Frl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Frl
Entrez Gene ID	39561
Full Name	Formin-like
Synonyms	CG13485,CG32138,CG43986,CG6807,Dmel\CG32138,Dmel_CG43986,Q9VUC5_DROME,Q9VUC6_DROME,anon-WO0172774.98,frl
General protein information	Preferred Names Formin-like Names CG32138 gene product from transcript CG32138-RC CG32138-PA CG32138-PB CG32138-PC CG32138-PD Frl-PA Frl-PB Frl-PC Frl-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 70D1-70D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001144476.3	NP_001137948.1	Formin-like, isoform C
NM_001274895.2	NP_001261824.1	Formin-like, isoform D
NM_168575.4	NP_729954.2	Formin-like, isoform A
NM_168576.4	NP_729955.2	Formin-like, isoform B

Pathways from BioSystems

Homologies

Bos taurus (cattle)	FMNL2	NP_001193750.1
Gallus gallus (chicken)	FMNL2	XP_001234981.3
Drosophila melanogaster (fruit fly)	CG32138	NP_729954.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004805	XP_554547.3
	FMNL2	XP_001084256.2
Mus musculus (house mouse)	Fmnl2	NP_765997.2
Danio rerio (zebrafish)	fmnl2a	XP_005168004.1
Homo sapiens (human)	FMNL2	NP_443137.2
Pan troglodytes (chimpanzee)	FMNL2	XP_515836.2
Rattus norvegicus (Norway rat)	RGD1560248	XP_006234258.1
Xenopus tropicalis (tropical clawed frog)	fmnl2	XP_004917741.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Frl likely contributes to ommatidial rotation during eye development; Frl cooperates with DAAM during axon growth in the mushroom body
Title: Unique and Overlapping Functions of Formins Frl and DAAM During Ommatidial Rotation and Neuronal Development in Drosophila.

The following Frl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Frl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18475	NM_001144476.3 Latest version!	Drosophila melanogaster Formin-like (Frl), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa18476	NM_168575.4 Latest version!	Drosophila melanogaster Formin-like (Frl), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa18477	NM_168576.4 Latest version!	Drosophila melanogaster Formin-like (Frl), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa20936	NM_001274895.2 Latest version!	Drosophila melanogaster Formin-like (Frl), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Frl ( NM_001144476.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137948.1 Drosophila melanogaster Formin-like (Frl), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa18475
Clone ID Related Accession (Same CDS sequence)	NM_001144476.3
Accession Version	NM_001144476.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Formin-like, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Oct 19, 2015 this sequence version replaced NM_001144476.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGCCG TCAAATCGCG AACCATCACC AGCGCTGATG TGGACGCCGA TGAGCAGCTG 
CAGCATCCCC ATTCGCATGC GCACCATCAC TCGATGCGCA ATGGCCACCA GCACAACGGT 
TCCATCTCTA GCGGCACGCT GCAGAAACAG GATCTGCGCT ACGACATCGG CTGCAGTTCC 
CAGTACCAAC ATGTCAGGCA GCCGAGCCTG CGGAGCCGGA GCCAGCAACC CATGCCCACC 
ACAGATGAGC TGGACCGACG ATTCGCCAAA GTGCTGGCGT CGATGGACTT GCCACCGGAT 
AAAGCAAAGT TGCTGCGGAA CTATGATGAC GAGAAGAAAT GGGACATGAT ATGCGATCAA 
GAAATGGTGC AGGCAAAGGA TCCGCCCTCA CATTACTTGA GTAAACTGCG CACATATCTG 
GACCCAAAGG CATCAAGGAG TCATCGGAAA CGGAAAATGG TCGGCGAGTC CACGTCCACC 
CAGGTGCTCC GCGATCTGGA GATCTCGCTG CGCACGAACC ACATCGAGTG GGTGAAGGAG 
TTCCTGGATG ACACGAACCA GGGTCTGGAC GCCCTGGTCG ACTATCTCAG CTTCCGACTG 
CAGATGATGC GACACGAGCA GCGCCTTCAG GGTGTCTTGT GTGCCTCGGA GGAGCGTCTG 
AATCTCACAA ACGGCGGCGA TGGCGGTGAG ATAGTGATGG GAAACAGTAG TTCTGTTAGT 
CCTGGTGGAG GTGGTGGTTT ACTATCACAT GGAAACAGTA CGGGACATGG TCTGGCCAAT 
GGCACACTTG ACTCGAGGCA GCAGCACACA ATGTCCTATG GATTCCTACG ACCTACCATT 
GCCGATGCTC TGGATAGTCC TAGTTTGAAG CGAAGGTCAC GACATATTGC CAAATTAAAC 
ATGGGTGCCG CCACGGACGA CATCCATGTG TCCATTATGT GCCTGCGAGC TATCATGAAC 
AATAAGTATG GGTTCAACAT GGTTATCCAG CATCGCGAGG CCATCAACTG CATTGCCTTG 
AGTCTTATCC ACAAATCGCT GAGGACGAAA GCCCTGGTCC TGGAGCTGCT GGCAGCCATC 
TGTCTGGTAA AGGGAGGACA CGAAATCATT TTGGGTTCGT TCGATAATTT TAAGGATGTG 
TGCCAGGAGA AGCGACGCTT CCAAACGCTC ATGGAGTACT TTATGAACTT CGAGGCCTTT 
AACATAGATT TTATGGTTGC CTGCATGCAG TTCATGAACA TCGTTGTCCA CTCGGTGGAG 
GACATGAACT ACAGGGTGCA CTTACAGTAC GAGTTTACAG CCCTGGGCTT GGATAAGTAT 
CTGGAGCGAA TTCGATTGAC AGAATCGGAG GAACTGAAGG TGCAGATATC AGCCTATTTG 
GACAACGTCT TTGATGTTGC TGCCTTGATG GAGGATTCCG AGACAAAAAC TTCAGCCCTG 
GAACGAGTCC AAGAGCTTGA GGATCAACTT GAGCGAGAAA TAGATCGTAA CTCAGAGTTC 
CTCTATAAGT ATGCGGAATT AGAGTCCGAG AGTCTAACGC TGAAAACGGA ACGCGAGCAG 
CTGGCTATGA TTCGGCAGAA GCTGGAGGAG GAACTTACAG TGATGCAGCG AATGTTGCAG 
CACAACGAGC AGGAGCTGAA GAAACGGGAC ACACTGCTGC ACACAAAGAA CATGGAGCTG 
CAGACGCTTT CGCGTTCCCT GCCACGATCC GCCTCCAGCG GCGATGGTTC TCTGGCGAAT 
GGTGGCCTCA TGGCTGGTTC CACATCGGGG GCAGCCTCTC TAACATTGCC ACCACCTCCG 
CCGCCAATGC CCGCCTCGCC TACTGCAAGT TCAGCTGCTC CTCCACCACC TCCGCCGCCA 
GCACCACCGG CTCCACCACC ACCGCCGGGC TTCAGTCCGC TGGGCAGTCC GAGCGGCAGC 
CTAGCCTCGA CAGCGCCATC GCCGCCACAT GCCCCGCCCA TGCTAAGCTC CTTCCAACCG 
CCACCGCCTC CAGTGGCCGG CTTTATGCCC GCTCCCGATG GCGCCATGAC CATCAAACGC 
AAGGTGCCCA CTAAATACAA GTTGCCCACC TTGAACTGGA TAGCACTAAA GCCTAATCAG 
GTACGTGGTA CAATATTCAA CGAGCTGGAT GACGAAAAGA TCTTCAAGCA AATCGACTTC 
AATGAGTTTG AGGAGCGCTT CAAGATCGGG ATTGGCGGTG CTTTGCGCAA TGGTAGCAAT 
GGAACCGAGG TCGATGGGTC GCTGCAGTCC AGCAAACGCT TCAAGAGGCC CGACAATGTC 
TCGCTGCTGG AGCACACGAG GTTAAGAAAC ATTGCAATCT CCCGTCGCAA GCTGGGTATG 
CCCATTGATG ATGTCATCGC CGCCATTCAT AGTCTGGACC TGAAGAAACT TTCCCTGGAG 
AACGTCGAGC TGCTGCAAAA AATGGTGCCC ACGGATGCCG AGGTCAAATC CTACAAGGAA 
TATATCATCG AGCGCAAGGA CCAACAGCTA CTCACCGAAG AAGACAAGTT TATGCTGCAG 
TTGTCGCGTG TGGAGCGTAT CTCGTCCAAG CTAGCCATTA TGAACTATAT GGGCAATTTT 
GTCGACAGCG TTCATCTCAT TAGTCCGCAA GTGCAATCGA TAGCAGGAGC GTCGACTTCC 
TTAAAACAAT CTCGAAAATT CAAGGCGGTT TTGGAAATTG TCCTGGCTTT CGGCAACTAT 
CTCAACAGCA ACAAACGGGG ACCAGCCTAT GGCTTTAAGC TGCAATCGCT GGACACGCTG 
ATCGATACAA AATCCACAGA CAAGCGATCG TCACTGCTTC ACTATATTGT GGCCACCATA 
CGGGCCAAAT TTCCGGAGCT GCTGAACTTC GAGAGCGAGC TGTATGGAAC AGACAAGGCT 
GCATCGGTGG CACTAGAGAA TGTGGTGGCC GATGTTCAGG AGCTTGAAAA GGGCATGGAT 
CTGGTGCGCA AGGAGGCCGA GCTGCGAGTG AAGGGTGCCC AGACGCATAT CCTGCGTGAC 
TTCCTGAACA ACAGCGAGGA CAAGCTGAAG AAGATCAAGA GCGATCTGCG GCATGCACAG 
GAAGCGTTCA AGGAGTGCGT TGAGTACTTT GGCGACTCCT CGCGGAATGC AGATGCGGCT 
GCTTTCTTTG CGTTGATCGT ACGCTTCACG AGAGCGTTTA AGCAACACGA TCAGGAGAAC 
GAGCAGCGTC TTCGCCTGGA AAAGGCCGCT GCGCTGGCCG CTTCCAAGAA AGAGAACGAT 
CAGGTGCTTA TGCGCAACAA GGTTAACCAG AAGAAGCAAC AGTTGCCCTC AAACGGCGCA 
TTATCCTTGC AGGAAGCTGT CATAAACGAG CTGAAGAGCA AGGCGCACTC GGTGCGCGAG 
AAAAAGCTGC TGCAGCAGGA CGAGGTGTAC AACGGAGCCC TGGAGGACAT CCTGCTCGGC 
CTGAAGAGCG AGCCGTACAG GCGGGCGGAT GCTGTGCGGC GGTCGCAGCG CCGGAGGATC 
GACAATAATC GTTTATCGCG CACCCTGGAG GAAATGGATT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137948.1
CDS	854..4378
Translation	MGAVKSRTITSADVDADEQLQHPHSHAHHHSMRNGHQHNGSISSGTLQKQDLRYDIGCSSQYQHVRQPSLRSRSQQPMPTTDELDRRFAKVLASMDLPPDKAKLLRNYDDEKKWDMICDQEMVQAKDPPSHYLSKLRTYLDPKASRSHRKRKMVGESTSTQVLRDLEISLRTNHIEWVKEFLDDTNQGLDALVDYLSFRLQMMRHEQRLQGVLCASEERLNLTNGGDGGEIVMGNSSSVSPGGGGGLLSHGNSTGHGLANGTLDSRQQHTMSYGFLRPTIADALDSPSLKRRSRHIAKLNMGAATDDIHVSIMCLRAIMNNKYGFNMVIQHREAINCIALSLIHKSLRTKALVLELLAAICLVKGGHEIILGSFDNFKDVCQEKRRFQTLMEYFMNFEAFNIDFMVACMQFMNIVVHSVEDMNYRVHLQYEFTALGLDKYLERIRLTESEELKVQISAYLDNVFDVAALMEDSETKTSALERVQELEDQLEREIDRNSEFLYKYAELESESLTLKTEREQLAMIRQKLEEELTVMQRMLQHNEQELKKRDTLLHTKNMELQTLSRSLPRSASSGDGSLANGGLMAGSTSGAASLTLPPPPPPMPASPTASSAAPPPPPPPAPPAPPPPPGFSPLGSPSGSLASTAPSPPHAPPMLSSFQPPPPPVAGFMPAPDGAMTIKRKVPTKYKLPTLNWIALKPNQVRGTIFNELDDEKIFKQIDFNEFEERFKIGIGGALRNGSNGTEVDGSLQSSKRFKRPDNVSLLEHTRLRNIAISRRKLGMPIDDVIAAIHSLDLKKLSLENVELLQKMVPTDAEVKSYKEYIIERKDQQLLTEEDKFMLQLSRVERISSKLAIMNYMGNFVDSVHLISPQVQSIAGASTSLKQSRKFKAVLEIVLAFGNYLNSNKRGPAYGFKLQSLDTLIDTKSTDKRSSLLHYIVATIRAKFPELLNFESELYGTDKAASVALENVVADVQELEKGMDLVRKEAELRVKGAQTHILRDFLNNSEDKLKKIKSDLRHAQEAFKECVEYFGDSSRNADAAAFFALIVRFTRAFKQHDQENEQRLRLEKAAALAASKKENDQVLMRNKVNQKKQQLPSNGALSLQEAVINELKSKAHSVREKKLLQQDEVYNGALEDILLGLKSEPYRRADAVRRSQRRRIDNNRLSRTLEEMDC

Target ORF information:

RefSeq Version	NM_001144476.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Formin-like (Frl), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144476.3

ORF Insert Sequence:

ATGGGCGCCG TCAAATCGCG AACCATCACC AGCGCTGATG TGGACGCCGA TGAGCAGCTG 
CAGCATCCCC ATTCGCATGC GCACCATCAC TCGATGCGCA ATGGCCACCA GCACAACGGT 
TCCATCTCTA GCGGCACGCT GCAGAAACAG GATCTGCGCT ACGACATCGG CTGCAGTTCC 
CAGTACCAAC ATGTCAGGCA GCCGAGCCTG CGGAGCCGGA GCCAGCAACC CATGCCCACC 
ACAGATGAGC TGGACCGACG ATTCGCCAAA GTGCTGGCGT CGATGGACTT GCCACCGGAT 
AAAGCAAAGT TGCTGCGGAA CTATGATGAC GAGAAGAAAT GGGACATGAT ATGCGATCAA 
GAAATGGTGC AGGCAAAGGA TCCGCCCTCA CATTACTTGA GTAAACTGCG CACATATCTG 
GACCCAAAGG CATCAAGGAG TCATCGGAAA CGGAAAATGG TCGGCGAGTC CACGTCCACC 
CAGGTGCTCC GCGATCTGGA GATCTCGCTG CGCACGAACC ACATCGAGTG GGTGAAGGAG 
TTCCTGGATG ACACGAACCA GGGTCTGGAC GCCCTGGTCG ACTATCTCAG CTTCCGACTG 
CAGATGATGC GACACGAGCA GCGCCTTCAG GGTGTCTTGT GTGCCTCGGA GGAGCGTCTG 
AATCTCACAA ACGGCGGCGA TGGCGGTGAG ATAGTGATGG GAAACAGTAG TTCTGTTAGT 
CCTGGTGGAG GTGGTGGTTT ACTATCACAT GGAAACAGTA CGGGACATGG TCTGGCCAAT 
GGCACACTTG ACTCGAGGCA GCAGCACACA ATGTCCTATG GATTCCTACG ACCTACCATT 
GCCGATGCTC TGGATAGTCC TAGTTTGAAG CGAAGGTCAC GACATATTGC CAAATTAAAC 
ATGGGTGCCG CCACGGACGA CATCCATGTG TCCATTATGT GCCTGCGAGC TATCATGAAC 
AATAAGTATG GGTTCAACAT GGTTATCCAG CATCGCGAGG CCATCAACTG CATTGCCTTG 
AGTCTTATCC ACAAATCGCT GAGGACGAAA GCCCTGGTCC TGGAGCTGCT GGCAGCCATC 
TGTCTGGTAA AGGGAGGACA CGAAATCATT TTGGGTTCGT TCGATAATTT TAAGGATGTG 
TGCCAGGAGA AGCGACGCTT CCAAACGCTC ATGGAGTACT TTATGAACTT CGAGGCCTTT 
AACATAGATT TTATGGTTGC CTGCATGCAG TTCATGAACA TCGTTGTCCA CTCGGTGGAG 
GACATGAACT ACAGGGTGCA CTTACAGTAC GAGTTTACAG CCCTGGGCTT GGATAAGTAT 
CTGGAGCGAA TTCGATTGAC AGAATCGGAG GAACTGAAGG TGCAGATATC AGCCTATTTG 
GACAACGTCT TTGATGTTGC TGCCTTGATG GAGGATTCCG AGACAAAAAC TTCAGCCCTG 
GAACGAGTCC AAGAGCTTGA GGATCAACTT GAGCGAGAAA TAGATCGTAA CTCAGAGTTC 
CTCTATAAGT ATGCGGAATT AGAGTCCGAG AGTCTAACGC TGAAAACGGA ACGCGAGCAG 
CTGGCTATGA TTCGGCAGAA GCTGGAGGAG GAACTTACAG TGATGCAGCG AATGTTGCAG 
CACAACGAGC AGGAGCTGAA GAAACGGGAC ACACTGCTGC ACACAAAGAA CATGGAGCTG 
CAGACGCTTT CGCGTTCCCT GCCACGATCC GCCTCCAGCG GCGATGGTTC TCTGGCGAAT 
GGTGGCCTCA TGGCTGGTTC CACATCGGGG GCAGCCTCTC TAACATTGCC ACCACCTCCG 
CCGCCAATGC CCGCCTCGCC TACTGCAAGT TCAGCTGCTC CTCCACCACC TCCGCCGCCA 
GCACCACCGG CTCCACCACC ACCGCCGGGC TTCAGTCCGC TGGGCAGTCC GAGCGGCAGC 
CTAGCCTCGA CAGCGCCATC GCCGCCACAT GCCCCGCCCA TGCTAAGCTC CTTCCAACCG 
CCACCGCCTC CAGTGGCCGG CTTTATGCCC GCTCCCGATG GCGCCATGAC CATCAAACGC 
AAGGTGCCCA CTAAATACAA GTTGCCCACC TTGAACTGGA TAGCACTAAA GCCTAATCAG 
GTACGTGGTA CAATATTCAA CGAGCTGGAT GACGAAAAGA TCTTCAAGCA AATCGACTTC 
AATGAGTTTG AGGAGCGCTT CAAGATCGGG ATTGGCGGTG CTTTGCGCAA TGGTAGCAAT 
GGAACCGAGG TCGATGGGTC GCTGCAGTCC AGCAAACGCT TCAAGAGGCC CGACAATGTC 
TCGCTGCTGG AGCACACGAG GTTAAGAAAC ATTGCAATCT CCCGTCGCAA GCTGGGTATG 
CCCATTGATG ATGTCATCGC CGCCATTCAT AGTCTGGACC TGAAGAAACT TTCCCTGGAG 
AACGTCGAGC TGCTGCAAAA AATGGTGCCC ACGGATGCCG AGGTCAAATC CTACAAGGAA 
TATATCATCG AGCGCAAGGA CCAACAGCTA CTCACCGAAG AAGACAAGTT TATGCTGCAG 
TTGTCGCGTG TGGAGCGTAT CTCGTCCAAG CTAGCCATTA TGAACTATAT GGGCAATTTT 
GTCGACAGCG TTCATCTCAT TAGTCCGCAA GTGCAATCGA TAGCAGGAGC GTCGACTTCC 
TTAAAACAAT CTCGAAAATT CAAGGCGGTT TTGGAAATTG TCCTGGCTTT CGGCAACTAT 
CTCAACAGCA ACAAACGGGG ACCAGCCTAT GGCTTTAAGC TGCAATCGCT GGACACGCTG 
ATCGATACAA AATCCACAGA CAAGCGATCG TCACTGCTTC ACTATATTGT GGCCACCATA 
CGGGCCAAAT TTCCGGAGCT GCTGAACTTC GAGAGCGAGC TGTATGGAAC AGACAAGGCT 
GCATCGGTGG CACTAGAGAA TGTGGTGGCC GATGTTCAGG AGCTTGAAAA GGGCATGGAT 
CTGGTGCGCA AGGAGGCCGA GCTGCGAGTG AAGGGTGCCC AGACGCATAT CCTGCGTGAC 
TTCCTGAACA ACAGCGAGGA CAAGCTGAAG AAGATCAAGA GCGATCTGCG GCATGCACAG 
GAAGCGTTCA AGGAGTGCGT TGAGTACTTT GGCGACTCCT CGCGGAATGC AGATGCGGCT 
GCTTTCTTTG CGTTGATCGT ACGCTTCACG AGAGCGTTTA AGCAACACGA TCAGGAGAAC 
GAGCAGCGTC TTCGCCTGGA AAAGGCCGCT GCGCTGGCCG CTTCCAAGAA AGAGAACGAT 
CAGGTGCTTA TGCGCAACAA GGTTAACCAG AAGAAGCAAC AGTTGCCCTC AAACGGCGCA 
TTATCCTTGC AGGAAGCTGT CATAAACGAG CTGAAGAGCA AGGCGCACTC GGTGCGCGAG 
AAAAAGCTGC TGCAGCAGGA CGAGGTGTAC AACGGAGCCC TGGAGGACAT CCTGCTCGGC 
CTGAAGAGCG AGCCGTACAG GCGGGCGGAT GCTGTGCGGC GGTCGCAGCG CCGGAGGATC 
GACAATAATC GTTTATCGCG CACCCTGGAG GAAATGGATT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Frl ( NM_168575.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729954.2 Drosophila melanogaster Formin-like (Frl), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18476
Clone ID Related Accession (Same CDS sequence)	NM_168575.4
Accession Version	NM_168575.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Formin-like, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Oct 19, 2015 this sequence version replaced NM_168575.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGCCG TCAAATCGCG AACCATCACC AGCGCTGATG TGGACGCCGA TGAGCAGCTG 
CAGCATCCCC ATTCGCATGC GCACCATCAC TCGATGCGCA ATGGCCACCA GCACAACGGT 
TCCATCTCTA GCGGCACGCT GCAGAAACAG GATCTGCGCT ACGACATCGG CTGCAGTTCC 
CAGTACCAAC ATGTCAGGCA GCCGAGCCTG CGGAGCCGGA GCCAGCAACC CATGCCCACC 
ACAGATGAGC TGGACCGACG ATTCGCCAAA GTGCTGGCGT CGATGGACTT GCCACCGGAT 
AAAGCAAAGT TGCTGCGGAA CTATGATGAC GAGAAGAAAT GGGACATGAT ATGCGATCAA 
GAAATGGTGC AGGCAAAGGA TCCGCCCTCA CATTACTTGA GTAAACTGCG CACATATCTG 
GACCCAAAGG CATCAAGGAG TCATCGGCTT TATCTCTTCT ACTTTCTTTG TCAGAAACGG 
AAAATGGTCG GCGAGTCCAC GTCCACCCAG GTGCTCCGCG ATCTGGAGAT CTCGCTGCGC 
ACGAACCACA TCGAGTGGGT GAAGGAGTTC CTGGATGACA CGAACCAGGG TCTGGACGCC 
CTGGTCGACT ATCTCAGCTT CCGACTGCAG ATGATGCGAC ACGAGCAGCG CCTTCAGGGT 
GTCTTGTGTG CCTCGGAGGA GCGTCTGAAT CTCACAAACG GCGGCGATGG CGGTGAGATA 
GTGATGGGAA ACAGTAGTTC TGTTAGTCCT GGTGGAGGTG GTGGTTTACT ATCACATGGA 
AACAGTACGG GACATGGTCT GGCCAATGGC ACACTTGACT CGAGGCAGCA GCACACAATG 
TCCTATGGAT TCCTACGACC TACCATTGCC GATGCTCTGG ATAGTCCTAG TTTGAAGCGA 
AGGTCACGAC ATATTGCCAA ATTAAACATG GGTGCCGCCA CGGACGACAT CCATGTGTCC 
ATTATGTGCC TGCGAGCTAT CATGAACAAT AAGTATGGGT TCAACATGGT TATCCAGCAT 
CGCGAGGCCA TCAACTGCAT TGCCTTGAGT CTTATCCACA AATCGCTGAG GACGAAAGCC 
CTGGTCCTGG AGCTGCTGGC AGCCATCTGT CTGGTAAAGG GAGGACACGA AATCATTTTG 
GGTTCGTTCG ATAATTTTAA GGATGTGTGC CAGGAGAAGC GACGCTTCCA AACGCTCATG 
GAGTACTTTA TGAACTTCGA GGCCTTTAAC ATAGATTTTA TGGTTGCCTG CATGCAGTTC 
ATGAACATCG TTGTCCACTC GGTGGAGGAC ATGAACTACA GGGTGCACTT ACAGTACGAG 
TTTACAGCCC TGGGCTTGGA TAAGTATCTG GAGCGAATTC GATTGACAGA ATCGGAGGAA 
CTGAAGGTGC AGATATCAGC CTATTTGGAC AACGTCTTTG ATGTTGCTGC CTTGATGGAG 
GATTCCGAGA CAAAAACTTC AGCCCTGGAA CGAGTCCAAG AGCTTGAGGA TCAACTTGAG 
CGAGAAATAG ATCGTAACTC AGAGTTCCTC TATAAGTATG CGGAATTAGA GTCCGAGAGT 
CTAACGCTGA AAACGGAACG CGAGCAGCTG GCTATGATTC GGCAGAAGCT GGAGGAGGAA 
CTTACAGTGA TGCAGCGAAT GTTGCAGCAC AACGAGCAGG AGCTGAAGAA ACGGGACACA 
CTGCTGCACA CAAAGAACAT GGAGCTGCAG ACGCTTTCGC GTTCCCTGCC ACGATCCGCC 
TCCAGCGGCG ATGGTTCTCT GGCGAATGGT GGCCTCATGG CTGGTTCCAC ATCGGGGGCA 
GCCTCTCTAA CATTGCCACC ACCTCCGCCG CCAATGCCCG CCTCGCCTAC TGCAAGTTCA 
GCTGCTCCTC CACCACCTCC GCCGCCAGCA CCACCGGCTC CACCACCACC GCCGGGCTTC 
AGTCCGCTGG GCAGTCCGAG CGGCAGCCTA GCCTCGACAG CGCCATCGCC GCCACATGCC 
CCGCCCATGC TAAGCTCCTT CCAACCGCCA CCGCCTCCAG TGGCCGGCTT TATGCCCGCT 
CCCGATGGCG CCATGACCAT CAAACGCAAG GTGCCCACTA AATACAAGTT GCCCACCTTG 
AACTGGATAG CACTAAAGCC TAATCAGGTA CGTGGTACAA TATTCAACGA GCTGGATGAC 
GAAAAGATCT TCAAGCAAAT CGACTTCAAT GAGTTTGAGG AGCGCTTCAA GATCGGGATT 
GGCGGTGCTT TGCGCAATGG TAGCAATGGA ACCGAGGTCG ATGGGTCGCT GCAGTCCAGC 
AAACGCTTCA AGAGGCCCGA CAATGTCTCG CTGCTGGAGC ACACGAGGTT AAGAAACATT 
GCAATCTCCC GTCGCAAGCT GGGTATGCCC ATTGATGATG TCATCGCCGC CATTCATAGT 
CTGGACCTGA AGAAACTTTC CCTGGAGAAC GTCGAGCTGC TGCAAAAAAT GGTGCCCACG 
GATGCCGAGG TCAAATCCTA CAAGGAATAT ATCATCGAGC GCAAGGACCA ACAGCTACTC 
ACCGAAGAAG ACAAGTTTAT GCTGCAGTTG TCGCGTGTGG AGCGTATCTC GTCCAAGCTA 
GCCATTATGA ACTATATGGG CAATTTTGTC GACAGCGTTC ATCTCATTAG TCCGCAAGTG 
CAATCGATAG CAGGAGCGTC GACTTCCTTA AAACAATCTC GAAAATTCAA GGCGGTTTTG 
GAAATTGTCC TGGCTTTCGG CAACTATCTC AACAGCAACA AACGGGGACC AGCCTATGGC 
TTTAAGCTGC AATCGCTGGA CACGCTGATC GATACAAAAT CCACAGACAA GCGATCGTCA 
CTGCTTCACT ATATTGTGGC CACCATACGG GCCAAATTTC CGGAGCTGCT GAACTTCGAG 
AGCGAGCTGT ATGGAACAGA CAAGGCTGCA TCGGTGGCAC TAGAGAATGT GGTGGCCGAT 
GTTCAGGAGC TTGAAAAGGG CATGGATCTG GTGCGCAAGG AGGCCGAGCT GCGAGTGAAG 
GGTGCCCAGA CGCATATCCT GCGTGACTTC CTGAACAACA GCGAGGACAA GCTGAAGAAG 
ATCAAGAGCG ATCTGCGGCA TGCACAGGAA GCGTTCAAGG AGTGCGTTGA GTACTTTGGC 
GACTCCTCGC GGAATGCAGA TGCGGCTGCT TTCTTTGCGT TGATCGTACG CTTCACGAGA 
GCGTTTAAGC AACACGATCA GGAGAACGAG CAGCGTCTTC GCCTGGAAAA GGCCGCTGCG 
CTGGCCGCTT CCAAGAAAGA GAACGATCAG GTGCTTATGC GCAACAAGGT TAACCAGAAG 
AAGCAACAGT TGCCCTCAAA CGGCGCATTA TCCTTGCAGG AAGCTGTCAT AAACGAGCTG 
AAGAGCAAGG CGCACTCGGT GCGCGAGAAA AAGCTGCTGC AGCAGGACGA GGTGTACAAC 
GGAGCCCTGG AGGACATCCT GCTCGGCCTG AAGAGCGAGC CGTACAGGCG GGCGGATGCT 
GTGCGGCGGT CGCAGCGCCG GAGGATCGAC AATAATCGTT TATCGCGCAC CCTGGAGGAA 
ATGGATTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729954.2
CDS	854..4405
Translation	MGAVKSRTITSADVDADEQLQHPHSHAHHHSMRNGHQHNGSISSGTLQKQDLRYDIGCSSQYQHVRQPSLRSRSQQPMPTTDELDRRFAKVLASMDLPPDKAKLLRNYDDEKKWDMICDQEMVQAKDPPSHYLSKLRTYLDPKASRSHRLYLFYFLCQKRKMVGESTSTQVLRDLEISLRTNHIEWVKEFLDDTNQGLDALVDYLSFRLQMMRHEQRLQGVLCASEERLNLTNGGDGGEIVMGNSSSVSPGGGGGLLSHGNSTGHGLANGTLDSRQQHTMSYGFLRPTIADALDSPSLKRRSRHIAKLNMGAATDDIHVSIMCLRAIMNNKYGFNMVIQHREAINCIALSLIHKSLRTKALVLELLAAICLVKGGHEIILGSFDNFKDVCQEKRRFQTLMEYFMNFEAFNIDFMVACMQFMNIVVHSVEDMNYRVHLQYEFTALGLDKYLERIRLTESEELKVQISAYLDNVFDVAALMEDSETKTSALERVQELEDQLEREIDRNSEFLYKYAELESESLTLKTEREQLAMIRQKLEEELTVMQRMLQHNEQELKKRDTLLHTKNMELQTLSRSLPRSASSGDGSLANGGLMAGSTSGAASLTLPPPPPPMPASPTASSAAPPPPPPPAPPAPPPPPGFSPLGSPSGSLASTAPSPPHAPPMLSSFQPPPPPVAGFMPAPDGAMTIKRKVPTKYKLPTLNWIALKPNQVRGTIFNELDDEKIFKQIDFNEFEERFKIGIGGALRNGSNGTEVDGSLQSSKRFKRPDNVSLLEHTRLRNIAISRRKLGMPIDDVIAAIHSLDLKKLSLENVELLQKMVPTDAEVKSYKEYIIERKDQQLLTEEDKFMLQLSRVERISSKLAIMNYMGNFVDSVHLISPQVQSIAGASTSLKQSRKFKAVLEIVLAFGNYLNSNKRGPAYGFKLQSLDTLIDTKSTDKRSSLLHYIVATIRAKFPELLNFESELYGTDKAASVALENVVADVQELEKGMDLVRKEAELRVKGAQTHILRDFLNNSEDKLKKIKSDLRHAQEAFKECVEYFGDSSRNADAAAFFALIVRFTRAFKQHDQENEQRLRLEKAAALAASKKENDQVLMRNKVNQKKQQLPSNGALSLQEAVINELKSKAHSVREKKLLQQDEVYNGALEDILLGLKSEPYRRADAVRRSQRRRIDNNRLSRTLEEMDC

Target ORF information:

RefSeq Version	NM_168575.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Formin-like (Frl), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168575.4

ORF Insert Sequence:

ATGGGCGCCG TCAAATCGCG AACCATCACC AGCGCTGATG TGGACGCCGA TGAGCAGCTG 
CAGCATCCCC ATTCGCATGC GCACCATCAC TCGATGCGCA ATGGCCACCA GCACAACGGT 
TCCATCTCTA GCGGCACGCT GCAGAAACAG GATCTGCGCT ACGACATCGG CTGCAGTTCC 
CAGTACCAAC ATGTCAGGCA GCCGAGCCTG CGGAGCCGGA GCCAGCAACC CATGCCCACC 
ACAGATGAGC TGGACCGACG ATTCGCCAAA GTGCTGGCGT CGATGGACTT GCCACCGGAT 
AAAGCAAAGT TGCTGCGGAA CTATGATGAC GAGAAGAAAT GGGACATGAT ATGCGATCAA 
GAAATGGTGC AGGCAAAGGA TCCGCCCTCA CATTACTTGA GTAAACTGCG CACATATCTG 
GACCCAAAGG CATCAAGGAG TCATCGGCTT TATCTCTTCT ACTTTCTTTG TCAGAAACGG 
AAAATGGTCG GCGAGTCCAC GTCCACCCAG GTGCTCCGCG ATCTGGAGAT CTCGCTGCGC 
ACGAACCACA TCGAGTGGGT GAAGGAGTTC CTGGATGACA CGAACCAGGG TCTGGACGCC 
CTGGTCGACT ATCTCAGCTT CCGACTGCAG ATGATGCGAC ACGAGCAGCG CCTTCAGGGT 
GTCTTGTGTG CCTCGGAGGA GCGTCTGAAT CTCACAAACG GCGGCGATGG CGGTGAGATA 
GTGATGGGAA ACAGTAGTTC TGTTAGTCCT GGTGGAGGTG GTGGTTTACT ATCACATGGA 
AACAGTACGG GACATGGTCT GGCCAATGGC ACACTTGACT CGAGGCAGCA GCACACAATG 
TCCTATGGAT TCCTACGACC TACCATTGCC GATGCTCTGG ATAGTCCTAG TTTGAAGCGA 
AGGTCACGAC ATATTGCCAA ATTAAACATG GGTGCCGCCA CGGACGACAT CCATGTGTCC 
ATTATGTGCC TGCGAGCTAT CATGAACAAT AAGTATGGGT TCAACATGGT TATCCAGCAT 
CGCGAGGCCA TCAACTGCAT TGCCTTGAGT CTTATCCACA AATCGCTGAG GACGAAAGCC 
CTGGTCCTGG AGCTGCTGGC AGCCATCTGT CTGGTAAAGG GAGGACACGA AATCATTTTG 
GGTTCGTTCG ATAATTTTAA GGATGTGTGC CAGGAGAAGC GACGCTTCCA AACGCTCATG 
GAGTACTTTA TGAACTTCGA GGCCTTTAAC ATAGATTTTA TGGTTGCCTG CATGCAGTTC 
ATGAACATCG TTGTCCACTC GGTGGAGGAC ATGAACTACA GGGTGCACTT ACAGTACGAG 
TTTACAGCCC TGGGCTTGGA TAAGTATCTG GAGCGAATTC GATTGACAGA ATCGGAGGAA 
CTGAAGGTGC AGATATCAGC CTATTTGGAC AACGTCTTTG ATGTTGCTGC CTTGATGGAG 
GATTCCGAGA CAAAAACTTC AGCCCTGGAA CGAGTCCAAG AGCTTGAGGA TCAACTTGAG 
CGAGAAATAG ATCGTAACTC AGAGTTCCTC TATAAGTATG CGGAATTAGA GTCCGAGAGT 
CTAACGCTGA AAACGGAACG CGAGCAGCTG GCTATGATTC GGCAGAAGCT GGAGGAGGAA 
CTTACAGTGA TGCAGCGAAT GTTGCAGCAC AACGAGCAGG AGCTGAAGAA ACGGGACACA 
CTGCTGCACA CAAAGAACAT GGAGCTGCAG ACGCTTTCGC GTTCCCTGCC ACGATCCGCC 
TCCAGCGGCG ATGGTTCTCT GGCGAATGGT GGCCTCATGG CTGGTTCCAC ATCGGGGGCA 
GCCTCTCTAA CATTGCCACC ACCTCCGCCG CCAATGCCCG CCTCGCCTAC TGCAAGTTCA 
GCTGCTCCTC CACCACCTCC GCCGCCAGCA CCACCGGCTC CACCACCACC GCCGGGCTTC 
AGTCCGCTGG GCAGTCCGAG CGGCAGCCTA GCCTCGACAG CGCCATCGCC GCCACATGCC 
CCGCCCATGC TAAGCTCCTT CCAACCGCCA CCGCCTCCAG TGGCCGGCTT TATGCCCGCT 
CCCGATGGCG CCATGACCAT CAAACGCAAG GTGCCCACTA AATACAAGTT GCCCACCTTG 
AACTGGATAG CACTAAAGCC TAATCAGGTA CGTGGTACAA TATTCAACGA GCTGGATGAC 
GAAAAGATCT TCAAGCAAAT CGACTTCAAT GAGTTTGAGG AGCGCTTCAA GATCGGGATT 
GGCGGTGCTT TGCGCAATGG TAGCAATGGA ACCGAGGTCG ATGGGTCGCT GCAGTCCAGC 
AAACGCTTCA AGAGGCCCGA CAATGTCTCG CTGCTGGAGC ACACGAGGTT AAGAAACATT 
GCAATCTCCC GTCGCAAGCT GGGTATGCCC ATTGATGATG TCATCGCCGC CATTCATAGT 
CTGGACCTGA AGAAACTTTC CCTGGAGAAC GTCGAGCTGC TGCAAAAAAT GGTGCCCACG 
GATGCCGAGG TCAAATCCTA CAAGGAATAT ATCATCGAGC GCAAGGACCA ACAGCTACTC 
ACCGAAGAAG ACAAGTTTAT GCTGCAGTTG TCGCGTGTGG AGCGTATCTC GTCCAAGCTA 
GCCATTATGA ACTATATGGG CAATTTTGTC GACAGCGTTC ATCTCATTAG TCCGCAAGTG 
CAATCGATAG CAGGAGCGTC GACTTCCTTA AAACAATCTC GAAAATTCAA GGCGGTTTTG 
GAAATTGTCC TGGCTTTCGG CAACTATCTC AACAGCAACA AACGGGGACC AGCCTATGGC 
TTTAAGCTGC AATCGCTGGA CACGCTGATC GATACAAAAT CCACAGACAA GCGATCGTCA 
CTGCTTCACT ATATTGTGGC CACCATACGG GCCAAATTTC CGGAGCTGCT GAACTTCGAG 
AGCGAGCTGT ATGGAACAGA CAAGGCTGCA TCGGTGGCAC TAGAGAATGT GGTGGCCGAT 
GTTCAGGAGC TTGAAAAGGG CATGGATCTG GTGCGCAAGG AGGCCGAGCT GCGAGTGAAG 
GGTGCCCAGA CGCATATCCT GCGTGACTTC CTGAACAACA GCGAGGACAA GCTGAAGAAG 
ATCAAGAGCG ATCTGCGGCA TGCACAGGAA GCGTTCAAGG AGTGCGTTGA GTACTTTGGC 
GACTCCTCGC GGAATGCAGA TGCGGCTGCT TTCTTTGCGT TGATCGTACG CTTCACGAGA 
GCGTTTAAGC AACACGATCA GGAGAACGAG CAGCGTCTTC GCCTGGAAAA GGCCGCTGCG 
CTGGCCGCTT CCAAGAAAGA GAACGATCAG GTGCTTATGC GCAACAAGGT TAACCAGAAG 
AAGCAACAGT TGCCCTCAAA CGGCGCATTA TCCTTGCAGG AAGCTGTCAT AAACGAGCTG 
AAGAGCAAGG CGCACTCGGT GCGCGAGAAA AAGCTGCTGC AGCAGGACGA GGTGTACAAC 
GGAGCCCTGG AGGACATCCT GCTCGGCCTG AAGAGCGAGC CGTACAGGCG GGCGGATGCT 
GTGCGGCGGT CGCAGCGCCG GAGGATCGAC AATAATCGTT TATCGCGCAC CCTGGAGGAA 
ATGGATTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Frl ( NM_168576.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729955.2 Drosophila melanogaster Formin-like (Frl), transcript variant B, mRNA.

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CloneID	OFa18477
Clone ID Related Accession (Same CDS sequence)	NM_168576.4
Accession Version	NM_168576.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Formin-like, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Oct 19, 2015 this sequence version replaced NM_168576.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGCCG TCAAATCGCG AACCATCACC AGCGCTGATG TGGACGCCGA TGAGCAGCTG 
CAGCATCCCC ATTCGCATGC GCACCATCAC TCGATGCGCA ATGGCCACCA GCACAACGGT 
TCCATCTCTA GCGGCACGCT GCAGAAACAG GATCTGCGCT ACGACATCGG CTGCAGTTCC 
CAGTACCAAC ATGTCAGGCA GCCGAGCCTG CGGAGCCGGA GCCAGCAACC CATGCCCACC 
ACAGATGAGC TGGACCGACG ATTCGCCAAA GTGCTGGCGT CGATGGACTT GCCACCGGAT 
AAAGCAAAGT TGCTGCGGAA CTATGATGAC GAGAAGAAAT GGGACATGAT ATGCGATCAA 
GAAATGGTGC AGGCAAAGGA TCCGCCCTCA CATTACTTGA GTAAACTGCG CACATATCTG 
GACCCAAAGG CATCAAGGAG TCATCGGAAA CGGAAAATGG TCGGCGAGTC CACGTCCACC 
CAGGTGCTCC GCGATCTGGA GATCTCGCTG CGCACGAACC ACATCGAGTG GGTGAAGGAG 
TTCCTGGATG ACACGAACCA GGGTCTGGAC GCCCTGGTCG ACTATCTCAG CTTCCGACTG 
CAGATGATGC GACACGAGCA GCGCCTTCAG GGTGTCTTGT GTGCCTCGGA GGAGCGTCTG 
AATCTCACAA ACGGCGGCGA TGGCGGTGAG ATAGTGATGG GAAACAGTAG TTCTGTTAGT 
CCTGGTGGAG GTGGTGGTTT ACTATCACAT GGAAACAGTA CGGGACATGG TCTGGCCAAT 
GGCACACTTG ACTCGAGGCA GCAGCACACA ATGTCCTATG GATTCCTACG ACCTACCATT 
GCCGATGCTC TGGATAGTCC TAGTTTGAAG CGAAGGTCAC GACATATTGC CAAATTAAAC 
ATGGGTGCCG CCACGGACGA CATCCATGTG TCCATTATGT GCCTGCGAGC TATCATGAAC 
AATAAGTATG GGTTCAACAT GGTTATCCAG CATCGCGAGG CCATCAACTG CATTGCCTTG 
AGTCTTATCC ACAAATCGCT GAGGACGAAA GCCCTGGTCC TGGAGCTGCT GGCAGCCATC 
TGTCTGGTAA AGGGAGGACA CGAAATCATT TTGGGTTCGT TCGATAATTT TAAGGATGTG 
TGCCAGGAGA AGCGACGCTT CCAAACGCTC ATGGAGTACT TTATGAACTT CGAGGCCTTT 
AACATAGATT TTATGGTTGC CTGCATGCAG TTCATGAACA TCGTTGTCCA CTCGGTGGAG 
GACATGAACT ACAGGGTGCA CTTACAGTAC GAGTTTACAG CCCTGGGCTT GGATAAGTAT 
CTGGAGCGAA TTCGATTGAC AGAATCGGAG GAACTGAAGG TGCAGATATC AGCCTATTTG 
GACAACGTCT TTGATGTTGC TGCCTTGATG GAGGATTCCG AGACAAAAAC TTCAGCCCTG 
GAACGAGTCC AAGAGCTTGA GGATCAACTT GAGCGAGAAA TAGATCGTAA CTCAGAGTTC 
CTCTATAAGT ATGCGGAATT AGAGTCCGAG AGTCTAACGC TGAAAACGGA ACGCGAGCAG 
CTGGCTATGA TTCGGCAGAA GCTGGAGGAG GAACTTACAG TGATGCAGCG AATGTTGCAG 
CACAACGAGC AGGAGCTGAA GAAACGGGAC ACACTGCTGC ACACAAAGAA CATGGAGCTG 
CAGACGCTTT CGCGTTCCCT GCCACGATCC GCCTCCAGCG GCGATGGTTC TCTGGCGAAT 
GGTGGCCTCA TGGCTGGTTC CACATCGGGG GCAGCCTCTC TAACATTGCC ACCACCTCCG 
CCGCCAATGC CCGCCTCGCC TACTGCAAGT TCAGCTGCTC CTCCACCACC TCCGCCGCCA 
GCACCACCGG CTCCACCACC ACCGCCGGGC TTCAGTCCGC TGGGCAGTCC GAGCGGCAGC 
CTAGCCTCGA CAGCGCCATC GCCGCCACAT GCCCCGCCCA TGCTAAGCTC CTTCCAACCG 
CCACCGCCTC CAGTGGCCGG CTTTATGCCC GCTCCCGATG GCGCCATGAC CATCAAACGC 
AAGGTGCCCA CTAAATACAA GTTGCCCACC TTGAACTGGA TAGCACTAAA GCCTAATCAG 
GTACGTGGTA CAATATTCAA CGAGCTGGAT GACGAAAAGA TCTTCAAGCA AATCGACTTC 
AATGAGTTTG AGGAGCGCTT CAAGATCGGG ATTGGCGGTG CTTTGCGCAA TGGTAGCAAT 
GGAACCGAGG TCGATGGGTC GCTGCAGTCC AGCAAACGCT TCAAGAGGCC CGACAATGTC 
TCGCTGCTGG AGCACACGAG GTTAAGAAAC ATTGCAATCT CCCGTCGCAA GCTGGGTATG 
CCCATTGATG ATGTCATCGC CGCCATTCAT AGTCTGGACC TGAAGAAACT TTCCCTGGAG 
AACGTCGAGC TGCTGCAAAA AATGGTGCCC ACGGATGCCG AGGTCAAATC CTACAAGGAA 
TATATCATCG AGCGCAAGGA CCAACAGCTA CTCACCGAAG AAGACAAGTT TATGCTGCAG 
TTGTCGCGTG TGGAGCGTAT CTCGTCCAAG CTAGCCATTA TGAACTATAT GGGCAATTTT 
GTCGACAGCG TTCATCTCAT TAGTCCGCAA GTGCAATCGA TAGCAGGAGC GTCGACTTCC 
TTAAAACAAT CTCGAAAATT CAAGGCGGTT TTGGAAATTG TCCTGGCTTT CGGCAACTAT 
CTCAACAGCA ACAAACGGGG ACCAGCCTAT GGCTTTAAGC TGCAATCGCT GGACACGCTG 
ATCGATACAA AATCCACAGA CAAGCGATCG TCACTGCTTC ACTATATTGT GGCCACCATA 
CGGGCCAAAT TTCCGGAGCT GCTGAACTTC GAGAGCGAGC TGTATGGAAC AGACAAGGCT 
GCATCGGTGG CACTAGAGAA TGTGGTGGCC GATGTTCAGG AGCTTGAAAA GGGCATGGAT 
CTGGTGCGCA AGGAGGCCGA GCTGCGAGTG AAGGGTGCCC AGACGCATAT CCTGCGTGAC 
TTCCTGAACA ACAGCGAGGA CAAGCTGAAG AAGATCAAGA GCGATCTGCG GCATGCACAG 
GAAGCGTTCA AGGAGTGCGT TGAGTACTTT GGCGACTCCT CGCGGAATGC AGATGCGGCT 
GCTTTCTTTG CGTTGATCGT ACGCTTCACG AGAGCGTTTA AGCAACACGA TCAGGAGAAC 
GAGCAGCGTC TTCGCCTGGA AAAGGCCGCT GCGCTGGCCG CTTCCAAGAA AGAGAACGAT 
CAGGTGCTTA TGCGCAACAA GGTTAACCAG AAGAAGCAAC AGGAAGCTGT CATAAACGAG 
CTGAAGAGCA AGGCGCACTC GGTGCGCGAG AAAAAGCTGC TGCAGCAGGA CGAGGTGTAC 
AACGGAGCCC TGGAGGACAT CCTGCTCGGC CTGAAGAGCG AGCCGTACAG GCGGGCGGAT 
GCTGTGCGGC GGTCGCAGCG CCGGAGGATC GACAATAATC GTTTATCGCG CACCCTGGAG 
GAAATGGATT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729955.2
CDS	854..4348
Translation	MGAVKSRTITSADVDADEQLQHPHSHAHHHSMRNGHQHNGSISSGTLQKQDLRYDIGCSSQYQHVRQPSLRSRSQQPMPTTDELDRRFAKVLASMDLPPDKAKLLRNYDDEKKWDMICDQEMVQAKDPPSHYLSKLRTYLDPKASRSHRKRKMVGESTSTQVLRDLEISLRTNHIEWVKEFLDDTNQGLDALVDYLSFRLQMMRHEQRLQGVLCASEERLNLTNGGDGGEIVMGNSSSVSPGGGGGLLSHGNSTGHGLANGTLDSRQQHTMSYGFLRPTIADALDSPSLKRRSRHIAKLNMGAATDDIHVSIMCLRAIMNNKYGFNMVIQHREAINCIALSLIHKSLRTKALVLELLAAICLVKGGHEIILGSFDNFKDVCQEKRRFQTLMEYFMNFEAFNIDFMVACMQFMNIVVHSVEDMNYRVHLQYEFTALGLDKYLERIRLTESEELKVQISAYLDNVFDVAALMEDSETKTSALERVQELEDQLEREIDRNSEFLYKYAELESESLTLKTEREQLAMIRQKLEEELTVMQRMLQHNEQELKKRDTLLHTKNMELQTLSRSLPRSASSGDGSLANGGLMAGSTSGAASLTLPPPPPPMPASPTASSAAPPPPPPPAPPAPPPPPGFSPLGSPSGSLASTAPSPPHAPPMLSSFQPPPPPVAGFMPAPDGAMTIKRKVPTKYKLPTLNWIALKPNQVRGTIFNELDDEKIFKQIDFNEFEERFKIGIGGALRNGSNGTEVDGSLQSSKRFKRPDNVSLLEHTRLRNIAISRRKLGMPIDDVIAAIHSLDLKKLSLENVELLQKMVPTDAEVKSYKEYIIERKDQQLLTEEDKFMLQLSRVERISSKLAIMNYMGNFVDSVHLISPQVQSIAGASTSLKQSRKFKAVLEIVLAFGNYLNSNKRGPAYGFKLQSLDTLIDTKSTDKRSSLLHYIVATIRAKFPELLNFESELYGTDKAASVALENVVADVQELEKGMDLVRKEAELRVKGAQTHILRDFLNNSEDKLKKIKSDLRHAQEAFKECVEYFGDSSRNADAAAFFALIVRFTRAFKQHDQENEQRLRLEKAAALAASKKENDQVLMRNKVNQKKQQEAVINELKSKAHSVREKKLLQQDEVYNGALEDILLGLKSEPYRRADAVRRSQRRRIDNNRLSRTLEEMDC

Target ORF information:

RefSeq Version	NM_168576.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Formin-like (Frl), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168576.4

ORF Insert Sequence:

ATGGGCGCCG TCAAATCGCG AACCATCACC AGCGCTGATG TGGACGCCGA TGAGCAGCTG 
CAGCATCCCC ATTCGCATGC GCACCATCAC TCGATGCGCA ATGGCCACCA GCACAACGGT 
TCCATCTCTA GCGGCACGCT GCAGAAACAG GATCTGCGCT ACGACATCGG CTGCAGTTCC 
CAGTACCAAC ATGTCAGGCA GCCGAGCCTG CGGAGCCGGA GCCAGCAACC CATGCCCACC 
ACAGATGAGC TGGACCGACG ATTCGCCAAA GTGCTGGCGT CGATGGACTT GCCACCGGAT 
AAAGCAAAGT TGCTGCGGAA CTATGATGAC GAGAAGAAAT GGGACATGAT ATGCGATCAA 
GAAATGGTGC AGGCAAAGGA TCCGCCCTCA CATTACTTGA GTAAACTGCG CACATATCTG 
GACCCAAAGG CATCAAGGAG TCATCGGAAA CGGAAAATGG TCGGCGAGTC CACGTCCACC 
CAGGTGCTCC GCGATCTGGA GATCTCGCTG CGCACGAACC ACATCGAGTG GGTGAAGGAG 
TTCCTGGATG ACACGAACCA GGGTCTGGAC GCCCTGGTCG ACTATCTCAG CTTCCGACTG 
CAGATGATGC GACACGAGCA GCGCCTTCAG GGTGTCTTGT GTGCCTCGGA GGAGCGTCTG 
AATCTCACAA ACGGCGGCGA TGGCGGTGAG ATAGTGATGG GAAACAGTAG TTCTGTTAGT 
CCTGGTGGAG GTGGTGGTTT ACTATCACAT GGAAACAGTA CGGGACATGG TCTGGCCAAT 
GGCACACTTG ACTCGAGGCA GCAGCACACA ATGTCCTATG GATTCCTACG ACCTACCATT 
GCCGATGCTC TGGATAGTCC TAGTTTGAAG CGAAGGTCAC GACATATTGC CAAATTAAAC 
ATGGGTGCCG CCACGGACGA CATCCATGTG TCCATTATGT GCCTGCGAGC TATCATGAAC 
AATAAGTATG GGTTCAACAT GGTTATCCAG CATCGCGAGG CCATCAACTG CATTGCCTTG 
AGTCTTATCC ACAAATCGCT GAGGACGAAA GCCCTGGTCC TGGAGCTGCT GGCAGCCATC 
TGTCTGGTAA AGGGAGGACA CGAAATCATT TTGGGTTCGT TCGATAATTT TAAGGATGTG 
TGCCAGGAGA AGCGACGCTT CCAAACGCTC ATGGAGTACT TTATGAACTT CGAGGCCTTT 
AACATAGATT TTATGGTTGC CTGCATGCAG TTCATGAACA TCGTTGTCCA CTCGGTGGAG 
GACATGAACT ACAGGGTGCA CTTACAGTAC GAGTTTACAG CCCTGGGCTT GGATAAGTAT 
CTGGAGCGAA TTCGATTGAC AGAATCGGAG GAACTGAAGG TGCAGATATC AGCCTATTTG 
GACAACGTCT TTGATGTTGC TGCCTTGATG GAGGATTCCG AGACAAAAAC TTCAGCCCTG 
GAACGAGTCC AAGAGCTTGA GGATCAACTT GAGCGAGAAA TAGATCGTAA CTCAGAGTTC 
CTCTATAAGT ATGCGGAATT AGAGTCCGAG AGTCTAACGC TGAAAACGGA ACGCGAGCAG 
CTGGCTATGA TTCGGCAGAA GCTGGAGGAG GAACTTACAG TGATGCAGCG AATGTTGCAG 
CACAACGAGC AGGAGCTGAA GAAACGGGAC ACACTGCTGC ACACAAAGAA CATGGAGCTG 
CAGACGCTTT CGCGTTCCCT GCCACGATCC GCCTCCAGCG GCGATGGTTC TCTGGCGAAT 
GGTGGCCTCA TGGCTGGTTC CACATCGGGG GCAGCCTCTC TAACATTGCC ACCACCTCCG 
CCGCCAATGC CCGCCTCGCC TACTGCAAGT TCAGCTGCTC CTCCACCACC TCCGCCGCCA 
GCACCACCGG CTCCACCACC ACCGCCGGGC TTCAGTCCGC TGGGCAGTCC GAGCGGCAGC 
CTAGCCTCGA CAGCGCCATC GCCGCCACAT GCCCCGCCCA TGCTAAGCTC CTTCCAACCG 
CCACCGCCTC CAGTGGCCGG CTTTATGCCC GCTCCCGATG GCGCCATGAC CATCAAACGC 
AAGGTGCCCA CTAAATACAA GTTGCCCACC TTGAACTGGA TAGCACTAAA GCCTAATCAG 
GTACGTGGTA CAATATTCAA CGAGCTGGAT GACGAAAAGA TCTTCAAGCA AATCGACTTC 
AATGAGTTTG AGGAGCGCTT CAAGATCGGG ATTGGCGGTG CTTTGCGCAA TGGTAGCAAT 
GGAACCGAGG TCGATGGGTC GCTGCAGTCC AGCAAACGCT TCAAGAGGCC CGACAATGTC 
TCGCTGCTGG AGCACACGAG GTTAAGAAAC ATTGCAATCT CCCGTCGCAA GCTGGGTATG 
CCCATTGATG ATGTCATCGC CGCCATTCAT AGTCTGGACC TGAAGAAACT TTCCCTGGAG 
AACGTCGAGC TGCTGCAAAA AATGGTGCCC ACGGATGCCG AGGTCAAATC CTACAAGGAA 
TATATCATCG AGCGCAAGGA CCAACAGCTA CTCACCGAAG AAGACAAGTT TATGCTGCAG 
TTGTCGCGTG TGGAGCGTAT CTCGTCCAAG CTAGCCATTA TGAACTATAT GGGCAATTTT 
GTCGACAGCG TTCATCTCAT TAGTCCGCAA GTGCAATCGA TAGCAGGAGC GTCGACTTCC 
TTAAAACAAT CTCGAAAATT CAAGGCGGTT TTGGAAATTG TCCTGGCTTT CGGCAACTAT 
CTCAACAGCA ACAAACGGGG ACCAGCCTAT GGCTTTAAGC TGCAATCGCT GGACACGCTG 
ATCGATACAA AATCCACAGA CAAGCGATCG TCACTGCTTC ACTATATTGT GGCCACCATA 
CGGGCCAAAT TTCCGGAGCT GCTGAACTTC GAGAGCGAGC TGTATGGAAC AGACAAGGCT 
GCATCGGTGG CACTAGAGAA TGTGGTGGCC GATGTTCAGG AGCTTGAAAA GGGCATGGAT 
CTGGTGCGCA AGGAGGCCGA GCTGCGAGTG AAGGGTGCCC AGACGCATAT CCTGCGTGAC 
TTCCTGAACA ACAGCGAGGA CAAGCTGAAG AAGATCAAGA GCGATCTGCG GCATGCACAG 
GAAGCGTTCA AGGAGTGCGT TGAGTACTTT GGCGACTCCT CGCGGAATGC AGATGCGGCT 
GCTTTCTTTG CGTTGATCGT ACGCTTCACG AGAGCGTTTA AGCAACACGA TCAGGAGAAC 
GAGCAGCGTC TTCGCCTGGA AAAGGCCGCT GCGCTGGCCG CTTCCAAGAA AGAGAACGAT 
CAGGTGCTTA TGCGCAACAA GGTTAACCAG AAGAAGCAAC AGGAAGCTGT CATAAACGAG 
CTGAAGAGCA AGGCGCACTC GGTGCGCGAG AAAAAGCTGC TGCAGCAGGA CGAGGTGTAC 
AACGGAGCCC TGGAGGACAT CCTGCTCGGC CTGAAGAGCG AGCCGTACAG GCGGGCGGAT 
GCTGTGCGGC GGTCGCAGCG CCGGAGGATC GACAATAATC GTTTATCGCG CACCCTGGAG 
GAAATGGATT GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Frl ( NM_001274895.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261824.1 Drosophila melanogaster Formin-like (Frl), transcript variant D, mRNA.

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CloneID	OFa20936
Clone ID Related Accession (Same CDS sequence)	NM_001274895.2
Accession Version	NM_001274895.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Formin-like, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Oct 19, 2015 this sequence version replaced NM_001274895.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGCCG TCAAATCGCG AACCATCACC AGCGCTGATG TGGACGCCGA TGAGCAGCTG 
CAGCATCCCC ATTCGCATGC GCACCATCAC TCGATGCGCA ATGGCCACCA GCACAACGGT 
TCCATCTCTA GCGGCACGCT GCAGAAACAG GATCTGCGCT ACGACATCGG CTGCAGTTCC 
CAGTACCAAC ATGTCAGGCA GCCGAGCCTG CGGAGCCGGA GCCAGCAACC CATGCCCACC 
ACAGATGAGC TGGACCGACG ATTCGCCAAA GTGCTGGCGT CGATGGACTT GCCACCGGAT 
AAAGCAAAGT TGCTGCGGAA CTATGATGAC GAGAAGAAAT GGGACATGAT ATGCGATCAA 
GAAATGGTGC AGGCAAAGGA TCCGCCCTCA CATTACTTGA GTAAACTGCG CACATATCTG 
GACCCAAAGG CATCAAGGAG TCATCGGAAA CGGAAAATGG TCGGCGAGTC CACGTCCACC 
CAGGTGCTCC GCGATCTGGA GATCTCGCTG CGCACGAACC ACATCGAGTG GGTGAAGGAG 
TTCCTGGATG ACACGAACCA GGGTCTGGAC GCCCTGGTCG ACTATCTCAG CTTCCGACTG 
CAGATGATGC GACACGAGCA GCGCCTTCAG GGTGTCTTGT GTGCCTCGGA GGAGCGTCTG 
AATCTCACAA ACGGCGGCGA TGGCGGTGAG ATAGTGATGG GAAACAGTAG TTCTGTTAGT 
CCTGGTGGAG GTGGTGGTTT ACTATCACAT GGAAACAGTA CGGGACATGG TCTGGCCAAT 
GGCACACTTG ACTCGAGGCA GCAGCACACA ATGTCCTATG GATTCCTACG ACCTACCATT 
GCCGATGCTC TGGATAGTCC TAGTTTGAAG CGAAGGTCAC GACATATTGC CAAATTAAAC 
ATGGGTGCCG CCACGGACGA CATCCATGTG TCCATTATGT GCCTGCGAGC TATCATGAAC 
AATAAGTATG GGTTCAACAT GGTTATCCAG CATCGCGAGG CCATCAACTG CATTGCCTTG 
AGTCTTATCC ACAAATCGCT GAGGACGAAA GCCCTGGTCC TGGAGCTGCT GGCAGCCATC 
TGTCTGGTAA AGGGAGGACA CGAAATCATT TTGGGTTCGT TCGATAATTT TAAGGATGTG 
TGCCAGGAGA AGCGACGCTT CCAAACGCTC ATGGAGTACT TTATGAACTT CGAGGCCTTT 
AACATAGATT TTATGGTTGC CTGCATGCAG TTCATGAACA TCGTTGTCCA CTCGGTGGAG 
GACATGAACT ACAGGGTGCA CTTACAGTAC GAGTTTACAG CCCTGGGCTT GGATAAGTAT 
CTGGAGCGAA TTCGATTGAC AGAATCGGAG GAACTGAAGG TGCAGATATC AGCCTATTTG 
GACAACGTCT TTGATGTTGC TGCCTTGATG GAGGATTCCG AGACAAAAAC TTCAGCCCTG 
GAACGAGTCC AAGAGCTTGA GGATCAACTT GAGCGAGAAA TAGATCGTAA CTCAGAGTTC 
CTCTATAAGT ATGCGGAATT AGAGTCCGAG AGTCTAACGC TGAAAACGGA ACGCGAGCAG 
CTGGCTATGA TTCGGCAGAA GCTGGAGGAG GAACTTACAG TGATGCAGCG AATGTTGCAG 
CACAACGAGC AGGAGCTGAA GAAACGGGAC ACACTGCTGC ACACAAAGAA CATGGAGCTG 
CAGACGCTTT CGCGTTCCCT GCCACGATCC GCCTCCAGCG GCGATGGTTC TCTGGCGAAT 
GGTGGCCTCA TGGCTGGTTC CACATCGGGG GCAGCCTCTC TAACATTGCC ACCACCTCCG 
CCGCCAATGC CCGCCTCGCC TACTGCAAGT TCAGCTGCTC CTCCACCACC TCCGCCGCCA 
GCACCACCGG CTCCACCACC ACCGCCGGGC TTCAGTCCGC TGGGCAGTCC GAGCGGCAGC 
CTAGCCTCGA CAGCGCCATC GCCGCCACAT GCCCCGCCCA TGCTAAGCTC CTTCCAACCG 
CCACCGCCTC CAGTGGCCGG CTTTATGCCC GCTCCCGATG GCGCCATGAC CATCAAACGC 
AAGGTGCCCA CTAAATACAA GTTGCCCACC TTGAACTGGA TAGCACTAAA GCCTAATCAG 
GTACGTGGTA CAATATTCAA CGAGCTGGAT GACGAAAAGA TCTTCAAGCA AATCGACTTC 
AATGAGTTTG AGGAGCGCTT CAAGATCGGG ATTGGCGGTG CTTTGCGCAA TGGTAGCAAT 
GGAACCGAGG TCGATGGGTC GCTGCAGTCC AGCAAACGCT TCAAGAGGCC CGACAATGTC 
TCGCTGCTGG AGCACACGAG GTTAAGAAAC ATTGCAATCT CCCGTCGCAA GCTGGGTATG 
CCCATTGATG ATGTCATCGC CGCCATTCAT AGTCTGGACC TGAAGAAACT TTCCCTGGAG 
AACGTCGAGC TGCTGCAAAA AATGGTGCCC ACGGATGCCG AGGTCAAATC CTACAAGGAA 
TATATCATCG AGCGCAAGGA CCAACAGCTA CTCACCGAAG AAGACAAGTT TATGCTGCAG 
TTGTCGCGTG TGGAGCGTAT CTCGTCCAAG CTAGCCATTA TGAACTATAT GGGCAATTTT 
GTCGACAGCG TTCATCTCAT TAGTCCGCAA GTGCAATCGA TAGCAGGAGC GTCGACTTCC 
TTAAAACAAT CTCGAAAATT CAAGGCGGTT TTGGAAATTG TCCTGGCTTT CGGCAACTAT 
CTCAACAGCA ACAAACGGGG ACCAGCCTAT GGCTTTAAGC TGCAATCGCT GGACACGCTG 
ATCGATACAA AATCCACAGA CAAGCGATCG TCACTGCTTC ACTATATTGT GGCCACCATA 
CGGGCCAAAT TTCCGGAGCT GCTGAACTTC GAGAGCGAGC TGTATGGAAC AGACAAGGCT 
GCATCGGTGG CACTAGAGAA TGTGGTGGCC GATGTTCAGG AGCTTGAAAA GGGCATGGAT 
CTGGTGCGCA AGGAGGCCGA GCTGCGAGTG AAGGGTGCCC AGACGCATAT CCTGCGTGAC 
TTCCTGAACA ACAGCGAGGA CAAGCTGAAG AAGATCAAGA GCGATCTGCG GCATGCACAG 
GAAGCGTTCA AGGAGTGCGT TGAGTACTTT GGCGACTCCT CGCGGAATGC AGATGCGGCT 
GCTTTCTTTG CGTTGATCGT ACGCTTCACG AGAGCGTTTA AGCAACACGA TCAGGAGAAC 
GAGCAGCGTC TTCGCCTGGA AAAGGCCGCT GCGCTGGCCG CTTCCAAGAA AGAGAACGAT 
CAGGTGCTTA TGCGCAACAA GGTTAACCAG AAGAAGCAAC AGCAAATACA TTGTCTGCTC 
GACTTTGCCA ATCAGCAACA GAAGCAGCTT GCCCAATCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261824.1
CDS	854..4195
Translation	MGAVKSRTITSADVDADEQLQHPHSHAHHHSMRNGHQHNGSISSGTLQKQDLRYDIGCSSQYQHVRQPSLRSRSQQPMPTTDELDRRFAKVLASMDLPPDKAKLLRNYDDEKKWDMICDQEMVQAKDPPSHYLSKLRTYLDPKASRSHRKRKMVGESTSTQVLRDLEISLRTNHIEWVKEFLDDTNQGLDALVDYLSFRLQMMRHEQRLQGVLCASEERLNLTNGGDGGEIVMGNSSSVSPGGGGGLLSHGNSTGHGLANGTLDSRQQHTMSYGFLRPTIADALDSPSLKRRSRHIAKLNMGAATDDIHVSIMCLRAIMNNKYGFNMVIQHREAINCIALSLIHKSLRTKALVLELLAAICLVKGGHEIILGSFDNFKDVCQEKRRFQTLMEYFMNFEAFNIDFMVACMQFMNIVVHSVEDMNYRVHLQYEFTALGLDKYLERIRLTESEELKVQISAYLDNVFDVAALMEDSETKTSALERVQELEDQLEREIDRNSEFLYKYAELESESLTLKTEREQLAMIRQKLEEELTVMQRMLQHNEQELKKRDTLLHTKNMELQTLSRSLPRSASSGDGSLANGGLMAGSTSGAASLTLPPPPPPMPASPTASSAAPPPPPPPAPPAPPPPPGFSPLGSPSGSLASTAPSPPHAPPMLSSFQPPPPPVAGFMPAPDGAMTIKRKVPTKYKLPTLNWIALKPNQVRGTIFNELDDEKIFKQIDFNEFEERFKIGIGGALRNGSNGTEVDGSLQSSKRFKRPDNVSLLEHTRLRNIAISRRKLGMPIDDVIAAIHSLDLKKLSLENVELLQKMVPTDAEVKSYKEYIIERKDQQLLTEEDKFMLQLSRVERISSKLAIMNYMGNFVDSVHLISPQVQSIAGASTSLKQSRKFKAVLEIVLAFGNYLNSNKRGPAYGFKLQSLDTLIDTKSTDKRSSLLHYIVATIRAKFPELLNFESELYGTDKAASVALENVVADVQELEKGMDLVRKEAELRVKGAQTHILRDFLNNSEDKLKKIKSDLRHAQEAFKECVEYFGDSSRNADAAAFFALIVRFTRAFKQHDQENEQRLRLEKAAALAASKKENDQVLMRNKVNQKKQQQIHCLLDFANQQQKQLAQS

Target ORF information:

RefSeq Version	NM_001274895.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Formin-like (Frl), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274895.2

ORF Insert Sequence:

ATGGGCGCCG TCAAATCGCG AACCATCACC AGCGCTGATG TGGACGCCGA TGAGCAGCTG 
CAGCATCCCC ATTCGCATGC GCACCATCAC TCGATGCGCA ATGGCCACCA GCACAACGGT 
TCCATCTCTA GCGGCACGCT GCAGAAACAG GATCTGCGCT ACGACATCGG CTGCAGTTCC 
CAGTACCAAC ATGTCAGGCA GCCGAGCCTG CGGAGCCGGA GCCAGCAACC CATGCCCACC 
ACAGATGAGC TGGACCGACG ATTCGCCAAA GTGCTGGCGT CGATGGACTT GCCACCGGAT 
AAAGCAAAGT TGCTGCGGAA CTATGATGAC GAGAAGAAAT GGGACATGAT ATGCGATCAA 
GAAATGGTGC AGGCAAAGGA TCCGCCCTCA CATTACTTGA GTAAACTGCG CACATATCTG 
GACCCAAAGG CATCAAGGAG TCATCGGAAA CGGAAAATGG TCGGCGAGTC CACGTCCACC 
CAGGTGCTCC GCGATCTGGA GATCTCGCTG CGCACGAACC ACATCGAGTG GGTGAAGGAG 
TTCCTGGATG ACACGAACCA GGGTCTGGAC GCCCTGGTCG ACTATCTCAG CTTCCGACTG 
CAGATGATGC GACACGAGCA GCGCCTTCAG GGTGTCTTGT GTGCCTCGGA GGAGCGTCTG 
AATCTCACAA ACGGCGGCGA TGGCGGTGAG ATAGTGATGG GAAACAGTAG TTCTGTTAGT 
CCTGGTGGAG GTGGTGGTTT ACTATCACAT GGAAACAGTA CGGGACATGG TCTGGCCAAT 
GGCACACTTG ACTCGAGGCA GCAGCACACA ATGTCCTATG GATTCCTACG ACCTACCATT 
GCCGATGCTC TGGATAGTCC TAGTTTGAAG CGAAGGTCAC GACATATTGC CAAATTAAAC 
ATGGGTGCCG CCACGGACGA CATCCATGTG TCCATTATGT GCCTGCGAGC TATCATGAAC 
AATAAGTATG GGTTCAACAT GGTTATCCAG CATCGCGAGG CCATCAACTG CATTGCCTTG 
AGTCTTATCC ACAAATCGCT GAGGACGAAA GCCCTGGTCC TGGAGCTGCT GGCAGCCATC 
TGTCTGGTAA AGGGAGGACA CGAAATCATT TTGGGTTCGT TCGATAATTT TAAGGATGTG 
TGCCAGGAGA AGCGACGCTT CCAAACGCTC ATGGAGTACT TTATGAACTT CGAGGCCTTT 
AACATAGATT TTATGGTTGC CTGCATGCAG TTCATGAACA TCGTTGTCCA CTCGGTGGAG 
GACATGAACT ACAGGGTGCA CTTACAGTAC GAGTTTACAG CCCTGGGCTT GGATAAGTAT 
CTGGAGCGAA TTCGATTGAC AGAATCGGAG GAACTGAAGG TGCAGATATC AGCCTATTTG 
GACAACGTCT TTGATGTTGC TGCCTTGATG GAGGATTCCG AGACAAAAAC TTCAGCCCTG 
GAACGAGTCC AAGAGCTTGA GGATCAACTT GAGCGAGAAA TAGATCGTAA CTCAGAGTTC 
CTCTATAAGT ATGCGGAATT AGAGTCCGAG AGTCTAACGC TGAAAACGGA ACGCGAGCAG 
CTGGCTATGA TTCGGCAGAA GCTGGAGGAG GAACTTACAG TGATGCAGCG AATGTTGCAG 
CACAACGAGC AGGAGCTGAA GAAACGGGAC ACACTGCTGC ACACAAAGAA CATGGAGCTG 
CAGACGCTTT CGCGTTCCCT GCCACGATCC GCCTCCAGCG GCGATGGTTC TCTGGCGAAT 
GGTGGCCTCA TGGCTGGTTC CACATCGGGG GCAGCCTCTC TAACATTGCC ACCACCTCCG 
CCGCCAATGC CCGCCTCGCC TACTGCAAGT TCAGCTGCTC CTCCACCACC TCCGCCGCCA 
GCACCACCGG CTCCACCACC ACCGCCGGGC TTCAGTCCGC TGGGCAGTCC GAGCGGCAGC 
CTAGCCTCGA CAGCGCCATC GCCGCCACAT GCCCCGCCCA TGCTAAGCTC CTTCCAACCG 
CCACCGCCTC CAGTGGCCGG CTTTATGCCC GCTCCCGATG GCGCCATGAC CATCAAACGC 
AAGGTGCCCA CTAAATACAA GTTGCCCACC TTGAACTGGA TAGCACTAAA GCCTAATCAG 
GTACGTGGTA CAATATTCAA CGAGCTGGAT GACGAAAAGA TCTTCAAGCA AATCGACTTC 
AATGAGTTTG AGGAGCGCTT CAAGATCGGG ATTGGCGGTG CTTTGCGCAA TGGTAGCAAT 
GGAACCGAGG TCGATGGGTC GCTGCAGTCC AGCAAACGCT TCAAGAGGCC CGACAATGTC 
TCGCTGCTGG AGCACACGAG GTTAAGAAAC ATTGCAATCT CCCGTCGCAA GCTGGGTATG 
CCCATTGATG ATGTCATCGC CGCCATTCAT AGTCTGGACC TGAAGAAACT TTCCCTGGAG 
AACGTCGAGC TGCTGCAAAA AATGGTGCCC ACGGATGCCG AGGTCAAATC CTACAAGGAA 
TATATCATCG AGCGCAAGGA CCAACAGCTA CTCACCGAAG AAGACAAGTT TATGCTGCAG 
TTGTCGCGTG TGGAGCGTAT CTCGTCCAAG CTAGCCATTA TGAACTATAT GGGCAATTTT 
GTCGACAGCG TTCATCTCAT TAGTCCGCAA GTGCAATCGA TAGCAGGAGC GTCGACTTCC 
TTAAAACAAT CTCGAAAATT CAAGGCGGTT TTGGAAATTG TCCTGGCTTT CGGCAACTAT 
CTCAACAGCA ACAAACGGGG ACCAGCCTAT GGCTTTAAGC TGCAATCGCT GGACACGCTG 
ATCGATACAA AATCCACAGA CAAGCGATCG TCACTGCTTC ACTATATTGT GGCCACCATA 
CGGGCCAAAT TTCCGGAGCT GCTGAACTTC GAGAGCGAGC TGTATGGAAC AGACAAGGCT 
GCATCGGTGG CACTAGAGAA TGTGGTGGCC GATGTTCAGG AGCTTGAAAA GGGCATGGAT 
CTGGTGCGCA AGGAGGCCGA GCTGCGAGTG AAGGGTGCCC AGACGCATAT CCTGCGTGAC 
TTCCTGAACA ACAGCGAGGA CAAGCTGAAG AAGATCAAGA GCGATCTGCG GCATGCACAG 
GAAGCGTTCA AGGAGTGCGT TGAGTACTTT GGCGACTCCT CGCGGAATGC AGATGCGGCT 
GCTTTCTTTG CGTTGATCGT ACGCTTCACG AGAGCGTTTA AGCAACACGA TCAGGAGAAC 
GAGCAGCGTC TTCGCCTGGA AAAGGCCGCT GCGCTGGCCG CTTCCAAGAA AGAGAACGAT 
CAGGTGCTTA TGCGCAACAA GGTTAACCAG AAGAAGCAAC AGCAAATACA TTGTCTGCTC 
GACTTTGCCA ATCAGCAACA GAAGCAGCTT GCCCAATCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Frl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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