
GGTCCCGCCG GCCAGAATCC CAGCAATGTG AAGAAGGTCG TCTTCTGCTC GGGCCGCGTC 
TACTACGATT TGACCAAGAC ACGTAGGGAA AAGCAGCTGG AGGGCGAAAT CGCCATTGTG 
CGCGTGGAGC AGATCTCTCC CTTCCCCTTC GATCTGGTCA AGGAGCAGGC CAACCTGTAC 
AAGAATGCCG AACTCGTGTG GGCCCAAGAG GAGCACAAGA ACCAGGGCAG CTGGACCTAT 
GTGCAACCTC GTTTCCTCAC CGCCTTGAAC CACAGCCGCG ACGTCAGCTA CGTCGGACGC 
GCCTGCGGAG CCTCCACCGC CACCGGATCC AAGGCCCAGC ACATCCGTGA GCTGAATGCG 
CTGCTCAACG ACGCGATTTC GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730224.1
CDS	73..3099
Translation	MHRAHTAFSLALSPMAHKNFATWLLKSSSSQQMAKVTAAAAVRTYNSAAAEPFANGSTASYVEEMYNAWLRDPTSVHTSWDAYFRSNSYVSPPNLAPVQANTLPLTAFNFGGAVAGAAPDSKTIDDHLAVQAIIRSYQIRGHNIAHLDPLEINTPELPGNSSTKSIYANFSFGEQDMDRQFKLPSTTFIGGDEASLPLKEILNRLENVYCNKIGVEFMFINSLEQCNWIRKRFETPGVLNFSPEEKRLILARLTRATGFEAFLAKKYSSEKRFGLEGCEIMIPALKEIIDVSTELGVESVIMGMPHRGRLNTLANVCRKPLNQIFTQFAGLEAADDGSGDVKYHLGTYIERLNRVTNKNIRLAVVANPSHLEAVDPVVQGKTRAEQFYRGDQEGKKVMSILIHGDAAFCGQGVVYETMHLSDLPDYTTHGTIHVVANNQIGFTTDPRFSRSSPYCTDVARVVNAPIFHVNADDPEAVMHVCKVAAEWRATFHKDCVIDLVGYRRNGHNEIDEPMFTQPLMYQKIRKHKNCLDLYADKLIAEGTVTAEEVKSVAAKYENICEEAFALAKTETHVKYKDWLDSPWSGFFEGKDPLKVAPTGVKEETLIHIGNRFSSPPPNAAEFVIHKGLLRVLAARKAMVDEKVADWALGEAMAFGSLLKEGIHVRLSGQDVERGTFSHRHHVLHHQLVDKATYNSLQHMYPDQAPYSVSNSSLSEYAVLGFEHGYSMTNPNALVLWEAQFGDFSNTAQSIIDQFISSGQSKWVRQSGLVMLLPHGMEGMGPEHSSCRVERFLQMSSDDPDYFPPESDEFGVRQLHDINWIVANCSTPANYYHILRRQIALPFRKPLILCTPKSLLRHPEAKSPFSEMSEGSEFQRIIPDNGPAGQNPSNVKKVVFCSGRVYYDLTKTRREKQLEGEIAIVRVEQISPFPFDLVKEQANLYKNAELVWAQEEHKNQGSWTYVQPRFLTALNHSRDVSYVGRACGASTATGSKAQHIRELNALLNDAIST

Target ORF information:

RefSeq Version	NM_168702.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neural conserved at 73EF, transcript variant B (Nc73EF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168702.2

ORF Insert Sequence:

ATGCATAGAG CCCACACAGC CTTCAGCCTG GCCTTGTCGC CGATGGCGCA CAAGAACTTC 
GCCACATGGC TGCTCAAGAG CAGCAGCAGT CAGCAGATGG CCAAGGTAAC TGCAGCGGCC 
GCTGTGCGCA CCTACAACTC CGCAGCTGCC GAACCTTTTG CCAACGGCAG CACCGCCTCC 
TACGTGGAGG AGATGTACAA CGCCTGGCTT CGGGATCCCA CTTCCGTGCA CACCTCCTGG 
GACGCGTACT TCCGCAGCAA CAGCTACGTT AGCCCGCCCA ACTTGGCGCC CGTGCAGGCC 
AACACTCTGC CGCTGACCGC CTTCAACTTT GGAGGAGCCG TCGCCGGCGC TGCGCCCGAC 
TCCAAGACCA TTGACGACCA TCTGGCCGTC CAGGCCATCA TCAGGAGCTA CCAGATTCGA 
GGACATAATA TTGCTCACCT CGATCCACTT GAAATTAATA CACCAGAATT GCCTGGCAAC 
TCCTCGACGA AATCCATTTA CGCTAATTTC AGTTTTGGCG AGCAGGACAT GGATCGCCAG 
TTCAAGCTGC CCAGCACTAC CTTCATCGGA GGCGATGAGG CTTCGTTGCC CCTAAAGGAA 
ATCCTGAACC GCCTGGAGAA TGTCTACTGT AATAAGATTG GCGTGGAGTT CATGTTCATC 
AACTCTCTGG AGCAATGCAA CTGGATCCGC AAGCGTTTTG AGACCCCCGG CGTCCTTAAC 
TTCTCGCCCG AGGAGAAGCG TCTGATCCTG GCCCGTTTGA CCCGTGCCAC CGGCTTTGAG 
GCTTTCCTGG CCAAGAAGTA CTCTTCTGAG AAACGTTTCG GTCTGGAGGG TTGCGAGATC 
ATGATCCCAG CCCTGAAGGA GATCATCGAC GTATCAACGG AGTTGGGAGT GGAGTCGGTC 
ATCATGGGCA TGCCCCATCG TGGACGTCTT AACACCTTGG CCAATGTATG CCGCAAGCCC 
TTGAACCAGA TCTTCACCCA GTTCGCTGGA CTGGAGGCTG CTGATGATGG CTCTGGTGAT 
GTCAAGTACC ATCTCGGTAC ATACATCGAG CGATTGAACC GCGTTACAAA CAAGAACATC 
CGCCTGGCTG TCGTGGCCAA CCCGTCCCAT CTGGAGGCTG TGGATCCCGT GGTGCAGGGC 
AAGACCCGTG CCGAGCAGTT CTACCGTGGC GACCAGGAGG GCAAGAAGGT TATGTCCATT 
TTGATCCACG GTGATGCCGC CTTCTGCGGA CAGGGCGTCG TCTATGAAAC TATGCATCTT 
TCGGACCTGC CCGACTACAC CACCCACGGA ACTATCCATG TGGTGGCCAA CAACCAGATC 
GGTTTCACCA CCGATCCCCG TTTCTCGAGG TCCTCTCCCT ACTGTACGGA TGTGGCTCGT 
GTCGTCAATG CTCCTATTTT CCACGTCAAC GCTGACGATC CAGAGGCCGT GATGCATGTG 
TGCAAGGTGG CTGCTGAGTG GCGTGCTACT TTCCACAAGG ATTGTGTTAT TGATTTGGTC 
GGATACCGCC GCAATGGACA CAACGAGATC GACGAGCCCA TGTTCACGCA ACCCTTGATG 
TACCAGAAGA TCCGCAAGCA CAAGAACTGT CTCGACCTGT ATGCCGACAA ACTGATCGCC 
GAGGGCACTG TCACCGCCGA GGAGGTTAAG TCGGTTGCCG CTAAATACGA GAACATTTGC 
GAGGAGGCAT TCGCTCTGGC CAAGACCGAG ACCCACGTCA AGTACAAGGA CTGGCTCGAC 
TCGCCCTGGT CCGGCTTTTT CGAGGGCAAA GACCCATTGA AGGTAGCGCC CACTGGAGTG 
AAGGAGGAGA CCCTCATCCA CATCGGCAAC CGCTTCTCGT CGCCACCACC GAACGCTGCT 
GAATTCGTGA TTCACAAGGG TCTATTGCGA GTGTTGGCCG CTCGCAAGGC AATGGTGGAC 
GAAAAGGTCG CTGATTGGGC TTTGGGAGAG GCCATGGCCT TCGGCTCTCT GCTGAAGGAG 
GGAATCCATG TGCGCCTTTC GGGACAGGAT GTCGAGCGTG GTACCTTCTC GCATCGCCAC 
CATGTGCTGC ACCACCAGCT GGTCGACAAG GCCACTTACA ACTCGCTGCA GCACATGTAC 
CCAGATCAGG CGCCCTACTC CGTCTCCAAC AGCTCGCTGT CGGAGTACGC AGTGCTTGGT 
TTCGAGCACG GTTACTCGAT GACCAATCCC AATGCCCTGG TGCTGTGGGA GGCTCAGTTC 
GGAGACTTCA GCAACACGGC CCAGTCGATC ATCGATCAGT TCATCTCCAG CGGTCAGTCA 
AAGTGGGTGC GCCAATCGGG TCTGGTAATG CTCCTGCCCC ACGGCATGGA AGGCATGGGC 
CCCGAGCACT CCTCGTGCCG CGTGGAGCGC TTCCTGCAAA TGAGCAGTGA CGATCCCGAC 
TACTTCCCAC CAGAGTCCGA TGAGTTCGGA GTGCGACAGC TGCACGACAT TAACTGGATC 
GTGGCAAACT GCTCGACGCC CGCCAACTAC TACCACATCC TGCGCCGACA GATCGCATTG 
CCTTTCCGCA AGCCCCTGAT TCTGTGCACG CCCAAATCGC TGCTGCGTCA CCCCGAGGCT 
AAGAGTCCTT TCAGCGAGAT GAGCGAGGGC AGCGAGTTCC AGCGCATCAT CCCCGACAAC 
GGTCCCGCCG GCCAGAATCC CAGCAATGTG AAGAAGGTCG TCTTCTGCTC GGGCCGCGTC 
TACTACGATT TGACCAAGAC ACGTAGGGAA AAGCAGCTGG AGGGCGAAAT CGCCATTGTG 
CGCGTGGAGC AGATCTCTCC CTTCCCCTTC GATCTGGTCA AGGAGCAGGC CAACCTGTAC 
AAGAATGCCG AACTCGTGTG GGCCCAAGAG GAGCACAAGA ACCAGGGCAG CTGGACCTAT 
GTGCAACCTC GTTTCCTCAC CGCCTTGAAC CACAGCCGCG ACGTCAGCTA CGTCGGACGC 
GCCTGCGGAG CCTCCACCGC CACCGGATCC AAGGCCCAGC ACATCCGTGA GCTGAATGCG 
CTGCTCAACG ACGCGATTTC GACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nc73EF ( NM_176342.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788520.2 Drosophila melanogaster neural conserved at 73EF, transcript variant H (Nc73EF), mRNA.

Price & Availability↑

CloneID	OFa18775
Clone ID Related Accession (Same CDS sequence)	NM_176342.5 , NM_176340.3
Accession Version	NM_176342.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neural conserved at 73EF, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_176342.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATAGAG CCCACACAGC CTTCAGCCTG GCCTTGTCGC CGATGGCGCA CAAGAACTTC 
GCCACATGGC TGCTCAAGAG CAGCAGCAGT CAGCAGATGG CCAAGGTAAC TGCAGCGGCC 
GCTGTGCGCA CCTACAACTC CGCAGCTGCC GAACCTTTTG CCAACGGCAG CACCGCCTCC 
TACGTGGAGG AGATGTACAA CGCCTGGCTT CGGGATCCCA CTTCCGTGCA CACCTCCTGG 
GACGCGTACT TCCGCAGCAA CAGCTACGTT AGCCCGCCCA ACTTGGCGCC CGTGCAGGCC 
AACACTCTGC CGCTGACCGC CTTCAACTTT GGAGGAGCCG TCGCCGGCGC TGCGCCCGAC 
TCCAAGACCA TTGACGACCA TCTGGCCGTC CAGGCCATCA TCAGGAGCTA CCAGTCACGA 
GGTCATTTGG CATCTGATCT GGATCCGCTT GGAATTTTGA CTCGCGAAAA AACCGTCTGC 
AAGGATGGGC TAGCACGTCG TGCTAACGAA GATGTGCTCA GGCAGCACTC TGGGTTTTTG 
TTTGGCGAGC AGGACATGGA TCGCCAGTTC AAGCTGCCCA GCACTACCTT CATCGGAGGC 
GATGAGGCTT CGTTGCCCCT AAAGGAAATC CTGAACCGCC TGGAGAATGT CTACTGTAAT 
AAGATTGGCG TGGAGTTCAT GTTCATCAAC TCTCTGGAGC AATGCAACTG GATCCGCAAG 
CGTTTTGAGA CCCCCGGCGT CCTTAACTTC TCGCCCGAGG AGAAGCGTCT GATCCTGGCC 
CGTTTGACCC GTGCCACCGG CTTTGAGGCT TTCCTGGCCA AGAAGTACTC TTCTGAGAAA 
CGTTTCGGTC TGGAGGGTTG CGAGATCATG ATCCCAGCCC TGAAGGAGAT CATCGACGTA 
TCAACGGAGT TGGGAGTGGA GTCGGTCATC ATGGGCATGC CCCATCGTGG ACGTCTTAAC 
ACCTTGGCCA ATGTATGCCG CAAGCCCTTG AACCAGATCT TCACCCAGTT CGCTGGACTG 
GAGGCTGCTG ATGATGGCTC TGGTGATGTC AAGTACCATC TCGGTACATA CATCGAGCGA 
TTGAACCGCG TTACAAACAA GAACATCCGC CTGGCTGTCG TGGCCAACCC GTCCCATCTG 
GAGGCTGTGG ATCCCGTGGT GCAGGGCAAG ACCCGTGCCG AGCAGTTCTA CCGTGGCGAC 
CAGGAGGGCA AGAAGGTTAT GTCCATTTTG ATCCACGGTG ATGCCGCCTT CTGCGGACAG 
GGCGTCGTCT ATGAAACTAT GCATCTTTCG GACCTGCCCG ACTACACCAC CCACGGAACT 
ATCCATGTGG TGGCCAACAA CCAGATCGGT TTCACCACCG ATCCCCGTTT CTCGAGGTCC 
TCTCCCTACT GTACGGATGT GGCTCGTGTC GTCAATGCTC CTATTTTCCA CGTCAACGCT 
GACGATCCAG AGGCCGTGAT GCATGTGTGC AAGGTGGCTG CTGAGTGGCG TGCTACTTTC 
CACAAGGATT GTGTTATTGA TTTGGTCGGA TACCGCCGCA ATGGACACAA CGAGATCGAC 
GAGCCCATGT TCACGCAACC CTTGATGTAC CAGAAGATCC GCAAGCACAA GAACTGTCTC 
GACCTGTATG CCGACAAACT GATCGCCGAG GGCACTGTCA CCGCCGAGGA GGTTAAGTCG 
GTTGCCGCTA AATACGAGAA CATTTGCGAG GAGGCATTCG CTCTGGCCAA GACCGAGACC 
CACGTCAAGT ACAAGGACTG GCTCGACTCG CCCTGGTCCG GCTTTTTCGA GGGCAAAGAC 
CCATTGAAGG TAGCGCCCAC TGGAGTGAAG GAGGAGACCC TCATCCACAT CGGCAACCGC 
TTCTCGTCGC CACCACCGAA CGCTGCTGAA TTCGTGATTC ACAAGGGTCT ATTGCGAGTG 
TTGGCCGCTC GCAAGGCAAT GGTGGACGAA AAGGTCGCTG ATTGGGCTTT GGGAGAGGCC 
ATGGCCTTCG GCTCTCTGCT GAAGGAGGGA ATCCATGTGC GCCTTTCGGG ACAGGATGTC 
GAGCGTGGTA CCTTCTCGCA TCGCCACCAT GTGCTGCACC ACCAGCTGGT CGACAAGGCC 
ACTTACAACT CGCTGCAGCA CATGTACCCA GATCAGGCGC CCTACTCCGT CTCCAACAGC 
TCGCTGTCGG AGTACGCAGT GCTTGGTTTC GAGCACGGTT ACTCGATGAC CAATCCCAAT 
GCCCTGGTGC TGTGGGAGGC TCAGTTCGGA GACTTCAGCA ACACGGCCCA GTCGATCATC 
GATCAGTTCA TCTCCAGCGG TCAGTCAAAG TGGGTGCGCC AATCGGGTCT GGTAATGCTC 
CTGCCCCACG GCATGGAAGG CATGGGCCCC GAGCACTCCT CGTGCCGCGT GGAGCGCTTC 
CTGCAAATGA GCAGTGACGA TCCCGACTAC TTCCCACCAG AGTCCGATGA GTTCGGAGTG 
CGACAGCTGC ACGACATTAA CTGGATCGTG GCAAACTGCT CGACGCCCGC CAACTACTAC 
CACATCCTGC GCCGACAGAT CGCATTGCCT TTCCGCAAGC CCCTGATTCT GTGCACGCCC 
AAATCGCTGC TGCGTCACCC CGAGGCTAAG AGTCCTTTCA GCGAGATGAG CGAGGGCAGC 
GAGTTCCAGC GCATCATCCC CGACAACGGT CCCGCCGGCC AGAATCCCAG CAATGTGAAG 
AAGGTCGTCT TCTGCTCGGG CCGCGTCTAC TACGATTTGA CCAAGACACG TAGGGAAAAG 
CAGCTGGAGG GCGAAATCGC CATTGTGCGC GTGGAGCAGA TCTCTCCCTT CCCCTTCGAT 
CTGGTCAAGG AGCAGGCCAA CCTGTACAAG AATGCCGAAC TCGTGTGGGC CCAAGAGGAG 
CACAAGAACC AGGGCAGCTG GACCTATGTG CAACCTCGTT TCCTCACCGC CTTGAACCAC 
AGCCGCGACG TCAGCTACGT CGGACGCGCC TGCGGAGCCT CCACCGCCAC CGGATCCAAG 
GCCCAGCACA TCCGTGAGCT GAATGCGCTG CTCAACGACG CGATTTCGAC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788520.2
CDS	90..3143
Translation	MHRAHTAFSLALSPMAHKNFATWLLKSSSSQQMAKVTAAAAVRTYNSAAAEPFANGSTASYVEEMYNAWLRDPTSVHTSWDAYFRSNSYVSPPNLAPVQANTLPLTAFNFGGAVAGAAPDSKTIDDHLAVQAIIRSYQSRGHLASDLDPLGILTREKTVCKDGLARRANEDVLRQHSGFLFGEQDMDRQFKLPSTTFIGGDEASLPLKEILNRLENVYCNKIGVEFMFINSLEQCNWIRKRFETPGVLNFSPEEKRLILARLTRATGFEAFLAKKYSSEKRFGLEGCEIMIPALKEIIDVSTELGVESVIMGMPHRGRLNTLANVCRKPLNQIFTQFAGLEAADDGSGDVKYHLGTYIERLNRVTNKNIRLAVVANPSHLEAVDPVVQGKTRAEQFYRGDQEGKKVMSILIHGDAAFCGQGVVYETMHLSDLPDYTTHGTIHVVANNQIGFTTDPRFSRSSPYCTDVARVVNAPIFHVNADDPEAVMHVCKVAAEWRATFHKDCVIDLVGYRRNGHNEIDEPMFTQPLMYQKIRKHKNCLDLYADKLIAEGTVTAEEVKSVAAKYENICEEAFALAKTETHVKYKDWLDSPWSGFFEGKDPLKVAPTGVKEETLIHIGNRFSSPPPNAAEFVIHKGLLRVLAARKAMVDEKVADWALGEAMAFGSLLKEGIHVRLSGQDVERGTFSHRHHVLHHQLVDKATYNSLQHMYPDQAPYSVSNSSLSEYAVLGFEHGYSMTNPNALVLWEAQFGDFSNTAQSIIDQFISSGQSKWVRQSGLVMLLPHGMEGMGPEHSSCRVERFLQMSSDDPDYFPPESDEFGVRQLHDINWIVANCSTPANYYHILRRQIALPFRKPLILCTPKSLLRHPEAKSPFSEMSEGSEFQRIIPDNGPAGQNPSNVKKVVFCSGRVYYDLTKTRREKQLEGEIAIVRVEQISPFPFDLVKEQANLYKNAELVWAQEEHKNQGSWTYVQPRFLTALNHSRDVSYVGRACGASTATGSKAQHIRELNALLNDAIST

Target ORF information:

RefSeq Version	NM_176342.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neural conserved at 73EF, transcript variant H (Nc73EF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176342.5

ORF Insert Sequence:

ATGCATAGAG CCCACACAGC CTTCAGCCTG GCCTTGTCGC CGATGGCGCA CAAGAACTTC 
GCCACATGGC TGCTCAAGAG CAGCAGCAGT CAGCAGATGG CCAAGGTAAC TGCAGCGGCC 
GCTGTGCGCA CCTACAACTC CGCAGCTGCC GAACCTTTTG CCAACGGCAG CACCGCCTCC 
TACGTGGAGG AGATGTACAA CGCCTGGCTT CGGGATCCCA CTTCCGTGCA CACCTCCTGG 
GACGCGTACT TCCGCAGCAA CAGCTACGTT AGCCCGCCCA ACTTGGCGCC CGTGCAGGCC 
AACACTCTGC CGCTGACCGC CTTCAACTTT GGAGGAGCCG TCGCCGGCGC TGCGCCCGAC 
TCCAAGACCA TTGACGACCA TCTGGCCGTC CAGGCCATCA TCAGGAGCTA CCAGTCACGA 
GGTCATTTGG CATCTGATCT GGATCCGCTT GGAATTTTGA CTCGCGAAAA AACCGTCTGC 
AAGGATGGGC TAGCACGTCG TGCTAACGAA GATGTGCTCA GGCAGCACTC TGGGTTTTTG 
TTTGGCGAGC AGGACATGGA TCGCCAGTTC AAGCTGCCCA GCACTACCTT CATCGGAGGC 
GATGAGGCTT CGTTGCCCCT AAAGGAAATC CTGAACCGCC TGGAGAATGT CTACTGTAAT 
AAGATTGGCG TGGAGTTCAT GTTCATCAAC TCTCTGGAGC AATGCAACTG GATCCGCAAG 
CGTTTTGAGA CCCCCGGCGT CCTTAACTTC TCGCCCGAGG AGAAGCGTCT GATCCTGGCC 
CGTTTGACCC GTGCCACCGG CTTTGAGGCT TTCCTGGCCA AGAAGTACTC TTCTGAGAAA 
CGTTTCGGTC TGGAGGGTTG CGAGATCATG ATCCCAGCCC TGAAGGAGAT CATCGACGTA 
TCAACGGAGT TGGGAGTGGA GTCGGTCATC ATGGGCATGC CCCATCGTGG ACGTCTTAAC 
ACCTTGGCCA ATGTATGCCG CAAGCCCTTG AACCAGATCT TCACCCAGTT CGCTGGACTG 
GAGGCTGCTG ATGATGGCTC TGGTGATGTC AAGTACCATC TCGGTACATA CATCGAGCGA 
TTGAACCGCG TTACAAACAA GAACATCCGC CTGGCTGTCG TGGCCAACCC GTCCCATCTG 
GAGGCTGTGG ATCCCGTGGT GCAGGGCAAG ACCCGTGCCG AGCAGTTCTA CCGTGGCGAC 
CAGGAGGGCA AGAAGGTTAT GTCCATTTTG ATCCACGGTG ATGCCGCCTT CTGCGGACAG 
GGCGTCGTCT ATGAAACTAT GCATCTTTCG GACCTGCCCG ACTACACCAC CCACGGAACT 
ATCCATGTGG TGGCCAACAA CCAGATCGGT TTCACCACCG ATCCCCGTTT CTCGAGGTCC 
TCTCCCTACT GTACGGATGT GGCTCGTGTC GTCAATGCTC CTATTTTCCA CGTCAACGCT 
GACGATCCAG AGGCCGTGAT GCATGTGTGC AAGGTGGCTG CTGAGTGGCG TGCTACTTTC 
CACAAGGATT GTGTTATTGA TTTGGTCGGA TACCGCCGCA ATGGACACAA CGAGATCGAC 
GAGCCCATGT TCACGCAACC CTTGATGTAC CAGAAGATCC GCAAGCACAA GAACTGTCTC 
GACCTGTATG CCGACAAACT GATCGCCGAG GGCACTGTCA CCGCCGAGGA GGTTAAGTCG 
GTTGCCGCTA AATACGAGAA CATTTGCGAG GAGGCATTCG CTCTGGCCAA GACCGAGACC 
CACGTCAAGT ACAAGGACTG GCTCGACTCG CCCTGGTCCG GCTTTTTCGA GGGCAAAGAC 
CCATTGAAGG TAGCGCCCAC TGGAGTGAAG GAGGAGACCC TCATCCACAT CGGCAACCGC 
TTCTCGTCGC CACCACCGAA CGCTGCTGAA TTCGTGATTC ACAAGGGTCT ATTGCGAGTG 
TTGGCCGCTC GCAAGGCAAT GGTGGACGAA AAGGTCGCTG ATTGGGCTTT GGGAGAGGCC 
ATGGCCTTCG GCTCTCTGCT GAAGGAGGGA ATCCATGTGC GCCTTTCGGG ACAGGATGTC 
GAGCGTGGTA CCTTCTCGCA TCGCCACCAT GTGCTGCACC ACCAGCTGGT CGACAAGGCC 
ACTTACAACT CGCTGCAGCA CATGTACCCA GATCAGGCGC CCTACTCCGT CTCCAACAGC 
TCGCTGTCGG AGTACGCAGT GCTTGGTTTC GAGCACGGTT ACTCGATGAC CAATCCCAAT 
GCCCTGGTGC TGTGGGAGGC TCAGTTCGGA GACTTCAGCA ACACGGCCCA GTCGATCATC 
GATCAGTTCA TCTCCAGCGG TCAGTCAAAG TGGGTGCGCC AATCGGGTCT GGTAATGCTC 
CTGCCCCACG GCATGGAAGG CATGGGCCCC GAGCACTCCT CGTGCCGCGT GGAGCGCTTC 
CTGCAAATGA GCAGTGACGA TCCCGACTAC TTCCCACCAG AGTCCGATGA GTTCGGAGTG 
CGACAGCTGC ACGACATTAA CTGGATCGTG GCAAACTGCT CGACGCCCGC CAACTACTAC 
CACATCCTGC GCCGACAGAT CGCATTGCCT TTCCGCAAGC CCCTGATTCT GTGCACGCCC 
AAATCGCTGC TGCGTCACCC CGAGGCTAAG AGTCCTTTCA GCGAGATGAG CGAGGGCAGC 
GAGTTCCAGC GCATCATCCC CGACAACGGT CCCGCCGGCC AGAATCCCAG CAATGTGAAG 
AAGGTCGTCT TCTGCTCGGG CCGCGTCTAC TACGATTTGA CCAAGACACG TAGGGAAAAG 
CAGCTGGAGG GCGAAATCGC CATTGTGCGC GTGGAGCAGA TCTCTCCCTT CCCCTTCGAT 
CTGGTCAAGG AGCAGGCCAA CCTGTACAAG AATGCCGAAC TCGTGTGGGC CCAAGAGGAG 
CACAAGAACC AGGGCAGCTG GACCTATGTG CAACCTCGTT TCCTCACCGC CTTGAACCAC 
AGCCGCGACG TCAGCTACGT CGGACGCGCC TGCGGAGCCT CCACCGCCAC CGGATCCAAG 
GCCCAGCACA TCCGTGAGCT GAATGCGCTG CTCAACGACG CGATTTCGAC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nc73EF ( NM_176340.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788518.1 Drosophila melanogaster neural conserved at 73EF, transcript variant F (Nc73EF), mRNA.

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CloneID	OFa18775
Clone ID Related Accession (Same CDS sequence)	NM_176342.5 , NM_176340.3
Accession Version	NM_176340.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neural conserved at 73EF, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_176340.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATAGAG CCCACACAGC CTTCAGCCTG GCCTTGTCGC CGATGGCGCA CAAGAACTTC 
GCCACATGGC TGCTCAAGAG CAGCAGCAGT CAGCAGATGG CCAAGGTAAC TGCAGCGGCC 
GCTGTGCGCA CCTACAACTC CGCAGCTGCC GAACCTTTTG CCAACGGCAG CACCGCCTCC 
TACGTGGAGG AGATGTACAA CGCCTGGCTT CGGGATCCCA CTTCCGTGCA CACCTCCTGG 
GACGCGTACT TCCGCAGCAA CAGCTACGTT AGCCCGCCCA ACTTGGCGCC CGTGCAGGCC 
AACACTCTGC CGCTGACCGC CTTCAACTTT GGAGGAGCCG TCGCCGGCGC TGCGCCCGAC 
TCCAAGACCA TTGACGACCA TCTGGCCGTC CAGGCCATCA TCAGGAGCTA CCAGTCACGA 
GGTCATTTGG CATCTGATCT GGATCCGCTT GGAATTTTGA CTCGCGAAAA AACCGTCTGC 
AAGGATGGGC TAGCACGTCG TGCTAACGAA GATGTGCTCA GGCAGCACTC TGGGTTTTTG 
TTTGGCGAGC AGGACATGGA TCGCCAGTTC AAGCTGCCCA GCACTACCTT CATCGGAGGC 
GATGAGGCTT CGTTGCCCCT AAAGGAAATC CTGAACCGCC TGGAGAATGT CTACTGTAAT 
AAGATTGGCG TGGAGTTCAT GTTCATCAAC TCTCTGGAGC AATGCAACTG GATCCGCAAG 
CGTTTTGAGA CCCCCGGCGT CCTTAACTTC TCGCCCGAGG AGAAGCGTCT GATCCTGGCC 
CGTTTGACCC GTGCCACCGG CTTTGAGGCT TTCCTGGCCA AGAAGTACTC TTCTGAGAAA 
CGTTTCGGTC TGGAGGGTTG CGAGATCATG ATCCCAGCCC TGAAGGAGAT CATCGACGTA 
TCAACGGAGT TGGGAGTGGA GTCGGTCATC ATGGGCATGC CCCATCGTGG ACGTCTTAAC 
ACCTTGGCCA ATGTATGCCG CAAGCCCTTG AACCAGATCT TCACCCAGTT CGCTGGACTG 
GAGGCTGCTG ATGATGGCTC TGGTGATGTC AAGTACCATC TCGGTACATA CATCGAGCGA 
TTGAACCGCG TTACAAACAA GAACATCCGC CTGGCTGTCG TGGCCAACCC GTCCCATCTG 
GAGGCTGTGG ATCCCGTGGT GCAGGGCAAG ACCCGTGCCG AGCAGTTCTA CCGTGGCGAC 
CAGGAGGGCA AGAAGGTTAT GTCCATTTTG ATCCACGGTG ATGCCGCCTT CTGCGGACAG 
GGCGTCGTCT ATGAAACTAT GCATCTTTCG GACCTGCCCG ACTACACCAC CCACGGAACT 
ATCCATGTGG TGGCCAACAA CCAGATCGGT TTCACCACCG ATCCCCGTTT CTCGAGGTCC 
TCTCCCTACT GTACGGATGT GGCTCGTGTC GTCAATGCTC CTATTTTCCA CGTCAACGCT 
GACGATCCAG AGGCCGTGAT GCATGTGTGC AAGGTGGCTG CTGAGTGGCG TGCTACTTTC 
CACAAGGATT GTGTTATTGA TTTGGTCGGA TACCGCCGCA ATGGACACAA CGAGATCGAC 
GAGCCCATGT TCACGCAACC CTTGATGTAC CAGAAGATCC GCAAGCACAA GAACTGTCTC 
GACCTGTATG CCGACAAACT GATCGCCGAG GGCACTGTCA CCGCCGAGGA GGTTAAGTCG 
GTTGCCGCTA AATACGAGAA CATTTGCGAG GAGGCATTCG CTCTGGCCAA GACCGAGACC 
CACGTCAAGT ACAAGGACTG GCTCGACTCG CCCTGGTCCG GCTTTTTCGA GGGCAAAGAC 
CCATTGAAGG TAGCGCCCAC TGGAGTGAAG GAGGAGACCC TCATCCACAT CGGCAACCGC 
TTCTCGTCGC CACCACCGAA CGCTGCTGAA TTCGTGATTC ACAAGGGTCT ATTGCGAGTG 
TTGGCCGCTC GCAAGGCAAT GGTGGACGAA AAGGTCGCTG ATTGGGCTTT GGGAGAGGCC 
ATGGCCTTCG GCTCTCTGCT GAAGGAGGGA ATCCATGTGC GCCTTTCGGG ACAGGATGTC 
GAGCGTGGTA CCTTCTCGCA TCGCCACCAT GTGCTGCACC ACCAGCTGGT CGACAAGGCC 
ACTTACAACT CGCTGCAGCA CATGTACCCA GATCAGGCGC CCTACTCCGT CTCCAACAGC 
TCGCTGTCGG AGTACGCAGT GCTTGGTTTC GAGCACGGTT ACTCGATGAC CAATCCCAAT 
GCCCTGGTGC TGTGGGAGGC TCAGTTCGGA GACTTCAGCA ACACGGCCCA GTCGATCATC 
GATCAGTTCA TCTCCAGCGG TCAGTCAAAG TGGGTGCGCC AATCGGGTCT GGTAATGCTC 
CTGCCCCACG GCATGGAAGG CATGGGCCCC GAGCACTCCT CGTGCCGCGT GGAGCGCTTC 
CTGCAAATGA GCAGTGACGA TCCCGACTAC TTCCCACCAG AGTCCGATGA GTTCGGAGTG 
CGACAGCTGC ACGACATTAA CTGGATCGTG GCAAACTGCT CGACGCCCGC CAACTACTAC 
CACATCCTGC GCCGACAGAT CGCATTGCCT TTCCGCAAGC CCCTGATTCT GTGCACGCCC 
AAATCGCTGC TGCGTCACCC CGAGGCTAAG AGTCCTTTCA GCGAGATGAG CGAGGGCAGC 
GAGTTCCAGC GCATCATCCC CGACAACGGT CCCGCCGGCC AGAATCCCAG CAATGTGAAG 
AAGGTCGTCT TCTGCTCGGG CCGCGTCTAC TACGATTTGA CCAAGACACG TAGGGAAAAG 
CAGCTGGAGG GCGAAATCGC CATTGTGCGC GTGGAGCAGA TCTCTCCCTT CCCCTTCGAT 
CTGGTCAAGG AGCAGGCCAA CCTGTACAAG AATGCCGAAC TCGTGTGGGC CCAAGAGGAG 
CACAAGAACC AGGGCAGCTG GACCTATGTG CAACCTCGTT TCCTCACCGC CTTGAACCAC 
AGCCGCGACG TCAGCTACGT CGGACGCGCC TGCGGAGCCT CCACCGCCAC CGGATCCAAG 
GCCCAGCACA TCCGTGAGCT GAATGCGCTG CTCAACGACG CGATTTCGAC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788518.1
CDS	66..3119
Translation	MHRAHTAFSLALSPMAHKNFATWLLKSSSSQQMAKVTAAAAVRTYNSAAAEPFANGSTASYVEEMYNAWLRDPTSVHTSWDAYFRSNSYVSPPNLAPVQANTLPLTAFNFGGAVAGAAPDSKTIDDHLAVQAIIRSYQSRGHLASDLDPLGILTREKTVCKDGLARRANEDVLRQHSGFLFGEQDMDRQFKLPSTTFIGGDEASLPLKEILNRLENVYCNKIGVEFMFINSLEQCNWIRKRFETPGVLNFSPEEKRLILARLTRATGFEAFLAKKYSSEKRFGLEGCEIMIPALKEIIDVSTELGVESVIMGMPHRGRLNTLANVCRKPLNQIFTQFAGLEAADDGSGDVKYHLGTYIERLNRVTNKNIRLAVVANPSHLEAVDPVVQGKTRAEQFYRGDQEGKKVMSILIHGDAAFCGQGVVYETMHLSDLPDYTTHGTIHVVANNQIGFTTDPRFSRSSPYCTDVARVVNAPIFHVNADDPEAVMHVCKVAAEWRATFHKDCVIDLVGYRRNGHNEIDEPMFTQPLMYQKIRKHKNCLDLYADKLIAEGTVTAEEVKSVAAKYENICEEAFALAKTETHVKYKDWLDSPWSGFFEGKDPLKVAPTGVKEETLIHIGNRFSSPPPNAAEFVIHKGLLRVLAARKAMVDEKVADWALGEAMAFGSLLKEGIHVRLSGQDVERGTFSHRHHVLHHQLVDKATYNSLQHMYPDQAPYSVSNSSLSEYAVLGFEHGYSMTNPNALVLWEAQFGDFSNTAQSIIDQFISSGQSKWVRQSGLVMLLPHGMEGMGPEHSSCRVERFLQMSSDDPDYFPPESDEFGVRQLHDINWIVANCSTPANYYHILRRQIALPFRKPLILCTPKSLLRHPEAKSPFSEMSEGSEFQRIIPDNGPAGQNPSNVKKVVFCSGRVYYDLTKTRREKQLEGEIAIVRVEQISPFPFDLVKEQANLYKNAELVWAQEEHKNQGSWTYVQPRFLTALNHSRDVSYVGRACGASTATGSKAQHIRELNALLNDAIST

Target ORF information:

RefSeq Version	NM_176340.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neural conserved at 73EF, transcript variant F (Nc73EF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176340.3

ORF Insert Sequence:

ATGCATAGAG CCCACACAGC CTTCAGCCTG GCCTTGTCGC CGATGGCGCA CAAGAACTTC 
GCCACATGGC TGCTCAAGAG CAGCAGCAGT CAGCAGATGG CCAAGGTAAC TGCAGCGGCC 
GCTGTGCGCA CCTACAACTC CGCAGCTGCC GAACCTTTTG CCAACGGCAG CACCGCCTCC 
TACGTGGAGG AGATGTACAA CGCCTGGCTT CGGGATCCCA CTTCCGTGCA CACCTCCTGG 
GACGCGTACT TCCGCAGCAA CAGCTACGTT AGCCCGCCCA ACTTGGCGCC CGTGCAGGCC 
AACACTCTGC CGCTGACCGC CTTCAACTTT GGAGGAGCCG TCGCCGGCGC TGCGCCCGAC 
TCCAAGACCA TTGACGACCA TCTGGCCGTC CAGGCCATCA TCAGGAGCTA CCAGTCACGA 
GGTCATTTGG CATCTGATCT GGATCCGCTT GGAATTTTGA CTCGCGAAAA AACCGTCTGC 
AAGGATGGGC TAGCACGTCG TGCTAACGAA GATGTGCTCA GGCAGCACTC TGGGTTTTTG 
TTTGGCGAGC AGGACATGGA TCGCCAGTTC AAGCTGCCCA GCACTACCTT CATCGGAGGC 
GATGAGGCTT CGTTGCCCCT AAAGGAAATC CTGAACCGCC TGGAGAATGT CTACTGTAAT 
AAGATTGGCG TGGAGTTCAT GTTCATCAAC TCTCTGGAGC AATGCAACTG GATCCGCAAG 
CGTTTTGAGA CCCCCGGCGT CCTTAACTTC TCGCCCGAGG AGAAGCGTCT GATCCTGGCC 
CGTTTGACCC GTGCCACCGG CTTTGAGGCT TTCCTGGCCA AGAAGTACTC TTCTGAGAAA 
CGTTTCGGTC TGGAGGGTTG CGAGATCATG ATCCCAGCCC TGAAGGAGAT CATCGACGTA 
TCAACGGAGT TGGGAGTGGA GTCGGTCATC ATGGGCATGC CCCATCGTGG ACGTCTTAAC 
ACCTTGGCCA ATGTATGCCG CAAGCCCTTG AACCAGATCT TCACCCAGTT CGCTGGACTG 
GAGGCTGCTG ATGATGGCTC TGGTGATGTC AAGTACCATC TCGGTACATA CATCGAGCGA 
TTGAACCGCG TTACAAACAA GAACATCCGC CTGGCTGTCG TGGCCAACCC GTCCCATCTG 
GAGGCTGTGG ATCCCGTGGT GCAGGGCAAG ACCCGTGCCG AGCAGTTCTA CCGTGGCGAC 
CAGGAGGGCA AGAAGGTTAT GTCCATTTTG ATCCACGGTG ATGCCGCCTT CTGCGGACAG 
GGCGTCGTCT ATGAAACTAT GCATCTTTCG GACCTGCCCG ACTACACCAC CCACGGAACT 
ATCCATGTGG TGGCCAACAA CCAGATCGGT TTCACCACCG ATCCCCGTTT CTCGAGGTCC 
TCTCCCTACT GTACGGATGT GGCTCGTGTC GTCAATGCTC CTATTTTCCA CGTCAACGCT 
GACGATCCAG AGGCCGTGAT GCATGTGTGC AAGGTGGCTG CTGAGTGGCG TGCTACTTTC 
CACAAGGATT GTGTTATTGA TTTGGTCGGA TACCGCCGCA ATGGACACAA CGAGATCGAC 
GAGCCCATGT TCACGCAACC CTTGATGTAC CAGAAGATCC GCAAGCACAA GAACTGTCTC 
GACCTGTATG CCGACAAACT GATCGCCGAG GGCACTGTCA CCGCCGAGGA GGTTAAGTCG 
GTTGCCGCTA AATACGAGAA CATTTGCGAG GAGGCATTCG CTCTGGCCAA GACCGAGACC 
CACGTCAAGT ACAAGGACTG GCTCGACTCG CCCTGGTCCG GCTTTTTCGA GGGCAAAGAC 
CCATTGAAGG TAGCGCCCAC TGGAGTGAAG GAGGAGACCC TCATCCACAT CGGCAACCGC 
TTCTCGTCGC CACCACCGAA CGCTGCTGAA TTCGTGATTC ACAAGGGTCT ATTGCGAGTG 
TTGGCCGCTC GCAAGGCAAT GGTGGACGAA AAGGTCGCTG ATTGGGCTTT GGGAGAGGCC 
ATGGCCTTCG GCTCTCTGCT GAAGGAGGGA ATCCATGTGC GCCTTTCGGG ACAGGATGTC 
GAGCGTGGTA CCTTCTCGCA TCGCCACCAT GTGCTGCACC ACCAGCTGGT CGACAAGGCC 
ACTTACAACT CGCTGCAGCA CATGTACCCA GATCAGGCGC CCTACTCCGT CTCCAACAGC 
TCGCTGTCGG AGTACGCAGT GCTTGGTTTC GAGCACGGTT ACTCGATGAC CAATCCCAAT 
GCCCTGGTGC TGTGGGAGGC TCAGTTCGGA GACTTCAGCA ACACGGCCCA GTCGATCATC 
GATCAGTTCA TCTCCAGCGG TCAGTCAAAG TGGGTGCGCC AATCGGGTCT GGTAATGCTC 
CTGCCCCACG GCATGGAAGG CATGGGCCCC GAGCACTCCT CGTGCCGCGT GGAGCGCTTC 
CTGCAAATGA GCAGTGACGA TCCCGACTAC TTCCCACCAG AGTCCGATGA GTTCGGAGTG 
CGACAGCTGC ACGACATTAA CTGGATCGTG GCAAACTGCT CGACGCCCGC CAACTACTAC 
CACATCCTGC GCCGACAGAT CGCATTGCCT TTCCGCAAGC CCCTGATTCT GTGCACGCCC 
AAATCGCTGC TGCGTCACCC CGAGGCTAAG AGTCCTTTCA GCGAGATGAG CGAGGGCAGC 
GAGTTCCAGC GCATCATCCC CGACAACGGT CCCGCCGGCC AGAATCCCAG CAATGTGAAG 
AAGGTCGTCT TCTGCTCGGG CCGCGTCTAC TACGATTTGA CCAAGACACG TAGGGAAAAG 
CAGCTGGAGG GCGAAATCGC CATTGTGCGC GTGGAGCAGA TCTCTCCCTT CCCCTTCGAT 
CTGGTCAAGG AGCAGGCCAA CCTGTACAAG AATGCCGAAC TCGTGTGGGC CCAAGAGGAG 
CACAAGAACC AGGGCAGCTG GACCTATGTG CAACCTCGTT TCCTCACCGC CTTGAACCAC 
AGCCGCGACG TCAGCTACGT CGGACGCGCC TGCGGAGCCT CCACCGCCAC CGGATCCAAG 
GCCCAGCACA TCCGTGAGCT GAATGCGCTG CTCAACGACG CGATTTCGAC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nc73EF cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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