Ent3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ent3
Entrez Gene ID	39461
Full Name	Equilibrative nucleoside transporter 3
Synonyms	CG11010,DmENT3,DmEnt3,Dmel\CG11010,ent3
General protein information	Preferred Names Equilibrative nucleoside transporter 3 Names CG11010 gene product from transcript CG11010-RC CG11010-PA CG11010-PB CG11010-PC Ent3-PA Ent3-PB Ent3-PC equilibrative nucleoside transporter 3
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 69E6-69E6

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274859.2	NP_001261788.1	equilibrative nucleoside transporter 3, isoform B
NM_001300123.1	NP_001287052.1	equilibrative nucleoside transporter 3, isoform C
NM_140351.3	NP_648608.2	equilibrative nucleoside transporter 3, isoform A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SLC29A4	XP_547001.3
Mus musculus (house mouse)	Slc29a4	NP_666369.1
Danio rerio (zebrafish)	slc29a4	NP_001074041.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011796	XP_320720.3
Arabidopsis thaliana (thale cress)	ENT1	NP_564987.2
	LOC717249	XP_001108580.1
Drosophila melanogaster (fruit fly)	Ent3	NP_001261788.1
Rattus norvegicus (Norway rat)	Slc29a4	NP_001099381.1
Homo sapiens (human)	SLC29A4	NP_001035751.1
Bos taurus (cattle)	SLC29A4	NP_001180125.1
Gallus gallus (chicken)	SLC29A4	XP_004945211.1
Oryza sativa (rice)	Os08g0205200	NP_001061232.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ent3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ent3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18383	NM_001274859.2 Latest version!	Drosophila melanogaster equilibrative nucleoside transporter 3, transcript variant B (Ent3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa20883	NM_140351.3 Latest version!	Drosophila melanogaster equilibrative nucleoside transporter 3, transcript variant A (Ent3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa20883	NM_001300123.1 Latest version!	Drosophila melanogaster equilibrative nucleoside transporter 3, transcript variant C (Ent3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ent3 ( NM_001274859.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261788.1 Drosophila melanogaster equilibrative nucleoside transporter 3, transcript variant B (Ent3), mRNA.

Price & Availability↑

CloneID	OFa18383
Clone ID Related Accession (Same CDS sequence)	NM_001274859.2
Accession Version	NM_001274859.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	equilibrative nucleoside transporter 3, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001274859.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGTTG GTGGCCAGGA AACCACCTAT GAGCCGCTGG ACGGACGTGG TTCCGGGCGT 
TCGAGGAGCA GTCATTCCAC AACCGGATCC GGCCTGAGGC GTCCAGGCAG CATGGACAGT 
CCGGAGTTCG ATAACCGTGC ACCCAAGGAT CAGAGGCACT CCGTCTACCT GGCCCTACTG 
GCAGCGGGCA TTGGTTTTGT GTTGCCCTAC AATAGTTTCA TTATTGCGGC GGACTACTGG 
CAGGCACGAT TCCCGGGTCG CCCGGTAGCC CTGGACATGT CGATGACATA CATCTTCGTT 
GCCTTCGGCA CCGTTTTGAT GAACAACATT GTGCTATCGG TGGCACCGTT CCAGTCGCGA 
GTGCTCTTTG GCTACATGAT ATCCTTCACC ACACTGATCT TTGTCGCGGT GTGCGAGGTG 
GCTTGGCATA TGTTCGCCAC AAATACCGCC TATTTGGTGA ATATGTCAGC TGTTGCCCTG 
ACCGCCATCG GATGTACTGT GCAGCAGTCG AGCTTCTATG GATTCGCCTC CATGCTGCCC 
AAGCAGTATA CCCAGGCGGT GATGGCCGGC GAGAGTATCG CCGGTTTCCT GGTGTCTTCC 
AATCGGGTCG TTACCAAATT GCTGATTAAC AACGATCGCG TGTCCACGGT GATCTTCTTT 
TTGACCTCAA CGCTCTACAT ACTCTTCAGC TACCTGTTGC ATGTGGCCAC CATTAACTCG 
CCGTTTGTGC GGTTCCATGT GGAGGCCTGC TCCAAGATCG TTTTGCGGCC GGATGAGGAG 
ATTGATGGAG TTCCAAGTAG TACTCGATAT GGTGTGCTCT CGATGGACGG TACCCACACC 
ACGACCACAT CGGTTGGACC TCCAGGCACT GGAAATACCC TTAGTTTTAG CAATCCCGTC 
TACGAGCTAT CCAACCCGAC TGCCGGCGAG AGTAGCATTG AAGCCCTCGG ACAGCTGCCC 
GAACTTCCGG CCACGCCCCC AGCTCAGGAG CCTACAACGC CCACGACGGT GGCCTTCAAG 
GTGGAACACG TTATCACGCC ACGTCGTTGC CGGCCCAGCA AGTTGGGTGA CATCCGCGAG 
GGATTCGTGA CCCGATGGCG AGTGGCCCAG GTGATCTACC CGCACATGGT GTGCATCGCC 
TTGGCCTATT GTGTGACCCT CTCGCTGTAT CCGGGTATCG AGGTTGAGGT GCAGTCATGT 
GCCCTTCGCT CCTGGATGCC CGTGCTGTTG ATGTTCTGCT TTAATACGTC GGATGTGGTT 
GGCAAGATAC TGGCCGCCAG TCCGTATCCA TGGTCGCGTC GCCAACTGAT CCTGCTGTCG 
GGACTAAGGA TTGTGCTGGT GCCGCTCCTG CTCCTCTGCT GTGCTCCCCG CCAGCGTCCC 
GTGATATCCG GCGAGACGGC GCCGTTTGTC TTCACCATCG CTCTGGGCAT CACGAATGGA 
TTGGCAGGCA GCCTGCCCAT GATGTTGGCA CCGGCAAAGG TGCCGGGAAC ACTCAAGGAG 
GTCACTGGCA ATATAATGAC CCTGTCGTAT AATGTTGGTC TGACGGTGGG CTCTCTGATT 
GGATACGTTT TCGAGAGTAT GCTGGGACCG CAGCTGGTGA ATCCGTGTCC CACATATCCC 
TATGTGCCCG CCTCGATGCT GGAACAGTTC CATGGTCACG GGCATGGACA TGGCCATCTA 
CCACATCCTC TGCCGCTGAC TAGCACCAGC ACAATGAGTC CACCAACTAC GGGGGCCACC 
ACATTGGCGC CACTACTGCT CAACACCACG CTGGCTACGC TTAGTAGCTC TAGCTCCACC 
ACCAGCACCG CCAGTCCGGC ACTGGCCACC GTTATCACAA CCACAGCTCT GGCGCTCGCC 
AGTACGGTGG CCAGCGGAAG TGCCGAGGTG ATTAATGCCA CCATATCATC GATCCTGTCC 
ACGGTCAGCA GTTCGGTGGG CGATATCAGC GACGAGATGA CCACAGATCC TCAGACGCCA 
GATGCACTGC TCGAAAATAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261788.1
CDS	404..2407
Translation	MDVGGQETTYEPLDGRGSGRSRSSHSTTGSGLRRPGSMDSPEFDNRAPKDQRHSVYLALLAAGIGFVLPYNSFIIAADYWQARFPGRPVALDMSMTYIFVAFGTVLMNNIVLSVAPFQSRVLFGYMISFTTLIFVAVCEVAWHMFATNTAYLVNMSAVALTAIGCTVQQSSFYGFASMLPKQYTQAVMAGESIAGFLVSSNRVVTKLLINNDRVSTVIFFLTSTLYILFSYLLHVATINSPFVRFHVEACSKIVLRPDEEIDGVPSSTRYGVLSMDGTHTTTTSVGPPGTGNTLSFSNPVYELSNPTAGESSIEALGQLPELPATPPAQEPTTPTTVAFKVEHVITPRRCRPSKLGDIREGFVTRWRVAQVIYPHMVCIALAYCVTLSLYPGIEVEVQSCALRSWMPVLLMFCFNTSDVVGKILAASPYPWSRRQLILLSGLRIVLVPLLLLCCAPRQRPVISGETAPFVFTIALGITNGLAGSLPMMLAPAKVPGTLKEVTGNIMTLSYNVGLTVGSLIGYVFESMLGPQLVNPCPTYPYVPASMLEQFHGHGHGHGHLPHPLPLTSTSTMSPPTTGATTLAPLLLNTTLATLSSSSSTTSTASPALATVITTTALALASTVASGSAEVINATISSILSTVSSSVGDISDEMTTDPQTPDALLENI

Target ORF information:

RefSeq Version	NM_001274859.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster equilibrative nucleoside transporter 3, transcript variant B (Ent3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274859.2

ORF Insert Sequence:

ATGGATGTTG GTGGCCAGGA AACCACCTAT GAGCCGCTGG ACGGACGTGG TTCCGGGCGT 
TCGAGGAGCA GTCATTCCAC AACCGGATCC GGCCTGAGGC GTCCAGGCAG CATGGACAGT 
CCGGAGTTCG ATAACCGTGC ACCCAAGGAT CAGAGGCACT CCGTCTACCT GGCCCTACTG 
GCAGCGGGCA TTGGTTTTGT GTTGCCCTAC AATAGTTTCA TTATTGCGGC GGACTACTGG 
CAGGCACGAT TCCCGGGTCG CCCGGTAGCC CTGGACATGT CGATGACATA CATCTTCGTT 
GCCTTCGGCA CCGTTTTGAT GAACAACATT GTGCTATCGG TGGCACCGTT CCAGTCGCGA 
GTGCTCTTTG GCTACATGAT ATCCTTCACC ACACTGATCT TTGTCGCGGT GTGCGAGGTG 
GCTTGGCATA TGTTCGCCAC AAATACCGCC TATTTGGTGA ATATGTCAGC TGTTGCCCTG 
ACCGCCATCG GATGTACTGT GCAGCAGTCG AGCTTCTATG GATTCGCCTC CATGCTGCCC 
AAGCAGTATA CCCAGGCGGT GATGGCCGGC GAGAGTATCG CCGGTTTCCT GGTGTCTTCC 
AATCGGGTCG TTACCAAATT GCTGATTAAC AACGATCGCG TGTCCACGGT GATCTTCTTT 
TTGACCTCAA CGCTCTACAT ACTCTTCAGC TACCTGTTGC ATGTGGCCAC CATTAACTCG 
CCGTTTGTGC GGTTCCATGT GGAGGCCTGC TCCAAGATCG TTTTGCGGCC GGATGAGGAG 
ATTGATGGAG TTCCAAGTAG TACTCGATAT GGTGTGCTCT CGATGGACGG TACCCACACC 
ACGACCACAT CGGTTGGACC TCCAGGCACT GGAAATACCC TTAGTTTTAG CAATCCCGTC 
TACGAGCTAT CCAACCCGAC TGCCGGCGAG AGTAGCATTG AAGCCCTCGG ACAGCTGCCC 
GAACTTCCGG CCACGCCCCC AGCTCAGGAG CCTACAACGC CCACGACGGT GGCCTTCAAG 
GTGGAACACG TTATCACGCC ACGTCGTTGC CGGCCCAGCA AGTTGGGTGA CATCCGCGAG 
GGATTCGTGA CCCGATGGCG AGTGGCCCAG GTGATCTACC CGCACATGGT GTGCATCGCC 
TTGGCCTATT GTGTGACCCT CTCGCTGTAT CCGGGTATCG AGGTTGAGGT GCAGTCATGT 
GCCCTTCGCT CCTGGATGCC CGTGCTGTTG ATGTTCTGCT TTAATACGTC GGATGTGGTT 
GGCAAGATAC TGGCCGCCAG TCCGTATCCA TGGTCGCGTC GCCAACTGAT CCTGCTGTCG 
GGACTAAGGA TTGTGCTGGT GCCGCTCCTG CTCCTCTGCT GTGCTCCCCG CCAGCGTCCC 
GTGATATCCG GCGAGACGGC GCCGTTTGTC TTCACCATCG CTCTGGGCAT CACGAATGGA 
TTGGCAGGCA GCCTGCCCAT GATGTTGGCA CCGGCAAAGG TGCCGGGAAC ACTCAAGGAG 
GTCACTGGCA ATATAATGAC CCTGTCGTAT AATGTTGGTC TGACGGTGGG CTCTCTGATT 
GGATACGTTT TCGAGAGTAT GCTGGGACCG CAGCTGGTGA ATCCGTGTCC CACATATCCC 
TATGTGCCCG CCTCGATGCT GGAACAGTTC CATGGTCACG GGCATGGACA TGGCCATCTA 
CCACATCCTC TGCCGCTGAC TAGCACCAGC ACAATGAGTC CACCAACTAC GGGGGCCACC 
ACATTGGCGC CACTACTGCT CAACACCACG CTGGCTACGC TTAGTAGCTC TAGCTCCACC 
ACCAGCACCG CCAGTCCGGC ACTGGCCACC GTTATCACAA CCACAGCTCT GGCGCTCGCC 
AGTACGGTGG CCAGCGGAAG TGCCGAGGTG ATTAATGCCA CCATATCATC GATCCTGTCC 
ACGGTCAGCA GTTCGGTGGG CGATATCAGC GACGAGATGA CCACAGATCC TCAGACGCCA 
GATGCACTGC TCGAAAATAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ent3 ( NM_001300123.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287052.1 Drosophila melanogaster equilibrative nucleoside transporter 3, transcript variant C (Ent3), mRNA.

Price & Availability↑

CloneID	OFa20883
Clone ID Related Accession (Same CDS sequence)	NM_001300123.1 , NM_140351.3
Accession Version	NM_001300123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	equilibrative nucleoside transporter 3, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGTTG GTGGCCAGGA AACCACCTAT GAGCCGCTGG ACGGACGTGG TTCCGGGCGT 
TCGAGGAGCA GTCATTCCAC AACCGGATCC GGCCTGAGGC GTCCAGGCAG CATGGACAGT 
CCGGAGTTCG ATAACCGTGC ACCCAAGGAT CAGAGGCACT CCGTCTACCT GGCCCTACTG 
GCAGCGGGCA TTGGTTTTGT GTTGCCCTAC AATAGTTTCA TTATTGCGGC GGACTACTGG 
CAGGCACGAT TCCCGGGTCG CCCGGTAGCC CTGGACATGT CGATGACATA CATCTTCGTT 
GCCTTCGGCA CCGTTTTGAT GAACAACATT GTGCTATCGG TGGCACCGTT CCAGTCGCGA 
GTGCTCTTTG GCTACATGAT ATCCTTCACC ACACTGATCT TTGTCGCGGT GTGCGAGGTG 
GCTTGGCATA TGTTCGCCAC AAATACCGCC TATTTGGTGA ATATGTCAGC TGTTGCCCTG 
ACCGCCATCG GATGTACTGT GCAGCAGTCG AGCTTCTATG GATTCGCCTC CATGCTGCCC 
AAGCAGTATA CCCAGGCGGT GATGGCCGGC GAGAGTATCG CCGGTTTCCT GGTGTCTTCC 
AATCGGGTCG TTACCAAATT GCTGATTAAC AACGATCGCG TGTCCACGGT GATCTTCTTT 
TTGACCTCAA CGCTCTACAT ACTCTTCAGC TACCTGTTGC ATGTGGCCAC CATTAACTCG 
CCGTTTGTGC GGTTCCATGT GGAGGCCTGC TCCAAGATCG TTTTGCGGCC GGATGAGCAG 
GAGATTGATG GAGTTCCAAG TAGTACTCGA TATGGTGTGC TCTCGATGGA CGGTACCCAC 
ACCACGACCA CATCGGTTGG ACCTCCAGGC ACTGGAAATA CCCTTAGTTT TAGCAATCCC 
GTCTACGAGC TATCCAACCC GACTGCCGGC GAGAGTAGCA TTGAAGCCCT CGGACAGCTG 
CCCGAACTTC CGGCCACGCC CCCAGCTCAG GAGCCTACAA CGCCCACGAC GGTGGCCTTC 
AAGGTGGAAC ACGTTATCAC GCCACGTCGT TGCCGGCCCA GCAAGTTGGG TGACATCCGC 
GAGGGATTCG TGACCCGATG GCGAGTGGCC CAGGTGATCT ACCCGCACAT GGTGTGCATC 
GCCTTGGCCT ATTGTGTGAC CCTCTCGCTG TATCCGGGTA TCGAGGTTGA GGTGCAGTCA 
TGTGCCCTTC GCTCCTGGAT GCCCGTGCTG TTGATGTTCT GCTTTAATAC GTCGGATGTG 
GTTGGCAAGA TACTGGCCGC CAGTCCGTAT CCATGGTCGC GTCGCCAACT GATCCTGCTG 
TCGGGACTAA GGATTGTGCT GGTGCCGCTC CTGCTCCTCT GCTGTGCTCC CCGCCAGCGT 
CCCGTGATAT CCGGCGAGAC GGCGCCGTTT GTCTTCACCA TCGCTCTGGG CATCACGAAT 
GGATTGGCAG GCAGCCTGCC CATGATGTTG GCACCGGCAA AGGTGCCGGG AACACTCAAG 
GAGGTCACTG GCAATATAAT GACCCTGTCG TATAATGTTG GTCTGACGGT GGGCTCTCTG 
ATTGGATACG TTTTCGAGAG TATGCTGGGA CCGCAGCTGG TGAATCCGTG TCCCACATAT 
CCCTATGTGC CCGCCTCGAT GCTGGAACAG TTCCATGGTC ACGGGCATGG ACATGGCCAT 
CTACCACATC CTCTGCCGCT GACTAGCACC AGCACAATGA GTCCACCAAC TACGGGGGCC 
ACCACATTGG CGCCACTACT GCTCAACACC ACGCTGGCTA CGCTTAGTAG CTCTAGCTCC 
ACCACCAGCA CCGCCAGTCC GGCACTGGCC ACCGTTATCA CAACCACAGC TCTGGCGCTC 
GCCAGTACGG TGGCCAGCGG AAGTGCCGAG GTGATTAATG CCACCATATC ATCGATCCTG 
TCCACGGTCA GCAGTTCGGT GGGCGATATC AGCGACGAGA TGACCACAGA TCCTCAGACG 
CCAGATGCAC TGCTCGAAAA TATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287052.1
CDS	404..2410
Translation	MDVGGQETTYEPLDGRGSGRSRSSHSTTGSGLRRPGSMDSPEFDNRAPKDQRHSVYLALLAAGIGFVLPYNSFIIAADYWQARFPGRPVALDMSMTYIFVAFGTVLMNNIVLSVAPFQSRVLFGYMISFTTLIFVAVCEVAWHMFATNTAYLVNMSAVALTAIGCTVQQSSFYGFASMLPKQYTQAVMAGESIAGFLVSSNRVVTKLLINNDRVSTVIFFLTSTLYILFSYLLHVATINSPFVRFHVEACSKIVLRPDEQEIDGVPSSTRYGVLSMDGTHTTTTSVGPPGTGNTLSFSNPVYELSNPTAGESSIEALGQLPELPATPPAQEPTTPTTVAFKVEHVITPRRCRPSKLGDIREGFVTRWRVAQVIYPHMVCIALAYCVTLSLYPGIEVEVQSCALRSWMPVLLMFCFNTSDVVGKILAASPYPWSRRQLILLSGLRIVLVPLLLLCCAPRQRPVISGETAPFVFTIALGITNGLAGSLPMMLAPAKVPGTLKEVTGNIMTLSYNVGLTVGSLIGYVFESMLGPQLVNPCPTYPYVPASMLEQFHGHGHGHGHLPHPLPLTSTSTMSPPTTGATTLAPLLLNTTLATLSSSSSTTSTASPALATVITTTALALASTVASGSAEVINATISSILSTVSSSVGDISDEMTTDPQTPDALLENI

Target ORF information:

RefSeq Version	NM_001300123.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster equilibrative nucleoside transporter 3, transcript variant C (Ent3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300123.1

ORF Insert Sequence:

ATGGATGTTG GTGGCCAGGA AACCACCTAT GAGCCGCTGG ACGGACGTGG TTCCGGGCGT 
TCGAGGAGCA GTCATTCCAC AACCGGATCC GGCCTGAGGC GTCCAGGCAG CATGGACAGT 
CCGGAGTTCG ATAACCGTGC ACCCAAGGAT CAGAGGCACT CCGTCTACCT GGCCCTACTG 
GCAGCGGGCA TTGGTTTTGT GTTGCCCTAC AATAGTTTCA TTATTGCGGC GGACTACTGG 
CAGGCACGAT TCCCGGGTCG CCCGGTAGCC CTGGACATGT CGATGACATA CATCTTCGTT 
GCCTTCGGCA CCGTTTTGAT GAACAACATT GTGCTATCGG TGGCACCGTT CCAGTCGCGA 
GTGCTCTTTG GCTACATGAT ATCCTTCACC ACACTGATCT TTGTCGCGGT GTGCGAGGTG 
GCTTGGCATA TGTTCGCCAC AAATACCGCC TATTTGGTGA ATATGTCAGC TGTTGCCCTG 
ACCGCCATCG GATGTACTGT GCAGCAGTCG AGCTTCTATG GATTCGCCTC CATGCTGCCC 
AAGCAGTATA CCCAGGCGGT GATGGCCGGC GAGAGTATCG CCGGTTTCCT GGTGTCTTCC 
AATCGGGTCG TTACCAAATT GCTGATTAAC AACGATCGCG TGTCCACGGT GATCTTCTTT 
TTGACCTCAA CGCTCTACAT ACTCTTCAGC TACCTGTTGC ATGTGGCCAC CATTAACTCG 
CCGTTTGTGC GGTTCCATGT GGAGGCCTGC TCCAAGATCG TTTTGCGGCC GGATGAGCAG 
GAGATTGATG GAGTTCCAAG TAGTACTCGA TATGGTGTGC TCTCGATGGA CGGTACCCAC 
ACCACGACCA CATCGGTTGG ACCTCCAGGC ACTGGAAATA CCCTTAGTTT TAGCAATCCC 
GTCTACGAGC TATCCAACCC GACTGCCGGC GAGAGTAGCA TTGAAGCCCT CGGACAGCTG 
CCCGAACTTC CGGCCACGCC CCCAGCTCAG GAGCCTACAA CGCCCACGAC GGTGGCCTTC 
AAGGTGGAAC ACGTTATCAC GCCACGTCGT TGCCGGCCCA GCAAGTTGGG TGACATCCGC 
GAGGGATTCG TGACCCGATG GCGAGTGGCC CAGGTGATCT ACCCGCACAT GGTGTGCATC 
GCCTTGGCCT ATTGTGTGAC CCTCTCGCTG TATCCGGGTA TCGAGGTTGA GGTGCAGTCA 
TGTGCCCTTC GCTCCTGGAT GCCCGTGCTG TTGATGTTCT GCTTTAATAC GTCGGATGTG 
GTTGGCAAGA TACTGGCCGC CAGTCCGTAT CCATGGTCGC GTCGCCAACT GATCCTGCTG 
TCGGGACTAA GGATTGTGCT GGTGCCGCTC CTGCTCCTCT GCTGTGCTCC CCGCCAGCGT 
CCCGTGATAT CCGGCGAGAC GGCGCCGTTT GTCTTCACCA TCGCTCTGGG CATCACGAAT 
GGATTGGCAG GCAGCCTGCC CATGATGTTG GCACCGGCAA AGGTGCCGGG AACACTCAAG 
GAGGTCACTG GCAATATAAT GACCCTGTCG TATAATGTTG GTCTGACGGT GGGCTCTCTG 
ATTGGATACG TTTTCGAGAG TATGCTGGGA CCGCAGCTGG TGAATCCGTG TCCCACATAT 
CCCTATGTGC CCGCCTCGAT GCTGGAACAG TTCCATGGTC ACGGGCATGG ACATGGCCAT 
CTACCACATC CTCTGCCGCT GACTAGCACC AGCACAATGA GTCCACCAAC TACGGGGGCC 
ACCACATTGG CGCCACTACT GCTCAACACC ACGCTGGCTA CGCTTAGTAG CTCTAGCTCC 
ACCACCAGCA CCGCCAGTCC GGCACTGGCC ACCGTTATCA CAACCACAGC TCTGGCGCTC 
GCCAGTACGG TGGCCAGCGG AAGTGCCGAG GTGATTAATG CCACCATATC ATCGATCCTG 
TCCACGGTCA GCAGTTCGGT GGGCGATATC AGCGACGAGA TGACCACAGA TCCTCAGACG 
CCAGATGCAC TGCTCGAAAA TATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ent3 ( NM_140351.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648608.2 Drosophila melanogaster equilibrative nucleoside transporter 3, transcript variant A (Ent3), mRNA.

Price & Availability↑

CloneID	OFa20883
Clone ID Related Accession (Same CDS sequence)	NM_001300123.1 , NM_140351.3
Accession Version	NM_140351.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	equilibrative nucleoside transporter 3, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140351.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGTTG GTGGCCAGGA AACCACCTAT GAGCCGCTGG ACGGACGTGG TTCCGGGCGT 
TCGAGGAGCA GTCATTCCAC AACCGGATCC GGCCTGAGGC GTCCAGGCAG CATGGACAGT 
CCGGAGTTCG ATAACCGTGC ACCCAAGGAT CAGAGGCACT CCGTCTACCT GGCCCTACTG 
GCAGCGGGCA TTGGTTTTGT GTTGCCCTAC AATAGTTTCA TTATTGCGGC GGACTACTGG 
CAGGCACGAT TCCCGGGTCG CCCGGTAGCC CTGGACATGT CGATGACATA CATCTTCGTT 
GCCTTCGGCA CCGTTTTGAT GAACAACATT GTGCTATCGG TGGCACCGTT CCAGTCGCGA 
GTGCTCTTTG GCTACATGAT ATCCTTCACC ACACTGATCT TTGTCGCGGT GTGCGAGGTG 
GCTTGGCATA TGTTCGCCAC AAATACCGCC TATTTGGTGA ATATGTCAGC TGTTGCCCTG 
ACCGCCATCG GATGTACTGT GCAGCAGTCG AGCTTCTATG GATTCGCCTC CATGCTGCCC 
AAGCAGTATA CCCAGGCGGT GATGGCCGGC GAGAGTATCG CCGGTTTCCT GGTGTCTTCC 
AATCGGGTCG TTACCAAATT GCTGATTAAC AACGATCGCG TGTCCACGGT GATCTTCTTT 
TTGACCTCAA CGCTCTACAT ACTCTTCAGC TACCTGTTGC ATGTGGCCAC CATTAACTCG 
CCGTTTGTGC GGTTCCATGT GGAGGCCTGC TCCAAGATCG TTTTGCGGCC GGATGAGCAG 
GAGATTGATG GAGTTCCAAG TAGTACTCGA TATGGTGTGC TCTCGATGGA CGGTACCCAC 
ACCACGACCA CATCGGTTGG ACCTCCAGGC ACTGGAAATA CCCTTAGTTT TAGCAATCCC 
GTCTACGAGC TATCCAACCC GACTGCCGGC GAGAGTAGCA TTGAAGCCCT CGGACAGCTG 
CCCGAACTTC CGGCCACGCC CCCAGCTCAG GAGCCTACAA CGCCCACGAC GGTGGCCTTC 
AAGGTGGAAC ACGTTATCAC GCCACGTCGT TGCCGGCCCA GCAAGTTGGG TGACATCCGC 
GAGGGATTCG TGACCCGATG GCGAGTGGCC CAGGTGATCT ACCCGCACAT GGTGTGCATC 
GCCTTGGCCT ATTGTGTGAC CCTCTCGCTG TATCCGGGTA TCGAGGTTGA GGTGCAGTCA 
TGTGCCCTTC GCTCCTGGAT GCCCGTGCTG TTGATGTTCT GCTTTAATAC GTCGGATGTG 
GTTGGCAAGA TACTGGCCGC CAGTCCGTAT CCATGGTCGC GTCGCCAACT GATCCTGCTG 
TCGGGACTAA GGATTGTGCT GGTGCCGCTC CTGCTCCTCT GCTGTGCTCC CCGCCAGCGT 
CCCGTGATAT CCGGCGAGAC GGCGCCGTTT GTCTTCACCA TCGCTCTGGG CATCACGAAT 
GGATTGGCAG GCAGCCTGCC CATGATGTTG GCACCGGCAA AGGTGCCGGG AACACTCAAG 
GAGGTCACTG GCAATATAAT GACCCTGTCG TATAATGTTG GTCTGACGGT GGGCTCTCTG 
ATTGGATACG TTTTCGAGAG TATGCTGGGA CCGCAGCTGG TGAATCCGTG TCCCACATAT 
CCCTATGTGC CCGCCTCGAT GCTGGAACAG TTCCATGGTC ACGGGCATGG ACATGGCCAT 
CTACCACATC CTCTGCCGCT GACTAGCACC AGCACAATGA GTCCACCAAC TACGGGGGCC 
ACCACATTGG CGCCACTACT GCTCAACACC ACGCTGGCTA CGCTTAGTAG CTCTAGCTCC 
ACCACCAGCA CCGCCAGTCC GGCACTGGCC ACCGTTATCA CAACCACAGC TCTGGCGCTC 
GCCAGTACGG TGGCCAGCGG AAGTGCCGAG GTGATTAATG CCACCATATC ATCGATCCTG 
TCCACGGTCA GCAGTTCGGT GGGCGATATC AGCGACGAGA TGACCACAGA TCCTCAGACG 
CCAGATGCAC TGCTCGAAAA TATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648608.2
CDS	404..2410
Translation	MDVGGQETTYEPLDGRGSGRSRSSHSTTGSGLRRPGSMDSPEFDNRAPKDQRHSVYLALLAAGIGFVLPYNSFIIAADYWQARFPGRPVALDMSMTYIFVAFGTVLMNNIVLSVAPFQSRVLFGYMISFTTLIFVAVCEVAWHMFATNTAYLVNMSAVALTAIGCTVQQSSFYGFASMLPKQYTQAVMAGESIAGFLVSSNRVVTKLLINNDRVSTVIFFLTSTLYILFSYLLHVATINSPFVRFHVEACSKIVLRPDEQEIDGVPSSTRYGVLSMDGTHTTTTSVGPPGTGNTLSFSNPVYELSNPTAGESSIEALGQLPELPATPPAQEPTTPTTVAFKVEHVITPRRCRPSKLGDIREGFVTRWRVAQVIYPHMVCIALAYCVTLSLYPGIEVEVQSCALRSWMPVLLMFCFNTSDVVGKILAASPYPWSRRQLILLSGLRIVLVPLLLLCCAPRQRPVISGETAPFVFTIALGITNGLAGSLPMMLAPAKVPGTLKEVTGNIMTLSYNVGLTVGSLIGYVFESMLGPQLVNPCPTYPYVPASMLEQFHGHGHGHGHLPHPLPLTSTSTMSPPTTGATTLAPLLLNTTLATLSSSSSTTSTASPALATVITTTALALASTVASGSAEVINATISSILSTVSSSVGDISDEMTTDPQTPDALLENI

Target ORF information:

RefSeq Version	NM_140351.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster equilibrative nucleoside transporter 3, transcript variant A (Ent3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140351.3

ORF Insert Sequence:

ATGGATGTTG GTGGCCAGGA AACCACCTAT GAGCCGCTGG ACGGACGTGG TTCCGGGCGT 
TCGAGGAGCA GTCATTCCAC AACCGGATCC GGCCTGAGGC GTCCAGGCAG CATGGACAGT 
CCGGAGTTCG ATAACCGTGC ACCCAAGGAT CAGAGGCACT CCGTCTACCT GGCCCTACTG 
GCAGCGGGCA TTGGTTTTGT GTTGCCCTAC AATAGTTTCA TTATTGCGGC GGACTACTGG 
CAGGCACGAT TCCCGGGTCG CCCGGTAGCC CTGGACATGT CGATGACATA CATCTTCGTT 
GCCTTCGGCA CCGTTTTGAT GAACAACATT GTGCTATCGG TGGCACCGTT CCAGTCGCGA 
GTGCTCTTTG GCTACATGAT ATCCTTCACC ACACTGATCT TTGTCGCGGT GTGCGAGGTG 
GCTTGGCATA TGTTCGCCAC AAATACCGCC TATTTGGTGA ATATGTCAGC TGTTGCCCTG 
ACCGCCATCG GATGTACTGT GCAGCAGTCG AGCTTCTATG GATTCGCCTC CATGCTGCCC 
AAGCAGTATA CCCAGGCGGT GATGGCCGGC GAGAGTATCG CCGGTTTCCT GGTGTCTTCC 
AATCGGGTCG TTACCAAATT GCTGATTAAC AACGATCGCG TGTCCACGGT GATCTTCTTT 
TTGACCTCAA CGCTCTACAT ACTCTTCAGC TACCTGTTGC ATGTGGCCAC CATTAACTCG 
CCGTTTGTGC GGTTCCATGT GGAGGCCTGC TCCAAGATCG TTTTGCGGCC GGATGAGCAG 
GAGATTGATG GAGTTCCAAG TAGTACTCGA TATGGTGTGC TCTCGATGGA CGGTACCCAC 
ACCACGACCA CATCGGTTGG ACCTCCAGGC ACTGGAAATA CCCTTAGTTT TAGCAATCCC 
GTCTACGAGC TATCCAACCC GACTGCCGGC GAGAGTAGCA TTGAAGCCCT CGGACAGCTG 
CCCGAACTTC CGGCCACGCC CCCAGCTCAG GAGCCTACAA CGCCCACGAC GGTGGCCTTC 
AAGGTGGAAC ACGTTATCAC GCCACGTCGT TGCCGGCCCA GCAAGTTGGG TGACATCCGC 
GAGGGATTCG TGACCCGATG GCGAGTGGCC CAGGTGATCT ACCCGCACAT GGTGTGCATC 
GCCTTGGCCT ATTGTGTGAC CCTCTCGCTG TATCCGGGTA TCGAGGTTGA GGTGCAGTCA 
TGTGCCCTTC GCTCCTGGAT GCCCGTGCTG TTGATGTTCT GCTTTAATAC GTCGGATGTG 
GTTGGCAAGA TACTGGCCGC CAGTCCGTAT CCATGGTCGC GTCGCCAACT GATCCTGCTG 
TCGGGACTAA GGATTGTGCT GGTGCCGCTC CTGCTCCTCT GCTGTGCTCC CCGCCAGCGT 
CCCGTGATAT CCGGCGAGAC GGCGCCGTTT GTCTTCACCA TCGCTCTGGG CATCACGAAT 
GGATTGGCAG GCAGCCTGCC CATGATGTTG GCACCGGCAA AGGTGCCGGG AACACTCAAG 
GAGGTCACTG GCAATATAAT GACCCTGTCG TATAATGTTG GTCTGACGGT GGGCTCTCTG 
ATTGGATACG TTTTCGAGAG TATGCTGGGA CCGCAGCTGG TGAATCCGTG TCCCACATAT 
CCCTATGTGC CCGCCTCGAT GCTGGAACAG TTCCATGGTC ACGGGCATGG ACATGGCCAT 
CTACCACATC CTCTGCCGCT GACTAGCACC AGCACAATGA GTCCACCAAC TACGGGGGCC 
ACCACATTGG CGCCACTACT GCTCAACACC ACGCTGGCTA CGCTTAGTAG CTCTAGCTCC 
ACCACCAGCA CCGCCAGTCC GGCACTGGCC ACCGTTATCA CAACCACAGC TCTGGCGCTC 
GCCAGTACGG TGGCCAGCGG AAGTGCCGAG GTGATTAATG CCACCATATC ATCGATCCTG 
TCCACGGTCA GCAGTTCGGT GGGCGATATC AGCGACGAGA TGACCACAGA TCCTCAGACG 
CCAGATGCAC TGCTCGAAAA TATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ent3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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