dlp cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dlp
Entrez Gene ID	39596
Full Name	dally-like
Synonyms	CG17703,CG32146,CG5031,CT16138,D-gpcB,DLP,Dally-like,Dlp,Dly,Dmel\CG32146,dly
General protein information	Preferred Names dally-like Names CG32146 gene product from transcript CG32146-RA CG32146-PA CG32146-PC D-glypican B Dally-like Dally-like protein dallylike dlp-PA dlp-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 70E5-70E7

mRNA and Protein(s)

mRNA	Protein	Name
NM_079347.3	NP_524071.2	dally-like, isoform A
NM_206353.2	NP_996075.2	dally-like, isoform C

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gpc4	NP_001014130.1
Bos taurus (cattle)	GPC4	NP_001192713.1
Mus musculus (house mouse)	Gpc4	NP_032176.2
Gallus gallus (chicken)	GPC4	XP_420222.3
Danio rerio (zebrafish)	gpc4	NP_571935.2
Xenopus tropicalis (tropical clawed frog)	gpc4	XP_002932634.1
Canis lupus familiaris (dog)	GPC4	XP_549265.2
	GPC4	XP_001097252.1
Drosophila melanogaster (fruit fly)	dlp	NP_996075.2
Caenorhabditis elegans (roundworm)	gpn-1	NP_510582.1
Homo sapiens (human)	GPC4	NP_001439.2
Pan troglodytes (chimpanzee)	GPC4	XP_529161.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003559	XP_313306.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DLP and ASF1 are part of a predeposition complex, which is recruited by XNP and is necessary to prevent DNA exposure in the nucleus.
Title: The Drosophila DAXX-Like Protein (DLP) Cooperates with ASF1 for H3.3 Deposition and Heterochromatin Formation.

ECM over planar cell polarity mutant cells had reduced levels of laminin, Dally and Dlp, and whereas Dpp-receiving ASP cytonemes navigated in the Dally layer and required Dally (but not Dlp), FGF-receiving ASP cytonemes navigated in the Dlp layer, requiring Dlp (but not Dally).
Title: Cells must express components of the planar cell polarity system and extracellular matrix to support cytonemes.

The authors show that Pent internalises the Dpp co-receptors, the glypicans Dally and Dally-like protein (Dlp), and propose that this internalisation is important in the establishment of a long range Dpp gradient.
Title: Pentagone internalises glypicans to fine-tune multiple signalling pathways.

data suggest that Dally and Dlp are essential regulators for Upd distribution and JAK/STAT signaling activity in eye development
Title: Drosophila glypicans Dally and Dally-like are essential regulators for JAK/STAT signaling and Unpaired distribution in eye development.

findings demonstrate that the glypican dlp regulates formation of the follicular gradient of the Upd morphogen, Upd
Title: Glypicans regulate JAK/STAT signaling and distribution of the Unpaired morphogen.

The following dlp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dlp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa20192	NM_079347.3 Latest version!	Drosophila melanogaster dally-like (dlp), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa20193	NM_206353.2 Latest version!	Drosophila melanogaster dally-like (dlp), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dlp ( NM_079347.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524071.2 Drosophila melanogaster dally-like (dlp), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa20192
Clone ID Related Accession (Same CDS sequence)	NM_079347.3
Accession Version	NM_079347.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dally-like, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079347.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTACATC AGCAGCAACA ACAACAACAT CTGCATTGCA GAAGAAAAGC TACAGCAACA 
ACAACTGCCC GCCTTGTGAT ATTCAGCAGT CCTCTTCTAC TATTACTACT CACCACCCAC 
TTGCCGCCCA CCTTACAAGC GGACAATGGC CCCGCCCCCC AAGTGGCCGC CCTTGCCGCC 
CCCAATCCGG CGGGTGGCGT AGCAGGGTCT TCGATCATCG ATCAATTCAG TCCGAATTGC 
AGTGCTGTGA CACACATTTT CCAAGCGAGA GGAATCGATG CCATCGAAAT TCCCCAAAAA 
CCCAGTAACG AACGAGTCCT ACGATATTGC GAGTCCCCTT CGGTGGGCAC TTGTTGCACC 
TACAACATGG AGACCCGCAT GGCGATGCAA TCCCGCCAGC AGCTCGAAGG GCACACCAAG 
GACCAGATCT CTCGTATGTC TGGAATCCTG GGCAGCAAGG CCACAAAATT CAAAGATATT 
TTCACGGCCC TGCTCAAAGA GTCGCGAACG CAGTTCAATA GTATGTTCAT CCGAACATAC 
GGCGTCATTT ACGAGAGAAA TTCGTACGTC TTCTCGGATC TGTTCAAGGA GTTGGAAACC 
TACTTCGCCA ACGGTCGCGT GGACTTGCTG GAGGTCATGG ACAAGTTCTT CAATACGCTA 
TACCAGAAGA TGTTCACCGT GCTGAACACC CAGTACACAT TTGATGAAAA CTACATGCGA 
TGCGTCAGCG AGCACATGAA GGAGCTGAAG CCTTTCGGAG ATGTTCCGGA CAAGCTGTCC 
GTGCAAATCA AGCGATCCTT TGTGGCCACG CGCACCTATG GCCAGGCTCT GACCACCGCT 
TCGGAGGTGG CGAAGAAGGT CCTCAATGTG CGACTGAATG CCGATTGTAC TGGGGCTCTT 
ACCAAGATGC AGCACTGTGG TGCTTGCAAG GGATATACGG AAAAGCCCTG TACCAACTAC 
TGTGTGAACG TCATCAAGGG ATGCCTGCAC TATCAGCACG AGTTCGACAG CGAATGGGAG 
AACTTTGCGA TGGCCATGGA CAAGGTGGCG GAACGACTGC TGGGATCCTT CAACATTGTG 
ATGGTGGTGG AGCCACTCAA CATCAAGATC TCCGAGGCCA TCATGAACTT CCAGGATTCT 
GGCCAGGACA TCACCAACCG CGTATTCCAA GGATGCGGTA GGCCAAAGTT GAAGAAAATG 
AAACGGTCCA TCAGCCCCAA ACTGCAAGGA GTTCAAATCC TCAACGCAAG GAGTCCTGTG 
GAGGCCGATA CTCTGGACAT TGATGAAACG TTGGACGAGG CGATTGTGCT GAGAGAACGC 
AGAGCTGCTG AGCCCGGCTC ACAGGAGACA TCGGCGCAAC AGTCTCAAGA GCAAGGAGTT 
GGAAAGAGTG GAAATGGAGG AGGAGGCGGT GGCGGTAACA ACAGGCGGCA ACAGCAACGT 
CGAAAGCAGC AGCAACAGCG ACGCAAACAG CAAAACAATC GCGACGACAA CGATGACGAT 
GACAATGAGA GCGGAGGTGG ACGTGAACCG ATTCTGGACA GGATCGTGAG GGACATTCGC 
CAGCGAGTGA AGGACTACAA AAAGTTCTGG TCCAATCTGC CGCACTCGGT TTGTAGCAAC 
GAGGATATAG CCTCCAGTTC CGATGTGGAC GGCATGTGCT GGAATGGTCA CACAATTGAT 
AGATACATGC ACTCAATCAC CACAGAACAC GGCTCGAATC CGGAGTTTAC AGGCAATCCT 
GCCAGTACTA AGCAAACAGC TCAAATGGCC TCTCAACTAA GTCATCTGAA GAATGCCATT 
GTCCACCTTC GAAATGCCTA CAACGGCCAG GATGTTGAGT GGAGCGAACA AGAAGAACTG 
CCATATGCGG GTAGTGGAGC TGGTTCTGGA TCCGGGTCCG AAGACGACGA GGACGATGAC 
GAGGGCTCTG GCCTGGGACC CTTCGAACCC AGCCATAAGC CGGACGTGGA GCGTCCTTCG 
GTGGATGCAG ACAACGACGA TGACGAGGAC GCCGGTGGAC GGGGTCACAT GCCCACGCAC 
ACATCTCGAC CGACGAGCGG AGTGGACGAC AAGAATCCGC TCATCCACAC CACTCACTTC 
GACCAAGATC ACAACGATCT GGATGAGGAT CATAGGCAGC TGGATGAGGA TGAGGACACG 
GATGCAGGCC ATGACGGGGC CAATGACAAT CGATCATCCG ATGCCCCAGA AAAGATGACG 
CTGCGTCGCG CTCTGGTTGT TTATCTGCTG CCCCTTTATA TGGCGTGGTT CGGTGGCGTC 
TGCGCCGATT TGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524071.2
CDS	473..2770
Translation	MLHQQQQQQHLHCRRKATATTTARLVIFSSPLLLLLLTTHLPPTLQADNGPAPQVAALAAPNPAGGVAGSSIIDQFSPNCSAVTHIFQARGIDAIEIPQKPSNERVLRYCESPSVGTCCTYNMETRMAMQSRQQLEGHTKDQISRMSGILGSKATKFKDIFTALLKESRTQFNSMFIRTYGVIYERNSYVFSDLFKELETYFANGRVDLLEVMDKFFNTLYQKMFTVLNTQYTFDENYMRCVSEHMKELKPFGDVPDKLSVQIKRSFVATRTYGQALTTASEVAKKVLNVRLNADCTGALTKMQHCGACKGYTEKPCTNYCVNVIKGCLHYQHEFDSEWENFAMAMDKVAERLLGSFNIVMVVEPLNIKISEAIMNFQDSGQDITNRVFQGCGRPKLKKMKRSISPKLQGVQILNARSPVEADTLDIDETLDEAIVLRERRAAEPGSQETSAQQSQEQGVGKSGNGGGGGGGNNRRQQQRRKQQQQRRKQQNNRDDNDDDDNESGGGREPILDRIVRDIRQRVKDYKKFWSNLPHSVCSNEDIASSSDVDGMCWNGHTIDRYMHSITTEHGSNPEFTGNPASTKQTAQMASQLSHLKNAIVHLRNAYNGQDVEWSEQEELPYAGSGAGSGSGSEDDEDDDEGSGLGPFEPSHKPDVERPSVDADNDDDEDAGGRGHMPTHTSRPTSGVDDKNPLIHTTHFDQDHNDLDEDHRQLDEDEDTDAGHDGANDNRSSDAPEKMTLRRALVVYLLPLYMAWFGGVCADLL

Target ORF information:

RefSeq Version	NM_079347.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dally-like (dlp), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079347.3

ORF Insert Sequence:

ATGCTACATC AGCAGCAACA ACAACAACAT CTGCATTGCA GAAGAAAAGC TACAGCAACA 
ACAACTGCCC GCCTTGTGAT ATTCAGCAGT CCTCTTCTAC TATTACTACT CACCACCCAC 
TTGCCGCCCA CCTTACAAGC GGACAATGGC CCCGCCCCCC AAGTGGCCGC CCTTGCCGCC 
CCCAATCCGG CGGGTGGCGT AGCAGGGTCT TCGATCATCG ATCAATTCAG TCCGAATTGC 
AGTGCTGTGA CACACATTTT CCAAGCGAGA GGAATCGATG CCATCGAAAT TCCCCAAAAA 
CCCAGTAACG AACGAGTCCT ACGATATTGC GAGTCCCCTT CGGTGGGCAC TTGTTGCACC 
TACAACATGG AGACCCGCAT GGCGATGCAA TCCCGCCAGC AGCTCGAAGG GCACACCAAG 
GACCAGATCT CTCGTATGTC TGGAATCCTG GGCAGCAAGG CCACAAAATT CAAAGATATT 
TTCACGGCCC TGCTCAAAGA GTCGCGAACG CAGTTCAATA GTATGTTCAT CCGAACATAC 
GGCGTCATTT ACGAGAGAAA TTCGTACGTC TTCTCGGATC TGTTCAAGGA GTTGGAAACC 
TACTTCGCCA ACGGTCGCGT GGACTTGCTG GAGGTCATGG ACAAGTTCTT CAATACGCTA 
TACCAGAAGA TGTTCACCGT GCTGAACACC CAGTACACAT TTGATGAAAA CTACATGCGA 
TGCGTCAGCG AGCACATGAA GGAGCTGAAG CCTTTCGGAG ATGTTCCGGA CAAGCTGTCC 
GTGCAAATCA AGCGATCCTT TGTGGCCACG CGCACCTATG GCCAGGCTCT GACCACCGCT 
TCGGAGGTGG CGAAGAAGGT CCTCAATGTG CGACTGAATG CCGATTGTAC TGGGGCTCTT 
ACCAAGATGC AGCACTGTGG TGCTTGCAAG GGATATACGG AAAAGCCCTG TACCAACTAC 
TGTGTGAACG TCATCAAGGG ATGCCTGCAC TATCAGCACG AGTTCGACAG CGAATGGGAG 
AACTTTGCGA TGGCCATGGA CAAGGTGGCG GAACGACTGC TGGGATCCTT CAACATTGTG 
ATGGTGGTGG AGCCACTCAA CATCAAGATC TCCGAGGCCA TCATGAACTT CCAGGATTCT 
GGCCAGGACA TCACCAACCG CGTATTCCAA GGATGCGGTA GGCCAAAGTT GAAGAAAATG 
AAACGGTCCA TCAGCCCCAA ACTGCAAGGA GTTCAAATCC TCAACGCAAG GAGTCCTGTG 
GAGGCCGATA CTCTGGACAT TGATGAAACG TTGGACGAGG CGATTGTGCT GAGAGAACGC 
AGAGCTGCTG AGCCCGGCTC ACAGGAGACA TCGGCGCAAC AGTCTCAAGA GCAAGGAGTT 
GGAAAGAGTG GAAATGGAGG AGGAGGCGGT GGCGGTAACA ACAGGCGGCA ACAGCAACGT 
CGAAAGCAGC AGCAACAGCG ACGCAAACAG CAAAACAATC GCGACGACAA CGATGACGAT 
GACAATGAGA GCGGAGGTGG ACGTGAACCG ATTCTGGACA GGATCGTGAG GGACATTCGC 
CAGCGAGTGA AGGACTACAA AAAGTTCTGG TCCAATCTGC CGCACTCGGT TTGTAGCAAC 
GAGGATATAG CCTCCAGTTC CGATGTGGAC GGCATGTGCT GGAATGGTCA CACAATTGAT 
AGATACATGC ACTCAATCAC CACAGAACAC GGCTCGAATC CGGAGTTTAC AGGCAATCCT 
GCCAGTACTA AGCAAACAGC TCAAATGGCC TCTCAACTAA GTCATCTGAA GAATGCCATT 
GTCCACCTTC GAAATGCCTA CAACGGCCAG GATGTTGAGT GGAGCGAACA AGAAGAACTG 
CCATATGCGG GTAGTGGAGC TGGTTCTGGA TCCGGGTCCG AAGACGACGA GGACGATGAC 
GAGGGCTCTG GCCTGGGACC CTTCGAACCC AGCCATAAGC CGGACGTGGA GCGTCCTTCG 
GTGGATGCAG ACAACGACGA TGACGAGGAC GCCGGTGGAC GGGGTCACAT GCCCACGCAC 
ACATCTCGAC CGACGAGCGG AGTGGACGAC AAGAATCCGC TCATCCACAC CACTCACTTC 
GACCAAGATC ACAACGATCT GGATGAGGAT CATAGGCAGC TGGATGAGGA TGAGGACACG 
GATGCAGGCC ATGACGGGGC CAATGACAAT CGATCATCCG ATGCCCCAGA AAAGATGACG 
CTGCGTCGCG CTCTGGTTGT TTATCTGCTG CCCCTTTATA TGGCGTGGTT CGGTGGCGTC 
TGCGCCGATT TGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dlp ( NM_206353.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996075.2 Drosophila melanogaster dally-like (dlp), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa20193
Clone ID Related Accession (Same CDS sequence)	NM_206353.2
Accession Version	NM_206353.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dally-like, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_206353.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTACATC AGCAGCAACA ACAACAACAT CTGCATTGCA GAAGAAAAGC TACAGCAACA 
ACAACTGCCC GCCTTGTGAT ATTCAGCAGT CCTCTTCTAC TATTACTACT CACCACCCAC 
TTGCCGCCCA CCTTACAAGC GGACAATGGC CCCGCCCCCC AAGTGGCCGC CCTTGCCGCC 
CCCAATCCGG CGGGTGGCGT AGCAGGGTCT TCGATCATCG ATCAATTCAG TCCGAATTGC 
AGTGCTGTGA CACACATTTT CCAAGCGAGA GGAATCGATG CCATCGAAAT TCCCCAAAAA 
CCCAGTAACG AACGAGTCCT ACGATATTGC GAGTCCCCTT CGGTGGGCAC TTGTTGCACC 
TACAACATGG AGACCCGCAT GGCGATGCAA TCCCGCCAGC AGCTCGAAGG GCACACCAAG 
GACCAGATCT CTCGTATGTC TGGAATCCTG GGCAGCAAGG CCACAAAATT CAAAGATATT 
TTCACGGCCC TGCTCAAAGA GTCGCGAACG CAGTTCAATA GTATGTTCAT CCGAACATAC 
GGCGTCATTT ACGAGAGAAA TTCGTACGTC TTCTCGGATC TGTTCAAGGA GTTGGAAACC 
TACTTCGCCA ACGGTCGCGT GGACTTGCTG GAGGTCATGG ACAAGTTCTT CAATACGCTA 
TACCAGAAGA TGTTCACCGT GCTGAACACC CAGTACACAT TTGATGAAAA CTACATGCGA 
TGCGTCAGCG AGCACATGAA GGAGCTGAAG CCTTTCGGAG ATGTTCCGGA CAAGCTGTCC 
GTGCAAATCA AGCGATCCTT TGTGGCCACG CGCACCTATG GCCAGGCTCT GACCACCGCT 
TCGGAGGTGG CGAAGAAGGT CCTCAATGTG CGACTGAATG CCGATTGTAC TGGGGCTCTT 
ACCAAGATGC AGCACTGTGG TGCTTGCAAG GGATATACGG AAAAGCCCTG TACCAACTAC 
TGTGTGAACG TCATCAAGGG ATGCCTGCAC TATCAGCACG AGTTCGACAG CGAATGGGAG 
AACTTTGCGA TGGCCATGGA CAAGGTGGCG GAACGACTGC TGGGATCCTT CAACATTGTG 
ATGGTGGTGG AGCCACTCAA CATCAAGATC TCCGAGGCCA TCATGAACTT CCAGGATTCT 
GGCCAGGACA TCACCAACCG CGTATTCCAA GGATGCGGTA GGCCAAAGTT GAAGAAAATG 
AAACGGTCCA TCAGCCCCAA ACTGCAAGGA GTTCAAATCC TCAACGCAAG GAGTCCTGTG 
GAGGCCGATA CTCTGGACAT TGATGAAACG TTGGACGAGG CGATTGTGCT GAGAGAACGC 
AGAGCTGCTG AGCCCGGCTC ACAGGAGACA TCGGCGCAAC AGTCTCAAGA GCAAGGAGTT 
GGAAAGAGTG GAAATGGAGG AGGAGGCGGT GGCGGTAACA ACAGGCGGCA ACAGCAACGT 
CGAAAGCAGC AGCAACAGCG ACGCAAACAG CAAAACAATC GCGACGACAA CGATGACGAT 
GACAATGAGA GCGGAGGTGG ACGTGAACCG ATTCTGGACA GGATCGTGAG GGACATTCGC 
CAGCGAGTGA AGGACTACAA AAAGTTCTGG TCCAATCTGC CGCACTCGGT TTGTAGCAAC 
GAGGATATAG CCTCCAGTTC CGATGTGGAC GGCATGTGCT GGAATGGTCA CACAATTGAT 
AGATACATGC ACTCAATCAC CACAGAACAC GGCTCGAATC CGGAGTTTAC AGGCAATCCT 
GCCAGTACTA AGCAAACAGC TCAAATGGCC TCTCAACTAA GTCATCTGAA GAATGCCATT 
GTCCACCTTC GAAATGCCTA CAACGGCCAG GATGTTGAGT GGAGCGAACA AGAACTGCCA 
TATGCGGGTA GTGGAGCTGG TTCTGGATCC GGGTCCGAAG ACGACGAGGA CGATGACGAG 
GGCTCTGGCC TGGGACCCTT CGAACCCAGC CATAAGCCGG ACGTGGAGCG TCCTTCGGTG 
GATGCAGACA ACGACGATGA CGAGGACGCC GGTGGACGGG GTCACATGCC CACGCACACA 
TCTCGACCGA CGAGCGGAGT GGACGACAAG AATCCGCTCA TCCACACCAC TCACTTCGAC 
CAAGATCACA ACGATCTGGA TGAGGATCAT AGGCAGCTGG ATGAGGATGA GGACACGGAT 
GCAGGCCATG ACGGGGCCAA TGACAATCGA TCATCCGATG CCCCAGAAAA GATGACGCTG 
CGTCGCGCTC TGGTTGTTTA TCTGCTGCCC CTTTATATGG CGTGGTTCGG TGGCGTCTGC 
GCCGATTTGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996075.2
CDS	473..2767
Translation	MLHQQQQQQHLHCRRKATATTTARLVIFSSPLLLLLLTTHLPPTLQADNGPAPQVAALAAPNPAGGVAGSSIIDQFSPNCSAVTHIFQARGIDAIEIPQKPSNERVLRYCESPSVGTCCTYNMETRMAMQSRQQLEGHTKDQISRMSGILGSKATKFKDIFTALLKESRTQFNSMFIRTYGVIYERNSYVFSDLFKELETYFANGRVDLLEVMDKFFNTLYQKMFTVLNTQYTFDENYMRCVSEHMKELKPFGDVPDKLSVQIKRSFVATRTYGQALTTASEVAKKVLNVRLNADCTGALTKMQHCGACKGYTEKPCTNYCVNVIKGCLHYQHEFDSEWENFAMAMDKVAERLLGSFNIVMVVEPLNIKISEAIMNFQDSGQDITNRVFQGCGRPKLKKMKRSISPKLQGVQILNARSPVEADTLDIDETLDEAIVLRERRAAEPGSQETSAQQSQEQGVGKSGNGGGGGGGNNRRQQQRRKQQQQRRKQQNNRDDNDDDDNESGGGREPILDRIVRDIRQRVKDYKKFWSNLPHSVCSNEDIASSSDVDGMCWNGHTIDRYMHSITTEHGSNPEFTGNPASTKQTAQMASQLSHLKNAIVHLRNAYNGQDVEWSEQELPYAGSGAGSGSGSEDDEDDDEGSGLGPFEPSHKPDVERPSVDADNDDDEDAGGRGHMPTHTSRPTSGVDDKNPLIHTTHFDQDHNDLDEDHRQLDEDEDTDAGHDGANDNRSSDAPEKMTLRRALVVYLLPLYMAWFGGVCADLL

Target ORF information:

RefSeq Version	NM_206353.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dally-like (dlp), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206353.2

ORF Insert Sequence:

ATGCTACATC AGCAGCAACA ACAACAACAT CTGCATTGCA GAAGAAAAGC TACAGCAACA 
ACAACTGCCC GCCTTGTGAT ATTCAGCAGT CCTCTTCTAC TATTACTACT CACCACCCAC 
TTGCCGCCCA CCTTACAAGC GGACAATGGC CCCGCCCCCC AAGTGGCCGC CCTTGCCGCC 
CCCAATCCGG CGGGTGGCGT AGCAGGGTCT TCGATCATCG ATCAATTCAG TCCGAATTGC 
AGTGCTGTGA CACACATTTT CCAAGCGAGA GGAATCGATG CCATCGAAAT TCCCCAAAAA 
CCCAGTAACG AACGAGTCCT ACGATATTGC GAGTCCCCTT CGGTGGGCAC TTGTTGCACC 
TACAACATGG AGACCCGCAT GGCGATGCAA TCCCGCCAGC AGCTCGAAGG GCACACCAAG 
GACCAGATCT CTCGTATGTC TGGAATCCTG GGCAGCAAGG CCACAAAATT CAAAGATATT 
TTCACGGCCC TGCTCAAAGA GTCGCGAACG CAGTTCAATA GTATGTTCAT CCGAACATAC 
GGCGTCATTT ACGAGAGAAA TTCGTACGTC TTCTCGGATC TGTTCAAGGA GTTGGAAACC 
TACTTCGCCA ACGGTCGCGT GGACTTGCTG GAGGTCATGG ACAAGTTCTT CAATACGCTA 
TACCAGAAGA TGTTCACCGT GCTGAACACC CAGTACACAT TTGATGAAAA CTACATGCGA 
TGCGTCAGCG AGCACATGAA GGAGCTGAAG CCTTTCGGAG ATGTTCCGGA CAAGCTGTCC 
GTGCAAATCA AGCGATCCTT TGTGGCCACG CGCACCTATG GCCAGGCTCT GACCACCGCT 
TCGGAGGTGG CGAAGAAGGT CCTCAATGTG CGACTGAATG CCGATTGTAC TGGGGCTCTT 
ACCAAGATGC AGCACTGTGG TGCTTGCAAG GGATATACGG AAAAGCCCTG TACCAACTAC 
TGTGTGAACG TCATCAAGGG ATGCCTGCAC TATCAGCACG AGTTCGACAG CGAATGGGAG 
AACTTTGCGA TGGCCATGGA CAAGGTGGCG GAACGACTGC TGGGATCCTT CAACATTGTG 
ATGGTGGTGG AGCCACTCAA CATCAAGATC TCCGAGGCCA TCATGAACTT CCAGGATTCT 
GGCCAGGACA TCACCAACCG CGTATTCCAA GGATGCGGTA GGCCAAAGTT GAAGAAAATG 
AAACGGTCCA TCAGCCCCAA ACTGCAAGGA GTTCAAATCC TCAACGCAAG GAGTCCTGTG 
GAGGCCGATA CTCTGGACAT TGATGAAACG TTGGACGAGG CGATTGTGCT GAGAGAACGC 
AGAGCTGCTG AGCCCGGCTC ACAGGAGACA TCGGCGCAAC AGTCTCAAGA GCAAGGAGTT 
GGAAAGAGTG GAAATGGAGG AGGAGGCGGT GGCGGTAACA ACAGGCGGCA ACAGCAACGT 
CGAAAGCAGC AGCAACAGCG ACGCAAACAG CAAAACAATC GCGACGACAA CGATGACGAT 
GACAATGAGA GCGGAGGTGG ACGTGAACCG ATTCTGGACA GGATCGTGAG GGACATTCGC 
CAGCGAGTGA AGGACTACAA AAAGTTCTGG TCCAATCTGC CGCACTCGGT TTGTAGCAAC 
GAGGATATAG CCTCCAGTTC CGATGTGGAC GGCATGTGCT GGAATGGTCA CACAATTGAT 
AGATACATGC ACTCAATCAC CACAGAACAC GGCTCGAATC CGGAGTTTAC AGGCAATCCT 
GCCAGTACTA AGCAAACAGC TCAAATGGCC TCTCAACTAA GTCATCTGAA GAATGCCATT 
GTCCACCTTC GAAATGCCTA CAACGGCCAG GATGTTGAGT GGAGCGAACA AGAACTGCCA 
TATGCGGGTA GTGGAGCTGG TTCTGGATCC GGGTCCGAAG ACGACGAGGA CGATGACGAG 
GGCTCTGGCC TGGGACCCTT CGAACCCAGC CATAAGCCGG ACGTGGAGCG TCCTTCGGTG 
GATGCAGACA ACGACGATGA CGAGGACGCC GGTGGACGGG GTCACATGCC CACGCACACA 
TCTCGACCGA CGAGCGGAGT GGACGACAAG AATCCGCTCA TCCACACCAC TCACTTCGAC 
CAAGATCACA ACGATCTGGA TGAGGATCAT AGGCAGCTGG ATGAGGATGA GGACACGGAT 
GCAGGCCATG ACGGGGCCAA TGACAATCGA TCATCCGATG CCCCAGAAAA GATGACGCTG 
CGTCGCGCTC TGGTTGTTTA TCTGCTGCCC CTTTATATGG CGTGGTTCGG TGGCGTCTGC 
GCCGATTTGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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dlp cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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