ste14 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ste14
Entrez Gene ID	39478
Full Name	isoprenylcysteine carboxylmethyltransferase
Synonyms	CG11268,Dmel\CG11268,Dmel\Ste14,Ste14
General protein information	Preferred Names isoprenylcysteine carboxylmethyltransferase Names CG11268 gene product from transcript CG11268-RA CG11268-PA CG11268-PB ste14-PA ste14-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 69F5-69F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_140366.3	NP_648623.1	isoprenylcysteine carboxylmethyltransferase, isoform A
NM_001274864.1	NP_001261793.1	isoprenylcysteine carboxylmethyltransferase, isoform B

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Icmt	NP_579844.2
Xenopus tropicalis (tropical clawed frog)	icmt	XP_002933887.1
Magnaporthe oryzae (rice blast fungus)	MGG_05331	XP_003712895.1
Homo sapiens (human)	ICMT	NP_036537.1
Gallus gallus (chicken)	ICMT	NP_001006298.1
Danio rerio (zebrafish)	icmt	NP_001017618.1
Caenorhabditis elegans (roundworm)	M01E11.1	NP_491635.1
Kluyveromyces lactis	KLLA0A02167g	XP_451096.1
Mus musculus (house mouse)	Icmt	NP_598549.1
Caenorhabditis elegans (roundworm)	F21F3.3	NP_491473.1
Eremothecium gossypii	AGOS_AAR122C	NP_982664.1
Arabidopsis thaliana (thale cress)	ATSTE14B	NP_568191.1
Oryza sativa (rice)	Os04g0602900	NP_001053777.1
Pan troglodytes (chimpanzee)	ICMT	XP_525166.2
Canis lupus familiaris (dog)	ICMT	XP_546748.1
Drosophila melanogaster (fruit fly)	ste14	NP_001261793.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001310	XP_321837.3
Saccharomyces cerevisiae (baker's yeast)	STE14	NP_010698.1
Schizosaccharomyces pombe (fission yeast)	mam4	NP_593263.1
Neurospora crassa	NCU00034	XP_957021.1
Arabidopsis thaliana (thale cress)	STE14A	NP_197723.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Regulation of NOTCH signaling by RAB7 and RAB8 requires carboxyl methylation by ICMT.
Title: Regulation of NOTCH signaling by RAB7 and RAB8 requires carboxyl methylation by ICMT.

The following ste14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ste14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18389	NM_140366.3 Latest version!	Drosophila melanogaster isoprenylcysteine carboxylmethyltransferase (ste14), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa18389	NM_001274864.1 Latest version!	Drosophila melanogaster isoprenylcysteine carboxylmethyltransferase (ste14), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ste14 ( NM_140366.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648623.1 Drosophila melanogaster isoprenylcysteine carboxylmethyltransferase (ste14), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18389
Clone ID Related Accession (Same CDS sequence)	NM_140366.3 , NM_001274864.1
Accession Version	NM_140366.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	isoprenylcysteine carboxylmethyltransferase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140366.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGTGCAA AGAGCCGAGT GGCTTCTCCT GGGGCCGGTG GCACCAGCCT GTGTTCCGAA 
GGCCGCATCA GTTTATATTG CTTTCTGATT ACTGCAGCTC TAGTCCTGAT TCCCTCGGTG 
CCCCAGAATC TTTATGGAGT GGTGCCACAG GTGTGGGGCG CCGTCCTCTG GGGTCCATTT 
TTGTACTACG CCCTTATTAA CATGATCATA CGGTTTGTGC TGCGGAACCA CGACTACCAG 
GTGGCCATTA GGGCATCCTT CCTGGGCTTT GCCATGGCCG TGAGTGTGCT GGTCATCTGC 
TTTGCTCCCA CCGAGTGGCA GCAATTTGGC GCCTACGGAT GCTTCATGTC CCTTTTCCAC 
TACTCGGAGT TCCTCGTTAT CGCCTTTGCC AATCCGCGCA CTCTCAGTCT CGACTCCTTT 
ATGCTGAACC ACTCTGTGCA CTATGGACTG GCGGCGGCAG CCAGTTGGAT CGAGTTCTCA 
CTAGAGGTGT ACTACCTGCC GCAGTTCAAG AGATACGGAT ACATTTGGGT GGCGGGCGTG 
GTCCTGTGCC TGCTGGGCGA GATGGTTCGC AAGGCAGCCA TCATCACAGC TGGCCGGAGC 
TTCACTCACT TGGTCCAGAA TGAGAAACAC TCGGAACACA AGCTAATAAC AAGCGGGATC 
TACGCCTACT GCCGCCACCC GTCTTATGTC GGCTGGTTCT GGTGGTCCAT CGGAACCCAG 
ATCGTGCTAT TGAATCCCAT CTGCATATGC ATTTACACAC TGGTCAGCTG GCTGTTCTTC 
CACGATCGCA TCTACGTCGA GGAGTACTCC CTGCTCAACT TCTTCCAGTC GGACTATGTG 
CGCTATCAAA AGCGGGTGCC AACGGGCCTG CCCTTCATCC GGGGCTATCT GGTCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648623.1
CDS	116..1015
Translation	MCAKSRVASPGAGGTSLCSEGRISLYCFLITAALVLIPSVPQNLYGVVPQVWGAVLWGPFLYYALINMIIRFVLRNHDYQVAIRASFLGFAMAVSVLVICFAPTEWQQFGAYGCFMSLFHYSEFLVIAFANPRTLSLDSFMLNHSVHYGLAAAASWIEFSLEVYYLPQFKRYGYIWVAGVVLCLLGEMVRKAAIITAGRSFTHLVQNEKHSEHKLITSGIYAYCRHPSYVGWFWWSIGTQIVLLNPICICIYTLVSWLFFHDRIYVEEYSLLNFFQSDYVRYQKRVPTGLPFIRGYLVD

Target ORF information:

RefSeq Version	NM_140366.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster isoprenylcysteine carboxylmethyltransferase (ste14), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140366.3

ORF Insert Sequence:

ATGTGTGCAA AGAGCCGAGT GGCTTCTCCT GGGGCCGGTG GCACCAGCCT GTGTTCCGAA 
GGCCGCATCA GTTTATATTG CTTTCTGATT ACTGCAGCTC TAGTCCTGAT TCCCTCGGTG 
CCCCAGAATC TTTATGGAGT GGTGCCACAG GTGTGGGGCG CCGTCCTCTG GGGTCCATTT 
TTGTACTACG CCCTTATTAA CATGATCATA CGGTTTGTGC TGCGGAACCA CGACTACCAG 
GTGGCCATTA GGGCATCCTT CCTGGGCTTT GCCATGGCCG TGAGTGTGCT GGTCATCTGC 
TTTGCTCCCA CCGAGTGGCA GCAATTTGGC GCCTACGGAT GCTTCATGTC CCTTTTCCAC 
TACTCGGAGT TCCTCGTTAT CGCCTTTGCC AATCCGCGCA CTCTCAGTCT CGACTCCTTT 
ATGCTGAACC ACTCTGTGCA CTATGGACTG GCGGCGGCAG CCAGTTGGAT CGAGTTCTCA 
CTAGAGGTGT ACTACCTGCC GCAGTTCAAG AGATACGGAT ACATTTGGGT GGCGGGCGTG 
GTCCTGTGCC TGCTGGGCGA GATGGTTCGC AAGGCAGCCA TCATCACAGC TGGCCGGAGC 
TTCACTCACT TGGTCCAGAA TGAGAAACAC TCGGAACACA AGCTAATAAC AAGCGGGATC 
TACGCCTACT GCCGCCACCC GTCTTATGTC GGCTGGTTCT GGTGGTCCAT CGGAACCCAG 
ATCGTGCTAT TGAATCCCAT CTGCATATGC ATTTACACAC TGGTCAGCTG GCTGTTCTTC 
CACGATCGCA TCTACGTCGA GGAGTACTCC CTGCTCAACT TCTTCCAGTC GGACTATGTG 
CGCTATCAAA AGCGGGTGCC AACGGGCCTG CCCTTCATCC GGGGCTATCT GGTCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ste14 ( NM_001274864.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261793.1 Drosophila melanogaster isoprenylcysteine carboxylmethyltransferase (ste14), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18389
Clone ID Related Accession (Same CDS sequence)	NM_140366.3 , NM_001274864.1
Accession Version	NM_001274864.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	isoprenylcysteine carboxylmethyltransferase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGTGCAA AGAGCCGAGT GGCTTCTCCT GGGGCCGGTG GCACCAGCCT GTGTTCCGAA 
GGCCGCATCA GTTTATATTG CTTTCTGATT ACTGCAGCTC TAGTCCTGAT TCCCTCGGTG 
CCCCAGAATC TTTATGGAGT GGTGCCACAG GTGTGGGGCG CCGTCCTCTG GGGTCCATTT 
TTGTACTACG CCCTTATTAA CATGATCATA CGGTTTGTGC TGCGGAACCA CGACTACCAG 
GTGGCCATTA GGGCATCCTT CCTGGGCTTT GCCATGGCCG TGAGTGTGCT GGTCATCTGC 
TTTGCTCCCA CCGAGTGGCA GCAATTTGGC GCCTACGGAT GCTTCATGTC CCTTTTCCAC 
TACTCGGAGT TCCTCGTTAT CGCCTTTGCC AATCCGCGCA CTCTCAGTCT CGACTCCTTT 
ATGCTGAACC ACTCTGTGCA CTATGGACTG GCGGCGGCAG CCAGTTGGAT CGAGTTCTCA 
CTAGAGGTGT ACTACCTGCC GCAGTTCAAG AGATACGGAT ACATTTGGGT GGCGGGCGTG 
GTCCTGTGCC TGCTGGGCGA GATGGTTCGC AAGGCAGCCA TCATCACAGC TGGCCGGAGC 
TTCACTCACT TGGTCCAGAA TGAGAAACAC TCGGAACACA AGCTAATAAC AAGCGGGATC 
TACGCCTACT GCCGCCACCC GTCTTATGTC GGCTGGTTCT GGTGGTCCAT CGGAACCCAG 
ATCGTGCTAT TGAATCCCAT CTGCATATGC ATTTACACAC TGGTCAGCTG GCTGTTCTTC 
CACGATCGCA TCTACGTCGA GGAGTACTCC CTGCTCAACT TCTTCCAGTC GGACTATGTG 
CGCTATCAAA AGCGGGTGCC AACGGGCCTG CCCTTCATCC GGGGCTATCT GGTCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261793.1
CDS	116..1015
Translation	MCAKSRVASPGAGGTSLCSEGRISLYCFLITAALVLIPSVPQNLYGVVPQVWGAVLWGPFLYYALINMIIRFVLRNHDYQVAIRASFLGFAMAVSVLVICFAPTEWQQFGAYGCFMSLFHYSEFLVIAFANPRTLSLDSFMLNHSVHYGLAAAASWIEFSLEVYYLPQFKRYGYIWVAGVVLCLLGEMVRKAAIITAGRSFTHLVQNEKHSEHKLITSGIYAYCRHPSYVGWFWWSIGTQIVLLNPICICIYTLVSWLFFHDRIYVEEYSLLNFFQSDYVRYQKRVPTGLPFIRGYLVD

Target ORF information:

RefSeq Version	NM_001274864.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster isoprenylcysteine carboxylmethyltransferase (ste14), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274864.1

ORF Insert Sequence:

ATGTGTGCAA AGAGCCGAGT GGCTTCTCCT GGGGCCGGTG GCACCAGCCT GTGTTCCGAA 
GGCCGCATCA GTTTATATTG CTTTCTGATT ACTGCAGCTC TAGTCCTGAT TCCCTCGGTG 
CCCCAGAATC TTTATGGAGT GGTGCCACAG GTGTGGGGCG CCGTCCTCTG GGGTCCATTT 
TTGTACTACG CCCTTATTAA CATGATCATA CGGTTTGTGC TGCGGAACCA CGACTACCAG 
GTGGCCATTA GGGCATCCTT CCTGGGCTTT GCCATGGCCG TGAGTGTGCT GGTCATCTGC 
TTTGCTCCCA CCGAGTGGCA GCAATTTGGC GCCTACGGAT GCTTCATGTC CCTTTTCCAC 
TACTCGGAGT TCCTCGTTAT CGCCTTTGCC AATCCGCGCA CTCTCAGTCT CGACTCCTTT 
ATGCTGAACC ACTCTGTGCA CTATGGACTG GCGGCGGCAG CCAGTTGGAT CGAGTTCTCA 
CTAGAGGTGT ACTACCTGCC GCAGTTCAAG AGATACGGAT ACATTTGGGT GGCGGGCGTG 
GTCCTGTGCC TGCTGGGCGA GATGGTTCGC AAGGCAGCCA TCATCACAGC TGGCCGGAGC 
TTCACTCACT TGGTCCAGAA TGAGAAACAC TCGGAACACA AGCTAATAAC AAGCGGGATC 
TACGCCTACT GCCGCCACCC GTCTTATGTC GGCTGGTTCT GGTGGTCCAT CGGAACCCAG 
ATCGTGCTAT TGAATCCCAT CTGCATATGC ATTTACACAC TGGTCAGCTG GCTGTTCTTC 
CACGATCGCA TCTACGTCGA GGAGTACTCC CTGCTCAACT TCTTCCAGTC GGACTATGTG 
CGCTATCAAA AGCGGGTGCC AACGGGCCTG CCCTTCATCC GGGGCTATCT GGTCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ste14 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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