MED10 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED10
Entrez Gene ID	39777
Full Name	Mediator complex subunit 10
Synonyms	BcDNA:SD24044,CG5057,Dmel\CG5057,Med10,Nut2,dMED10,dTRAP15,l(3)72Ds,med10
General protein information	Preferred Names Mediator complex subunit 10 Names CG5057 gene product from transcript CG5057-RC CG5057-PB CG5057-PC MED10-PB MED10-PC mediator mediator complex subunit 10
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 72D10-72D10

mRNA and Protein(s)

mRNA	Protein	Name
NM_140592.4	NP_648849.3	mediator complex subunit 10, isoform B
NM_001275002.1	NP_001261931.1	mediator complex subunit 10, isoform C

Pathways from BioSystems

Homologies

Homo sapiens (human)	MED10	NP_115662.2
Pan troglodytes (chimpanzee)	MED10	XP_001143506.1
Caenorhabditis elegans (roundworm)	mdt-10	NP_495650.1
Xenopus tropicalis (tropical clawed frog)	med10	NP_001004914.1
	MED10	XP_001082923.1
Mus musculus (house mouse)	Med10	NP_613062.1
Danio rerio (zebrafish)	med10	NP_001017699.1
Drosophila melanogaster (fruit fly)	MED10	NP_001261931.1
Canis lupus familiaris (dog)	MED10	XP_849860.2
Bos taurus (cattle)	MED10	NP_001029928.1
Rattus norvegicus (Norway rat)	Med10	NP_001099567.2
Gallus gallus (chicken)	MED10	XP_004935190.1
Anopheles gambiae (African malaria mosquito)	MED10_ANOGA	XP_316314.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MED10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18645	NM_001275002.1 Latest version!	Drosophila melanogaster mediator complex subunit 10, transcript variant C (MED10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa18645	NM_140592.4 Latest version!	Drosophila melanogaster mediator complex subunit 10, transcript variant B (MED10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED10 ( NM_140592.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648849.3 Drosophila melanogaster mediator complex subunit 10, transcript variant B (MED10), mRNA.

Price & Availability↑

CloneID	OFa18645
Clone ID Related Accession (Same CDS sequence)	NM_140592.4 , NM_001275002.1
Accession Version	NM_140592.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mediator complex subunit 10, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140592.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGCAC CGCTAGAGAA CCTTGAGACC CAGCTAGAGA TGTTCATCGA GAATGTTCGT 
CAGATCCGCA TAATCGTAAG CGATTTTCAG CCACAGGGTC AAAATGTACT CAACCAGAAA 
ATTAACAGCT TGGTGACCGG CCTACAAGAG ATCGACAAGC TGCGCTCCCA GGTGCAGGAC 
GTCTATGTGC CATTTGAAGT GTTTTTTGAC TACATCGACC AAGACAAGAA TCCTCAGCTG 
TACACCAAGG ATTGCGTGGA GAAGGCACTG GCCAAGAACG AGGAGGTCAA GGGCAAGATC 
GAGGGCCTGA AGAAGTTCAA AACGAATCTG CTGCTGGAGC TGTATAAAAC ATTTCCCAAT 
GAGATGAACA ACTACCGCGC TTATCGCAAG GACTCCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648849.3
CDS	119..520
Translation	MSAPLENLETQLEMFIENVRQIRIIVSDFQPQGQNVLNQKINSLVTGLQEIDKLRSQVQDVYVPFEVFFDYIDQDKNPQLYTKDCVEKALAKNEEVKGKIEGLKKFKTNLLLELYKTFPNEMNNYRAYRKDSM

Target ORF information:

RefSeq Version	NM_140592.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mediator complex subunit 10, transcript variant B (MED10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140592.4

ORF Insert Sequence:

ATGTCTGCAC CGCTAGAGAA CCTTGAGACC CAGCTAGAGA TGTTCATCGA GAATGTTCGT 
CAGATCCGCA TAATCGTAAG CGATTTTCAG CCACAGGGTC AAAATGTACT CAACCAGAAA 
ATTAACAGCT TGGTGACCGG CCTACAAGAG ATCGACAAGC TGCGCTCCCA GGTGCAGGAC 
GTCTATGTGC CATTTGAAGT GTTTTTTGAC TACATCGACC AAGACAAGAA TCCTCAGCTG 
TACACCAAGG ATTGCGTGGA GAAGGCACTG GCCAAGAACG AGGAGGTCAA GGGCAAGATC 
GAGGGCCTGA AGAAGTTCAA AACGAATCTG CTGCTGGAGC TGTATAAAAC ATTTCCCAAT 
GAGATGAACA ACTACCGCGC TTATCGCAAG GACTCCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED10 ( NM_001275002.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261931.1 Drosophila melanogaster mediator complex subunit 10, transcript variant C (MED10), mRNA.

Price & Availability↑

CloneID	OFa18645
Clone ID Related Accession (Same CDS sequence)	NM_140592.4 , NM_001275002.1
Accession Version	NM_001275002.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mediator complex subunit 10, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGCAC CGCTAGAGAA CCTTGAGACC CAGCTAGAGA TGTTCATCGA GAATGTTCGT 
CAGATCCGCA TAATCGTAAG CGATTTTCAG CCACAGGGTC AAAATGTACT CAACCAGAAA 
ATTAACAGCT TGGTGACCGG CCTACAAGAG ATCGACAAGC TGCGCTCCCA GGTGCAGGAC 
GTCTATGTGC CATTTGAAGT GTTTTTTGAC TACATCGACC AAGACAAGAA TCCTCAGCTG 
TACACCAAGG ATTGCGTGGA GAAGGCACTG GCCAAGAACG AGGAGGTCAA GGGCAAGATC 
GAGGGCCTGA AGAAGTTCAA AACGAATCTG CTGCTGGAGC TGTATAAAAC ATTTCCCAAT 
GAGATGAACA ACTACCGCGC TTATCGCAAG GACTCCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261931.1
CDS	119..520
Translation	MSAPLENLETQLEMFIENVRQIRIIVSDFQPQGQNVLNQKINSLVTGLQEIDKLRSQVQDVYVPFEVFFDYIDQDKNPQLYTKDCVEKALAKNEEVKGKIEGLKKFKTNLLLELYKTFPNEMNNYRAYRKDSM

Target ORF information:

RefSeq Version	NM_001275002.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mediator complex subunit 10, transcript variant C (MED10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275002.1

ORF Insert Sequence:

ATGTCTGCAC CGCTAGAGAA CCTTGAGACC CAGCTAGAGA TGTTCATCGA GAATGTTCGT 
CAGATCCGCA TAATCGTAAG CGATTTTCAG CCACAGGGTC AAAATGTACT CAACCAGAAA 
ATTAACAGCT TGGTGACCGG CCTACAAGAG ATCGACAAGC TGCGCTCCCA GGTGCAGGAC 
GTCTATGTGC CATTTGAAGT GTTTTTTGAC TACATCGACC AAGACAAGAA TCCTCAGCTG 
TACACCAAGG ATTGCGTGGA GAAGGCACTG GCCAAGAACG AGGAGGTCAA GGGCAAGATC 
GAGGGCCTGA AGAAGTTCAA AACGAATCTG CTGCTGGAGC TGTATAAAAC ATTTCCCAAT 
GAGATGAACA ACTACCGCGC TTATCGCAAG GACTCCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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MED10 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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