Cep135 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cep135
Entrez Gene ID	39647
Full Name	Centrosomal protein 135kDa
Synonyms	Bld-10,Bld10,Bld10p,CEP135,CG17081,DCep135,DCep135/Bld10,DmBLD10,Dmel\CG17081,bld10,dbld10
General protein information	Preferred Names Centrosomal protein 135kDa Names CG17081 gene product from transcript CG17081-RB CG17081-PA CG17081-PB CG17081-PC Cep135-PA Cep135-PB Cep135-PC centrosomal protein 135kDa
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 71B5-71B5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274940.1	NP_001261869.1	centrosomal protein 135kDa, isoform C
NM_140492.4	NP_648749.3	centrosomal protein 135kDa, isoform A
NM_001202179.2	NP_001189108.1	centrosomal protein 135kDa, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Cep135	NP_001189108.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010985	XP_309709.4
Bos taurus (cattle)	CEP135	NP_001068874.2
Xenopus tropicalis (tropical clawed frog)	cep135	XP_002934717.1
Homo sapiens (human)	CEP135	NP_079285.2
	CEP135	XP_001084949.2
Rattus norvegicus (Norway rat)	Cep135	XP_223341.6
Gallus gallus (chicken)	CEP135	XP_004936077.1
Danio rerio (zebrafish)	cep135	NP_001186919.1
Pan troglodytes (chimpanzee)	CEP135	XP_517281.2
Canis lupus familiaris (dog)	CEP135	XP_539276.3
Mus musculus (house mouse)	Cep135	NP_950197.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ana1 mutants lack detectable centrosomes in vivo, that Ana1 is irreversibly incorporated into centrioles during their assembly and appears to play a more important role in maintaining Asl at centrioles than in initially recruiting Asl to centrioles...the N-terminal region of Ana1 (amino acids 1-935) is required to target Ana1 to centrioles through a direct interaction with Cep135, whereas the C-terminal region of Ana1.
Title: Drosophila Ana1 is required for centrosome assembly and centriole elongation.

The centriolar protein Bld10/Cep135 is required to establish centrosome asymmetry in Drosophila neuroblasts.
Title: The centriolar protein Bld10/Cep135 is required to establish centrosome asymmetry in Drosophila neuroblasts.

Cep135/Bld10 is not essential for cartwheel assembly or for establishing the ninefold symmetry of centrioles; rather, it appears to stabilize the connection between inner and outer centriole components.
Title: Drosophila Cep135/Bld10 maintains proper centriole structure but is dispensable for cartwheel formation.

work describes a genetically tractable system to study motile cilia formation and provides an explanation for BLD10/CEP135's role in assembling highly stable microtubule-based structures
Title: BLD10/CEP135 is a microtubule-associated protein that controls the formation of the flagellum central microtubule pair.

Drosophila Bld10 is required for centriole and axoneme assembly to confer cilium motility.
Title: Drosophila bld10 is a centriolar protein that regulates centriole, basal body, and motile cilium assembly.

The following Cep135 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cep135 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18524	NM_001274940.1 Latest version!	Drosophila melanogaster centrosomal protein 135kDa, transcript variant C (Cep135), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa18523	NM_001202179.2 Latest version!	Drosophila melanogaster centrosomal protein 135kDa, transcript variant B (Cep135), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa20234	NM_140492.4 Latest version!	Drosophila melanogaster centrosomal protein 135kDa, transcript variant A (Cep135), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cep135 ( NM_001274940.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261869.1 Drosophila melanogaster centrosomal protein 135kDa, transcript variant C (Cep135), mRNA.

Price & Availability↑

CloneID	OFa18524
Clone ID Related Accession (Same CDS sequence)	NM_001274940.1
Accession Version	NM_001274940.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	centrosomal protein 135kDa, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATATCA ACGATGGTGA CTTTAAAGAC CTCCGCTGCA AACTGGACAT CATGGGTTTC 
ACACAGACGC TGCCCGTTTT GGCCATTCCA CTGGTTCAGG CTGTTTTCGG GGACCTAATC 
AAGACCACAG AATGTTTGAG GGATACCAAG AAAAAGGTGG CCGAGCTGCT GGAGGAAAAG 
ACCTGTTGGG AGCTGGGCGT CGAACCCTAC AAGTGTGACA ACTCTCGCCT GCTGGCCGAG 
TGCAATGAAC TGCATCTCCA GTTTTTGAGG GACAGGGAGG ACTACGAGCT GCGCCTGTGT 
GAGTCCGAGC GAAAGATCCG AAATCTGGAG TTGGACAAGC AGCATTTGCA GAGTCACAAC 
GATGGACTTC AAAGGCAACT GGACGCTTTG CTTACCAAGC AAATGGTTTC ATCAGTCACG 
AACAAAAAGG CGACAGCTGG CATTGGACGT CAGACCAAAA AACCCTTTAT AACCACAGTG 
CGTTCGGGCA TTGCGATGCC AACTACACTG GGCACTAGCA GCAGTGCTCT TAAATGCACA 
AAATGCAATG CTGGGGTATT CCAGAAGACC ACCACAACGA CCAAAGACGG TGTGACAGTG 
ACGCAAACCA GTGGACAGGA GGAGTTAGAC AAGATGCAGA ATGATCTTAC GGCAGCCGGA 
GAACAACTGG AGTTCTTCAA GCGCAAAGTG GAGGCCAGAA ATCGGGAGAT TCGCCGCTTA 
AACGACATGC TAGCAGGAGG GCGTCCTTTA GCGGCTTTGG CCAAGGATTG CTGTTACAAG 
GACGTGGGCG CCCTCTCCCA GGACATCGAT CTGTTGCAAC GCGAGAAGAG CGATTTAATG 
ATGCAAGTTC GAGAATTCCA GGACAAGATG CACGATGCTA TGCAGCGGGC TCTAAGCTCC 
GAAGAGGATA AAATAAAGCT CCAAACGCAG CTGGAGGAGC TCAAGGAAGC TGCGTTGCAA 
GTGGAACAGC AGGCCAATGC GGAAATCGAT GCCAAGGAAT CGGAACTTAG GCAACTGCAG 
TTGGAGCTTA AGAAGAAGGG AAAGGATCAC CGACTGACCG GTGGCTTTGC TTCCAATCAA 
AGTGACAAGC ATAATCTTAA TGAACGCTTA AATCTTTTGA CTCGTCGTGA AGAGGAACTA 
CAGGCCACAA ATGAAAAGCA TAAGAAGAAA ATTCAGAAAA TGCAAGCAAA GATATTAGAA 
CTGCAGAAGG AGCTCAAAGA TCAGAATAAA CATTCCAACG TCACCCTGGA TGAAGAGAAA 
ATACGCCTGT CATCAGAGCG GGACTTCTTC CAGAAGGAGT ATCTGCGTCT GATGAGCAAG 
ACCGGATCCG AGTCGGAGAT CGCCTTCTTG CATGCCCAAA TTAAATCTAA GGACGAGGAG 
TTAAAGGCCC TGCGATCTGA ACTGTTTCAT GGAGGAAAGC AGCAATTTTC CCCACAAAAA 
AGTGTGCAGT ATGAGACACT GCCGCCCCCC ACAGCTTCAT CCATTACCTC CACCGTTACT 
AGCAATACGA GTGACTGCGT CCAGGCGGCC ATTGCAAGGG TGGAGCGGGA ACGGGATTGC 
GCCAGGACCG AGCTAGAAAG GGTTCGTTGC GAGCGGGATA CGCTGAGGGA GAAGCAACTG 
AGCACTGTTC AACTTCATGC CGATGAGCTA CAGGCTCTGC GCCTGCGAAA CGAAGAGCTT 
AACGATCGCC TGCGGCAAAT GGAACGCGAT AATCGGGAAC TTAACTCGGC TCGTCTGCCA 
ACTGAAACGA ATCTGGTGCT GCTTAAGGAG GACTTGTTGC AAATGAGGCA GCGTGTGGCA 
TCTATGCAAA CCGAAATTGA CCAACTAAAG ACCGAAAATG ATCAAATAAC CATGCTCAAC 
GATCAAAACG AGCGCATCAT AGCGGATTAT CAGAGTAAAC TTTTGGTGGC CGAGCGTCAA 
CGACAGTCGG CGGATGTTCG GGCCAGCACC TTGGACTCCA GTCGGGAATC CAATCGCAGC 
GAGGTTACCC AACTCCGCAT GGACTTAGGA GCTCTGCGGC AGACCTATAT ATCGTTAGAA 
CACGAGAAGG ATACGCTTCT GCATCAATTG GATACCAAAA CTGAGCGGGT ATACAAACTG 
GAGTATGAGC TAAAGGATTG CAAAGAAAAA CGGAATGCCC TAGAGCAGAA TGTGAAGGAC 
CTGGAGGATC AATTGCGAAA ACTGGCGAAC AGGAACCGGC AGCGCGACTC AGAACTGACG 
GAGACCTCGA CGGAAAGTAA AACACTGCGC CAGCAGATTG TGGCACTCAA AGCCAGTCGC 
GATGAGGCAA TAGCAGAGAA TCGCCGGCTG ATGGACAAAT TGAGCGATGC CCAAGTGGAG 
GCCAGGACGT TGCAGAAGAA GCTGACGGAT TCCGAGCTTC AGGTGGCCAA CATGAAGCAG 
CAGCTGCACA AGTACGTCCA GGAGGTCAAG AAGGCCGAGG ATTTGCTCAT CCAGAAGGAA 
AAGGAGCGCG ATGATATGCT GGATCAGTAC CACTGTCTAA CCCAGGGCCA AGCTACACTG 
GAGGGTAATA ACCAAAGTCT GGAGTGCGAG GCGGTTGAGT TCAGGCGACA GATCTGTGAG 
CTGGAGTGCG AGGTGCACAG CCTGAAGGAA CAACTGCAGC TTCGCCAGTG CGCACTTCAT 
GATATGGAGG TGCAGCTGAC CGCCGCTCGG GCTTCAGTGC GTTGTTTGGA AAGGGAGCTG 
GAGAATGCCC GAGATGACAT CCGTGTCCAG AAGGTGGATC TTGAGGCGCG CAAGGAGCTG 
TGTGATAAGT TGGATGTGGA AAGAAGCAAA CTCAATGCGG AGCTGAATGA TGTGAATGAA 
ATTCGCAAAA AGGTGAGCTT CAGCTCCGGT CCAGATCACT TTCCGTATCT GTATCCTAGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261869.1
CDS	125..3007
Translation	MNINDGDFKDLRCKLDIMGFTQTLPVLAIPLVQAVFGDLIKTTECLRDTKKKVAELLEEKTCWELGVEPYKCDNSRLLAECNELHLQFLRDREDYELRLCESERKIRNLELDKQHLQSHNDGLQRQLDALLTKQMVSSVTNKKATAGIGRQTKKPFITTVRSGIAMPTTLGTSSSALKCTKCNAGVFQKTTTTTKDGVTVTQTSGQEELDKMQNDLTAAGEQLEFFKRKVEARNREIRRLNDMLAGGRPLAALAKDCCYKDVGALSQDIDLLQREKSDLMMQVREFQDKMHDAMQRALSSEEDKIKLQTQLEELKEAALQVEQQANAEIDAKESELRQLQLELKKKGKDHRLTGGFASNQSDKHNLNERLNLLTRREEELQATNEKHKKKIQKMQAKILELQKELKDQNKHSNVTLDEEKIRLSSERDFFQKEYLRLMSKTGSESEIAFLHAQIKSKDEELKALRSELFHGGKQQFSPQKSVQYETLPPPTASSITSTVTSNTSDCVQAAIARVERERDCARTELERVRCERDTLREKQLSTVQLHADELQALRLRNEELNDRLRQMERDNRELNSARLPTETNLVLLKEDLLQMRQRVASMQTEIDQLKTENDQITMLNDQNERIIADYQSKLLVAERQRQSADVRASTLDSSRESNRSEVTQLRMDLGALRQTYISLEHEKDTLLHQLDTKTERVYKLEYELKDCKEKRNALEQNVKDLEDQLRKLANRNRQRDSELTETSTESKTLRQQIVALKASRDEAIAENRRLMDKLSDAQVEARTLQKKLTDSELQVANMKQQLHKYVQEVKKAEDLLIQKEKERDDMLDQYHCLTQGQATLEGNNQSLECEAVEFRRQICELECEVHSLKEQLQLRQCALHDMEVQLTAARASVRCLERELENARDDIRVQKVDLEARKELCDKLDVERSKLNAELNDVNEIRKKVSFSSGPDHFPYLYPS

Target ORF information:

RefSeq Version	NM_001274940.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster centrosomal protein 135kDa, transcript variant C (Cep135), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274940.1

ORF Insert Sequence:

ATGAATATCA ACGATGGTGA CTTTAAAGAC CTCCGCTGCA AACTGGACAT CATGGGTTTC 
ACACAGACGC TGCCCGTTTT GGCCATTCCA CTGGTTCAGG CTGTTTTCGG GGACCTAATC 
AAGACCACAG AATGTTTGAG GGATACCAAG AAAAAGGTGG CCGAGCTGCT GGAGGAAAAG 
ACCTGTTGGG AGCTGGGCGT CGAACCCTAC AAGTGTGACA ACTCTCGCCT GCTGGCCGAG 
TGCAATGAAC TGCATCTCCA GTTTTTGAGG GACAGGGAGG ACTACGAGCT GCGCCTGTGT 
GAGTCCGAGC GAAAGATCCG AAATCTGGAG TTGGACAAGC AGCATTTGCA GAGTCACAAC 
GATGGACTTC AAAGGCAACT GGACGCTTTG CTTACCAAGC AAATGGTTTC ATCAGTCACG 
AACAAAAAGG CGACAGCTGG CATTGGACGT CAGACCAAAA AACCCTTTAT AACCACAGTG 
CGTTCGGGCA TTGCGATGCC AACTACACTG GGCACTAGCA GCAGTGCTCT TAAATGCACA 
AAATGCAATG CTGGGGTATT CCAGAAGACC ACCACAACGA CCAAAGACGG TGTGACAGTG 
ACGCAAACCA GTGGACAGGA GGAGTTAGAC AAGATGCAGA ATGATCTTAC GGCAGCCGGA 
GAACAACTGG AGTTCTTCAA GCGCAAAGTG GAGGCCAGAA ATCGGGAGAT TCGCCGCTTA 
AACGACATGC TAGCAGGAGG GCGTCCTTTA GCGGCTTTGG CCAAGGATTG CTGTTACAAG 
GACGTGGGCG CCCTCTCCCA GGACATCGAT CTGTTGCAAC GCGAGAAGAG CGATTTAATG 
ATGCAAGTTC GAGAATTCCA GGACAAGATG CACGATGCTA TGCAGCGGGC TCTAAGCTCC 
GAAGAGGATA AAATAAAGCT CCAAACGCAG CTGGAGGAGC TCAAGGAAGC TGCGTTGCAA 
GTGGAACAGC AGGCCAATGC GGAAATCGAT GCCAAGGAAT CGGAACTTAG GCAACTGCAG 
TTGGAGCTTA AGAAGAAGGG AAAGGATCAC CGACTGACCG GTGGCTTTGC TTCCAATCAA 
AGTGACAAGC ATAATCTTAA TGAACGCTTA AATCTTTTGA CTCGTCGTGA AGAGGAACTA 
CAGGCCACAA ATGAAAAGCA TAAGAAGAAA ATTCAGAAAA TGCAAGCAAA GATATTAGAA 
CTGCAGAAGG AGCTCAAAGA TCAGAATAAA CATTCCAACG TCACCCTGGA TGAAGAGAAA 
ATACGCCTGT CATCAGAGCG GGACTTCTTC CAGAAGGAGT ATCTGCGTCT GATGAGCAAG 
ACCGGATCCG AGTCGGAGAT CGCCTTCTTG CATGCCCAAA TTAAATCTAA GGACGAGGAG 
TTAAAGGCCC TGCGATCTGA ACTGTTTCAT GGAGGAAAGC AGCAATTTTC CCCACAAAAA 
AGTGTGCAGT ATGAGACACT GCCGCCCCCC ACAGCTTCAT CCATTACCTC CACCGTTACT 
AGCAATACGA GTGACTGCGT CCAGGCGGCC ATTGCAAGGG TGGAGCGGGA ACGGGATTGC 
GCCAGGACCG AGCTAGAAAG GGTTCGTTGC GAGCGGGATA CGCTGAGGGA GAAGCAACTG 
AGCACTGTTC AACTTCATGC CGATGAGCTA CAGGCTCTGC GCCTGCGAAA CGAAGAGCTT 
AACGATCGCC TGCGGCAAAT GGAACGCGAT AATCGGGAAC TTAACTCGGC TCGTCTGCCA 
ACTGAAACGA ATCTGGTGCT GCTTAAGGAG GACTTGTTGC AAATGAGGCA GCGTGTGGCA 
TCTATGCAAA CCGAAATTGA CCAACTAAAG ACCGAAAATG ATCAAATAAC CATGCTCAAC 
GATCAAAACG AGCGCATCAT AGCGGATTAT CAGAGTAAAC TTTTGGTGGC CGAGCGTCAA 
CGACAGTCGG CGGATGTTCG GGCCAGCACC TTGGACTCCA GTCGGGAATC CAATCGCAGC 
GAGGTTACCC AACTCCGCAT GGACTTAGGA GCTCTGCGGC AGACCTATAT ATCGTTAGAA 
CACGAGAAGG ATACGCTTCT GCATCAATTG GATACCAAAA CTGAGCGGGT ATACAAACTG 
GAGTATGAGC TAAAGGATTG CAAAGAAAAA CGGAATGCCC TAGAGCAGAA TGTGAAGGAC 
CTGGAGGATC AATTGCGAAA ACTGGCGAAC AGGAACCGGC AGCGCGACTC AGAACTGACG 
GAGACCTCGA CGGAAAGTAA AACACTGCGC CAGCAGATTG TGGCACTCAA AGCCAGTCGC 
GATGAGGCAA TAGCAGAGAA TCGCCGGCTG ATGGACAAAT TGAGCGATGC CCAAGTGGAG 
GCCAGGACGT TGCAGAAGAA GCTGACGGAT TCCGAGCTTC AGGTGGCCAA CATGAAGCAG 
CAGCTGCACA AGTACGTCCA GGAGGTCAAG AAGGCCGAGG ATTTGCTCAT CCAGAAGGAA 
AAGGAGCGCG ATGATATGCT GGATCAGTAC CACTGTCTAA CCCAGGGCCA AGCTACACTG 
GAGGGTAATA ACCAAAGTCT GGAGTGCGAG GCGGTTGAGT TCAGGCGACA GATCTGTGAG 
CTGGAGTGCG AGGTGCACAG CCTGAAGGAA CAACTGCAGC TTCGCCAGTG CGCACTTCAT 
GATATGGAGG TGCAGCTGAC CGCCGCTCGG GCTTCAGTGC GTTGTTTGGA AAGGGAGCTG 
GAGAATGCCC GAGATGACAT CCGTGTCCAG AAGGTGGATC TTGAGGCGCG CAAGGAGCTG 
TGTGATAAGT TGGATGTGGA AAGAAGCAAA CTCAATGCGG AGCTGAATGA TGTGAATGAA 
ATTCGCAAAA AGGTGAGCTT CAGCTCCGGT CCAGATCACT TTCCGTATCT GTATCCTAGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep135 ( NM_001202179.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189108.1 Drosophila melanogaster centrosomal protein 135kDa, transcript variant B (Cep135), mRNA.

Price & Availability↑

CloneID	OFa18523
Clone ID Related Accession (Same CDS sequence)	NM_001202179.2
Accession Version	NM_001202179.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	centrosomal protein 135kDa, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001202179.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATATCA ACGATGGTGA CTTTAAAGAC CTCCGCTGCA AACTGGACAT CATGGGTTTC 
ACACAGACGC TGCCCGTTTT GGCCATTCCA CTGGTTCAGG CTGTTTTCGG GGACCTAATC 
AAGACCACAG AATGTTTGAG GGATACCAAG AAAAAGGTGG CCGAGCTGCT GGAGGAAAAG 
ACCTGTTGGG AGCTGGGCGT CGAACCCTAC AAGTGTGACA ACTCTCGCCT GCTGGCCGAG 
TGCAATGAAC TGCATCTCCA GTTTTTGAGG GACAGGGAGG ACTACGAGCT GCGCCTGTGT 
GAGTCCGAGC GAAAGATCCG AAATCTGGAG TTGGACAAGC AGCATTTGCA GAGTCACAAC 
GATGGACTTC AAAGGCAACT GGACGCTTTG CTTACCAAGC AAATGGTTTC ATCAGTCACG 
AACAAAAAGG CGACAGCTGG CATTGGACGT CAGACCAAAA AACCCTTTAT AACCACAGTG 
CGTTCGGGCA TTGCGATGCC AACTACACTG GGCACTAGCA GCAGTGCTCT TAAATGCACA 
AAATGCAATG CTGGGGTATT CCAGAAGACC ACCACAACGA CCAAAGACGG TGTGACAGTG 
ACGCAAACCA GTGGACAGGA GGAGTTAGAC AAGATGCAGA ATGATCTTAC GGCAGCCGGA 
GAACAACTGG AGTTCTTCAA GCGCAAAGTG GAGGCCAGAA ATCGGGAGAT TCGCCGCTTA 
AACGACATGC TAGCAGGAGG GCGTCCTTTA GCGGCTTTGG CCAAGGATTG CTGTTACAAG 
GACGTGGGCG CCCTCTCCCA GGACATCGAT CTGTTGCAAC GCGAGAAGAG CGATTTAATG 
ATGCAAGTTC GAGAATTCCA GGACAAGATG CACGATGCTA TGCAGCGGGC TCTAAGCTCC 
GAAGAGGATA AAATAAAGCT CCAAACGCAG CTGGAGGAGC TCAAGGAAGC TGCGTTGCAA 
GTGGAACAGC AGGCCAATGC GGAAATCGAT GCCAAGGAAT CGGAACTTAG GCAACTGCAG 
TTGGAGCTTA AGAAGAAGGG AAAGGATCAC CGACTGACCG GTGGCTTTGC TTCCAATCAA 
AGTGACAAGC ATAATCTTAA TGAACGCTTA AATCTTTTGA CTCGTCGTGA AGAGGAACTA 
CAGGCCACAA ATGAAAAGCA TAAGAAGAAA ATTCAGAAAA TGCAAGCAAA GATATTAGAA 
CTGCAGAAGG AGCTCAAAGA TCAGAATAAA CATTCCAACG TCACCCTGGA TGAAGAGAAA 
ATACGCCTGT CATCAGAGCG GGACTTCTTC CAGAAGGAGT ATCTGCGTCT GATGAGCAAG 
ACCGGATCCG AGTCGGAGAT CGCCTTCTTG CATGCCCAAA TTAAATCTAA GGACGAGGAG 
TTAAAGGCCC TGCGATCTGA ACTGTTTCAT GGAGGAAAGC AGCAATTTTC CCCACAAAAA 
AGTGTGCAGT ATGAGACACT GCCGCCCCCC ACAGCTTCAT CCATTACCTC CACCGTTACT 
AGCAATACGA GTGACTGCGT CCAGGCGGCC ATTGCAAGGG TGGAGCGGGA ACGGGATTGC 
GCCAGGACCG AGCTAGAAAG GGTTCGTTGC GAGCGGGATA CGCTGAGGGA GAAGCAACTG 
AGCACTGTTC AACTTCATGC CGATGAGCTA CAGGCTCTGC GCCTGCGAAA CGAAGAGCTT 
AACGATCGCC TGCGGCAAAT GGAACGCGAT AATCGGGAAC TTAACTCGGC TCGTCTGCCA 
ACTGAAACGA ATCTGGTGCT GCTTAAGGAG GACTTGTTGC AAATGAGGCA GCGTGTGGCA 
TCTATGCAAA CCGAAATTGA CCAACTAAAG ACCGAAAATG ATCAAATAAC CATGCTCAAC 
GATCAAAACG AGCGCATCAT AGCGGATTAT CAGAGTAAAC TTTTGGTGGC CGAGCGTCAA 
CGACAGTCGG CGGATGTTCG GGCCAGCACC TTGGACTCCA GTCGGGAATC CAATCGCAGC 
GAGGTTACCC AACTCCGCAT GGACTTAGGA GCTCTGCGGC AGACCTATAT ATCGTTAGAA 
CACGAGAAGG ATACGCTTCT GCATCAATTG GATACCAAAA CTGAGCGGGT ATACAAACTG 
GAGTATGAGC TAAAGGATTG CAAAGAAAAA CGGAATGCCC TAGAGCAGAA TGTGAAGGAC 
CTGGAGGATC AATTGCGAAA ACTGGCGAAC AGGAACCGGC AGCGCGACTC AGAACTGACG 
GAGACCTCGA CGGAAAGTAA AACACTGCGC CAGCAGATTG TGGCACTCAA AGCCAGTCGC 
GATGAGGCAA TAGCAGAGAA TCGCCGGCTG ATGGACAAAT TGAGCGATGC CCAAGTGGAG 
GCCAGGACGT TGCAGAAGAA GCTGACGGAT TCCGAGCTTC AGGTGGCCAA CATGAAGCAG 
CAGCTGCACA AGTACGTCCA GGAGGTCAAG AAGGCCGAGG ATTTGCTCAT CCAGAAGGTG 
TCCTCTAAAT TCAAGGAAAA GGAGCGCGAT GATATGCTGG ATCAGTACCA CTGTCTAACC 
CAGGGCCAAG CTACACTGGA GGGTAATAAC CAAAGTCTGG AGTGCGAGGC GGTTGAGTTC 
AGGCGACAGA TCTGTGAGCT GGAGTGCGAG GTGCACAGCC TGAAGGAACA ACTGCAGCTT 
CGCCAGTGCG CACTTCATGA TATGGAGGTG CAGCTGACCG CCGCTCGGGC TTCAGTGCGT 
TGTTTGGAAA GGGAGCTGGA GAATGCCCGA GATGACATCC GTGTCCAGAA GGTGGATCTT 
GAGGCGCGCA AGGAGCTGTG TGATAAGTTG GATGTGGAAA GAAGCAAACT CAATGCGGAG 
CTGAATGATG TGAATGAAAT TCGCAAAAAG CTTGAAAAAC AATGCGAAAA ACTGCGCGAT 
GAGCTTCAGC AGAGCTTGGC CATTAATCAG GTCACCAACG AAACCACTGA TCTCATGCTC 
GGGCGCTTGC ACAATGATCA GCAGCATCAG GAGGACGATG ATATTAGGTC CAAACATGAA 
ATGGACCGAT TGCAACGTCA GCTCCAGCAA ACATTGGATC AGTTGCAGGA GGAGCGTGTC 
CGCTGCAGGC ACCACGAGGA GTTGGCTGAA AAGTGGGAGC AACAGGTACG CGATCTGCGT 
CGAAATCTCG CCGATGATCG CTACAATCAG GCTAGGACAC GTGAGGTTAG CCCCCGGGTG 
CCATCGAAGA CTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189108.1
CDS	125..3322
Translation	MNINDGDFKDLRCKLDIMGFTQTLPVLAIPLVQAVFGDLIKTTECLRDTKKKVAELLEEKTCWELGVEPYKCDNSRLLAECNELHLQFLRDREDYELRLCESERKIRNLELDKQHLQSHNDGLQRQLDALLTKQMVSSVTNKKATAGIGRQTKKPFITTVRSGIAMPTTLGTSSSALKCTKCNAGVFQKTTTTTKDGVTVTQTSGQEELDKMQNDLTAAGEQLEFFKRKVEARNREIRRLNDMLAGGRPLAALAKDCCYKDVGALSQDIDLLQREKSDLMMQVREFQDKMHDAMQRALSSEEDKIKLQTQLEELKEAALQVEQQANAEIDAKESELRQLQLELKKKGKDHRLTGGFASNQSDKHNLNERLNLLTRREEELQATNEKHKKKIQKMQAKILELQKELKDQNKHSNVTLDEEKIRLSSERDFFQKEYLRLMSKTGSESEIAFLHAQIKSKDEELKALRSELFHGGKQQFSPQKSVQYETLPPPTASSITSTVTSNTSDCVQAAIARVERERDCARTELERVRCERDTLREKQLSTVQLHADELQALRLRNEELNDRLRQMERDNRELNSARLPTETNLVLLKEDLLQMRQRVASMQTEIDQLKTENDQITMLNDQNERIIADYQSKLLVAERQRQSADVRASTLDSSRESNRSEVTQLRMDLGALRQTYISLEHEKDTLLHQLDTKTERVYKLEYELKDCKEKRNALEQNVKDLEDQLRKLANRNRQRDSELTETSTESKTLRQQIVALKASRDEAIAENRRLMDKLSDAQVEARTLQKKLTDSELQVANMKQQLHKYVQEVKKAEDLLIQKVSSKFKEKERDDMLDQYHCLTQGQATLEGNNQSLECEAVEFRRQICELECEVHSLKEQLQLRQCALHDMEVQLTAARASVRCLERELENARDDIRVQKVDLEARKELCDKLDVERSKLNAELNDVNEIRKKLEKQCEKLRDELQQSLAINQVTNETTDLMLGRLHNDQQHQEDDDIRSKHEMDRLQRQLQQTLDQLQEERVRCRHHEELAEKWEQQVRDLRRNLADDRYNQARTREVSPRVPSKTL

Target ORF information:

RefSeq Version	NM_001202179.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster centrosomal protein 135kDa, transcript variant B (Cep135), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202179.2

ORF Insert Sequence:

ATGAATATCA ACGATGGTGA CTTTAAAGAC CTCCGCTGCA AACTGGACAT CATGGGTTTC 
ACACAGACGC TGCCCGTTTT GGCCATTCCA CTGGTTCAGG CTGTTTTCGG GGACCTAATC 
AAGACCACAG AATGTTTGAG GGATACCAAG AAAAAGGTGG CCGAGCTGCT GGAGGAAAAG 
ACCTGTTGGG AGCTGGGCGT CGAACCCTAC AAGTGTGACA ACTCTCGCCT GCTGGCCGAG 
TGCAATGAAC TGCATCTCCA GTTTTTGAGG GACAGGGAGG ACTACGAGCT GCGCCTGTGT 
GAGTCCGAGC GAAAGATCCG AAATCTGGAG TTGGACAAGC AGCATTTGCA GAGTCACAAC 
GATGGACTTC AAAGGCAACT GGACGCTTTG CTTACCAAGC AAATGGTTTC ATCAGTCACG 
AACAAAAAGG CGACAGCTGG CATTGGACGT CAGACCAAAA AACCCTTTAT AACCACAGTG 
CGTTCGGGCA TTGCGATGCC AACTACACTG GGCACTAGCA GCAGTGCTCT TAAATGCACA 
AAATGCAATG CTGGGGTATT CCAGAAGACC ACCACAACGA CCAAAGACGG TGTGACAGTG 
ACGCAAACCA GTGGACAGGA GGAGTTAGAC AAGATGCAGA ATGATCTTAC GGCAGCCGGA 
GAACAACTGG AGTTCTTCAA GCGCAAAGTG GAGGCCAGAA ATCGGGAGAT TCGCCGCTTA 
AACGACATGC TAGCAGGAGG GCGTCCTTTA GCGGCTTTGG CCAAGGATTG CTGTTACAAG 
GACGTGGGCG CCCTCTCCCA GGACATCGAT CTGTTGCAAC GCGAGAAGAG CGATTTAATG 
ATGCAAGTTC GAGAATTCCA GGACAAGATG CACGATGCTA TGCAGCGGGC TCTAAGCTCC 
GAAGAGGATA AAATAAAGCT CCAAACGCAG CTGGAGGAGC TCAAGGAAGC TGCGTTGCAA 
GTGGAACAGC AGGCCAATGC GGAAATCGAT GCCAAGGAAT CGGAACTTAG GCAACTGCAG 
TTGGAGCTTA AGAAGAAGGG AAAGGATCAC CGACTGACCG GTGGCTTTGC TTCCAATCAA 
AGTGACAAGC ATAATCTTAA TGAACGCTTA AATCTTTTGA CTCGTCGTGA AGAGGAACTA 
CAGGCCACAA ATGAAAAGCA TAAGAAGAAA ATTCAGAAAA TGCAAGCAAA GATATTAGAA 
CTGCAGAAGG AGCTCAAAGA TCAGAATAAA CATTCCAACG TCACCCTGGA TGAAGAGAAA 
ATACGCCTGT CATCAGAGCG GGACTTCTTC CAGAAGGAGT ATCTGCGTCT GATGAGCAAG 
ACCGGATCCG AGTCGGAGAT CGCCTTCTTG CATGCCCAAA TTAAATCTAA GGACGAGGAG 
TTAAAGGCCC TGCGATCTGA ACTGTTTCAT GGAGGAAAGC AGCAATTTTC CCCACAAAAA 
AGTGTGCAGT ATGAGACACT GCCGCCCCCC ACAGCTTCAT CCATTACCTC CACCGTTACT 
AGCAATACGA GTGACTGCGT CCAGGCGGCC ATTGCAAGGG TGGAGCGGGA ACGGGATTGC 
GCCAGGACCG AGCTAGAAAG GGTTCGTTGC GAGCGGGATA CGCTGAGGGA GAAGCAACTG 
AGCACTGTTC AACTTCATGC CGATGAGCTA CAGGCTCTGC GCCTGCGAAA CGAAGAGCTT 
AACGATCGCC TGCGGCAAAT GGAACGCGAT AATCGGGAAC TTAACTCGGC TCGTCTGCCA 
ACTGAAACGA ATCTGGTGCT GCTTAAGGAG GACTTGTTGC AAATGAGGCA GCGTGTGGCA 
TCTATGCAAA CCGAAATTGA CCAACTAAAG ACCGAAAATG ATCAAATAAC CATGCTCAAC 
GATCAAAACG AGCGCATCAT AGCGGATTAT CAGAGTAAAC TTTTGGTGGC CGAGCGTCAA 
CGACAGTCGG CGGATGTTCG GGCCAGCACC TTGGACTCCA GTCGGGAATC CAATCGCAGC 
GAGGTTACCC AACTCCGCAT GGACTTAGGA GCTCTGCGGC AGACCTATAT ATCGTTAGAA 
CACGAGAAGG ATACGCTTCT GCATCAATTG GATACCAAAA CTGAGCGGGT ATACAAACTG 
GAGTATGAGC TAAAGGATTG CAAAGAAAAA CGGAATGCCC TAGAGCAGAA TGTGAAGGAC 
CTGGAGGATC AATTGCGAAA ACTGGCGAAC AGGAACCGGC AGCGCGACTC AGAACTGACG 
GAGACCTCGA CGGAAAGTAA AACACTGCGC CAGCAGATTG TGGCACTCAA AGCCAGTCGC 
GATGAGGCAA TAGCAGAGAA TCGCCGGCTG ATGGACAAAT TGAGCGATGC CCAAGTGGAG 
GCCAGGACGT TGCAGAAGAA GCTGACGGAT TCCGAGCTTC AGGTGGCCAA CATGAAGCAG 
CAGCTGCACA AGTACGTCCA GGAGGTCAAG AAGGCCGAGG ATTTGCTCAT CCAGAAGGTG 
TCCTCTAAAT TCAAGGAAAA GGAGCGCGAT GATATGCTGG ATCAGTACCA CTGTCTAACC 
CAGGGCCAAG CTACACTGGA GGGTAATAAC CAAAGTCTGG AGTGCGAGGC GGTTGAGTTC 
AGGCGACAGA TCTGTGAGCT GGAGTGCGAG GTGCACAGCC TGAAGGAACA ACTGCAGCTT 
CGCCAGTGCG CACTTCATGA TATGGAGGTG CAGCTGACCG CCGCTCGGGC TTCAGTGCGT 
TGTTTGGAAA GGGAGCTGGA GAATGCCCGA GATGACATCC GTGTCCAGAA GGTGGATCTT 
GAGGCGCGCA AGGAGCTGTG TGATAAGTTG GATGTGGAAA GAAGCAAACT CAATGCGGAG 
CTGAATGATG TGAATGAAAT TCGCAAAAAG CTTGAAAAAC AATGCGAAAA ACTGCGCGAT 
GAGCTTCAGC AGAGCTTGGC CATTAATCAG GTCACCAACG AAACCACTGA TCTCATGCTC 
GGGCGCTTGC ACAATGATCA GCAGCATCAG GAGGACGATG ATATTAGGTC CAAACATGAA 
ATGGACCGAT TGCAACGTCA GCTCCAGCAA ACATTGGATC AGTTGCAGGA GGAGCGTGTC 
CGCTGCAGGC ACCACGAGGA GTTGGCTGAA AAGTGGGAGC AACAGGTACG CGATCTGCGT 
CGAAATCTCG CCGATGATCG CTACAATCAG GCTAGGACAC GTGAGGTTAG CCCCCGGGTG 
CCATCGAAGA CTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cep135 ( NM_140492.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648749.3 Drosophila melanogaster centrosomal protein 135kDa, transcript variant A (Cep135), mRNA.

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CloneID	OFa20234
Clone ID Related Accession (Same CDS sequence)	NM_140492.4
Accession Version	NM_140492.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	centrosomal protein 135kDa, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140492.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATATCA ACGATGGTGA CTTTAAAGAC CTCCGCTGCA AACTGGACAT CATGGGTTTC 
ACACAGACGC TGCCCGTTTT GGCCATTCCA CTGGTTCAGG CTGTTTTCGG GGACCTAATC 
AAGACCACAG AATGTTTGAG GGATACCAAG AAAAAGGTGG CCGAGCTGCT GGAGGAAAAG 
ACCTGTTGGG AGCTGGGCGT CGAACCCTAC AAGTGTGACA ACTCTCGCCT GCTGGCCGAG 
TGCAATGAAC TGCATCTCCA GTTTTTGAGG GACAGGGAGG ACTACGAGCT GCGCCTGTGT 
GAGTCCGAGC GAAAGATCCG AAATCTGGAG TTGGACAAGC AGCATTTGCA GAGTCACAAC 
GATGGACTTC AAAGGCAACT GGACGCTTTG CTTACCAAGC AAATGGTTTC ATCAGTCACG 
AACAAAAAGG CGACAGCTGG CATTGGACGT CAGACCAAAA AACCCTTTAT AACCACAGTG 
CGTTCGGGCA TTGCGATGCC AACTACACTG GGCACTAGCA GCAGTGCTCT TAAATGCACA 
AAATGCAATG CTGGGGTATT CCAGAAGACC ACCACAACGA CCAAAGACGG TGTGACAGTG 
ACGCAAACCA GTGGACAGGA GGAGTTAGAC AAGATGCAGA ATGATCTTAC GGCAGCCGGA 
GAACAACTGG AGTTCTTCAA GCGCAAAGTG GAGGCCAGAA ATCGGGAGAT TCGCCGCTTA 
AACGACATGC TAGCAGGAGG GCGTCCTTTA GCGGCTTTGG CCAAGGATTG CTGTTACAAG 
GACGTGGGCG CCCTCTCCCA GGACATCGAT CTGTTGCAAC GCGAGAAGAG CGATTTAATG 
ATGCAAGTTC GAGAATTCCA GGACAAGATG CACGATGCTA TGCAGCGGGC TCTAAGCTCC 
GAAGAGGATA AAATAAAGCT CCAAACGCAG CTGGAGGAGC TCAAGGAAGC TGCGTTGCAA 
GTGGAACAGC AGGCCAATGC GGAAATCGAT GCCAAGGAAT CGGAACTTAG GCAACTGCAG 
TTGGAGCTTA AGAAGAAGGG AAAGGATCAC CGACTGACCG GTGGCTTTGC TTCCAATCAA 
AGTGACAAGC ATAATCTTAA TGAACGCTTA AATCTTTTGA CTCGTCGTGA AGAGGAACTA 
CAGGCCACAA ATGAAAAGCA TAAGAAGAAA ATTCAGAAAA TGCAAGCAAA GATATTAGAA 
CTGCAGAAGG AGCTCAAAGA TCAGAATAAA CATTCCAACG TCACCCTGGA TGAAGAGAAA 
ATACGCCTGT CATCAGAGCG GGACTTCTTC CAGAAGGAGT ATCTGCGTCT GATGAGCAAG 
ACCGGATCCG AGTCGGAGAT CGCCTTCTTG CATGCCCAAA TTAAATCTAA GGACGAGGAG 
TTAAAGGCCC TGCGATCTGA ACTGTTTCAT GGAGGAAAGC AGCAATTTTC CCCACAAAAA 
AGTGTGCAGT ATGAGACACT GCCGCCCCCC ACAGCTTCAT CCATTACCTC CACCGTTACT 
AGCAATACGA GTGACTGCGT CCAGGCGGCC ATTGCAAGGG TGGAGCGGGA ACGGGATTGC 
GCCAGGACCG AGCTAGAAAG GGTTCGTTGC GAGCGGGATA CGCTGAGGGA GAAGCAACTG 
AGCACTGTTC AACTTCATGC CGATGAGCTA CAGGCTCTGC GCCTGCGAAA CGAAGAGCTT 
AACGATCGCC TGCGGCAAAT GGAACGCGAT AATCGGGAAC TTAACTCGGC TCGTCTGCCA 
ACTGAAACGA ATCTGGTGCT GCTTAAGGAG GACTTGTTGC AAATGAGGCA GCGTGTGGCA 
TCTATGCAAA CCGAAATTGA CCAACTAAAG ACCGAAAATG ATCAAATAAC CATGCTCAAC 
GATCAAAACG AGCGCATCAT AGCGGATTAT CAGAGTAAAC TTTTGGTGGC CGAGCGTCAA 
CGACAGTCGG CGGATGTTCG GGCCAGCACC TTGGACTCCA GTCGGGAATC CAATCGCAGC 
GAGGTTACCC AACTCCGCAT GGACTTAGGA GCTCTGCGGC AGACCTATAT ATCGTTAGAA 
CACGAGAAGG ATACGCTTCT GCATCAATTG GATACCAAAA CTGAGCGGGT ATACAAACTG 
GAGTATGAGC TAAAGGATTG CAAAGAAAAA CGGAATGCCC TAGAGCAGAA TGTGAAGGAC 
CTGGAGGATC AATTGCGAAA ACTGGCGAAC AGGAACCGGC AGCGCGACTC AGAACTGACG 
GAGACCTCGA CGGAAAGTAA AACACTGCGC CAGCAGATTG TGGCACTCAA AGCCAGTCGC 
GATGAGGCAA TAGCAGAGAA TCGCCGGCTG ATGGACAAAT TGAGCGATGC CCAAGTGGAG 
GCCAGGACGT TGCAGAAGAA GCTGACGGAT TCCGAGCTTC AGGTGGCCAA CATGAAGCAG 
CAGCTGCACA AGTACGTCCA GGAGGTCAAG AAGGCCGAGG ATTTGCTCAT CCAGAAGGAA 
AAGGAGCGCG ATGATATGCT GGATCAGTAC CACTGTCTAA CCCAGGGCCA AGCTACACTG 
GAGGGTAATA ACCAAAGTCT GGAGTGCGAG GCGGTTGAGT TCAGGCGACA GATCTGTGAG 
CTGGAGTGCG AGGTGCACAG CCTGAAGGAA CAACTGCAGC TTCGCCAGTG CGCACTTCAT 
GATATGGAGG TGCAGCTGAC CGCCGCTCGG GCTTCAGTGC GTTGTTTGGA AAGGGAGCTG 
GAGAATGCCC GAGATGACAT CCGTGTCCAG AAGGTGGATC TTGAGGCGCG CAAGGAGCTG 
TGTGATAAGT TGGATGTGGA AAGAAGCAAA CTCAATGCGG AGCTGAATGA TGTGAATGAA 
ATTCGCAAAA AGCTTGAAAA ACAATGCGAA AAACTGCGCG ATGAGCTTCA GCAGAGCTTG 
GCCATTAATC AGGTCACCAA CGAAACCACT GATCTCATGC TCGGGCGCTT GCACAATGAT 
CAGCAGCATC AGGAGGACGA TGATATTAGG TCCAAACATG AAATGGACCG ATTGCAACGT 
CAGCTCCAGC AAACATTGGA TCAGTTGCAG GAGGAGCGTG TCCGCTGCAG GCACCACGAG 
GAGTTGGCTG AAAAGTGGGA GCAACAGGTA CGCGATCTGC GTCGAAATCT CGCCGATGAT 
CGCTACAATC AGGCTAGGAC ACGTGAGGTT AGCCCCCGGG TGCCATCGAA GACTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648749.3
CDS	125..3304
Translation	MNINDGDFKDLRCKLDIMGFTQTLPVLAIPLVQAVFGDLIKTTECLRDTKKKVAELLEEKTCWELGVEPYKCDNSRLLAECNELHLQFLRDREDYELRLCESERKIRNLELDKQHLQSHNDGLQRQLDALLTKQMVSSVTNKKATAGIGRQTKKPFITTVRSGIAMPTTLGTSSSALKCTKCNAGVFQKTTTTTKDGVTVTQTSGQEELDKMQNDLTAAGEQLEFFKRKVEARNREIRRLNDMLAGGRPLAALAKDCCYKDVGALSQDIDLLQREKSDLMMQVREFQDKMHDAMQRALSSEEDKIKLQTQLEELKEAALQVEQQANAEIDAKESELRQLQLELKKKGKDHRLTGGFASNQSDKHNLNERLNLLTRREEELQATNEKHKKKIQKMQAKILELQKELKDQNKHSNVTLDEEKIRLSSERDFFQKEYLRLMSKTGSESEIAFLHAQIKSKDEELKALRSELFHGGKQQFSPQKSVQYETLPPPTASSITSTVTSNTSDCVQAAIARVERERDCARTELERVRCERDTLREKQLSTVQLHADELQALRLRNEELNDRLRQMERDNRELNSARLPTETNLVLLKEDLLQMRQRVASMQTEIDQLKTENDQITMLNDQNERIIADYQSKLLVAERQRQSADVRASTLDSSRESNRSEVTQLRMDLGALRQTYISLEHEKDTLLHQLDTKTERVYKLEYELKDCKEKRNALEQNVKDLEDQLRKLANRNRQRDSELTETSTESKTLRQQIVALKASRDEAIAENRRLMDKLSDAQVEARTLQKKLTDSELQVANMKQQLHKYVQEVKKAEDLLIQKEKERDDMLDQYHCLTQGQATLEGNNQSLECEAVEFRRQICELECEVHSLKEQLQLRQCALHDMEVQLTAARASVRCLERELENARDDIRVQKVDLEARKELCDKLDVERSKLNAELNDVNEIRKKLEKQCEKLRDELQQSLAINQVTNETTDLMLGRLHNDQQHQEDDDIRSKHEMDRLQRQLQQTLDQLQEERVRCRHHEELAEKWEQQVRDLRRNLADDRYNQARTREVSPRVPSKTL

Target ORF information:

RefSeq Version	NM_140492.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster centrosomal protein 135kDa, transcript variant A (Cep135), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140492.4

ORF Insert Sequence:

ATGAATATCA ACGATGGTGA CTTTAAAGAC CTCCGCTGCA AACTGGACAT CATGGGTTTC 
ACACAGACGC TGCCCGTTTT GGCCATTCCA CTGGTTCAGG CTGTTTTCGG GGACCTAATC 
AAGACCACAG AATGTTTGAG GGATACCAAG AAAAAGGTGG CCGAGCTGCT GGAGGAAAAG 
ACCTGTTGGG AGCTGGGCGT CGAACCCTAC AAGTGTGACA ACTCTCGCCT GCTGGCCGAG 
TGCAATGAAC TGCATCTCCA GTTTTTGAGG GACAGGGAGG ACTACGAGCT GCGCCTGTGT 
GAGTCCGAGC GAAAGATCCG AAATCTGGAG TTGGACAAGC AGCATTTGCA GAGTCACAAC 
GATGGACTTC AAAGGCAACT GGACGCTTTG CTTACCAAGC AAATGGTTTC ATCAGTCACG 
AACAAAAAGG CGACAGCTGG CATTGGACGT CAGACCAAAA AACCCTTTAT AACCACAGTG 
CGTTCGGGCA TTGCGATGCC AACTACACTG GGCACTAGCA GCAGTGCTCT TAAATGCACA 
AAATGCAATG CTGGGGTATT CCAGAAGACC ACCACAACGA CCAAAGACGG TGTGACAGTG 
ACGCAAACCA GTGGACAGGA GGAGTTAGAC AAGATGCAGA ATGATCTTAC GGCAGCCGGA 
GAACAACTGG AGTTCTTCAA GCGCAAAGTG GAGGCCAGAA ATCGGGAGAT TCGCCGCTTA 
AACGACATGC TAGCAGGAGG GCGTCCTTTA GCGGCTTTGG CCAAGGATTG CTGTTACAAG 
GACGTGGGCG CCCTCTCCCA GGACATCGAT CTGTTGCAAC GCGAGAAGAG CGATTTAATG 
ATGCAAGTTC GAGAATTCCA GGACAAGATG CACGATGCTA TGCAGCGGGC TCTAAGCTCC 
GAAGAGGATA AAATAAAGCT CCAAACGCAG CTGGAGGAGC TCAAGGAAGC TGCGTTGCAA 
GTGGAACAGC AGGCCAATGC GGAAATCGAT GCCAAGGAAT CGGAACTTAG GCAACTGCAG 
TTGGAGCTTA AGAAGAAGGG AAAGGATCAC CGACTGACCG GTGGCTTTGC TTCCAATCAA 
AGTGACAAGC ATAATCTTAA TGAACGCTTA AATCTTTTGA CTCGTCGTGA AGAGGAACTA 
CAGGCCACAA ATGAAAAGCA TAAGAAGAAA ATTCAGAAAA TGCAAGCAAA GATATTAGAA 
CTGCAGAAGG AGCTCAAAGA TCAGAATAAA CATTCCAACG TCACCCTGGA TGAAGAGAAA 
ATACGCCTGT CATCAGAGCG GGACTTCTTC CAGAAGGAGT ATCTGCGTCT GATGAGCAAG 
ACCGGATCCG AGTCGGAGAT CGCCTTCTTG CATGCCCAAA TTAAATCTAA GGACGAGGAG 
TTAAAGGCCC TGCGATCTGA ACTGTTTCAT GGAGGAAAGC AGCAATTTTC CCCACAAAAA 
AGTGTGCAGT ATGAGACACT GCCGCCCCCC ACAGCTTCAT CCATTACCTC CACCGTTACT 
AGCAATACGA GTGACTGCGT CCAGGCGGCC ATTGCAAGGG TGGAGCGGGA ACGGGATTGC 
GCCAGGACCG AGCTAGAAAG GGTTCGTTGC GAGCGGGATA CGCTGAGGGA GAAGCAACTG 
AGCACTGTTC AACTTCATGC CGATGAGCTA CAGGCTCTGC GCCTGCGAAA CGAAGAGCTT 
AACGATCGCC TGCGGCAAAT GGAACGCGAT AATCGGGAAC TTAACTCGGC TCGTCTGCCA 
ACTGAAACGA ATCTGGTGCT GCTTAAGGAG GACTTGTTGC AAATGAGGCA GCGTGTGGCA 
TCTATGCAAA CCGAAATTGA CCAACTAAAG ACCGAAAATG ATCAAATAAC CATGCTCAAC 
GATCAAAACG AGCGCATCAT AGCGGATTAT CAGAGTAAAC TTTTGGTGGC CGAGCGTCAA 
CGACAGTCGG CGGATGTTCG GGCCAGCACC TTGGACTCCA GTCGGGAATC CAATCGCAGC 
GAGGTTACCC AACTCCGCAT GGACTTAGGA GCTCTGCGGC AGACCTATAT ATCGTTAGAA 
CACGAGAAGG ATACGCTTCT GCATCAATTG GATACCAAAA CTGAGCGGGT ATACAAACTG 
GAGTATGAGC TAAAGGATTG CAAAGAAAAA CGGAATGCCC TAGAGCAGAA TGTGAAGGAC 
CTGGAGGATC AATTGCGAAA ACTGGCGAAC AGGAACCGGC AGCGCGACTC AGAACTGACG 
GAGACCTCGA CGGAAAGTAA AACACTGCGC CAGCAGATTG TGGCACTCAA AGCCAGTCGC 
GATGAGGCAA TAGCAGAGAA TCGCCGGCTG ATGGACAAAT TGAGCGATGC CCAAGTGGAG 
GCCAGGACGT TGCAGAAGAA GCTGACGGAT TCCGAGCTTC AGGTGGCCAA CATGAAGCAG 
CAGCTGCACA AGTACGTCCA GGAGGTCAAG AAGGCCGAGG ATTTGCTCAT CCAGAAGGAA 
AAGGAGCGCG ATGATATGCT GGATCAGTAC CACTGTCTAA CCCAGGGCCA AGCTACACTG 
GAGGGTAATA ACCAAAGTCT GGAGTGCGAG GCGGTTGAGT TCAGGCGACA GATCTGTGAG 
CTGGAGTGCG AGGTGCACAG CCTGAAGGAA CAACTGCAGC TTCGCCAGTG CGCACTTCAT 
GATATGGAGG TGCAGCTGAC CGCCGCTCGG GCTTCAGTGC GTTGTTTGGA AAGGGAGCTG 
GAGAATGCCC GAGATGACAT CCGTGTCCAG AAGGTGGATC TTGAGGCGCG CAAGGAGCTG 
TGTGATAAGT TGGATGTGGA AAGAAGCAAA CTCAATGCGG AGCTGAATGA TGTGAATGAA 
ATTCGCAAAA AGCTTGAAAA ACAATGCGAA AAACTGCGCG ATGAGCTTCA GCAGAGCTTG 
GCCATTAATC AGGTCACCAA CGAAACCACT GATCTCATGC TCGGGCGCTT GCACAATGAT 
CAGCAGCATC AGGAGGACGA TGATATTAGG TCCAAACATG AAATGGACCG ATTGCAACGT 
CAGCTCCAGC AAACATTGGA TCAGTTGCAG GAGGAGCGTG TCCGCTGCAG GCACCACGAG 
GAGTTGGCTG AAAAGTGGGA GCAACAGGTA CGCGATCTGC GTCGAAATCT CGCCGATGAT 
CGCTACAATC AGGCTAGGAC ACGTGAGGTT AGCCCCCGGG TGCCATCGAA GACTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cep135 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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