rols cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following rols gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rols cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa18305 NM_001274810.1
Latest version!
Drosophila melanogaster rolling pebbles (rols), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa18306 NM_001274808.1
Latest version!
Drosophila melanogaster rolling pebbles (rols), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa19983 NM_168487.2
Latest version!
Drosophila melanogaster rolling pebbles (rols), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa18307 NM_001274809.1
Latest version!
Drosophila melanogaster rolling pebbles (rols), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OFa18308 NM_001274807.1
Latest version!
Drosophila melanogaster rolling pebbles (rols), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OFa18309 NM_140285.3
Latest version!
Drosophila melanogaster rolling pebbles (rols), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OFa18309 NM_168488.2
Latest version!
Drosophila melanogaster rolling pebbles (rols), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OFa19939 NM_168489.2
Latest version!
Drosophila melanogaster rolling pebbles (rols), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OFa19939 NM_168490.2
Latest version!
Drosophila melanogaster rolling pebbles (rols), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa18305
    Clone ID Related Accession (Same CDS sequence) NM_001274810.1
    Accession Version NM_001274810.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5397bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product rolling pebbles, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCCCTCGC TGCAGCAAAT TGCGCTCGCG ATGGAAGGTG ACCAATCGGA GCTACAGACA 
    CCGCCGCTGC CGATCAACCC GCCGCCCAAG GTGATGGCCA ATGGAATCGT CTATGGCTTT
    CGCACCCGAC ACTTGTCCTG CATCGAGGAG AACGACTCGG ACTCGTTGCA CGGATCGTCC
    ATTCCACATG CCTACGATAT GATGCAGCAG GCGGACGAGA CCTTCCAGAA GTTGAATGCC
    AACGAGGAGC AGGACGAGTC GGGTAGCGAG GATGGGGGAT TGGAGAATCT GGACGATACG
    ATCGCAATGA TCGATGAATC GCGCCTTTTG GAGGCCGATT ATCTGGATGA GCTCGTGGCC
    GAGGAGCAGT TGCAGGAGCA GGACGAGGAT GAGGAGGAAT CCTCTGATCT TGTCGACTCT
    GGCAATGTGG AGGAGGATTT CTGGCAGCCG AGCTTCAATC GGAATGACAT CATGATGACC
    TCGGTTTATG GAGCTCTGAA TGGCTCGTTG ACGAGCGAAG TGTCTTCAAC TGCAGCGCCG
    GGCTACTCCG AGTGTGCCAC GCCCAGAAAG GAGGCGGAAC CCGTTTATGC CACGCCGGAA
    AAGCGACGAG CCAGCAATCG GAGTTTCGAC TCCACCTGCG CCTCGCTCAC CAGTTCCTTA
    TACGGCGGAT CTATGAACTC CTCGCTGGAT CTCAAGTACT CATCCTTGGG TGGTGGCGAC
    TCGGTCAGTG GTGGCATTAG TGGAACCAGC ACTTTGCGAG GAACTTCAGT GCCGCCAATG
    GATCTGTCCA TGATATCCAG CGATGGCGTT AGTGCGCTGG ACTGGGGATC TGCCAGTGTG
    AGCTGTGTTT TGGACTCCGG CGATGGGAAT CCCGCCGAGG AGCTCAACGA GGAAATGGCC
    GCCAAGTGCT TGAGTGCAGC GGAAAGTGAT CTCCAGTCCA TCCGACGACT CCTGGAGCAC
    GATGCCAGTG GATCCGTTTG CCCCTCCTGC CGCATATCCT TCGACAAGGG CAAGAGACGG
    AAACTGATCG ACACCTGCGG CCACGAGCGG TGCTATTCCT GCATGTTCCG CAACGACCAG
    TGCCCCATGT GCATGAACAG CTCGCTGAAA GATGTTGATG GCGCCAATGC TCAGGGCTAT
    GACACCGGAA TCGGTGGCTC CACATCGACG ATAGTGAGTC CTCTTGGATC CCCGCAGCCG
    CAGCTCAGAT CGCATGCGCA ACGAAACGCA GCGCTGGCGC GCTACATGCA GCACCGCCAG
    GAGTCGCAGT CGCCAGATTA CCACCATCGC TATGCGAGCA ATGGCAAACT GCCCCGGGCG
    GCGGCACTGT CTACCAATGG CAATGGATTT ACCACCGGCA CCACGCAAAC GGTCACAGTG
    GATGTGCATC ACCAGCTCGG CGGAGGAGGA GGAGGTGCTG CCGCCGCGGC CAAGCAGTTG
    ATGGCCAACG GCATCAGTGG CCATAGTCGC AGCAGCAGCA TGAGCCACAA CATCCATGCG
    GCAGCCTACA GCGAACTGAG TGCCGCACCA CCGGCCTTCG CCACACCGCC GACGCGTAGG
    CGGTTCTTTA ACCACAAGAA TCTGCGCAGT GCCCTCACTG GAGGAAGTGG CGGTGGATCG
    GGAGTCGGCG GTGGTGTAGG AGGAGCTGGC GGTGTGGGCG GCGGTCATCG ACGCACTGCC
    TCCAATGGGG GTTGTCCCAT TGACACAGCA TCGATCCTGT CGGGAGGAAG CGACGCTATT
    GACCACCACT CCTCCTTCCT GAATGAGAAA CTGCAGAAGG ATCAGCTCCA CGCCCGTCTC
    GGTCTGCTGC TCAACGATCC CGGCAGCAAT GGAAACAGCA GCAGCAGCGG AAGTGGTTGT
    GAACCCATCT CGGCCCATTC CACCACCAGC ACCACGAGCA GCAGCGGCGT GGGAGCGGCC
    AGCACCACCA CGAGCGGTTC CTCGCAGAAT GTCAGTCCGG AGCAAACGCT GGCCAGCGGA
    GCCGGAATGC TCAGTGGCAG TCAGCTGTCG GTGGCCACCA GTCATGGGGT CAAGGAGGAT
    GCACTGAGTC TGTGCGGCAA GTTCAAGGCT GGCTGCTCCA TGCTGCACGT CTACGAAGCA
    TTGCCCAGCA AGAGTCGCAA AGGAAATGTA CGGAGATCGA CGCGTGGTCA GCAGGGATCC
    AGTTCGAGCG CATCGGCGGC ATCAGCAGTG GGATCCAGGG TGACAGCCTC CAGTTTGGCG
    GCTGTGCAGT TGGCCCTGAA GCCACTGTTC TTTGAGGTGC CGCTGCAGGA ACCGGATCCA
    CCATATGTGG GTCGCCAGTG GCTGGTGCAG CAGTTGTCCA ACATCCTGCT TGGCACCGAG
    ACACGGGTGG TGCTCATTAA CGGACAGCCG GGCACTGGAA AGACGGCGTT TTGTCTGCAG
    CTGGTGGAGT ACTCCTGCTT TGGCCGCCGG CAGATGCAGG ATGATCCCGA TGGCATATAC
    AGTCAGCTAC AGTTGGGCGC CCACTGTGAG CGCATGCGCG GACTGGCCTC CCACATGGTT
    GGCTATCACT TCTGCCAGGC GGACGCCAAT CTCACCTGCC AGGTGCCCGA TTTCGTGCAT
    TCTCTGGCCG CCCAACTGTG TCAGGCGCCG CAGTTAACCG CCTACAGGGA TTACCTGCTC
    TCGGAGCCGC ATCTTCAGGA CATTCTCAGT GTGCGAGAGT GCATCGCGGA TGCGGAGAGA
    GTAATGAAGC TGGCTATACT GGAACCACTG GCTCACCTTC ACCGGGCGGG AAAGATACCC
    GCCAAAGTGG CCGTCATCGT GGTGGATGCC TTGTGCGAAG CGGAGTACCA TCGTCCCGAT
    CATGGTCACA CCATTGCTAG TTTCTTGGCA CAGCTTACGC CACATTTTCC AGCTTGGCTC
    AAGCTGGTGG CCACTGTGAG GACCCAGATG TTGGAGCTGG TGAAGGCACC CAGCTACACA
    CAACTTACCC TGGACAGTTG GGCCAGCAGC CAGGCGTTGC AGCAGGATAT GCTTGATTAC
    ATAGGCGCTC GGCTTGCAGA TAGTCCGGAA ATTCGAATGA ACATCGGCGG CGGAGGAGGC
    CAGAACTCGC AGTCGGGCAG CCAGCCGCAG ACGAAGTTCG TGTCCCATCT TCAGTCCCTG
    AGCAGGGGCT CCATGCTGTA CGCTAAGCTC ATCCTGGATC TCATTGCACG CGGCCAGTTG
    GTTATCAAGT CATCCAGCTA CAAGGTGCTG CCGGTCTCTC TTGCTCAGAT CTTTCTGCTG
    CACTTCAACC TGCGCTTTCC CACCGCCCGT AGCTTTGAGC AGGCTGCTCC GATCCTCAAT
    ATATGCCTGG CTGCTCTGTA TCCTCTGACG CTGGACGAGA TCTACTACAG TATGGAGGCA
    CTGAGTCACG GACGGGAGGC ACTAAGCTGG CCGGACTTCA TGCAACGCTT TAAGCTGCTG
    GATGGATTTT TAATCAAGAG GTTGGACAAC ACGTACATGT TCTTTCATAG CTCCTTGAGG
    GAGTGGCTCA TGCGAAGGGA TGAGGGGGAG TCCAACAAGT TCCTCTGCGA CGCCCGTTTG
    GGTCATGCTG GAATCGCTTT CCGATTATCC CGACTGCAGG CTCCATTGTC ACCTCAGCTC
    ACATTGGAAC TGGGTCACCA CATGCTGAAA GCACATTTAT ACGGAGGAAC TAGTCTGACG
    CTGCTTTCTC CGCGGGATTT GCAATCTTAT TGGTTGGCGG GAGCGGCGGA TAACATTTCA
    TCCTCGCTGG GCGCCCTGCG AAATGTCTAC AGTCCGAACC TAAAGGTTTC CCGACTAGTT
    CTCTTGGCAG GAGCTTCACC GAATCACCGC ACGGATTACA TGGGTGGAGC TCCGATCCTT
    TGCATAGCTG CGCATGAGGG AATCCTTCCC ATGGTTAGCC TGCTCCTAGA ATTCGGTGCC
    GATGTCGGTC TGACCAACAG CCAGGGCTGT ACTCCATTAA TACTGGCCGC CATGCGTGGC
    CACTGCGACG TAGTGCGTCC ATTGGTAGCC GCCGGCAGCA GCCTCGGTCA GTTGGACATC
    ACGCAGCGCT GTGCCCTAGT TCATGCCGCC CGCATGGGTC ACCTCAGTGT GGTCAAGTAC
    TTGCTCGCCT GCGACTGGTC ACCACGACCG CACTCCCAAG ATGTCACCAG ATCGGTTGCC
    TTGCAACAGG CGCTCATTGG AGCTGCCGCA CAGGCGCACT GCAAGATCCT TGAGGATCTT
    CTGGATCTCA ATGAGACCGA GTTTGACCTG GACGTGAATG GCATGGAGCC AAGCAGTGGT
    GAACTGGCGC TAACCGCCGC CGCACGGCAC GGATGCATTG ATGTTGTAGG AATCTTGCTC
    TCCCGTGGCG CTCAGATCGA TGCAAGAAAT CGGCAGGGAT ACTCGGCGCT TTGGTTGGCT
    GTCAAGGAGG GTCACTGGAG TGTGGTGGAG CATCTGCTGC AACGTGGTGC CCTGTTGGAT
    GAACCATTGG GACAGACACG CAAGACGCCG CTAATGATTG CGGCGGAGGA GGGACACCTG
    GAGCTGGTCG ATCTGCTGCT GGCGCGGGGA GCGCAACGAG AAGCTCAGGA TCACGAGGGA
    TTCACTGCAC TCAGCTGGGC CTGTTTACGT GGCCACTTGG CGGCGGCCAA GACGCTCATA
    GAACATGGCT GTAATCGCCA CCACGAGGAT CACAATGGAC GCACAGCCCT GGATCTGGCA
    GCCTATCAGG GAGCCGCTAG CCTGGTGATC TATATCCTGG AGCAGGGCGG CAATCTGGAA
    CACATTGATG TTCATGGAAT GCGTCCCCTG GATCGGGCCA TTGCCTGCCG TAACATCCAG
    GCCGTACAGG TGTTCCTGCG CAAGGGGGCC AAACTGGGAC CGACCACTTG GAGCATGGCC
    ATGGGCAAGC CGGAGATTCT GGTCATACTA CTGAACAAGC TGCTCGAGGA CGGTAATGTG
    CTGTACCGGA AAAACCGCTT CCAGGAGGCT GCTCATCGCT ATCAGTATGC CCTACGCAAG
    ATCTCCGGAC TGGAGCAGTT GCTCGAAAGG AATGCCATCT TCGCCCAGCT GCGGACCAAT
    CTTCTGCTGA ATCTGTCGCG CTGCAAGCGA AAACTGAATG AACTTGACGC CTCCATAGAT
    TTGGCCACGC AAGCGATTGC CCAGAAGCCG CACAGCTACG AAGGATACTA CGCCAGGGCA
    AAGGCGCGCA TGGAACTTGG TGCATTGAAT GAAGCACTTG TGGACGCCAA TGAGGCGATG
    CAGCAGGCCG CCCAAAGCGG TGTGCTCTGC GAAGTGGTCG AGGTGCTGAA GCGCATCCAG
    ACCGAGCTGC TCACGCGGAT CAGCATCAGC AGCGAGGATC AGACCGGCAG GATGTCGAAT
    ATCGGCGGCG GGGCATCCGT CGCATACGAA TCTCATCATG AGATCACCGA TTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261739.1
    CDS300..5696
    Translation

    Target ORF information:

    RefSeq Version NM_001274810.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster rolling pebbles (rols), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001274810.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCCCTCGC TGCAGCAAAT TGCGCTCGCG ATGGAAGGTG ACCAATCGGA GCTACAGACA 
    CCGCCGCTGC CGATCAACCC GCCGCCCAAG GTGATGGCCA ATGGAATCGT CTATGGCTTT
    CGCACCCGAC ACTTGTCCTG CATCGAGGAG AACGACTCGG ACTCGTTGCA CGGATCGTCC
    ATTCCACATG CCTACGATAT GATGCAGCAG GCGGACGAGA CCTTCCAGAA GTTGAATGCC
    AACGAGGAGC AGGACGAGTC GGGTAGCGAG GATGGGGGAT TGGAGAATCT GGACGATACG
    ATCGCAATGA TCGATGAATC GCGCCTTTTG GAGGCCGATT ATCTGGATGA GCTCGTGGCC
    GAGGAGCAGT TGCAGGAGCA GGACGAGGAT GAGGAGGAAT CCTCTGATCT TGTCGACTCT
    GGCAATGTGG AGGAGGATTT CTGGCAGCCG AGCTTCAATC GGAATGACAT CATGATGACC
    TCGGTTTATG GAGCTCTGAA TGGCTCGTTG ACGAGCGAAG TGTCTTCAAC TGCAGCGCCG
    GGCTACTCCG AGTGTGCCAC GCCCAGAAAG GAGGCGGAAC CCGTTTATGC CACGCCGGAA
    AAGCGACGAG CCAGCAATCG GAGTTTCGAC TCCACCTGCG CCTCGCTCAC CAGTTCCTTA
    TACGGCGGAT CTATGAACTC CTCGCTGGAT CTCAAGTACT CATCCTTGGG TGGTGGCGAC
    TCGGTCAGTG GTGGCATTAG TGGAACCAGC ACTTTGCGAG GAACTTCAGT GCCGCCAATG
    GATCTGTCCA TGATATCCAG CGATGGCGTT AGTGCGCTGG ACTGGGGATC TGCCAGTGTG
    AGCTGTGTTT TGGACTCCGG CGATGGGAAT CCCGCCGAGG AGCTCAACGA GGAAATGGCC
    GCCAAGTGCT TGAGTGCAGC GGAAAGTGAT CTCCAGTCCA TCCGACGACT CCTGGAGCAC
    GATGCCAGTG GATCCGTTTG CCCCTCCTGC CGCATATCCT TCGACAAGGG CAAGAGACGG
    AAACTGATCG ACACCTGCGG CCACGAGCGG TGCTATTCCT GCATGTTCCG CAACGACCAG
    TGCCCCATGT GCATGAACAG CTCGCTGAAA GATGTTGATG GCGCCAATGC TCAGGGCTAT
    GACACCGGAA TCGGTGGCTC CACATCGACG ATAGTGAGTC CTCTTGGATC CCCGCAGCCG
    CAGCTCAGAT CGCATGCGCA ACGAAACGCA GCGCTGGCGC GCTACATGCA GCACCGCCAG
    GAGTCGCAGT CGCCAGATTA CCACCATCGC TATGCGAGCA ATGGCAAACT GCCCCGGGCG
    GCGGCACTGT CTACCAATGG CAATGGATTT ACCACCGGCA CCACGCAAAC GGTCACAGTG
    GATGTGCATC ACCAGCTCGG CGGAGGAGGA GGAGGTGCTG CCGCCGCGGC CAAGCAGTTG
    ATGGCCAACG GCATCAGTGG CCATAGTCGC AGCAGCAGCA TGAGCCACAA CATCCATGCG
    GCAGCCTACA GCGAACTGAG TGCCGCACCA CCGGCCTTCG CCACACCGCC GACGCGTAGG
    CGGTTCTTTA ACCACAAGAA TCTGCGCAGT GCCCTCACTG GAGGAAGTGG CGGTGGATCG
    GGAGTCGGCG GTGGTGTAGG AGGAGCTGGC GGTGTGGGCG GCGGTCATCG ACGCACTGCC
    TCCAATGGGG GTTGTCCCAT TGACACAGCA TCGATCCTGT CGGGAGGAAG CGACGCTATT
    GACCACCACT CCTCCTTCCT GAATGAGAAA CTGCAGAAGG ATCAGCTCCA CGCCCGTCTC
    GGTCTGCTGC TCAACGATCC CGGCAGCAAT GGAAACAGCA GCAGCAGCGG AAGTGGTTGT
    GAACCCATCT CGGCCCATTC CACCACCAGC ACCACGAGCA GCAGCGGCGT GGGAGCGGCC
    AGCACCACCA CGAGCGGTTC CTCGCAGAAT GTCAGTCCGG AGCAAACGCT GGCCAGCGGA
    GCCGGAATGC TCAGTGGCAG TCAGCTGTCG GTGGCCACCA GTCATGGGGT CAAGGAGGAT
    GCACTGAGTC TGTGCGGCAA GTTCAAGGCT GGCTGCTCCA TGCTGCACGT CTACGAAGCA
    TTGCCCAGCA AGAGTCGCAA AGGAAATGTA CGGAGATCGA CGCGTGGTCA GCAGGGATCC
    AGTTCGAGCG CATCGGCGGC ATCAGCAGTG GGATCCAGGG TGACAGCCTC CAGTTTGGCG
    GCTGTGCAGT TGGCCCTGAA GCCACTGTTC TTTGAGGTGC CGCTGCAGGA ACCGGATCCA
    CCATATGTGG GTCGCCAGTG GCTGGTGCAG CAGTTGTCCA ACATCCTGCT TGGCACCGAG
    ACACGGGTGG TGCTCATTAA CGGACAGCCG GGCACTGGAA AGACGGCGTT TTGTCTGCAG
    CTGGTGGAGT ACTCCTGCTT TGGCCGCCGG CAGATGCAGG ATGATCCCGA TGGCATATAC
    AGTCAGCTAC AGTTGGGCGC CCACTGTGAG CGCATGCGCG GACTGGCCTC CCACATGGTT
    GGCTATCACT TCTGCCAGGC GGACGCCAAT CTCACCTGCC AGGTGCCCGA TTTCGTGCAT
    TCTCTGGCCG CCCAACTGTG TCAGGCGCCG CAGTTAACCG CCTACAGGGA TTACCTGCTC
    TCGGAGCCGC ATCTTCAGGA CATTCTCAGT GTGCGAGAGT GCATCGCGGA TGCGGAGAGA
    GTAATGAAGC TGGCTATACT GGAACCACTG GCTCACCTTC ACCGGGCGGG AAAGATACCC
    GCCAAAGTGG CCGTCATCGT GGTGGATGCC TTGTGCGAAG CGGAGTACCA TCGTCCCGAT
    CATGGTCACA CCATTGCTAG TTTCTTGGCA CAGCTTACGC CACATTTTCC AGCTTGGCTC
    AAGCTGGTGG CCACTGTGAG GACCCAGATG TTGGAGCTGG TGAAGGCACC CAGCTACACA
    CAACTTACCC TGGACAGTTG GGCCAGCAGC CAGGCGTTGC AGCAGGATAT GCTTGATTAC
    ATAGGCGCTC GGCTTGCAGA TAGTCCGGAA ATTCGAATGA ACATCGGCGG CGGAGGAGGC
    CAGAACTCGC AGTCGGGCAG CCAGCCGCAG ACGAAGTTCG TGTCCCATCT TCAGTCCCTG
    AGCAGGGGCT CCATGCTGTA CGCTAAGCTC ATCCTGGATC TCATTGCACG CGGCCAGTTG
    GTTATCAAGT CATCCAGCTA CAAGGTGCTG CCGGTCTCTC TTGCTCAGAT CTTTCTGCTG
    CACTTCAACC TGCGCTTTCC CACCGCCCGT AGCTTTGAGC AGGCTGCTCC GATCCTCAAT
    ATATGCCTGG CTGCTCTGTA TCCTCTGACG CTGGACGAGA TCTACTACAG TATGGAGGCA
    CTGAGTCACG GACGGGAGGC ACTAAGCTGG CCGGACTTCA TGCAACGCTT TAAGCTGCTG
    GATGGATTTT TAATCAAGAG GTTGGACAAC ACGTACATGT TCTTTCATAG CTCCTTGAGG
    GAGTGGCTCA TGCGAAGGGA TGAGGGGGAG TCCAACAAGT TCCTCTGCGA CGCCCGTTTG
    GGTCATGCTG GAATCGCTTT CCGATTATCC CGACTGCAGG CTCCATTGTC ACCTCAGCTC
    ACATTGGAAC TGGGTCACCA CATGCTGAAA GCACATTTAT ACGGAGGAAC TAGTCTGACG
    CTGCTTTCTC CGCGGGATTT GCAATCTTAT TGGTTGGCGG GAGCGGCGGA TAACATTTCA
    TCCTCGCTGG GCGCCCTGCG AAATGTCTAC AGTCCGAACC TAAAGGTTTC CCGACTAGTT
    CTCTTGGCAG GAGCTTCACC GAATCACCGC ACGGATTACA TGGGTGGAGC TCCGATCCTT
    TGCATAGCTG CGCATGAGGG AATCCTTCCC ATGGTTAGCC TGCTCCTAGA ATTCGGTGCC
    GATGTCGGTC TGACCAACAG CCAGGGCTGT ACTCCATTAA TACTGGCCGC CATGCGTGGC
    CACTGCGACG TAGTGCGTCC ATTGGTAGCC GCCGGCAGCA GCCTCGGTCA GTTGGACATC
    ACGCAGCGCT GTGCCCTAGT TCATGCCGCC CGCATGGGTC ACCTCAGTGT GGTCAAGTAC
    TTGCTCGCCT GCGACTGGTC ACCACGACCG CACTCCCAAG ATGTCACCAG ATCGGTTGCC
    TTGCAACAGG CGCTCATTGG AGCTGCCGCA CAGGCGCACT GCAAGATCCT TGAGGATCTT
    CTGGATCTCA ATGAGACCGA GTTTGACCTG GACGTGAATG GCATGGAGCC AAGCAGTGGT
    GAACTGGCGC TAACCGCCGC CGCACGGCAC GGATGCATTG ATGTTGTAGG AATCTTGCTC
    TCCCGTGGCG CTCAGATCGA TGCAAGAAAT CGGCAGGGAT ACTCGGCGCT TTGGTTGGCT
    GTCAAGGAGG GTCACTGGAG TGTGGTGGAG CATCTGCTGC AACGTGGTGC CCTGTTGGAT
    GAACCATTGG GACAGACACG CAAGACGCCG CTAATGATTG CGGCGGAGGA GGGACACCTG
    GAGCTGGTCG ATCTGCTGCT GGCGCGGGGA GCGCAACGAG AAGCTCAGGA TCACGAGGGA
    TTCACTGCAC TCAGCTGGGC CTGTTTACGT GGCCACTTGG CGGCGGCCAA GACGCTCATA
    GAACATGGCT GTAATCGCCA CCACGAGGAT CACAATGGAC GCACAGCCCT GGATCTGGCA
    GCCTATCAGG GAGCCGCTAG CCTGGTGATC TATATCCTGG AGCAGGGCGG CAATCTGGAA
    CACATTGATG TTCATGGAAT GCGTCCCCTG GATCGGGCCA TTGCCTGCCG TAACATCCAG
    GCCGTACAGG TGTTCCTGCG CAAGGGGGCC AAACTGGGAC CGACCACTTG GAGCATGGCC
    ATGGGCAAGC CGGAGATTCT GGTCATACTA CTGAACAAGC TGCTCGAGGA CGGTAATGTG
    CTGTACCGGA AAAACCGCTT CCAGGAGGCT GCTCATCGCT ATCAGTATGC CCTACGCAAG
    ATCTCCGGAC TGGAGCAGTT GCTCGAAAGG AATGCCATCT TCGCCCAGCT GCGGACCAAT
    CTTCTGCTGA ATCTGTCGCG CTGCAAGCGA AAACTGAATG AACTTGACGC CTCCATAGAT
    TTGGCCACGC AAGCGATTGC CCAGAAGCCG CACAGCTACG AAGGATACTA CGCCAGGGCA
    AAGGCGCGCA TGGAACTTGG TGCATTGAAT GAAGCACTTG TGGACGCCAA TGAGGCGATG
    CAGCAGGCCG CCCAAAGCGG TGTGCTCTGC GAAGTGGTCG AGGTGCTGAA GCGCATCCAG
    ACCGAGCTGC TCACGCGGAT CAGCATCAGC AGCGAGGATC AGACCGGCAG GATGTCGAAT
    ATCGGCGGCG GGGCATCCGT CGCATACGAA TCTCATCATG AGATCACCGA TTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa18306
    Clone ID Related Accession (Same CDS sequence) NM_001274808.1
    Accession Version NM_001274808.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5400bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product rolling pebbles, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCCCTCGC TGCAGCAAAT TGCGCTCGCG ATGGAAGGTG ACCAATCGGA GCTACAGACA 
    CCGCCGCTGC CGATCAACCC GCCGCCCAAG GTGATGGCCA ATGGAATCGT CTATGGCTTT
    CGCACCCGAC ACTTGTCCTG CATCGAGGAG AACGACTCGG ACTCGTTGCA CGGATCGTCC
    ATTCCACATG CCTACGATAT GATGCAGCAG GCGGACGAGA CCTTCCAGAA GTTGAATGCC
    AACGAGGAGC AGGACGAGTC GGGTAGCGAG GATGGGGGAT TGGAGAATCT GGACGATACG
    ATCGCAATGA TCGATGAATC GCGCCTTTTG GAGGCCGATT ATCTGGATGA GCTCGTGGCC
    GAGGAGCAGT TGCAGGAGCA GGACGAGGAT GAGGAGGAAT CCTCTGATCT TGTCGACTCT
    GGCAATGTGG AGGAGGATTT CTGGCAGCCG AGCTTCAATC GGAATGACAT CATGATGACC
    TCGGTTTATG GAGCTCTGAA TGGCTCGTTG ACGAGCGAAG TGTCTTCAAC TGCAGCGCCG
    GGCTACTCCG AGTGTGCCAC GCCCAGAAAG GAGGCGGAAC CCGTTTATGC CACGCCGGAA
    AAGCGACGAG CCAGCAATCG GAGTTTCGAC TCCACCTGCG CCTCGCTCAC CAGTTCCTTA
    TACGGCGGAT CTATGAACTC CTCGCTGGAT CTCAAGTACT CATCCTTGGG TGGTGGCGAC
    TCGGTCAGTG GTGGCATTAG TGGAACCAGC ACTTTGCGAG GAACTTCAGT GCCGCCAATG
    GATCTGTCCA TGATATCCAG CGATGGCGTT AGTGCGCTGG ACTGGGGATC TGCCAGTGTG
    AGCTGTGTTT TGGACTCCGG CGATGGGAAT CCCGCCGAGG AGCTCAACGA GGAAATGGCC
    GCCAAGTGCT TGAGTGCAGC GGAAAGTGAT CTCCAGTCCA TCCGACGACT CCTGGAGCAC
    GATGCCAGTG GATCCGTTTG CCCCTCCTGC CGCATATCCT TCGACAAGGG CAAGAGACGG
    AAACTGATCG ACACCTGCGG CCACGAGCGG TGCTATTCCT GCATGTTCCG CAACGACCAG
    TGCCCCATGT GCATGAACAG CTCGCTGAAA GATGTTGATG GCGCCAATGC TCAGGGCTAT
    GACACCGGAA TCGGTGGCTC CACATCGACG ATAGTGAGTC CTCTTGGATC CCCGCAGCCG
    CAGCTCAGAT CGCATGCGCA ACGAAACGCA GCGCTGGCGC GCTACATGCA GCAGCACCGC
    CAGGAGTCGC AGTCGCCAGA TTACCACCAT CGCTATGCGA GCAATGGCAA ACTGCCCCGG
    GCGGCGGCAC TGTCTACCAA TGGCAATGGA TTTACCACCG GCACCACGCA AACGGTCACA
    GTGGATGTGC ATCACCAGCT CGGCGGAGGA GGAGGAGGTG CTGCCGCCGC GGCCAAGCAG
    TTGATGGCCA ACGGCATCAG TGGCCATAGT CGCAGCAGCA GCATGAGCCA CAACATCCAT
    GCGGCAGCCT ACAGCGAACT GAGTGCCGCA CCACCGGCCT TCGCCACACC GCCGACGCGT
    AGGCGGTTCT TTAACCACAA GAATCTGCGC AGTGCCCTCA CTGGAGGAAG TGGCGGTGGA
    TCGGGAGTCG GCGGTGGTGT AGGAGGAGCT GGCGGTGTGG GCGGCGGTCA TCGACGCACT
    GCCTCCAATG GGGGTTGTCC CATTGACACA GCATCGATCC TGTCGGGAGG AAGCGACGCT
    ATTGACCACC ACTCCTCCTT CCTGAATGAG AAACTGCAGA AGGATCAGCT CCACGCCCGT
    CTCGGTCTGC TGCTCAACGA TCCCGGCAGC AATGGAAACA GCAGCAGCAG CGGAAGTGGT
    TGTGAACCCA TCTCGGCCCA TTCCACCACC AGCACCACGA GCAGCAGCGG CGTGGGAGCG
    GCCAGCACCA CCACGAGCGG TTCCTCGCAG AATGTCAGTC CGGAGCAAAC GCTGGCCAGC
    GGAGCCGGAA TGCTCAGTGG CAGTCAGCTG TCGGTGGCCA CCAGTCATGG GGTCAAGGAG
    GATGCACTGA GTCTGTGCGG CAAGTTCAAG GCTGGCTGCT CCATGCTGCA CGTCTACGAA
    GCATTGCCCA GCAAGAGTCG CAAAGGAAAT GTACGGAGAT CGACGCGTGG TCAGCAGGGA
    TCCAGTTCGA GCGCATCGGC GGCATCAGCA GTGGGATCCA GGGTGACAGC CTCCAGTTTG
    GCGGCTGTGC AGTTGGCCCT GAAGCCACTG TTCTTTGAGG TGCCGCTGCA GGAACCGGAT
    CCACCATATG TGGGTCGCCA GTGGCTGGTG CAGCAGTTGT CCAACATCCT GCTTGGCACC
    GAGACACGGG TGGTGCTCAT TAACGGACAG CCGGGCACTG GAAAGACGGC GTTTTGTCTG
    CAGCTGGTGG AGTACTCCTG CTTTGGCCGC CGGCAGATGC AGGATGATCC CGATGGCATA
    TACAGTCAGC TACAGTTGGG CGCCCACTGT GAGCGCATGC GCGGACTGGC CTCCCACATG
    GTTGGCTATC ACTTCTGCCA GGCGGACGCC AATCTCACCT GCCAGGTGCC CGATTTCGTG
    CATTCTCTGG CCGCCCAACT GTGTCAGGCG CCGCAGTTAA CCGCCTACAG GGATTACCTG
    CTCTCGGAGC CGCATCTTCA GGACATTCTC AGTGTGCGAG AGTGCATCGC GGATGCGGAG
    AGAGTAATGA AGCTGGCTAT ACTGGAACCA CTGGCTCACC TTCACCGGGC GGGAAAGATA
    CCCGCCAAAG TGGCCGTCAT CGTGGTGGAT GCCTTGTGCG AAGCGGAGTA CCATCGTCCC
    GATCATGGTC ACACCATTGC TAGTTTCTTG GCACAGCTTA CGCCACATTT TCCAGCTTGG
    CTCAAGCTGG TGGCCACTGT GAGGACCCAG ATGTTGGAGC TGGTGAAGGC ACCCAGCTAC
    ACACAACTTA CCCTGGACAG TTGGGCCAGC AGCCAGGCGT TGCAGCAGGA TATGCTTGAT
    TACATAGGCG CTCGGCTTGC AGATAGTCCG GAAATTCGAA TGAACATCGG CGGCGGAGGA
    GGCCAGAACT CGCAGTCGGG CAGCCAGCCG CAGACGAAGT TCGTGTCCCA TCTTCAGTCC
    CTGAGCAGGG GCTCCATGCT GTACGCTAAG CTCATCCTGG ATCTCATTGC ACGCGGCCAG
    TTGGTTATCA AGTCATCCAG CTACAAGGTG CTGCCGGTCT CTCTTGCTCA GATCTTTCTG
    CTGCACTTCA ACCTGCGCTT TCCCACCGCC CGTAGCTTTG AGCAGGCTGC TCCGATCCTC
    AATATATGCC TGGCTGCTCT GTATCCTCTG ACGCTGGACG AGATCTACTA CAGTATGGAG
    GCACTGAGTC ACGGACGGGA GGCACTAAGC TGGCCGGACT TCATGCAACG CTTTAAGCTG
    CTGGATGGAT TTTTAATCAA GAGGTTGGAC AACACGTACA TGTTCTTTCA TAGCTCCTTG
    AGGGAGTGGC TCATGCGAAG GGATGAGGGG GAGTCCAACA AGTTCCTCTG CGACGCCCGT
    TTGGGTCATG CTGGAATCGC TTTCCGATTA TCCCGACTGC AGGCTCCATT GTCACCTCAG
    CTCACATTGG AACTGGGTCA CCACATGCTG AAAGCACATT TATACGGAGG AACTAGTCTG
    ACGCTGCTTT CTCCGCGGGA TTTGCAATCT TATTGGTTGG CGGGAGCGGC GGATAACATT
    TCATCCTCGC TGGGCGCCCT GCGAAATGTC TACAGTCCGA ACCTAAAGGT TTCCCGACTA
    GTTCTCTTGG CAGGAGCTTC ACCGAATCAC CGCACGGATT ACATGGGTGG AGCTCCGATC
    CTTTGCATAG CTGCGCATGA GGGAATCCTT CCCATGGTTA GCCTGCTCCT AGAATTCGGT
    GCCGATGTCG GTCTGACCAA CAGCCAGGGC TGTACTCCAT TAATACTGGC CGCCATGCGT
    GGCCACTGCG ACGTAGTGCG TCCATTGGTA GCCGCCGGCA GCAGCCTCGG TCAGTTGGAC
    ATCACGCAGC GCTGTGCCCT AGTTCATGCC GCCCGCATGG GTCACCTCAG TGTGGTCAAG
    TACTTGCTCG CCTGCGACTG GTCACCACGA CCGCACTCCC AAGATGTCAC CAGATCGGTT
    GCCTTGCAAC AGGCGCTCAT TGGAGCTGCC GCACAGGCGC ACTGCAAGAT CCTTGAGGAT
    CTTCTGGATC TCAATGAGAC CGAGTTTGAC CTGGACGTGA ATGGCATGGA GCCAAGCAGT
    GGTGAACTGG CGCTAACCGC CGCCGCACGG CACGGATGCA TTGATGTTGT AGGAATCTTG
    CTCTCCCGTG GCGCTCAGAT CGATGCAAGA AATCGGCAGG GATACTCGGC GCTTTGGTTG
    GCTGTCAAGG AGGGTCACTG GAGTGTGGTG GAGCATCTGC TGCAACGTGG TGCCCTGTTG
    GATGAACCAT TGGGACAGAC ACGCAAGACG CCGCTAATGA TTGCGGCGGA GGAGGGACAC
    CTGGAGCTGG TCGATCTGCT GCTGGCGCGG GGAGCGCAAC GAGAAGCTCA GGATCACGAG
    GGATTCACTG CACTCAGCTG GGCCTGTTTA CGTGGCCACT TGGCGGCGGC CAAGACGCTC
    ATAGAACATG GCTGTAATCG CCACCACGAG GATCACAATG GACGCACAGC CCTGGATCTG
    GCAGCCTATC AGGGAGCCGC TAGCCTGGTG ATCTATATCC TGGAGCAGGG CGGCAATCTG
    GAACACATTG ATGTTCATGG AATGCGTCCC CTGGATCGGG CCATTGCCTG CCGTAACATC
    CAGGCCGTAC AGGTGTTCCT GCGCAAGGGG GCCAAACTGG GACCGACCAC TTGGAGCATG
    GCCATGGGCA AGCCGGAGAT TCTGGTCATA CTACTGAACA AGCTGCTCGA GGACGGTAAT
    GTGCTGTACC GGAAAAACCG CTTCCAGGAG GCTGCTCATC GCTATCAGTA TGCCCTACGC
    AAGATCTCCG GACTGGAGCA GTTGCTCGAA AGGAATGCCA TCTTCGCCCA GCTGCGGACC
    AATCTTCTGC TGAATCTGTC GCGCTGCAAG CGAAAACTGA ATGAACTTGA CGCCTCCATA
    GATTTGGCCA CGCAAGCGAT TGCCCAGAAG CCGCACAGCT ACGAAGGATA CTACGCCAGG
    GCAAAGGCGC GCATGGAACT TGGTGCATTG AATGAAGCAC TTGTGGACGC CAATGAGGCG
    ATGCAGCAGG CCGCCCAAAG CGGTGTGCTC TGCGAAGTGG TCGAGGTGCT GAAGCGCATC
    CAGACCGAGC TGCTCACGCG GATCAGCATC AGCAGCGAGG ATCAGACCGG CAGGATGTCG
    AATATCGGCG GCGGGGCATC CGTCGCATAC GAATCTCATC ATGAGATCAC CGATTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261737.1
    CDS300..5699
    Translation

    Target ORF information:

    RefSeq Version NM_001274808.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster rolling pebbles (rols), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001274808.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCCCTCGC TGCAGCAAAT TGCGCTCGCG ATGGAAGGTG ACCAATCGGA GCTACAGACA 
    CCGCCGCTGC CGATCAACCC GCCGCCCAAG GTGATGGCCA ATGGAATCGT CTATGGCTTT
    CGCACCCGAC ACTTGTCCTG CATCGAGGAG AACGACTCGG ACTCGTTGCA CGGATCGTCC
    ATTCCACATG CCTACGATAT GATGCAGCAG GCGGACGAGA CCTTCCAGAA GTTGAATGCC
    AACGAGGAGC AGGACGAGTC GGGTAGCGAG GATGGGGGAT TGGAGAATCT GGACGATACG
    ATCGCAATGA TCGATGAATC GCGCCTTTTG GAGGCCGATT ATCTGGATGA GCTCGTGGCC
    GAGGAGCAGT TGCAGGAGCA GGACGAGGAT GAGGAGGAAT CCTCTGATCT TGTCGACTCT
    GGCAATGTGG AGGAGGATTT CTGGCAGCCG AGCTTCAATC GGAATGACAT CATGATGACC
    TCGGTTTATG GAGCTCTGAA TGGCTCGTTG ACGAGCGAAG TGTCTTCAAC TGCAGCGCCG
    GGCTACTCCG AGTGTGCCAC GCCCAGAAAG GAGGCGGAAC CCGTTTATGC CACGCCGGAA
    AAGCGACGAG CCAGCAATCG GAGTTTCGAC TCCACCTGCG CCTCGCTCAC CAGTTCCTTA
    TACGGCGGAT CTATGAACTC CTCGCTGGAT CTCAAGTACT CATCCTTGGG TGGTGGCGAC
    TCGGTCAGTG GTGGCATTAG TGGAACCAGC ACTTTGCGAG GAACTTCAGT GCCGCCAATG
    GATCTGTCCA TGATATCCAG CGATGGCGTT AGTGCGCTGG ACTGGGGATC TGCCAGTGTG
    AGCTGTGTTT TGGACTCCGG CGATGGGAAT CCCGCCGAGG AGCTCAACGA GGAAATGGCC
    GCCAAGTGCT TGAGTGCAGC GGAAAGTGAT CTCCAGTCCA TCCGACGACT CCTGGAGCAC
    GATGCCAGTG GATCCGTTTG CCCCTCCTGC CGCATATCCT TCGACAAGGG CAAGAGACGG
    AAACTGATCG ACACCTGCGG CCACGAGCGG TGCTATTCCT GCATGTTCCG CAACGACCAG
    TGCCCCATGT GCATGAACAG CTCGCTGAAA GATGTTGATG GCGCCAATGC TCAGGGCTAT
    GACACCGGAA TCGGTGGCTC CACATCGACG ATAGTGAGTC CTCTTGGATC CCCGCAGCCG
    CAGCTCAGAT CGCATGCGCA ACGAAACGCA GCGCTGGCGC GCTACATGCA GCAGCACCGC
    CAGGAGTCGC AGTCGCCAGA TTACCACCAT CGCTATGCGA GCAATGGCAA ACTGCCCCGG
    GCGGCGGCAC TGTCTACCAA TGGCAATGGA TTTACCACCG GCACCACGCA AACGGTCACA
    GTGGATGTGC ATCACCAGCT CGGCGGAGGA GGAGGAGGTG CTGCCGCCGC GGCCAAGCAG
    TTGATGGCCA ACGGCATCAG TGGCCATAGT CGCAGCAGCA GCATGAGCCA CAACATCCAT
    GCGGCAGCCT ACAGCGAACT GAGTGCCGCA CCACCGGCCT TCGCCACACC GCCGACGCGT
    AGGCGGTTCT TTAACCACAA GAATCTGCGC AGTGCCCTCA CTGGAGGAAG TGGCGGTGGA
    TCGGGAGTCG GCGGTGGTGT AGGAGGAGCT GGCGGTGTGG GCGGCGGTCA TCGACGCACT
    GCCTCCAATG GGGGTTGTCC CATTGACACA GCATCGATCC TGTCGGGAGG AAGCGACGCT
    ATTGACCACC ACTCCTCCTT CCTGAATGAG AAACTGCAGA AGGATCAGCT CCACGCCCGT
    CTCGGTCTGC TGCTCAACGA TCCCGGCAGC AATGGAAACA GCAGCAGCAG CGGAAGTGGT
    TGTGAACCCA TCTCGGCCCA TTCCACCACC AGCACCACGA GCAGCAGCGG CGTGGGAGCG
    GCCAGCACCA CCACGAGCGG TTCCTCGCAG AATGTCAGTC CGGAGCAAAC GCTGGCCAGC
    GGAGCCGGAA TGCTCAGTGG CAGTCAGCTG TCGGTGGCCA CCAGTCATGG GGTCAAGGAG
    GATGCACTGA GTCTGTGCGG CAAGTTCAAG GCTGGCTGCT CCATGCTGCA CGTCTACGAA
    GCATTGCCCA GCAAGAGTCG CAAAGGAAAT GTACGGAGAT CGACGCGTGG TCAGCAGGGA
    TCCAGTTCGA GCGCATCGGC GGCATCAGCA GTGGGATCCA GGGTGACAGC CTCCAGTTTG
    GCGGCTGTGC AGTTGGCCCT GAAGCCACTG TTCTTTGAGG TGCCGCTGCA GGAACCGGAT
    CCACCATATG TGGGTCGCCA GTGGCTGGTG CAGCAGTTGT CCAACATCCT GCTTGGCACC
    GAGACACGGG TGGTGCTCAT TAACGGACAG CCGGGCACTG GAAAGACGGC GTTTTGTCTG
    CAGCTGGTGG AGTACTCCTG CTTTGGCCGC CGGCAGATGC AGGATGATCC CGATGGCATA
    TACAGTCAGC TACAGTTGGG CGCCCACTGT GAGCGCATGC GCGGACTGGC CTCCCACATG
    GTTGGCTATC ACTTCTGCCA GGCGGACGCC AATCTCACCT GCCAGGTGCC CGATTTCGTG
    CATTCTCTGG CCGCCCAACT GTGTCAGGCG CCGCAGTTAA CCGCCTACAG GGATTACCTG
    CTCTCGGAGC CGCATCTTCA GGACATTCTC AGTGTGCGAG AGTGCATCGC GGATGCGGAG
    AGAGTAATGA AGCTGGCTAT ACTGGAACCA CTGGCTCACC TTCACCGGGC GGGAAAGATA
    CCCGCCAAAG TGGCCGTCAT CGTGGTGGAT GCCTTGTGCG AAGCGGAGTA CCATCGTCCC
    GATCATGGTC ACACCATTGC TAGTTTCTTG GCACAGCTTA CGCCACATTT TCCAGCTTGG
    CTCAAGCTGG TGGCCACTGT GAGGACCCAG ATGTTGGAGC TGGTGAAGGC ACCCAGCTAC
    ACACAACTTA CCCTGGACAG TTGGGCCAGC AGCCAGGCGT TGCAGCAGGA TATGCTTGAT
    TACATAGGCG CTCGGCTTGC AGATAGTCCG GAAATTCGAA TGAACATCGG CGGCGGAGGA
    GGCCAGAACT CGCAGTCGGG CAGCCAGCCG CAGACGAAGT TCGTGTCCCA TCTTCAGTCC
    CTGAGCAGGG GCTCCATGCT GTACGCTAAG CTCATCCTGG ATCTCATTGC ACGCGGCCAG
    TTGGTTATCA AGTCATCCAG CTACAAGGTG CTGCCGGTCT CTCTTGCTCA GATCTTTCTG
    CTGCACTTCA ACCTGCGCTT TCCCACCGCC CGTAGCTTTG AGCAGGCTGC TCCGATCCTC
    AATATATGCC TGGCTGCTCT GTATCCTCTG ACGCTGGACG AGATCTACTA CAGTATGGAG
    GCACTGAGTC ACGGACGGGA GGCACTAAGC TGGCCGGACT TCATGCAACG CTTTAAGCTG
    CTGGATGGAT TTTTAATCAA GAGGTTGGAC AACACGTACA TGTTCTTTCA TAGCTCCTTG
    AGGGAGTGGC TCATGCGAAG GGATGAGGGG GAGTCCAACA AGTTCCTCTG CGACGCCCGT
    TTGGGTCATG CTGGAATCGC TTTCCGATTA TCCCGACTGC AGGCTCCATT GTCACCTCAG
    CTCACATTGG AACTGGGTCA CCACATGCTG AAAGCACATT TATACGGAGG AACTAGTCTG
    ACGCTGCTTT CTCCGCGGGA TTTGCAATCT TATTGGTTGG CGGGAGCGGC GGATAACATT
    TCATCCTCGC TGGGCGCCCT GCGAAATGTC TACAGTCCGA ACCTAAAGGT TTCCCGACTA
    GTTCTCTTGG CAGGAGCTTC ACCGAATCAC CGCACGGATT ACATGGGTGG AGCTCCGATC
    CTTTGCATAG CTGCGCATGA GGGAATCCTT CCCATGGTTA GCCTGCTCCT AGAATTCGGT
    GCCGATGTCG GTCTGACCAA CAGCCAGGGC TGTACTCCAT TAATACTGGC CGCCATGCGT
    GGCCACTGCG ACGTAGTGCG TCCATTGGTA GCCGCCGGCA GCAGCCTCGG TCAGTTGGAC
    ATCACGCAGC GCTGTGCCCT AGTTCATGCC GCCCGCATGG GTCACCTCAG TGTGGTCAAG
    TACTTGCTCG CCTGCGACTG GTCACCACGA CCGCACTCCC AAGATGTCAC CAGATCGGTT
    GCCTTGCAAC AGGCGCTCAT TGGAGCTGCC GCACAGGCGC ACTGCAAGAT CCTTGAGGAT
    CTTCTGGATC TCAATGAGAC CGAGTTTGAC CTGGACGTGA ATGGCATGGA GCCAAGCAGT
    GGTGAACTGG CGCTAACCGC CGCCGCACGG CACGGATGCA TTGATGTTGT AGGAATCTTG
    CTCTCCCGTG GCGCTCAGAT CGATGCAAGA AATCGGCAGG GATACTCGGC GCTTTGGTTG
    GCTGTCAAGG AGGGTCACTG GAGTGTGGTG GAGCATCTGC TGCAACGTGG TGCCCTGTTG
    GATGAACCAT TGGGACAGAC ACGCAAGACG CCGCTAATGA TTGCGGCGGA GGAGGGACAC
    CTGGAGCTGG TCGATCTGCT GCTGGCGCGG GGAGCGCAAC GAGAAGCTCA GGATCACGAG
    GGATTCACTG CACTCAGCTG GGCCTGTTTA CGTGGCCACT TGGCGGCGGC CAAGACGCTC
    ATAGAACATG GCTGTAATCG CCACCACGAG GATCACAATG GACGCACAGC CCTGGATCTG
    GCAGCCTATC AGGGAGCCGC TAGCCTGGTG ATCTATATCC TGGAGCAGGG CGGCAATCTG
    GAACACATTG ATGTTCATGG AATGCGTCCC CTGGATCGGG CCATTGCCTG CCGTAACATC
    CAGGCCGTAC AGGTGTTCCT GCGCAAGGGG GCCAAACTGG GACCGACCAC TTGGAGCATG
    GCCATGGGCA AGCCGGAGAT TCTGGTCATA CTACTGAACA AGCTGCTCGA GGACGGTAAT
    GTGCTGTACC GGAAAAACCG CTTCCAGGAG GCTGCTCATC GCTATCAGTA TGCCCTACGC
    AAGATCTCCG GACTGGAGCA GTTGCTCGAA AGGAATGCCA TCTTCGCCCA GCTGCGGACC
    AATCTTCTGC TGAATCTGTC GCGCTGCAAG CGAAAACTGA ATGAACTTGA CGCCTCCATA
    GATTTGGCCA CGCAAGCGAT TGCCCAGAAG CCGCACAGCT ACGAAGGATA CTACGCCAGG
    GCAAAGGCGC GCATGGAACT TGGTGCATTG AATGAAGCAC TTGTGGACGC CAATGAGGCG
    ATGCAGCAGG CCGCCCAAAG CGGTGTGCTC TGCGAAGTGG TCGAGGTGCT GAAGCGCATC
    CAGACCGAGC TGCTCACGCG GATCAGCATC AGCAGCGAGG ATCAGACCGG CAGGATGTCG
    AATATCGGCG GCGGGGCATC CGTCGCATAC GAATCTCATC ATGAGATCAC CGATTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa19983
    Clone ID Related Accession (Same CDS sequence) NM_168487.2
    Accession Version NM_168487.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5703bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product rolling pebbles, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168487.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGCCCTCGC TGCAGCAAAT TGCGCTCGCG ATGGAAGGTG ACCAATCGGA GCTACAGACA 
    CCGCCGCTGC CGATCAACCC GCCGCCCAAG GTGATGGCCA ATGGAATCGT CTATGGCTTT
    CGCACCCGAC ACTTGTCCTG CATCGAGGAG AACGACTCGG ACTCGTTGCA CGGATCGTCC
    ATTCCACATG CCTACGATAT GATGCAGCAG GCGGACGAGA CCTTCCAGAA GTTGAATGCC
    AACGAGGAGC AGGACGAGTC GGGTAGCGAG GATGGGGGAT TGGAGAATCT GGACGATACG
    ATCGCAATGA TCGATGAATC GCGCCTTTTG GAGGCCGATT ATCTGGATGA GCTCGTGGCC
    GAGGAGCAGT TGCAGGAGCA GGACGAGGAT GAGGAGGAAT CCTCTGATCT TGTCGACTCT
    GGCAATGTGG AGGAGGATTT CTGGCAGCCG AGCTTCAATC GGAATGACAT CATGATGACC
    TCGGTTTATG GAGCTCTGAA TGGCTCGTTG ACGAGCGAAG TGTCTTCAAC TGCAGCGCCG
    GGCTACTCCG AGTGTGCCAC GCCCAGAAAG GAGGCGGAAC CCGTTTATGC CACGCCGGAA
    AAGCGACGAG CCAGCAATCG GAGTTTCGAC TCCACCTGCG CCTCGCTCAC CAGTTCCTTA
    TACGGCGGAT CTATGAACTC CTCGCTGGAT CTCAAGTACT CATCCTTGGG TGGTGGCGAC
    TCGGTCAGTG GTGGCATTAG TGGAACCAGC ACTTTGCGAG GAACTTCAGT GCCGCCAATG
    GATCTGTCCA TGATATCCAG CGATGGCGTT AGTGCGCTGG ACTGGGGATC TGCCAGTGTG
    AGCTGTGTTT TGGACTCCGG CGATGGGAAT CCCGCCGAGG AGCTCAACGA GGAAATGGCC
    GCCAAGTGCT TGAGTGCAGC GGAAAGTGAT CTCCAGTCCA TCCGACGACT CCTGGAGCAC
    GATGCCAGTG GATCCGTTTG CCCCTCCTGC CGCATATCCT TCGACAAGGG CAAGAGACGG
    AAACTGATCG ACACCTGCGG CCACGAGCGG TGCTATTCCT GCATGTTCCG CAACGACCAG
    TGCCCCATGT GCATGAACAG CTCGCTGAAA GATGTTGATG GCGCCAATGC TCAGGGCTAT
    GACACCGGAA TCGGTGGCTC CACATCGACG ATAGTGAGTC CTCTTGGATC CCCGCAGCCG
    CAGCTCAGAT CGCATGCGCA ACGAAACGCA GCGCTGGCGC GCTACATGCA GCAGCACCGC
    CAGGAGTCGC AGTCGCCAGA TTACCACCAT CGCTATGCGA GCAATGGCAA ACTGCCCCGG
    GCGGCGGCAC TGTCTACCAA TGGCAATGGA TTTACCACCG GCACCACGCA AACGGTCACA
    GTGGATGTGC ATCACCAGCT CGGCGGAGGA GGAGGAGGTG CTGCCGCCGC GGCCAAGCAG
    TTGATGGCCA ACGGCATCAG TGGCCATAGT CGCAGCAGCA GCATGAGCCA CAACATCCAT
    GCGGCAGCCT ACAGCGAACT GAGTGCCGCA CCACCGGCCT TCGCCACACC GCCGACGCGT
    AGGCGGTTCT TTAACCACAA GAATCTGCGC AGTGCCCTCA CTGGAGGAAG TGGCGGTGGA
    TCGGGAGTCG GCGGTGGTGT AGGAGGAGCT GGCGGTGTGG GCGGCGGTCA TCGACGCACT
    GCCTCCAATG GGGGTTGTCC CATTGACACA GCATCGATCC TGTCGGGAGA TCATACTCAT
    CAGCATCATC ACCGCGACAA TCAGCCGGAG GGCAAGGAGT CCAATGCCCT TAACACATCC
    ACCTGCTCGG ACTCGGCGGT CACTAGGAGG CGGAGGAAGA ACGTGAGCAA TCACAACCTG
    AAGACCTCTG CCCGCCATGG AGCAAGTAGC GAGAATCGTC TGAACAGGCT CAGTTTGGCC
    GGAACGTCCG TCTATGCGGG TCATCTGTCC TCTTTGGTCT TTGGCAAGAT CAAGTCCCTG
    TGGAGCGTGA ACTCCTCCAA TTCCAGCGAG GCGGGACTGA ATCAATTAGC AGGAAGCGAC
    GCTATTGACC ACCACTCCTC CTTCCTGAAT GAGAAACTGC AGAAGGATCA GCTCCACGCC
    CGTCTCGGTC TGCTGCTCAA CGATCCCGGC AGCAATGGAA ACAGCAGCAG CAGCGGAAGT
    GGTTGTGAAC CCATCTCGGC CCATTCCACC ACCAGCACCA CGAGCAGCAG CGGCGTGGGA
    GCGGCCAGCA CCACCACGAG CGGTTCCTCG CAGAATGTCA GTCCGGAGCA AACGCTGGCC
    AGCGGAGCCG GAATGCTCAG TGGCAGTCAG CTGTCGGTGG CCACCAGTCA TGGGGTCAAG
    GAGGATGCAC TGAGTCTGTG CGGCAAGTTC AAGGCTGGCT GCTCCATGCT GCACGTCTAC
    GAAGCATTGC CCAGCAAGAG TCGCAAAGGA AATGTACGGA GATCGACGCG TGGTCAGCAG
    GGATCCAGTT CGAGCGCATC GGCGGCATCA GCAGTGGGAT CCAGGGTGAC AGCCTCCAGT
    TTGGCGGCTG TGCAGTTGGC CCTGAAGCCA CTGTTCTTTG AGGTGCCGCT GCAGGAACCG
    GATCCACCAT ATGTGGGTCG CCAGTGGCTG GTGCAGCAGT TGTCCAACAT CCTGCTTGGC
    ACCGAGACAC GGGTGGTGCT CATTAACGGA CAGCCGGGCA CTGGAAAGAC GGCGTTTTGT
    CTGCAGCTGG TGGAGTACTC CTGCTTTGGC CGCCGGCAGA TGCAGGATGA TCCCGATGGC
    ATATACAGTC AGCTACAGTT GGGCGCCCAC TGTGAGCGCA TGCGCGGACT GGCCTCCCAC
    ATGGTTGGCT ATCACTTCTG CCAGGCGGAC GCCAATCTCA CCTGCCAGGT GCCCGATTTC
    GTGCATTCTC TGGCCGCCCA ACTGTGTCAG GCGCCGCAGT TAACCGCCTA CAGGGATTAC
    CTGCTCTCGG AGCCGCATCT TCAGGACATT CTCAGTGTGC GAGAGTGCAT CGCGGATGCG
    GAGAGAGTAA TGAAGCTGGC TATACTGGAA CCACTGGCTC ACCTTCACCG GGCGGGAAAG
    ATACCCGCCA AAGTGGCCGT CATCGTGGTG GATGCCTTGT GCGAAGCGGA GTACCATCGT
    CCCGATCATG GTCACACCAT TGCTAGTTTC TTGGCACAGC TTACGCCACA TTTTCCAGCT
    TGGCTCAAGC TGGTGGCCAC TGTGAGGACC CAGATGTTGG AGCTGGTGAA GGCACCCAGC
    TACACACAAC TTACCCTGGA CAGTTGGGCC AGCAGCCAGG CGTTGCAGCA GGATATGCTT
    GATTACATAG GCGCTCGGCT TGCAGATAGT CCGGAAATTC GAATGAACAT CGGCGGCGGA
    GGAGGCCAGA ACTCGCAGTC GGGCAGCCAG CCGCAGACGA AGTTCGTGTC CCATCTTCAG
    TCCCTGAGCA GGGGCTCCAT GCTGTACGCT AAGCTCATCC TGGATCTCAT TGCACGCGGC
    CAGTTGGTTA TCAAGTCATC CAGCTACAAG GTGCTGCCGG TCTCTCTTGC TCAGATCTTT
    CTGCTGCACT TCAACCTGCG CTTTCCCACC GCCCGTAGCT TTGAGCAGGC TGCTCCGATC
    CTCAATATAT GCCTGGCTGC TCTGTATCCT CTGACGCTGG ACGAGATCTA CTACAGTATG
    GAGGCACTGA GTCACGGACG GGAGGCACTA AGCTGGCCGG ACTTCATGCA ACGCTTTAAG
    CTGCTGGATG GATTTTTAAT CAAGAGGTTG GACAACACGT ACATGTTCTT TCATAGCTCC
    TTGAGGGAGT GGCTCATGCG AAGGGATGAG GGGGAGTCCA ACAAGTTCCT CTGCGACGCC
    CGTTTGGGTC ATGCTGGAAT CGCTTTCCGA TTATCCCGAC TGCAGGCTCC ATTGTCACCT
    CAGCTCACAT TGGAACTGGG TCACCACATG CTGAAAGCAC ATTTATACGG AGGAACTAGT
    CTGACGCTGC TTTCTCCGCG GGATTTGCAA TCTTATTGGT TGGCGGGAGC GGCGGATAAC
    ATTTCATCCT CGCTGGGCGC CCTGCGAAAT GTCTACAGTC CGAACCTAAA GGTTTCCCGA
    CTAGTTCTCT TGGCAGGAGC TTCACCGAAT CACCGCACGG ATTACATGGG TGGAGCTCCG
    ATCCTTTGCA TAGCTGCGCA TGAGGGAATC CTTCCCATGG TTAGCCTGCT CCTAGAATTC
    GGTGCCGATG TCGGTCTGAC CAACAGCCAG GGCTGTACTC CATTAATACT GGCCGCCATG
    CGTGGCCACT GCGACGTAGT GCGTCCATTG GTAGCCGCCG GCAGCAGCCT CGGTCAGTTG
    GACATCACGC AGCGCTGTGC CCTAGTTCAT GCCGCCCGCA TGGGTCACCT CAGTGTGGTC
    AAGTACTTGC TCGCCTGCGA CTGGTCACCA CGACCGCACT CCCAAGATGT CACCAGATCG
    GTTGCCTTGC AACAGGCGCT CATTGGAGCT GCCGCACAGG CGCACTGCAA GATCCTTGAG
    GATCTTCTGG ATCTCAATGA GACCGAGTTT GACCTGGACG TGAATGGCAT GGAGCCAAGC
    AGTGGTGAAC TGGCGCTAAC CGCCGCCGCA CGGCACGGAT GCATTGATGT TGTAGGAATC
    TTGCTCTCCC GTGGCGCTCA GATCGATGCA AGAAATCGGC AGGGATACTC GGCGCTTTGG
    TTGGCTGTCA AGGAGGGTCA CTGGAGTGTG GTGGAGCATC TGCTGCAACG TGGTGCCCTG
    TTGGATGAAC CATTGGGACA GACACGCAAG ACGCCGCTAA TGATTGCGGC GGAGGAGGGA
    CACCTGGAGC TGGTCGATCT GCTGCTGGCG CGGGGAGCGC AACGAGAAGC TCAGGATCAC
    GAGGGATTCA CTGCACTCAG CTGGGCCTGT TTACGTGGCC ACTTGGCGGC GGCCAAGACG
    CTCATAGAAC ATGGCTGTAA TCGCCACCAC GAGGATCACA ATGGACGCAC AGCCCTGGAT
    CTGGCAGCCT ATCAGGGAGC CGCTAGCCTG GTGATCTATA TCCTGGAGCA GGGCGGCAAT
    CTGGAACACA TTGATGTTCA TGGAATGCGT CCCCTGGATC GGGCCATTGC CTGCCGTAAC
    ATCCAGGCCG TACAGGTGTT CCTGCGCAAG GGGGCCAAAC TGGGACCGAC CACTTGGAGC
    ATGGCCATGG GCAAGCCGGA GATTCTGGTC ATACTACTGA ACAAGCTGCT CGAGGACGGT
    AATGTGCTGT ACCGGAAAAA CCGCTTCCAG GAGGCTGCTC ATCGCTATCA GTATGCCCTA
    CGCAAGATCT CCGGACTGGA GCAGTTGCTC GAAAGGAATG CCATCTTCGC CCAGCTGCGG
    ACCAATCTTC TGCTGAATCT GTCGCGCTGC AAGCGAAAAC TGAATGAACT TGACGCCTCC
    ATAGATTTGG CCACGCAAGC GATTGCCCAG AAGCCGCACA GCTACGAAGG ATACTACGCC
    AGGGCAAAGG CGCGCATGGA ACTTGGTGCA TTGAATGAAG CACTTGTGGA CGCCAATGAG
    GCGATGCAGC AGGCCGCCCA AAGCGGTGTG CTCTGCGAAG TGGTCGAGGT GCTGAAGCGC
    ATCCAGACCG AGCTGCTCAC GCGGATCAGC ATCAGCAGCG AGGATCAGAC CGGCAGGATG
    TCGAATATCG GCGGCGGGGC ATCCGTCGCA TACGAATCTC ATCATGAGAT CACCGATTTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_729778.1
    CDS300..6002
    Translation

    Target ORF information:

    RefSeq Version NM_168487.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster rolling pebbles (rols), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_168487.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGCCCTCGC TGCAGCAAAT TGCGCTCGCG ATGGAAGGTG ACCAATCGGA GCTACAGACA 
    CCGCCGCTGC CGATCAACCC GCCGCCCAAG GTGATGGCCA ATGGAATCGT CTATGGCTTT
    CGCACCCGAC ACTTGTCCTG CATCGAGGAG AACGACTCGG ACTCGTTGCA CGGATCGTCC
    ATTCCACATG CCTACGATAT GATGCAGCAG GCGGACGAGA CCTTCCAGAA GTTGAATGCC
    AACGAGGAGC AGGACGAGTC GGGTAGCGAG GATGGGGGAT TGGAGAATCT GGACGATACG
    ATCGCAATGA TCGATGAATC GCGCCTTTTG GAGGCCGATT ATCTGGATGA GCTCGTGGCC
    GAGGAGCAGT TGCAGGAGCA GGACGAGGAT GAGGAGGAAT CCTCTGATCT TGTCGACTCT
    GGCAATGTGG AGGAGGATTT CTGGCAGCCG AGCTTCAATC GGAATGACAT CATGATGACC
    TCGGTTTATG GAGCTCTGAA TGGCTCGTTG ACGAGCGAAG TGTCTTCAAC TGCAGCGCCG
    GGCTACTCCG AGTGTGCCAC GCCCAGAAAG GAGGCGGAAC CCGTTTATGC CACGCCGGAA
    AAGCGACGAG CCAGCAATCG GAGTTTCGAC TCCACCTGCG CCTCGCTCAC CAGTTCCTTA
    TACGGCGGAT CTATGAACTC CTCGCTGGAT CTCAAGTACT CATCCTTGGG TGGTGGCGAC
    TCGGTCAGTG GTGGCATTAG TGGAACCAGC ACTTTGCGAG GAACTTCAGT GCCGCCAATG
    GATCTGTCCA TGATATCCAG CGATGGCGTT AGTGCGCTGG ACTGGGGATC TGCCAGTGTG
    AGCTGTGTTT TGGACTCCGG CGATGGGAAT CCCGCCGAGG AGCTCAACGA GGAAATGGCC
    GCCAAGTGCT TGAGTGCAGC GGAAAGTGAT CTCCAGTCCA TCCGACGACT CCTGGAGCAC
    GATGCCAGTG GATCCGTTTG CCCCTCCTGC CGCATATCCT TCGACAAGGG CAAGAGACGG
    AAACTGATCG ACACCTGCGG CCACGAGCGG TGCTATTCCT GCATGTTCCG CAACGACCAG
    TGCCCCATGT GCATGAACAG CTCGCTGAAA GATGTTGATG GCGCCAATGC TCAGGGCTAT
    GACACCGGAA TCGGTGGCTC CACATCGACG ATAGTGAGTC CTCTTGGATC CCCGCAGCCG
    CAGCTCAGAT CGCATGCGCA ACGAAACGCA GCGCTGGCGC GCTACATGCA GCAGCACCGC
    CAGGAGTCGC AGTCGCCAGA TTACCACCAT CGCTATGCGA GCAATGGCAA ACTGCCCCGG
    GCGGCGGCAC TGTCTACCAA TGGCAATGGA TTTACCACCG GCACCACGCA AACGGTCACA
    GTGGATGTGC ATCACCAGCT CGGCGGAGGA GGAGGAGGTG CTGCCGCCGC GGCCAAGCAG
    TTGATGGCCA ACGGCATCAG TGGCCATAGT CGCAGCAGCA GCATGAGCCA CAACATCCAT
    GCGGCAGCCT ACAGCGAACT GAGTGCCGCA CCACCGGCCT TCGCCACACC GCCGACGCGT
    AGGCGGTTCT TTAACCACAA GAATCTGCGC AGTGCCCTCA CTGGAGGAAG TGGCGGTGGA
    TCGGGAGTCG GCGGTGGTGT AGGAGGAGCT GGCGGTGTGG GCGGCGGTCA TCGACGCACT
    GCCTCCAATG GGGGTTGTCC CATTGACACA GCATCGATCC TGTCGGGAGA TCATACTCAT
    CAGCATCATC ACCGCGACAA TCAGCCGGAG GGCAAGGAGT CCAATGCCCT TAACACATCC
    ACCTGCTCGG ACTCGGCGGT CACTAGGAGG CGGAGGAAGA ACGTGAGCAA TCACAACCTG
    AAGACCTCTG CCCGCCATGG AGCAAGTAGC GAGAATCGTC TGAACAGGCT CAGTTTGGCC
    GGAACGTCCG TCTATGCGGG TCATCTGTCC TCTTTGGTCT TTGGCAAGAT CAAGTCCCTG
    TGGAGCGTGA ACTCCTCCAA TTCCAGCGAG GCGGGACTGA ATCAATTAGC AGGAAGCGAC
    GCTATTGACC ACCACTCCTC CTTCCTGAAT GAGAAACTGC AGAAGGATCA GCTCCACGCC
    CGTCTCGGTC TGCTGCTCAA CGATCCCGGC AGCAATGGAA ACAGCAGCAG CAGCGGAAGT
    GGTTGTGAAC CCATCTCGGC CCATTCCACC ACCAGCACCA CGAGCAGCAG CGGCGTGGGA
    GCGGCCAGCA CCACCACGAG CGGTTCCTCG CAGAATGTCA GTCCGGAGCA AACGCTGGCC
    AGCGGAGCCG GAATGCTCAG TGGCAGTCAG CTGTCGGTGG CCACCAGTCA TGGGGTCAAG
    GAGGATGCAC TGAGTCTGTG CGGCAAGTTC AAGGCTGGCT GCTCCATGCT GCACGTCTAC
    GAAGCATTGC CCAGCAAGAG TCGCAAAGGA AATGTACGGA GATCGACGCG TGGTCAGCAG
    GGATCCAGTT CGAGCGCATC GGCGGCATCA GCAGTGGGAT CCAGGGTGAC AGCCTCCAGT
    TTGGCGGCTG TGCAGTTGGC CCTGAAGCCA CTGTTCTTTG AGGTGCCGCT GCAGGAACCG
    GATCCACCAT ATGTGGGTCG CCAGTGGCTG GTGCAGCAGT TGTCCAACAT CCTGCTTGGC
    ACCGAGACAC GGGTGGTGCT CATTAACGGA CAGCCGGGCA CTGGAAAGAC GGCGTTTTGT
    CTGCAGCTGG TGGAGTACTC CTGCTTTGGC CGCCGGCAGA TGCAGGATGA TCCCGATGGC
    ATATACAGTC AGCTACAGTT GGGCGCCCAC TGTGAGCGCA TGCGCGGACT GGCCTCCCAC
    ATGGTTGGCT ATCACTTCTG CCAGGCGGAC GCCAATCTCA CCTGCCAGGT GCCCGATTTC
    GTGCATTCTC TGGCCGCCCA ACTGTGTCAG GCGCCGCAGT TAACCGCCTA CAGGGATTAC
    CTGCTCTCGG AGCCGCATCT TCAGGACATT CTCAGTGTGC GAGAGTGCAT CGCGGATGCG
    GAGAGAGTAA TGAAGCTGGC TATACTGGAA CCACTGGCTC ACCTTCACCG GGCGGGAAAG
    ATACCCGCCA AAGTGGCCGT CATCGTGGTG GATGCCTTGT GCGAAGCGGA GTACCATCGT
    CCCGATCATG GTCACACCAT TGCTAGTTTC TTGGCACAGC TTACGCCACA TTTTCCAGCT
    TGGCTCAAGC TGGTGGCCAC TGTGAGGACC CAGATGTTGG AGCTGGTGAA GGCACCCAGC
    TACACACAAC TTACCCTGGA CAGTTGGGCC AGCAGCCAGG CGTTGCAGCA GGATATGCTT
    GATTACATAG GCGCTCGGCT TGCAGATAGT CCGGAAATTC GAATGAACAT CGGCGGCGGA
    GGAGGCCAGA ACTCGCAGTC GGGCAGCCAG CCGCAGACGA AGTTCGTGTC CCATCTTCAG
    TCCCTGAGCA GGGGCTCCAT GCTGTACGCT AAGCTCATCC TGGATCTCAT TGCACGCGGC
    CAGTTGGTTA TCAAGTCATC CAGCTACAAG GTGCTGCCGG TCTCTCTTGC TCAGATCTTT
    CTGCTGCACT TCAACCTGCG CTTTCCCACC GCCCGTAGCT TTGAGCAGGC TGCTCCGATC
    CTCAATATAT GCCTGGCTGC TCTGTATCCT CTGACGCTGG ACGAGATCTA CTACAGTATG
    GAGGCACTGA GTCACGGACG GGAGGCACTA AGCTGGCCGG ACTTCATGCA ACGCTTTAAG
    CTGCTGGATG GATTTTTAAT CAAGAGGTTG GACAACACGT ACATGTTCTT TCATAGCTCC
    TTGAGGGAGT GGCTCATGCG AAGGGATGAG GGGGAGTCCA ACAAGTTCCT CTGCGACGCC
    CGTTTGGGTC ATGCTGGAAT CGCTTTCCGA TTATCCCGAC TGCAGGCTCC ATTGTCACCT
    CAGCTCACAT TGGAACTGGG TCACCACATG CTGAAAGCAC ATTTATACGG AGGAACTAGT
    CTGACGCTGC TTTCTCCGCG GGATTTGCAA TCTTATTGGT TGGCGGGAGC GGCGGATAAC
    ATTTCATCCT CGCTGGGCGC CCTGCGAAAT GTCTACAGTC CGAACCTAAA GGTTTCCCGA
    CTAGTTCTCT TGGCAGGAGC TTCACCGAAT CACCGCACGG ATTACATGGG TGGAGCTCCG
    ATCCTTTGCA TAGCTGCGCA TGAGGGAATC CTTCCCATGG TTAGCCTGCT CCTAGAATTC
    GGTGCCGATG TCGGTCTGAC CAACAGCCAG GGCTGTACTC CATTAATACT GGCCGCCATG
    CGTGGCCACT GCGACGTAGT GCGTCCATTG GTAGCCGCCG GCAGCAGCCT CGGTCAGTTG
    GACATCACGC AGCGCTGTGC CCTAGTTCAT GCCGCCCGCA TGGGTCACCT CAGTGTGGTC
    AAGTACTTGC TCGCCTGCGA CTGGTCACCA CGACCGCACT CCCAAGATGT CACCAGATCG
    GTTGCCTTGC AACAGGCGCT CATTGGAGCT GCCGCACAGG CGCACTGCAA GATCCTTGAG
    GATCTTCTGG ATCTCAATGA GACCGAGTTT GACCTGGACG TGAATGGCAT GGAGCCAAGC
    AGTGGTGAAC TGGCGCTAAC CGCCGCCGCA CGGCACGGAT GCATTGATGT TGTAGGAATC
    TTGCTCTCCC GTGGCGCTCA GATCGATGCA AGAAATCGGC AGGGATACTC GGCGCTTTGG
    TTGGCTGTCA AGGAGGGTCA CTGGAGTGTG GTGGAGCATC TGCTGCAACG TGGTGCCCTG
    TTGGATGAAC CATTGGGACA GACACGCAAG ACGCCGCTAA TGATTGCGGC GGAGGAGGGA
    CACCTGGAGC TGGTCGATCT GCTGCTGGCG CGGGGAGCGC AACGAGAAGC TCAGGATCAC
    GAGGGATTCA CTGCACTCAG CTGGGCCTGT TTACGTGGCC ACTTGGCGGC GGCCAAGACG
    CTCATAGAAC ATGGCTGTAA TCGCCACCAC GAGGATCACA ATGGACGCAC AGCCCTGGAT
    CTGGCAGCCT ATCAGGGAGC CGCTAGCCTG GTGATCTATA TCCTGGAGCA GGGCGGCAAT
    CTGGAACACA TTGATGTTCA TGGAATGCGT CCCCTGGATC GGGCCATTGC CTGCCGTAAC
    ATCCAGGCCG TACAGGTGTT CCTGCGCAAG GGGGCCAAAC TGGGACCGAC CACTTGGAGC
    ATGGCCATGG GCAAGCCGGA GATTCTGGTC ATACTACTGA ACAAGCTGCT CGAGGACGGT
    AATGTGCTGT ACCGGAAAAA CCGCTTCCAG GAGGCTGCTC ATCGCTATCA GTATGCCCTA
    CGCAAGATCT CCGGACTGGA GCAGTTGCTC GAAAGGAATG CCATCTTCGC CCAGCTGCGG
    ACCAATCTTC TGCTGAATCT GTCGCGCTGC AAGCGAAAAC TGAATGAACT TGACGCCTCC
    ATAGATTTGG CCACGCAAGC GATTGCCCAG AAGCCGCACA GCTACGAAGG ATACTACGCC
    AGGGCAAAGG CGCGCATGGA ACTTGGTGCA TTGAATGAAG CACTTGTGGA CGCCAATGAG
    GCGATGCAGC AGGCCGCCCA AAGCGGTGTG CTCTGCGAAG TGGTCGAGGT GCTGAAGCGC
    ATCCAGACCG AGCTGCTCAC GCGGATCAGC ATCAGCAGCG AGGATCAGAC CGGCAGGATG
    TCGAATATCG GCGGCGGGGC ATCCGTCGCA TACGAATCTC ATCATGAGAT CACCGATTTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa18307
    Clone ID Related Accession (Same CDS sequence) NM_001274809.1
    Accession Version NM_001274809.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product rolling pebbles, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTTCCCAC TAACCAACCG CTCTTCGAGT GGCAATCGCA CCAACAACAA TCCGAGCAAC 
    AACAACAACA ACCAGACCAC AAGCTTCATA ATCGGAGGTA CCACCAATAT TAACAACAAC
    AACAATCAGC TTGGTGGCAC AAACAGCGCT AAGGATAAAG GAAATTTTGA AAATAATTGG
    AGCTGCAATA AAACAACCGG TCGTCGATCT GGCAGTGGAA ATAGTTGCAG TTTTTTAGAT
    CTCCAGTCCA TCCGACGACT CCTGGAGCAC GATGCCAGTG GATCCGTTTG CCCCTCCTGC
    CGCATATCCT TCGACAAGGG CAAGAGACGG AAACTGATCG ACACCTGCGG CCACGAGCGG
    TGCTATTCCT GCATGTTCCG CAACGACCAG TGCCCCATGT GCATGAACAG CTCGCTGAAA
    GATGTTGATG GCGCCAATGC TCAGGGCTAT GACACCGGAA TCGGTGGCTC CACATCGACG
    ATAGTGAGTC CTCTTGGATC CCCGCAGCCG CAGCTCAGAT CGCATGCGCA ACGAAACGCA
    GCGCTGGCGC GCTACATGCA GCACCGCCAG GAGTCGCAGT CGCCAGATTA CCACCATCGC
    TATGCGAGCA ATGGCAAACT GCCCCGGGCG GCGGCACTGT CTACCAATGG CAATGGATTT
    ACCACCGGCA CCACGCAAAC GGTCACAGTG GATGTGCATC ACCAGCTCGG CGGAGGAGGA
    GGAGGTGCTG CCGCCGCGGC CAAGCAGTTG ATGGCCAACG GCATCAGTGG CCATAGTCGC
    AGCAGCAGCA TGAGCCACAA CATCCATGCG GCAGCCTACA GCGAACTGAG TGCCGCACCA
    CCGGCCTTCG CCACACCGCC GACGCGTAGG CGGTTCTTTA ACCACAAGAA TCTGCGCAGT
    GCCCTCACTG GAGGAAGTGG CGGTGGATCG GGAGTCGGCG GTGGTGTAGG AGGAGCTGGC
    GGTGTGGGCG GCGGTCATCG ACGCACTGCC TCCAATGGGG GTTGTCCCAT TGACACAGCA
    TCGATCCTGT CGGGAGGAAG CGACGCTATT GACCACCACT CCTCCTTCCT GAATGAGAAA
    CTGCAGAAGG ATCAGCTCCA CGCCCGTCTC GGTCTGCTGC TCAACGATCC CGGCAGCAAT
    GGAAACAGCA GCAGCAGCGG AAGTGGTTGT GAACCCATCT CGGCCCATTC CACCACCAGC
    ACCACGAGCA GCAGCGGCGT GGGAGCGGCC AGCACCACCA CGAGCGGTTC CTCGCAGAAT
    GTCAGTCCGG AGCAAACGCT GGCCAGCGGA GCCGGAATGC TCAGTGGCAG TCAGCTGTCG
    GTGGCCACCA GTCATGGGGT CAAGGAGGAT GCACTGAGTC TGTGCGGCAA GTTCAAGGCT
    GGCTGCTCCA TGCTGCACGT CTACGAAGCA TTGCCCAGCA AGAGTCGCAA AGGAAATGTA
    CGGAGATCGA CGCGTGGTCA GCAGGGATCC AGTTCGAGCG CATCGGCGGC ATCAGCAGTG
    GGATCCAGGG TGACAGCCTC CAGTTTGGCG GCTGTGCAGT TGGCCCTGAA GCCACTGTTC
    TTTGAGGTGC CGCTGCAGGA ACCGGATCCA CCATATGTGG GTCGCCAGTG GCTGGTGCAG
    CAGTTGTCCA ACATCCTGCT TGGCACCGAG ACACGGGTGG TGCTCATTAA CGGACAGCCG
    GGCACTGGAA AGACGGCGTT TTGTCTGCAG CTGGTGGAGT ACTCCTGCTT TGGCCGCCGG
    CAGATGCAGG ATGATCCCGA TGGCATATAC AGTCAGCTAC AGTTGGGCGC CCACTGTGAG
    CGCATGCGCG GACTGGCCTC CCACATGGTT GGCTATCACT TCTGCCAGGC GGACGCCAAT
    CTCACCTGCC AGGTGCCCGA TTTCGTGCAT TCTCTGGCCG CCCAACTGTG TCAGGCGCCG
    CAGTTAACCG CCTACAGGGA TTACCTGCTC TCGGAGCCGC ATCTTCAGGA CATTCTCAGT
    GTGCGAGAGT GCATCGCGGA TGCGGAGAGA GTAATGAAGC TGGCTATACT GGAACCACTG
    GCTCACCTTC ACCGGGCGGG AAAGATACCC GCCAAAGTGG CCGTCATCGT GGTGGATGCC
    TTGTGCGAAG CGGAGTACCA TCGTCCCGAT CATGGTCACA CCATTGCTAG TTTCTTGGCA
    CAGCTTACGC CACATTTTCC AGCTTGGCTC AAGCTGGTGG CCACTGTGAG GACCCAGATG
    TTGGAGCTGG TGAAGGCACC CAGCTACACA CAACTTACCC TGGACAGTTG GGCCAGCAGC
    CAGGCGTTGC AGCAGGATAT GCTTGATTAC ATAGGCGCTC GGCTTGCAGA TAGTCCGGAA
    ATTCGAATGA ACATCGGCGG CGGAGGAGGC CAGAACTCGC AGTCGGGCAG CCAGCCGCAG
    ACGAAGTTCG TGTCCCATCT TCAGTCCCTG AGCAGGGGCT CCATGCTGTA CGCTAAGCTC
    ATCCTGGATC TCATTGCACG CGGCCAGTTG GTTATCAAGT CATCCAGCTA CAAGGTGCTG
    CCGGTCTCTC TTGCTCAGAT CTTTCTGCTG CACTTCAACC TGCGCTTTCC CACCGCCCGT
    AGCTTTGAGC AGGCTGCTCC GATCCTCAAT ATATGCCTGG CTGCTCTGTA TCCTCTGACG
    CTGGACGAGA TCTACTACAG TATGGAGGCA CTGAGTCACG GACGGGAGGC ACTAAGCTGG
    CCGGACTTCA TGCAACGCTT TAAGCTGCTG GATGGATTTT TAATCAAGAG GTTGGACAAC
    ACGTACATGT TCTTTCATAG CTCCTTGAGG GAGTGGCTCA TGCGAAGGGA TGAGGGGGAG
    TCCAACAAGT TCCTCTGCGA CGCCCGTTTG GGTCATGCTG GAATCGCTTT CCGATTATCC
    CGACTGCAGG CTCCATTGTC ACCTCAGCTC ACATTGGAAC TGGGTCACCA CATGCTGAAA
    GCACATTTAT ACGGAGGAAC TAGTCTGACG CTGCTTTCTC CGCGGGATTT GCAATCTTAT
    TGGTTGGCGG GAGCGGCGGA TAACATTTCA TCCTCGCTGG GCGCCCTGCG AAATGTCTAC
    AGTCCGAACC TAAAGGTTTC CCGACTAGTT CTCTTGGCAG GAGCTTCACC GAATCACCGC
    ACGGATTACA TGGGTGGAGC TCCGATCCTT TGCATAGCTG CGCATGAGGG AATCCTTCCC
    ATGGTTAGCC TGCTCCTAGA ATTCGGTGCC GATGTCGGTC TGACCAACAG CCAGGGCTGT
    ACTCCATTAA TACTGGCCGC CATGCGTGGC CACTGCGACG TAGTGCGTCC ATTGGTAGCC
    GCCGGCAGCA GCCTCGGTCA GTTGGACATC ACGCAGCGCT GTGCCCTAGT TCATGCCGCC
    CGCATGGGTC ACCTCAGTGT GGTCAAGTAC TTGCTCGCCT GCGACTGGTC ACCACGACCG
    CACTCCCAAG ATGTCACCAG ATCGGTTGCC TTGCAACAGG CGCTCATTGG AGCTGCCGCA
    CAGGCGCACT GCAAGATCCT TGAGGATCTT CTGGATCTCA ATGAGACCGA GTTTGACCTG
    GACGTGAATG GCATGGAGCC AAGCAGTGGT GAACTGGCGC TAACCGCCGC CGCACGGCAC
    GGATGCATTG ATGTTGTAGG AATCTTGCTC TCCCGTGGCG CTCAGATCGA TGCAAGAAAT
    CGGCAGGGAT ACTCGGCGCT TTGGTTGGCT GTCAAGGAGG GTCACTGGAG TGTGGTGGAG
    CATCTGCTGC AACGTGGTGC CCTGTTGGAT GAACCATTGG GACAGACACG CAAGACGCCG
    CTAATGATTG CGGCGGAGGA GGGACACCTG GAGCTGGTCG ATCTGCTGCT GGCGCGGGGA
    GCGCAACGAG AAGCTCAGGA TCACGAGGGA TTCACTGCAC TCAGCTGGGC CTGTTTACGT
    GGCCACTTGG CGGCGGCCAA GACGCTCATA GAACATGGCT GTAATCGCCA CCACGAGGAT
    CACAATGGAC GCACAGCCCT GGATCTGGCA GCCTATCAGG GAGCCGCTAG CCTGGTGATC
    TATATCCTGG AGCAGGGCGG CAATCTGGAA CACATTGATG TTCATGGAAT GCGTCCCCTG
    GATCGGGCCA TTGCCTGCCG TAACATCCAG GCCGTACAGG TGTTCCTGCG CAAGGGGGCC
    AAACTGGGAC CGACCACTTG GAGCATGGCC ATGGGCAAGC CGGAGATTCT GGTCATACTA
    CTGAACAAGC TGCTCGAGGA CGGTAATGTG CTGTACCGGA AAAACCGCTT CCAGGAGGCT
    GCTCATCGCT ATCAGTATGC CCTACGCAAG ATCTCCGGAC TGGAGCAGTT GCTCGAAAGG
    AATGCCATCT TCGCCCAGCT GCGGACCAAT CTTCTGCTGA ATCTGTCGCG CTGCAAGCGA
    AAACTGAATG AACTTGACGC CTCCATAGAT TTGGCCACGC AAGCGATTGC CCAGAAGCCG
    CACAGCTACG AAGGATACTA CGCCAGGGCA AAGGCGCGCA TGGAACTTGG TGCATTGAAT
    GAAGCACTTG TGGACGCCAA TGAGGCGATG CAGCAGGCCG CCCAAAGCGG TGTGCTCTGC
    GAAGTGGTCG AGGTGCTGAA GCGCATCCAG ACCGAGCTGC TCACGCGGAT CAGCATCAGC
    AGCGAGGATC AGACCGGCAG GATGTCGAAT ATCGGCGGCG GGGCATCCGT CGCATACGAA
    TCTCATCATG AGATCACCGA TTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261738.1
    CDS523..5229
    Translation

    Target ORF information:

    RefSeq Version NM_001274809.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster rolling pebbles (rols), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001274809.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTTCCCAC TAACCAACCG CTCTTCGAGT GGCAATCGCA CCAACAACAA TCCGAGCAAC 
    AACAACAACA ACCAGACCAC AAGCTTCATA ATCGGAGGTA CCACCAATAT TAACAACAAC
    AACAATCAGC TTGGTGGCAC AAACAGCGCT AAGGATAAAG GAAATTTTGA AAATAATTGG
    AGCTGCAATA AAACAACCGG TCGTCGATCT GGCAGTGGAA ATAGTTGCAG TTTTTTAGAT
    CTCCAGTCCA TCCGACGACT CCTGGAGCAC GATGCCAGTG GATCCGTTTG CCCCTCCTGC
    CGCATATCCT TCGACAAGGG CAAGAGACGG AAACTGATCG ACACCTGCGG CCACGAGCGG
    TGCTATTCCT GCATGTTCCG CAACGACCAG TGCCCCATGT GCATGAACAG CTCGCTGAAA
    GATGTTGATG GCGCCAATGC TCAGGGCTAT GACACCGGAA TCGGTGGCTC CACATCGACG
    ATAGTGAGTC CTCTTGGATC CCCGCAGCCG CAGCTCAGAT CGCATGCGCA ACGAAACGCA
    GCGCTGGCGC GCTACATGCA GCACCGCCAG GAGTCGCAGT CGCCAGATTA CCACCATCGC
    TATGCGAGCA ATGGCAAACT GCCCCGGGCG GCGGCACTGT CTACCAATGG CAATGGATTT
    ACCACCGGCA CCACGCAAAC GGTCACAGTG GATGTGCATC ACCAGCTCGG CGGAGGAGGA
    GGAGGTGCTG CCGCCGCGGC CAAGCAGTTG ATGGCCAACG GCATCAGTGG CCATAGTCGC
    AGCAGCAGCA TGAGCCACAA CATCCATGCG GCAGCCTACA GCGAACTGAG TGCCGCACCA
    CCGGCCTTCG CCACACCGCC GACGCGTAGG CGGTTCTTTA ACCACAAGAA TCTGCGCAGT
    GCCCTCACTG GAGGAAGTGG CGGTGGATCG GGAGTCGGCG GTGGTGTAGG AGGAGCTGGC
    GGTGTGGGCG GCGGTCATCG ACGCACTGCC TCCAATGGGG GTTGTCCCAT TGACACAGCA
    TCGATCCTGT CGGGAGGAAG CGACGCTATT GACCACCACT CCTCCTTCCT GAATGAGAAA
    CTGCAGAAGG ATCAGCTCCA CGCCCGTCTC GGTCTGCTGC TCAACGATCC CGGCAGCAAT
    GGAAACAGCA GCAGCAGCGG AAGTGGTTGT GAACCCATCT CGGCCCATTC CACCACCAGC
    ACCACGAGCA GCAGCGGCGT GGGAGCGGCC AGCACCACCA CGAGCGGTTC CTCGCAGAAT
    GTCAGTCCGG AGCAAACGCT GGCCAGCGGA GCCGGAATGC TCAGTGGCAG TCAGCTGTCG
    GTGGCCACCA GTCATGGGGT CAAGGAGGAT GCACTGAGTC TGTGCGGCAA GTTCAAGGCT
    GGCTGCTCCA TGCTGCACGT CTACGAAGCA TTGCCCAGCA AGAGTCGCAA AGGAAATGTA
    CGGAGATCGA CGCGTGGTCA GCAGGGATCC AGTTCGAGCG CATCGGCGGC ATCAGCAGTG
    GGATCCAGGG TGACAGCCTC CAGTTTGGCG GCTGTGCAGT TGGCCCTGAA GCCACTGTTC
    TTTGAGGTGC CGCTGCAGGA ACCGGATCCA CCATATGTGG GTCGCCAGTG GCTGGTGCAG
    CAGTTGTCCA ACATCCTGCT TGGCACCGAG ACACGGGTGG TGCTCATTAA CGGACAGCCG
    GGCACTGGAA AGACGGCGTT TTGTCTGCAG CTGGTGGAGT ACTCCTGCTT TGGCCGCCGG
    CAGATGCAGG ATGATCCCGA TGGCATATAC AGTCAGCTAC AGTTGGGCGC CCACTGTGAG
    CGCATGCGCG GACTGGCCTC CCACATGGTT GGCTATCACT TCTGCCAGGC GGACGCCAAT
    CTCACCTGCC AGGTGCCCGA TTTCGTGCAT TCTCTGGCCG CCCAACTGTG TCAGGCGCCG
    CAGTTAACCG CCTACAGGGA TTACCTGCTC TCGGAGCCGC ATCTTCAGGA CATTCTCAGT
    GTGCGAGAGT GCATCGCGGA TGCGGAGAGA GTAATGAAGC TGGCTATACT GGAACCACTG
    GCTCACCTTC ACCGGGCGGG AAAGATACCC GCCAAAGTGG CCGTCATCGT GGTGGATGCC
    TTGTGCGAAG CGGAGTACCA TCGTCCCGAT CATGGTCACA CCATTGCTAG TTTCTTGGCA
    CAGCTTACGC CACATTTTCC AGCTTGGCTC AAGCTGGTGG CCACTGTGAG GACCCAGATG
    TTGGAGCTGG TGAAGGCACC CAGCTACACA CAACTTACCC TGGACAGTTG GGCCAGCAGC
    CAGGCGTTGC AGCAGGATAT GCTTGATTAC ATAGGCGCTC GGCTTGCAGA TAGTCCGGAA
    ATTCGAATGA ACATCGGCGG CGGAGGAGGC CAGAACTCGC AGTCGGGCAG CCAGCCGCAG
    ACGAAGTTCG TGTCCCATCT TCAGTCCCTG AGCAGGGGCT CCATGCTGTA CGCTAAGCTC
    ATCCTGGATC TCATTGCACG CGGCCAGTTG GTTATCAAGT CATCCAGCTA CAAGGTGCTG
    CCGGTCTCTC TTGCTCAGAT CTTTCTGCTG CACTTCAACC TGCGCTTTCC CACCGCCCGT
    AGCTTTGAGC AGGCTGCTCC GATCCTCAAT ATATGCCTGG CTGCTCTGTA TCCTCTGACG
    CTGGACGAGA TCTACTACAG TATGGAGGCA CTGAGTCACG GACGGGAGGC ACTAAGCTGG
    CCGGACTTCA TGCAACGCTT TAAGCTGCTG GATGGATTTT TAATCAAGAG GTTGGACAAC
    ACGTACATGT TCTTTCATAG CTCCTTGAGG GAGTGGCTCA TGCGAAGGGA TGAGGGGGAG
    TCCAACAAGT TCCTCTGCGA CGCCCGTTTG GGTCATGCTG GAATCGCTTT CCGATTATCC
    CGACTGCAGG CTCCATTGTC ACCTCAGCTC ACATTGGAAC TGGGTCACCA CATGCTGAAA
    GCACATTTAT ACGGAGGAAC TAGTCTGACG CTGCTTTCTC CGCGGGATTT GCAATCTTAT
    TGGTTGGCGG GAGCGGCGGA TAACATTTCA TCCTCGCTGG GCGCCCTGCG AAATGTCTAC
    AGTCCGAACC TAAAGGTTTC CCGACTAGTT CTCTTGGCAG GAGCTTCACC GAATCACCGC
    ACGGATTACA TGGGTGGAGC TCCGATCCTT TGCATAGCTG CGCATGAGGG AATCCTTCCC
    ATGGTTAGCC TGCTCCTAGA ATTCGGTGCC GATGTCGGTC TGACCAACAG CCAGGGCTGT
    ACTCCATTAA TACTGGCCGC CATGCGTGGC CACTGCGACG TAGTGCGTCC ATTGGTAGCC
    GCCGGCAGCA GCCTCGGTCA GTTGGACATC ACGCAGCGCT GTGCCCTAGT TCATGCCGCC
    CGCATGGGTC ACCTCAGTGT GGTCAAGTAC TTGCTCGCCT GCGACTGGTC ACCACGACCG
    CACTCCCAAG ATGTCACCAG ATCGGTTGCC TTGCAACAGG CGCTCATTGG AGCTGCCGCA
    CAGGCGCACT GCAAGATCCT TGAGGATCTT CTGGATCTCA ATGAGACCGA GTTTGACCTG
    GACGTGAATG GCATGGAGCC AAGCAGTGGT GAACTGGCGC TAACCGCCGC CGCACGGCAC
    GGATGCATTG ATGTTGTAGG AATCTTGCTC TCCCGTGGCG CTCAGATCGA TGCAAGAAAT
    CGGCAGGGAT ACTCGGCGCT TTGGTTGGCT GTCAAGGAGG GTCACTGGAG TGTGGTGGAG
    CATCTGCTGC AACGTGGTGC CCTGTTGGAT GAACCATTGG GACAGACACG CAAGACGCCG
    CTAATGATTG CGGCGGAGGA GGGACACCTG GAGCTGGTCG ATCTGCTGCT GGCGCGGGGA
    GCGCAACGAG AAGCTCAGGA TCACGAGGGA TTCACTGCAC TCAGCTGGGC CTGTTTACGT
    GGCCACTTGG CGGCGGCCAA GACGCTCATA GAACATGGCT GTAATCGCCA CCACGAGGAT
    CACAATGGAC GCACAGCCCT GGATCTGGCA GCCTATCAGG GAGCCGCTAG CCTGGTGATC
    TATATCCTGG AGCAGGGCGG CAATCTGGAA CACATTGATG TTCATGGAAT GCGTCCCCTG
    GATCGGGCCA TTGCCTGCCG TAACATCCAG GCCGTACAGG TGTTCCTGCG CAAGGGGGCC
    AAACTGGGAC CGACCACTTG GAGCATGGCC ATGGGCAAGC CGGAGATTCT GGTCATACTA
    CTGAACAAGC TGCTCGAGGA CGGTAATGTG CTGTACCGGA AAAACCGCTT CCAGGAGGCT
    GCTCATCGCT ATCAGTATGC CCTACGCAAG ATCTCCGGAC TGGAGCAGTT GCTCGAAAGG
    AATGCCATCT TCGCCCAGCT GCGGACCAAT CTTCTGCTGA ATCTGTCGCG CTGCAAGCGA
    AAACTGAATG AACTTGACGC CTCCATAGAT TTGGCCACGC AAGCGATTGC CCAGAAGCCG
    CACAGCTACG AAGGATACTA CGCCAGGGCA AAGGCGCGCA TGGAACTTGG TGCATTGAAT
    GAAGCACTTG TGGACGCCAA TGAGGCGATG CAGCAGGCCG CCCAAAGCGG TGTGCTCTGC
    GAAGTGGTCG AGGTGCTGAA GCGCATCCAG ACCGAGCTGC TCACGCGGAT CAGCATCAGC
    AGCGAGGATC AGACCGGCAG GATGTCGAAT ATCGGCGGCG GGGCATCCGT CGCATACGAA
    TCTCATCATG AGATCACCGA TTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa18308
    Clone ID Related Accession (Same CDS sequence) NM_001274807.1
    Accession Version NM_001274807.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5010bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product rolling pebbles, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTTCCCAC TAACCAACCG CTCTTCGAGT GGCAATCGCA CCAACAACAA TCCGAGCAAC 
    AACAACAACA ACCAGACCAC AAGCTTCATA ATCGGAGGTA CCACCAATAT TAACAACAAC
    AACAATCAGC TTGGTGGCAC AAACAGCGCT AAGGATAAAG GAAATTTTGA AAATAATTGG
    AGCTGCAATA AAACAACCGG TCGTCGATCT GGCAGTGGAA ATAGTTGCAG TTTTTTAGAT
    CTCCAGTCCA TCCGACGACT CCTGGAGCAC GATGCCAGTG GATCCGTTTG CCCCTCCTGC
    CGCATATCCT TCGACAAGGG CAAGAGACGG AAACTGATCG ACACCTGCGG CCACGAGCGG
    TGCTATTCCT GCATGTTCCG CAACGACCAG TGCCCCATGT GCATGAACAG CTCGCTGAAA
    GATGTTGATG GCGCCAATGC TCAGGGCTAT GACACCGGAA TCGGTGGCTC CACATCGACG
    ATAGTGAGTC CTCTTGGATC CCCGCAGCCG CAGCTCAGAT CGCATGCGCA ACGAAACGCA
    GCGCTGGCGC GCTACATGCA GCACCGCCAG GAGTCGCAGT CGCCAGATTA CCACCATCGC
    TATGCGAGCA ATGGCAAACT GCCCCGGGCG GCGGCACTGT CTACCAATGG CAATGGATTT
    ACCACCGGCA CCACGCAAAC GGTCACAGTG GATGTGCATC ACCAGCTCGG CGGAGGAGGA
    GGAGGTGCTG CCGCCGCGGC CAAGCAGTTG ATGGCCAACG GCATCAGTGG CCATAGTCGC
    AGCAGCAGCA TGAGCCACAA CATCCATGCG GCAGCCTACA GCGAACTGAG TGCCGCACCA
    CCGGCCTTCG CCACACCGCC GACGCGTAGG CGGTTCTTTA ACCACAAGAA TCTGCGCAGT
    GCCCTCACTG GAGGAAGTGG CGGTGGATCG GGAGTCGGCG GTGGTGTAGG AGGAGCTGGC
    GGTGTGGGCG GCGGTCATCG ACGCACTGCC TCCAATGGGG GTTGTCCCAT TGACACAGCA
    TCGATCCTGT CGGGAGATCA TACTCATCAG CATCATCACC GCGACAATCA GCCGGAGGGC
    AAGGAGTCCA ATGCCCTTAA CACATCCACC TGCTCGGACT CGGCGGTCAC TAGGAGGCGG
    AGGAAGAACG TGAGCAATCA CAACCTGAAG ACCTCTGCCC GCCATGGAGC AAGTAGCGAG
    AATCGTCTGA ACAGGCTCAG TTTGGCCGGA ACGTCCGTCT ATGCGGGTCA TCTGTCCTCT
    TTGGTCTTTG GCAAGATCAA GTCCCTGTGG AGCGTGAACT CCTCCAATTC CAGCGAGGCG
    GGACTGAATC AATTAGCAGG AAGCGACGCT ATTGACCACC ACTCCTCCTT CCTGAATGAG
    AAACTGCAGA AGGATCAGCT CCACGCCCGT CTCGGTCTGC TGCTCAACGA TCCCGGCAGC
    AATGGAAACA GCAGCAGCAG CGGAAGTGGT TGTGAACCCA TCTCGGCCCA TTCCACCACC
    AGCACCACGA GCAGCAGCGG CGTGGGAGCG GCCAGCACCA CCACGAGCGG TTCCTCGCAG
    AATGTCAGTC CGGAGCAAAC GCTGGCCAGC GGAGCCGGAA TGCTCAGTGG CAGTCAGCTG
    TCGGTGGCCA CCAGTCATGG GGTCAAGGAG GATGCACTGA GTCTGTGCGG CAAGTTCAAG
    GCTGGCTGCT CCATGCTGCA CGTCTACGAA GCATTGCCCA GCAAGAGTCG CAAAGGAAAT
    GTACGGAGAT CGACGCGTGG TCAGCAGGGA TCCAGTTCGA GCGCATCGGC GGCATCAGCA
    GTGGGATCCA GGGTGACAGC CTCCAGTTTG GCGGCTGTGC AGTTGGCCCT GAAGCCACTG
    TTCTTTGAGG TGCCGCTGCA GGAACCGGAT CCACCATATG TGGGTCGCCA GTGGCTGGTG
    CAGCAGTTGT CCAACATCCT GCTTGGCACC GAGACACGGG TGGTGCTCAT TAACGGACAG
    CCGGGCACTG GAAAGACGGC GTTTTGTCTG CAGCTGGTGG AGTACTCCTG CTTTGGCCGC
    CGGCAGATGC AGGATGATCC CGATGGCATA TACAGTCAGC TACAGTTGGG CGCCCACTGT
    GAGCGCATGC GCGGACTGGC CTCCCACATG GTTGGCTATC ACTTCTGCCA GGCGGACGCC
    AATCTCACCT GCCAGGTGCC CGATTTCGTG CATTCTCTGG CCGCCCAACT GTGTCAGGCG
    CCGCAGTTAA CCGCCTACAG GGATTACCTG CTCTCGGAGC CGCATCTTCA GGACATTCTC
    AGTGTGCGAG AGTGCATCGC GGATGCGGAG AGAGTAATGA AGCTGGCTAT ACTGGAACCA
    CTGGCTCACC TTCACCGGGC GGGAAAGATA CCCGCCAAAG TGGCCGTCAT CGTGGTGGAT
    GCCTTGTGCG AAGCGGAGTA CCATCGTCCC GATCATGGTC ACACCATTGC TAGTTTCTTG
    GCACAGCTTA CGCCACATTT TCCAGCTTGG CTCAAGCTGG TGGCCACTGT GAGGACCCAG
    ATGTTGGAGC TGGTGAAGGC ACCCAGCTAC ACACAACTTA CCCTGGACAG TTGGGCCAGC
    AGCCAGGCGT TGCAGCAGGA TATGCTTGAT TACATAGGCG CTCGGCTTGC AGATAGTCCG
    GAAATTCGAA TGAACATCGG CGGCGGAGGA GGCCAGAACT CGCAGTCGGG CAGCCAGCCG
    CAGACGAAGT TCGTGTCCCA TCTTCAGTCC CTGAGCAGGG GCTCCATGCT GTACGCTAAG
    CTCATCCTGG ATCTCATTGC ACGCGGCCAG TTGGTTATCA AGTCATCCAG CTACAAGGTG
    CTGCCGGTCT CTCTTGCTCA GATCTTTCTG CTGCACTTCA ACCTGCGCTT TCCCACCGCC
    CGTAGCTTTG AGCAGGCTGC TCCGATCCTC AATATATGCC TGGCTGCTCT GTATCCTCTG
    ACGCTGGACG AGATCTACTA CAGTATGGAG GCACTGAGTC ACGGACGGGA GGCACTAAGC
    TGGCCGGACT TCATGCAACG CTTTAAGCTG CTGGATGGAT TTTTAATCAA GAGGTTGGAC
    AACACGTACA TGTTCTTTCA TAGCTCCTTG AGGGAGTGGC TCATGCGAAG GGATGAGGGG
    GAGTCCAACA AGTTCCTCTG CGACGCCCGT TTGGGTCATG CTGGAATCGC TTTCCGATTA
    TCCCGACTGC AGGCTCCATT GTCACCTCAG CTCACATTGG AACTGGGTCA CCACATGCTG
    AAAGCACATT TATACGGAGG AACTAGTCTG ACGCTGCTTT CTCCGCGGGA TTTGCAATCT
    TATTGGTTGG CGGGAGCGGC GGATAACATT TCATCCTCGC TGGGCGCCCT GCGAAATGTC
    TACAGTCCGA ACCTAAAGGT TTCCCGACTA GTTCTCTTGG CAGGAGCTTC ACCGAATCAC
    CGCACGGATT ACATGGGTGG AGCTCCGATC CTTTGCATAG CTGCGCATGA GGGAATCCTT
    CCCATGGTTA GCCTGCTCCT AGAATTCGGT GCCGATGTCG GTCTGACCAA CAGCCAGGGC
    TGTACTCCAT TAATACTGGC CGCCATGCGT GGCCACTGCG ACGTAGTGCG TCCATTGGTA
    GCCGCCGGCA GCAGCCTCGG TCAGTTGGAC ATCACGCAGC GCTGTGCCCT AGTTCATGCC
    GCCCGCATGG GTCACCTCAG TGTGGTCAAG TACTTGCTCG CCTGCGACTG GTCACCACGA
    CCGCACTCCC AAGATGTCAC CAGATCGGTT GCCTTGCAAC AGGCGCTCAT TGGAGCTGCC
    GCACAGGCGC ACTGCAAGAT CCTTGAGGAT CTTCTGGATC TCAATGAGAC CGAGTTTGAC
    CTGGACGTGA ATGGCATGGA GCCAAGCAGT GGTGAACTGG CGCTAACCGC CGCCGCACGG
    CACGGATGCA TTGATGTTGT AGGAATCTTG CTCTCCCGTG GCGCTCAGAT CGATGCAAGA
    AATCGGCAGG GATACTCGGC GCTTTGGTTG GCTGTCAAGG AGGGTCACTG GAGTGTGGTG
    GAGCATCTGC TGCAACGTGG TGCCCTGTTG GATGAACCAT TGGGACAGAC ACGCAAGACG
    CCGCTAATGA TTGCGGCGGA GGAGGGACAC CTGGAGCTGG TCGATCTGCT GCTGGCGCGG
    GGAGCGCAAC GAGAAGCTCA GGATCACGAG GGATTCACTG CACTCAGCTG GGCCTGTTTA
    CGTGGCCACT TGGCGGCGGC CAAGACGCTC ATAGAACATG GCTGTAATCG CCACCACGAG
    GATCACAATG GACGCACAGC CCTGGATCTG GCAGCCTATC AGGGAGCCGC TAGCCTGGTG
    ATCTATATCC TGGAGCAGGG CGGCAATCTG GAACACATTG ATGTTCATGG AATGCGTCCC
    CTGGATCGGG CCATTGCCTG CCGTAACATC CAGGCCGTAC AGGTGTTCCT GCGCAAGGGG
    GCCAAACTGG GACCGACCAC TTGGAGCATG GCCATGGGCA AGCCGGAGAT TCTGGTCATA
    CTACTGAACA AGCTGCTCGA GGACGGTAAT GTGCTGTACC GGAAAAACCG CTTCCAGGAG
    GCTGCTCATC GCTATCAGTA TGCCCTACGC AAGATCTCCG GACTGGAGCA GTTGCTCGAA
    AGGAATGCCA TCTTCGCCCA GCTGCGGACC AATCTTCTGC TGAATCTGTC GCGCTGCAAG
    CGAAAACTGA ATGAACTTGA CGCCTCCATA GATTTGGCCA CGCAAGCGAT TGCCCAGAAG
    CCGCACAGCT ACGAAGGATA CTACGCCAGG GCAAAGGCGC GCATGGAACT TGGTGCATTG
    AATGAAGCAC TTGTGGACGC CAATGAGGCG ATGCAGCAGG CCGCCCAAAG CGGTGTGCTC
    TGCGAAGTGG TCGAGGTGCT GAAGCGCATC CAGACCGAGC TGCTCACGCG GATCAGCATC
    AGCAGCGAGG ATCAGACCGG CAGGATGTCG AATATCGGCG GCGGGGCATC CGTCGCATAC
    GAATCTCATC ATGAGATCAC CGATTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001261736.1
    CDS564..5573
    Translation

    Target ORF information:

    RefSeq Version NM_001274807.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster rolling pebbles (rols), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001274807.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTTCCCAC TAACCAACCG CTCTTCGAGT GGCAATCGCA CCAACAACAA TCCGAGCAAC 
    AACAACAACA ACCAGACCAC AAGCTTCATA ATCGGAGGTA CCACCAATAT TAACAACAAC
    AACAATCAGC TTGGTGGCAC AAACAGCGCT AAGGATAAAG GAAATTTTGA AAATAATTGG
    AGCTGCAATA AAACAACCGG TCGTCGATCT GGCAGTGGAA ATAGTTGCAG TTTTTTAGAT
    CTCCAGTCCA TCCGACGACT CCTGGAGCAC GATGCCAGTG GATCCGTTTG CCCCTCCTGC
    CGCATATCCT TCGACAAGGG CAAGAGACGG AAACTGATCG ACACCTGCGG CCACGAGCGG
    TGCTATTCCT GCATGTTCCG CAACGACCAG TGCCCCATGT GCATGAACAG CTCGCTGAAA
    GATGTTGATG GCGCCAATGC TCAGGGCTAT GACACCGGAA TCGGTGGCTC CACATCGACG
    ATAGTGAGTC CTCTTGGATC CCCGCAGCCG CAGCTCAGAT CGCATGCGCA ACGAAACGCA
    GCGCTGGCGC GCTACATGCA GCACCGCCAG GAGTCGCAGT CGCCAGATTA CCACCATCGC
    TATGCGAGCA ATGGCAAACT GCCCCGGGCG GCGGCACTGT CTACCAATGG CAATGGATTT
    ACCACCGGCA CCACGCAAAC GGTCACAGTG GATGTGCATC ACCAGCTCGG CGGAGGAGGA
    GGAGGTGCTG CCGCCGCGGC CAAGCAGTTG ATGGCCAACG GCATCAGTGG CCATAGTCGC
    AGCAGCAGCA TGAGCCACAA CATCCATGCG GCAGCCTACA GCGAACTGAG TGCCGCACCA
    CCGGCCTTCG CCACACCGCC GACGCGTAGG CGGTTCTTTA ACCACAAGAA TCTGCGCAGT
    GCCCTCACTG GAGGAAGTGG CGGTGGATCG GGAGTCGGCG GTGGTGTAGG AGGAGCTGGC
    GGTGTGGGCG GCGGTCATCG ACGCACTGCC TCCAATGGGG GTTGTCCCAT TGACACAGCA
    TCGATCCTGT CGGGAGATCA TACTCATCAG CATCATCACC GCGACAATCA GCCGGAGGGC
    AAGGAGTCCA ATGCCCTTAA CACATCCACC TGCTCGGACT CGGCGGTCAC TAGGAGGCGG
    AGGAAGAACG TGAGCAATCA CAACCTGAAG ACCTCTGCCC GCCATGGAGC AAGTAGCGAG
    AATCGTCTGA ACAGGCTCAG TTTGGCCGGA ACGTCCGTCT ATGCGGGTCA TCTGTCCTCT
    TTGGTCTTTG GCAAGATCAA GTCCCTGTGG AGCGTGAACT CCTCCAATTC CAGCGAGGCG
    GGACTGAATC AATTAGCAGG AAGCGACGCT ATTGACCACC ACTCCTCCTT CCTGAATGAG
    AAACTGCAGA AGGATCAGCT CCACGCCCGT CTCGGTCTGC TGCTCAACGA TCCCGGCAGC
    AATGGAAACA GCAGCAGCAG CGGAAGTGGT TGTGAACCCA TCTCGGCCCA TTCCACCACC
    AGCACCACGA GCAGCAGCGG CGTGGGAGCG GCCAGCACCA CCACGAGCGG TTCCTCGCAG
    AATGTCAGTC CGGAGCAAAC GCTGGCCAGC GGAGCCGGAA TGCTCAGTGG CAGTCAGCTG
    TCGGTGGCCA CCAGTCATGG GGTCAAGGAG GATGCACTGA GTCTGTGCGG CAAGTTCAAG
    GCTGGCTGCT CCATGCTGCA CGTCTACGAA GCATTGCCCA GCAAGAGTCG CAAAGGAAAT
    GTACGGAGAT CGACGCGTGG TCAGCAGGGA TCCAGTTCGA GCGCATCGGC GGCATCAGCA
    GTGGGATCCA GGGTGACAGC CTCCAGTTTG GCGGCTGTGC AGTTGGCCCT GAAGCCACTG
    TTCTTTGAGG TGCCGCTGCA GGAACCGGAT CCACCATATG TGGGTCGCCA GTGGCTGGTG
    CAGCAGTTGT CCAACATCCT GCTTGGCACC GAGACACGGG TGGTGCTCAT TAACGGACAG
    CCGGGCACTG GAAAGACGGC GTTTTGTCTG CAGCTGGTGG AGTACTCCTG CTTTGGCCGC
    CGGCAGATGC AGGATGATCC CGATGGCATA TACAGTCAGC TACAGTTGGG CGCCCACTGT
    GAGCGCATGC GCGGACTGGC CTCCCACATG GTTGGCTATC ACTTCTGCCA GGCGGACGCC
    AATCTCACCT GCCAGGTGCC CGATTTCGTG CATTCTCTGG CCGCCCAACT GTGTCAGGCG
    CCGCAGTTAA CCGCCTACAG GGATTACCTG CTCTCGGAGC CGCATCTTCA GGACATTCTC
    AGTGTGCGAG AGTGCATCGC GGATGCGGAG AGAGTAATGA AGCTGGCTAT ACTGGAACCA
    CTGGCTCACC TTCACCGGGC GGGAAAGATA CCCGCCAAAG TGGCCGTCAT CGTGGTGGAT
    GCCTTGTGCG AAGCGGAGTA CCATCGTCCC GATCATGGTC ACACCATTGC TAGTTTCTTG
    GCACAGCTTA CGCCACATTT TCCAGCTTGG CTCAAGCTGG TGGCCACTGT GAGGACCCAG
    ATGTTGGAGC TGGTGAAGGC ACCCAGCTAC ACACAACTTA CCCTGGACAG TTGGGCCAGC
    AGCCAGGCGT TGCAGCAGGA TATGCTTGAT TACATAGGCG CTCGGCTTGC AGATAGTCCG
    GAAATTCGAA TGAACATCGG CGGCGGAGGA GGCCAGAACT CGCAGTCGGG CAGCCAGCCG
    CAGACGAAGT TCGTGTCCCA TCTTCAGTCC CTGAGCAGGG GCTCCATGCT GTACGCTAAG
    CTCATCCTGG ATCTCATTGC ACGCGGCCAG TTGGTTATCA AGTCATCCAG CTACAAGGTG
    CTGCCGGTCT CTCTTGCTCA GATCTTTCTG CTGCACTTCA ACCTGCGCTT TCCCACCGCC
    CGTAGCTTTG AGCAGGCTGC TCCGATCCTC AATATATGCC TGGCTGCTCT GTATCCTCTG
    ACGCTGGACG AGATCTACTA CAGTATGGAG GCACTGAGTC ACGGACGGGA GGCACTAAGC
    TGGCCGGACT TCATGCAACG CTTTAAGCTG CTGGATGGAT TTTTAATCAA GAGGTTGGAC
    AACACGTACA TGTTCTTTCA TAGCTCCTTG AGGGAGTGGC TCATGCGAAG GGATGAGGGG
    GAGTCCAACA AGTTCCTCTG CGACGCCCGT TTGGGTCATG CTGGAATCGC TTTCCGATTA
    TCCCGACTGC AGGCTCCATT GTCACCTCAG CTCACATTGG AACTGGGTCA CCACATGCTG
    AAAGCACATT TATACGGAGG AACTAGTCTG ACGCTGCTTT CTCCGCGGGA TTTGCAATCT
    TATTGGTTGG CGGGAGCGGC GGATAACATT TCATCCTCGC TGGGCGCCCT GCGAAATGTC
    TACAGTCCGA ACCTAAAGGT TTCCCGACTA GTTCTCTTGG CAGGAGCTTC ACCGAATCAC
    CGCACGGATT ACATGGGTGG AGCTCCGATC CTTTGCATAG CTGCGCATGA GGGAATCCTT
    CCCATGGTTA GCCTGCTCCT AGAATTCGGT GCCGATGTCG GTCTGACCAA CAGCCAGGGC
    TGTACTCCAT TAATACTGGC CGCCATGCGT GGCCACTGCG ACGTAGTGCG TCCATTGGTA
    GCCGCCGGCA GCAGCCTCGG TCAGTTGGAC ATCACGCAGC GCTGTGCCCT AGTTCATGCC
    GCCCGCATGG GTCACCTCAG TGTGGTCAAG TACTTGCTCG CCTGCGACTG GTCACCACGA
    CCGCACTCCC AAGATGTCAC CAGATCGGTT GCCTTGCAAC AGGCGCTCAT TGGAGCTGCC
    GCACAGGCGC ACTGCAAGAT CCTTGAGGAT CTTCTGGATC TCAATGAGAC CGAGTTTGAC
    CTGGACGTGA ATGGCATGGA GCCAAGCAGT GGTGAACTGG CGCTAACCGC CGCCGCACGG
    CACGGATGCA TTGATGTTGT AGGAATCTTG CTCTCCCGTG GCGCTCAGAT CGATGCAAGA
    AATCGGCAGG GATACTCGGC GCTTTGGTTG GCTGTCAAGG AGGGTCACTG GAGTGTGGTG
    GAGCATCTGC TGCAACGTGG TGCCCTGTTG GATGAACCAT TGGGACAGAC ACGCAAGACG
    CCGCTAATGA TTGCGGCGGA GGAGGGACAC CTGGAGCTGG TCGATCTGCT GCTGGCGCGG
    GGAGCGCAAC GAGAAGCTCA GGATCACGAG GGATTCACTG CACTCAGCTG GGCCTGTTTA
    CGTGGCCACT TGGCGGCGGC CAAGACGCTC ATAGAACATG GCTGTAATCG CCACCACGAG
    GATCACAATG GACGCACAGC CCTGGATCTG GCAGCCTATC AGGGAGCCGC TAGCCTGGTG
    ATCTATATCC TGGAGCAGGG CGGCAATCTG GAACACATTG ATGTTCATGG AATGCGTCCC
    CTGGATCGGG CCATTGCCTG CCGTAACATC CAGGCCGTAC AGGTGTTCCT GCGCAAGGGG
    GCCAAACTGG GACCGACCAC TTGGAGCATG GCCATGGGCA AGCCGGAGAT TCTGGTCATA
    CTACTGAACA AGCTGCTCGA GGACGGTAAT GTGCTGTACC GGAAAAACCG CTTCCAGGAG
    GCTGCTCATC GCTATCAGTA TGCCCTACGC AAGATCTCCG GACTGGAGCA GTTGCTCGAA
    AGGAATGCCA TCTTCGCCCA GCTGCGGACC AATCTTCTGC TGAATCTGTC GCGCTGCAAG
    CGAAAACTGA ATGAACTTGA CGCCTCCATA GATTTGGCCA CGCAAGCGAT TGCCCAGAAG
    CCGCACAGCT ACGAAGGATA CTACGCCAGG GCAAAGGCGC GCATGGAACT TGGTGCATTG
    AATGAAGCAC TTGTGGACGC CAATGAGGCG ATGCAGCAGG CCGCCCAAAG CGGTGTGCTC
    TGCGAAGTGG TCGAGGTGCT GAAGCGCATC CAGACCGAGC TGCTCACGCG GATCAGCATC
    AGCAGCGAGG ATCAGACCGG CAGGATGTCG AATATCGGCG GCGGGGCATC CGTCGCATAC
    GAATCTCATC ATGAGATCAC CGATTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa18309
    Clone ID Related Accession (Same CDS sequence) NM_140285.3 , NM_168488.2
    Accession Version NM_140285.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product rolling pebbles, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140285.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTTCCCAC TAACCAACCG CTCTTCGAGT GGCAATCGCA CCAACAACAA TCCGAGCAAC 
    AACAACAACA ACCAGACCAC AAGCTTCATA ATCGGAGGTA CCACCAATAT TAACAACAAC
    AACAATCAGC TTGGTGGCAC AAACAGCGCT AAGGATAAAG GAAATTTTGA AAATAATTGG
    AGCTGCAATA AAACAACCGG TCGTCGATCT GGCAGTGGAA ATAGTTGCAG TTTTTTAGAT
    CTCCAGTCCA TCCGACGACT CCTGGAGCAC GATGCCAGTG GATCCGTTTG CCCCTCCTGC
    CGCATATCCT TCGACAAGGG CAAGAGACGG AAACTGATCG ACACCTGCGG CCACGAGCGG
    TGCTATTCCT GCATGTTCCG CAACGACCAG TGCCCCATGT GCATGAACAG CTCGCTGAAA
    GATGTTGATG GCGCCAATGC TCAGGGCTAT GACACCGGAA TCGGTGGCTC CACATCGACG
    ATAGTGAGTC CTCTTGGATC CCCGCAGCCG CAGCTCAGAT CGCATGCGCA ACGAAACGCA
    GCGCTGGCGC GCTACATGCA GCAGCACCGC CAGGAGTCGC AGTCGCCAGA TTACCACCAT
    CGCTATGCGA GCAATGGCAA ACTGCCCCGG GCGGCGGCAC TGTCTACCAA TGGCAATGGA
    TTTACCACCG GCACCACGCA AACGGTCACA GTGGATGTGC ATCACCAGCT CGGCGGAGGA
    GGAGGAGGTG CTGCCGCCGC GGCCAAGCAG TTGATGGCCA ACGGCATCAG TGGCCATAGT
    CGCAGCAGCA GCATGAGCCA CAACATCCAT GCGGCAGCCT ACAGCGAACT GAGTGCCGCA
    CCACCGGCCT TCGCCACACC GCCGACGCGT AGGCGGTTCT TTAACCACAA GAATCTGCGC
    AGTGCCCTCA CTGGAGGAAG TGGCGGTGGA TCGGGAGTCG GCGGTGGTGT AGGAGGAGCT
    GGCGGTGTGG GCGGCGGTCA TCGACGCACT GCCTCCAATG GGGGTTGTCC CATTGACACA
    GCATCGATCC TGTCGGGAGG AAGCGACGCT ATTGACCACC ACTCCTCCTT CCTGAATGAG
    AAACTGCAGA AGGATCAGCT CCACGCCCGT CTCGGTCTGC TGCTCAACGA TCCCGGCAGC
    AATGGAAACA GCAGCAGCAG CGGAAGTGGT TGTGAACCCA TCTCGGCCCA TTCCACCACC
    AGCACCACGA GCAGCAGCGG CGTGGGAGCG GCCAGCACCA CCACGAGCGG TTCCTCGCAG
    AATGTCAGTC CGGAGCAAAC GCTGGCCAGC GGAGCCGGAA TGCTCAGTGG CAGTCAGCTG
    TCGGTGGCCA CCAGTCATGG GGTCAAGGAG GATGCACTGA GTCTGTGCGG CAAGTTCAAG
    GCTGGCTGCT CCATGCTGCA CGTCTACGAA GCATTGCCCA GCAAGAGTCG CAAAGGAAAT
    GTACGGAGAT CGACGCGTGG TCAGCAGGGA TCCAGTTCGA GCGCATCGGC GGCATCAGCA
    GTGGGATCCA GGGTGACAGC CTCCAGTTTG GCGGCTGTGC AGTTGGCCCT GAAGCCACTG
    TTCTTTGAGG TGCCGCTGCA GGAACCGGAT CCACCATATG TGGGTCGCCA GTGGCTGGTG
    CAGCAGTTGT CCAACATCCT GCTTGGCACC GAGACACGGG TGGTGCTCAT TAACGGACAG
    CCGGGCACTG GAAAGACGGC GTTTTGTCTG CAGCTGGTGG AGTACTCCTG CTTTGGCCGC
    CGGCAGATGC AGGATGATCC CGATGGCATA TACAGTCAGC TACAGTTGGG CGCCCACTGT
    GAGCGCATGC GCGGACTGGC CTCCCACATG GTTGGCTATC ACTTCTGCCA GGCGGACGCC
    AATCTCACCT GCCAGGTGCC CGATTTCGTG CATTCTCTGG CCGCCCAACT GTGTCAGGCG
    CCGCAGTTAA CCGCCTACAG GGATTACCTG CTCTCGGAGC CGCATCTTCA GGACATTCTC
    AGTGTGCGAG AGTGCATCGC GGATGCGGAG AGAGTAATGA AGCTGGCTAT ACTGGAACCA
    CTGGCTCACC TTCACCGGGC GGGAAAGATA CCCGCCAAAG TGGCCGTCAT CGTGGTGGAT
    GCCTTGTGCG AAGCGGAGTA CCATCGTCCC GATCATGGTC ACACCATTGC TAGTTTCTTG
    GCACAGCTTA CGCCACATTT TCCAGCTTGG CTCAAGCTGG TGGCCACTGT GAGGACCCAG
    ATGTTGGAGC TGGTGAAGGC ACCCAGCTAC ACACAACTTA CCCTGGACAG TTGGGCCAGC
    AGCCAGGCGT TGCAGCAGGA TATGCTTGAT TACATAGGCG CTCGGCTTGC AGATAGTCCG
    GAAATTCGAA TGAACATCGG CGGCGGAGGA GGCCAGAACT CGCAGTCGGG CAGCCAGCCG
    CAGACGAAGT TCGTGTCCCA TCTTCAGTCC CTGAGCAGGG GCTCCATGCT GTACGCTAAG
    CTCATCCTGG ATCTCATTGC ACGCGGCCAG TTGGTTATCA AGTCATCCAG CTACAAGGTG
    CTGCCGGTCT CTCTTGCTCA GATCTTTCTG CTGCACTTCA ACCTGCGCTT TCCCACCGCC
    CGTAGCTTTG AGCAGGCTGC TCCGATCCTC AATATATGCC TGGCTGCTCT GTATCCTCTG
    ACGCTGGACG AGATCTACTA CAGTATGGAG GCACTGAGTC ACGGACGGGA GGCACTAAGC
    TGGCCGGACT TCATGCAACG CTTTAAGCTG CTGGATGGAT TTTTAATCAA GAGGTTGGAC
    AACACGTACA TGTTCTTTCA TAGCTCCTTG AGGGAGTGGC TCATGCGAAG GGATGAGGGG
    GAGTCCAACA AGTTCCTCTG CGACGCCCGT TTGGGTCATG CTGGAATCGC TTTCCGATTA
    TCCCGACTGC AGGCTCCATT GTCACCTCAG CTCACATTGG AACTGGGTCA CCACATGCTG
    AAAGCACATT TATACGGAGG AACTAGTCTG ACGCTGCTTT CTCCGCGGGA TTTGCAATCT
    TATTGGTTGG CGGGAGCGGC GGATAACATT TCATCCTCGC TGGGCGCCCT GCGAAATGTC
    TACAGTCCGA ACCTAAAGGT TTCCCGACTA GTTCTCTTGG CAGGAGCTTC ACCGAATCAC
    CGCACGGATT ACATGGGTGG AGCTCCGATC CTTTGCATAG CTGCGCATGA GGGAATCCTT
    CCCATGGTTA GCCTGCTCCT AGAATTCGGT GCCGATGTCG GTCTGACCAA CAGCCAGGGC
    TGTACTCCAT TAATACTGGC CGCCATGCGT GGCCACTGCG ACGTAGTGCG TCCATTGGTA
    GCCGCCGGCA GCAGCCTCGG TCAGTTGGAC ATCACGCAGC GCTGTGCCCT AGTTCATGCC
    GCCCGCATGG GTCACCTCAG TGTGGTCAAG TACTTGCTCG CCTGCGACTG GTCACCACGA
    CCGCACTCCC AAGATGTCAC CAGATCGGTT GCCTTGCAAC AGGCGCTCAT TGGAGCTGCC
    GCACAGGCGC ACTGCAAGAT CCTTGAGGAT CTTCTGGATC TCAATGAGAC CGAGTTTGAC
    CTGGACGTGA ATGGCATGGA GCCAAGCAGT GGTGAACTGG CGCTAACCGC CGCCGCACGG
    CACGGATGCA TTGATGTTGT AGGAATCTTG CTCTCCCGTG GCGCTCAGAT CGATGCAAGA
    AATCGGCAGG GATACTCGGC GCTTTGGTTG GCTGTCAAGG AGGGTCACTG GAGTGTGGTG
    GAGCATCTGC TGCAACGTGG TGCCCTGTTG GATGAACCAT TGGGACAGAC ACGCAAGACG
    CCGCTAATGA TTGCGGCGGA GGAGGGACAC CTGGAGCTGG TCGATCTGCT GCTGGCGCGG
    GGAGCGCAAC GAGAAGCTCA GGATCACGAG GGATTCACTG CACTCAGCTG GGCCTGTTTA
    CGTGGCCACT TGGCGGCGGC CAAGACGCTC ATAGAACATG GCTGTAATCG CCACCACGAG
    GATCACAATG GACGCACAGC CCTGGATCTG GCAGCCTATC AGGGAGCCGC TAGCCTGGTG
    ATCTATATCC TGGAGCAGGG CGGCAATCTG GAACACATTG ATGTTCATGG AATGCGTCCC
    CTGGATCGGG CCATTGCCTG CCGTAACATC CAGGCCGTAC AGGTGTTCCT GCGCAAGGGG
    GCCAAACTGG GACCGACCAC TTGGAGCATG GCCATGGGCA AGCCGGAGAT TCTGGTCATA
    CTACTGAACA AGCTGCTCGA GGACGGTAAT GTGCTGTACC GGAAAAACCG CTTCCAGGAG
    GCTGCTCATC GCTATCAGTA TGCCCTACGC AAGATCTCCG GACTGGAGCA GTTGCTCGAA
    AGGAATGCCA TCTTCGCCCA GCTGCGGACC AATCTTCTGC TGAATCTGTC GCGCTGCAAG
    CGAAAACTGA ATGAACTTGA CGCCTCCATA GATTTGGCCA CGCAAGCGAT TGCCCAGAAG
    CCGCACAGCT ACGAAGGATA CTACGCCAGG GCAAAGGCGC GCATGGAACT TGGTGCATTG
    AATGAAGCAC TTGTGGACGC CAATGAGGCG ATGCAGCAGG CCGCCCAAAG CGGTGTGCTC
    TGCGAAGTGG TCGAGGTGCT GAAGCGCATC CAGACCGAGC TGCTCACGCG GATCAGCATC
    AGCAGCGAGG ATCAGACCGG CAGGATGTCG AATATCGGCG GCGGGGCATC CGTCGCATAC
    GAATCTCATC ATGAGATCAC CGATTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_648542.1
    CDS1044..5753
    Translation

    Target ORF information:

    RefSeq Version NM_140285.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster rolling pebbles (rols), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_140285.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTTCCCAC TAACCAACCG CTCTTCGAGT GGCAATCGCA CCAACAACAA TCCGAGCAAC 
    AACAACAACA ACCAGACCAC AAGCTTCATA ATCGGAGGTA CCACCAATAT TAACAACAAC
    AACAATCAGC TTGGTGGCAC AAACAGCGCT AAGGATAAAG GAAATTTTGA AAATAATTGG
    AGCTGCAATA AAACAACCGG TCGTCGATCT GGCAGTGGAA ATAGTTGCAG TTTTTTAGAT
    CTCCAGTCCA TCCGACGACT CCTGGAGCAC GATGCCAGTG GATCCGTTTG CCCCTCCTGC
    CGCATATCCT TCGACAAGGG CAAGAGACGG AAACTGATCG ACACCTGCGG CCACGAGCGG
    TGCTATTCCT GCATGTTCCG CAACGACCAG TGCCCCATGT GCATGAACAG CTCGCTGAAA
    GATGTTGATG GCGCCAATGC TCAGGGCTAT GACACCGGAA TCGGTGGCTC CACATCGACG
    ATAGTGAGTC CTCTTGGATC CCCGCAGCCG CAGCTCAGAT CGCATGCGCA ACGAAACGCA
    GCGCTGGCGC GCTACATGCA GCAGCACCGC CAGGAGTCGC AGTCGCCAGA TTACCACCAT
    CGCTATGCGA GCAATGGCAA ACTGCCCCGG GCGGCGGCAC TGTCTACCAA TGGCAATGGA
    TTTACCACCG GCACCACGCA AACGGTCACA GTGGATGTGC ATCACCAGCT CGGCGGAGGA
    GGAGGAGGTG CTGCCGCCGC GGCCAAGCAG TTGATGGCCA ACGGCATCAG TGGCCATAGT
    CGCAGCAGCA GCATGAGCCA CAACATCCAT GCGGCAGCCT ACAGCGAACT GAGTGCCGCA
    CCACCGGCCT TCGCCACACC GCCGACGCGT AGGCGGTTCT TTAACCACAA GAATCTGCGC
    AGTGCCCTCA CTGGAGGAAG TGGCGGTGGA TCGGGAGTCG GCGGTGGTGT AGGAGGAGCT
    GGCGGTGTGG GCGGCGGTCA TCGACGCACT GCCTCCAATG GGGGTTGTCC CATTGACACA
    GCATCGATCC TGTCGGGAGG AAGCGACGCT ATTGACCACC ACTCCTCCTT CCTGAATGAG
    AAACTGCAGA AGGATCAGCT CCACGCCCGT CTCGGTCTGC TGCTCAACGA TCCCGGCAGC
    AATGGAAACA GCAGCAGCAG CGGAAGTGGT TGTGAACCCA TCTCGGCCCA TTCCACCACC
    AGCACCACGA GCAGCAGCGG CGTGGGAGCG GCCAGCACCA CCACGAGCGG TTCCTCGCAG
    AATGTCAGTC CGGAGCAAAC GCTGGCCAGC GGAGCCGGAA TGCTCAGTGG CAGTCAGCTG
    TCGGTGGCCA CCAGTCATGG GGTCAAGGAG GATGCACTGA GTCTGTGCGG CAAGTTCAAG
    GCTGGCTGCT CCATGCTGCA CGTCTACGAA GCATTGCCCA GCAAGAGTCG CAAAGGAAAT
    GTACGGAGAT CGACGCGTGG TCAGCAGGGA TCCAGTTCGA GCGCATCGGC GGCATCAGCA
    GTGGGATCCA GGGTGACAGC CTCCAGTTTG GCGGCTGTGC AGTTGGCCCT GAAGCCACTG
    TTCTTTGAGG TGCCGCTGCA GGAACCGGAT CCACCATATG TGGGTCGCCA GTGGCTGGTG
    CAGCAGTTGT CCAACATCCT GCTTGGCACC GAGACACGGG TGGTGCTCAT TAACGGACAG
    CCGGGCACTG GAAAGACGGC GTTTTGTCTG CAGCTGGTGG AGTACTCCTG CTTTGGCCGC
    CGGCAGATGC AGGATGATCC CGATGGCATA TACAGTCAGC TACAGTTGGG CGCCCACTGT
    GAGCGCATGC GCGGACTGGC CTCCCACATG GTTGGCTATC ACTTCTGCCA GGCGGACGCC
    AATCTCACCT GCCAGGTGCC CGATTTCGTG CATTCTCTGG CCGCCCAACT GTGTCAGGCG
    CCGCAGTTAA CCGCCTACAG GGATTACCTG CTCTCGGAGC CGCATCTTCA GGACATTCTC
    AGTGTGCGAG AGTGCATCGC GGATGCGGAG AGAGTAATGA AGCTGGCTAT ACTGGAACCA
    CTGGCTCACC TTCACCGGGC GGGAAAGATA CCCGCCAAAG TGGCCGTCAT CGTGGTGGAT
    GCCTTGTGCG AAGCGGAGTA CCATCGTCCC GATCATGGTC ACACCATTGC TAGTTTCTTG
    GCACAGCTTA CGCCACATTT TCCAGCTTGG CTCAAGCTGG TGGCCACTGT GAGGACCCAG
    ATGTTGGAGC TGGTGAAGGC ACCCAGCTAC ACACAACTTA CCCTGGACAG TTGGGCCAGC
    AGCCAGGCGT TGCAGCAGGA TATGCTTGAT TACATAGGCG CTCGGCTTGC AGATAGTCCG
    GAAATTCGAA TGAACATCGG CGGCGGAGGA GGCCAGAACT CGCAGTCGGG CAGCCAGCCG
    CAGACGAAGT TCGTGTCCCA TCTTCAGTCC CTGAGCAGGG GCTCCATGCT GTACGCTAAG
    CTCATCCTGG ATCTCATTGC ACGCGGCCAG TTGGTTATCA AGTCATCCAG CTACAAGGTG
    CTGCCGGTCT CTCTTGCTCA GATCTTTCTG CTGCACTTCA ACCTGCGCTT TCCCACCGCC
    CGTAGCTTTG AGCAGGCTGC TCCGATCCTC AATATATGCC TGGCTGCTCT GTATCCTCTG
    ACGCTGGACG AGATCTACTA CAGTATGGAG GCACTGAGTC ACGGACGGGA GGCACTAAGC
    TGGCCGGACT TCATGCAACG CTTTAAGCTG CTGGATGGAT TTTTAATCAA GAGGTTGGAC
    AACACGTACA TGTTCTTTCA TAGCTCCTTG AGGGAGTGGC TCATGCGAAG GGATGAGGGG
    GAGTCCAACA AGTTCCTCTG CGACGCCCGT TTGGGTCATG CTGGAATCGC TTTCCGATTA
    TCCCGACTGC AGGCTCCATT GTCACCTCAG CTCACATTGG AACTGGGTCA CCACATGCTG
    AAAGCACATT TATACGGAGG AACTAGTCTG ACGCTGCTTT CTCCGCGGGA TTTGCAATCT
    TATTGGTTGG CGGGAGCGGC GGATAACATT TCATCCTCGC TGGGCGCCCT GCGAAATGTC
    TACAGTCCGA ACCTAAAGGT TTCCCGACTA GTTCTCTTGG CAGGAGCTTC ACCGAATCAC
    CGCACGGATT ACATGGGTGG AGCTCCGATC CTTTGCATAG CTGCGCATGA GGGAATCCTT
    CCCATGGTTA GCCTGCTCCT AGAATTCGGT GCCGATGTCG GTCTGACCAA CAGCCAGGGC
    TGTACTCCAT TAATACTGGC CGCCATGCGT GGCCACTGCG ACGTAGTGCG TCCATTGGTA
    GCCGCCGGCA GCAGCCTCGG TCAGTTGGAC ATCACGCAGC GCTGTGCCCT AGTTCATGCC
    GCCCGCATGG GTCACCTCAG TGTGGTCAAG TACTTGCTCG CCTGCGACTG GTCACCACGA
    CCGCACTCCC AAGATGTCAC CAGATCGGTT GCCTTGCAAC AGGCGCTCAT TGGAGCTGCC
    GCACAGGCGC ACTGCAAGAT CCTTGAGGAT CTTCTGGATC TCAATGAGAC CGAGTTTGAC
    CTGGACGTGA ATGGCATGGA GCCAAGCAGT GGTGAACTGG CGCTAACCGC CGCCGCACGG
    CACGGATGCA TTGATGTTGT AGGAATCTTG CTCTCCCGTG GCGCTCAGAT CGATGCAAGA
    AATCGGCAGG GATACTCGGC GCTTTGGTTG GCTGTCAAGG AGGGTCACTG GAGTGTGGTG
    GAGCATCTGC TGCAACGTGG TGCCCTGTTG GATGAACCAT TGGGACAGAC ACGCAAGACG
    CCGCTAATGA TTGCGGCGGA GGAGGGACAC CTGGAGCTGG TCGATCTGCT GCTGGCGCGG
    GGAGCGCAAC GAGAAGCTCA GGATCACGAG GGATTCACTG CACTCAGCTG GGCCTGTTTA
    CGTGGCCACT TGGCGGCGGC CAAGACGCTC ATAGAACATG GCTGTAATCG CCACCACGAG
    GATCACAATG GACGCACAGC CCTGGATCTG GCAGCCTATC AGGGAGCCGC TAGCCTGGTG
    ATCTATATCC TGGAGCAGGG CGGCAATCTG GAACACATTG ATGTTCATGG AATGCGTCCC
    CTGGATCGGG CCATTGCCTG CCGTAACATC CAGGCCGTAC AGGTGTTCCT GCGCAAGGGG
    GCCAAACTGG GACCGACCAC TTGGAGCATG GCCATGGGCA AGCCGGAGAT TCTGGTCATA
    CTACTGAACA AGCTGCTCGA GGACGGTAAT GTGCTGTACC GGAAAAACCG CTTCCAGGAG
    GCTGCTCATC GCTATCAGTA TGCCCTACGC AAGATCTCCG GACTGGAGCA GTTGCTCGAA
    AGGAATGCCA TCTTCGCCCA GCTGCGGACC AATCTTCTGC TGAATCTGTC GCGCTGCAAG
    CGAAAACTGA ATGAACTTGA CGCCTCCATA GATTTGGCCA CGCAAGCGAT TGCCCAGAAG
    CCGCACAGCT ACGAAGGATA CTACGCCAGG GCAAAGGCGC GCATGGAACT TGGTGCATTG
    AATGAAGCAC TTGTGGACGC CAATGAGGCG ATGCAGCAGG CCGCCCAAAG CGGTGTGCTC
    TGCGAAGTGG TCGAGGTGCT GAAGCGCATC CAGACCGAGC TGCTCACGCG GATCAGCATC
    AGCAGCGAGG ATCAGACCGG CAGGATGTCG AATATCGGCG GCGGGGCATC CGTCGCATAC
    GAATCTCATC ATGAGATCAC CGATTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa18309
    Clone ID Related Accession (Same CDS sequence) NM_140285.3 , NM_168488.2
    Accession Version NM_168488.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product rolling pebbles, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168488.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTTCCCAC TAACCAACCG CTCTTCGAGT GGCAATCGCA CCAACAACAA TCCGAGCAAC 
    AACAACAACA ACCAGACCAC AAGCTTCATA ATCGGAGGTA CCACCAATAT TAACAACAAC
    AACAATCAGC TTGGTGGCAC AAACAGCGCT AAGGATAAAG GAAATTTTGA AAATAATTGG
    AGCTGCAATA AAACAACCGG TCGTCGATCT GGCAGTGGAA ATAGTTGCAG TTTTTTAGAT
    CTCCAGTCCA TCCGACGACT CCTGGAGCAC GATGCCAGTG GATCCGTTTG CCCCTCCTGC
    CGCATATCCT TCGACAAGGG CAAGAGACGG AAACTGATCG ACACCTGCGG CCACGAGCGG
    TGCTATTCCT GCATGTTCCG CAACGACCAG TGCCCCATGT GCATGAACAG CTCGCTGAAA
    GATGTTGATG GCGCCAATGC TCAGGGCTAT GACACCGGAA TCGGTGGCTC CACATCGACG
    ATAGTGAGTC CTCTTGGATC CCCGCAGCCG CAGCTCAGAT CGCATGCGCA ACGAAACGCA
    GCGCTGGCGC GCTACATGCA GCAGCACCGC CAGGAGTCGC AGTCGCCAGA TTACCACCAT
    CGCTATGCGA GCAATGGCAA ACTGCCCCGG GCGGCGGCAC TGTCTACCAA TGGCAATGGA
    TTTACCACCG GCACCACGCA AACGGTCACA GTGGATGTGC ATCACCAGCT CGGCGGAGGA
    GGAGGAGGTG CTGCCGCCGC GGCCAAGCAG TTGATGGCCA ACGGCATCAG TGGCCATAGT
    CGCAGCAGCA GCATGAGCCA CAACATCCAT GCGGCAGCCT ACAGCGAACT GAGTGCCGCA
    CCACCGGCCT TCGCCACACC GCCGACGCGT AGGCGGTTCT TTAACCACAA GAATCTGCGC
    AGTGCCCTCA CTGGAGGAAG TGGCGGTGGA TCGGGAGTCG GCGGTGGTGT AGGAGGAGCT
    GGCGGTGTGG GCGGCGGTCA TCGACGCACT GCCTCCAATG GGGGTTGTCC CATTGACACA
    GCATCGATCC TGTCGGGAGG AAGCGACGCT ATTGACCACC ACTCCTCCTT CCTGAATGAG
    AAACTGCAGA AGGATCAGCT CCACGCCCGT CTCGGTCTGC TGCTCAACGA TCCCGGCAGC
    AATGGAAACA GCAGCAGCAG CGGAAGTGGT TGTGAACCCA TCTCGGCCCA TTCCACCACC
    AGCACCACGA GCAGCAGCGG CGTGGGAGCG GCCAGCACCA CCACGAGCGG TTCCTCGCAG
    AATGTCAGTC CGGAGCAAAC GCTGGCCAGC GGAGCCGGAA TGCTCAGTGG CAGTCAGCTG
    TCGGTGGCCA CCAGTCATGG GGTCAAGGAG GATGCACTGA GTCTGTGCGG CAAGTTCAAG
    GCTGGCTGCT CCATGCTGCA CGTCTACGAA GCATTGCCCA GCAAGAGTCG CAAAGGAAAT
    GTACGGAGAT CGACGCGTGG TCAGCAGGGA TCCAGTTCGA GCGCATCGGC GGCATCAGCA
    GTGGGATCCA GGGTGACAGC CTCCAGTTTG GCGGCTGTGC AGTTGGCCCT GAAGCCACTG
    TTCTTTGAGG TGCCGCTGCA GGAACCGGAT CCACCATATG TGGGTCGCCA GTGGCTGGTG
    CAGCAGTTGT CCAACATCCT GCTTGGCACC GAGACACGGG TGGTGCTCAT TAACGGACAG
    CCGGGCACTG GAAAGACGGC GTTTTGTCTG CAGCTGGTGG AGTACTCCTG CTTTGGCCGC
    CGGCAGATGC AGGATGATCC CGATGGCATA TACAGTCAGC TACAGTTGGG CGCCCACTGT
    GAGCGCATGC GCGGACTGGC CTCCCACATG GTTGGCTATC ACTTCTGCCA GGCGGACGCC
    AATCTCACCT GCCAGGTGCC CGATTTCGTG CATTCTCTGG CCGCCCAACT GTGTCAGGCG
    CCGCAGTTAA CCGCCTACAG GGATTACCTG CTCTCGGAGC CGCATCTTCA GGACATTCTC
    AGTGTGCGAG AGTGCATCGC GGATGCGGAG AGAGTAATGA AGCTGGCTAT ACTGGAACCA
    CTGGCTCACC TTCACCGGGC GGGAAAGATA CCCGCCAAAG TGGCCGTCAT CGTGGTGGAT
    GCCTTGTGCG AAGCGGAGTA CCATCGTCCC GATCATGGTC ACACCATTGC TAGTTTCTTG
    GCACAGCTTA CGCCACATTT TCCAGCTTGG CTCAAGCTGG TGGCCACTGT GAGGACCCAG
    ATGTTGGAGC TGGTGAAGGC ACCCAGCTAC ACACAACTTA CCCTGGACAG TTGGGCCAGC
    AGCCAGGCGT TGCAGCAGGA TATGCTTGAT TACATAGGCG CTCGGCTTGC AGATAGTCCG
    GAAATTCGAA TGAACATCGG CGGCGGAGGA GGCCAGAACT CGCAGTCGGG CAGCCAGCCG
    CAGACGAAGT TCGTGTCCCA TCTTCAGTCC CTGAGCAGGG GCTCCATGCT GTACGCTAAG
    CTCATCCTGG ATCTCATTGC ACGCGGCCAG TTGGTTATCA AGTCATCCAG CTACAAGGTG
    CTGCCGGTCT CTCTTGCTCA GATCTTTCTG CTGCACTTCA ACCTGCGCTT TCCCACCGCC
    CGTAGCTTTG AGCAGGCTGC TCCGATCCTC AATATATGCC TGGCTGCTCT GTATCCTCTG
    ACGCTGGACG AGATCTACTA CAGTATGGAG GCACTGAGTC ACGGACGGGA GGCACTAAGC
    TGGCCGGACT TCATGCAACG CTTTAAGCTG CTGGATGGAT TTTTAATCAA GAGGTTGGAC
    AACACGTACA TGTTCTTTCA TAGCTCCTTG AGGGAGTGGC TCATGCGAAG GGATGAGGGG
    GAGTCCAACA AGTTCCTCTG CGACGCCCGT TTGGGTCATG CTGGAATCGC TTTCCGATTA
    TCCCGACTGC AGGCTCCATT GTCACCTCAG CTCACATTGG AACTGGGTCA CCACATGCTG
    AAAGCACATT TATACGGAGG AACTAGTCTG ACGCTGCTTT CTCCGCGGGA TTTGCAATCT
    TATTGGTTGG CGGGAGCGGC GGATAACATT TCATCCTCGC TGGGCGCCCT GCGAAATGTC
    TACAGTCCGA ACCTAAAGGT TTCCCGACTA GTTCTCTTGG CAGGAGCTTC ACCGAATCAC
    CGCACGGATT ACATGGGTGG AGCTCCGATC CTTTGCATAG CTGCGCATGA GGGAATCCTT
    CCCATGGTTA GCCTGCTCCT AGAATTCGGT GCCGATGTCG GTCTGACCAA CAGCCAGGGC
    TGTACTCCAT TAATACTGGC CGCCATGCGT GGCCACTGCG ACGTAGTGCG TCCATTGGTA
    GCCGCCGGCA GCAGCCTCGG TCAGTTGGAC ATCACGCAGC GCTGTGCCCT AGTTCATGCC
    GCCCGCATGG GTCACCTCAG TGTGGTCAAG TACTTGCTCG CCTGCGACTG GTCACCACGA
    CCGCACTCCC AAGATGTCAC CAGATCGGTT GCCTTGCAAC AGGCGCTCAT TGGAGCTGCC
    GCACAGGCGC ACTGCAAGAT CCTTGAGGAT CTTCTGGATC TCAATGAGAC CGAGTTTGAC
    CTGGACGTGA ATGGCATGGA GCCAAGCAGT GGTGAACTGG CGCTAACCGC CGCCGCACGG
    CACGGATGCA TTGATGTTGT AGGAATCTTG CTCTCCCGTG GCGCTCAGAT CGATGCAAGA
    AATCGGCAGG GATACTCGGC GCTTTGGTTG GCTGTCAAGG AGGGTCACTG GAGTGTGGTG
    GAGCATCTGC TGCAACGTGG TGCCCTGTTG GATGAACCAT TGGGACAGAC ACGCAAGACG
    CCGCTAATGA TTGCGGCGGA GGAGGGACAC CTGGAGCTGG TCGATCTGCT GCTGGCGCGG
    GGAGCGCAAC GAGAAGCTCA GGATCACGAG GGATTCACTG CACTCAGCTG GGCCTGTTTA
    CGTGGCCACT TGGCGGCGGC CAAGACGCTC ATAGAACATG GCTGTAATCG CCACCACGAG
    GATCACAATG GACGCACAGC CCTGGATCTG GCAGCCTATC AGGGAGCCGC TAGCCTGGTG
    ATCTATATCC TGGAGCAGGG CGGCAATCTG GAACACATTG ATGTTCATGG AATGCGTCCC
    CTGGATCGGG CCATTGCCTG CCGTAACATC CAGGCCGTAC AGGTGTTCCT GCGCAAGGGG
    GCCAAACTGG GACCGACCAC TTGGAGCATG GCCATGGGCA AGCCGGAGAT TCTGGTCATA
    CTACTGAACA AGCTGCTCGA GGACGGTAAT GTGCTGTACC GGAAAAACCG CTTCCAGGAG
    GCTGCTCATC GCTATCAGTA TGCCCTACGC AAGATCTCCG GACTGGAGCA GTTGCTCGAA
    AGGAATGCCA TCTTCGCCCA GCTGCGGACC AATCTTCTGC TGAATCTGTC GCGCTGCAAG
    CGAAAACTGA ATGAACTTGA CGCCTCCATA GATTTGGCCA CGCAAGCGAT TGCCCAGAAG
    CCGCACAGCT ACGAAGGATA CTACGCCAGG GCAAAGGCGC GCATGGAACT TGGTGCATTG
    AATGAAGCAC TTGTGGACGC CAATGAGGCG ATGCAGCAGG CCGCCCAAAG CGGTGTGCTC
    TGCGAAGTGG TCGAGGTGCT GAAGCGCATC CAGACCGAGC TGCTCACGCG GATCAGCATC
    AGCAGCGAGG ATCAGACCGG CAGGATGTCG AATATCGGCG GCGGGGCATC CGTCGCATAC
    GAATCTCATC ATGAGATCAC CGATTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_729779.1
    CDS523..5232
    Translation

    Target ORF information:

    RefSeq Version NM_168488.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster rolling pebbles (rols), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_168488.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTTCCCAC TAACCAACCG CTCTTCGAGT GGCAATCGCA CCAACAACAA TCCGAGCAAC 
    AACAACAACA ACCAGACCAC AAGCTTCATA ATCGGAGGTA CCACCAATAT TAACAACAAC
    AACAATCAGC TTGGTGGCAC AAACAGCGCT AAGGATAAAG GAAATTTTGA AAATAATTGG
    AGCTGCAATA AAACAACCGG TCGTCGATCT GGCAGTGGAA ATAGTTGCAG TTTTTTAGAT
    CTCCAGTCCA TCCGACGACT CCTGGAGCAC GATGCCAGTG GATCCGTTTG CCCCTCCTGC
    CGCATATCCT TCGACAAGGG CAAGAGACGG AAACTGATCG ACACCTGCGG CCACGAGCGG
    TGCTATTCCT GCATGTTCCG CAACGACCAG TGCCCCATGT GCATGAACAG CTCGCTGAAA
    GATGTTGATG GCGCCAATGC TCAGGGCTAT GACACCGGAA TCGGTGGCTC CACATCGACG
    ATAGTGAGTC CTCTTGGATC CCCGCAGCCG CAGCTCAGAT CGCATGCGCA ACGAAACGCA
    GCGCTGGCGC GCTACATGCA GCAGCACCGC CAGGAGTCGC AGTCGCCAGA TTACCACCAT
    CGCTATGCGA GCAATGGCAA ACTGCCCCGG GCGGCGGCAC TGTCTACCAA TGGCAATGGA
    TTTACCACCG GCACCACGCA AACGGTCACA GTGGATGTGC ATCACCAGCT CGGCGGAGGA
    GGAGGAGGTG CTGCCGCCGC GGCCAAGCAG TTGATGGCCA ACGGCATCAG TGGCCATAGT
    CGCAGCAGCA GCATGAGCCA CAACATCCAT GCGGCAGCCT ACAGCGAACT GAGTGCCGCA
    CCACCGGCCT TCGCCACACC GCCGACGCGT AGGCGGTTCT TTAACCACAA GAATCTGCGC
    AGTGCCCTCA CTGGAGGAAG TGGCGGTGGA TCGGGAGTCG GCGGTGGTGT AGGAGGAGCT
    GGCGGTGTGG GCGGCGGTCA TCGACGCACT GCCTCCAATG GGGGTTGTCC CATTGACACA
    GCATCGATCC TGTCGGGAGG AAGCGACGCT ATTGACCACC ACTCCTCCTT CCTGAATGAG
    AAACTGCAGA AGGATCAGCT CCACGCCCGT CTCGGTCTGC TGCTCAACGA TCCCGGCAGC
    AATGGAAACA GCAGCAGCAG CGGAAGTGGT TGTGAACCCA TCTCGGCCCA TTCCACCACC
    AGCACCACGA GCAGCAGCGG CGTGGGAGCG GCCAGCACCA CCACGAGCGG TTCCTCGCAG
    AATGTCAGTC CGGAGCAAAC GCTGGCCAGC GGAGCCGGAA TGCTCAGTGG CAGTCAGCTG
    TCGGTGGCCA CCAGTCATGG GGTCAAGGAG GATGCACTGA GTCTGTGCGG CAAGTTCAAG
    GCTGGCTGCT CCATGCTGCA CGTCTACGAA GCATTGCCCA GCAAGAGTCG CAAAGGAAAT
    GTACGGAGAT CGACGCGTGG TCAGCAGGGA TCCAGTTCGA GCGCATCGGC GGCATCAGCA
    GTGGGATCCA GGGTGACAGC CTCCAGTTTG GCGGCTGTGC AGTTGGCCCT GAAGCCACTG
    TTCTTTGAGG TGCCGCTGCA GGAACCGGAT CCACCATATG TGGGTCGCCA GTGGCTGGTG
    CAGCAGTTGT CCAACATCCT GCTTGGCACC GAGACACGGG TGGTGCTCAT TAACGGACAG
    CCGGGCACTG GAAAGACGGC GTTTTGTCTG CAGCTGGTGG AGTACTCCTG CTTTGGCCGC
    CGGCAGATGC AGGATGATCC CGATGGCATA TACAGTCAGC TACAGTTGGG CGCCCACTGT
    GAGCGCATGC GCGGACTGGC CTCCCACATG GTTGGCTATC ACTTCTGCCA GGCGGACGCC
    AATCTCACCT GCCAGGTGCC CGATTTCGTG CATTCTCTGG CCGCCCAACT GTGTCAGGCG
    CCGCAGTTAA CCGCCTACAG GGATTACCTG CTCTCGGAGC CGCATCTTCA GGACATTCTC
    AGTGTGCGAG AGTGCATCGC GGATGCGGAG AGAGTAATGA AGCTGGCTAT ACTGGAACCA
    CTGGCTCACC TTCACCGGGC GGGAAAGATA CCCGCCAAAG TGGCCGTCAT CGTGGTGGAT
    GCCTTGTGCG AAGCGGAGTA CCATCGTCCC GATCATGGTC ACACCATTGC TAGTTTCTTG
    GCACAGCTTA CGCCACATTT TCCAGCTTGG CTCAAGCTGG TGGCCACTGT GAGGACCCAG
    ATGTTGGAGC TGGTGAAGGC ACCCAGCTAC ACACAACTTA CCCTGGACAG TTGGGCCAGC
    AGCCAGGCGT TGCAGCAGGA TATGCTTGAT TACATAGGCG CTCGGCTTGC AGATAGTCCG
    GAAATTCGAA TGAACATCGG CGGCGGAGGA GGCCAGAACT CGCAGTCGGG CAGCCAGCCG
    CAGACGAAGT TCGTGTCCCA TCTTCAGTCC CTGAGCAGGG GCTCCATGCT GTACGCTAAG
    CTCATCCTGG ATCTCATTGC ACGCGGCCAG TTGGTTATCA AGTCATCCAG CTACAAGGTG
    CTGCCGGTCT CTCTTGCTCA GATCTTTCTG CTGCACTTCA ACCTGCGCTT TCCCACCGCC
    CGTAGCTTTG AGCAGGCTGC TCCGATCCTC AATATATGCC TGGCTGCTCT GTATCCTCTG
    ACGCTGGACG AGATCTACTA CAGTATGGAG GCACTGAGTC ACGGACGGGA GGCACTAAGC
    TGGCCGGACT TCATGCAACG CTTTAAGCTG CTGGATGGAT TTTTAATCAA GAGGTTGGAC
    AACACGTACA TGTTCTTTCA TAGCTCCTTG AGGGAGTGGC TCATGCGAAG GGATGAGGGG
    GAGTCCAACA AGTTCCTCTG CGACGCCCGT TTGGGTCATG CTGGAATCGC TTTCCGATTA
    TCCCGACTGC AGGCTCCATT GTCACCTCAG CTCACATTGG AACTGGGTCA CCACATGCTG
    AAAGCACATT TATACGGAGG AACTAGTCTG ACGCTGCTTT CTCCGCGGGA TTTGCAATCT
    TATTGGTTGG CGGGAGCGGC GGATAACATT TCATCCTCGC TGGGCGCCCT GCGAAATGTC
    TACAGTCCGA ACCTAAAGGT TTCCCGACTA GTTCTCTTGG CAGGAGCTTC ACCGAATCAC
    CGCACGGATT ACATGGGTGG AGCTCCGATC CTTTGCATAG CTGCGCATGA GGGAATCCTT
    CCCATGGTTA GCCTGCTCCT AGAATTCGGT GCCGATGTCG GTCTGACCAA CAGCCAGGGC
    TGTACTCCAT TAATACTGGC CGCCATGCGT GGCCACTGCG ACGTAGTGCG TCCATTGGTA
    GCCGCCGGCA GCAGCCTCGG TCAGTTGGAC ATCACGCAGC GCTGTGCCCT AGTTCATGCC
    GCCCGCATGG GTCACCTCAG TGTGGTCAAG TACTTGCTCG CCTGCGACTG GTCACCACGA
    CCGCACTCCC AAGATGTCAC CAGATCGGTT GCCTTGCAAC AGGCGCTCAT TGGAGCTGCC
    GCACAGGCGC ACTGCAAGAT CCTTGAGGAT CTTCTGGATC TCAATGAGAC CGAGTTTGAC
    CTGGACGTGA ATGGCATGGA GCCAAGCAGT GGTGAACTGG CGCTAACCGC CGCCGCACGG
    CACGGATGCA TTGATGTTGT AGGAATCTTG CTCTCCCGTG GCGCTCAGAT CGATGCAAGA
    AATCGGCAGG GATACTCGGC GCTTTGGTTG GCTGTCAAGG AGGGTCACTG GAGTGTGGTG
    GAGCATCTGC TGCAACGTGG TGCCCTGTTG GATGAACCAT TGGGACAGAC ACGCAAGACG
    CCGCTAATGA TTGCGGCGGA GGAGGGACAC CTGGAGCTGG TCGATCTGCT GCTGGCGCGG
    GGAGCGCAAC GAGAAGCTCA GGATCACGAG GGATTCACTG CACTCAGCTG GGCCTGTTTA
    CGTGGCCACT TGGCGGCGGC CAAGACGCTC ATAGAACATG GCTGTAATCG CCACCACGAG
    GATCACAATG GACGCACAGC CCTGGATCTG GCAGCCTATC AGGGAGCCGC TAGCCTGGTG
    ATCTATATCC TGGAGCAGGG CGGCAATCTG GAACACATTG ATGTTCATGG AATGCGTCCC
    CTGGATCGGG CCATTGCCTG CCGTAACATC CAGGCCGTAC AGGTGTTCCT GCGCAAGGGG
    GCCAAACTGG GACCGACCAC TTGGAGCATG GCCATGGGCA AGCCGGAGAT TCTGGTCATA
    CTACTGAACA AGCTGCTCGA GGACGGTAAT GTGCTGTACC GGAAAAACCG CTTCCAGGAG
    GCTGCTCATC GCTATCAGTA TGCCCTACGC AAGATCTCCG GACTGGAGCA GTTGCTCGAA
    AGGAATGCCA TCTTCGCCCA GCTGCGGACC AATCTTCTGC TGAATCTGTC GCGCTGCAAG
    CGAAAACTGA ATGAACTTGA CGCCTCCATA GATTTGGCCA CGCAAGCGAT TGCCCAGAAG
    CCGCACAGCT ACGAAGGATA CTACGCCAGG GCAAAGGCGC GCATGGAACT TGGTGCATTG
    AATGAAGCAC TTGTGGACGC CAATGAGGCG ATGCAGCAGG CCGCCCAAAG CGGTGTGCTC
    TGCGAAGTGG TCGAGGTGCT GAAGCGCATC CAGACCGAGC TGCTCACGCG GATCAGCATC
    AGCAGCGAGG ATCAGACCGG CAGGATGTCG AATATCGGCG GCGGGGCATC CGTCGCATAC
    GAATCTCATC ATGAGATCAC CGATTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa19939
    Clone ID Related Accession (Same CDS sequence) NM_168490.2 , NM_168489.2
    Accession Version NM_168490.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5013bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product rolling pebbles, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168490.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTTCCCAC TAACCAACCG CTCTTCGAGT GGCAATCGCA CCAACAACAA TCCGAGCAAC 
    AACAACAACA ACCAGACCAC AAGCTTCATA ATCGGAGGTA CCACCAATAT TAACAACAAC
    AACAATCAGC TTGGTGGCAC AAACAGCGCT AAGGATAAAG GAAATTTTGA AAATAATTGG
    AGCTGCAATA AAACAACCGG TCGTCGATCT GGCAGTGGAA ATAGTTGCAG TTTTTTAGAT
    CTCCAGTCCA TCCGACGACT CCTGGAGCAC GATGCCAGTG GATCCGTTTG CCCCTCCTGC
    CGCATATCCT TCGACAAGGG CAAGAGACGG AAACTGATCG ACACCTGCGG CCACGAGCGG
    TGCTATTCCT GCATGTTCCG CAACGACCAG TGCCCCATGT GCATGAACAG CTCGCTGAAA
    GATGTTGATG GCGCCAATGC TCAGGGCTAT GACACCGGAA TCGGTGGCTC CACATCGACG
    ATAGTGAGTC CTCTTGGATC CCCGCAGCCG CAGCTCAGAT CGCATGCGCA ACGAAACGCA
    GCGCTGGCGC GCTACATGCA GCAGCACCGC CAGGAGTCGC AGTCGCCAGA TTACCACCAT
    CGCTATGCGA GCAATGGCAA ACTGCCCCGG GCGGCGGCAC TGTCTACCAA TGGCAATGGA
    TTTACCACCG GCACCACGCA AACGGTCACA GTGGATGTGC ATCACCAGCT CGGCGGAGGA
    GGAGGAGGTG CTGCCGCCGC GGCCAAGCAG TTGATGGCCA ACGGCATCAG TGGCCATAGT
    CGCAGCAGCA GCATGAGCCA CAACATCCAT GCGGCAGCCT ACAGCGAACT GAGTGCCGCA
    CCACCGGCCT TCGCCACACC GCCGACGCGT AGGCGGTTCT TTAACCACAA GAATCTGCGC
    AGTGCCCTCA CTGGAGGAAG TGGCGGTGGA TCGGGAGTCG GCGGTGGTGT AGGAGGAGCT
    GGCGGTGTGG GCGGCGGTCA TCGACGCACT GCCTCCAATG GGGGTTGTCC CATTGACACA
    GCATCGATCC TGTCGGGAGA TCATACTCAT CAGCATCATC ACCGCGACAA TCAGCCGGAG
    GGCAAGGAGT CCAATGCCCT TAACACATCC ACCTGCTCGG ACTCGGCGGT CACTAGGAGG
    CGGAGGAAGA ACGTGAGCAA TCACAACCTG AAGACCTCTG CCCGCCATGG AGCAAGTAGC
    GAGAATCGTC TGAACAGGCT CAGTTTGGCC GGAACGTCCG TCTATGCGGG TCATCTGTCC
    TCTTTGGTCT TTGGCAAGAT CAAGTCCCTG TGGAGCGTGA ACTCCTCCAA TTCCAGCGAG
    GCGGGACTGA ATCAATTAGC AGGAAGCGAC GCTATTGACC ACCACTCCTC CTTCCTGAAT
    GAGAAACTGC AGAAGGATCA GCTCCACGCC CGTCTCGGTC TGCTGCTCAA CGATCCCGGC
    AGCAATGGAA ACAGCAGCAG CAGCGGAAGT GGTTGTGAAC CCATCTCGGC CCATTCCACC
    ACCAGCACCA CGAGCAGCAG CGGCGTGGGA GCGGCCAGCA CCACCACGAG CGGTTCCTCG
    CAGAATGTCA GTCCGGAGCA AACGCTGGCC AGCGGAGCCG GAATGCTCAG TGGCAGTCAG
    CTGTCGGTGG CCACCAGTCA TGGGGTCAAG GAGGATGCAC TGAGTCTGTG CGGCAAGTTC
    AAGGCTGGCT GCTCCATGCT GCACGTCTAC GAAGCATTGC CCAGCAAGAG TCGCAAAGGA
    AATGTACGGA GATCGACGCG TGGTCAGCAG GGATCCAGTT CGAGCGCATC GGCGGCATCA
    GCAGTGGGAT CCAGGGTGAC AGCCTCCAGT TTGGCGGCTG TGCAGTTGGC CCTGAAGCCA
    CTGTTCTTTG AGGTGCCGCT GCAGGAACCG GATCCACCAT ATGTGGGTCG CCAGTGGCTG
    GTGCAGCAGT TGTCCAACAT CCTGCTTGGC ACCGAGACAC GGGTGGTGCT CATTAACGGA
    CAGCCGGGCA CTGGAAAGAC GGCGTTTTGT CTGCAGCTGG TGGAGTACTC CTGCTTTGGC
    CGCCGGCAGA TGCAGGATGA TCCCGATGGC ATATACAGTC AGCTACAGTT GGGCGCCCAC
    TGTGAGCGCA TGCGCGGACT GGCCTCCCAC ATGGTTGGCT ATCACTTCTG CCAGGCGGAC
    GCCAATCTCA CCTGCCAGGT GCCCGATTTC GTGCATTCTC TGGCCGCCCA ACTGTGTCAG
    GCGCCGCAGT TAACCGCCTA CAGGGATTAC CTGCTCTCGG AGCCGCATCT TCAGGACATT
    CTCAGTGTGC GAGAGTGCAT CGCGGATGCG GAGAGAGTAA TGAAGCTGGC TATACTGGAA
    CCACTGGCTC ACCTTCACCG GGCGGGAAAG ATACCCGCCA AAGTGGCCGT CATCGTGGTG
    GATGCCTTGT GCGAAGCGGA GTACCATCGT CCCGATCATG GTCACACCAT TGCTAGTTTC
    TTGGCACAGC TTACGCCACA TTTTCCAGCT TGGCTCAAGC TGGTGGCCAC TGTGAGGACC
    CAGATGTTGG AGCTGGTGAA GGCACCCAGC TACACACAAC TTACCCTGGA CAGTTGGGCC
    AGCAGCCAGG CGTTGCAGCA GGATATGCTT GATTACATAG GCGCTCGGCT TGCAGATAGT
    CCGGAAATTC GAATGAACAT CGGCGGCGGA GGAGGCCAGA ACTCGCAGTC GGGCAGCCAG
    CCGCAGACGA AGTTCGTGTC CCATCTTCAG TCCCTGAGCA GGGGCTCCAT GCTGTACGCT
    AAGCTCATCC TGGATCTCAT TGCACGCGGC CAGTTGGTTA TCAAGTCATC CAGCTACAAG
    GTGCTGCCGG TCTCTCTTGC TCAGATCTTT CTGCTGCACT TCAACCTGCG CTTTCCCACC
    GCCCGTAGCT TTGAGCAGGC TGCTCCGATC CTCAATATAT GCCTGGCTGC TCTGTATCCT
    CTGACGCTGG ACGAGATCTA CTACAGTATG GAGGCACTGA GTCACGGACG GGAGGCACTA
    AGCTGGCCGG ACTTCATGCA ACGCTTTAAG CTGCTGGATG GATTTTTAAT CAAGAGGTTG
    GACAACACGT ACATGTTCTT TCATAGCTCC TTGAGGGAGT GGCTCATGCG AAGGGATGAG
    GGGGAGTCCA ACAAGTTCCT CTGCGACGCC CGTTTGGGTC ATGCTGGAAT CGCTTTCCGA
    TTATCCCGAC TGCAGGCTCC ATTGTCACCT CAGCTCACAT TGGAACTGGG TCACCACATG
    CTGAAAGCAC ATTTATACGG AGGAACTAGT CTGACGCTGC TTTCTCCGCG GGATTTGCAA
    TCTTATTGGT TGGCGGGAGC GGCGGATAAC ATTTCATCCT CGCTGGGCGC CCTGCGAAAT
    GTCTACAGTC CGAACCTAAA GGTTTCCCGA CTAGTTCTCT TGGCAGGAGC TTCACCGAAT
    CACCGCACGG ATTACATGGG TGGAGCTCCG ATCCTTTGCA TAGCTGCGCA TGAGGGAATC
    CTTCCCATGG TTAGCCTGCT CCTAGAATTC GGTGCCGATG TCGGTCTGAC CAACAGCCAG
    GGCTGTACTC CATTAATACT GGCCGCCATG CGTGGCCACT GCGACGTAGT GCGTCCATTG
    GTAGCCGCCG GCAGCAGCCT CGGTCAGTTG GACATCACGC AGCGCTGTGC CCTAGTTCAT
    GCCGCCCGCA TGGGTCACCT CAGTGTGGTC AAGTACTTGC TCGCCTGCGA CTGGTCACCA
    CGACCGCACT CCCAAGATGT CACCAGATCG GTTGCCTTGC AACAGGCGCT CATTGGAGCT
    GCCGCACAGG CGCACTGCAA GATCCTTGAG GATCTTCTGG ATCTCAATGA GACCGAGTTT
    GACCTGGACG TGAATGGCAT GGAGCCAAGC AGTGGTGAAC TGGCGCTAAC CGCCGCCGCA
    CGGCACGGAT GCATTGATGT TGTAGGAATC TTGCTCTCCC GTGGCGCTCA GATCGATGCA
    AGAAATCGGC AGGGATACTC GGCGCTTTGG TTGGCTGTCA AGGAGGGTCA CTGGAGTGTG
    GTGGAGCATC TGCTGCAACG TGGTGCCCTG TTGGATGAAC CATTGGGACA GACACGCAAG
    ACGCCGCTAA TGATTGCGGC GGAGGAGGGA CACCTGGAGC TGGTCGATCT GCTGCTGGCG
    CGGGGAGCGC AACGAGAAGC TCAGGATCAC GAGGGATTCA CTGCACTCAG CTGGGCCTGT
    TTACGTGGCC ACTTGGCGGC GGCCAAGACG CTCATAGAAC ATGGCTGTAA TCGCCACCAC
    GAGGATCACA ATGGACGCAC AGCCCTGGAT CTGGCAGCCT ATCAGGGAGC CGCTAGCCTG
    GTGATCTATA TCCTGGAGCA GGGCGGCAAT CTGGAACACA TTGATGTTCA TGGAATGCGT
    CCCCTGGATC GGGCCATTGC CTGCCGTAAC ATCCAGGCCG TACAGGTGTT CCTGCGCAAG
    GGGGCCAAAC TGGGACCGAC CACTTGGAGC ATGGCCATGG GCAAGCCGGA GATTCTGGTC
    ATACTACTGA ACAAGCTGCT CGAGGACGGT AATGTGCTGT ACCGGAAAAA CCGCTTCCAG
    GAGGCTGCTC ATCGCTATCA GTATGCCCTA CGCAAGATCT CCGGACTGGA GCAGTTGCTC
    GAAAGGAATG CCATCTTCGC CCAGCTGCGG ACCAATCTTC TGCTGAATCT GTCGCGCTGC
    AAGCGAAAAC TGAATGAACT TGACGCCTCC ATAGATTTGG CCACGCAAGC GATTGCCCAG
    AAGCCGCACA GCTACGAAGG ATACTACGCC AGGGCAAAGG CGCGCATGGA ACTTGGTGCA
    TTGAATGAAG CACTTGTGGA CGCCAATGAG GCGATGCAGC AGGCCGCCCA AAGCGGTGTG
    CTCTGCGAAG TGGTCGAGGT GCTGAAGCGC ATCCAGACCG AGCTGCTCAC GCGGATCAGC
    ATCAGCAGCG AGGATCAGAC CGGCAGGATG TCGAATATCG GCGGCGGGGC ATCCGTCGCA
    TACGAATCTC ATCATGAGAT CACCGATTTG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_729781.1
    CDS564..5576
    Translation

    Target ORF information:

    RefSeq Version NM_168490.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster rolling pebbles (rols), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_168490.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTTCCCAC TAACCAACCG CTCTTCGAGT GGCAATCGCA CCAACAACAA TCCGAGCAAC 
    AACAACAACA ACCAGACCAC AAGCTTCATA ATCGGAGGTA CCACCAATAT TAACAACAAC
    AACAATCAGC TTGGTGGCAC AAACAGCGCT AAGGATAAAG GAAATTTTGA AAATAATTGG
    AGCTGCAATA AAACAACCGG TCGTCGATCT GGCAGTGGAA ATAGTTGCAG TTTTTTAGAT
    CTCCAGTCCA TCCGACGACT CCTGGAGCAC GATGCCAGTG GATCCGTTTG CCCCTCCTGC
    CGCATATCCT TCGACAAGGG CAAGAGACGG AAACTGATCG ACACCTGCGG CCACGAGCGG
    TGCTATTCCT GCATGTTCCG CAACGACCAG TGCCCCATGT GCATGAACAG CTCGCTGAAA
    GATGTTGATG GCGCCAATGC TCAGGGCTAT GACACCGGAA TCGGTGGCTC CACATCGACG
    ATAGTGAGTC CTCTTGGATC CCCGCAGCCG CAGCTCAGAT CGCATGCGCA ACGAAACGCA
    GCGCTGGCGC GCTACATGCA GCAGCACCGC CAGGAGTCGC AGTCGCCAGA TTACCACCAT
    CGCTATGCGA GCAATGGCAA ACTGCCCCGG GCGGCGGCAC TGTCTACCAA TGGCAATGGA
    TTTACCACCG GCACCACGCA AACGGTCACA GTGGATGTGC ATCACCAGCT CGGCGGAGGA
    GGAGGAGGTG CTGCCGCCGC GGCCAAGCAG TTGATGGCCA ACGGCATCAG TGGCCATAGT
    CGCAGCAGCA GCATGAGCCA CAACATCCAT GCGGCAGCCT ACAGCGAACT GAGTGCCGCA
    CCACCGGCCT TCGCCACACC GCCGACGCGT AGGCGGTTCT TTAACCACAA GAATCTGCGC
    AGTGCCCTCA CTGGAGGAAG TGGCGGTGGA TCGGGAGTCG GCGGTGGTGT AGGAGGAGCT
    GGCGGTGTGG GCGGCGGTCA TCGACGCACT GCCTCCAATG GGGGTTGTCC CATTGACACA
    GCATCGATCC TGTCGGGAGA TCATACTCAT CAGCATCATC ACCGCGACAA TCAGCCGGAG
    GGCAAGGAGT CCAATGCCCT TAACACATCC ACCTGCTCGG ACTCGGCGGT CACTAGGAGG
    CGGAGGAAGA ACGTGAGCAA TCACAACCTG AAGACCTCTG CCCGCCATGG AGCAAGTAGC
    GAGAATCGTC TGAACAGGCT CAGTTTGGCC GGAACGTCCG TCTATGCGGG TCATCTGTCC
    TCTTTGGTCT TTGGCAAGAT CAAGTCCCTG TGGAGCGTGA ACTCCTCCAA TTCCAGCGAG
    GCGGGACTGA ATCAATTAGC AGGAAGCGAC GCTATTGACC ACCACTCCTC CTTCCTGAAT
    GAGAAACTGC AGAAGGATCA GCTCCACGCC CGTCTCGGTC TGCTGCTCAA CGATCCCGGC
    AGCAATGGAA ACAGCAGCAG CAGCGGAAGT GGTTGTGAAC CCATCTCGGC CCATTCCACC
    ACCAGCACCA CGAGCAGCAG CGGCGTGGGA GCGGCCAGCA CCACCACGAG CGGTTCCTCG
    CAGAATGTCA GTCCGGAGCA AACGCTGGCC AGCGGAGCCG GAATGCTCAG TGGCAGTCAG
    CTGTCGGTGG CCACCAGTCA TGGGGTCAAG GAGGATGCAC TGAGTCTGTG CGGCAAGTTC
    AAGGCTGGCT GCTCCATGCT GCACGTCTAC GAAGCATTGC CCAGCAAGAG TCGCAAAGGA
    AATGTACGGA GATCGACGCG TGGTCAGCAG GGATCCAGTT CGAGCGCATC GGCGGCATCA
    GCAGTGGGAT CCAGGGTGAC AGCCTCCAGT TTGGCGGCTG TGCAGTTGGC CCTGAAGCCA
    CTGTTCTTTG AGGTGCCGCT GCAGGAACCG GATCCACCAT ATGTGGGTCG CCAGTGGCTG
    GTGCAGCAGT TGTCCAACAT CCTGCTTGGC ACCGAGACAC GGGTGGTGCT CATTAACGGA
    CAGCCGGGCA CTGGAAAGAC GGCGTTTTGT CTGCAGCTGG TGGAGTACTC CTGCTTTGGC
    CGCCGGCAGA TGCAGGATGA TCCCGATGGC ATATACAGTC AGCTACAGTT GGGCGCCCAC
    TGTGAGCGCA TGCGCGGACT GGCCTCCCAC ATGGTTGGCT ATCACTTCTG CCAGGCGGAC
    GCCAATCTCA CCTGCCAGGT GCCCGATTTC GTGCATTCTC TGGCCGCCCA ACTGTGTCAG
    GCGCCGCAGT TAACCGCCTA CAGGGATTAC CTGCTCTCGG AGCCGCATCT TCAGGACATT
    CTCAGTGTGC GAGAGTGCAT CGCGGATGCG GAGAGAGTAA TGAAGCTGGC TATACTGGAA
    CCACTGGCTC ACCTTCACCG GGCGGGAAAG ATACCCGCCA AAGTGGCCGT CATCGTGGTG
    GATGCCTTGT GCGAAGCGGA GTACCATCGT CCCGATCATG GTCACACCAT TGCTAGTTTC
    TTGGCACAGC TTACGCCACA TTTTCCAGCT TGGCTCAAGC TGGTGGCCAC TGTGAGGACC
    CAGATGTTGG AGCTGGTGAA GGCACCCAGC TACACACAAC TTACCCTGGA CAGTTGGGCC
    AGCAGCCAGG CGTTGCAGCA GGATATGCTT GATTACATAG GCGCTCGGCT TGCAGATAGT
    CCGGAAATTC GAATGAACAT CGGCGGCGGA GGAGGCCAGA ACTCGCAGTC GGGCAGCCAG
    CCGCAGACGA AGTTCGTGTC CCATCTTCAG TCCCTGAGCA GGGGCTCCAT GCTGTACGCT
    AAGCTCATCC TGGATCTCAT TGCACGCGGC CAGTTGGTTA TCAAGTCATC CAGCTACAAG
    GTGCTGCCGG TCTCTCTTGC TCAGATCTTT CTGCTGCACT TCAACCTGCG CTTTCCCACC
    GCCCGTAGCT TTGAGCAGGC TGCTCCGATC CTCAATATAT GCCTGGCTGC TCTGTATCCT
    CTGACGCTGG ACGAGATCTA CTACAGTATG GAGGCACTGA GTCACGGACG GGAGGCACTA
    AGCTGGCCGG ACTTCATGCA ACGCTTTAAG CTGCTGGATG GATTTTTAAT CAAGAGGTTG
    GACAACACGT ACATGTTCTT TCATAGCTCC TTGAGGGAGT GGCTCATGCG AAGGGATGAG
    GGGGAGTCCA ACAAGTTCCT CTGCGACGCC CGTTTGGGTC ATGCTGGAAT CGCTTTCCGA
    TTATCCCGAC TGCAGGCTCC ATTGTCACCT CAGCTCACAT TGGAACTGGG TCACCACATG
    CTGAAAGCAC ATTTATACGG AGGAACTAGT CTGACGCTGC TTTCTCCGCG GGATTTGCAA
    TCTTATTGGT TGGCGGGAGC GGCGGATAAC ATTTCATCCT CGCTGGGCGC CCTGCGAAAT
    GTCTACAGTC CGAACCTAAA GGTTTCCCGA CTAGTTCTCT TGGCAGGAGC TTCACCGAAT
    CACCGCACGG ATTACATGGG TGGAGCTCCG ATCCTTTGCA TAGCTGCGCA TGAGGGAATC
    CTTCCCATGG TTAGCCTGCT CCTAGAATTC GGTGCCGATG TCGGTCTGAC CAACAGCCAG
    GGCTGTACTC CATTAATACT GGCCGCCATG CGTGGCCACT GCGACGTAGT GCGTCCATTG
    GTAGCCGCCG GCAGCAGCCT CGGTCAGTTG GACATCACGC AGCGCTGTGC CCTAGTTCAT
    GCCGCCCGCA TGGGTCACCT CAGTGTGGTC AAGTACTTGC TCGCCTGCGA CTGGTCACCA
    CGACCGCACT CCCAAGATGT CACCAGATCG GTTGCCTTGC AACAGGCGCT CATTGGAGCT
    GCCGCACAGG CGCACTGCAA GATCCTTGAG GATCTTCTGG ATCTCAATGA GACCGAGTTT
    GACCTGGACG TGAATGGCAT GGAGCCAAGC AGTGGTGAAC TGGCGCTAAC CGCCGCCGCA
    CGGCACGGAT GCATTGATGT TGTAGGAATC TTGCTCTCCC GTGGCGCTCA GATCGATGCA
    AGAAATCGGC AGGGATACTC GGCGCTTTGG TTGGCTGTCA AGGAGGGTCA CTGGAGTGTG
    GTGGAGCATC TGCTGCAACG TGGTGCCCTG TTGGATGAAC CATTGGGACA GACACGCAAG
    ACGCCGCTAA TGATTGCGGC GGAGGAGGGA CACCTGGAGC TGGTCGATCT GCTGCTGGCG
    CGGGGAGCGC AACGAGAAGC TCAGGATCAC GAGGGATTCA CTGCACTCAG CTGGGCCTGT
    TTACGTGGCC ACTTGGCGGC GGCCAAGACG CTCATAGAAC ATGGCTGTAA TCGCCACCAC
    GAGGATCACA ATGGACGCAC AGCCCTGGAT CTGGCAGCCT ATCAGGGAGC CGCTAGCCTG
    GTGATCTATA TCCTGGAGCA GGGCGGCAAT CTGGAACACA TTGATGTTCA TGGAATGCGT
    CCCCTGGATC GGGCCATTGC CTGCCGTAAC ATCCAGGCCG TACAGGTGTT CCTGCGCAAG
    GGGGCCAAAC TGGGACCGAC CACTTGGAGC ATGGCCATGG GCAAGCCGGA GATTCTGGTC
    ATACTACTGA ACAAGCTGCT CGAGGACGGT AATGTGCTGT ACCGGAAAAA CCGCTTCCAG
    GAGGCTGCTC ATCGCTATCA GTATGCCCTA CGCAAGATCT CCGGACTGGA GCAGTTGCTC
    GAAAGGAATG CCATCTTCGC CCAGCTGCGG ACCAATCTTC TGCTGAATCT GTCGCGCTGC
    AAGCGAAAAC TGAATGAACT TGACGCCTCC ATAGATTTGG CCACGCAAGC GATTGCCCAG
    AAGCCGCACA GCTACGAAGG ATACTACGCC AGGGCAAAGG CGCGCATGGA ACTTGGTGCA
    TTGAATGAAG CACTTGTGGA CGCCAATGAG GCGATGCAGC AGGCCGCCCA AAGCGGTGTG
    CTCTGCGAAG TGGTCGAGGT GCTGAAGCGC ATCCAGACCG AGCTGCTCAC GCGGATCAGC
    ATCAGCAGCG AGGATCAGAC CGGCAGGATG TCGAATATCG GCGGCGGGGC ATCCGTCGCA
    TACGAATCTC ATCATGAGAT CACCGATTTG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa19939
    Clone ID Related Accession (Same CDS sequence) NM_168490.2 , NM_168489.2
    Accession Version NM_168489.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5013bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product rolling pebbles, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168489.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTTCCCAC TAACCAACCG CTCTTCGAGT GGCAATCGCA CCAACAACAA TCCGAGCAAC 
    AACAACAACA ACCAGACCAC AAGCTTCATA ATCGGAGGTA CCACCAATAT TAACAACAAC
    AACAATCAGC TTGGTGGCAC AAACAGCGCT AAGGATAAAG GAAATTTTGA AAATAATTGG
    AGCTGCAATA AAACAACCGG TCGTCGATCT GGCAGTGGAA ATAGTTGCAG TTTTTTAGAT
    CTCCAGTCCA TCCGACGACT CCTGGAGCAC GATGCCAGTG GATCCGTTTG CCCCTCCTGC
    CGCATATCCT TCGACAAGGG CAAGAGACGG AAACTGATCG ACACCTGCGG CCACGAGCGG
    TGCTATTCCT GCATGTTCCG CAACGACCAG TGCCCCATGT GCATGAACAG CTCGCTGAAA
    GATGTTGATG GCGCCAATGC TCAGGGCTAT GACACCGGAA TCGGTGGCTC CACATCGACG
    ATAGTGAGTC CTCTTGGATC CCCGCAGCCG CAGCTCAGAT CGCATGCGCA ACGAAACGCA
    GCGCTGGCGC GCTACATGCA GCAGCACCGC CAGGAGTCGC AGTCGCCAGA TTACCACCAT
    CGCTATGCGA GCAATGGCAA ACTGCCCCGG GCGGCGGCAC TGTCTACCAA TGGCAATGGA
    TTTACCACCG GCACCACGCA AACGGTCACA GTGGATGTGC ATCACCAGCT CGGCGGAGGA
    GGAGGAGGTG CTGCCGCCGC GGCCAAGCAG TTGATGGCCA ACGGCATCAG TGGCCATAGT
    CGCAGCAGCA GCATGAGCCA CAACATCCAT GCGGCAGCCT ACAGCGAACT GAGTGCCGCA
    CCACCGGCCT TCGCCACACC GCCGACGCGT AGGCGGTTCT TTAACCACAA GAATCTGCGC
    AGTGCCCTCA CTGGAGGAAG TGGCGGTGGA TCGGGAGTCG GCGGTGGTGT AGGAGGAGCT
    GGCGGTGTGG GCGGCGGTCA TCGACGCACT GCCTCCAATG GGGGTTGTCC CATTGACACA
    GCATCGATCC TGTCGGGAGA TCATACTCAT CAGCATCATC ACCGCGACAA TCAGCCGGAG
    GGCAAGGAGT CCAATGCCCT TAACACATCC ACCTGCTCGG ACTCGGCGGT CACTAGGAGG
    CGGAGGAAGA ACGTGAGCAA TCACAACCTG AAGACCTCTG CCCGCCATGG AGCAAGTAGC
    GAGAATCGTC TGAACAGGCT CAGTTTGGCC GGAACGTCCG TCTATGCGGG TCATCTGTCC
    TCTTTGGTCT TTGGCAAGAT CAAGTCCCTG TGGAGCGTGA ACTCCTCCAA TTCCAGCGAG
    GCGGGACTGA ATCAATTAGC AGGAAGCGAC GCTATTGACC ACCACTCCTC CTTCCTGAAT
    GAGAAACTGC AGAAGGATCA GCTCCACGCC CGTCTCGGTC TGCTGCTCAA CGATCCCGGC
    AGCAATGGAA ACAGCAGCAG CAGCGGAAGT GGTTGTGAAC CCATCTCGGC CCATTCCACC
    ACCAGCACCA CGAGCAGCAG CGGCGTGGGA GCGGCCAGCA CCACCACGAG CGGTTCCTCG
    CAGAATGTCA GTCCGGAGCA AACGCTGGCC AGCGGAGCCG GAATGCTCAG TGGCAGTCAG
    CTGTCGGTGG CCACCAGTCA TGGGGTCAAG GAGGATGCAC TGAGTCTGTG CGGCAAGTTC
    AAGGCTGGCT GCTCCATGCT GCACGTCTAC GAAGCATTGC CCAGCAAGAG TCGCAAAGGA
    AATGTACGGA GATCGACGCG TGGTCAGCAG GGATCCAGTT CGAGCGCATC GGCGGCATCA
    GCAGTGGGAT CCAGGGTGAC AGCCTCCAGT TTGGCGGCTG TGCAGTTGGC CCTGAAGCCA
    CTGTTCTTTG AGGTGCCGCT GCAGGAACCG GATCCACCAT ATGTGGGTCG CCAGTGGCTG
    GTGCAGCAGT TGTCCAACAT CCTGCTTGGC ACCGAGACAC GGGTGGTGCT CATTAACGGA
    CAGCCGGGCA CTGGAAAGAC GGCGTTTTGT CTGCAGCTGG TGGAGTACTC CTGCTTTGGC
    CGCCGGCAGA TGCAGGATGA TCCCGATGGC ATATACAGTC AGCTACAGTT GGGCGCCCAC
    TGTGAGCGCA TGCGCGGACT GGCCTCCCAC ATGGTTGGCT ATCACTTCTG CCAGGCGGAC
    GCCAATCTCA CCTGCCAGGT GCCCGATTTC GTGCATTCTC TGGCCGCCCA ACTGTGTCAG
    GCGCCGCAGT TAACCGCCTA CAGGGATTAC CTGCTCTCGG AGCCGCATCT TCAGGACATT
    CTCAGTGTGC GAGAGTGCAT CGCGGATGCG GAGAGAGTAA TGAAGCTGGC TATACTGGAA
    CCACTGGCTC ACCTTCACCG GGCGGGAAAG ATACCCGCCA AAGTGGCCGT CATCGTGGTG
    GATGCCTTGT GCGAAGCGGA GTACCATCGT CCCGATCATG GTCACACCAT TGCTAGTTTC
    TTGGCACAGC TTACGCCACA TTTTCCAGCT TGGCTCAAGC TGGTGGCCAC TGTGAGGACC
    CAGATGTTGG AGCTGGTGAA GGCACCCAGC TACACACAAC TTACCCTGGA CAGTTGGGCC
    AGCAGCCAGG CGTTGCAGCA GGATATGCTT GATTACATAG GCGCTCGGCT TGCAGATAGT
    CCGGAAATTC GAATGAACAT CGGCGGCGGA GGAGGCCAGA ACTCGCAGTC GGGCAGCCAG
    CCGCAGACGA AGTTCGTGTC CCATCTTCAG TCCCTGAGCA GGGGCTCCAT GCTGTACGCT
    AAGCTCATCC TGGATCTCAT TGCACGCGGC CAGTTGGTTA TCAAGTCATC CAGCTACAAG
    GTGCTGCCGG TCTCTCTTGC TCAGATCTTT CTGCTGCACT TCAACCTGCG CTTTCCCACC
    GCCCGTAGCT TTGAGCAGGC TGCTCCGATC CTCAATATAT GCCTGGCTGC TCTGTATCCT
    CTGACGCTGG ACGAGATCTA CTACAGTATG GAGGCACTGA GTCACGGACG GGAGGCACTA
    AGCTGGCCGG ACTTCATGCA ACGCTTTAAG CTGCTGGATG GATTTTTAAT CAAGAGGTTG
    GACAACACGT ACATGTTCTT TCATAGCTCC TTGAGGGAGT GGCTCATGCG AAGGGATGAG
    GGGGAGTCCA ACAAGTTCCT CTGCGACGCC CGTTTGGGTC ATGCTGGAAT CGCTTTCCGA
    TTATCCCGAC TGCAGGCTCC ATTGTCACCT CAGCTCACAT TGGAACTGGG TCACCACATG
    CTGAAAGCAC ATTTATACGG AGGAACTAGT CTGACGCTGC TTTCTCCGCG GGATTTGCAA
    TCTTATTGGT TGGCGGGAGC GGCGGATAAC ATTTCATCCT CGCTGGGCGC CCTGCGAAAT
    GTCTACAGTC CGAACCTAAA GGTTTCCCGA CTAGTTCTCT TGGCAGGAGC TTCACCGAAT
    CACCGCACGG ATTACATGGG TGGAGCTCCG ATCCTTTGCA TAGCTGCGCA TGAGGGAATC
    CTTCCCATGG TTAGCCTGCT CCTAGAATTC GGTGCCGATG TCGGTCTGAC CAACAGCCAG
    GGCTGTACTC CATTAATACT GGCCGCCATG CGTGGCCACT GCGACGTAGT GCGTCCATTG
    GTAGCCGCCG GCAGCAGCCT CGGTCAGTTG GACATCACGC AGCGCTGTGC CCTAGTTCAT
    GCCGCCCGCA TGGGTCACCT CAGTGTGGTC AAGTACTTGC TCGCCTGCGA CTGGTCACCA
    CGACCGCACT CCCAAGATGT CACCAGATCG GTTGCCTTGC AACAGGCGCT CATTGGAGCT
    GCCGCACAGG CGCACTGCAA GATCCTTGAG GATCTTCTGG ATCTCAATGA GACCGAGTTT
    GACCTGGACG TGAATGGCAT GGAGCCAAGC AGTGGTGAAC TGGCGCTAAC CGCCGCCGCA
    CGGCACGGAT GCATTGATGT TGTAGGAATC TTGCTCTCCC GTGGCGCTCA GATCGATGCA
    AGAAATCGGC AGGGATACTC GGCGCTTTGG TTGGCTGTCA AGGAGGGTCA CTGGAGTGTG
    GTGGAGCATC TGCTGCAACG TGGTGCCCTG TTGGATGAAC CATTGGGACA GACACGCAAG
    ACGCCGCTAA TGATTGCGGC GGAGGAGGGA CACCTGGAGC TGGTCGATCT GCTGCTGGCG
    CGGGGAGCGC AACGAGAAGC TCAGGATCAC GAGGGATTCA CTGCACTCAG CTGGGCCTGT
    TTACGTGGCC ACTTGGCGGC GGCCAAGACG CTCATAGAAC ATGGCTGTAA TCGCCACCAC
    GAGGATCACA ATGGACGCAC AGCCCTGGAT CTGGCAGCCT ATCAGGGAGC CGCTAGCCTG
    GTGATCTATA TCCTGGAGCA GGGCGGCAAT CTGGAACACA TTGATGTTCA TGGAATGCGT
    CCCCTGGATC GGGCCATTGC CTGCCGTAAC ATCCAGGCCG TACAGGTGTT CCTGCGCAAG
    GGGGCCAAAC TGGGACCGAC CACTTGGAGC ATGGCCATGG GCAAGCCGGA GATTCTGGTC
    ATACTACTGA ACAAGCTGCT CGAGGACGGT AATGTGCTGT ACCGGAAAAA CCGCTTCCAG
    GAGGCTGCTC ATCGCTATCA GTATGCCCTA CGCAAGATCT CCGGACTGGA GCAGTTGCTC
    GAAAGGAATG CCATCTTCGC CCAGCTGCGG ACCAATCTTC TGCTGAATCT GTCGCGCTGC
    AAGCGAAAAC TGAATGAACT TGACGCCTCC ATAGATTTGG CCACGCAAGC GATTGCCCAG
    AAGCCGCACA GCTACGAAGG ATACTACGCC AGGGCAAAGG CGCGCATGGA ACTTGGTGCA
    TTGAATGAAG CACTTGTGGA CGCCAATGAG GCGATGCAGC AGGCCGCCCA AAGCGGTGTG
    CTCTGCGAAG TGGTCGAGGT GCTGAAGCGC ATCCAGACCG AGCTGCTCAC GCGGATCAGC
    ATCAGCAGCG AGGATCAGAC CGGCAGGATG TCGAATATCG GCGGCGGGGC ATCCGTCGCA
    TACGAATCTC ATCATGAGAT CACCGATTTG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_729780.1
    CDS547..5559
    Translation

    Target ORF information:

    RefSeq Version NM_168489.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster rolling pebbles (rols), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_168489.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTTCCCAC TAACCAACCG CTCTTCGAGT GGCAATCGCA CCAACAACAA TCCGAGCAAC 
    AACAACAACA ACCAGACCAC AAGCTTCATA ATCGGAGGTA CCACCAATAT TAACAACAAC
    AACAATCAGC TTGGTGGCAC AAACAGCGCT AAGGATAAAG GAAATTTTGA AAATAATTGG
    AGCTGCAATA AAACAACCGG TCGTCGATCT GGCAGTGGAA ATAGTTGCAG TTTTTTAGAT
    CTCCAGTCCA TCCGACGACT CCTGGAGCAC GATGCCAGTG GATCCGTTTG CCCCTCCTGC
    CGCATATCCT TCGACAAGGG CAAGAGACGG AAACTGATCG ACACCTGCGG CCACGAGCGG
    TGCTATTCCT GCATGTTCCG CAACGACCAG TGCCCCATGT GCATGAACAG CTCGCTGAAA
    GATGTTGATG GCGCCAATGC TCAGGGCTAT GACACCGGAA TCGGTGGCTC CACATCGACG
    ATAGTGAGTC CTCTTGGATC CCCGCAGCCG CAGCTCAGAT CGCATGCGCA ACGAAACGCA
    GCGCTGGCGC GCTACATGCA GCAGCACCGC CAGGAGTCGC AGTCGCCAGA TTACCACCAT
    CGCTATGCGA GCAATGGCAA ACTGCCCCGG GCGGCGGCAC TGTCTACCAA TGGCAATGGA
    TTTACCACCG GCACCACGCA AACGGTCACA GTGGATGTGC ATCACCAGCT CGGCGGAGGA
    GGAGGAGGTG CTGCCGCCGC GGCCAAGCAG TTGATGGCCA ACGGCATCAG TGGCCATAGT
    CGCAGCAGCA GCATGAGCCA CAACATCCAT GCGGCAGCCT ACAGCGAACT GAGTGCCGCA
    CCACCGGCCT TCGCCACACC GCCGACGCGT AGGCGGTTCT TTAACCACAA GAATCTGCGC
    AGTGCCCTCA CTGGAGGAAG TGGCGGTGGA TCGGGAGTCG GCGGTGGTGT AGGAGGAGCT
    GGCGGTGTGG GCGGCGGTCA TCGACGCACT GCCTCCAATG GGGGTTGTCC CATTGACACA
    GCATCGATCC TGTCGGGAGA TCATACTCAT CAGCATCATC ACCGCGACAA TCAGCCGGAG
    GGCAAGGAGT CCAATGCCCT TAACACATCC ACCTGCTCGG ACTCGGCGGT CACTAGGAGG
    CGGAGGAAGA ACGTGAGCAA TCACAACCTG AAGACCTCTG CCCGCCATGG AGCAAGTAGC
    GAGAATCGTC TGAACAGGCT CAGTTTGGCC GGAACGTCCG TCTATGCGGG TCATCTGTCC
    TCTTTGGTCT TTGGCAAGAT CAAGTCCCTG TGGAGCGTGA ACTCCTCCAA TTCCAGCGAG
    GCGGGACTGA ATCAATTAGC AGGAAGCGAC GCTATTGACC ACCACTCCTC CTTCCTGAAT
    GAGAAACTGC AGAAGGATCA GCTCCACGCC CGTCTCGGTC TGCTGCTCAA CGATCCCGGC
    AGCAATGGAA ACAGCAGCAG CAGCGGAAGT GGTTGTGAAC CCATCTCGGC CCATTCCACC
    ACCAGCACCA CGAGCAGCAG CGGCGTGGGA GCGGCCAGCA CCACCACGAG CGGTTCCTCG
    CAGAATGTCA GTCCGGAGCA AACGCTGGCC AGCGGAGCCG GAATGCTCAG TGGCAGTCAG
    CTGTCGGTGG CCACCAGTCA TGGGGTCAAG GAGGATGCAC TGAGTCTGTG CGGCAAGTTC
    AAGGCTGGCT GCTCCATGCT GCACGTCTAC GAAGCATTGC CCAGCAAGAG TCGCAAAGGA
    AATGTACGGA GATCGACGCG TGGTCAGCAG GGATCCAGTT CGAGCGCATC GGCGGCATCA
    GCAGTGGGAT CCAGGGTGAC AGCCTCCAGT TTGGCGGCTG TGCAGTTGGC CCTGAAGCCA
    CTGTTCTTTG AGGTGCCGCT GCAGGAACCG GATCCACCAT ATGTGGGTCG CCAGTGGCTG
    GTGCAGCAGT TGTCCAACAT CCTGCTTGGC ACCGAGACAC GGGTGGTGCT CATTAACGGA
    CAGCCGGGCA CTGGAAAGAC GGCGTTTTGT CTGCAGCTGG TGGAGTACTC CTGCTTTGGC
    CGCCGGCAGA TGCAGGATGA TCCCGATGGC ATATACAGTC AGCTACAGTT GGGCGCCCAC
    TGTGAGCGCA TGCGCGGACT GGCCTCCCAC ATGGTTGGCT ATCACTTCTG CCAGGCGGAC
    GCCAATCTCA CCTGCCAGGT GCCCGATTTC GTGCATTCTC TGGCCGCCCA ACTGTGTCAG
    GCGCCGCAGT TAACCGCCTA CAGGGATTAC CTGCTCTCGG AGCCGCATCT TCAGGACATT
    CTCAGTGTGC GAGAGTGCAT CGCGGATGCG GAGAGAGTAA TGAAGCTGGC TATACTGGAA
    CCACTGGCTC ACCTTCACCG GGCGGGAAAG ATACCCGCCA AAGTGGCCGT CATCGTGGTG
    GATGCCTTGT GCGAAGCGGA GTACCATCGT CCCGATCATG GTCACACCAT TGCTAGTTTC
    TTGGCACAGC TTACGCCACA TTTTCCAGCT TGGCTCAAGC TGGTGGCCAC TGTGAGGACC
    CAGATGTTGG AGCTGGTGAA GGCACCCAGC TACACACAAC TTACCCTGGA CAGTTGGGCC
    AGCAGCCAGG CGTTGCAGCA GGATATGCTT GATTACATAG GCGCTCGGCT TGCAGATAGT
    CCGGAAATTC GAATGAACAT CGGCGGCGGA GGAGGCCAGA ACTCGCAGTC GGGCAGCCAG
    CCGCAGACGA AGTTCGTGTC CCATCTTCAG TCCCTGAGCA GGGGCTCCAT GCTGTACGCT
    AAGCTCATCC TGGATCTCAT TGCACGCGGC CAGTTGGTTA TCAAGTCATC CAGCTACAAG
    GTGCTGCCGG TCTCTCTTGC TCAGATCTTT CTGCTGCACT TCAACCTGCG CTTTCCCACC
    GCCCGTAGCT TTGAGCAGGC TGCTCCGATC CTCAATATAT GCCTGGCTGC TCTGTATCCT
    CTGACGCTGG ACGAGATCTA CTACAGTATG GAGGCACTGA GTCACGGACG GGAGGCACTA
    AGCTGGCCGG ACTTCATGCA ACGCTTTAAG CTGCTGGATG GATTTTTAAT CAAGAGGTTG
    GACAACACGT ACATGTTCTT TCATAGCTCC TTGAGGGAGT GGCTCATGCG AAGGGATGAG
    GGGGAGTCCA ACAAGTTCCT CTGCGACGCC CGTTTGGGTC ATGCTGGAAT CGCTTTCCGA
    TTATCCCGAC TGCAGGCTCC ATTGTCACCT CAGCTCACAT TGGAACTGGG TCACCACATG
    CTGAAAGCAC ATTTATACGG AGGAACTAGT CTGACGCTGC TTTCTCCGCG GGATTTGCAA
    TCTTATTGGT TGGCGGGAGC GGCGGATAAC ATTTCATCCT CGCTGGGCGC CCTGCGAAAT
    GTCTACAGTC CGAACCTAAA GGTTTCCCGA CTAGTTCTCT TGGCAGGAGC TTCACCGAAT
    CACCGCACGG ATTACATGGG TGGAGCTCCG ATCCTTTGCA TAGCTGCGCA TGAGGGAATC
    CTTCCCATGG TTAGCCTGCT CCTAGAATTC GGTGCCGATG TCGGTCTGAC CAACAGCCAG
    GGCTGTACTC CATTAATACT GGCCGCCATG CGTGGCCACT GCGACGTAGT GCGTCCATTG
    GTAGCCGCCG GCAGCAGCCT CGGTCAGTTG GACATCACGC AGCGCTGTGC CCTAGTTCAT
    GCCGCCCGCA TGGGTCACCT CAGTGTGGTC AAGTACTTGC TCGCCTGCGA CTGGTCACCA
    CGACCGCACT CCCAAGATGT CACCAGATCG GTTGCCTTGC AACAGGCGCT CATTGGAGCT
    GCCGCACAGG CGCACTGCAA GATCCTTGAG GATCTTCTGG ATCTCAATGA GACCGAGTTT
    GACCTGGACG TGAATGGCAT GGAGCCAAGC AGTGGTGAAC TGGCGCTAAC CGCCGCCGCA
    CGGCACGGAT GCATTGATGT TGTAGGAATC TTGCTCTCCC GTGGCGCTCA GATCGATGCA
    AGAAATCGGC AGGGATACTC GGCGCTTTGG TTGGCTGTCA AGGAGGGTCA CTGGAGTGTG
    GTGGAGCATC TGCTGCAACG TGGTGCCCTG TTGGATGAAC CATTGGGACA GACACGCAAG
    ACGCCGCTAA TGATTGCGGC GGAGGAGGGA CACCTGGAGC TGGTCGATCT GCTGCTGGCG
    CGGGGAGCGC AACGAGAAGC TCAGGATCAC GAGGGATTCA CTGCACTCAG CTGGGCCTGT
    TTACGTGGCC ACTTGGCGGC GGCCAAGACG CTCATAGAAC ATGGCTGTAA TCGCCACCAC
    GAGGATCACA ATGGACGCAC AGCCCTGGAT CTGGCAGCCT ATCAGGGAGC CGCTAGCCTG
    GTGATCTATA TCCTGGAGCA GGGCGGCAAT CTGGAACACA TTGATGTTCA TGGAATGCGT
    CCCCTGGATC GGGCCATTGC CTGCCGTAAC ATCCAGGCCG TACAGGTGTT CCTGCGCAAG
    GGGGCCAAAC TGGGACCGAC CACTTGGAGC ATGGCCATGG GCAAGCCGGA GATTCTGGTC
    ATACTACTGA ACAAGCTGCT CGAGGACGGT AATGTGCTGT ACCGGAAAAA CCGCTTCCAG
    GAGGCTGCTC ATCGCTATCA GTATGCCCTA CGCAAGATCT CCGGACTGGA GCAGTTGCTC
    GAAAGGAATG CCATCTTCGC CCAGCTGCGG ACCAATCTTC TGCTGAATCT GTCGCGCTGC
    AAGCGAAAAC TGAATGAACT TGACGCCTCC ATAGATTTGG CCACGCAAGC GATTGCCCAG
    AAGCCGCACA GCTACGAAGG ATACTACGCC AGGGCAAAGG CGCGCATGGA ACTTGGTGCA
    TTGAATGAAG CACTTGTGGA CGCCAATGAG GCGATGCAGC AGGCCGCCCA AAGCGGTGTG
    CTCTGCGAAG TGGTCGAGGT GCTGAAGCGC ATCCAGACCG AGCTGCTCAC GCGGATCAGC
    ATCAGCAGCG AGGATCAGAC CGGCAGGATG TCGAATATCG GCGGCGGGGC ATCCGTCGCA
    TACGAATCTC ATCATGAGAT CACCGATTTG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.