CG12316 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG12316
Entrez Gene ID	39650
Full Name	CG12316 gene product from transcript CG12316-RA
Synonyms	Dmel\CG12316
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 71B5-71B5

mRNA and Protein(s)

mRNA	Protein	Name
NM_168611.1	NP_730030.1	uncharacterized protein, isoform B
NM_140495.2	NP_648752.1	uncharacterized protein, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG12316 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG12316 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa20238	NM_168611.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG12316), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa20238	NM_140495.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG12316), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG12316 ( NM_168611.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730030.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG12316), mRNA.

Price & Availability↑

CloneID	OFa20238
Clone ID Related Accession (Same CDS sequence)	NM_168611.1 , NM_140495.2
Accession Version	NM_168611.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCTGCT TGGTCGGGAA CCGAAAGCGG AACAGCGGCA GCACGGCGGA CGAATTCGAC 
AAGGTCAGCA CTTTGGCCAA TGCCGATGAT ACCGAGTGCG TATCGCCGGA TCCGGGGCGG 
GAGCCGGAAA GCAACAAACC GGATTCGCAG ACGGAGCCTG AGTTTGGCCA TTTGAATGAG 
ACGCGGAGGA AGATTCTCGA TGCCATTGCT GCTTTAAATG ATGATAGCTT CGATAAGCGC 
ATGGCCCGGT TGCTCATGCA GTACGAGGGC GCCATTCGGG AAGAGTTTCA GCGGGCACAG 
TATTTGCCCC AGAAGTGCCG CAAGCTGGCG CGAATCCTAA CCGGCCTCAC TGATGTCATC 
CACACTATGG TTCAGGGTCG AGCGCTGCAG CGGGACGAGA GCTACAGGCA ACGCATGTGC 
AATATTGTTG GTTTCTACAT ATCCTGCGAA CTCTGTTCCG CTCAGGAGCC GGTGGAGAAG 
GAGGAGACCA TCATCAACAG GATTAACAGC TGCCTGAAGC TCTACGTGGA GTACGGAGAT 
GGTGGCGCCG GCGTCCACCG GGAGCACGTG CTGCGCACTC TCCTCGCTGC ACCCAAGCTC 
TTCAACAGAG CTAGCATTTT GTCCATGCTG TACAGCCGCC TCTTTCCCCA CTGGTCCGTT 
CCCAGCGTTA TGATACAAGC AGAACTGCCA GATAAACTCT ACATTGAATA CATACTCATA 
TTTTACTACT GGCAGCGTCT GGAGTCGGAT GAGTCAGTCA AGGAGCGCAT CGTGCAGTTC 
GCCGAAAAGT TCATGAGGCC CTCAAGATCA CTGGCCATAA AAAGTGTCTA TGCTCATTAC 
TTGCCAAAGT TTACGGACCA GAATGCCGCC ACGCGGACCA TATTGAATCA TCTGCGAATG 
AGCAGTTGCA CAAGTTTGCA TGTGGTCAGC AAGGTGGACA ATCGACCGCC CCAGTCCAAC 
GAAGTGGTTC TCAGTTCTGA TGATGAGGAC AGCTGCCCGA TGTGGGACAT ATCGGGTACG 
CCGACATCAT CAATTTACAA TCATCCGCCC GTTTTCCTCC AGAATCTCTG TCAGAATGCT 
CGCCAGTTGG AGCCGTGCAA GCGTGCCAAC GTTTTGTTTA GCGGCATTGA CAACTCCATG 
GAGATTGTAG AGCTACGCGA CTCTGACGAT GACGTAGAGA TGTTTTCGGT GAACTTTAAT 
GTGCCTGCCA ACGTAGATGG CATCAACTCG AACTCCAACT CAAACGACTC AGCTAATCAG 
ACATCGACAA CGATCCTCTG CGATGACGAC TCCGTGCCCG TCACTCGAAT CTATCCGAGC 
AATCTGCGGA CTTATGAAAG GACTGTGCTT CCACCCACTA CCTACACAGT TCCGCGGATT 
GTGAACAGCT ATAGTTGTCG CCGGGATAGT CTCCATTTGG TGTCTACCAC GGCGCCACAT 
CTTGTCGATC AGTGTGTGCA AACGTTGGAC GACGAGGAGC AGGATCTGGT AGAGGAGGAC 
CAATACTCAG GCGCCCACAG CCACGGCTTC TTCCCACGAA GCAGTAATGC CAGCTATGCC 
AGCGGACAAC GAACCCCACT TTACCACTGC CGTCAAACTT CTAGTCAAAA CAGCAACTGC 
AGCGAGTCTT CGCTGCTAAA GAAACAGGTT ACCTTCAGTC CCCGGCACTT GGGCGCGGCA 
ACTCCGAACG CCAAGACAGG CACATCCAAG AAGCCATCCA TTAAGCAGTA CAAGCCATCT 
TCGTCCAATG CCACAGAATC ACAACGATGG AAAGAGGAGC ACCGAAAGGC GAGTGCGAAA 
ATGTTTGCCA AACTGGACGC CAAGATCAAA GCCAGAAGTG AAGCTGAAAA TGTGTCCACC 
AGCCAGTTAA CGCCCACCAC TAGCAACGCC TCCAGTAGTA CACCCACAAA GATCTCGGGC 
ACACCCACCC GCATCCGGAG TACGCAACGA GGACAAAGAG TGGCGTTGCC CACGCCGCCA 
GCTTCCACGC ACAGTTCGAG TAGTCCCTGC CAGATACGAA GCGCATCAAC TGATAACCAT 
AACTACAACG TCGATTCGGA TTACAGTCGC CGCTGTTTCT CCAAGGCAGC TGGCGATGAA 
GTGAGCTACT ACAATCACCT GCTAACCCTG CAGCGCGAGA ATATCCGACG ACGTAGGTTG 
AATAATCAGA AAAAGAAAAA GAATCGAAAA GTCAGCCAAA AGGTGCAAAA GAAGGACGAT 
AAGCTTAATA TGCTGAAGCA GGCCACAAAG CGATGTCGGA AGCTTCGCGC CTTAAATGCG 
AAACGGAAAA ACGAGGTACA GAAACTGGAG CAGGATCTCG ACACTATTCG GAAATCTGCA 
CCGCATAAAT TGCCTATAAT AAGACTGAAA CGTTGTAATC TCAAGACCCA GAAACGGTCA 
CTCAAAGCTG AAGAAGCACC CAGTGTCCAA GAGGATATAC TTTCTATGAA CTCTGATCGA 
GAATTACAGG AACAAAAAAA AGCGCAGAAG CCTAAAAGAA AGCGATACAA AAAAGTGCAA 
AAGGAGCAAG AAAACGAAAA ATATTTGCGG GAACAAGAAA TTATAATATC GCAAAGCCAA 
ATGGTTGAGG AAGATAAAGT GGAGGAACAC CACGCTCACG AAGCGCCAAG TGAGCAGCAA 
CGAGAGCTCG ATCTGGAGCA TCAACAATTT CTGGAAGAGC AGCTGCTACA GGAGGAACAT 
CAATTGGCGA TGCACGAGGA AGAGCATCAC CAAATGGATG ATGAACAAGA GTCACACGAA 
GTAGCTCGTC TGCTAGAAGA TCAGCAGCCG GCGGAGGGAA CACATCTTCA TGTTGAGAAT 
GAAATGTTGG CAGAGAAAGA GCAACCTCTA ATAGAAGATG ATGACCAGGA AGCGGATGAT 
CAAAATCAAA ATGAGCACTT ATCGGATGAT CAGCAACAGG AAGCGGATCA ACAGCTGTCT 
ATAGAGCAGC CTCAGGAGCA CGAAGAGAAT CAGTCACTCA GCCAAGATCC GTTAGACGAA 
CAATGTACGC CCACACCGAA CGGCAAAAGG CGCAAGCGAA GACGGCGATT CAAGCGACGT 
TGTTGGACGC ACGTAAAGAA TAAGAAAAAG AAGCAGTGGC TAATAGATTA CCCATTGGAG 
TTTGAGGCAG ATCCCGCGTT AGATACCCCT CCTATTCAAC CAGAGCCAGC TCCAGATGAA 
CAACAAGCCG AGCTAGTTAC CGGGCCAGTT TCCGAGTCAG TTGCTGAGCC AGTTGCTGAG 
CCAGTTGCCG AGCCAGTTTC TGAGCCATTT GCAGAGCAAA TTGCCGAGCC AATTGCCCAG 
CCGACACCAG AACCCGCCTA CGATTTCGAT CAGGACTTGG TGCCCGAGGT GGGCAATGAA 
TTGGAGATTG CTGCTCCCAT TCGCGTCGCC GAGGACGAAG ACAGCACTGC GATACTCTCG 
CCGTCCCACA ACCTGGAACT GGTGACCAGC CACGTGCCGG TTTACTTGCT CAATGGAGAA 
AGTCGCGATG TCAGCACACC CCTGTCCAGC AGCTCTTACA GTGCCGGGGG GTCAGAGATA 
TATGCGTCGT CGAAGGATTT AGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730030.1
CDS	58..3624
Translation	MSCLVGNRKRNSGSTADEFDKVSTLANADDTECVSPDPGREPESNKPDSQTEPEFGHLNETRRKILDAIAALNDDSFDKRMARLLMQYEGAIREEFQRAQYLPQKCRKLARILTGLTDVIHTMVQGRALQRDESYRQRMCNIVGFYISCELCSAQEPVEKEETIINRINSCLKLYVEYGDGGAGVHREHVLRTLLAAPKLFNRASILSMLYSRLFPHWSVPSVMIQAELPDKLYIEYILIFYYWQRLESDESVKERIVQFAEKFMRPSRSLAIKSVYAHYLPKFTDQNAATRTILNHLRMSSCTSLHVVSKVDNRPPQSNEVVLSSDDEDSCPMWDISGTPTSSIYNHPPVFLQNLCQNARQLEPCKRANVLFSGIDNSMEIVELRDSDDDVEMFSVNFNVPANVDGINSNSNSNDSANQTSTTILCDDDSVPVTRIYPSNLRTYERTVLPPTTYTVPRIVNSYSCRRDSLHLVSTTAPHLVDQCVQTLDDEEQDLVEEDQYSGAHSHGFFPRSSNASYASGQRTPLYHCRQTSSQNSNCSESSLLKKQVTFSPRHLGAATPNAKTGTSKKPSIKQYKPSSSNATESQRWKEEHRKASAKMFAKLDAKIKARSEAENVSTSQLTPTTSNASSSTPTKISGTPTRIRSTQRGQRVALPTPPASTHSSSSPCQIRSASTDNHNYNVDSDYSRRCFSKAAGDEVSYYNHLLTLQRENIRRRRLNNQKKKKNRKVSQKVQKKDDKLNMLKQATKRCRKLRALNAKRKNEVQKLEQDLDTIRKSAPHKLPIIRLKRCNLKTQKRSLKAEEAPSVQEDILSMNSDRELQEQKKAQKPKRKRYKKVQKEQENEKYLREQEIIISQSQMVEEDKVEEHHAHEAPSEQQRELDLEHQQFLEEQLLQEEHQLAMHEEEHHQMDDEQESHEVARLLEDQQPAEGTHLHVENEMLAEKEQPLIEDDDQEADDQNQNEHLSDDQQQEADQQLSIEQPQEHEENQSLSQDPLDEQCTPTPNGKRRKRRRRFKRRCWTHVKNKKKKQWLIDYPLEFEADPALDTPPIQPEPAPDEQQAELVTGPVSESVAEPVAEPVAEPVSEPFAEQIAEPIAQPTPEPAYDFDQDLVPEVGNELEIAAPIRVAEDEDSTAILSPSHNLELVTSHVPVYLLNGESRDVSTPLSSSSYSAGGSEIYASSKDLV

Target ORF information:

RefSeq Version	NM_168611.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG12316), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168611.1

ORF Insert Sequence:

ATGAGCTGCT TGGTCGGGAA CCGAAAGCGG AACAGCGGCA GCACGGCGGA CGAATTCGAC 
AAGGTCAGCA CTTTGGCCAA TGCCGATGAT ACCGAGTGCG TATCGCCGGA TCCGGGGCGG 
GAGCCGGAAA GCAACAAACC GGATTCGCAG ACGGAGCCTG AGTTTGGCCA TTTGAATGAG 
ACGCGGAGGA AGATTCTCGA TGCCATTGCT GCTTTAAATG ATGATAGCTT CGATAAGCGC 
ATGGCCCGGT TGCTCATGCA GTACGAGGGC GCCATTCGGG AAGAGTTTCA GCGGGCACAG 
TATTTGCCCC AGAAGTGCCG CAAGCTGGCG CGAATCCTAA CCGGCCTCAC TGATGTCATC 
CACACTATGG TTCAGGGTCG AGCGCTGCAG CGGGACGAGA GCTACAGGCA ACGCATGTGC 
AATATTGTTG GTTTCTACAT ATCCTGCGAA CTCTGTTCCG CTCAGGAGCC GGTGGAGAAG 
GAGGAGACCA TCATCAACAG GATTAACAGC TGCCTGAAGC TCTACGTGGA GTACGGAGAT 
GGTGGCGCCG GCGTCCACCG GGAGCACGTG CTGCGCACTC TCCTCGCTGC ACCCAAGCTC 
TTCAACAGAG CTAGCATTTT GTCCATGCTG TACAGCCGCC TCTTTCCCCA CTGGTCCGTT 
CCCAGCGTTA TGATACAAGC AGAACTGCCA GATAAACTCT ACATTGAATA CATACTCATA 
TTTTACTACT GGCAGCGTCT GGAGTCGGAT GAGTCAGTCA AGGAGCGCAT CGTGCAGTTC 
GCCGAAAAGT TCATGAGGCC CTCAAGATCA CTGGCCATAA AAAGTGTCTA TGCTCATTAC 
TTGCCAAAGT TTACGGACCA GAATGCCGCC ACGCGGACCA TATTGAATCA TCTGCGAATG 
AGCAGTTGCA CAAGTTTGCA TGTGGTCAGC AAGGTGGACA ATCGACCGCC CCAGTCCAAC 
GAAGTGGTTC TCAGTTCTGA TGATGAGGAC AGCTGCCCGA TGTGGGACAT ATCGGGTACG 
CCGACATCAT CAATTTACAA TCATCCGCCC GTTTTCCTCC AGAATCTCTG TCAGAATGCT 
CGCCAGTTGG AGCCGTGCAA GCGTGCCAAC GTTTTGTTTA GCGGCATTGA CAACTCCATG 
GAGATTGTAG AGCTACGCGA CTCTGACGAT GACGTAGAGA TGTTTTCGGT GAACTTTAAT 
GTGCCTGCCA ACGTAGATGG CATCAACTCG AACTCCAACT CAAACGACTC AGCTAATCAG 
ACATCGACAA CGATCCTCTG CGATGACGAC TCCGTGCCCG TCACTCGAAT CTATCCGAGC 
AATCTGCGGA CTTATGAAAG GACTGTGCTT CCACCCACTA CCTACACAGT TCCGCGGATT 
GTGAACAGCT ATAGTTGTCG CCGGGATAGT CTCCATTTGG TGTCTACCAC GGCGCCACAT 
CTTGTCGATC AGTGTGTGCA AACGTTGGAC GACGAGGAGC AGGATCTGGT AGAGGAGGAC 
CAATACTCAG GCGCCCACAG CCACGGCTTC TTCCCACGAA GCAGTAATGC CAGCTATGCC 
AGCGGACAAC GAACCCCACT TTACCACTGC CGTCAAACTT CTAGTCAAAA CAGCAACTGC 
AGCGAGTCTT CGCTGCTAAA GAAACAGGTT ACCTTCAGTC CCCGGCACTT GGGCGCGGCA 
ACTCCGAACG CCAAGACAGG CACATCCAAG AAGCCATCCA TTAAGCAGTA CAAGCCATCT 
TCGTCCAATG CCACAGAATC ACAACGATGG AAAGAGGAGC ACCGAAAGGC GAGTGCGAAA 
ATGTTTGCCA AACTGGACGC CAAGATCAAA GCCAGAAGTG AAGCTGAAAA TGTGTCCACC 
AGCCAGTTAA CGCCCACCAC TAGCAACGCC TCCAGTAGTA CACCCACAAA GATCTCGGGC 
ACACCCACCC GCATCCGGAG TACGCAACGA GGACAAAGAG TGGCGTTGCC CACGCCGCCA 
GCTTCCACGC ACAGTTCGAG TAGTCCCTGC CAGATACGAA GCGCATCAAC TGATAACCAT 
AACTACAACG TCGATTCGGA TTACAGTCGC CGCTGTTTCT CCAAGGCAGC TGGCGATGAA 
GTGAGCTACT ACAATCACCT GCTAACCCTG CAGCGCGAGA ATATCCGACG ACGTAGGTTG 
AATAATCAGA AAAAGAAAAA GAATCGAAAA GTCAGCCAAA AGGTGCAAAA GAAGGACGAT 
AAGCTTAATA TGCTGAAGCA GGCCACAAAG CGATGTCGGA AGCTTCGCGC CTTAAATGCG 
AAACGGAAAA ACGAGGTACA GAAACTGGAG CAGGATCTCG ACACTATTCG GAAATCTGCA 
CCGCATAAAT TGCCTATAAT AAGACTGAAA CGTTGTAATC TCAAGACCCA GAAACGGTCA 
CTCAAAGCTG AAGAAGCACC CAGTGTCCAA GAGGATATAC TTTCTATGAA CTCTGATCGA 
GAATTACAGG AACAAAAAAA AGCGCAGAAG CCTAAAAGAA AGCGATACAA AAAAGTGCAA 
AAGGAGCAAG AAAACGAAAA ATATTTGCGG GAACAAGAAA TTATAATATC GCAAAGCCAA 
ATGGTTGAGG AAGATAAAGT GGAGGAACAC CACGCTCACG AAGCGCCAAG TGAGCAGCAA 
CGAGAGCTCG ATCTGGAGCA TCAACAATTT CTGGAAGAGC AGCTGCTACA GGAGGAACAT 
CAATTGGCGA TGCACGAGGA AGAGCATCAC CAAATGGATG ATGAACAAGA GTCACACGAA 
GTAGCTCGTC TGCTAGAAGA TCAGCAGCCG GCGGAGGGAA CACATCTTCA TGTTGAGAAT 
GAAATGTTGG CAGAGAAAGA GCAACCTCTA ATAGAAGATG ATGACCAGGA AGCGGATGAT 
CAAAATCAAA ATGAGCACTT ATCGGATGAT CAGCAACAGG AAGCGGATCA ACAGCTGTCT 
ATAGAGCAGC CTCAGGAGCA CGAAGAGAAT CAGTCACTCA GCCAAGATCC GTTAGACGAA 
CAATGTACGC CCACACCGAA CGGCAAAAGG CGCAAGCGAA GACGGCGATT CAAGCGACGT 
TGTTGGACGC ACGTAAAGAA TAAGAAAAAG AAGCAGTGGC TAATAGATTA CCCATTGGAG 
TTTGAGGCAG ATCCCGCGTT AGATACCCCT CCTATTCAAC CAGAGCCAGC TCCAGATGAA 
CAACAAGCCG AGCTAGTTAC CGGGCCAGTT TCCGAGTCAG TTGCTGAGCC AGTTGCTGAG 
CCAGTTGCCG AGCCAGTTTC TGAGCCATTT GCAGAGCAAA TTGCCGAGCC AATTGCCCAG 
CCGACACCAG AACCCGCCTA CGATTTCGAT CAGGACTTGG TGCCCGAGGT GGGCAATGAA 
TTGGAGATTG CTGCTCCCAT TCGCGTCGCC GAGGACGAAG ACAGCACTGC GATACTCTCG 
CCGTCCCACA ACCTGGAACT GGTGACCAGC CACGTGCCGG TTTACTTGCT CAATGGAGAA 
AGTCGCGATG TCAGCACACC CCTGTCCAGC AGCTCTTACA GTGCCGGGGG GTCAGAGATA 
TATGCGTCGT CGAAGGATTT AGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG12316 ( NM_140495.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648752.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG12316), mRNA.

Price & Availability↑

CloneID	OFa20238
Clone ID Related Accession (Same CDS sequence)	NM_168611.1 , NM_140495.2
Accession Version	NM_140495.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140495.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCTGCT TGGTCGGGAA CCGAAAGCGG AACAGCGGCA GCACGGCGGA CGAATTCGAC 
AAGGTCAGCA CTTTGGCCAA TGCCGATGAT ACCGAGTGCG TATCGCCGGA TCCGGGGCGG 
GAGCCGGAAA GCAACAAACC GGATTCGCAG ACGGAGCCTG AGTTTGGCCA TTTGAATGAG 
ACGCGGAGGA AGATTCTCGA TGCCATTGCT GCTTTAAATG ATGATAGCTT CGATAAGCGC 
ATGGCCCGGT TGCTCATGCA GTACGAGGGC GCCATTCGGG AAGAGTTTCA GCGGGCACAG 
TATTTGCCCC AGAAGTGCCG CAAGCTGGCG CGAATCCTAA CCGGCCTCAC TGATGTCATC 
CACACTATGG TTCAGGGTCG AGCGCTGCAG CGGGACGAGA GCTACAGGCA ACGCATGTGC 
AATATTGTTG GTTTCTACAT ATCCTGCGAA CTCTGTTCCG CTCAGGAGCC GGTGGAGAAG 
GAGGAGACCA TCATCAACAG GATTAACAGC TGCCTGAAGC TCTACGTGGA GTACGGAGAT 
GGTGGCGCCG GCGTCCACCG GGAGCACGTG CTGCGCACTC TCCTCGCTGC ACCCAAGCTC 
TTCAACAGAG CTAGCATTTT GTCCATGCTG TACAGCCGCC TCTTTCCCCA CTGGTCCGTT 
CCCAGCGTTA TGATACAAGC AGAACTGCCA GATAAACTCT ACATTGAATA CATACTCATA 
TTTTACTACT GGCAGCGTCT GGAGTCGGAT GAGTCAGTCA AGGAGCGCAT CGTGCAGTTC 
GCCGAAAAGT TCATGAGGCC CTCAAGATCA CTGGCCATAA AAAGTGTCTA TGCTCATTAC 
TTGCCAAAGT TTACGGACCA GAATGCCGCC ACGCGGACCA TATTGAATCA TCTGCGAATG 
AGCAGTTGCA CAAGTTTGCA TGTGGTCAGC AAGGTGGACA ATCGACCGCC CCAGTCCAAC 
GAAGTGGTTC TCAGTTCTGA TGATGAGGAC AGCTGCCCGA TGTGGGACAT ATCGGGTACG 
CCGACATCAT CAATTTACAA TCATCCGCCC GTTTTCCTCC AGAATCTCTG TCAGAATGCT 
CGCCAGTTGG AGCCGTGCAA GCGTGCCAAC GTTTTGTTTA GCGGCATTGA CAACTCCATG 
GAGATTGTAG AGCTACGCGA CTCTGACGAT GACGTAGAGA TGTTTTCGGT GAACTTTAAT 
GTGCCTGCCA ACGTAGATGG CATCAACTCG AACTCCAACT CAAACGACTC AGCTAATCAG 
ACATCGACAA CGATCCTCTG CGATGACGAC TCCGTGCCCG TCACTCGAAT CTATCCGAGC 
AATCTGCGGA CTTATGAAAG GACTGTGCTT CCACCCACTA CCTACACAGT TCCGCGGATT 
GTGAACAGCT ATAGTTGTCG CCGGGATAGT CTCCATTTGG TGTCTACCAC GGCGCCACAT 
CTTGTCGATC AGTGTGTGCA AACGTTGGAC GACGAGGAGC AGGATCTGGT AGAGGAGGAC 
CAATACTCAG GCGCCCACAG CCACGGCTTC TTCCCACGAA GCAGTAATGC CAGCTATGCC 
AGCGGACAAC GAACCCCACT TTACCACTGC CGTCAAACTT CTAGTCAAAA CAGCAACTGC 
AGCGAGTCTT CGCTGCTAAA GAAACAGGTT ACCTTCAGTC CCCGGCACTT GGGCGCGGCA 
ACTCCGAACG CCAAGACAGG CACATCCAAG AAGCCATCCA TTAAGCAGTA CAAGCCATCT 
TCGTCCAATG CCACAGAATC ACAACGATGG AAAGAGGAGC ACCGAAAGGC GAGTGCGAAA 
ATGTTTGCCA AACTGGACGC CAAGATCAAA GCCAGAAGTG AAGCTGAAAA TGTGTCCACC 
AGCCAGTTAA CGCCCACCAC TAGCAACGCC TCCAGTAGTA CACCCACAAA GATCTCGGGC 
ACACCCACCC GCATCCGGAG TACGCAACGA GGACAAAGAG TGGCGTTGCC CACGCCGCCA 
GCTTCCACGC ACAGTTCGAG TAGTCCCTGC CAGATACGAA GCGCATCAAC TGATAACCAT 
AACTACAACG TCGATTCGGA TTACAGTCGC CGCTGTTTCT CCAAGGCAGC TGGCGATGAA 
GTGAGCTACT ACAATCACCT GCTAACCCTG CAGCGCGAGA ATATCCGACG ACGTAGGTTG 
AATAATCAGA AAAAGAAAAA GAATCGAAAA GTCAGCCAAA AGGTGCAAAA GAAGGACGAT 
AAGCTTAATA TGCTGAAGCA GGCCACAAAG CGATGTCGGA AGCTTCGCGC CTTAAATGCG 
AAACGGAAAA ACGAGGTACA GAAACTGGAG CAGGATCTCG ACACTATTCG GAAATCTGCA 
CCGCATAAAT TGCCTATAAT AAGACTGAAA CGTTGTAATC TCAAGACCCA GAAACGGTCA 
CTCAAAGCTG AAGAAGCACC CAGTGTCCAA GAGGATATAC TTTCTATGAA CTCTGATCGA 
GAATTACAGG AACAAAAAAA AGCGCAGAAG CCTAAAAGAA AGCGATACAA AAAAGTGCAA 
AAGGAGCAAG AAAACGAAAA ATATTTGCGG GAACAAGAAA TTATAATATC GCAAAGCCAA 
ATGGTTGAGG AAGATAAAGT GGAGGAACAC CACGCTCACG AAGCGCCAAG TGAGCAGCAA 
CGAGAGCTCG ATCTGGAGCA TCAACAATTT CTGGAAGAGC AGCTGCTACA GGAGGAACAT 
CAATTGGCGA TGCACGAGGA AGAGCATCAC CAAATGGATG ATGAACAAGA GTCACACGAA 
GTAGCTCGTC TGCTAGAAGA TCAGCAGCCG GCGGAGGGAA CACATCTTCA TGTTGAGAAT 
GAAATGTTGG CAGAGAAAGA GCAACCTCTA ATAGAAGATG ATGACCAGGA AGCGGATGAT 
CAAAATCAAA ATGAGCACTT ATCGGATGAT CAGCAACAGG AAGCGGATCA ACAGCTGTCT 
ATAGAGCAGC CTCAGGAGCA CGAAGAGAAT CAGTCACTCA GCCAAGATCC GTTAGACGAA 
CAATGTACGC CCACACCGAA CGGCAAAAGG CGCAAGCGAA GACGGCGATT CAAGCGACGT 
TGTTGGACGC ACGTAAAGAA TAAGAAAAAG AAGCAGTGGC TAATAGATTA CCCATTGGAG 
TTTGAGGCAG ATCCCGCGTT AGATACCCCT CCTATTCAAC CAGAGCCAGC TCCAGATGAA 
CAACAAGCCG AGCTAGTTAC CGGGCCAGTT TCCGAGTCAG TTGCTGAGCC AGTTGCTGAG 
CCAGTTGCCG AGCCAGTTTC TGAGCCATTT GCAGAGCAAA TTGCCGAGCC AATTGCCCAG 
CCGACACCAG AACCCGCCTA CGATTTCGAT CAGGACTTGG TGCCCGAGGT GGGCAATGAA 
TTGGAGATTG CTGCTCCCAT TCGCGTCGCC GAGGACGAAG ACAGCACTGC GATACTCTCG 
CCGTCCCACA ACCTGGAACT GGTGACCAGC CACGTGCCGG TTTACTTGCT CAATGGAGAA 
AGTCGCGATG TCAGCACACC CCTGTCCAGC AGCTCTTACA GTGCCGGGGG GTCAGAGATA 
TATGCGTCGT CGAAGGATTT AGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648752.1
CDS	466..4032
Translation	MSCLVGNRKRNSGSTADEFDKVSTLANADDTECVSPDPGREPESNKPDSQTEPEFGHLNETRRKILDAIAALNDDSFDKRMARLLMQYEGAIREEFQRAQYLPQKCRKLARILTGLTDVIHTMVQGRALQRDESYRQRMCNIVGFYISCELCSAQEPVEKEETIINRINSCLKLYVEYGDGGAGVHREHVLRTLLAAPKLFNRASILSMLYSRLFPHWSVPSVMIQAELPDKLYIEYILIFYYWQRLESDESVKERIVQFAEKFMRPSRSLAIKSVYAHYLPKFTDQNAATRTILNHLRMSSCTSLHVVSKVDNRPPQSNEVVLSSDDEDSCPMWDISGTPTSSIYNHPPVFLQNLCQNARQLEPCKRANVLFSGIDNSMEIVELRDSDDDVEMFSVNFNVPANVDGINSNSNSNDSANQTSTTILCDDDSVPVTRIYPSNLRTYERTVLPPTTYTVPRIVNSYSCRRDSLHLVSTTAPHLVDQCVQTLDDEEQDLVEEDQYSGAHSHGFFPRSSNASYASGQRTPLYHCRQTSSQNSNCSESSLLKKQVTFSPRHLGAATPNAKTGTSKKPSIKQYKPSSSNATESQRWKEEHRKASAKMFAKLDAKIKARSEAENVSTSQLTPTTSNASSSTPTKISGTPTRIRSTQRGQRVALPTPPASTHSSSSPCQIRSASTDNHNYNVDSDYSRRCFSKAAGDEVSYYNHLLTLQRENIRRRRLNNQKKKKNRKVSQKVQKKDDKLNMLKQATKRCRKLRALNAKRKNEVQKLEQDLDTIRKSAPHKLPIIRLKRCNLKTQKRSLKAEEAPSVQEDILSMNSDRELQEQKKAQKPKRKRYKKVQKEQENEKYLREQEIIISQSQMVEEDKVEEHHAHEAPSEQQRELDLEHQQFLEEQLLQEEHQLAMHEEEHHQMDDEQESHEVARLLEDQQPAEGTHLHVENEMLAEKEQPLIEDDDQEADDQNQNEHLSDDQQQEADQQLSIEQPQEHEENQSLSQDPLDEQCTPTPNGKRRKRRRRFKRRCWTHVKNKKKKQWLIDYPLEFEADPALDTPPIQPEPAPDEQQAELVTGPVSESVAEPVAEPVAEPVSEPFAEQIAEPIAQPTPEPAYDFDQDLVPEVGNELEIAAPIRVAEDEDSTAILSPSHNLELVTSHVPVYLLNGESRDVSTPLSSSSYSAGGSEIYASSKDLV

Target ORF information:

RefSeq Version	NM_140495.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG12316), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140495.2

ORF Insert Sequence:

ATGAGCTGCT TGGTCGGGAA CCGAAAGCGG AACAGCGGCA GCACGGCGGA CGAATTCGAC 
AAGGTCAGCA CTTTGGCCAA TGCCGATGAT ACCGAGTGCG TATCGCCGGA TCCGGGGCGG 
GAGCCGGAAA GCAACAAACC GGATTCGCAG ACGGAGCCTG AGTTTGGCCA TTTGAATGAG 
ACGCGGAGGA AGATTCTCGA TGCCATTGCT GCTTTAAATG ATGATAGCTT CGATAAGCGC 
ATGGCCCGGT TGCTCATGCA GTACGAGGGC GCCATTCGGG AAGAGTTTCA GCGGGCACAG 
TATTTGCCCC AGAAGTGCCG CAAGCTGGCG CGAATCCTAA CCGGCCTCAC TGATGTCATC 
CACACTATGG TTCAGGGTCG AGCGCTGCAG CGGGACGAGA GCTACAGGCA ACGCATGTGC 
AATATTGTTG GTTTCTACAT ATCCTGCGAA CTCTGTTCCG CTCAGGAGCC GGTGGAGAAG 
GAGGAGACCA TCATCAACAG GATTAACAGC TGCCTGAAGC TCTACGTGGA GTACGGAGAT 
GGTGGCGCCG GCGTCCACCG GGAGCACGTG CTGCGCACTC TCCTCGCTGC ACCCAAGCTC 
TTCAACAGAG CTAGCATTTT GTCCATGCTG TACAGCCGCC TCTTTCCCCA CTGGTCCGTT 
CCCAGCGTTA TGATACAAGC AGAACTGCCA GATAAACTCT ACATTGAATA CATACTCATA 
TTTTACTACT GGCAGCGTCT GGAGTCGGAT GAGTCAGTCA AGGAGCGCAT CGTGCAGTTC 
GCCGAAAAGT TCATGAGGCC CTCAAGATCA CTGGCCATAA AAAGTGTCTA TGCTCATTAC 
TTGCCAAAGT TTACGGACCA GAATGCCGCC ACGCGGACCA TATTGAATCA TCTGCGAATG 
AGCAGTTGCA CAAGTTTGCA TGTGGTCAGC AAGGTGGACA ATCGACCGCC CCAGTCCAAC 
GAAGTGGTTC TCAGTTCTGA TGATGAGGAC AGCTGCCCGA TGTGGGACAT ATCGGGTACG 
CCGACATCAT CAATTTACAA TCATCCGCCC GTTTTCCTCC AGAATCTCTG TCAGAATGCT 
CGCCAGTTGG AGCCGTGCAA GCGTGCCAAC GTTTTGTTTA GCGGCATTGA CAACTCCATG 
GAGATTGTAG AGCTACGCGA CTCTGACGAT GACGTAGAGA TGTTTTCGGT GAACTTTAAT 
GTGCCTGCCA ACGTAGATGG CATCAACTCG AACTCCAACT CAAACGACTC AGCTAATCAG 
ACATCGACAA CGATCCTCTG CGATGACGAC TCCGTGCCCG TCACTCGAAT CTATCCGAGC 
AATCTGCGGA CTTATGAAAG GACTGTGCTT CCACCCACTA CCTACACAGT TCCGCGGATT 
GTGAACAGCT ATAGTTGTCG CCGGGATAGT CTCCATTTGG TGTCTACCAC GGCGCCACAT 
CTTGTCGATC AGTGTGTGCA AACGTTGGAC GACGAGGAGC AGGATCTGGT AGAGGAGGAC 
CAATACTCAG GCGCCCACAG CCACGGCTTC TTCCCACGAA GCAGTAATGC CAGCTATGCC 
AGCGGACAAC GAACCCCACT TTACCACTGC CGTCAAACTT CTAGTCAAAA CAGCAACTGC 
AGCGAGTCTT CGCTGCTAAA GAAACAGGTT ACCTTCAGTC CCCGGCACTT GGGCGCGGCA 
ACTCCGAACG CCAAGACAGG CACATCCAAG AAGCCATCCA TTAAGCAGTA CAAGCCATCT 
TCGTCCAATG CCACAGAATC ACAACGATGG AAAGAGGAGC ACCGAAAGGC GAGTGCGAAA 
ATGTTTGCCA AACTGGACGC CAAGATCAAA GCCAGAAGTG AAGCTGAAAA TGTGTCCACC 
AGCCAGTTAA CGCCCACCAC TAGCAACGCC TCCAGTAGTA CACCCACAAA GATCTCGGGC 
ACACCCACCC GCATCCGGAG TACGCAACGA GGACAAAGAG TGGCGTTGCC CACGCCGCCA 
GCTTCCACGC ACAGTTCGAG TAGTCCCTGC CAGATACGAA GCGCATCAAC TGATAACCAT 
AACTACAACG TCGATTCGGA TTACAGTCGC CGCTGTTTCT CCAAGGCAGC TGGCGATGAA 
GTGAGCTACT ACAATCACCT GCTAACCCTG CAGCGCGAGA ATATCCGACG ACGTAGGTTG 
AATAATCAGA AAAAGAAAAA GAATCGAAAA GTCAGCCAAA AGGTGCAAAA GAAGGACGAT 
AAGCTTAATA TGCTGAAGCA GGCCACAAAG CGATGTCGGA AGCTTCGCGC CTTAAATGCG 
AAACGGAAAA ACGAGGTACA GAAACTGGAG CAGGATCTCG ACACTATTCG GAAATCTGCA 
CCGCATAAAT TGCCTATAAT AAGACTGAAA CGTTGTAATC TCAAGACCCA GAAACGGTCA 
CTCAAAGCTG AAGAAGCACC CAGTGTCCAA GAGGATATAC TTTCTATGAA CTCTGATCGA 
GAATTACAGG AACAAAAAAA AGCGCAGAAG CCTAAAAGAA AGCGATACAA AAAAGTGCAA 
AAGGAGCAAG AAAACGAAAA ATATTTGCGG GAACAAGAAA TTATAATATC GCAAAGCCAA 
ATGGTTGAGG AAGATAAAGT GGAGGAACAC CACGCTCACG AAGCGCCAAG TGAGCAGCAA 
CGAGAGCTCG ATCTGGAGCA TCAACAATTT CTGGAAGAGC AGCTGCTACA GGAGGAACAT 
CAATTGGCGA TGCACGAGGA AGAGCATCAC CAAATGGATG ATGAACAAGA GTCACACGAA 
GTAGCTCGTC TGCTAGAAGA TCAGCAGCCG GCGGAGGGAA CACATCTTCA TGTTGAGAAT 
GAAATGTTGG CAGAGAAAGA GCAACCTCTA ATAGAAGATG ATGACCAGGA AGCGGATGAT 
CAAAATCAAA ATGAGCACTT ATCGGATGAT CAGCAACAGG AAGCGGATCA ACAGCTGTCT 
ATAGAGCAGC CTCAGGAGCA CGAAGAGAAT CAGTCACTCA GCCAAGATCC GTTAGACGAA 
CAATGTACGC CCACACCGAA CGGCAAAAGG CGCAAGCGAA GACGGCGATT CAAGCGACGT 
TGTTGGACGC ACGTAAAGAA TAAGAAAAAG AAGCAGTGGC TAATAGATTA CCCATTGGAG 
TTTGAGGCAG ATCCCGCGTT AGATACCCCT CCTATTCAAC CAGAGCCAGC TCCAGATGAA 
CAACAAGCCG AGCTAGTTAC CGGGCCAGTT TCCGAGTCAG TTGCTGAGCC AGTTGCTGAG 
CCAGTTGCCG AGCCAGTTTC TGAGCCATTT GCAGAGCAAA TTGCCGAGCC AATTGCCCAG 
CCGACACCAG AACCCGCCTA CGATTTCGAT CAGGACTTGG TGCCCGAGGT GGGCAATGAA 
TTGGAGATTG CTGCTCCCAT TCGCGTCGCC GAGGACGAAG ACAGCACTGC GATACTCTCG 
CCGTCCCACA ACCTGGAACT GGTGACCAGC CACGTGCCGG TTTACTTGCT CAATGGAGAA 
AGTCGCGATG TCAGCACACC CCTGTCCAGC AGCTCTTACA GTGCCGGGGG GTCAGAGATA 
TATGCGTCGT CGAAGGATTT AGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG12316 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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