CG13457 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG13457
Entrez Gene ID	39649
Full Name	CG13457 gene product from transcript CG13457-RA
Synonyms	BcDNA:AT15860,BcDNA:AT16542,Dmel\CG13457
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 71B5-71B5

mRNA and Protein(s)

mRNA	Protein	Name
NM_140494.2	NP_648751.1	uncharacterized protein, isoform B
NM_168610.2	NP_730028.1	uncharacterized protein, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG13457 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG13457 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa20236	NM_168610.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG13457), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa20237	NM_140494.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG13457), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG13457 ( NM_168610.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730028.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG13457), mRNA.

Price & Availability↑

CloneID	OFa20236
Clone ID Related Accession (Same CDS sequence)	NM_168610.2
Accession Version	NM_168610.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 31, 2011 this sequence version replaced NM_168610.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCCGAT CTCCGCGTCC AGAGGAGGCG AAGGAAAAAA ACATGACTTG GAGCCAGAGC 
TTGAAGGCGA ATCAAGCGAA AGAGAGTCCT CTGTCCGTCA ATAGGAGTCC TGCATCCGGT 
TCTCAGCAGA AAGTTAAGTG GGCCAAGTGC CGGACCATCG CAATCGCAGT CACGGATAAT 
TCATCAAACG CCAAAAGAGC CAGGGGAGGT TACTGTTGTA ATTGTGATTA TTGTACTCAA 
GACATCCGAG GTGTGTACCA AAACAGGTAT GGAACTACTC CCTGCTGCAA CTGCAAGGGC 
AGCTGGGACA TGGGGTATCA TCAACAGTGC TGTGCTTACG GTGATCTCTG TGGAGAGACC 
CCATTACCCC ATCAGGGTAC ACAATTTGGA ATATCTTATA ACATGTATCC GCGTCCAACT 
TGCAGAAGAT TAGTGACCGA CCTAAATACA GAGGTATTTT GCCGCCACTG GCGTCCGAAA 
ATCGAACGTT CTTTATCACC AAAGGAGCGA AAGGGTTGTG GACCTCAAGT TAAATGCCAG 
GAGAAAAGAA ATCCCTCTAA AATTGGAAAG TGCATTAATA ATAAAAAACT AGAATGTAAT 
GTTAAGAGGG AGGCTACTTC AATAGTTCAG AATAAATGTA GAAAACCTGA GAAAGCGATA 
AAAATTCAAC AGAAGAAGAG GCACGAGTCT ACCAAGGATC ATGGACAAAA GAAATGCAAG 
CCGATAGACA GCCATTTTGC GTCTTTGAAA GGAATGCCCT GTAAAGCTCA ACCATCCAAG 
GTTTGCACAG ATGAACTTAA GGATGAAATC AATTCCCAAA GTTATCAAGA TTATTTCAAT 
CTGTATAATC AACAAAATTG CAAGCCTCTG GATCCGTATC CGTATTCGAC ATACACAAGA 
ATGGCGGGTA AGGATGAAAC GAAATTACCA AAACTTAAGG ATACTAGTTA TTCAGATAGG 
ATAACGGACC ATTATACGGA GAACTCATAT TCATTTGAAG ATAAAACTGA ATATGATTGC 
TTATGCTGCA AAGAAAGAAC TGCTCACTAT CAATTGGAAG ACATTGGATC TGGCGCTTCA 
ACTCCAGATG GTATTCCATA TCAGTGTAGT GAAGAAAAAG ATTCTTATAC CAATGGTTAT 
GCTTGTTCTG AGGAAAGGCG GTACATCACT CCAGATGCAA GCCTACGAGC TAAGTCCCAT 
ATCGAAAGAA ACTTTCATAG AAGCGGTGAA GTCACAAATG CAAATCGCAC GCATGCCCAG 
CAATCCAAAT CCTTGATGGC GAAGGACAAT AATTGTAAAG CAGTAAGGCG GCAAAGGCGG 
GAGCACTGCG AGGAGCGTGT GCAGCCAGAT TCGAAGTGTG GTGATAGGCG AATAAGAGCT 
CATTCAGTTC AGCGATCAGT TTTAGCCCAA AGCAGAGGAC CCACCAGCTA TACTTCGATA 
GATCCTCAGC CTCCTTCAGC CAAGCATGAA ACAAAGGACA CCCAGACGGA ATGCTTGTGG 
CGGGAGAGGC CTACGCAAAC AGAGCGGCAG ATCAGGATGG ATAAAGGCTG TCAAGTGTGC 
CGAGAACGCA ACCAGTTTCG GGATCGGACC AATCCTGCAT CAGCAATTCC ATCTCATTCC 
AAAAACTTTC CCTATAATTC AATGGCAACC ATGCAAAGTG AATGTGCTGA GGCTGTGATG 
AAAGGCACCT ATTTCGATGA AGAATATTGT GCTCCTGTCA TATCCAAACT CGAATCTTCG 
AGCAATAGTA AGCCACTTGA ACAATTTTCC AGAAAAAGAA CAGATTACGT TCATGTCCAT 
GAAGAGGGAC ACCCTGGGCT ATTGGATTGC GGACAAGATA GTCCAAGAAC ACCAAGAGAA 
ATTCAGCACA TTACCTATAA GCAGTCTGTT TCGCAGCAGG TCCAGACTCC TCCATATAGT 
CCACGGACAC CACTGCTATC TTATAGACAG CATAAAAGTT CTAAAAACGA GTATATTACC 
CACACTCCAC AAGCTGGCAC AAATTCCCGG AGCCAAAGTC CATATAGAAG TAGTCCTGTA 
GAAGATCTAT CGTCGAGTTC TCATAAAAGT TCTGGAAAAG TTTATGATAC TCAGACCCGT 
CAAGTCGTCG CAAATTCCCG AAGCAACAGT CCATATAGAA ATAATCCAGC TGAAGATCTA 
TCGTTAAGTC CTCAATTACA ACGCTCTCTG TGTCAGTACA TTCAAAATAT GCCCGGACAT 
ATTGATGACG CGTCTTTCCA AGTGGATTCC CCCAGCTGCA CCAATGACAT ACCCTCGAGA 
TGTGTGGTGG AGACTCGACC TGAATGTGTT CATTGTGAAG GAGAGCATTG TGAACAGTCC 
TGTAAGCAGC ATAGCCCCAG ATCGTGTATG TCTTCTTCCC CAAGACAAAT TTCAGTTTTG 
GAGACCGTCT GTGAAAAGCG CACGCGAACA GTGACCTTCG AGGACGAGAA AGGGGGCCAG 
ACCAAGGAGG AGCATACGAA GGAAAGTTGT AGCAGTCGCA TCGATTGGGA GCGCGCCCTC 
AAATATCACA CATCAGATCA CGGCGATGAG ACGGATTATA CGGGACCTAG CTCCACTTGC 
AGATCCAGCA GCTCTGCGGA GCACACGTGC AATGAGTCCA CCTACGATGA TGACTTCGCC 
AGCTGCGATG AGTCGCAACC GCAGACGATT CACATGCGAA TACCTCCGTT TAATGGTTGT 
CCCTGCATGT ACCAGACCTA CTTGAATCTG GCCACTATGT GCCAGAACGA AGGTGGTGGC 
TTCATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730028.1
CDS	386..3154
Translation	MSRSPRPEEAKEKNMTWSQSLKANQAKESPLSVNRSPASGSQQKVKWAKCRTIAIAVTDNSSNAKRARGGYCCNCDYCTQDIRGVYQNRYGTTPCCNCKGSWDMGYHQQCCAYGDLCGETPLPHQGTQFGISYNMYPRPTCRRLVTDLNTEVFCRHWRPKIERSLSPKERKGCGPQVKCQEKRNPSKIGKCINNKKLECNVKREATSIVQNKCRKPEKAIKIQQKKRHESTKDHGQKKCKPIDSHFASLKGMPCKAQPSKVCTDELKDEINSQSYQDYFNLYNQQNCKPLDPYPYSTYTRMAGKDETKLPKLKDTSYSDRITDHYTENSYSFEDKTEYDCLCCKERTAHYQLEDIGSGASTPDGIPYQCSEEKDSYTNGYACSEERRYITPDASLRAKSHIERNFHRSGEVTNANRTHAQQSKSLMAKDNNCKAVRRQRREHCEERVQPDSKCGDRRIRAHSVQRSVLAQSRGPTSYTSIDPQPPSAKHETKDTQTECLWRERPTQTERQIRMDKGCQVCRERNQFRDRTNPASAIPSHSKNFPYNSMATMQSECAEAVMKGTYFDEEYCAPVISKLESSSNSKPLEQFSRKRTDYVHVHEEGHPGLLDCGQDSPRTPREIQHITYKQSVSQQVQTPPYSPRTPLLSYRQHKSSKNEYITHTPQAGTNSRSQSPYRSSPVEDLSSSSHKSSGKVYDTQTRQVVANSRSNSPYRNNPAEDLSLSPQLQRSLCQYIQNMPGHIDDASFQVDSPSCTNDIPSRCVVETRPECVHCEGEHCEQSCKQHSPRSCMSSSPRQISVLETVCEKRTRTVTFEDEKGGQTKEEHTKESCSSRIDWERALKYHTSDHGDETDYTGPSSTCRSSSSAEHTCNESTYDDDFASCDESQPQTIHMRIPPFNGCPCMYQTYLNLATMCQNEGGGFI

Target ORF information:

RefSeq Version	NM_168610.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG13457), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168610.2

ORF Insert Sequence:

ATGAGCCGAT CTCCGCGTCC AGAGGAGGCG AAGGAAAAAA ACATGACTTG GAGCCAGAGC 
TTGAAGGCGA ATCAAGCGAA AGAGAGTCCT CTGTCCGTCA ATAGGAGTCC TGCATCCGGT 
TCTCAGCAGA AAGTTAAGTG GGCCAAGTGC CGGACCATCG CAATCGCAGT CACGGATAAT 
TCATCAAACG CCAAAAGAGC CAGGGGAGGT TACTGTTGTA ATTGTGATTA TTGTACTCAA 
GACATCCGAG GTGTGTACCA AAACAGGTAT GGAACTACTC CCTGCTGCAA CTGCAAGGGC 
AGCTGGGACA TGGGGTATCA TCAACAGTGC TGTGCTTACG GTGATCTCTG TGGAGAGACC 
CCATTACCCC ATCAGGGTAC ACAATTTGGA ATATCTTATA ACATGTATCC GCGTCCAACT 
TGCAGAAGAT TAGTGACCGA CCTAAATACA GAGGTATTTT GCCGCCACTG GCGTCCGAAA 
ATCGAACGTT CTTTATCACC AAAGGAGCGA AAGGGTTGTG GACCTCAAGT TAAATGCCAG 
GAGAAAAGAA ATCCCTCTAA AATTGGAAAG TGCATTAATA ATAAAAAACT AGAATGTAAT 
GTTAAGAGGG AGGCTACTTC AATAGTTCAG AATAAATGTA GAAAACCTGA GAAAGCGATA 
AAAATTCAAC AGAAGAAGAG GCACGAGTCT ACCAAGGATC ATGGACAAAA GAAATGCAAG 
CCGATAGACA GCCATTTTGC GTCTTTGAAA GGAATGCCCT GTAAAGCTCA ACCATCCAAG 
GTTTGCACAG ATGAACTTAA GGATGAAATC AATTCCCAAA GTTATCAAGA TTATTTCAAT 
CTGTATAATC AACAAAATTG CAAGCCTCTG GATCCGTATC CGTATTCGAC ATACACAAGA 
ATGGCGGGTA AGGATGAAAC GAAATTACCA AAACTTAAGG ATACTAGTTA TTCAGATAGG 
ATAACGGACC ATTATACGGA GAACTCATAT TCATTTGAAG ATAAAACTGA ATATGATTGC 
TTATGCTGCA AAGAAAGAAC TGCTCACTAT CAATTGGAAG ACATTGGATC TGGCGCTTCA 
ACTCCAGATG GTATTCCATA TCAGTGTAGT GAAGAAAAAG ATTCTTATAC CAATGGTTAT 
GCTTGTTCTG AGGAAAGGCG GTACATCACT CCAGATGCAA GCCTACGAGC TAAGTCCCAT 
ATCGAAAGAA ACTTTCATAG AAGCGGTGAA GTCACAAATG CAAATCGCAC GCATGCCCAG 
CAATCCAAAT CCTTGATGGC GAAGGACAAT AATTGTAAAG CAGTAAGGCG GCAAAGGCGG 
GAGCACTGCG AGGAGCGTGT GCAGCCAGAT TCGAAGTGTG GTGATAGGCG AATAAGAGCT 
CATTCAGTTC AGCGATCAGT TTTAGCCCAA AGCAGAGGAC CCACCAGCTA TACTTCGATA 
GATCCTCAGC CTCCTTCAGC CAAGCATGAA ACAAAGGACA CCCAGACGGA ATGCTTGTGG 
CGGGAGAGGC CTACGCAAAC AGAGCGGCAG ATCAGGATGG ATAAAGGCTG TCAAGTGTGC 
CGAGAACGCA ACCAGTTTCG GGATCGGACC AATCCTGCAT CAGCAATTCC ATCTCATTCC 
AAAAACTTTC CCTATAATTC AATGGCAACC ATGCAAAGTG AATGTGCTGA GGCTGTGATG 
AAAGGCACCT ATTTCGATGA AGAATATTGT GCTCCTGTCA TATCCAAACT CGAATCTTCG 
AGCAATAGTA AGCCACTTGA ACAATTTTCC AGAAAAAGAA CAGATTACGT TCATGTCCAT 
GAAGAGGGAC ACCCTGGGCT ATTGGATTGC GGACAAGATA GTCCAAGAAC ACCAAGAGAA 
ATTCAGCACA TTACCTATAA GCAGTCTGTT TCGCAGCAGG TCCAGACTCC TCCATATAGT 
CCACGGACAC CACTGCTATC TTATAGACAG CATAAAAGTT CTAAAAACGA GTATATTACC 
CACACTCCAC AAGCTGGCAC AAATTCCCGG AGCCAAAGTC CATATAGAAG TAGTCCTGTA 
GAAGATCTAT CGTCGAGTTC TCATAAAAGT TCTGGAAAAG TTTATGATAC TCAGACCCGT 
CAAGTCGTCG CAAATTCCCG AAGCAACAGT CCATATAGAA ATAATCCAGC TGAAGATCTA 
TCGTTAAGTC CTCAATTACA ACGCTCTCTG TGTCAGTACA TTCAAAATAT GCCCGGACAT 
ATTGATGACG CGTCTTTCCA AGTGGATTCC CCCAGCTGCA CCAATGACAT ACCCTCGAGA 
TGTGTGGTGG AGACTCGACC TGAATGTGTT CATTGTGAAG GAGAGCATTG TGAACAGTCC 
TGTAAGCAGC ATAGCCCCAG ATCGTGTATG TCTTCTTCCC CAAGACAAAT TTCAGTTTTG 
GAGACCGTCT GTGAAAAGCG CACGCGAACA GTGACCTTCG AGGACGAGAA AGGGGGCCAG 
ACCAAGGAGG AGCATACGAA GGAAAGTTGT AGCAGTCGCA TCGATTGGGA GCGCGCCCTC 
AAATATCACA CATCAGATCA CGGCGATGAG ACGGATTATA CGGGACCTAG CTCCACTTGC 
AGATCCAGCA GCTCTGCGGA GCACACGTGC AATGAGTCCA CCTACGATGA TGACTTCGCC 
AGCTGCGATG AGTCGCAACC GCAGACGATT CACATGCGAA TACCTCCGTT TAATGGTTGT 
CCCTGCATGT ACCAGACCTA CTTGAATCTG GCCACTATGT GCCAGAACGA AGGTGGTGGC 
TTCATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG13457 ( NM_140494.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648751.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG13457), mRNA.

Price & Availability↑

CloneID	OFa20237
Clone ID Related Accession (Same CDS sequence)	NM_140494.2
Accession Version	NM_140494.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 31, 2011 this sequence version replaced NM_140494.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAACA AGACCGGTGA ATGTTCCAGT TACTTACTGA AACGACAGGA GCGAGTCTCG 
GTCGAGGAAC ACCAACGGAT CTACATAAAG AACACTCAGG TAACGCAGGT AAGGGAACTC 
TTCCGCCGTC CGGTGGTTAG AGTTTCCGGT TCCACCTTGA TAAAACCAAA AGCGACGGGT 
TCATTTTCCT CTGGGAAAAT CCAACAAACT CTCCAGAAGG TGGAAGTCCT AAACGTGAGG 
ATCGGCAATG AGTTAAATAG GTTAGAGCGC TTTCGTAATC ATTTTCTGGA AGTCAAAGAA 
GCTATGAGCC GATCTCCGCG TCCAGAGGAG GCGAAGGAAA AAAACATGAC TTGGAGCCAG 
AGCTTGAAGG CGAATCAAGC GAAAGAGAGT CCTCTGTCCG TCAATAGGAG TCCTGCATCC 
GGTTCTCAGC AGAAAGTTAA GTGGGCCAAG TGCCGGACCA TCGCAATCGC AGTCACGGAT 
AATTCATCAA ACGCCAAAAG AGCCAGGGGA GGTTACTGTT GTAATTGTGA TTATTGTACT 
CAAGACATCC GAGGTGTGTA CCAAAACAGG TATGGAACTA CTCCCTGCTG CAACTGCAAG 
GGCAGCTGGG ACATGGGGTA TCATCAACAG TGCTGTGCTT ACGGTGATCT CTGTGGAGAG 
ACCCCATTAC CCCATCAGGG TACACAATTT GGAATATCTT ATAACATGTA TCCGCGTCCA 
ACTTGCAGAA GATTAGTGAC CGACCTAAAT ACAGAGGTAT TTTGCCGCCA CTGGCGTCCG 
AAAATCGAAC GTTCTTTATC ACCAAAGGAG CGAAAGGGTT GTGGACCTCA AGTTAAATGC 
CAGGAGAAAA GAAATCCCTC TAAAATTGGA AAGTGCATTA ATAATAAAAA ACTAGAATGT 
AATGTTAAGA GGGAGGCTAC TTCAATAGTT CAGAATAAAT GTAGAAAACC TGAGAAAGCG 
ATAAAAATTC AACAGAAGAA GAGGCACGAG TCTACCAAGG ATCATGGACA AAAGAAATGC 
AAGCCGATAG ACAGCCATTT TGCGTCTTTG AAAGGAATGC CCTGTAAAGC TCAACCATCC 
AAGGTTTGCA CAGATGAACT TAAGGATGAA ATCAATTCCC AAAGTTATCA AGATTATTTC 
AATCTGTATA ATCAACAAAA TTGCAAGCCT CTGGATCCGT ATCCGTATTC GACATACACA 
AGAATGGCGG GTAAGGATGA AACGAAATTA CCAAAACTTA AGGATACTAG TTATTCAGAT 
AGGATAACGG ACCATTATAC GGAGAACTCA TATTCATTTG AAGATAAAAC TGAATATGAT 
TGCTTATGCT GCAAAGAAAG AACTGCTCAC TATCAATTGG AAGACATTGG ATCTGGCGCT 
TCAACTCCAG ATGGTATTCC ATATCAGTGT AGTGAAGAAA AAGATTCTTA TACCAATGGT 
TATGCTTGTT CTGAGGAAAG GCGGTACATC ACTCCAGATG CAAGCCTACG AGCTAAGTCC 
CATATCGAAA GAAACTTTCA TAGAAGCGGT GAAGTCACAA ATGCAAATCG CACGCATGCC 
CAGCAATCCA AATCCTTGAT GGCGAAGGAC AATAATTGTA AAGCAGTAAG GCGGCAAAGG 
CGGGAGCACT GCGAGGAGCG TGTGCAGCCA GATTCGAAGT GTGGTGATAG GCGAATAAGA 
GCTCATTCAG TTCAGCGATC AGTTTTAGCC CAAAGCAGAG GACCCACCAG CTATACTTCG 
ATAGATCCTC AGCCTCCTTC AGCCAAGCAT GAAACAAAGG ACACCCAGAC GGAATGCTTG 
TGGCGGGAGA GGCCTACGCA AACAGAGCGG CAGATCAGGA TGGATAAAGG CTGTCAAGTG 
TGCCGAGAAC GCAACCAGTT TCGGGATCGG ACCAATCCTG CATCAGCAAT TCCATCTCAT 
TCCAAAAACT TTCCCTATAA TTCAATGGCA ACCATGCAAA GTGAATGTGC TGAGGCTGTG 
ATGAAAGGCA CCTATTTCGA TGAAGAATAT TGTGCTCCTG TCATATCCAA ACTCGAATCT 
TCGAGCAATA GTAAGCCACT TGAACAATTT TCCAGAAAAA GAACAGATTA CGTTCATGTC 
CATGAAGAGG GACACCCTGG GCTATTGGAT TGCGGACAAG ATAGTCCAAG AACACCAAGA 
GAAATTCAGC ACATTACCTA TAAGCAGTCT GTTTCGCAGC AGGTCCAGAC TCCTCCATAT 
AGTCCACGGA CACCACTGCT ATCTTATAGA CAGCATAAAA GTTCTAAAAA CGAGTATATT 
ACCCACACTC CACAAGCTGG CACAAATTCC CGGAGCCAAA GTCCATATAG AAGTAGTCCT 
GTAGAAGATC TATCGTCGAG TTCTCATAAA AGTTCTGGAA AAGTTTATGA TACTCAGACC 
CGTCAAGTCG TCGCAAATTC CCGAAGCAAC AGTCCATATA GAAATAATCC AGCTGAAGAT 
CTATCGTTAA GTCCTCAATT ACAACGCTCT CTGTGTCAGT ACATTCAAAA TATGCCCGGA 
CATATTGATG ACGCGTCTTT CCAAGTGGAT TCCCCCAGCT GCACCAATGA CATACCCTCG 
AGATGTGTGG TGGAGACTCG ACCTGAATGT GTTCATTGTG AAGGAGAGCA TTGTGAACAG 
TCCTGTAAGC AGCATAGCCC CAGATCGTGT ATGTCTTCTT CCCCAAGACA AATTTCAGTT 
TTGGAGACCG TCTGTGAAAA GCGCACGCGA ACAGTGACCT TCGAGGACGA GAAAGGGGGC 
CAGACCAAGG AGGAGCATAC GAAGGAAAGT TGTAGCAGTC GCATCGATTG GGAGCGCGCC 
CTCAAATATC ACACATCAGA TCACGGCGAT GAGACGGATT ATACGGGACC TAGCTCCACT 
TGCAGATCCA GCAGCTCTGC GGAGCACACG TGCAATGAGT CCACCTACGA TGATGACTTC 
GCCAGCTGCG ATGAGTCGCA ACCGCAGACG ATTCACATGC GAATACCTCC GTTTAATGGT 
TGTCCCTGCA TGTACCAGAC CTACTTGAAT CTGGCCACTA TGTGCCAGAA CGAAGGTGGT 
GGCTTCATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648751.1
CDS	90..3161
Translation	MENKTGECSSYLLKRQERVSVEEHQRIYIKNTQVTQVRELFRRPVVRVSGSTLIKPKATGSFSSGKIQQTLQKVEVLNVRIGNELNRLERFRNHFLEVKEAMSRSPRPEEAKEKNMTWSQSLKANQAKESPLSVNRSPASGSQQKVKWAKCRTIAIAVTDNSSNAKRARGGYCCNCDYCTQDIRGVYQNRYGTTPCCNCKGSWDMGYHQQCCAYGDLCGETPLPHQGTQFGISYNMYPRPTCRRLVTDLNTEVFCRHWRPKIERSLSPKERKGCGPQVKCQEKRNPSKIGKCINNKKLECNVKREATSIVQNKCRKPEKAIKIQQKKRHESTKDHGQKKCKPIDSHFASLKGMPCKAQPSKVCTDELKDEINSQSYQDYFNLYNQQNCKPLDPYPYSTYTRMAGKDETKLPKLKDTSYSDRITDHYTENSYSFEDKTEYDCLCCKERTAHYQLEDIGSGASTPDGIPYQCSEEKDSYTNGYACSEERRYITPDASLRAKSHIERNFHRSGEVTNANRTHAQQSKSLMAKDNNCKAVRRQRREHCEERVQPDSKCGDRRIRAHSVQRSVLAQSRGPTSYTSIDPQPPSAKHETKDTQTECLWRERPTQTERQIRMDKGCQVCRERNQFRDRTNPASAIPSHSKNFPYNSMATMQSECAEAVMKGTYFDEEYCAPVISKLESSSNSKPLEQFSRKRTDYVHVHEEGHPGLLDCGQDSPRTPREIQHITYKQSVSQQVQTPPYSPRTPLLSYRQHKSSKNEYITHTPQAGTNSRSQSPYRSSPVEDLSSSSHKSSGKVYDTQTRQVVANSRSNSPYRNNPAEDLSLSPQLQRSLCQYIQNMPGHIDDASFQVDSPSCTNDIPSRCVVETRPECVHCEGEHCEQSCKQHSPRSCMSSSPRQISVLETVCEKRTRTVTFEDEKGGQTKEEHTKESCSSRIDWERALKYHTSDHGDETDYTGPSSTCRSSSSAEHTCNESTYDDDFASCDESQPQTIHMRIPPFNGCPCMYQTYLNLATMCQNEGGGFI

Target ORF information:

RefSeq Version	NM_140494.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG13457), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140494.2

ORF Insert Sequence:

ATGGAGAACA AGACCGGTGA ATGTTCCAGT TACTTACTGA AACGACAGGA GCGAGTCTCG 
GTCGAGGAAC ACCAACGGAT CTACATAAAG AACACTCAGG TAACGCAGGT AAGGGAACTC 
TTCCGCCGTC CGGTGGTTAG AGTTTCCGGT TCCACCTTGA TAAAACCAAA AGCGACGGGT 
TCATTTTCCT CTGGGAAAAT CCAACAAACT CTCCAGAAGG TGGAAGTCCT AAACGTGAGG 
ATCGGCAATG AGTTAAATAG GTTAGAGCGC TTTCGTAATC ATTTTCTGGA AGTCAAAGAA 
GCTATGAGCC GATCTCCGCG TCCAGAGGAG GCGAAGGAAA AAAACATGAC TTGGAGCCAG 
AGCTTGAAGG CGAATCAAGC GAAAGAGAGT CCTCTGTCCG TCAATAGGAG TCCTGCATCC 
GGTTCTCAGC AGAAAGTTAA GTGGGCCAAG TGCCGGACCA TCGCAATCGC AGTCACGGAT 
AATTCATCAA ACGCCAAAAG AGCCAGGGGA GGTTACTGTT GTAATTGTGA TTATTGTACT 
CAAGACATCC GAGGTGTGTA CCAAAACAGG TATGGAACTA CTCCCTGCTG CAACTGCAAG 
GGCAGCTGGG ACATGGGGTA TCATCAACAG TGCTGTGCTT ACGGTGATCT CTGTGGAGAG 
ACCCCATTAC CCCATCAGGG TACACAATTT GGAATATCTT ATAACATGTA TCCGCGTCCA 
ACTTGCAGAA GATTAGTGAC CGACCTAAAT ACAGAGGTAT TTTGCCGCCA CTGGCGTCCG 
AAAATCGAAC GTTCTTTATC ACCAAAGGAG CGAAAGGGTT GTGGACCTCA AGTTAAATGC 
CAGGAGAAAA GAAATCCCTC TAAAATTGGA AAGTGCATTA ATAATAAAAA ACTAGAATGT 
AATGTTAAGA GGGAGGCTAC TTCAATAGTT CAGAATAAAT GTAGAAAACC TGAGAAAGCG 
ATAAAAATTC AACAGAAGAA GAGGCACGAG TCTACCAAGG ATCATGGACA AAAGAAATGC 
AAGCCGATAG ACAGCCATTT TGCGTCTTTG AAAGGAATGC CCTGTAAAGC TCAACCATCC 
AAGGTTTGCA CAGATGAACT TAAGGATGAA ATCAATTCCC AAAGTTATCA AGATTATTTC 
AATCTGTATA ATCAACAAAA TTGCAAGCCT CTGGATCCGT ATCCGTATTC GACATACACA 
AGAATGGCGG GTAAGGATGA AACGAAATTA CCAAAACTTA AGGATACTAG TTATTCAGAT 
AGGATAACGG ACCATTATAC GGAGAACTCA TATTCATTTG AAGATAAAAC TGAATATGAT 
TGCTTATGCT GCAAAGAAAG AACTGCTCAC TATCAATTGG AAGACATTGG ATCTGGCGCT 
TCAACTCCAG ATGGTATTCC ATATCAGTGT AGTGAAGAAA AAGATTCTTA TACCAATGGT 
TATGCTTGTT CTGAGGAAAG GCGGTACATC ACTCCAGATG CAAGCCTACG AGCTAAGTCC 
CATATCGAAA GAAACTTTCA TAGAAGCGGT GAAGTCACAA ATGCAAATCG CACGCATGCC 
CAGCAATCCA AATCCTTGAT GGCGAAGGAC AATAATTGTA AAGCAGTAAG GCGGCAAAGG 
CGGGAGCACT GCGAGGAGCG TGTGCAGCCA GATTCGAAGT GTGGTGATAG GCGAATAAGA 
GCTCATTCAG TTCAGCGATC AGTTTTAGCC CAAAGCAGAG GACCCACCAG CTATACTTCG 
ATAGATCCTC AGCCTCCTTC AGCCAAGCAT GAAACAAAGG ACACCCAGAC GGAATGCTTG 
TGGCGGGAGA GGCCTACGCA AACAGAGCGG CAGATCAGGA TGGATAAAGG CTGTCAAGTG 
TGCCGAGAAC GCAACCAGTT TCGGGATCGG ACCAATCCTG CATCAGCAAT TCCATCTCAT 
TCCAAAAACT TTCCCTATAA TTCAATGGCA ACCATGCAAA GTGAATGTGC TGAGGCTGTG 
ATGAAAGGCA CCTATTTCGA TGAAGAATAT TGTGCTCCTG TCATATCCAA ACTCGAATCT 
TCGAGCAATA GTAAGCCACT TGAACAATTT TCCAGAAAAA GAACAGATTA CGTTCATGTC 
CATGAAGAGG GACACCCTGG GCTATTGGAT TGCGGACAAG ATAGTCCAAG AACACCAAGA 
GAAATTCAGC ACATTACCTA TAAGCAGTCT GTTTCGCAGC AGGTCCAGAC TCCTCCATAT 
AGTCCACGGA CACCACTGCT ATCTTATAGA CAGCATAAAA GTTCTAAAAA CGAGTATATT 
ACCCACACTC CACAAGCTGG CACAAATTCC CGGAGCCAAA GTCCATATAG AAGTAGTCCT 
GTAGAAGATC TATCGTCGAG TTCTCATAAA AGTTCTGGAA AAGTTTATGA TACTCAGACC 
CGTCAAGTCG TCGCAAATTC CCGAAGCAAC AGTCCATATA GAAATAATCC AGCTGAAGAT 
CTATCGTTAA GTCCTCAATT ACAACGCTCT CTGTGTCAGT ACATTCAAAA TATGCCCGGA 
CATATTGATG ACGCGTCTTT CCAAGTGGAT TCCCCCAGCT GCACCAATGA CATACCCTCG 
AGATGTGTGG TGGAGACTCG ACCTGAATGT GTTCATTGTG AAGGAGAGCA TTGTGAACAG 
TCCTGTAAGC AGCATAGCCC CAGATCGTGT ATGTCTTCTT CCCCAAGACA AATTTCAGTT 
TTGGAGACCG TCTGTGAAAA GCGCACGCGA ACAGTGACCT TCGAGGACGA GAAAGGGGGC 
CAGACCAAGG AGGAGCATAC GAAGGAAAGT TGTAGCAGTC GCATCGATTG GGAGCGCGCC 
CTCAAATATC ACACATCAGA TCACGGCGAT GAGACGGATT ATACGGGACC TAGCTCCACT 
TGCAGATCCA GCAGCTCTGC GGAGCACACG TGCAATGAGT CCACCTACGA TGATGACTTC 
GCCAGCTGCG ATGAGTCGCA ACCGCAGACG ATTCACATGC GAATACCTCC GTTTAATGGT 
TGTCCCTGCA TGTACCAGAC CTACTTGAAT CTGGCCACTA TGTGCCAGAA CGAAGGTGGT 
GGCTTCATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG13457 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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