CG5645 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG5645
Entrez Gene ID	39382
Full Name	CG5645 gene product from transcript CG5645-RA
Synonyms	Dmel\CG5645
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 68F5-68F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_140293.3	NP_648550.1	uncharacterized protein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	KRI1	XP_003316159.1
Rattus norvegicus (Norway rat)	LOC687183	XP_006242752.1
Drosophila melanogaster (fruit fly)	CG5645	NP_648550.1
Canis lupus familiaris (dog)	KRI1	XP_542068.2
Danio rerio (zebrafish)	kri1	NP_001002041.1
Xenopus tropicalis (tropical clawed frog)	kri1	XP_002939718.1
Homo sapiens (human)	KRI1	NP_075384.3
Mus musculus (house mouse)	Kri1	NP_663391.2
Caenorhabditis elegans (roundworm)	F54C9.9	NP_495815.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007295	XP_308526.4
Bos taurus (cattle)	KRI1	NP_001103542.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG5645 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG5645 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18318	NM_140293.3 Latest version!	Drosophila melanogaster uncharacterized protein (CG5645), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG5645 ( NM_140293.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648550.1 Drosophila melanogaster uncharacterized protein (CG5645), mRNA.

Price & Availability↑

CloneID	OFa18318
Clone ID Related Accession (Same CDS sequence)	NM_140293.3
Accession Version	NM_140293.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140293.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATAATA ATCTTTTTGA GGGCTCCGAC GACGATGGGG AGGATCTGCA GCTGTCCACG 
AACAAGGACT ATGCCAAGAC CTACAACATC CTGCGCAAGA AAGAGCTACT ACAGAAATAT 
AAGGATCGTG GCTTGGATGT TTCCGAATCG GAATTCGACA GTGACAGCTC GTCATCCGAG 
GAGGACGAGG TGGACCCCAA ATTCGACCAG GACTTCTTCA AGACCCTGTC CTCGCTGAAA 
AGTAAAGACC CATGTATCTA CGACAAGGGT ACGAAATTCT TCAGTGAGTC CTCCGGCGAT 
GAAGATGATA AGGATGGGGA GGCTCCGAAA AAGAAGAAAA AAGCCAAGCC AGTCACTTTG 
AAGGACTACG AACGAAAGGT AATTTTGGAG CATAATGGCA AGTTCGAGAG TTCAGATGAG 
GAGCAGCAGG AAAAGGAGCA CGAGGAACTC CAACGTGCGC AGTCACCGAG CGCCGTGGAG 
GAGGAGCGCC GTCTAAAGGC CGAGTTCCGG AAGGTGATGA ACAAGGAAGA CGACAGTGAG 
GATGAGGAAT TTGGAGGTAT ATTCAAGAAG CGCAGTAAGA CCAAGGAGCA AACAGCTGCC 
GAGGAGGCAG ACTTTGCCAA ATGGTTGGCG GGAAAACAAG CTGAGATTCA AGAAACCGAC 
AAGAAGCAGT TGGAGCCTCT AAAACAGTAC TGGAGCAGCA ACAAGCTGAC CCAGGGCGAG 
AGTTTCCTCA GGGATTACAT TCTCAACAAG GGCTATGCAA ACACGGATGA GTCCGCCATT 
CCCACCTACG ATGAGATCGT GGGCGAAGCT GCTCCGCTCT CCGAGGATGA GCAGGAACTT 
GAGAAGCAGG CAGAGTTTGA GCACAAGTAC AATTTCCGCT TTGAGGAGCC AGATGCGGAC 
TTCATAAAGC GCTATCCCCG CACCATCGAA CAATCCCTGC GTCGCACGGA TGACAAGAGG 
AAAGAAAAAC GCAAGGAGCT GAAGGAGCGC AAGGATCAGG AGAAACAGCA AAAGATGAAG 
GAGTTGGAAC TGGTCAAGGA AATGAAGCGT AAGGAGATCG ATGAAAAGAT ACGCAAATTA 
AAAGCGGTCA CTGGCAACGA TGAGCTTGGT TTCCGGGACG AAGAACTCGA GGAGGACTTT 
GACCCGGCGG CCCACGATCG TCGAATGCAG GAGCTCTTCG ACGATGAGTA CTACAATGTC 
GATGAAGGCG AGGAGAAACC AGAATGTCCC TCGGATATCG ATGAATTGGT GCTGGAAGAT 
TGGGATAACT ACGATCCTAG ACAGCACGCC AATGGGGGCG GTGAGGATTA TGAAGGACAC 
TGCGAGGACG ACGACTTCAA CATGGATTGC GACTACGATC CGTCCACGGC CAAGGAGCAA 
CTTCAACAAG AGCTCATCGA AAACACTCGT GGTCGCAAAG GACGAAAGGG TAGACGAAAT 
CGCTTTATGG AAATGATCCA GGCGGAAAAG CCTGCATTCA ATCCTGAGGA CGAAAAGACG 
TACAGTGAGT ACATCGATGA GTACTACCAA ATGGATTGCG AGGACATCAT CGGGGATCAA 
CCGTGTCGAT TTAAATATGT GGAAACCACA CCAAACGATT TCGGTTTGAC GATAGAAGAG 
ATCTTGCTGG CCAAGAACAA GGAGCTTAAC CAGTGGGCTA GCTTAAAGAA AGCTGTCCAA 
AACAGACCGG AACACGTAGA GAAAAAGGAG CAGCGTCTCT ACAAGATGAA AGCCAAGAAT 
GAAGACCTTA AGCGAAAGAT ATTTAAGAGT CTTTATGGAG AAGGTTCTGA CGATGAGGAG 
CAGCCAGCAG AAGAGAAACC AGAAGTGACA CCAGCTGAAG CGACAGCTCC AGCCGAAAAT 
GGTCAAGTCT CGACAGAAGG TTTGTCCAAA TCAAAGAGGA AACGCCTGAA ACGTAAGGCT 
GCCGCAGCAG CAGCCAGTGC TCCTAAGGTT CTAAAAGAAG AAAGTGACTC CAAGGATCCA 
AAGGAAGCGG ATGGATCTAC TGAAGATGTC CAGGCAGAAA GTTCTAAGAA AAAAGTCGAT 
ACTCCCTCTA AAAAGGGCAA AGATGACGCA AATCAGGAAA CTAAAAACAG TCCACAAAGC 
ACGGAGAAAA CGAAAAACAA TAATGCCTTA AAAAATAACA AAAAAGAGCC TAAAAACGTG 
CAAAATGGAT TCCAAAAACC CCAAAATCAA GCAAATAAAA GTGCAAAGAC AAAGTCGAAC 
CAACCATTTA AAACTACGGA AAGTGCGCCT GCAAAAGCAG AAAAATCAAA TGGTAATAAT 
CCTTTCAATA AGCCACAATC GAAATCCCAA CAAAGGCAGG AACTGCCACC AATTCATAAA 
AATCAAGGTG GAAACAAAAA GGGACCGAGA AACGCTAACG GCACCAATCC CTTTAAGAAA 
TCAAACCAAA AGCCAAGTGC ACCCTTCCCA GCTAAGAAAA CGAACAACTT TAAGGCGAAA 
AACAAGCAAA ATAACAATAG TGGAATAACC GACGATCGAT TAAAGGCATA TGGCATAAAT 
CCCAGAAAAT TCCACAAGCG GGAAAAATAT GGAAAAAAGG ATAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648550.1
CDS	73..2640
Translation	MNNNLFEGSDDDGEDLQLSTNKDYAKTYNILRKKELLQKYKDRGLDVSESEFDSDSSSSEEDEVDPKFDQDFFKTLSSLKSKDPCIYDKGTKFFSESSGDEDDKDGEAPKKKKKAKPVTLKDYERKVILEHNGKFESSDEEQQEKEHEELQRAQSPSAVEEERRLKAEFRKVMNKEDDSEDEEFGGIFKKRSKTKEQTAAEEADFAKWLAGKQAEIQETDKKQLEPLKQYWSSNKLTQGESFLRDYILNKGYANTDESAIPTYDEIVGEAAPLSEDEQELEKQAEFEHKYNFRFEEPDADFIKRYPRTIEQSLRRTDDKRKEKRKELKERKDQEKQQKMKELELVKEMKRKEIDEKIRKLKAVTGNDELGFRDEELEEDFDPAAHDRRMQELFDDEYYNVDEGEEKPECPSDIDELVLEDWDNYDPRQHANGGGEDYEGHCEDDDFNMDCDYDPSTAKEQLQQELIENTRGRKGRKGRRNRFMEMIQAEKPAFNPEDEKTYSEYIDEYYQMDCEDIIGDQPCRFKYVETTPNDFGLTIEEILLAKNKELNQWASLKKAVQNRPEHVEKKEQRLYKMKAKNEDLKRKIFKSLYGEGSDDEEQPAEEKPEVTPAEATAPAENGQVSTEGLSKSKRKRLKRKAAAAAASAPKVLKEESDSKDPKEADGSTEDVQAESSKKKVDTPSKKGKDDANQETKNSPQSTEKTKNNNALKNNKKEPKNVQNGFQKPQNQANKSAKTKSNQPFKTTESAPAKAEKSNGNNPFNKPQSKSQQRQELPPIHKNQGGNKKGPRNANGTNPFKKSNQKPSAPFPAKKTNNFKAKNKQNNNSGITDDRLKAYGINPRKFHKREKYGKKDN

Target ORF information:

RefSeq Version	NM_140293.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein (CG5645), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140293.3

ORF Insert Sequence:

ATGAATAATA ATCTTTTTGA GGGCTCCGAC GACGATGGGG AGGATCTGCA GCTGTCCACG 
AACAAGGACT ATGCCAAGAC CTACAACATC CTGCGCAAGA AAGAGCTACT ACAGAAATAT 
AAGGATCGTG GCTTGGATGT TTCCGAATCG GAATTCGACA GTGACAGCTC GTCATCCGAG 
GAGGACGAGG TGGACCCCAA ATTCGACCAG GACTTCTTCA AGACCCTGTC CTCGCTGAAA 
AGTAAAGACC CATGTATCTA CGACAAGGGT ACGAAATTCT TCAGTGAGTC CTCCGGCGAT 
GAAGATGATA AGGATGGGGA GGCTCCGAAA AAGAAGAAAA AAGCCAAGCC AGTCACTTTG 
AAGGACTACG AACGAAAGGT AATTTTGGAG CATAATGGCA AGTTCGAGAG TTCAGATGAG 
GAGCAGCAGG AAAAGGAGCA CGAGGAACTC CAACGTGCGC AGTCACCGAG CGCCGTGGAG 
GAGGAGCGCC GTCTAAAGGC CGAGTTCCGG AAGGTGATGA ACAAGGAAGA CGACAGTGAG 
GATGAGGAAT TTGGAGGTAT ATTCAAGAAG CGCAGTAAGA CCAAGGAGCA AACAGCTGCC 
GAGGAGGCAG ACTTTGCCAA ATGGTTGGCG GGAAAACAAG CTGAGATTCA AGAAACCGAC 
AAGAAGCAGT TGGAGCCTCT AAAACAGTAC TGGAGCAGCA ACAAGCTGAC CCAGGGCGAG 
AGTTTCCTCA GGGATTACAT TCTCAACAAG GGCTATGCAA ACACGGATGA GTCCGCCATT 
CCCACCTACG ATGAGATCGT GGGCGAAGCT GCTCCGCTCT CCGAGGATGA GCAGGAACTT 
GAGAAGCAGG CAGAGTTTGA GCACAAGTAC AATTTCCGCT TTGAGGAGCC AGATGCGGAC 
TTCATAAAGC GCTATCCCCG CACCATCGAA CAATCCCTGC GTCGCACGGA TGACAAGAGG 
AAAGAAAAAC GCAAGGAGCT GAAGGAGCGC AAGGATCAGG AGAAACAGCA AAAGATGAAG 
GAGTTGGAAC TGGTCAAGGA AATGAAGCGT AAGGAGATCG ATGAAAAGAT ACGCAAATTA 
AAAGCGGTCA CTGGCAACGA TGAGCTTGGT TTCCGGGACG AAGAACTCGA GGAGGACTTT 
GACCCGGCGG CCCACGATCG TCGAATGCAG GAGCTCTTCG ACGATGAGTA CTACAATGTC 
GATGAAGGCG AGGAGAAACC AGAATGTCCC TCGGATATCG ATGAATTGGT GCTGGAAGAT 
TGGGATAACT ACGATCCTAG ACAGCACGCC AATGGGGGCG GTGAGGATTA TGAAGGACAC 
TGCGAGGACG ACGACTTCAA CATGGATTGC GACTACGATC CGTCCACGGC CAAGGAGCAA 
CTTCAACAAG AGCTCATCGA AAACACTCGT GGTCGCAAAG GACGAAAGGG TAGACGAAAT 
CGCTTTATGG AAATGATCCA GGCGGAAAAG CCTGCATTCA ATCCTGAGGA CGAAAAGACG 
TACAGTGAGT ACATCGATGA GTACTACCAA ATGGATTGCG AGGACATCAT CGGGGATCAA 
CCGTGTCGAT TTAAATATGT GGAAACCACA CCAAACGATT TCGGTTTGAC GATAGAAGAG 
ATCTTGCTGG CCAAGAACAA GGAGCTTAAC CAGTGGGCTA GCTTAAAGAA AGCTGTCCAA 
AACAGACCGG AACACGTAGA GAAAAAGGAG CAGCGTCTCT ACAAGATGAA AGCCAAGAAT 
GAAGACCTTA AGCGAAAGAT ATTTAAGAGT CTTTATGGAG AAGGTTCTGA CGATGAGGAG 
CAGCCAGCAG AAGAGAAACC AGAAGTGACA CCAGCTGAAG CGACAGCTCC AGCCGAAAAT 
GGTCAAGTCT CGACAGAAGG TTTGTCCAAA TCAAAGAGGA AACGCCTGAA ACGTAAGGCT 
GCCGCAGCAG CAGCCAGTGC TCCTAAGGTT CTAAAAGAAG AAAGTGACTC CAAGGATCCA 
AAGGAAGCGG ATGGATCTAC TGAAGATGTC CAGGCAGAAA GTTCTAAGAA AAAAGTCGAT 
ACTCCCTCTA AAAAGGGCAA AGATGACGCA AATCAGGAAA CTAAAAACAG TCCACAAAGC 
ACGGAGAAAA CGAAAAACAA TAATGCCTTA AAAAATAACA AAAAAGAGCC TAAAAACGTG 
CAAAATGGAT TCCAAAAACC CCAAAATCAA GCAAATAAAA GTGCAAAGAC AAAGTCGAAC 
CAACCATTTA AAACTACGGA AAGTGCGCCT GCAAAAGCAG AAAAATCAAA TGGTAATAAT 
CCTTTCAATA AGCCACAATC GAAATCCCAA CAAAGGCAGG AACTGCCACC AATTCATAAA 
AATCAAGGTG GAAACAAAAA GGGACCGAGA AACGCTAACG GCACCAATCC CTTTAAGAAA 
TCAAACCAAA AGCCAAGTGC ACCCTTCCCA GCTAAGAAAA CGAACAACTT TAAGGCGAAA 
AACAAGCAAA ATAACAATAG TGGAATAACC GACGATCGAT TAAAGGCATA TGGCATAAAT 
CCCAGAAAAT TCCACAAGCG GGAAAAATAT GGAAAAAAGG ATAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG5645 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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