btl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	btl
Entrez Gene ID	39564
Full Name	breathless
Synonyms	0844/01,BTL/FGFR2,Btl,CG32134,CG6714,CT20816,D-FGFR,DFGF-R1,DFR2,Dfr-2,DmHD-311,Dmel\CG32134,Dtk2,FGFR,FGFR1,Fgf-r,HD-311,Tk2,dFGFR,dev,dtk2,fgf-r,l(3)00208,lambdatop
General protein information	Preferred Names breathless Names CG32134 gene product from transcript CG32134-RA CG32134-PA CG32134-PB FGF receptor FGFR Fibroblast-growth-factor-receptor btl-PA btl-PB devenir fibrobast growth factor receptor fibroblast growth factor receptor fibroblast growth factor receptor 1 no terminal cell clones-L
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 70D2-70D2\|3-40.9 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_168577.3	NP_729956.1	breathless, isoform A
NM_001014583.2	NP_001014583.1	breathless, isoform B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	FGFR2	NP_001003336.1
Mus musculus (house mouse)	Fgfr2	NP_034337.2
Bos taurus (cattle)	FGFR2	NP_001192239.1
Gallus gallus (chicken)	FGFR2	NP_990650.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003108	XP_562866.4
Homo sapiens (human)	FGFR2	NP_075259.4
Pan troglodytes (chimpanzee)	FGFR2	XP_001157390.2
Danio rerio (zebrafish)	fgfr2	NP_001229933.1
Rattus norvegicus (Norway rat)	Fgfr2	NP_001103362.1
Drosophila melanogaster (fruit fly)	btl	NP_001014583.1
Xenopus tropicalis (tropical clawed frog)	fgfr2	NP_001096326.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Time lapse imaging shows dynamic reorganization of branchless (bnl)-expressing cells and bnl-receptor breathless (btl) expressing trachea during embryonic development.
Title: Unique patterns of organization and migration of FGF-expressing cells during Drosophila morphogenesis.

Transgene expression of Drosophila melanogaster nucleoside kinase reverses mitochondrial thymidine kinase 2 deficiency
Title: Transgene expression of Drosophila melanogaster nucleoside kinase reverses mitochondrial thymidine kinase 2 deficiency.

Study demonstrates that jing is required for btl expression in the branching trachea and dominantly interacts with known regulators of btl expression, including the ETS and POU transcription factors, pointed, and drifter/ventral veinless, respectively
Title: Drosophila Jing is part of the breathless fibroblast growth factor receptor positive feedback loop.

Data show that Downstream-of-FGFR is a substrate for fibroblast growth factor receptors (FGFRs) and recruits Corkscrew, leading to FGF-induced cell migration and activation of the Ras/MAPK pathway.
Title: Downstream-of-FGFR is a fibroblast growth factor-specific scaffolding protein and recruits Corkscrew upon receptor activation.

In embryos, Fgfr1c was detected in osteoblasts of calvarial and mandibular bones. Fgfr3b and Fgfr3c were in chondrocytes in cranial base and mandibular condyle. Fgfr2b transcripts were detected in perichondria of mandibular condyle and cranial base.
Title: Fgfr mRNA isoforms in craniofacial bone development.

The following btl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the btl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18478	NM_001014583.2 Latest version!	Drosophila melanogaster breathless (btl), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa18478	NM_168577.3 Latest version!	Drosophila melanogaster breathless (btl), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

btl ( NM_168577.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729956.1 Drosophila melanogaster breathless (btl), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18478
Clone ID Related Accession (Same CDS sequence)	NM_168577.3 , NM_001014583.2
Accession Version	NM_168577.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	breathless, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168577.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAAAAG TGCCGATCAC GCTGGTGATG ATCATTGCGA TCGTCTCTGC CGCTGCGGAC 
CTGGGATGCG ATTATGGCCA TCATCGTTGC TACATCGATG TGACTGTGGA AAACAGTCCC 
CGCCAGCGCC ATCTGCTCTC CGACATGGAC ATCACGCTGC AGTGCGTCCG ACCCATGGCC 
AAATGGTTCT ATGAGGATAA GTTTCAACTG AGGGCTACCC TTTTGCGACT TGAACGAGCA 
CAGTCCGGAA ATTCTGGAAA CTACGGCTGC CTGGATAGTC AGAACAGATG GTACAATATT 
TCCCTTGTTG TTGGCCATAA GGAACCAGTT GGCAATGATA TCGCCAGCTT TGTCAAGTTG 
GAAGATGCAC CGGCCCTCCC GGAGTCAGAT CTCTTTTTTC AGCCCCTGAA TGAGAGTCGA 
TCCCTGAAGT TGCTGCAACC CCTACCCAAA ACCGTACAAC GGACCGCAGG TGGCCTTTTT 
CAGTTGAACT GCAGTCCCAT GGATCCGGAT GCAAAGGGTG TTAATATATC CTGGCTGCAC 
AATGATACTC AAATCCTTGG TGGACGTGGA CGCATTAAGC TTAAAAGGTG GTCATTAACA 
GTGGGGCAAC TGCAGCCGGA GGATGCTGGA AGCTATCACT GTGAGCTGTG CGTGGAACAG 
GACTGCCAAA GGAGTAATCC GACCCAATTG GAGGTGATAA GCAGAAAGCA CACGGTGCCC 
ATGCTAAAGC CCGGATATCC CCGCAATACC AGCATTGCCC TCGGGGACAA TGTGAGCATT 
GAATGCCTAC TTGAGGACTC TGCCCTGGAG CCGAAGATCA CGTGGCTACA TAAGGGGAAT 
GCGGACAACA TCGATGACCT CTTGCAGCGT TTAAGAGAGC AGTCCCAGCT ACCTGTGGAT 
GTTACCCGAT TGATTACCCG AATGGATGAA CCGCAGGTAC TTCGTCTTGG AAATGTCCTG 
ATGGAGGATG GCGGATGGTA CATATGCATA GCCGAGAACC AGGTTGGACG CACTGTGGCC 
GCCTCCTATG TGGATCTTTA CAGTCCCTCA GATACTACCA CAGTTCGCAC GACCACTACT 
ACCACCGTGG CCAGCCCAAT ACCCACTGCA TCCACCGGAG AGGACAACGA TGACGACGTG 
GAAAATCCAG CAGCGGAAGC ATCAGGCGGC GTTGGTCCAC CTGTGTTCAG AAAAGAGCTG 
AAACGATTAC AGCACTCATT ATCGGGAAAT ACTGTCAATC TAGCCTGTCC AGTGTACGGC 
AAAGCGAACA TCACCTGGAC GAAGGACAAG AAGCCGCTAA ACCGTGAACT CGGCGTCTAT 
GTGCAAAAGA ACTGGACACT CCGCTTCGTG GAGGCCACCA GCGAGGATTC TGGACTATAC 
AACTGTAAGG TGTGCAATGC GTGGGGCTGC ATCCAGTTTG ACTTCAGTGT CCAAATAAAC 
GATCGCACGA GATCCGCACC AATAATCGTG GTGCCGCAAA ATCAGACGGT GAAAGTCAAT 
GGCAGCTTGG TGATGAAGTG CACCGTCTAC TCTGATCTGC ATCCAACAGT CAGCTGGAAA 
AGGGTTGTCC TCAAGAATGC CTCCTTGGAC GGCCTTAAGT CTGTGGAAAT CCAGAATCTC 
AACTTCACCG TGACGAATGA CTCCGTAGTT TTAACGCTTC GTAATGTGAC CTTTGACCAG 
GAGGGCTGGT ACACCTGTTT GGCCTCAAGT GGTCTGGGAA GGAGTAACTC CAGTGTGTAC 
CTTAGGGTAG TGAGTCCTCT GCCACCTTTG GAGATCTACG CCCTGCTGCA CGCCCATCCT 
CTCGGATTCA CCTTGGCCGC TATTACGATA GTGGCTTTGT TCCTTTTGGG CAGTGCTTTC 
ATAACGTTCA TGCTGCGTCG GCTGAGGAGG GAGAAGCTCC TGAAGCTCCG CATCGAAACG 
GTTCATCAGT GGACTAAAAA GGTGATCATA TACCGACCGG GTGGAGAAGA AGGAAGTGGC 
TGCAGCTCCG GCGATCTTCA GATGCCAGTG ATAAGGATCG AGAAGCAGAG AACAACCGTT 
TCCACAACGG GAACTGGCGG TACTGATCCC GCCCAGGGCT TTAATGAGTA TGAGTTCCCG 
CTGGACTCAA ACTGGGAGAT TCCCAGGCAG CAGCTTAGCT TGGGCTCCAT TTTGGGTGAG 
GGCGCCTTTG GACGCGTAGT GATGGCGGAG GCGGAGGGGC TTCCCAGGAG TCCACAACTG 
GCCGAAACCA TCGTGGCCGT CAAGATGGTC AAGGAGGAGC ACACGGATAC GGACATGGCC 
AGTTTGGTGC GTGAAATGGA GGTGATGAAA ATGATTGGCA AGCACATAAA CATCATCAAT 
CTGCTAGGCT GCTGCAGCCA GGGCGGTCCT CTGTGGGTGA TCGTGGAGTA TGCTCCACAC 
GGAAACCTCA AGGACTTCCT CAAGCAGAAT CGACCTGGTG CTCCACAGCG ACGGAGTGAC 
AGTGATGGCT ATCTAGATGA CAAGCCACTG ATTTCCACTC AACATCTTGG CGAAAAGGAG 
CTCACCAAGT TTGCATTTCA AATCGCTCGA GGAATGGAGT ACCTTGCTTC AAGACGGTGC 
ATTCATCGCG ATCTGGCTGC CCGTAATGTC CTTGTAAGCG ATGGATACGT TATGAAGATC 
GCCGATTTTG GTCTGGCGAG GGATATCCAA GATACGGAGT ACTACCGAAA AAATACCAAT 
GGACGGTTGC CCATTAAGTG GATGGCGCCC GAGTCGCTGC AGGAGAAGAA GTACGACTCA 
CAGAGCGACG TCTGGAGCTA TGGTGTTCTG CTGTGGGAGA TCATGACCTA CGGGGATCAA 
CCATACCCGC ACATACTGTC TGCCGAGGAG CTCTACAGCT ACCTGATTAC GGGTCAGCGC 
ATGGAGAAAC CGGCCAAGTG CTCCCTGAAC ATCTACGTGG TAATGCGACA GTGCTGGCAC 
TTTGAGTCCT GTGCACGACC CACGTTCGCG GAGCTGGTAG AGAGTTTCGA TGGGATCCTG 
CAGCAGGCTA GTAGCAATCC AAACGATGCC TATCTGGACC TTTCGATGCC GATGCTGGAG 
ACGCCGCCCT CATCGGGGGA TGAGGATGAT GGCTCCGACA CCGAAACATT CCGGGAAACG 
TCCCCGCTTA GATATCAGTA CACCTATAAG TTTAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729956.1
CDS	373..3531
Translation	MAKVPITLVMIIAIVSAAADLGCDYGHHRCYIDVTVENSPRQRHLLSDMDITLQCVRPMAKWFYEDKFQLRATLLRLERAQSGNSGNYGCLDSQNRWYNISLVVGHKEPVGNDIASFVKLEDAPALPESDLFFQPLNESRSLKLLQPLPKTVQRTAGGLFQLNCSPMDPDAKGVNISWLHNDTQILGGRGRIKLKRWSLTVGQLQPEDAGSYHCELCVEQDCQRSNPTQLEVISRKHTVPMLKPGYPRNTSIALGDNVSIECLLEDSALEPKITWLHKGNADNIDDLLQRLREQSQLPVDVTRLITRMDEPQVLRLGNVLMEDGGWYICIAENQVGRTVAASYVDLYSPSDTTTVRTTTTTTVASPIPTASTGEDNDDDVENPAAEASGGVGPPVFRKELKRLQHSLSGNTVNLACPVYGKANITWTKDKKPLNRELGVYVQKNWTLRFVEATSEDSGLYNCKVCNAWGCIQFDFSVQINDRTRSAPIIVVPQNQTVKVNGSLVMKCTVYSDLHPTVSWKRVVLKNASLDGLKSVEIQNLNFTVTNDSVVLTLRNVTFDQEGWYTCLASSGLGRSNSSVYLRVVSPLPPLEIYALLHAHPLGFTLAAITIVALFLLGSAFITFMLRRLRREKLLKLRIETVHQWTKKVIIYRPGGEEGSGCSSGDLQMPVIRIEKQRTTVSTTGTGGTDPAQGFNEYEFPLDSNWEIPRQQLSLGSILGEGAFGRVVMAEAEGLPRSPQLAETIVAVKMVKEEHTDTDMASLVREMEVMKMIGKHINIINLLGCCSQGGPLWVIVEYAPHGNLKDFLKQNRPGAPQRRSDSDGYLDDKPLISTQHLGEKELTKFAFQIARGMEYLASRRCIHRDLAARNVLVSDGYVMKIADFGLARDIQDTEYYRKNTNGRLPIKWMAPESLQEKKYDSQSDVWSYGVLLWEIMTYGDQPYPHILSAEELYSYLITGQRMEKPAKCSLNIYVVMRQCWHFESCARPTFAELVESFDGILQQASSNPNDAYLDLSMPMLETPPSSGDEDDGSDTETFRETSPLRYQYTYKFN

Target ORF information:

RefSeq Version	NM_168577.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster breathless (btl), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168577.3

ORF Insert Sequence:

ATGGCAAAAG TGCCGATCAC GCTGGTGATG ATCATTGCGA TCGTCTCTGC CGCTGCGGAC 
CTGGGATGCG ATTATGGCCA TCATCGTTGC TACATCGATG TGACTGTGGA AAACAGTCCC 
CGCCAGCGCC ATCTGCTCTC CGACATGGAC ATCACGCTGC AGTGCGTCCG ACCCATGGCC 
AAATGGTTCT ATGAGGATAA GTTTCAACTG AGGGCTACCC TTTTGCGACT TGAACGAGCA 
CAGTCCGGAA ATTCTGGAAA CTACGGCTGC CTGGATAGTC AGAACAGATG GTACAATATT 
TCCCTTGTTG TTGGCCATAA GGAACCAGTT GGCAATGATA TCGCCAGCTT TGTCAAGTTG 
GAAGATGCAC CGGCCCTCCC GGAGTCAGAT CTCTTTTTTC AGCCCCTGAA TGAGAGTCGA 
TCCCTGAAGT TGCTGCAACC CCTACCCAAA ACCGTACAAC GGACCGCAGG TGGCCTTTTT 
CAGTTGAACT GCAGTCCCAT GGATCCGGAT GCAAAGGGTG TTAATATATC CTGGCTGCAC 
AATGATACTC AAATCCTTGG TGGACGTGGA CGCATTAAGC TTAAAAGGTG GTCATTAACA 
GTGGGGCAAC TGCAGCCGGA GGATGCTGGA AGCTATCACT GTGAGCTGTG CGTGGAACAG 
GACTGCCAAA GGAGTAATCC GACCCAATTG GAGGTGATAA GCAGAAAGCA CACGGTGCCC 
ATGCTAAAGC CCGGATATCC CCGCAATACC AGCATTGCCC TCGGGGACAA TGTGAGCATT 
GAATGCCTAC TTGAGGACTC TGCCCTGGAG CCGAAGATCA CGTGGCTACA TAAGGGGAAT 
GCGGACAACA TCGATGACCT CTTGCAGCGT TTAAGAGAGC AGTCCCAGCT ACCTGTGGAT 
GTTACCCGAT TGATTACCCG AATGGATGAA CCGCAGGTAC TTCGTCTTGG AAATGTCCTG 
ATGGAGGATG GCGGATGGTA CATATGCATA GCCGAGAACC AGGTTGGACG CACTGTGGCC 
GCCTCCTATG TGGATCTTTA CAGTCCCTCA GATACTACCA CAGTTCGCAC GACCACTACT 
ACCACCGTGG CCAGCCCAAT ACCCACTGCA TCCACCGGAG AGGACAACGA TGACGACGTG 
GAAAATCCAG CAGCGGAAGC ATCAGGCGGC GTTGGTCCAC CTGTGTTCAG AAAAGAGCTG 
AAACGATTAC AGCACTCATT ATCGGGAAAT ACTGTCAATC TAGCCTGTCC AGTGTACGGC 
AAAGCGAACA TCACCTGGAC GAAGGACAAG AAGCCGCTAA ACCGTGAACT CGGCGTCTAT 
GTGCAAAAGA ACTGGACACT CCGCTTCGTG GAGGCCACCA GCGAGGATTC TGGACTATAC 
AACTGTAAGG TGTGCAATGC GTGGGGCTGC ATCCAGTTTG ACTTCAGTGT CCAAATAAAC 
GATCGCACGA GATCCGCACC AATAATCGTG GTGCCGCAAA ATCAGACGGT GAAAGTCAAT 
GGCAGCTTGG TGATGAAGTG CACCGTCTAC TCTGATCTGC ATCCAACAGT CAGCTGGAAA 
AGGGTTGTCC TCAAGAATGC CTCCTTGGAC GGCCTTAAGT CTGTGGAAAT CCAGAATCTC 
AACTTCACCG TGACGAATGA CTCCGTAGTT TTAACGCTTC GTAATGTGAC CTTTGACCAG 
GAGGGCTGGT ACACCTGTTT GGCCTCAAGT GGTCTGGGAA GGAGTAACTC CAGTGTGTAC 
CTTAGGGTAG TGAGTCCTCT GCCACCTTTG GAGATCTACG CCCTGCTGCA CGCCCATCCT 
CTCGGATTCA CCTTGGCCGC TATTACGATA GTGGCTTTGT TCCTTTTGGG CAGTGCTTTC 
ATAACGTTCA TGCTGCGTCG GCTGAGGAGG GAGAAGCTCC TGAAGCTCCG CATCGAAACG 
GTTCATCAGT GGACTAAAAA GGTGATCATA TACCGACCGG GTGGAGAAGA AGGAAGTGGC 
TGCAGCTCCG GCGATCTTCA GATGCCAGTG ATAAGGATCG AGAAGCAGAG AACAACCGTT 
TCCACAACGG GAACTGGCGG TACTGATCCC GCCCAGGGCT TTAATGAGTA TGAGTTCCCG 
CTGGACTCAA ACTGGGAGAT TCCCAGGCAG CAGCTTAGCT TGGGCTCCAT TTTGGGTGAG 
GGCGCCTTTG GACGCGTAGT GATGGCGGAG GCGGAGGGGC TTCCCAGGAG TCCACAACTG 
GCCGAAACCA TCGTGGCCGT CAAGATGGTC AAGGAGGAGC ACACGGATAC GGACATGGCC 
AGTTTGGTGC GTGAAATGGA GGTGATGAAA ATGATTGGCA AGCACATAAA CATCATCAAT 
CTGCTAGGCT GCTGCAGCCA GGGCGGTCCT CTGTGGGTGA TCGTGGAGTA TGCTCCACAC 
GGAAACCTCA AGGACTTCCT CAAGCAGAAT CGACCTGGTG CTCCACAGCG ACGGAGTGAC 
AGTGATGGCT ATCTAGATGA CAAGCCACTG ATTTCCACTC AACATCTTGG CGAAAAGGAG 
CTCACCAAGT TTGCATTTCA AATCGCTCGA GGAATGGAGT ACCTTGCTTC AAGACGGTGC 
ATTCATCGCG ATCTGGCTGC CCGTAATGTC CTTGTAAGCG ATGGATACGT TATGAAGATC 
GCCGATTTTG GTCTGGCGAG GGATATCCAA GATACGGAGT ACTACCGAAA AAATACCAAT 
GGACGGTTGC CCATTAAGTG GATGGCGCCC GAGTCGCTGC AGGAGAAGAA GTACGACTCA 
CAGAGCGACG TCTGGAGCTA TGGTGTTCTG CTGTGGGAGA TCATGACCTA CGGGGATCAA 
CCATACCCGC ACATACTGTC TGCCGAGGAG CTCTACAGCT ACCTGATTAC GGGTCAGCGC 
ATGGAGAAAC CGGCCAAGTG CTCCCTGAAC ATCTACGTGG TAATGCGACA GTGCTGGCAC 
TTTGAGTCCT GTGCACGACC CACGTTCGCG GAGCTGGTAG AGAGTTTCGA TGGGATCCTG 
CAGCAGGCTA GTAGCAATCC AAACGATGCC TATCTGGACC TTTCGATGCC GATGCTGGAG 
ACGCCGCCCT CATCGGGGGA TGAGGATGAT GGCTCCGACA CCGAAACATT CCGGGAAACG 
TCCCCGCTTA GATATCAGTA CACCTATAAG TTTAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

btl ( NM_001014583.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014583.1 Drosophila melanogaster breathless (btl), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18478
Clone ID Related Accession (Same CDS sequence)	NM_168577.3 , NM_001014583.2
Accession Version	NM_001014583.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	breathless, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001014583.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAAAAG TGCCGATCAC GCTGGTGATG ATCATTGCGA TCGTCTCTGC CGCTGCGGAC 
CTGGGATGCG ATTATGGCCA TCATCGTTGC TACATCGATG TGACTGTGGA AAACAGTCCC 
CGCCAGCGCC ATCTGCTCTC CGACATGGAC ATCACGCTGC AGTGCGTCCG ACCCATGGCC 
AAATGGTTCT ATGAGGATAA GTTTCAACTG AGGGCTACCC TTTTGCGACT TGAACGAGCA 
CAGTCCGGAA ATTCTGGAAA CTACGGCTGC CTGGATAGTC AGAACAGATG GTACAATATT 
TCCCTTGTTG TTGGCCATAA GGAACCAGTT GGCAATGATA TCGCCAGCTT TGTCAAGTTG 
GAAGATGCAC CGGCCCTCCC GGAGTCAGAT CTCTTTTTTC AGCCCCTGAA TGAGAGTCGA 
TCCCTGAAGT TGCTGCAACC CCTACCCAAA ACCGTACAAC GGACCGCAGG TGGCCTTTTT 
CAGTTGAACT GCAGTCCCAT GGATCCGGAT GCAAAGGGTG TTAATATATC CTGGCTGCAC 
AATGATACTC AAATCCTTGG TGGACGTGGA CGCATTAAGC TTAAAAGGTG GTCATTAACA 
GTGGGGCAAC TGCAGCCGGA GGATGCTGGA AGCTATCACT GTGAGCTGTG CGTGGAACAG 
GACTGCCAAA GGAGTAATCC GACCCAATTG GAGGTGATAA GCAGAAAGCA CACGGTGCCC 
ATGCTAAAGC CCGGATATCC CCGCAATACC AGCATTGCCC TCGGGGACAA TGTGAGCATT 
GAATGCCTAC TTGAGGACTC TGCCCTGGAG CCGAAGATCA CGTGGCTACA TAAGGGGAAT 
GCGGACAACA TCGATGACCT CTTGCAGCGT TTAAGAGAGC AGTCCCAGCT ACCTGTGGAT 
GTTACCCGAT TGATTACCCG AATGGATGAA CCGCAGGTAC TTCGTCTTGG AAATGTCCTG 
ATGGAGGATG GCGGATGGTA CATATGCATA GCCGAGAACC AGGTTGGACG CACTGTGGCC 
GCCTCCTATG TGGATCTTTA CAGTCCCTCA GATACTACCA CAGTTCGCAC GACCACTACT 
ACCACCGTGG CCAGCCCAAT ACCCACTGCA TCCACCGGAG AGGACAACGA TGACGACGTG 
GAAAATCCAG CAGCGGAAGC ATCAGGCGGC GTTGGTCCAC CTGTGTTCAG AAAAGAGCTG 
AAACGATTAC AGCACTCATT ATCGGGAAAT ACTGTCAATC TAGCCTGTCC AGTGTACGGC 
AAAGCGAACA TCACCTGGAC GAAGGACAAG AAGCCGCTAA ACCGTGAACT CGGCGTCTAT 
GTGCAAAAGA ACTGGACACT CCGCTTCGTG GAGGCCACCA GCGAGGATTC TGGACTATAC 
AACTGTAAGG TGTGCAATGC GTGGGGCTGC ATCCAGTTTG ACTTCAGTGT CCAAATAAAC 
GATCGCACGA GATCCGCACC AATAATCGTG GTGCCGCAAA ATCAGACGGT GAAAGTCAAT 
GGCAGCTTGG TGATGAAGTG CACCGTCTAC TCTGATCTGC ATCCAACAGT CAGCTGGAAA 
AGGGTTGTCC TCAAGAATGC CTCCTTGGAC GGCCTTAAGT CTGTGGAAAT CCAGAATCTC 
AACTTCACCG TGACGAATGA CTCCGTAGTT TTAACGCTTC GTAATGTGAC CTTTGACCAG 
GAGGGCTGGT ACACCTGTTT GGCCTCAAGT GGTCTGGGAA GGAGTAACTC CAGTGTGTAC 
CTTAGGGTAG TGAGTCCTCT GCCACCTTTG GAGATCTACG CCCTGCTGCA CGCCCATCCT 
CTCGGATTCA CCTTGGCCGC TATTACGATA GTGGCTTTGT TCCTTTTGGG CAGTGCTTTC 
ATAACGTTCA TGCTGCGTCG GCTGAGGAGG GAGAAGCTCC TGAAGCTCCG CATCGAAACG 
GTTCATCAGT GGACTAAAAA GGTGATCATA TACCGACCGG GTGGAGAAGA AGGAAGTGGC 
TGCAGCTCCG GCGATCTTCA GATGCCAGTG ATAAGGATCG AGAAGCAGAG AACAACCGTT 
TCCACAACGG GAACTGGCGG TACTGATCCC GCCCAGGGCT TTAATGAGTA TGAGTTCCCG 
CTGGACTCAA ACTGGGAGAT TCCCAGGCAG CAGCTTAGCT TGGGCTCCAT TTTGGGTGAG 
GGCGCCTTTG GACGCGTAGT GATGGCGGAG GCGGAGGGGC TTCCCAGGAG TCCACAACTG 
GCCGAAACCA TCGTGGCCGT CAAGATGGTC AAGGAGGAGC ACACGGATAC GGACATGGCC 
AGTTTGGTGC GTGAAATGGA GGTGATGAAA ATGATTGGCA AGCACATAAA CATCATCAAT 
CTGCTAGGCT GCTGCAGCCA GGGCGGTCCT CTGTGGGTGA TCGTGGAGTA TGCTCCACAC 
GGAAACCTCA AGGACTTCCT CAAGCAGAAT CGACCTGGTG CTCCACAGCG ACGGAGTGAC 
AGTGATGGCT ATCTAGATGA CAAGCCACTG ATTTCCACTC AACATCTTGG CGAAAAGGAG 
CTCACCAAGT TTGCATTTCA AATCGCTCGA GGAATGGAGT ACCTTGCTTC AAGACGGTGC 
ATTCATCGCG ATCTGGCTGC CCGTAATGTC CTTGTAAGCG ATGGATACGT TATGAAGATC 
GCCGATTTTG GTCTGGCGAG GGATATCCAA GATACGGAGT ACTACCGAAA AAATACCAAT 
GGACGGTTGC CCATTAAGTG GATGGCGCCC GAGTCGCTGC AGGAGAAGAA GTACGACTCA 
CAGAGCGACG TCTGGAGCTA TGGTGTTCTG CTGTGGGAGA TCATGACCTA CGGGGATCAA 
CCATACCCGC ACATACTGTC TGCCGAGGAG CTCTACAGCT ACCTGATTAC GGGTCAGCGC 
ATGGAGAAAC CGGCCAAGTG CTCCCTGAAC ATCTACGTGG TAATGCGACA GTGCTGGCAC 
TTTGAGTCCT GTGCACGACC CACGTTCGCG GAGCTGGTAG AGAGTTTCGA TGGGATCCTG 
CAGCAGGCTA GTAGCAATCC AAACGATGCC TATCTGGACC TTTCGATGCC GATGCTGGAG 
ACGCCGCCCT CATCGGGGGA TGAGGATGAT GGCTCCGACA CCGAAACATT CCGGGAAACG 
TCCCCGCTTA GATATCAGTA CACCTATAAG TTTAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014583.1
CDS	221..3379
Translation	MAKVPITLVMIIAIVSAAADLGCDYGHHRCYIDVTVENSPRQRHLLSDMDITLQCVRPMAKWFYEDKFQLRATLLRLERAQSGNSGNYGCLDSQNRWYNISLVVGHKEPVGNDIASFVKLEDAPALPESDLFFQPLNESRSLKLLQPLPKTVQRTAGGLFQLNCSPMDPDAKGVNISWLHNDTQILGGRGRIKLKRWSLTVGQLQPEDAGSYHCELCVEQDCQRSNPTQLEVISRKHTVPMLKPGYPRNTSIALGDNVSIECLLEDSALEPKITWLHKGNADNIDDLLQRLREQSQLPVDVTRLITRMDEPQVLRLGNVLMEDGGWYICIAENQVGRTVAASYVDLYSPSDTTTVRTTTTTTVASPIPTASTGEDNDDDVENPAAEASGGVGPPVFRKELKRLQHSLSGNTVNLACPVYGKANITWTKDKKPLNRELGVYVQKNWTLRFVEATSEDSGLYNCKVCNAWGCIQFDFSVQINDRTRSAPIIVVPQNQTVKVNGSLVMKCTVYSDLHPTVSWKRVVLKNASLDGLKSVEIQNLNFTVTNDSVVLTLRNVTFDQEGWYTCLASSGLGRSNSSVYLRVVSPLPPLEIYALLHAHPLGFTLAAITIVALFLLGSAFITFMLRRLRREKLLKLRIETVHQWTKKVIIYRPGGEEGSGCSSGDLQMPVIRIEKQRTTVSTTGTGGTDPAQGFNEYEFPLDSNWEIPRQQLSLGSILGEGAFGRVVMAEAEGLPRSPQLAETIVAVKMVKEEHTDTDMASLVREMEVMKMIGKHINIINLLGCCSQGGPLWVIVEYAPHGNLKDFLKQNRPGAPQRRSDSDGYLDDKPLISTQHLGEKELTKFAFQIARGMEYLASRRCIHRDLAARNVLVSDGYVMKIADFGLARDIQDTEYYRKNTNGRLPIKWMAPESLQEKKYDSQSDVWSYGVLLWEIMTYGDQPYPHILSAEELYSYLITGQRMEKPAKCSLNIYVVMRQCWHFESCARPTFAELVESFDGILQQASSNPNDAYLDLSMPMLETPPSSGDEDDGSDTETFRETSPLRYQYTYKFN

Target ORF information:

RefSeq Version	NM_001014583.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster breathless (btl), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014583.2

ORF Insert Sequence:

ATGGCAAAAG TGCCGATCAC GCTGGTGATG ATCATTGCGA TCGTCTCTGC CGCTGCGGAC 
CTGGGATGCG ATTATGGCCA TCATCGTTGC TACATCGATG TGACTGTGGA AAACAGTCCC 
CGCCAGCGCC ATCTGCTCTC CGACATGGAC ATCACGCTGC AGTGCGTCCG ACCCATGGCC 
AAATGGTTCT ATGAGGATAA GTTTCAACTG AGGGCTACCC TTTTGCGACT TGAACGAGCA 
CAGTCCGGAA ATTCTGGAAA CTACGGCTGC CTGGATAGTC AGAACAGATG GTACAATATT 
TCCCTTGTTG TTGGCCATAA GGAACCAGTT GGCAATGATA TCGCCAGCTT TGTCAAGTTG 
GAAGATGCAC CGGCCCTCCC GGAGTCAGAT CTCTTTTTTC AGCCCCTGAA TGAGAGTCGA 
TCCCTGAAGT TGCTGCAACC CCTACCCAAA ACCGTACAAC GGACCGCAGG TGGCCTTTTT 
CAGTTGAACT GCAGTCCCAT GGATCCGGAT GCAAAGGGTG TTAATATATC CTGGCTGCAC 
AATGATACTC AAATCCTTGG TGGACGTGGA CGCATTAAGC TTAAAAGGTG GTCATTAACA 
GTGGGGCAAC TGCAGCCGGA GGATGCTGGA AGCTATCACT GTGAGCTGTG CGTGGAACAG 
GACTGCCAAA GGAGTAATCC GACCCAATTG GAGGTGATAA GCAGAAAGCA CACGGTGCCC 
ATGCTAAAGC CCGGATATCC CCGCAATACC AGCATTGCCC TCGGGGACAA TGTGAGCATT 
GAATGCCTAC TTGAGGACTC TGCCCTGGAG CCGAAGATCA CGTGGCTACA TAAGGGGAAT 
GCGGACAACA TCGATGACCT CTTGCAGCGT TTAAGAGAGC AGTCCCAGCT ACCTGTGGAT 
GTTACCCGAT TGATTACCCG AATGGATGAA CCGCAGGTAC TTCGTCTTGG AAATGTCCTG 
ATGGAGGATG GCGGATGGTA CATATGCATA GCCGAGAACC AGGTTGGACG CACTGTGGCC 
GCCTCCTATG TGGATCTTTA CAGTCCCTCA GATACTACCA CAGTTCGCAC GACCACTACT 
ACCACCGTGG CCAGCCCAAT ACCCACTGCA TCCACCGGAG AGGACAACGA TGACGACGTG 
GAAAATCCAG CAGCGGAAGC ATCAGGCGGC GTTGGTCCAC CTGTGTTCAG AAAAGAGCTG 
AAACGATTAC AGCACTCATT ATCGGGAAAT ACTGTCAATC TAGCCTGTCC AGTGTACGGC 
AAAGCGAACA TCACCTGGAC GAAGGACAAG AAGCCGCTAA ACCGTGAACT CGGCGTCTAT 
GTGCAAAAGA ACTGGACACT CCGCTTCGTG GAGGCCACCA GCGAGGATTC TGGACTATAC 
AACTGTAAGG TGTGCAATGC GTGGGGCTGC ATCCAGTTTG ACTTCAGTGT CCAAATAAAC 
GATCGCACGA GATCCGCACC AATAATCGTG GTGCCGCAAA ATCAGACGGT GAAAGTCAAT 
GGCAGCTTGG TGATGAAGTG CACCGTCTAC TCTGATCTGC ATCCAACAGT CAGCTGGAAA 
AGGGTTGTCC TCAAGAATGC CTCCTTGGAC GGCCTTAAGT CTGTGGAAAT CCAGAATCTC 
AACTTCACCG TGACGAATGA CTCCGTAGTT TTAACGCTTC GTAATGTGAC CTTTGACCAG 
GAGGGCTGGT ACACCTGTTT GGCCTCAAGT GGTCTGGGAA GGAGTAACTC CAGTGTGTAC 
CTTAGGGTAG TGAGTCCTCT GCCACCTTTG GAGATCTACG CCCTGCTGCA CGCCCATCCT 
CTCGGATTCA CCTTGGCCGC TATTACGATA GTGGCTTTGT TCCTTTTGGG CAGTGCTTTC 
ATAACGTTCA TGCTGCGTCG GCTGAGGAGG GAGAAGCTCC TGAAGCTCCG CATCGAAACG 
GTTCATCAGT GGACTAAAAA GGTGATCATA TACCGACCGG GTGGAGAAGA AGGAAGTGGC 
TGCAGCTCCG GCGATCTTCA GATGCCAGTG ATAAGGATCG AGAAGCAGAG AACAACCGTT 
TCCACAACGG GAACTGGCGG TACTGATCCC GCCCAGGGCT TTAATGAGTA TGAGTTCCCG 
CTGGACTCAA ACTGGGAGAT TCCCAGGCAG CAGCTTAGCT TGGGCTCCAT TTTGGGTGAG 
GGCGCCTTTG GACGCGTAGT GATGGCGGAG GCGGAGGGGC TTCCCAGGAG TCCACAACTG 
GCCGAAACCA TCGTGGCCGT CAAGATGGTC AAGGAGGAGC ACACGGATAC GGACATGGCC 
AGTTTGGTGC GTGAAATGGA GGTGATGAAA ATGATTGGCA AGCACATAAA CATCATCAAT 
CTGCTAGGCT GCTGCAGCCA GGGCGGTCCT CTGTGGGTGA TCGTGGAGTA TGCTCCACAC 
GGAAACCTCA AGGACTTCCT CAAGCAGAAT CGACCTGGTG CTCCACAGCG ACGGAGTGAC 
AGTGATGGCT ATCTAGATGA CAAGCCACTG ATTTCCACTC AACATCTTGG CGAAAAGGAG 
CTCACCAAGT TTGCATTTCA AATCGCTCGA GGAATGGAGT ACCTTGCTTC AAGACGGTGC 
ATTCATCGCG ATCTGGCTGC CCGTAATGTC CTTGTAAGCG ATGGATACGT TATGAAGATC 
GCCGATTTTG GTCTGGCGAG GGATATCCAA GATACGGAGT ACTACCGAAA AAATACCAAT 
GGACGGTTGC CCATTAAGTG GATGGCGCCC GAGTCGCTGC AGGAGAAGAA GTACGACTCA 
CAGAGCGACG TCTGGAGCTA TGGTGTTCTG CTGTGGGAGA TCATGACCTA CGGGGATCAA 
CCATACCCGC ACATACTGTC TGCCGAGGAG CTCTACAGCT ACCTGATTAC GGGTCAGCGC 
ATGGAGAAAC CGGCCAAGTG CTCCCTGAAC ATCTACGTGG TAATGCGACA GTGCTGGCAC 
TTTGAGTCCT GTGCACGACC CACGTTCGCG GAGCTGGTAG AGAGTTTCGA TGGGATCCTG 
CAGCAGGCTA GTAGCAATCC AAACGATGCC TATCTGGACC TTTCGATGCC GATGCTGGAG 
ACGCCGCCCT CATCGGGGGA TGAGGATGAT GGCTCCGACA CCGAAACATT CCGGGAAACG 
TCCCCGCTTA GATATCAGTA CACCTATAAG TTTAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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btl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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