Plod cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Plod
Entrez Gene ID	39265
Full Name	procollagen lysyl hydroxylase
Synonyms	CG6199,Dmel\CG6199,LH3,RE-H,anon-EST:ParkEST325,anon-WO0118547.416,dPlod
General protein information	Preferred Names procollagen lysyl hydroxylase Names CG6199 gene product from transcript CG6199-RB CG6199-PA CG6199-PB Plod-PA Plod-PB drosophila plod lysyl hydroxylase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 68B1-68B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_140194.2	NP_648451.1	procollagen lysyl hydroxylase, isoform A
NM_168452.2	NP_729687.1	procollagen lysyl hydroxylase, isoform B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PLOD3	XP_536856.2
Mus musculus (house mouse)	Plod3	NP_036092.1
Pan troglodytes (chimpanzee)	PLOD3	XP_001153684.1
Bos taurus (cattle)	PLOD3	NP_001180184.1
Rattus norvegicus (Norway rat)	Plod3	NP_835202.2
Danio rerio (zebrafish)	wu:fb72h05	XP_002666609.1
Caenorhabditis elegans (roundworm)	let-268	NP_496170.1
Danio rerio (zebrafish)	plod3	NP_001037808.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001507	XP_321614.4
Homo sapiens (human)	PLOD3	NP_001075.1
	LOC714283	XP_002808395.1
Drosophila melanogaster (fruit fly)	Plod	NP_729687.1
Xenopus tropicalis (tropical clawed frog)	plod3	NP_001039229.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study characterised in detail the developmental expression patterns of dPlod RNA and protein during embryogenesis and find that dPlod is highly expressed in type-IV collagen-producing cells, particularly the haemocytes and fat body
Title: Characterisation of the Drosophila procollagen lysyl hydroxylase, dPlod.

The following Plod gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Plod cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18231	NM_140194.2 Latest version!	Drosophila melanogaster procollagen lysyl hydroxylase (Plod), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa18231	NM_168452.2 Latest version!	Drosophila melanogaster procollagen lysyl hydroxylase (Plod), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Plod ( NM_140194.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648451.1 Drosophila melanogaster procollagen lysyl hydroxylase (Plod), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18231
Clone ID Related Accession (Same CDS sequence)	NM_140194.2 , NM_168452.2
Accession Version	NM_140194.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	procollagen lysyl hydroxylase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140194.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGAATAC AACAAAGCGC CTTGTTGTTG TTGCTGCTGG CCGTGACGTC GCAAGGAGAT 
GCCGAGTCCA ATTGGAATGA CAAAATCAAA GTGTTCACTG TGGCCACTGA ACCCACCGAT 
GGCTATACTA GATATATTCG ATCTGCACGA GTCTACGATA TTGAGGTAAC CACCCTTGGC 
TTGGGAGAGG AATGGAAAGG AGGAGATATG CAAAAGCCTG GCGGCGGATT TAAGCTCAAC 
CTTCTGCGCG AAGCTATTGC TCCGTATAAA AATGAACCCG AAACAATCAT ACTCTTCACA 
GACAGCTACG ATGTGATTAT CACCACCACG CTGGATGAGA TCTTTGAGAA ATTCAAGGAG 
TCTGGTGCCA AGATACTCTT CTCCGCCGAG AAATACTGCT GGCCCGACAA AAGTCTGGCC 
AATGACTATC CCGAAGTGGA GGGCAAGGCC TCCAGATTCC TCAACTCGGG CGCGTTCATT 
GGTTATGCAC CACAAGTTTT CGCTCTGCTG GTGGATCCAA TTGAAGACAC TGCCGACGAT 
CAGCTATATT TTACAAAGAT CTTCCTTGAC GAGACCAAAC GCGCCAAGCT GGGCTTAAAG 
CTGGACGTAC AATCGCGCCT ATTCCAAAAC CTACATGGTG CCAAGAACGA TGTGAAGCTT 
AAAGTGGATC TCGAGAGCAA TCAGGGCGTT CTACAGAACG TGGATTTCAT GACAACGCCT 
TCCATCATCC ATGGCAATGG ACTTAGCAAA GTGGATCTGA ATGCATATGG CAACTATTTG 
GCCAGGACTT TTAATGGCGT CTGCCTTTTG TGTCAGGAAA ATCTTTTAGA CTTGGAAGAG 
ACAAACCTAC CTGTGATTTC CCTAGCCCTG ATGGTCACGC AACCCGTACC CTTCTTCGAC 
CAGTTTCTGG AGGGCATCGA GAGTCTGAAC TATCCTAAGG AAAAACTTCA CTTGCTCATC 
TACAGCAATG TTGCATTTCA CGACGATGAC ATCAAGTCGT TTGTAAACAA ACATGCCAAA 
GAGTATGCTA CTGCCAAATT TGCTCTTTCC ACTGACGAGT TGGATGAACG CCAGGGCAGG 
CAGTTGGCCC TTGACAAGGC TCGTCTCCAC CAGAGTGATT ATATATTCTT TGTGGATGCA 
GATGCGCACA TCGACGATGG CGAGGTTCTC CGGGAGCTGC TCAGGCTGAA CAAGCAATTC 
GTGGCTCCGA TTTTCTCCAA GCACAAGGAG CTGTGGAGCA ACTTCTGGGG CGCTCTTTCC 
GAGGGAGGCT ACTATGCCAG GTCGCACGAT TACGTGGACA TTGTAAAGCG GGAACTTATT 
GGAATGTTCA ATGTGCCCCA TGTAACGAGC ATATATCTGG TGAAGAAAAC CGCCTTCGAT 
GCCATCTCCT TCAAGCACAA GGAATTCGAT CCCGACATGG CCATGTGCGA GTCACTCCGA 
AATGCGGGTA TCTTCATGTA CGCCAGCAAT CTGCGCATAT TCGGTCACTT GGTCAACGCC 
GATGACTTCA ACACTACGGT GACACGTCCG GACTTTTATA CGTTGTTTAG CAACGAGATC 
GACTGGACGG AGAAGTACAT ACATCCGAAC TACTCACTGC AGCTGAACGA GAGCAACAAG 
ATACAGCAGC CGTGCCCGGA TGTCTATTGG TTCCAGATCG TGTCGGACGC GTTCTGCGAC 
GATTTGGTGG CCATCATGGA GGCGCACAAC GGTTGGTCGG ACGGCAGCAA CAACGACAAT 
CGGCTGGAGG GCGGATATGA GGCGGTGCCC ACCAGGGATA TCCACATGAA GCAGGTGGGC 
CTGGAGCGGC TCTATCTTAA ATTTCTTCAA ATGTTCGTTC GTCCGCTGCA GGAGCGCGCC 
TTTACGGGTT ACTTCCACAA TCCTCCACGC GCGCTGATGA ACTTCATGGT GCGTTATCGG 
CCGGATGAAC AGCCTTCTCT GCGACCCCAC CACGACTCCT CCACGTACAC CATCAACATT 
GCGATGAACC GGGCGGGTAT TGACTATCAG GGCGGTGGCT GTCGTTTCAT TCGTTACAAC 
TGCTCCGTGA CCGATACGAA GAAGGGATGG ATGCTGATGC ACCCGGGTCG ACTGACTCAC 
TACCACGAGG GATTGCTAGT GACCAATGGC ACGCGTTACA TCATGATTTC CTTCATCGAT 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648451.1
CDS	205..2370
Translation	MRIQQSALLLLLLAVTSQGDAESNWNDKIKVFTVATEPTDGYTRYIRSARVYDIEVTTLGLGEEWKGGDMQKPGGGFKLNLLREAIAPYKNEPETIILFTDSYDVIITTTLDEIFEKFKESGAKILFSAEKYCWPDKSLANDYPEVEGKASRFLNSGAFIGYAPQVFALLVDPIEDTADDQLYFTKIFLDETKRAKLGLKLDVQSRLFQNLHGAKNDVKLKVDLESNQGVLQNVDFMTTPSIIHGNGLSKVDLNAYGNYLARTFNGVCLLCQENLLDLEETNLPVISLALMVTQPVPFFDQFLEGIESLNYPKEKLHLLIYSNVAFHDDDIKSFVNKHAKEYATAKFALSTDELDERQGRQLALDKARLHQSDYIFFVDADAHIDDGEVLRELLRLNKQFVAPIFSKHKELWSNFWGALSEGGYYARSHDYVDIVKRELIGMFNVPHVTSIYLVKKTAFDAISFKHKEFDPDMAMCESLRNAGIFMYASNLRIFGHLVNADDFNTTVTRPDFYTLFSNEIDWTEKYIHPNYSLQLNESNKIQQPCPDVYWFQIVSDAFCDDLVAIMEAHNGWSDGSNNDNRLEGGYEAVPTRDIHMKQVGLERLYLKFLQMFVRPLQERAFTGYFHNPPRALMNFMVRYRPDEQPSLRPHHDSSTYTINIAMNRAGIDYQGGGCRFIRYNCSVTDTKKGWMLMHPGRLTHYHEGLLVTNGTRYIMISFIDP

Target ORF information:

RefSeq Version	NM_140194.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster procollagen lysyl hydroxylase (Plod), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140194.2

ORF Insert Sequence:

ATGAGAATAC AACAAAGCGC CTTGTTGTTG TTGCTGCTGG CCGTGACGTC GCAAGGAGAT 
GCCGAGTCCA ATTGGAATGA CAAAATCAAA GTGTTCACTG TGGCCACTGA ACCCACCGAT 
GGCTATACTA GATATATTCG ATCTGCACGA GTCTACGATA TTGAGGTAAC CACCCTTGGC 
TTGGGAGAGG AATGGAAAGG AGGAGATATG CAAAAGCCTG GCGGCGGATT TAAGCTCAAC 
CTTCTGCGCG AAGCTATTGC TCCGTATAAA AATGAACCCG AAACAATCAT ACTCTTCACA 
GACAGCTACG ATGTGATTAT CACCACCACG CTGGATGAGA TCTTTGAGAA ATTCAAGGAG 
TCTGGTGCCA AGATACTCTT CTCCGCCGAG AAATACTGCT GGCCCGACAA AAGTCTGGCC 
AATGACTATC CCGAAGTGGA GGGCAAGGCC TCCAGATTCC TCAACTCGGG CGCGTTCATT 
GGTTATGCAC CACAAGTTTT CGCTCTGCTG GTGGATCCAA TTGAAGACAC TGCCGACGAT 
CAGCTATATT TTACAAAGAT CTTCCTTGAC GAGACCAAAC GCGCCAAGCT GGGCTTAAAG 
CTGGACGTAC AATCGCGCCT ATTCCAAAAC CTACATGGTG CCAAGAACGA TGTGAAGCTT 
AAAGTGGATC TCGAGAGCAA TCAGGGCGTT CTACAGAACG TGGATTTCAT GACAACGCCT 
TCCATCATCC ATGGCAATGG ACTTAGCAAA GTGGATCTGA ATGCATATGG CAACTATTTG 
GCCAGGACTT TTAATGGCGT CTGCCTTTTG TGTCAGGAAA ATCTTTTAGA CTTGGAAGAG 
ACAAACCTAC CTGTGATTTC CCTAGCCCTG ATGGTCACGC AACCCGTACC CTTCTTCGAC 
CAGTTTCTGG AGGGCATCGA GAGTCTGAAC TATCCTAAGG AAAAACTTCA CTTGCTCATC 
TACAGCAATG TTGCATTTCA CGACGATGAC ATCAAGTCGT TTGTAAACAA ACATGCCAAA 
GAGTATGCTA CTGCCAAATT TGCTCTTTCC ACTGACGAGT TGGATGAACG CCAGGGCAGG 
CAGTTGGCCC TTGACAAGGC TCGTCTCCAC CAGAGTGATT ATATATTCTT TGTGGATGCA 
GATGCGCACA TCGACGATGG CGAGGTTCTC CGGGAGCTGC TCAGGCTGAA CAAGCAATTC 
GTGGCTCCGA TTTTCTCCAA GCACAAGGAG CTGTGGAGCA ACTTCTGGGG CGCTCTTTCC 
GAGGGAGGCT ACTATGCCAG GTCGCACGAT TACGTGGACA TTGTAAAGCG GGAACTTATT 
GGAATGTTCA ATGTGCCCCA TGTAACGAGC ATATATCTGG TGAAGAAAAC CGCCTTCGAT 
GCCATCTCCT TCAAGCACAA GGAATTCGAT CCCGACATGG CCATGTGCGA GTCACTCCGA 
AATGCGGGTA TCTTCATGTA CGCCAGCAAT CTGCGCATAT TCGGTCACTT GGTCAACGCC 
GATGACTTCA ACACTACGGT GACACGTCCG GACTTTTATA CGTTGTTTAG CAACGAGATC 
GACTGGACGG AGAAGTACAT ACATCCGAAC TACTCACTGC AGCTGAACGA GAGCAACAAG 
ATACAGCAGC CGTGCCCGGA TGTCTATTGG TTCCAGATCG TGTCGGACGC GTTCTGCGAC 
GATTTGGTGG CCATCATGGA GGCGCACAAC GGTTGGTCGG ACGGCAGCAA CAACGACAAT 
CGGCTGGAGG GCGGATATGA GGCGGTGCCC ACCAGGGATA TCCACATGAA GCAGGTGGGC 
CTGGAGCGGC TCTATCTTAA ATTTCTTCAA ATGTTCGTTC GTCCGCTGCA GGAGCGCGCC 
TTTACGGGTT ACTTCCACAA TCCTCCACGC GCGCTGATGA ACTTCATGGT GCGTTATCGG 
CCGGATGAAC AGCCTTCTCT GCGACCCCAC CACGACTCCT CCACGTACAC CATCAACATT 
GCGATGAACC GGGCGGGTAT TGACTATCAG GGCGGTGGCT GTCGTTTCAT TCGTTACAAC 
TGCTCCGTGA CCGATACGAA GAAGGGATGG ATGCTGATGC ACCCGGGTCG ACTGACTCAC 
TACCACGAGG GATTGCTAGT GACCAATGGC ACGCGTTACA TCATGATTTC CTTCATCGAT 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plod ( NM_168452.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729687.1 Drosophila melanogaster procollagen lysyl hydroxylase (Plod), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18231
Clone ID Related Accession (Same CDS sequence)	NM_140194.2 , NM_168452.2
Accession Version	NM_168452.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	procollagen lysyl hydroxylase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168452.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGAATAC AACAAAGCGC CTTGTTGTTG TTGCTGCTGG CCGTGACGTC GCAAGGAGAT 
GCCGAGTCCA ATTGGAATGA CAAAATCAAA GTGTTCACTG TGGCCACTGA ACCCACCGAT 
GGCTATACTA GATATATTCG ATCTGCACGA GTCTACGATA TTGAGGTAAC CACCCTTGGC 
TTGGGAGAGG AATGGAAAGG AGGAGATATG CAAAAGCCTG GCGGCGGATT TAAGCTCAAC 
CTTCTGCGCG AAGCTATTGC TCCGTATAAA AATGAACCCG AAACAATCAT ACTCTTCACA 
GACAGCTACG ATGTGATTAT CACCACCACG CTGGATGAGA TCTTTGAGAA ATTCAAGGAG 
TCTGGTGCCA AGATACTCTT CTCCGCCGAG AAATACTGCT GGCCCGACAA AAGTCTGGCC 
AATGACTATC CCGAAGTGGA GGGCAAGGCC TCCAGATTCC TCAACTCGGG CGCGTTCATT 
GGTTATGCAC CACAAGTTTT CGCTCTGCTG GTGGATCCAA TTGAAGACAC TGCCGACGAT 
CAGCTATATT TTACAAAGAT CTTCCTTGAC GAGACCAAAC GCGCCAAGCT GGGCTTAAAG 
CTGGACGTAC AATCGCGCCT ATTCCAAAAC CTACATGGTG CCAAGAACGA TGTGAAGCTT 
AAAGTGGATC TCGAGAGCAA TCAGGGCGTT CTACAGAACG TGGATTTCAT GACAACGCCT 
TCCATCATCC ATGGCAATGG ACTTAGCAAA GTGGATCTGA ATGCATATGG CAACTATTTG 
GCCAGGACTT TTAATGGCGT CTGCCTTTTG TGTCAGGAAA ATCTTTTAGA CTTGGAAGAG 
ACAAACCTAC CTGTGATTTC CCTAGCCCTG ATGGTCACGC AACCCGTACC CTTCTTCGAC 
CAGTTTCTGG AGGGCATCGA GAGTCTGAAC TATCCTAAGG AAAAACTTCA CTTGCTCATC 
TACAGCAATG TTGCATTTCA CGACGATGAC ATCAAGTCGT TTGTAAACAA ACATGCCAAA 
GAGTATGCTA CTGCCAAATT TGCTCTTTCC ACTGACGAGT TGGATGAACG CCAGGGCAGG 
CAGTTGGCCC TTGACAAGGC TCGTCTCCAC CAGAGTGATT ATATATTCTT TGTGGATGCA 
GATGCGCACA TCGACGATGG CGAGGTTCTC CGGGAGCTGC TCAGGCTGAA CAAGCAATTC 
GTGGCTCCGA TTTTCTCCAA GCACAAGGAG CTGTGGAGCA ACTTCTGGGG CGCTCTTTCC 
GAGGGAGGCT ACTATGCCAG GTCGCACGAT TACGTGGACA TTGTAAAGCG GGAACTTATT 
GGAATGTTCA ATGTGCCCCA TGTAACGAGC ATATATCTGG TGAAGAAAAC CGCCTTCGAT 
GCCATCTCCT TCAAGCACAA GGAATTCGAT CCCGACATGG CCATGTGCGA GTCACTCCGA 
AATGCGGGTA TCTTCATGTA CGCCAGCAAT CTGCGCATAT TCGGTCACTT GGTCAACGCC 
GATGACTTCA ACACTACGGT GACACGTCCG GACTTTTATA CGTTGTTTAG CAACGAGATC 
GACTGGACGG AGAAGTACAT ACATCCGAAC TACTCACTGC AGCTGAACGA GAGCAACAAG 
ATACAGCAGC CGTGCCCGGA TGTCTATTGG TTCCAGATCG TGTCGGACGC GTTCTGCGAC 
GATTTGGTGG CCATCATGGA GGCGCACAAC GGTTGGTCGG ACGGCAGCAA CAACGACAAT 
CGGCTGGAGG GCGGATATGA GGCGGTGCCC ACCAGGGATA TCCACATGAA GCAGGTGGGC 
CTGGAGCGGC TCTATCTTAA ATTTCTTCAA ATGTTCGTTC GTCCGCTGCA GGAGCGCGCC 
TTTACGGGTT ACTTCCACAA TCCTCCACGC GCGCTGATGA ACTTCATGGT GCGTTATCGG 
CCGGATGAAC AGCCTTCTCT GCGACCCCAC CACGACTCCT CCACGTACAC CATCAACATT 
GCGATGAACC GGGCGGGTAT TGACTATCAG GGCGGTGGCT GTCGTTTCAT TCGTTACAAC 
TGCTCCGTGA CCGATACGAA GAAGGGATGG ATGCTGATGC ACCCGGGTCG ACTGACTCAC 
TACCACGAGG GATTGCTAGT GACCAATGGC ACGCGTTACA TCATGATTTC CTTCATCGAT 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729687.1
CDS	119..2284
Translation	MRIQQSALLLLLLAVTSQGDAESNWNDKIKVFTVATEPTDGYTRYIRSARVYDIEVTTLGLGEEWKGGDMQKPGGGFKLNLLREAIAPYKNEPETIILFTDSYDVIITTTLDEIFEKFKESGAKILFSAEKYCWPDKSLANDYPEVEGKASRFLNSGAFIGYAPQVFALLVDPIEDTADDQLYFTKIFLDETKRAKLGLKLDVQSRLFQNLHGAKNDVKLKVDLESNQGVLQNVDFMTTPSIIHGNGLSKVDLNAYGNYLARTFNGVCLLCQENLLDLEETNLPVISLALMVTQPVPFFDQFLEGIESLNYPKEKLHLLIYSNVAFHDDDIKSFVNKHAKEYATAKFALSTDELDERQGRQLALDKARLHQSDYIFFVDADAHIDDGEVLRELLRLNKQFVAPIFSKHKELWSNFWGALSEGGYYARSHDYVDIVKRELIGMFNVPHVTSIYLVKKTAFDAISFKHKEFDPDMAMCESLRNAGIFMYASNLRIFGHLVNADDFNTTVTRPDFYTLFSNEIDWTEKYIHPNYSLQLNESNKIQQPCPDVYWFQIVSDAFCDDLVAIMEAHNGWSDGSNNDNRLEGGYEAVPTRDIHMKQVGLERLYLKFLQMFVRPLQERAFTGYFHNPPRALMNFMVRYRPDEQPSLRPHHDSSTYTINIAMNRAGIDYQGGGCRFIRYNCSVTDTKKGWMLMHPGRLTHYHEGLLVTNGTRYIMISFIDP

Target ORF information:

RefSeq Version	NM_168452.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster procollagen lysyl hydroxylase (Plod), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168452.2

ORF Insert Sequence:

ATGAGAATAC AACAAAGCGC CTTGTTGTTG TTGCTGCTGG CCGTGACGTC GCAAGGAGAT 
GCCGAGTCCA ATTGGAATGA CAAAATCAAA GTGTTCACTG TGGCCACTGA ACCCACCGAT 
GGCTATACTA GATATATTCG ATCTGCACGA GTCTACGATA TTGAGGTAAC CACCCTTGGC 
TTGGGAGAGG AATGGAAAGG AGGAGATATG CAAAAGCCTG GCGGCGGATT TAAGCTCAAC 
CTTCTGCGCG AAGCTATTGC TCCGTATAAA AATGAACCCG AAACAATCAT ACTCTTCACA 
GACAGCTACG ATGTGATTAT CACCACCACG CTGGATGAGA TCTTTGAGAA ATTCAAGGAG 
TCTGGTGCCA AGATACTCTT CTCCGCCGAG AAATACTGCT GGCCCGACAA AAGTCTGGCC 
AATGACTATC CCGAAGTGGA GGGCAAGGCC TCCAGATTCC TCAACTCGGG CGCGTTCATT 
GGTTATGCAC CACAAGTTTT CGCTCTGCTG GTGGATCCAA TTGAAGACAC TGCCGACGAT 
CAGCTATATT TTACAAAGAT CTTCCTTGAC GAGACCAAAC GCGCCAAGCT GGGCTTAAAG 
CTGGACGTAC AATCGCGCCT ATTCCAAAAC CTACATGGTG CCAAGAACGA TGTGAAGCTT 
AAAGTGGATC TCGAGAGCAA TCAGGGCGTT CTACAGAACG TGGATTTCAT GACAACGCCT 
TCCATCATCC ATGGCAATGG ACTTAGCAAA GTGGATCTGA ATGCATATGG CAACTATTTG 
GCCAGGACTT TTAATGGCGT CTGCCTTTTG TGTCAGGAAA ATCTTTTAGA CTTGGAAGAG 
ACAAACCTAC CTGTGATTTC CCTAGCCCTG ATGGTCACGC AACCCGTACC CTTCTTCGAC 
CAGTTTCTGG AGGGCATCGA GAGTCTGAAC TATCCTAAGG AAAAACTTCA CTTGCTCATC 
TACAGCAATG TTGCATTTCA CGACGATGAC ATCAAGTCGT TTGTAAACAA ACATGCCAAA 
GAGTATGCTA CTGCCAAATT TGCTCTTTCC ACTGACGAGT TGGATGAACG CCAGGGCAGG 
CAGTTGGCCC TTGACAAGGC TCGTCTCCAC CAGAGTGATT ATATATTCTT TGTGGATGCA 
GATGCGCACA TCGACGATGG CGAGGTTCTC CGGGAGCTGC TCAGGCTGAA CAAGCAATTC 
GTGGCTCCGA TTTTCTCCAA GCACAAGGAG CTGTGGAGCA ACTTCTGGGG CGCTCTTTCC 
GAGGGAGGCT ACTATGCCAG GTCGCACGAT TACGTGGACA TTGTAAAGCG GGAACTTATT 
GGAATGTTCA ATGTGCCCCA TGTAACGAGC ATATATCTGG TGAAGAAAAC CGCCTTCGAT 
GCCATCTCCT TCAAGCACAA GGAATTCGAT CCCGACATGG CCATGTGCGA GTCACTCCGA 
AATGCGGGTA TCTTCATGTA CGCCAGCAAT CTGCGCATAT TCGGTCACTT GGTCAACGCC 
GATGACTTCA ACACTACGGT GACACGTCCG GACTTTTATA CGTTGTTTAG CAACGAGATC 
GACTGGACGG AGAAGTACAT ACATCCGAAC TACTCACTGC AGCTGAACGA GAGCAACAAG 
ATACAGCAGC CGTGCCCGGA TGTCTATTGG TTCCAGATCG TGTCGGACGC GTTCTGCGAC 
GATTTGGTGG CCATCATGGA GGCGCACAAC GGTTGGTCGG ACGGCAGCAA CAACGACAAT 
CGGCTGGAGG GCGGATATGA GGCGGTGCCC ACCAGGGATA TCCACATGAA GCAGGTGGGC 
CTGGAGCGGC TCTATCTTAA ATTTCTTCAA ATGTTCGTTC GTCCGCTGCA GGAGCGCGCC 
TTTACGGGTT ACTTCCACAA TCCTCCACGC GCGCTGATGA ACTTCATGGT GCGTTATCGG 
CCGGATGAAC AGCCTTCTCT GCGACCCCAC CACGACTCCT CCACGTACAC CATCAACATT 
GCGATGAACC GGGCGGGTAT TGACTATCAG GGCGGTGGCT GTCGTTTCAT TCGTTACAAC 
TGCTCCGTGA CCGATACGAA GAAGGGATGG ATGCTGATGC ACCCGGGTCG ACTGACTCAC 
TACCACGAGG GATTGCTAGT GACCAATGGC ACGCGTTACA TCATGATTTC CTTCATCGAT 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
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Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
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The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
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Plod cDNA ORF clone, Drosophila melanogaster(fruit fly)

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ORF Insert Sequence:

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