Klc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Klc
Entrez Gene ID	39445
Full Name	Kinesin light chain
Synonyms	CG5433,DKLC,Dmel\CG5433,KLC,kinesin,klc
General protein information	Preferred Names Kinesin light chain Names CG5433 gene product from transcript CG5433-RA CG5433-PA CG5433-PB CG5433-PC Klc-PA Klc-PB Klc-PC kinesin kinesin 1 light chain kinesin I kinesin light chain kinesin light chain subunit of kinesin-1 kinesin-1 kinesin-1 light chain
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 69E1-69E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_079325.3	NP_524049.1	kinesin light chain, isoform A
NM_001274852.1	NP_001261781.1	kinesin light chain, isoform C
NM_001274851.1	NP_001261780.1	kinesin light chain, isoform B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	KLC1	XP_005623905.1
Bos taurus (cattle)	KLC1	NP_001092385.1
Drosophila melanogaster (fruit fly)	Klc	NP_524049.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010258	XP_319447.2
Caenorhabditis elegans (roundworm)	klc-2	NP_001023664.1
Xenopus tropicalis (tropical clawed frog)	klc1	NP_001017247.1
Danio rerio (zebrafish)	klc1a	NP_957247.1
Homo sapiens (human)	KLC1	NP_001123579.1
Pan troglodytes (chimpanzee)	KLC1	XP_001139827.1
Mus musculus (house mouse)	Klc1	NP_001020531.2
Rattus norvegicus (Norway rat)	Klc1	NP_001075443.1
Gallus gallus (chicken)	KLC1	XP_421389.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that kinesin-1 spends half of each stepping cycle with one head bound, specifying a structural state for each of two rate-limiting transitions.
Title: Kinetics of nucleotide-dependent structural transitions in the kinesin-1 hydrolysis cycle.

ensconsin promotes productive recruitment of Kinesin-1 to microtubules
Title: Drosophila ensconsin promotes productive recruitment of Kinesin-1 to microtubules.

A functional consequence of myosin V's diffusion on microtubules is a significant enhancement of the processive run length of kinesin when both motors are present on the same cargo.
Title: Myosin V and Kinesin act as tethers to enhance each others' processivity.

Data show that kinesin heavy chain can function independently of the light chain in the oocyte, indicating that it associates with its cargoes by a novel mechanism.
Title: Kinesin light chain-independent function of the Kinesin heavy chain in cytoplasmic streaming and posterior localisation in the Drosophila oocyte.

The following Klc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Klc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18372	NM_001274852.1 Latest version!	Drosophila melanogaster kinesin light chain, transcript variant C (Klc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa18373	NM_079325.3 Latest version!	Drosophila melanogaster kinesin light chain, transcript variant A (Klc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa18373	NM_001274851.1 Latest version!	Drosophila melanogaster kinesin light chain, transcript variant B (Klc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Klc ( NM_001274852.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261781.1 Drosophila melanogaster kinesin light chain, transcript variant C (Klc), mRNA.

Price & Availability↑

CloneID	OFa18372
Clone ID Related Accession (Same CDS sequence)	NM_001274852.1
Accession Version	NM_001274852.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kinesin light chain, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCAAA TGTCGCAGGA CGAAATCATA ACCAATACGA AAACAGTCCT TCAAGGCCTC 
GAAGCCTTGC GAGTGGAGCA TGTTTCGATT ATGAACGGAA TCGCCGAGGT GCAGAAGGAC 
AATGAAAAGT CGGACATGCT GCGAAAGAAC ATCGAGAACA TCGAACTGGG CCTCAGCGAG 
GCCCAGGTGA TGATGGCCCT GACATCCCAT CTGCAGAACA TCGAGGCCGA GAAGCACAAG 
CTTAAGACAC AGGTGCGCCG CCTCCACCAG GAGAACGCCT GGCTTCGCGA CGAGCTGGCC 
AACACTCAGC AAAAGTTCCA GGCATCCGAG CAGCTGGTGG CCCAACTAGA GGAGGAGAAG 
AAGCACCTGG AGTTCATGGC GTCCGTGAAG AAGTACGACG AGAATCAGGA GCAGGACGAC 
GCTTGCGACA AGTCCCGCAC CGATCCCGTG GTCGAGCTAT TCCCGGACGA GGAAAACGAG 
GACAGACACA ACATGTCGCC CACTCCGCCC AGCCAGTTCG CCAACCAGAC TTCCGGCTAC 
GAGATTCCAG CGCGTCTCCG TACTCTGCAC AACCTGGTCA TTCAGTATGC ATCGCAGGGA 
AGATACGAGG TAGCCGTTCC CCTCTGCAAG CAAGCATTGG AAGATCTCGA AAGGACAAGT 
GGTCACGACC ATCCCGATGT AGCAACCATG CTGAACATTT TGGCTCTCGT GTACCGCGAT 
CAGAACAAGT ATAAGGAGGC CGCCAATTTA CTCAACGACG CTCTGTCGAT TCGAGGAAAG 
ACACTGGGCG AGAACCATCC AGCCGTGGCG GCCACGTTGA ACAACTTGGC CGTCCTCTAT 
GGCAAACGTG GCAAGTACAA AGATGCCGAG CCACTGTGCA AGCGTGCCTT GGAGATCCGA 
GAGAAAGTCC TGGGAAAGGA TCATCCCGAT GTTGCCAAGC AACTTAACAA TCTCGCCCTG 
CTCTGCCAGA ATCAGGGCAA ATACGACGAG GTTGAGAAGT ACTACCAGCG AGCTCTCGAC 
ATCTACGAAT CAAAGTTGGG TCCCGATGAT CCCAACGTGG CCAAGACAAA GAATAATCTG 
GCCGGCTGCT ATCTGAAGCA AGGTAGATAC ACCGAAGCTG AAATCCTCTA TAAGCAGGTC 
TTGACGCGAG CCCACGAACG TGAGTTTGGA GCCATCGACA GCAAAAACAA GCCCATTTGG 
CAGGTGGCTG AGGAGCGTGA GGAGCACAAA TTCGATAACA GGGAGAACAC TCCATATGGC 
GAGTACGGCG GTTGGCATAA GGCCGCTAAA GTGGATTCGC CCACGGTCAC AACCACTCTA 
AAAAATCTGG GAGCACTTTA CCGACGTCAA GGCATGTTTG AAGCGGCCGA AACCCTGGAA 
GACTGTGCAA TGCGGAGTAA AAAAGAAGCC TACGATCTAG CTAAACAAAC GAAGCTCTCA 
CAATTGCTAA CGTCAAACGA AAAGCGACGA TCGAAGGCAA TTAAAGAGGA TTTGGATTTC 
TCTGAGGAGA ATGCGAAACC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261781.1
CDS	156..1679
Translation	MTQMSQDEIITNTKTVLQGLEALRVEHVSIMNGIAEVQKDNEKSDMLRKNIENIELGLSEAQVMMALTSHLQNIEAEKHKLKTQVRRLHQENAWLRDELANTQQKFQASEQLVAQLEEEKKHLEFMASVKKYDENQEQDDACDKSRTDPVVELFPDEENEDRHNMSPTPPSQFANQTSGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLERTSGHDHPDVATMLNILALVYRDQNKYKEAANLLNDALSIRGKTLGENHPAVAATLNNLAVLYGKRGKYKDAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYDEVEKYYQRALDIYESKLGPDDPNVAKTKNNLAGCYLKQGRYTEAEILYKQVLTRAHEREFGAIDSKNKPIWQVAEEREEHKFDNRENTPYGEYGGWHKAAKVDSPTVTTTLKNLGALYRRQGMFEAAETLEDCAMRSKKEAYDLAKQTKLSQLLTSNEKRRSKAIKEDLDFSEENAKP

Target ORF information:

RefSeq Version	NM_001274852.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kinesin light chain, transcript variant C (Klc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274852.1

ORF Insert Sequence:

ATGACGCAAA TGTCGCAGGA CGAAATCATA ACCAATACGA AAACAGTCCT TCAAGGCCTC 
GAAGCCTTGC GAGTGGAGCA TGTTTCGATT ATGAACGGAA TCGCCGAGGT GCAGAAGGAC 
AATGAAAAGT CGGACATGCT GCGAAAGAAC ATCGAGAACA TCGAACTGGG CCTCAGCGAG 
GCCCAGGTGA TGATGGCCCT GACATCCCAT CTGCAGAACA TCGAGGCCGA GAAGCACAAG 
CTTAAGACAC AGGTGCGCCG CCTCCACCAG GAGAACGCCT GGCTTCGCGA CGAGCTGGCC 
AACACTCAGC AAAAGTTCCA GGCATCCGAG CAGCTGGTGG CCCAACTAGA GGAGGAGAAG 
AAGCACCTGG AGTTCATGGC GTCCGTGAAG AAGTACGACG AGAATCAGGA GCAGGACGAC 
GCTTGCGACA AGTCCCGCAC CGATCCCGTG GTCGAGCTAT TCCCGGACGA GGAAAACGAG 
GACAGACACA ACATGTCGCC CACTCCGCCC AGCCAGTTCG CCAACCAGAC TTCCGGCTAC 
GAGATTCCAG CGCGTCTCCG TACTCTGCAC AACCTGGTCA TTCAGTATGC ATCGCAGGGA 
AGATACGAGG TAGCCGTTCC CCTCTGCAAG CAAGCATTGG AAGATCTCGA AAGGACAAGT 
GGTCACGACC ATCCCGATGT AGCAACCATG CTGAACATTT TGGCTCTCGT GTACCGCGAT 
CAGAACAAGT ATAAGGAGGC CGCCAATTTA CTCAACGACG CTCTGTCGAT TCGAGGAAAG 
ACACTGGGCG AGAACCATCC AGCCGTGGCG GCCACGTTGA ACAACTTGGC CGTCCTCTAT 
GGCAAACGTG GCAAGTACAA AGATGCCGAG CCACTGTGCA AGCGTGCCTT GGAGATCCGA 
GAGAAAGTCC TGGGAAAGGA TCATCCCGAT GTTGCCAAGC AACTTAACAA TCTCGCCCTG 
CTCTGCCAGA ATCAGGGCAA ATACGACGAG GTTGAGAAGT ACTACCAGCG AGCTCTCGAC 
ATCTACGAAT CAAAGTTGGG TCCCGATGAT CCCAACGTGG CCAAGACAAA GAATAATCTG 
GCCGGCTGCT ATCTGAAGCA AGGTAGATAC ACCGAAGCTG AAATCCTCTA TAAGCAGGTC 
TTGACGCGAG CCCACGAACG TGAGTTTGGA GCCATCGACA GCAAAAACAA GCCCATTTGG 
CAGGTGGCTG AGGAGCGTGA GGAGCACAAA TTCGATAACA GGGAGAACAC TCCATATGGC 
GAGTACGGCG GTTGGCATAA GGCCGCTAAA GTGGATTCGC CCACGGTCAC AACCACTCTA 
AAAAATCTGG GAGCACTTTA CCGACGTCAA GGCATGTTTG AAGCGGCCGA AACCCTGGAA 
GACTGTGCAA TGCGGAGTAA AAAAGAAGCC TACGATCTAG CTAAACAAAC GAAGCTCTCA 
CAATTGCTAA CGTCAAACGA AAAGCGACGA TCGAAGGCAA TTAAAGAGGA TTTGGATTTC 
TCTGAGGAGA ATGCGAAACC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Klc ( NM_079325.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524049.1 Drosophila melanogaster kinesin light chain, transcript variant A (Klc), mRNA.

Price & Availability↑

CloneID	OFa18373
Clone ID Related Accession (Same CDS sequence)	NM_079325.3 , NM_001274851.1
Accession Version	NM_079325.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kinesin light chain, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Dec 17, 2009 this sequence version replaced NM_079325.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCAAA TGTCGCAGGA CGAAATCATA ACCAATACGA AAACAGTCCT TCAAGGCCTC 
GAAGCCTTGC GAGTGGAGCA TGTTTCGATT ATGAACGGAA TCGCCGAGGT GCAGAAGGAC 
AATGAAAAGT CGGACATGCT GCGAAAGAAC ATCGAGAACA TCGAACTGGG CCTCAGCGAG 
GCCCAGGTGA TGATGGCCCT GACATCCCAT CTGCAGAACA TCGAGGCCGA GAAGCACAAG 
CTTAAGACAC AGGTGCGCCG CCTCCACCAG GAGAACGCCT GGCTTCGCGA CGAGCTGGCC 
AACACTCAGC AAAAGTTCCA GGCATCCGAG CAGCTGGTGG CCCAACTAGA GGAGGAGAAG 
AAGCACCTGG AGTTCATGGC GTCCGTGAAG AAGTACGACG AGAATCAGGA GCAGGACGAC 
GCTTGCGACA AGTCCCGCAC CGATCCCGTG GTCGAGCTAT TCCCGGACGA GGAAAACGAG 
GACAGACACA ACATGTCGCC CACTCCGCCC AGCCAGTTCG CCAACCAGAC TTCCGGCTAC 
GAGATTCCAG CGCGTCTCCG TACTCTGCAC AACCTGGTCA TTCAGTATGC ATCGCAGGGA 
AGATACGAGG TAGCCGTTCC CCTCTGCAAG CAAGCATTGG AAGATCTCGA AAGGACAAGT 
GGTCACGACC ATCCCGATGT AGCAACCATG CTGAACATTT TGGCTCTCGT GTACCGCGAT 
CAGAACAAGT ATAAGGAGGC CGCCAATTTA CTCAACGACG CTCTGTCGAT TCGAGGAAAG 
ACACTGGGCG AGAACCATCC AGCCGTGGCG GCCACGTTGA ACAACTTGGC CGTCCTCTAT 
GGCAAACGTG GCAAGTACAA AGATGCCGAG CCACTGTGCA AGCGTGCCTT GGAGATCCGA 
GAGAAAGTCC TGGGAAAGGA TCATCCCGAT GTTGCCAAGC AACTTAACAA TCTCGCCCTG 
CTCTGCCAGA ATCAGGGCAA ATACGACGAG GTTGAGAAGT ACTACCAGCG AGCTCTCGAC 
ATCTACGAAT CAAAGTTGGG TCCCGATGAT CCCAACGTGG CCAAGACAAA GAATAATCTG 
GCCGGCTGCT ATCTGAAGCA AGGTAGATAC ACCGAAGCTG AAATCCTCTA TAAGCAGGTC 
TTGACGCGAG CCCACGAACG TGAGTTTGGA GCCATCGACA GCAAAAACAA GCCCATTTGG 
CAGGTGGCTG AGGAGCGTGA GGAGCACAAA TTCGATAACA GGGAGAACAC TCCATATGGC 
GAGTACGGCG GTTGGCATAA GGCCGCTAAA GTGGATTCGC CCACGGTCAC AACCACTCTA 
AAAAATCTGG GAGCACTTTA CCGACGTCAA GGCATGTTTG AAGCGGCCGA AACCCTGGAA 
GACTGTGCAA TGCGGAGTAA AAAAGAAGCC TACGATCTAG CTAAACAAAC GAAGCTCTCA 
CAATTGCTAA CGTCAAACGA AAAGCGACGA TCGAAGGCAA TTAAAGAGGA TTTGGATTTC 
TCTGAGGAGA AGAATGCGAA ACCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524049.1
CDS	120..1646
Translation	MTQMSQDEIITNTKTVLQGLEALRVEHVSIMNGIAEVQKDNEKSDMLRKNIENIELGLSEAQVMMALTSHLQNIEAEKHKLKTQVRRLHQENAWLRDELANTQQKFQASEQLVAQLEEEKKHLEFMASVKKYDENQEQDDACDKSRTDPVVELFPDEENEDRHNMSPTPPSQFANQTSGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLERTSGHDHPDVATMLNILALVYRDQNKYKEAANLLNDALSIRGKTLGENHPAVAATLNNLAVLYGKRGKYKDAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYDEVEKYYQRALDIYESKLGPDDPNVAKTKNNLAGCYLKQGRYTEAEILYKQVLTRAHEREFGAIDSKNKPIWQVAEEREEHKFDNRENTPYGEYGGWHKAAKVDSPTVTTTLKNLGALYRRQGMFEAAETLEDCAMRSKKEAYDLAKQTKLSQLLTSNEKRRSKAIKEDLDFSEEKNAKP

Target ORF information:

RefSeq Version	NM_079325.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kinesin light chain, transcript variant A (Klc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079325.3

ORF Insert Sequence:

ATGACGCAAA TGTCGCAGGA CGAAATCATA ACCAATACGA AAACAGTCCT TCAAGGCCTC 
GAAGCCTTGC GAGTGGAGCA TGTTTCGATT ATGAACGGAA TCGCCGAGGT GCAGAAGGAC 
AATGAAAAGT CGGACATGCT GCGAAAGAAC ATCGAGAACA TCGAACTGGG CCTCAGCGAG 
GCCCAGGTGA TGATGGCCCT GACATCCCAT CTGCAGAACA TCGAGGCCGA GAAGCACAAG 
CTTAAGACAC AGGTGCGCCG CCTCCACCAG GAGAACGCCT GGCTTCGCGA CGAGCTGGCC 
AACACTCAGC AAAAGTTCCA GGCATCCGAG CAGCTGGTGG CCCAACTAGA GGAGGAGAAG 
AAGCACCTGG AGTTCATGGC GTCCGTGAAG AAGTACGACG AGAATCAGGA GCAGGACGAC 
GCTTGCGACA AGTCCCGCAC CGATCCCGTG GTCGAGCTAT TCCCGGACGA GGAAAACGAG 
GACAGACACA ACATGTCGCC CACTCCGCCC AGCCAGTTCG CCAACCAGAC TTCCGGCTAC 
GAGATTCCAG CGCGTCTCCG TACTCTGCAC AACCTGGTCA TTCAGTATGC ATCGCAGGGA 
AGATACGAGG TAGCCGTTCC CCTCTGCAAG CAAGCATTGG AAGATCTCGA AAGGACAAGT 
GGTCACGACC ATCCCGATGT AGCAACCATG CTGAACATTT TGGCTCTCGT GTACCGCGAT 
CAGAACAAGT ATAAGGAGGC CGCCAATTTA CTCAACGACG CTCTGTCGAT TCGAGGAAAG 
ACACTGGGCG AGAACCATCC AGCCGTGGCG GCCACGTTGA ACAACTTGGC CGTCCTCTAT 
GGCAAACGTG GCAAGTACAA AGATGCCGAG CCACTGTGCA AGCGTGCCTT GGAGATCCGA 
GAGAAAGTCC TGGGAAAGGA TCATCCCGAT GTTGCCAAGC AACTTAACAA TCTCGCCCTG 
CTCTGCCAGA ATCAGGGCAA ATACGACGAG GTTGAGAAGT ACTACCAGCG AGCTCTCGAC 
ATCTACGAAT CAAAGTTGGG TCCCGATGAT CCCAACGTGG CCAAGACAAA GAATAATCTG 
GCCGGCTGCT ATCTGAAGCA AGGTAGATAC ACCGAAGCTG AAATCCTCTA TAAGCAGGTC 
TTGACGCGAG CCCACGAACG TGAGTTTGGA GCCATCGACA GCAAAAACAA GCCCATTTGG 
CAGGTGGCTG AGGAGCGTGA GGAGCACAAA TTCGATAACA GGGAGAACAC TCCATATGGC 
GAGTACGGCG GTTGGCATAA GGCCGCTAAA GTGGATTCGC CCACGGTCAC AACCACTCTA 
AAAAATCTGG GAGCACTTTA CCGACGTCAA GGCATGTTTG AAGCGGCCGA AACCCTGGAA 
GACTGTGCAA TGCGGAGTAA AAAAGAAGCC TACGATCTAG CTAAACAAAC GAAGCTCTCA 
CAATTGCTAA CGTCAAACGA AAAGCGACGA TCGAAGGCAA TTAAAGAGGA TTTGGATTTC 
TCTGAGGAGA AGAATGCGAA ACCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Klc ( NM_001274851.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261780.1 Drosophila melanogaster kinesin light chain, transcript variant B (Klc), mRNA.

Price & Availability↑

CloneID	OFa18373
Clone ID Related Accession (Same CDS sequence)	NM_079325.3 , NM_001274851.1
Accession Version	NM_001274851.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kinesin light chain, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCAAA TGTCGCAGGA CGAAATCATA ACCAATACGA AAACAGTCCT TCAAGGCCTC 
GAAGCCTTGC GAGTGGAGCA TGTTTCGATT ATGAACGGAA TCGCCGAGGT GCAGAAGGAC 
AATGAAAAGT CGGACATGCT GCGAAAGAAC ATCGAGAACA TCGAACTGGG CCTCAGCGAG 
GCCCAGGTGA TGATGGCCCT GACATCCCAT CTGCAGAACA TCGAGGCCGA GAAGCACAAG 
CTTAAGACAC AGGTGCGCCG CCTCCACCAG GAGAACGCCT GGCTTCGCGA CGAGCTGGCC 
AACACTCAGC AAAAGTTCCA GGCATCCGAG CAGCTGGTGG CCCAACTAGA GGAGGAGAAG 
AAGCACCTGG AGTTCATGGC GTCCGTGAAG AAGTACGACG AGAATCAGGA GCAGGACGAC 
GCTTGCGACA AGTCCCGCAC CGATCCCGTG GTCGAGCTAT TCCCGGACGA GGAAAACGAG 
GACAGACACA ACATGTCGCC CACTCCGCCC AGCCAGTTCG CCAACCAGAC TTCCGGCTAC 
GAGATTCCAG CGCGTCTCCG TACTCTGCAC AACCTGGTCA TTCAGTATGC ATCGCAGGGA 
AGATACGAGG TAGCCGTTCC CCTCTGCAAG CAAGCATTGG AAGATCTCGA AAGGACAAGT 
GGTCACGACC ATCCCGATGT AGCAACCATG CTGAACATTT TGGCTCTCGT GTACCGCGAT 
CAGAACAAGT ATAAGGAGGC CGCCAATTTA CTCAACGACG CTCTGTCGAT TCGAGGAAAG 
ACACTGGGCG AGAACCATCC AGCCGTGGCG GCCACGTTGA ACAACTTGGC CGTCCTCTAT 
GGCAAACGTG GCAAGTACAA AGATGCCGAG CCACTGTGCA AGCGTGCCTT GGAGATCCGA 
GAGAAAGTCC TGGGAAAGGA TCATCCCGAT GTTGCCAAGC AACTTAACAA TCTCGCCCTG 
CTCTGCCAGA ATCAGGGCAA ATACGACGAG GTTGAGAAGT ACTACCAGCG AGCTCTCGAC 
ATCTACGAAT CAAAGTTGGG TCCCGATGAT CCCAACGTGG CCAAGACAAA GAATAATCTG 
GCCGGCTGCT ATCTGAAGCA AGGTAGATAC ACCGAAGCTG AAATCCTCTA TAAGCAGGTC 
TTGACGCGAG CCCACGAACG TGAGTTTGGA GCCATCGACA GCAAAAACAA GCCCATTTGG 
CAGGTGGCTG AGGAGCGTGA GGAGCACAAA TTCGATAACA GGGAGAACAC TCCATATGGC 
GAGTACGGCG GTTGGCATAA GGCCGCTAAA GTGGATTCGC CCACGGTCAC AACCACTCTA 
AAAAATCTGG GAGCACTTTA CCGACGTCAA GGCATGTTTG AAGCGGCCGA AACCCTGGAA 
GACTGTGCAA TGCGGAGTAA AAAAGAAGCC TACGATCTAG CTAAACAAAC GAAGCTCTCA 
CAATTGCTAA CGTCAAACGA AAAGCGACGA TCGAAGGCAA TTAAAGAGGA TTTGGATTTC 
TCTGAGGAGA AGAATGCGAA ACCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261780.1
CDS	118..1644
Translation	MTQMSQDEIITNTKTVLQGLEALRVEHVSIMNGIAEVQKDNEKSDMLRKNIENIELGLSEAQVMMALTSHLQNIEAEKHKLKTQVRRLHQENAWLRDELANTQQKFQASEQLVAQLEEEKKHLEFMASVKKYDENQEQDDACDKSRTDPVVELFPDEENEDRHNMSPTPPSQFANQTSGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLERTSGHDHPDVATMLNILALVYRDQNKYKEAANLLNDALSIRGKTLGENHPAVAATLNNLAVLYGKRGKYKDAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYDEVEKYYQRALDIYESKLGPDDPNVAKTKNNLAGCYLKQGRYTEAEILYKQVLTRAHEREFGAIDSKNKPIWQVAEEREEHKFDNRENTPYGEYGGWHKAAKVDSPTVTTTLKNLGALYRRQGMFEAAETLEDCAMRSKKEAYDLAKQTKLSQLLTSNEKRRSKAIKEDLDFSEEKNAKP

Target ORF information:

RefSeq Version	NM_001274851.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kinesin light chain, transcript variant B (Klc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274851.1

ORF Insert Sequence:

ATGACGCAAA TGTCGCAGGA CGAAATCATA ACCAATACGA AAACAGTCCT TCAAGGCCTC 
GAAGCCTTGC GAGTGGAGCA TGTTTCGATT ATGAACGGAA TCGCCGAGGT GCAGAAGGAC 
AATGAAAAGT CGGACATGCT GCGAAAGAAC ATCGAGAACA TCGAACTGGG CCTCAGCGAG 
GCCCAGGTGA TGATGGCCCT GACATCCCAT CTGCAGAACA TCGAGGCCGA GAAGCACAAG 
CTTAAGACAC AGGTGCGCCG CCTCCACCAG GAGAACGCCT GGCTTCGCGA CGAGCTGGCC 
AACACTCAGC AAAAGTTCCA GGCATCCGAG CAGCTGGTGG CCCAACTAGA GGAGGAGAAG 
AAGCACCTGG AGTTCATGGC GTCCGTGAAG AAGTACGACG AGAATCAGGA GCAGGACGAC 
GCTTGCGACA AGTCCCGCAC CGATCCCGTG GTCGAGCTAT TCCCGGACGA GGAAAACGAG 
GACAGACACA ACATGTCGCC CACTCCGCCC AGCCAGTTCG CCAACCAGAC TTCCGGCTAC 
GAGATTCCAG CGCGTCTCCG TACTCTGCAC AACCTGGTCA TTCAGTATGC ATCGCAGGGA 
AGATACGAGG TAGCCGTTCC CCTCTGCAAG CAAGCATTGG AAGATCTCGA AAGGACAAGT 
GGTCACGACC ATCCCGATGT AGCAACCATG CTGAACATTT TGGCTCTCGT GTACCGCGAT 
CAGAACAAGT ATAAGGAGGC CGCCAATTTA CTCAACGACG CTCTGTCGAT TCGAGGAAAG 
ACACTGGGCG AGAACCATCC AGCCGTGGCG GCCACGTTGA ACAACTTGGC CGTCCTCTAT 
GGCAAACGTG GCAAGTACAA AGATGCCGAG CCACTGTGCA AGCGTGCCTT GGAGATCCGA 
GAGAAAGTCC TGGGAAAGGA TCATCCCGAT GTTGCCAAGC AACTTAACAA TCTCGCCCTG 
CTCTGCCAGA ATCAGGGCAA ATACGACGAG GTTGAGAAGT ACTACCAGCG AGCTCTCGAC 
ATCTACGAAT CAAAGTTGGG TCCCGATGAT CCCAACGTGG CCAAGACAAA GAATAATCTG 
GCCGGCTGCT ATCTGAAGCA AGGTAGATAC ACCGAAGCTG AAATCCTCTA TAAGCAGGTC 
TTGACGCGAG CCCACGAACG TGAGTTTGGA GCCATCGACA GCAAAAACAA GCCCATTTGG 
CAGGTGGCTG AGGAGCGTGA GGAGCACAAA TTCGATAACA GGGAGAACAC TCCATATGGC 
GAGTACGGCG GTTGGCATAA GGCCGCTAAA GTGGATTCGC CCACGGTCAC AACCACTCTA 
AAAAATCTGG GAGCACTTTA CCGACGTCAA GGCATGTTTG AAGCGGCCGA AACCCTGGAA 
GACTGTGCAA TGCGGAGTAA AAAAGAAGCC TACGATCTAG CTAAACAAAC GAAGCTCTCA 
CAATTGCTAA CGTCAAACGA AAAGCGACGA TCGAAGGCAA TTAAAGAGGA TTTGGATTTC 
TCTGAGGAGA AGAATGCGAA ACCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Klc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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