Vps13D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vps13D
Entrez Gene ID	39448
Full Name	CG32113 gene product from transcript CG32113-RB
Synonyms	CG11002,CG11003,Dmel\CG32113
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 69E1-69E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_168512.4	NP_729825.2	vacuolar protein sorting 13D

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG32113	NP_729825.2
Canis lupus familiaris (dog)	VPS13D	XP_535400.4
Gallus gallus (chicken)	VPS13D	XP_417637.3
Caenorhabditis elegans (roundworm)	CELE_C25H3.8	NP_495110.3
Danio rerio (zebrafish)	vps13d	XP_001919988.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009780	XP_318874.4
Homo sapiens (human)	VPS13D	NP_060626.2
Bos taurus (cattle)	VPS13D	NP_001179696.2
Mus musculus (house mouse)	Vps13d	NP_001263394.1
Pan troglodytes (chimpanzee)	VPS13D	XP_001145053.1
Rattus norvegicus (Norway rat)	Vps13d	NP_001101476.1
Xenopus tropicalis (tropical clawed frog)	vps13d	XP_002937514.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Vps13D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vps13D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa20863	NM_168512.4 Latest version!	Drosophila melanogaster vacuolar protein sorting 13D (Vps13D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vps13D ( NM_168512.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729825.2 Drosophila melanogaster vacuolar protein sorting 13D (Vps13D), mRNA.

Price & Availability↑

CloneID	OFa20863
Clone ID Related Accession (Same CDS sequence)	NM_168512.4
Accession Version	NM_168512.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar protein sorting 13D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_168512.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCGAG ACCTCATAAC GTGGGTGCTG AACACGTATC TGGGAAAGTA TCTGGAGAAC 
TTGAATTCCG CCCAATTGTC GGTGGCACTG CTCTCGGGCG AAGTTGAGCT CGAGAACATA 
CCCATACGCA AAGATGCCCT CCGCTCGTTC AATTTGCCGG TCGAAGTCAC CGCCGGCAGT 
ATCCGTAAAA TCAAACTCCA GATTCCGGTC CGCCAGTTCA GGACATCGCC ATGGTGCATC 
TCCATCGAGG GCCTGTTCTG CATCATCTGT CCGAAGAACC TGGACAACTG GGACTACGAG 
AAGGAGAAGT TGCAGGATTT GGAGTATAAG CTGGCCGTGC TGGACACCGC AGAAGCAGGC 
TGGCGTTCCG AAAAGGGCAA ACAAATGGAG TCTTACTACT TCTCATCCTA CAATAACTGG 
CTGAAGTATG GCACCAACAT GGCCACCAAC ATCATTGACA ACATTGAACT CAAGATATCC 
GATGTGCACT TTCGGTTCGA GGACATCGTG GACACCGGCA AGAGCAAGAT ATGCACGGGC 
ATCAAGATTG GATCTCTGAC CGCCCAGAGC TGCGACTGTG ATTGGACCAA TGGGTCCTAC 
AAAATGAACA ACAACGAAAT GAACTACAAG CTGGTGGAGC TCAAAGAGCT CTCGGTCTAC 
TGGGATTTGT TGCACGAGGA CATCAAATGT CAGTCGTACT CTAACCAGGA GATTCTCGAA 
AAAATGCACT CCACCTGCGA ACTGAGGTCC CACAATTTCA TCATCAAGCC CATTTGTGCA 
ACGGCCAGGT GGAAGCGCGA TAAATGCCAG CAGGTGATAC GTACCAAAGA CAAACCCAGG 
GTTTCCTGTG AACTCCTGGT TCCCGAGGTG GTCATAGACA TATCTAAGGT CCAGCGACTG 
CAAATGTTAG ATAAGCTGTC AGAAATCCGT CAGGTTAAGG AAGTTCGACA ATACAGGCTA 
AAGCGACCCA CATGCACCGT AGAAAGTAAT CCAATCGCAT GGTGGAAATA CGCCACCATA 
TGCCATGGAT TTGACTTTAA GAAAAATGAG GAAAAGTGGT TAATGCTCAA GGAAAATCTT 
CGTTACATGC TGCTCTACAA ATCAATTATA TTAAATCCCA ACGAAAATCT ATCCGCTGCA 
GATAAGGAGT TTAAAGCCTA CATCGAGAGC GATAGAAAGA TATCGGATTT AACCATAATG 
AGAAGGATAT GCTTTGAAAA AGTGTTTACG AAAGGTTTCG CCTTTGAATC ACAGAACGAG 
CAGGGCAAGA ATATGCTCTT TCATTGGTTT CCAAACTGGA TGGGTTGGTA TGCGAACAGT 
CCCAGTACCC CAAATAATGA ACAGGATGAA TCGCTGAAAC ATTTGGAAGA TGATATTCTC 
GTTGCGTTGG AGAACTCACT TCAGAACTCT TCCGACTTAA AAAGCGATGC TGTCTTCGGA 
CACTTTTCAA TCAAGCTTCT CAAAGGACTG GTAATCCTGC AGACCGAAGA TAAATTAAAT 
GATGGCAGGA ACAAGTCTAT GGAAATGCAA TTCAACAACT TCTCCGCCTA CCTCCAGTTG 
AGCCCACAGT TAACTTCTTA CACTGTGGGC ATATCCTTAC AAGAAGTATA CCTAATAGAC 
AAAACCAGTA GTGATACGAT GCACAATTAC TTAATTAAGC CACAAACAGG GAATACAGCC 
ACACCAAATC AACTAGTAAA GAATGCTGCT CTCCAGGAGG ATATCCTGTT TCAATTACAA 
TACGAAAATT GCAATCATTT ACGCTTTCAG CTGAACATTA AATCAAAAGG TCTAGATCTT 
ATCTATAATG AGGATGCGAT CCAGTGGCTT TTAGATTTTC TGGCCGATTC GAATTCCTTT 
AAATACTCAC CGCGCAATAG AGTCGCGAAG AAAACTGACT TTATGAAGAA CTGGAATGAA 
ATGTTTAGTG GAAATGAGGT AAATCGAAAA ATTTGGATGT TCGAAATTGA AATTTTCGCG 
CCACGCATAA TATTTTTGGA AAACTACAAA GTTAGCAATT CGTTGATGGT TTTGCTCGAT 
TTCGGAAAAC TCGAAATGAG GAAAATGGAG GTTAAGAGAG TTATACCTGT GATAGAGTCG 
GCTGTTACTG AAAACACAAG CGATGACGAT GAAACCTATT TGACTCCATG TTCAACTCCG 
CCTGCATCGG AAAAAAGTGG TTCGGAATCT CCGACGCTGC TTGAAAATCC AAAAACGGAA 
AGCTTTCTGA ACAAAAACGT TCAACTAGAG TACGTTTTGC ACAATAAGAT TTACGATAAA 
TATTTGATCA ACTTTACAAA CCTTCAAGTT CTGGTTTGTA AATACGAAGA ACGATGGCAA 
GCGTGCTTGA AAACATCGTC GAATTTCCAT CTGATTGATA AATTTAATAT AAACCTCACA 
CTCGAGCAAA GGAATATCTT CACCGTCGAT CCGGAGTATC CCTCGTTTAT GCTCTTCGGC 
ACCTGCCCAA CAATATTAAT ACACGGCAAC GAGGAACTCA TAAACAATTG TTGCAACATT 
ATGAAACCCA TTATAAAGGC TTCAAAGGAA ATGGAAAACA TTTACAGAGG CGGTAACACG 
ATTTACGCAA GCGAGAGAAT CAAAAACCTT GCGGAAGACG ATAGGAGCCG AGTTGTCATA 
GAGTTTGTAA TGGATCAACT GGTGATCGAG ATGCAATCGA CAGAAAGGAG CATTGCCGAG 
CTTCAAATTA TTGGGGCACG AGCTGGCTTA ACAAAAGAAC CGCATGAGAC GAATATTTCC 
ATGTCGGTTC ACGGATTACT CCTCGTAGAT GCCATTCAGT CTTTCGGTCC CGATTTTGAA 
CTTTTGGTGG CTAGTCATCG TCATGTTGGA ATGGATAGTT TATCTGGCAG CCTTAAACAT 
AGCGCAATTG TTTCGCCCAC TTCGCCTGGA TCGCCAAATT TTTTTGATAG GGCTACTAGT 
CCGCACATGA TCACAAAAGC AGTTCAAAAT ATTAAAATGG GAGATAGATC CACAGAATTC 
TGCGACGATG AGGACTCCAC AGCGCTCATC TCGGTTGACA TTAAAATTGT TCCGCCCAAC 
GAAAGCAATT CAATGCAACT TCACACTACG AGCATCACAT TCAACAGTTT GGACATAATT 
GCCAATCAGG ATACTATTAT TGAAATTCTA AACTTTGCTA AGAGGACGCT TTTAGCGCAA 
AATATCTTTC CAAGCGAAAG CCCTGAATCG CCAAAGGAAG CAACTGAAGC ACCAGTTGAT 
GTGGTTGACC AAGTCGATCA TAAGAGTCAT AATGAAATAT TCTTTGATTT CTTCCGACTA 
AATATTCTGC TATTGTACAC CATCAAGAGG GACAAATTTA ATGTGGGTCG AAAAGTTGGA 
ACTGTTACGC TGACGGAAGC TAAAATCAAT GCATCCTTTC AATCGGACCT ATCTATTATT 
GGTTCCCTTG GTGGAATACA AGTTATAGAC ATCACTTCCG AAGCCTTTTG CCATCCAAGA 
ATACTTTCTG TTGGCCGAGA TCAAATTTTG AGAGCTTCGG ACACAAATAA GCAAACCGTT 
CTTAGTCAGC TTTCAAATGA AATATACTCA AATAATTACA ACGAAGAAAC GAAGAGCGAC 
GAAAGTGATG CTATAAGTTT TCAAAGTCAC TGGAGTGACA AGACTACTTG CACTTTCCAA 
ATGCGAATGG CTTCGGCTTC GTATACACAT TGTCCCAGGT TTCTGCGCGA TGTAAATGCT 
TGCATTACGT ATTTTAAAAG GAGCTTGCGA GAATTTGCCA CCTCCATTGG TAATAAAGCT 
ACCGATATGG CCAAGGAGTT TGTGCAACAG GTTAGGGCAG TCGAGCAAAT CGGACCAGTT 
TACCCACAAC GAAATCGCCA GGACAATTGG CTTGATATAA TAATTAGTAG TCCCATAATT 
ATTCTGCCCA TTTCCAACAC AAGTACGAAT GTTTTGATAG CCAATTTGGG AAAGATCAGT 
TGCTCCAATG CCGTCAAATG CGATTCGGAT GAGTTTAATG AATCCTATAC GATTGAGATT 
AAAAACACAT ACGTATACTC CTTGAACATT GATGAAGGGG AATATAGTTT CAATGTTCAC 
CCTGCCAAAA ATAAGGACGC AATTCCCATA CTGCACGATA CAGCGATTAC TTTGCAACTT 
TATGCCGGAT ATAGTGACGA TAATGATGAA GACAAACAAC TTAATAGGTT CTCAATAAAA 
GGAAGTATGG TTGAGGCTCT TAAAGTATCA CTAAACCGGA AACAATATGA GTTACTTTTG 
GAATCCATTC GGTACGCAAC TAATTTCTCC AATGAGGTTC TAAATGAATC GGACGAATTG 
GATCAAAACG GAGATATTGA ACCAACTGCG AATATTGATG CCGAATCCAT TATCAGCACA 
ACTATTCAGT TCTCAGTTCC CGTATTTCAA ATTAACCTGC AGAATGAATA TCACAATGAT 
TTGATAAATT TAACATTTAA AAACTTTAAT GTAAAACATA TTTCGAAAGG ATTTGACAAG 
GATGTGGAAG TCGTGTTAAA GTCTGTGCTA ATGGAGGATC TAAAGTCTGA CCTAACATCA 
CCGTTTAGAA ATATGGTGAC CTCTGTGGAC CTCGAACAGA AGATAAAGAA AAATGAAATG 
ACATCCTCTT CGTGTCCTGA CCTACCAAGC TATTGTAACT CACTGAAAAA CCGATCCAGC 
TCCGTTCCAT CCTGCTTCTA CAACCACATG CAGGTTAAAG TCTTTGGCGG AGACCAAAAA 
TATACATCGT CCAACAAAAA TGAGTTGGGC AAAAAGAAGG AGAGTCAAAC GTTAGTTATT 
TACAAATCAC ATACCGGACG ATCTGCGCAA AATGGTAAAC TGGAGCAAAC AAGTTCAATT 
CAGTTTAATT GCTTGAATTT GGCCATTTGC GTTGAACGGT GGTATACTAT ATTCGACTTC 
TTTGGTCTGG TTTCCGTGGA TAACGTTAAT GAAAAATACC CCGAAGAGAT GAAACTCGTT 
GAAAAACATA TTGACAAAGT TTGTAGTAAG CTGAAGGTGT CAATAAGGAG CTTTAACTTT 
ACATTGATTC GCAATGAGTC GCTCTTGTCC AGAGTAAATG TGTCGAATGC CGTTTTCATG 
ATTCTTCAGG ACCCCTACTC CAAAATCGTT GAAGGCTGCT TGGGATCTGT GTCAGTTAAT 
GATCTAACCA AATATGGAAA CATTTACAAG CAGAAGTTTC TTACTAGTGG CACGGAAGCC 
TTGAATTTTG TATATAAAAA AAAACTGGTC GATCTGGAAG CTTTAAATAC TTTGGACACC 
GACTCAACCC TGCGAATTAA CATGTCTGCA GTGCATTATA TACACACAAA ACGTTTTTCA 
ACCGAGTTAC ATGTTTTCGT TAAGGATCTG TTGCAATTGC AAACGCCTGT TATTAGAAAA 
TTAAAGAAAC ACGGCAGTGA ACAGAATATG CGCCCATCGA AAATGAAGCT AGTGATTCAA 
GCTGATTCTC CAGTTATAGT GCTTCCATCA TCCTATAACA GAAATGAAGT AATAATCGCA 
TACCTTGGCC AGTTCTCTCT GAAAAATAGT TTCCACTTTG CCAGTGACAA CAATATAATA 
AGCAAAATGA GTGCAACGCC AAGTAAAGAT GAAATCCTGG ATGTAATGCG AATTGACCTG 
GTCAATATAA ACCTATTCTC CGGCGAAAGA AGCTCCATGA AAGCTAAAGC TGATCAGGAG 
AAAGATCGAA TTATAATCGC TGATATGAAC TTCCTACGAT TGGGTCAGCC GTTCTTCAAT 
GAGTCATGCT TCCTTCACCT GCAGTTGGAG AGAAACCTTT CGGCAGATGC CCATCGCGTC 
TGTCCAGACA TCAGTGTTCA AGGAACCTTC TCAAAATTGA GTGGCATCAT CAATATTCAG 
CAATACAAAC TGATTCGAAG TTTTCTGAAC AACAATATTG GAGAACAAAC CGATGATATT 
TACATGAACT ACCACAATAA CAGTTGCACA TCCATCGAAA GACTTTCAAC CATTAACCTA 
ATGCCAAAGA ATGAAGTGTC TAAGATAGTG TCCATTCTAA TTTCGATTCG AATTTTACTG 
GAAGACGTGT CACTGCTACT GGCTTTAAAT ACTTCGCAAA GTGCTGCCAT AGAACCTTTG 
GCGTGTATCC ACTTTTTGAA ATCCACTCTG GAAATCGACT TGTTCAGCGA CGGCTCACAG 
GATATTGATT TAATATCGAG CAATATTCTA ATTGTTGACG AGAGAAACGA AAGCGACAAG 
AGCAACGAAA ACGTGTTTAA AAACATATTG GAGCCATCCA AAAAGGAGGT CCGAATCGAG 
AATTCCGTAC AGGTTGAAGT CCATTGCAGG AAAAAAGCCA CTTTTTCCAA GTACACAATA 
ATGCTCAACA ACATGAGAGT GTTTGCTCTC CTCAGTTTTC TGGATCAATT GAAGTCATAT 
TTGCAAGAAG ATTCACCGGC ACCAGTGGTA AATAATGCGG CAAATCAGAT TGCTCAAAAG 
CCACAGATCG ACACCAGTAT TTCCACGGAA TATGTTGTAA ATATTACAGA CTCGGAGATT 
ATATTCGCTG AGGAATGTAG TCGCTTGGAC TCGAACGCAA TCATTTTGAA GAGTACAACT 
GTCATTTGTT ATAAACCAAA TAGCAACATT GTGCCTCTAT CTTTGGACAT TAACCATTTG 
GAAATATTTT CCTGCACCCT GGACGCCGAA GAGGAGAGTG CTTTGTCCAT AATCGATCCC 
TTTACGCTGA TTATAGAACT TCGGAGTAAT TGCTTGAACA TACTAATTCA GAAGCACTTG 
AACATTCGGC TCTCCTATGT TGATGTTAAA TTGTTTTCGA GAATGGCGCG ACTACTGCCC 
ACACAAACAT CACGACCGAA GAACGTTATA TCAAAAGCTG ATTCGGATCT GGAGAAGGCG 
GCTCCGCTCG TGGCAATGGG CTTCGAGATA TCCGACTGTC TGTACGCCAT GCAGATAAAT 
AATTGGCGAA TCAACGACGC CGCCATTTGG CTGTCCCAGC AAAAACAGAA CACCTACCGA 
AATCCCGCCC TGGAAATGAA AACCGCTGTA GTGGATGCCA GTCTCATATC GGTGTTTATA 
ATTGACGATT GCATGGATGC AGATGTGCCA CTACTAGAGG TATCGCTCTC GAAGTTCCTG 
CTCAACTTTA CTTTCCAAAC GCAGGATCCC AATCCCAAGG AGACAAATAT TCGACACTAT 
AGCTTGGGTA ACATTGACAC AGAAGTGTCC GTCAACTATT ATAATCGTCG TCTGAGTGGA 
TGGGAACCAG TGGCAGAGAC GTGGGAGTCC AATTTGAACT GGAAGTACAC CAAGGGTCAT 
TTGGATAACA AAAAACGCTT TGAGATTGGA ATTTCCAGCA AGCAGATGCT CAAGTTGAAC 
GTGACGTCCA CGTTTATTGA ACTGTTTCAC ATGGTTCTGA AGAACTGGAC AAACGATTTC 
AATGACAATG GAGCCAAGAA CTTTAGGCAA CGTTCCCCTT TTATTCCGTT CGCGCTCCAA 
AATCTCAGCG GAACACCACT GCTTTTTAAA CCGATTTACG CTCCACTTGG AGATTTGACG 
CGTTCCGACT TACAGCAGGT GGAATTGATC AAGAACTGGT ACTCCGTGCA GCCGAACGAG 
ACGAAAACAT TTGATTTTAG CCAGAAAAGC AAACTTAGAC ACGTGCACTC TCACCAATTG 
AATCTACACC AGATTTTCGT ACAAATCCAT GGCTGGACAT TGATTGGACC CATATCGGTG 
GACAAAGTGG GCATGTTTTT CCGCACCACA AAATTGGACT CGCAGTTTTT GACGAAAAGT 
CGGATAGTCT TCGATATATC ATTGATTGGA TCTGCCCAGA AGCTCATCAA AGTCAAGTCC 
TCGTTGGGCG TGATAAACAA ACTGGATCGG AATGTGTTCC TGAAAATGAC CTTGAAAGGC 
ACGCACAGCG ATGGACTGAG TTCAATAAGT GTAATAAAGC CAAACGATGA ACTATCTGTG 
CCGCTCAAGT TCATCGATGC CTCATTATAT GTGGCACATA ATACGAGTGA AAGCGATGCC 
TACGAGGACA CTGGTTTTAG CAATGAAGAA ATCCTGTGGA AAGCCTGCGG CAAGGATGAC 
ACTCGACAGC TACTCGCTGG CTACGATACC AACAAGAGTA TCTTGTACAC TTTCGTGAAT 
ATAAGCCGGG AAATTTATCA CTGTAAGGAA CAGAATTTAC CGGGTCATAA GATTACGCTT 
CTGCCACCGC TCAAGATAAA CAATATGCTT TGCTGTGACC TTATGTTCAA GATCCACGAG 
CATGCCACCG GCAGAATAAA CTCATCGGAA AGTGTTAATA TCTACAACGT TAACATTTGC 
CAGCCCCTCA ATCTGAGCAT CACACTGGAC AACTTTCAAC TTTCGGGCCA ACTTAAGATT 
CCCGTGAGCC ACAGGGGCGT TATAGAACCG AAACTGAAAT TGATTGACAT TCAAAAACGG 
GAACTGCACG TGCGCGTGTC CATACAATCG GTGCCAGGCA AGGGAATGGA ACTATATATC 
AGTGCTCCAG TTTGGATAAT CAACAAAACA GGACTTCCCC TGATCTACAA ACAAGAAGGT 
ACCAGTCATA CGGCAGCTGG GCAATTCGAG GAGCACGAAA CTGCGCGGCA AGTGGCGCCG 
CTAATGTTCT CGTTTTCGGA TCAGGAAGGA TCTCCGGCAC TGGTACTGCG ATTGGGAAAA 
GCTTATGGCT CCAACAATAT GTGGTGCAAA AGTTTTAGTA CGCACAAGGA TTTGGCCGAC 
AGGGATTTGC GTGCGGAAAA TACAAAGGGC AGCTATGCGA TTGGAATCAG CGTCAGGAGG 
GGCAGAGGAC TCTATGCTTG CACGACCTTT GTTACGCTCT CTCCCAGATT CCATCTGCAC 
AATCGTAGTG GCTATAAGCT GGAGTTTATG CAGCTCTGCG ACATTGTTAA CTATGACCGT 
CCCGATCCGC GTAAGATTAT ATCAGCACCC GTTGATTGCA ACTTCGCTTT CCATTGGCCC 
AACTGGGATC AGGAGCAGAT TATTTGCGTA CGAATTCCGG AGATCGAGTG CTGCTGTTGG 
TCAAAGGGGA TTCCCATCAA AGATGTCCAG AGCTTGTACA TCAATGTGCG AAATGAGTGG 
GGAGAAATGT TTTTTCTTCG CTTGGAAATC ATCTCGAAGG ATGCTACTTT TATACTCTTA 
TTCACGGACG CGCGAACTTT ACCACCACCG ATTCGAATTG ACAATTGTTC GGAAGTTGTC 
ATCAATTTCT CACAGCTGAG ATCGAAGCCC GTTTGGATTA CGCCAGTTAG ACCGCAATCT 
TCGTTATCCT ATGTCATGGA TGATCCCTTG GGCCAACAGA TTTTGCTGAT GGAGGCACCT 
GGAGGAAACA TGATAGAATT CCCGATTAAC AACAAAAACA ACATTAAGAA AACACTAACG 
TACACCAACT TCATTTACAT CGCATTTCAA GGAACTTTTG AGCGTTCAAA TGAGGAGGAA 
AACACGCACA GACAGTTGGT TTTGGGCGTA CGTGGTAAAA AAGTTGTGAT AGTTGAGAAA 
AACTCGGGTG ATCGTTCACA ATTGTGGCTA ATGAACTCGA ATGGTCAGTT GGAGCATGAA 
GGATCCACCC CGCCGATTCA AACAAATGAT GCGAATGCAG TGCGACTTGT ACTCGATCTG 
GAGAAGGCTC CGAATCCCAT GGAGTTCACC AACTTGGTGG TCCGAACACC CAATAAGCAA 
AGAGTTACAA CGCAGACATG GAGATTTGAG AATGGACGGC TGATGTGCCA TGCCAATATG 
TGCGTACAGT CTCGATTTGG AGAGAGCGGC CTGAAGCCCA ACTATGAGGC AGTCGTGGGC 
AGAACGGAAA ACAGGGCAAG TTCGAGTAAA CAAATACCCA TTGGACAACA CATTGTTGCA 
CAGAAACTAA GGCCTGGATC GGGACAGCTG GAGCTTTCGA CGAAAATGGA TGGACCCATA 
TCCACGATCG AAATATGTGA TATTAAAATT AAACAAAATT CAGTATTCCT AACTCCCGAT 
TTACTATGGA TGCATGCTTC CCTGAATAAT AGGCAAATTA CGGATAAGGG AAAAGTATCA 
TTCGTGCATG AGTATTTGAT TAATGTGGAG CTTGTTAAAG GAATCGGCAT ATCCATCATA 
GCTCGCAAGC CTTGTGAGGA AATCATGTTC ATCAGCCTCG ATCACATAAA TTGTGATATT 
GTACAGAGTG CTTTGGAGAA TTCCTTAGAT CTGAATATTG CCTATATACA AATCGATAAT 
CAATTGCTGG ATGCTGTGAG TCCGATTGCA TTGCACACGC AAACTTCGAA TGATCTTGAA 
GAAACACAAA ATGCAGTTGT TCTCAAACTG AAAATGTTAC CTTCGCCCAA CAAGAATGCA 
ATCATTTTCA AGTACTTAAC TCTGGACTTG AAACCCTCAA CGGCGAGTCT GGAGGAGAAG 
CTGATACTTA AAGTGGCTTC ATTCCTGGGC TACGGCAAGA TCAACCGTCA GAACTTAAGT 
GTGCAATACC AATTCGAGAA CACGGATGAT AAGCCTTTTC TGCAGGATAT GAAGAGATAC 
TATTTTGAGA ACTTAAGTAT TGGCGCAACT CAGGTTCGAC TTTCAGCATT CACTTCTTCA 
AAGTTGCCGG TGGAGTTGCA TGAAACCAAG AAGGCACTTG GACTCACTCT AATAAAATTC 
GAAGATGCCT TGATTGAACT CGACAGGTAT TCGGATAAAT TGCACTTTGA GACGATGGAC 
GTTTATCGGA AGGAGCTGAA GAAGCACTAC ATCAACCAAG TCAAATGGCA TGCGGCTGCA 
ATTTTGGGCA GCGTCGATTT CCTTGGTAAT CCATTGGGAT TTGCCAACGA TCTTTCGGAA 
GGAGTATCCG GACTGATTTT TGAGGGCTCC GTCAAGAGTT TGGTGAAAAA TGTTACGCAT 
GGCATATCGA ATTCGACGGC GAAGCTAACA GAAACGCTGT CCGATAGCCT GGGAAAAGTT 
GTGCTGGATG ATCACGACAA TGAGACGAGG CAACGCATTC TGGAACTGCA GTCCAATACA 
TCCGGCGGTC ACCTTGCAGC GGGTCTGAAG GGTCTCGGAT TTGGACTGCT GGGCGGAGTA 
ACGAGTATTG TAAGACACAC TTACGATGGT GCCACTTCCG ATGGCGTTCC TGGCTTTCTC 
AGTGGATTGG GAAAGGGCTT AGTAGGCACT GTCACCAAAC CGATCATTGG TGTATTGGAC 
CTGGCATCGG AAACTGCCAG TGCCGTTCGT GAAACAAGCC GAGATAGTCA CAGAAACGCT 
CCGGAAAGGA AAAGGCTACC CAGATGTGTG ACTGGTGCTC CTGGTGGTCT TTTGCCATTG 
TATTCCAATC GCCAAAGCAA GGGGCAGCAA TATCTGTACC TAATCAATCA AAAGAACTTT 
TCGGAGAAGA TAATTTCATA TGAACCCAAT CTATGGAGCG ACAAGGAGGC TAGATTGCGC 
TTGCTGGTTT CTACAGAATA CGTACGAATC TTTTCACTAA GCGACGCCAA TCCAACCATT 
ATGTTTGAAT GTCATGTTAG TGAGATTCTT TCGTGCCATC CAGTGGTAAC GAATGCCGGC 
ACAACGCCAA CCACGAGCAG CAGGGCGTCG GCCAGTCACT ACATCGAGAT ATCAACCAAT 
CTTCCAAAGA TTACCAGGCC CAGGATACGG TGTCGTTCCG AGGAATGTGC TGAGGCAGCA 
TCAAGATGCA TAAACTACGC TAAAAGTGTC TTTGATGAAC GTGAACATGC CGTTTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729825.2
CDS	98..11857
Translation	MLRDLITWVLNTYLGKYLENLNSAQLSVALLSGEVELENIPIRKDALRSFNLPVEVTAGSIRKIKLQIPVRQFRTSPWCISIEGLFCIICPKNLDNWDYEKEKLQDLEYKLAVLDTAEAGWRSEKGKQMESYYFSSYNNWLKYGTNMATNIIDNIELKISDVHFRFEDIVDTGKSKICTGIKIGSLTAQSCDCDWTNGSYKMNNNEMNYKLVELKELSVYWDLLHEDIKCQSYSNQEILEKMHSTCELRSHNFIIKPICATARWKRDKCQQVIRTKDKPRVSCELLVPEVVIDISKVQRLQMLDKLSEIRQVKEVRQYRLKRPTCTVESNPIAWWKYATICHGFDFKKNEEKWLMLKENLRYMLLYKSIILNPNENLSAADKEFKAYIESDRKISDLTIMRRICFEKVFTKGFAFESQNEQGKNMLFHWFPNWMGWYANSPSTPNNEQDESLKHLEDDILVALENSLQNSSDLKSDAVFGHFSIKLLKGLVILQTEDKLNDGRNKSMEMQFNNFSAYLQLSPQLTSYTVGISLQEVYLIDKTSSDTMHNYLIKPQTGNTATPNQLVKNAALQEDILFQLQYENCNHLRFQLNIKSKGLDLIYNEDAIQWLLDFLADSNSFKYSPRNRVAKKTDFMKNWNEMFSGNEVNRKIWMFEIEIFAPRIIFLENYKVSNSLMVLLDFGKLEMRKMEVKRVIPVIESAVTENTSDDDETYLTPCSTPPASEKSGSESPTLLENPKTESFLNKNVQLEYVLHNKIYDKYLINFTNLQVLVCKYEERWQACLKTSSNFHLIDKFNINLTLEQRNIFTVDPEYPSFMLFGTCPTILIHGNEELINNCCNIMKPIIKASKEMENIYRGGNTIYASERIKNLAEDDRSRVVIEFVMDQLVIEMQSTERSIAELQIIGARAGLTKEPHETNISMSVHGLLLVDAIQSFGPDFELLVASHRHVGMDSLSGSLKHSAIVSPTSPGSPNFFDRATSPHMITKAVQNIKMGDRSTEFCDDEDSTALISVDIKIVPPNESNSMQLHTTSITFNSLDIIANQDTIIEILNFAKRTLLAQNIFPSESPESPKEATEAPVDVVDQVDHKSHNEIFFDFFRLNILLLYTIKRDKFNVGRKVGTVTLTEAKINASFQSDLSIIGSLGGIQVIDITSEAFCHPRILSVGRDQILRASDTNKQTVLSQLSNEIYSNNYNEETKSDESDAISFQSHWSDKTTCTFQMRMASASYTHCPRFLRDVNACITYFKRSLREFATSIGNKATDMAKEFVQQVRAVEQIGPVYPQRNRQDNWLDIIISSPIIILPISNTSTNVLIANLGKISCSNAVKCDSDEFNESYTIEIKNTYVYSLNIDEGEYSFNVHPAKNKDAIPILHDTAITLQLYAGYSDDNDEDKQLNRFSIKGSMVEALKVSLNRKQYELLLESIRYATNFSNEVLNESDELDQNGDIEPTANIDAESIISTTIQFSVPVFQINLQNEYHNDLINLTFKNFNVKHISKGFDKDVEVVLKSVLMEDLKSDLTSPFRNMVTSVDLEQKIKKNEMTSSSCPDLPSYCNSLKNRSSSVPSCFYNHMQVKVFGGDQKYTSSNKNELGKKKESQTLVIYKSHTGRSAQNGKLEQTSSIQFNCLNLAICVERWYTIFDFFGLVSVDNVNEKYPEEMKLVEKHIDKVCSKLKVSIRSFNFTLIRNESLLSRVNVSNAVFMILQDPYSKIVEGCLGSVSVNDLTKYGNIYKQKFLTSGTEALNFVYKKKLVDLEALNTLDTDSTLRINMSAVHYIHTKRFSTELHVFVKDLLQLQTPVIRKLKKHGSEQNMRPSKMKLVIQADSPVIVLPSSYNRNEVIIAYLGQFSLKNSFHFASDNNIISKMSATPSKDEILDVMRIDLVNINLFSGERSSMKAKADQEKDRIIIADMNFLRLGQPFFNESCFLHLQLERNLSADAHRVCPDISVQGTFSKLSGIINIQQYKLIRSFLNNNIGEQTDDIYMNYHNNSCTSIERLSTINLMPKNEVSKIVSILISIRILLEDVSLLLALNTSQSAAIEPLACIHFLKSTLEIDLFSDGSQDIDLISSNILIVDERNESDKSNENVFKNILEPSKKEVRIENSVQVEVHCRKKATFSKYTIMLNNMRVFALLSFLDQLKSYLQEDSPAPVVNNAANQIAQKPQIDTSISTEYVVNITDSEIIFAEECSRLDSNAIILKSTTVICYKPNSNIVPLSLDINHLEIFSCTLDAEEESALSIIDPFTLIIELRSNCLNILIQKHLNIRLSYVDVKLFSRMARLLPTQTSRPKNVISKADSDLEKAAPLVAMGFEISDCLYAMQINNWRINDAAIWLSQQKQNTYRNPALEMKTAVVDASLISVFIIDDCMDADVPLLEVSLSKFLLNFTFQTQDPNPKETNIRHYSLGNIDTEVSVNYYNRRLSGWEPVAETWESNLNWKYTKGHLDNKKRFEIGISSKQMLKLNVTSTFIELFHMVLKNWTNDFNDNGAKNFRQRSPFIPFALQNLSGTPLLFKPIYAPLGDLTRSDLQQVELIKNWYSVQPNETKTFDFSQKSKLRHVHSHQLNLHQIFVQIHGWTLIGPISVDKVGMFFRTTKLDSQFLTKSRIVFDISLIGSAQKLIKVKSSLGVINKLDRNVFLKMTLKGTHSDGLSSISVIKPNDELSVPLKFIDASLYVAHNTSESDAYEDTGFSNEEILWKACGKDDTRQLLAGYDTNKSILYTFVNISREIYHCKEQNLPGHKITLLPPLKINNMLCCDLMFKIHEHATGRINSSESVNIYNVNICQPLNLSITLDNFQLSGQLKIPVSHRGVIEPKLKLIDIQKRELHVRVSIQSVPGKGMELYISAPVWIINKTGLPLIYKQEGTSHTAAGQFEEHETARQVAPLMFSFSDQEGSPALVLRLGKAYGSNNMWCKSFSTHKDLADRDLRAENTKGSYAIGISVRRGRGLYACTTFVTLSPRFHLHNRSGYKLEFMQLCDIVNYDRPDPRKIISAPVDCNFAFHWPNWDQEQIICVRIPEIECCCWSKGIPIKDVQSLYINVRNEWGEMFFLRLEIISKDATFILLFTDARTLPPPIRIDNCSEVVINFSQLRSKPVWITPVRPQSSLSYVMDDPLGQQILLMEAPGGNMIEFPINNKNNIKKTLTYTNFIYIAFQGTFERSNEEENTHRQLVLGVRGKKVVIVEKNSGDRSQLWLMNSNGQLEHEGSTPPIQTNDANAVRLVLDLEKAPNPMEFTNLVVRTPNKQRVTTQTWRFENGRLMCHANMCVQSRFGESGLKPNYEAVVGRTENRASSSKQIPIGQHIVAQKLRPGSGQLELSTKMDGPISTIEICDIKIKQNSVFLTPDLLWMHASLNNRQITDKGKVSFVHEYLINVELVKGIGISIIARKPCEEIMFISLDHINCDIVQSALENSLDLNIAYIQIDNQLLDAVSPIALHTQTSNDLEETQNAVVLKLKMLPSPNKNAIIFKYLTLDLKPSTASLEEKLILKVASFLGYGKINRQNLSVQYQFENTDDKPFLQDMKRYYFENLSIGATQVRLSAFTSSKLPVELHETKKALGLTLIKFEDALIELDRYSDKLHFETMDVYRKELKKHYINQVKWHAAAILGSVDFLGNPLGFANDLSEGVSGLIFEGSVKSLVKNVTHGISNSTAKLTETLSDSLGKVVLDDHDNETRQRILELQSNTSGGHLAAGLKGLGFGLLGGVTSIVRHTYDGATSDGVPGFLSGLGKGLVGTVTKPIIGVLDLASETASAVRETSRDSHRNAPERKRLPRCVTGAPGGLLPLYSNRQSKGQQYLYLINQKNFSEKIISYEPNLWSDKEARLRLLVSTEYVRIFSLSDANPTIMFECHVSEILSCHPVVTNAGTTPTTSSRASASHYIEISTNLPKITRPRIRCRSEECAEAASRCINYAKSVFDEREHAVL

Target ORF information:

RefSeq Version	NM_168512.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar protein sorting 13D (Vps13D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168512.4

ORF Insert Sequence:

ATGTTGCGAG ACCTCATAAC GTGGGTGCTG AACACGTATC TGGGAAAGTA TCTGGAGAAC 
TTGAATTCCG CCCAATTGTC GGTGGCACTG CTCTCGGGCG AAGTTGAGCT CGAGAACATA 
CCCATACGCA AAGATGCCCT CCGCTCGTTC AATTTGCCGG TCGAAGTCAC CGCCGGCAGT 
ATCCGTAAAA TCAAACTCCA GATTCCGGTC CGCCAGTTCA GGACATCGCC ATGGTGCATC 
TCCATCGAGG GCCTGTTCTG CATCATCTGT CCGAAGAACC TGGACAACTG GGACTACGAG 
AAGGAGAAGT TGCAGGATTT GGAGTATAAG CTGGCCGTGC TGGACACCGC AGAAGCAGGC 
TGGCGTTCCG AAAAGGGCAA ACAAATGGAG TCTTACTACT TCTCATCCTA CAATAACTGG 
CTGAAGTATG GCACCAACAT GGCCACCAAC ATCATTGACA ACATTGAACT CAAGATATCC 
GATGTGCACT TTCGGTTCGA GGACATCGTG GACACCGGCA AGAGCAAGAT ATGCACGGGC 
ATCAAGATTG GATCTCTGAC CGCCCAGAGC TGCGACTGTG ATTGGACCAA TGGGTCCTAC 
AAAATGAACA ACAACGAAAT GAACTACAAG CTGGTGGAGC TCAAAGAGCT CTCGGTCTAC 
TGGGATTTGT TGCACGAGGA CATCAAATGT CAGTCGTACT CTAACCAGGA GATTCTCGAA 
AAAATGCACT CCACCTGCGA ACTGAGGTCC CACAATTTCA TCATCAAGCC CATTTGTGCA 
ACGGCCAGGT GGAAGCGCGA TAAATGCCAG CAGGTGATAC GTACCAAAGA CAAACCCAGG 
GTTTCCTGTG AACTCCTGGT TCCCGAGGTG GTCATAGACA TATCTAAGGT CCAGCGACTG 
CAAATGTTAG ATAAGCTGTC AGAAATCCGT CAGGTTAAGG AAGTTCGACA ATACAGGCTA 
AAGCGACCCA CATGCACCGT AGAAAGTAAT CCAATCGCAT GGTGGAAATA CGCCACCATA 
TGCCATGGAT TTGACTTTAA GAAAAATGAG GAAAAGTGGT TAATGCTCAA GGAAAATCTT 
CGTTACATGC TGCTCTACAA ATCAATTATA TTAAATCCCA ACGAAAATCT ATCCGCTGCA 
GATAAGGAGT TTAAAGCCTA CATCGAGAGC GATAGAAAGA TATCGGATTT AACCATAATG 
AGAAGGATAT GCTTTGAAAA AGTGTTTACG AAAGGTTTCG CCTTTGAATC ACAGAACGAG 
CAGGGCAAGA ATATGCTCTT TCATTGGTTT CCAAACTGGA TGGGTTGGTA TGCGAACAGT 
CCCAGTACCC CAAATAATGA ACAGGATGAA TCGCTGAAAC ATTTGGAAGA TGATATTCTC 
GTTGCGTTGG AGAACTCACT TCAGAACTCT TCCGACTTAA AAAGCGATGC TGTCTTCGGA 
CACTTTTCAA TCAAGCTTCT CAAAGGACTG GTAATCCTGC AGACCGAAGA TAAATTAAAT 
GATGGCAGGA ACAAGTCTAT GGAAATGCAA TTCAACAACT TCTCCGCCTA CCTCCAGTTG 
AGCCCACAGT TAACTTCTTA CACTGTGGGC ATATCCTTAC AAGAAGTATA CCTAATAGAC 
AAAACCAGTA GTGATACGAT GCACAATTAC TTAATTAAGC CACAAACAGG GAATACAGCC 
ACACCAAATC AACTAGTAAA GAATGCTGCT CTCCAGGAGG ATATCCTGTT TCAATTACAA 
TACGAAAATT GCAATCATTT ACGCTTTCAG CTGAACATTA AATCAAAAGG TCTAGATCTT 
ATCTATAATG AGGATGCGAT CCAGTGGCTT TTAGATTTTC TGGCCGATTC GAATTCCTTT 
AAATACTCAC CGCGCAATAG AGTCGCGAAG AAAACTGACT TTATGAAGAA CTGGAATGAA 
ATGTTTAGTG GAAATGAGGT AAATCGAAAA ATTTGGATGT TCGAAATTGA AATTTTCGCG 
CCACGCATAA TATTTTTGGA AAACTACAAA GTTAGCAATT CGTTGATGGT TTTGCTCGAT 
TTCGGAAAAC TCGAAATGAG GAAAATGGAG GTTAAGAGAG TTATACCTGT GATAGAGTCG 
GCTGTTACTG AAAACACAAG CGATGACGAT GAAACCTATT TGACTCCATG TTCAACTCCG 
CCTGCATCGG AAAAAAGTGG TTCGGAATCT CCGACGCTGC TTGAAAATCC AAAAACGGAA 
AGCTTTCTGA ACAAAAACGT TCAACTAGAG TACGTTTTGC ACAATAAGAT TTACGATAAA 
TATTTGATCA ACTTTACAAA CCTTCAAGTT CTGGTTTGTA AATACGAAGA ACGATGGCAA 
GCGTGCTTGA AAACATCGTC GAATTTCCAT CTGATTGATA AATTTAATAT AAACCTCACA 
CTCGAGCAAA GGAATATCTT CACCGTCGAT CCGGAGTATC CCTCGTTTAT GCTCTTCGGC 
ACCTGCCCAA CAATATTAAT ACACGGCAAC GAGGAACTCA TAAACAATTG TTGCAACATT 
ATGAAACCCA TTATAAAGGC TTCAAAGGAA ATGGAAAACA TTTACAGAGG CGGTAACACG 
ATTTACGCAA GCGAGAGAAT CAAAAACCTT GCGGAAGACG ATAGGAGCCG AGTTGTCATA 
GAGTTTGTAA TGGATCAACT GGTGATCGAG ATGCAATCGA CAGAAAGGAG CATTGCCGAG 
CTTCAAATTA TTGGGGCACG AGCTGGCTTA ACAAAAGAAC CGCATGAGAC GAATATTTCC 
ATGTCGGTTC ACGGATTACT CCTCGTAGAT GCCATTCAGT CTTTCGGTCC CGATTTTGAA 
CTTTTGGTGG CTAGTCATCG TCATGTTGGA ATGGATAGTT TATCTGGCAG CCTTAAACAT 
AGCGCAATTG TTTCGCCCAC TTCGCCTGGA TCGCCAAATT TTTTTGATAG GGCTACTAGT 
CCGCACATGA TCACAAAAGC AGTTCAAAAT ATTAAAATGG GAGATAGATC CACAGAATTC 
TGCGACGATG AGGACTCCAC AGCGCTCATC TCGGTTGACA TTAAAATTGT TCCGCCCAAC 
GAAAGCAATT CAATGCAACT TCACACTACG AGCATCACAT TCAACAGTTT GGACATAATT 
GCCAATCAGG ATACTATTAT TGAAATTCTA AACTTTGCTA AGAGGACGCT TTTAGCGCAA 
AATATCTTTC CAAGCGAAAG CCCTGAATCG CCAAAGGAAG CAACTGAAGC ACCAGTTGAT 
GTGGTTGACC AAGTCGATCA TAAGAGTCAT AATGAAATAT TCTTTGATTT CTTCCGACTA 
AATATTCTGC TATTGTACAC CATCAAGAGG GACAAATTTA ATGTGGGTCG AAAAGTTGGA 
ACTGTTACGC TGACGGAAGC TAAAATCAAT GCATCCTTTC AATCGGACCT ATCTATTATT 
GGTTCCCTTG GTGGAATACA AGTTATAGAC ATCACTTCCG AAGCCTTTTG CCATCCAAGA 
ATACTTTCTG TTGGCCGAGA TCAAATTTTG AGAGCTTCGG ACACAAATAA GCAAACCGTT 
CTTAGTCAGC TTTCAAATGA AATATACTCA AATAATTACA ACGAAGAAAC GAAGAGCGAC 
GAAAGTGATG CTATAAGTTT TCAAAGTCAC TGGAGTGACA AGACTACTTG CACTTTCCAA 
ATGCGAATGG CTTCGGCTTC GTATACACAT TGTCCCAGGT TTCTGCGCGA TGTAAATGCT 
TGCATTACGT ATTTTAAAAG GAGCTTGCGA GAATTTGCCA CCTCCATTGG TAATAAAGCT 
ACCGATATGG CCAAGGAGTT TGTGCAACAG GTTAGGGCAG TCGAGCAAAT CGGACCAGTT 
TACCCACAAC GAAATCGCCA GGACAATTGG CTTGATATAA TAATTAGTAG TCCCATAATT 
ATTCTGCCCA TTTCCAACAC AAGTACGAAT GTTTTGATAG CCAATTTGGG AAAGATCAGT 
TGCTCCAATG CCGTCAAATG CGATTCGGAT GAGTTTAATG AATCCTATAC GATTGAGATT 
AAAAACACAT ACGTATACTC CTTGAACATT GATGAAGGGG AATATAGTTT CAATGTTCAC 
CCTGCCAAAA ATAAGGACGC AATTCCCATA CTGCACGATA CAGCGATTAC TTTGCAACTT 
TATGCCGGAT ATAGTGACGA TAATGATGAA GACAAACAAC TTAATAGGTT CTCAATAAAA 
GGAAGTATGG TTGAGGCTCT TAAAGTATCA CTAAACCGGA AACAATATGA GTTACTTTTG 
GAATCCATTC GGTACGCAAC TAATTTCTCC AATGAGGTTC TAAATGAATC GGACGAATTG 
GATCAAAACG GAGATATTGA ACCAACTGCG AATATTGATG CCGAATCCAT TATCAGCACA 
ACTATTCAGT TCTCAGTTCC CGTATTTCAA ATTAACCTGC AGAATGAATA TCACAATGAT 
TTGATAAATT TAACATTTAA AAACTTTAAT GTAAAACATA TTTCGAAAGG ATTTGACAAG 
GATGTGGAAG TCGTGTTAAA GTCTGTGCTA ATGGAGGATC TAAAGTCTGA CCTAACATCA 
CCGTTTAGAA ATATGGTGAC CTCTGTGGAC CTCGAACAGA AGATAAAGAA AAATGAAATG 
ACATCCTCTT CGTGTCCTGA CCTACCAAGC TATTGTAACT CACTGAAAAA CCGATCCAGC 
TCCGTTCCAT CCTGCTTCTA CAACCACATG CAGGTTAAAG TCTTTGGCGG AGACCAAAAA 
TATACATCGT CCAACAAAAA TGAGTTGGGC AAAAAGAAGG AGAGTCAAAC GTTAGTTATT 
TACAAATCAC ATACCGGACG ATCTGCGCAA AATGGTAAAC TGGAGCAAAC AAGTTCAATT 
CAGTTTAATT GCTTGAATTT GGCCATTTGC GTTGAACGGT GGTATACTAT ATTCGACTTC 
TTTGGTCTGG TTTCCGTGGA TAACGTTAAT GAAAAATACC CCGAAGAGAT GAAACTCGTT 
GAAAAACATA TTGACAAAGT TTGTAGTAAG CTGAAGGTGT CAATAAGGAG CTTTAACTTT 
ACATTGATTC GCAATGAGTC GCTCTTGTCC AGAGTAAATG TGTCGAATGC CGTTTTCATG 
ATTCTTCAGG ACCCCTACTC CAAAATCGTT GAAGGCTGCT TGGGATCTGT GTCAGTTAAT 
GATCTAACCA AATATGGAAA CATTTACAAG CAGAAGTTTC TTACTAGTGG CACGGAAGCC 
TTGAATTTTG TATATAAAAA AAAACTGGTC GATCTGGAAG CTTTAAATAC TTTGGACACC 
GACTCAACCC TGCGAATTAA CATGTCTGCA GTGCATTATA TACACACAAA ACGTTTTTCA 
ACCGAGTTAC ATGTTTTCGT TAAGGATCTG TTGCAATTGC AAACGCCTGT TATTAGAAAA 
TTAAAGAAAC ACGGCAGTGA ACAGAATATG CGCCCATCGA AAATGAAGCT AGTGATTCAA 
GCTGATTCTC CAGTTATAGT GCTTCCATCA TCCTATAACA GAAATGAAGT AATAATCGCA 
TACCTTGGCC AGTTCTCTCT GAAAAATAGT TTCCACTTTG CCAGTGACAA CAATATAATA 
AGCAAAATGA GTGCAACGCC AAGTAAAGAT GAAATCCTGG ATGTAATGCG AATTGACCTG 
GTCAATATAA ACCTATTCTC CGGCGAAAGA AGCTCCATGA AAGCTAAAGC TGATCAGGAG 
AAAGATCGAA TTATAATCGC TGATATGAAC TTCCTACGAT TGGGTCAGCC GTTCTTCAAT 
GAGTCATGCT TCCTTCACCT GCAGTTGGAG AGAAACCTTT CGGCAGATGC CCATCGCGTC 
TGTCCAGACA TCAGTGTTCA AGGAACCTTC TCAAAATTGA GTGGCATCAT CAATATTCAG 
CAATACAAAC TGATTCGAAG TTTTCTGAAC AACAATATTG GAGAACAAAC CGATGATATT 
TACATGAACT ACCACAATAA CAGTTGCACA TCCATCGAAA GACTTTCAAC CATTAACCTA 
ATGCCAAAGA ATGAAGTGTC TAAGATAGTG TCCATTCTAA TTTCGATTCG AATTTTACTG 
GAAGACGTGT CACTGCTACT GGCTTTAAAT ACTTCGCAAA GTGCTGCCAT AGAACCTTTG 
GCGTGTATCC ACTTTTTGAA ATCCACTCTG GAAATCGACT TGTTCAGCGA CGGCTCACAG 
GATATTGATT TAATATCGAG CAATATTCTA ATTGTTGACG AGAGAAACGA AAGCGACAAG 
AGCAACGAAA ACGTGTTTAA AAACATATTG GAGCCATCCA AAAAGGAGGT CCGAATCGAG 
AATTCCGTAC AGGTTGAAGT CCATTGCAGG AAAAAAGCCA CTTTTTCCAA GTACACAATA 
ATGCTCAACA ACATGAGAGT GTTTGCTCTC CTCAGTTTTC TGGATCAATT GAAGTCATAT 
TTGCAAGAAG ATTCACCGGC ACCAGTGGTA AATAATGCGG CAAATCAGAT TGCTCAAAAG 
CCACAGATCG ACACCAGTAT TTCCACGGAA TATGTTGTAA ATATTACAGA CTCGGAGATT 
ATATTCGCTG AGGAATGTAG TCGCTTGGAC TCGAACGCAA TCATTTTGAA GAGTACAACT 
GTCATTTGTT ATAAACCAAA TAGCAACATT GTGCCTCTAT CTTTGGACAT TAACCATTTG 
GAAATATTTT CCTGCACCCT GGACGCCGAA GAGGAGAGTG CTTTGTCCAT AATCGATCCC 
TTTACGCTGA TTATAGAACT TCGGAGTAAT TGCTTGAACA TACTAATTCA GAAGCACTTG 
AACATTCGGC TCTCCTATGT TGATGTTAAA TTGTTTTCGA GAATGGCGCG ACTACTGCCC 
ACACAAACAT CACGACCGAA GAACGTTATA TCAAAAGCTG ATTCGGATCT GGAGAAGGCG 
GCTCCGCTCG TGGCAATGGG CTTCGAGATA TCCGACTGTC TGTACGCCAT GCAGATAAAT 
AATTGGCGAA TCAACGACGC CGCCATTTGG CTGTCCCAGC AAAAACAGAA CACCTACCGA 
AATCCCGCCC TGGAAATGAA AACCGCTGTA GTGGATGCCA GTCTCATATC GGTGTTTATA 
ATTGACGATT GCATGGATGC AGATGTGCCA CTACTAGAGG TATCGCTCTC GAAGTTCCTG 
CTCAACTTTA CTTTCCAAAC GCAGGATCCC AATCCCAAGG AGACAAATAT TCGACACTAT 
AGCTTGGGTA ACATTGACAC AGAAGTGTCC GTCAACTATT ATAATCGTCG TCTGAGTGGA 
TGGGAACCAG TGGCAGAGAC GTGGGAGTCC AATTTGAACT GGAAGTACAC CAAGGGTCAT 
TTGGATAACA AAAAACGCTT TGAGATTGGA ATTTCCAGCA AGCAGATGCT CAAGTTGAAC 
GTGACGTCCA CGTTTATTGA ACTGTTTCAC ATGGTTCTGA AGAACTGGAC AAACGATTTC 
AATGACAATG GAGCCAAGAA CTTTAGGCAA CGTTCCCCTT TTATTCCGTT CGCGCTCCAA 
AATCTCAGCG GAACACCACT GCTTTTTAAA CCGATTTACG CTCCACTTGG AGATTTGACG 
CGTTCCGACT TACAGCAGGT GGAATTGATC AAGAACTGGT ACTCCGTGCA GCCGAACGAG 
ACGAAAACAT TTGATTTTAG CCAGAAAAGC AAACTTAGAC ACGTGCACTC TCACCAATTG 
AATCTACACC AGATTTTCGT ACAAATCCAT GGCTGGACAT TGATTGGACC CATATCGGTG 
GACAAAGTGG GCATGTTTTT CCGCACCACA AAATTGGACT CGCAGTTTTT GACGAAAAGT 
CGGATAGTCT TCGATATATC ATTGATTGGA TCTGCCCAGA AGCTCATCAA AGTCAAGTCC 
TCGTTGGGCG TGATAAACAA ACTGGATCGG AATGTGTTCC TGAAAATGAC CTTGAAAGGC 
ACGCACAGCG ATGGACTGAG TTCAATAAGT GTAATAAAGC CAAACGATGA ACTATCTGTG 
CCGCTCAAGT TCATCGATGC CTCATTATAT GTGGCACATA ATACGAGTGA AAGCGATGCC 
TACGAGGACA CTGGTTTTAG CAATGAAGAA ATCCTGTGGA AAGCCTGCGG CAAGGATGAC 
ACTCGACAGC TACTCGCTGG CTACGATACC AACAAGAGTA TCTTGTACAC TTTCGTGAAT 
ATAAGCCGGG AAATTTATCA CTGTAAGGAA CAGAATTTAC CGGGTCATAA GATTACGCTT 
CTGCCACCGC TCAAGATAAA CAATATGCTT TGCTGTGACC TTATGTTCAA GATCCACGAG 
CATGCCACCG GCAGAATAAA CTCATCGGAA AGTGTTAATA TCTACAACGT TAACATTTGC 
CAGCCCCTCA ATCTGAGCAT CACACTGGAC AACTTTCAAC TTTCGGGCCA ACTTAAGATT 
CCCGTGAGCC ACAGGGGCGT TATAGAACCG AAACTGAAAT TGATTGACAT TCAAAAACGG 
GAACTGCACG TGCGCGTGTC CATACAATCG GTGCCAGGCA AGGGAATGGA ACTATATATC 
AGTGCTCCAG TTTGGATAAT CAACAAAACA GGACTTCCCC TGATCTACAA ACAAGAAGGT 
ACCAGTCATA CGGCAGCTGG GCAATTCGAG GAGCACGAAA CTGCGCGGCA AGTGGCGCCG 
CTAATGTTCT CGTTTTCGGA TCAGGAAGGA TCTCCGGCAC TGGTACTGCG ATTGGGAAAA 
GCTTATGGCT CCAACAATAT GTGGTGCAAA AGTTTTAGTA CGCACAAGGA TTTGGCCGAC 
AGGGATTTGC GTGCGGAAAA TACAAAGGGC AGCTATGCGA TTGGAATCAG CGTCAGGAGG 
GGCAGAGGAC TCTATGCTTG CACGACCTTT GTTACGCTCT CTCCCAGATT CCATCTGCAC 
AATCGTAGTG GCTATAAGCT GGAGTTTATG CAGCTCTGCG ACATTGTTAA CTATGACCGT 
CCCGATCCGC GTAAGATTAT ATCAGCACCC GTTGATTGCA ACTTCGCTTT CCATTGGCCC 
AACTGGGATC AGGAGCAGAT TATTTGCGTA CGAATTCCGG AGATCGAGTG CTGCTGTTGG 
TCAAAGGGGA TTCCCATCAA AGATGTCCAG AGCTTGTACA TCAATGTGCG AAATGAGTGG 
GGAGAAATGT TTTTTCTTCG CTTGGAAATC ATCTCGAAGG ATGCTACTTT TATACTCTTA 
TTCACGGACG CGCGAACTTT ACCACCACCG ATTCGAATTG ACAATTGTTC GGAAGTTGTC 
ATCAATTTCT CACAGCTGAG ATCGAAGCCC GTTTGGATTA CGCCAGTTAG ACCGCAATCT 
TCGTTATCCT ATGTCATGGA TGATCCCTTG GGCCAACAGA TTTTGCTGAT GGAGGCACCT 
GGAGGAAACA TGATAGAATT CCCGATTAAC AACAAAAACA ACATTAAGAA AACACTAACG 
TACACCAACT TCATTTACAT CGCATTTCAA GGAACTTTTG AGCGTTCAAA TGAGGAGGAA 
AACACGCACA GACAGTTGGT TTTGGGCGTA CGTGGTAAAA AAGTTGTGAT AGTTGAGAAA 
AACTCGGGTG ATCGTTCACA ATTGTGGCTA ATGAACTCGA ATGGTCAGTT GGAGCATGAA 
GGATCCACCC CGCCGATTCA AACAAATGAT GCGAATGCAG TGCGACTTGT ACTCGATCTG 
GAGAAGGCTC CGAATCCCAT GGAGTTCACC AACTTGGTGG TCCGAACACC CAATAAGCAA 
AGAGTTACAA CGCAGACATG GAGATTTGAG AATGGACGGC TGATGTGCCA TGCCAATATG 
TGCGTACAGT CTCGATTTGG AGAGAGCGGC CTGAAGCCCA ACTATGAGGC AGTCGTGGGC 
AGAACGGAAA ACAGGGCAAG TTCGAGTAAA CAAATACCCA TTGGACAACA CATTGTTGCA 
CAGAAACTAA GGCCTGGATC GGGACAGCTG GAGCTTTCGA CGAAAATGGA TGGACCCATA 
TCCACGATCG AAATATGTGA TATTAAAATT AAACAAAATT CAGTATTCCT AACTCCCGAT 
TTACTATGGA TGCATGCTTC CCTGAATAAT AGGCAAATTA CGGATAAGGG AAAAGTATCA 
TTCGTGCATG AGTATTTGAT TAATGTGGAG CTTGTTAAAG GAATCGGCAT ATCCATCATA 
GCTCGCAAGC CTTGTGAGGA AATCATGTTC ATCAGCCTCG ATCACATAAA TTGTGATATT 
GTACAGAGTG CTTTGGAGAA TTCCTTAGAT CTGAATATTG CCTATATACA AATCGATAAT 
CAATTGCTGG ATGCTGTGAG TCCGATTGCA TTGCACACGC AAACTTCGAA TGATCTTGAA 
GAAACACAAA ATGCAGTTGT TCTCAAACTG AAAATGTTAC CTTCGCCCAA CAAGAATGCA 
ATCATTTTCA AGTACTTAAC TCTGGACTTG AAACCCTCAA CGGCGAGTCT GGAGGAGAAG 
CTGATACTTA AAGTGGCTTC ATTCCTGGGC TACGGCAAGA TCAACCGTCA GAACTTAAGT 
GTGCAATACC AATTCGAGAA CACGGATGAT AAGCCTTTTC TGCAGGATAT GAAGAGATAC 
TATTTTGAGA ACTTAAGTAT TGGCGCAACT CAGGTTCGAC TTTCAGCATT CACTTCTTCA 
AAGTTGCCGG TGGAGTTGCA TGAAACCAAG AAGGCACTTG GACTCACTCT AATAAAATTC 
GAAGATGCCT TGATTGAACT CGACAGGTAT TCGGATAAAT TGCACTTTGA GACGATGGAC 
GTTTATCGGA AGGAGCTGAA GAAGCACTAC ATCAACCAAG TCAAATGGCA TGCGGCTGCA 
ATTTTGGGCA GCGTCGATTT CCTTGGTAAT CCATTGGGAT TTGCCAACGA TCTTTCGGAA 
GGAGTATCCG GACTGATTTT TGAGGGCTCC GTCAAGAGTT TGGTGAAAAA TGTTACGCAT 
GGCATATCGA ATTCGACGGC GAAGCTAACA GAAACGCTGT CCGATAGCCT GGGAAAAGTT 
GTGCTGGATG ATCACGACAA TGAGACGAGG CAACGCATTC TGGAACTGCA GTCCAATACA 
TCCGGCGGTC ACCTTGCAGC GGGTCTGAAG GGTCTCGGAT TTGGACTGCT GGGCGGAGTA 
ACGAGTATTG TAAGACACAC TTACGATGGT GCCACTTCCG ATGGCGTTCC TGGCTTTCTC 
AGTGGATTGG GAAAGGGCTT AGTAGGCACT GTCACCAAAC CGATCATTGG TGTATTGGAC 
CTGGCATCGG AAACTGCCAG TGCCGTTCGT GAAACAAGCC GAGATAGTCA CAGAAACGCT 
CCGGAAAGGA AAAGGCTACC CAGATGTGTG ACTGGTGCTC CTGGTGGTCT TTTGCCATTG 
TATTCCAATC GCCAAAGCAA GGGGCAGCAA TATCTGTACC TAATCAATCA AAAGAACTTT 
TCGGAGAAGA TAATTTCATA TGAACCCAAT CTATGGAGCG ACAAGGAGGC TAGATTGCGC 
TTGCTGGTTT CTACAGAATA CGTACGAATC TTTTCACTAA GCGACGCCAA TCCAACCATT 
ATGTTTGAAT GTCATGTTAG TGAGATTCTT TCGTGCCATC CAGTGGTAAC GAATGCCGGC 
ACAACGCCAA CCACGAGCAG CAGGGCGTCG GCCAGTCACT ACATCGAGAT ATCAACCAAT 
CTTCCAAAGA TTACCAGGCC CAGGATACGG TGTCGTTCCG AGGAATGTGC TGAGGCAGCA 
TCAAGATGCA TAAACTACGC TAAAAGTGTC TTTGATGAAC GTGAACATGC CGTTTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vps13D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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