NaPi-III cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	NaPi-III
Entrez Gene ID	39255
Full Name	Na[+]-dependent inorganic phosphate cotransporter type III
Synonyms	CG42575,CG7628,CR32078,Dmel\CG42575,Dmel_CG7628,Dmel_CR32078,Dromel_CG7628_FBtr0076213_mORF,dPit
General protein information	Preferred Names Na[+]-dependent inorganic phosphate cotransporter type III Names CG42575 gene product from transcript CG42575-RA CG42575-PA CG42575-PB CR32078 NaPi-III-PA NaPi-III-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 68A7-68A7

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274775.1	NP_001261704.1	Na[+]-dependent inorganic phosphate cotransporter type III, isoform B
NM_140184.4	NP_648441.1	Na[+]-dependent inorganic phosphate cotransporter type III, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG42575	NP_648441.1
Danio rerio (zebrafish)	slc20a1a	NP_998344.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004251	XP_003436758.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NaPi-III gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NaPi-III cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18217	NM_001274775.1 Latest version!	Drosophila melanogaster Na[+]-dependent inorganic phosphate cotransporter type III (NaPi-III), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa18217	NM_140184.4 Latest version!	Drosophila melanogaster Na[+]-dependent inorganic phosphate cotransporter type III (NaPi-III), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NaPi-III ( NM_001274775.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261704.1 Drosophila melanogaster Na[+]-dependent inorganic phosphate cotransporter type III (NaPi-III), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18217
Clone ID Related Accession (Same CDS sequence)	NM_001274775.1 , NM_140184.4
Accession Version	NM_001274775.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Na[+]-dependent inorganic phosphate cotransporter type III, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAGCT TTGCGCCGGA ATTACTTTGG ATGGTAGTCA TCGGATTCCT AATCGCCTTC 
GTACTCGCCT TTGGCATCGG TGCCAACGAC GTGGCCAACT CATTTGGCAC CAGTGTCGGC 
TCCGGTGTTC TGACCATCCG GCAGGCATGC GTGCTGGCCA CCATTTGCGA AATTTCGGGT 
GCCGTTTTGA TTGGCTACAA GGTATCGGAT ACCATGCGCA AGGGAATCCT AGAAGTTGGC 
CTATACGAGG GCGCCGAGGA GGTTCTGATG CTGGGCTGCG TGGCCGCATT GGCCAGTAGC 
GCCGTTTGGC TGCTGGTGGC CACCTTTTTG AAGTTGCCTA TTTCGGGAAC GCACAGTATC 
GTTGGCTCTA CCATTGGATT TTCGCTAGTG GCGCGCGGTG TACAGGGCCT AAAGTGGTCT 
ACTTTGGGCA CAATCGTCGG ATCGTGGTTC ATCTCACCGG TGCTGAGTGG AATTGTGAGC 
ATTCTGCTCT TCCTGGCCAT TCGTCGTTTC ATCCTACGTG CCCAGGAGCC GCTCAAGGCC 
GGATTTCGAT CGCTGCCCAT CTTCTACGGT GTAACGTTCT TCATCAACGT TATCAGCGTT 
GTGTTGGACG GGCCCAAGCT GCTCTACATG GACAACATAC CCACTTGGAT AGCACTGACC 
GCCAGTTTTG GTCTCTCTTT GCTGGTGGCA TTGCTTACGC AATTGGTAGT GGTGCCTTTG 
CAGCGGCGCA AGATTGCCAA ACGATTGCGG GCCGAGAATC CTGTCAAGTT TAATTTCGAG 
GACTCCGTGG AATCTTCCCC GTCGGGAAGT CCCAAGAAGC AGCGTCGTCC CCTGTCGCTG 
GTTAGCGAAG GAAAGCCACT GCCTGCCATC GCAGAAATAA CCGAGCTGGT CTCGTTGAGC 
GACAACTCGC CGAGGACCTT TAAGTTGGCT CCATTCGGAT TGGCGGCCAA GAACAACAAT 
GCGCTTGGGG AGGAGTACAA GATCGATCCG CAGCTGATCA AAAAGGCTGA GGATCTGCTG 
GGCAAGGCTA GCCTGGACAA CACGGATCTG ACGATTACCA GTCTGAATTT TATCGACGAG 
CAGCAGCAAC AACAGCAGCA GCAGCAAAAT GGACGCAAGT TGCAGGAGTG CTTCAAGCGG 
ATGCAGTCGC CCAAGGAGGA GCAGAAGAGC AAGACGAACT CCATTGGCAC GGACTTGGAA 
ACCGGATCGA CCAAAGCCAC AAATAACAAC CTTCAAGTGG TGGAGAGCGG CGGCAGCCTT 
GATCTTATGA TCAGTTCCAC TTTATCGCCC AATTCCAGCA AGGTTCCTCT GATCGAGAGC 
AAGGAGGCGC TGAATGAGCA GGAGGAGGAG CTGAAACGAA CGACGGGCGG TGGACGAAGA 
ACCAGTGGCG CCGAGGAGAC TCCGGAGATT TCAATGCTCT TCTCGTTTCT GCAGATCCTG 
ACAGCCACAT TTGGCAGTTT TGCGCACGGC GGCAATGATG TGAGCAACGC CATCGGCCCA 
CTGATTGCCC TCTATATGAT CTATCGCGAG GGATCGGTTA TGCAGCAGGC CGAGAGTCCC 
ATCTATATTC TGATCTACGG CGGCATCGGC ATCTCTGTGG GTCTCTGGCT GTGGGGACGA 
CGAGTCATCG AGACCATTGG CAATGACCTT ACGAAAATAA CCTCATCGAC TGGCTTTACT 
ATTGAAGTCG GAGCTGCCAT CACCGTACTG CTGGCCAGCA AAATTGGCCT GCCCATTTCG 
ACTACTCACT GCAAGGTCGG TTCGGTGGTT TTTGTGGGTC ATGTGAGTGC ATCAGGTCGC 
AAGAAGAAGA GTCAGCCGAA TGATCAGACC GATAAGGAGA CCCATAACGA AGTTGCGCCC 
ATGGATGATG GCAGTGTCGA TTGGCATCTA TTCCGGAATA TTGCCTATGC ATGGATCGTA 
ACGGTGCCGG TTACGGCGCT CCTCAGCGCC GGAATGATGT ACGTGCTGTG TGCCATCGCC 
GTGGACGACA TGGGTGGGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261704.1
CDS	287..2290
Translation	MESFAPELLWMVVIGFLIAFVLAFGIGANDVANSFGTSVGSGVLTIRQACVLATICEISGAVLIGYKVSDTMRKGILEVGLYEGAEEVLMLGCVAALASSAVWLLVATFLKLPISGTHSIVGSTIGFSLVARGVQGLKWSTLGTIVGSWFISPVLSGIVSILLFLAIRRFILRAQEPLKAGFRSLPIFYGVTFFINVISVVLDGPKLLYMDNIPTWIALTASFGLSLLVALLTQLVVVPLQRRKIAKRLRAENPVKFNFEDSVESSPSGSPKKQRRPLSLVSEGKPLPAIAEITELVSLSDNSPRTFKLAPFGLAAKNNNALGEEYKIDPQLIKKAEDLLGKASLDNTDLTITSLNFIDEQQQQQQQQQNGRKLQECFKRMQSPKEEQKSKTNSIGTDLETGSTKATNNNLQVVESGGSLDLMISSTLSPNSSKVPLIESKEALNEQEEELKRTTGGGRRTSGAEETPEISMLFSFLQILTATFGSFAHGGNDVSNAIGPLIALYMIYREGSVMQQAESPIYILIYGGIGISVGLWLWGRRVIETIGNDLTKITSSTGFTIEVGAAITVLLASKIGLPISTTHCKVGSVVFVGHVSASGRKKKSQPNDQTDKETHNEVAPMDDGSVDWHLFRNIAYAWIVTVPVTALLSAGMMYVLCAIAVDDMGGA

Target ORF information:

RefSeq Version	NM_001274775.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Na[+]-dependent inorganic phosphate cotransporter type III (NaPi-III), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274775.1

ORF Insert Sequence:

ATGGAGAGCT TTGCGCCGGA ATTACTTTGG ATGGTAGTCA TCGGATTCCT AATCGCCTTC 
GTACTCGCCT TTGGCATCGG TGCCAACGAC GTGGCCAACT CATTTGGCAC CAGTGTCGGC 
TCCGGTGTTC TGACCATCCG GCAGGCATGC GTGCTGGCCA CCATTTGCGA AATTTCGGGT 
GCCGTTTTGA TTGGCTACAA GGTATCGGAT ACCATGCGCA AGGGAATCCT AGAAGTTGGC 
CTATACGAGG GCGCCGAGGA GGTTCTGATG CTGGGCTGCG TGGCCGCATT GGCCAGTAGC 
GCCGTTTGGC TGCTGGTGGC CACCTTTTTG AAGTTGCCTA TTTCGGGAAC GCACAGTATC 
GTTGGCTCTA CCATTGGATT TTCGCTAGTG GCGCGCGGTG TACAGGGCCT AAAGTGGTCT 
ACTTTGGGCA CAATCGTCGG ATCGTGGTTC ATCTCACCGG TGCTGAGTGG AATTGTGAGC 
ATTCTGCTCT TCCTGGCCAT TCGTCGTTTC ATCCTACGTG CCCAGGAGCC GCTCAAGGCC 
GGATTTCGAT CGCTGCCCAT CTTCTACGGT GTAACGTTCT TCATCAACGT TATCAGCGTT 
GTGTTGGACG GGCCCAAGCT GCTCTACATG GACAACATAC CCACTTGGAT AGCACTGACC 
GCCAGTTTTG GTCTCTCTTT GCTGGTGGCA TTGCTTACGC AATTGGTAGT GGTGCCTTTG 
CAGCGGCGCA AGATTGCCAA ACGATTGCGG GCCGAGAATC CTGTCAAGTT TAATTTCGAG 
GACTCCGTGG AATCTTCCCC GTCGGGAAGT CCCAAGAAGC AGCGTCGTCC CCTGTCGCTG 
GTTAGCGAAG GAAAGCCACT GCCTGCCATC GCAGAAATAA CCGAGCTGGT CTCGTTGAGC 
GACAACTCGC CGAGGACCTT TAAGTTGGCT CCATTCGGAT TGGCGGCCAA GAACAACAAT 
GCGCTTGGGG AGGAGTACAA GATCGATCCG CAGCTGATCA AAAAGGCTGA GGATCTGCTG 
GGCAAGGCTA GCCTGGACAA CACGGATCTG ACGATTACCA GTCTGAATTT TATCGACGAG 
CAGCAGCAAC AACAGCAGCA GCAGCAAAAT GGACGCAAGT TGCAGGAGTG CTTCAAGCGG 
ATGCAGTCGC CCAAGGAGGA GCAGAAGAGC AAGACGAACT CCATTGGCAC GGACTTGGAA 
ACCGGATCGA CCAAAGCCAC AAATAACAAC CTTCAAGTGG TGGAGAGCGG CGGCAGCCTT 
GATCTTATGA TCAGTTCCAC TTTATCGCCC AATTCCAGCA AGGTTCCTCT GATCGAGAGC 
AAGGAGGCGC TGAATGAGCA GGAGGAGGAG CTGAAACGAA CGACGGGCGG TGGACGAAGA 
ACCAGTGGCG CCGAGGAGAC TCCGGAGATT TCAATGCTCT TCTCGTTTCT GCAGATCCTG 
ACAGCCACAT TTGGCAGTTT TGCGCACGGC GGCAATGATG TGAGCAACGC CATCGGCCCA 
CTGATTGCCC TCTATATGAT CTATCGCGAG GGATCGGTTA TGCAGCAGGC CGAGAGTCCC 
ATCTATATTC TGATCTACGG CGGCATCGGC ATCTCTGTGG GTCTCTGGCT GTGGGGACGA 
CGAGTCATCG AGACCATTGG CAATGACCTT ACGAAAATAA CCTCATCGAC TGGCTTTACT 
ATTGAAGTCG GAGCTGCCAT CACCGTACTG CTGGCCAGCA AAATTGGCCT GCCCATTTCG 
ACTACTCACT GCAAGGTCGG TTCGGTGGTT TTTGTGGGTC ATGTGAGTGC ATCAGGTCGC 
AAGAAGAAGA GTCAGCCGAA TGATCAGACC GATAAGGAGA CCCATAACGA AGTTGCGCCC 
ATGGATGATG GCAGTGTCGA TTGGCATCTA TTCCGGAATA TTGCCTATGC ATGGATCGTA 
ACGGTGCCGG TTACGGCGCT CCTCAGCGCC GGAATGATGT ACGTGCTGTG TGCCATCGCC 
GTGGACGACA TGGGTGGGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NaPi-III ( NM_140184.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648441.1 Drosophila melanogaster Na[+]-dependent inorganic phosphate cotransporter type III (NaPi-III), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18217
Clone ID Related Accession (Same CDS sequence)	NM_001274775.1 , NM_140184.4
Accession Version	NM_140184.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Na[+]-dependent inorganic phosphate cotransporter type III, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140184.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAGCT TTGCGCCGGA ATTACTTTGG ATGGTAGTCA TCGGATTCCT AATCGCCTTC 
GTACTCGCCT TTGGCATCGG TGCCAACGAC GTGGCCAACT CATTTGGCAC CAGTGTCGGC 
TCCGGTGTTC TGACCATCCG GCAGGCATGC GTGCTGGCCA CCATTTGCGA AATTTCGGGT 
GCCGTTTTGA TTGGCTACAA GGTATCGGAT ACCATGCGCA AGGGAATCCT AGAAGTTGGC 
CTATACGAGG GCGCCGAGGA GGTTCTGATG CTGGGCTGCG TGGCCGCATT GGCCAGTAGC 
GCCGTTTGGC TGCTGGTGGC CACCTTTTTG AAGTTGCCTA TTTCGGGAAC GCACAGTATC 
GTTGGCTCTA CCATTGGATT TTCGCTAGTG GCGCGCGGTG TACAGGGCCT AAAGTGGTCT 
ACTTTGGGCA CAATCGTCGG ATCGTGGTTC ATCTCACCGG TGCTGAGTGG AATTGTGAGC 
ATTCTGCTCT TCCTGGCCAT TCGTCGTTTC ATCCTACGTG CCCAGGAGCC GCTCAAGGCC 
GGATTTCGAT CGCTGCCCAT CTTCTACGGT GTAACGTTCT TCATCAACGT TATCAGCGTT 
GTGTTGGACG GGCCCAAGCT GCTCTACATG GACAACATAC CCACTTGGAT AGCACTGACC 
GCCAGTTTTG GTCTCTCTTT GCTGGTGGCA TTGCTTACGC AATTGGTAGT GGTGCCTTTG 
CAGCGGCGCA AGATTGCCAA ACGATTGCGG GCCGAGAATC CTGTCAAGTT TAATTTCGAG 
GACTCCGTGG AATCTTCCCC GTCGGGAAGT CCCAAGAAGC AGCGTCGTCC CCTGTCGCTG 
GTTAGCGAAG GAAAGCCACT GCCTGCCATC GCAGAAATAA CCGAGCTGGT CTCGTTGAGC 
GACAACTCGC CGAGGACCTT TAAGTTGGCT CCATTCGGAT TGGCGGCCAA GAACAACAAT 
GCGCTTGGGG AGGAGTACAA GATCGATCCG CAGCTGATCA AAAAGGCTGA GGATCTGCTG 
GGCAAGGCTA GCCTGGACAA CACGGATCTG ACGATTACCA GTCTGAATTT TATCGACGAG 
CAGCAGCAAC AACAGCAGCA GCAGCAAAAT GGACGCAAGT TGCAGGAGTG CTTCAAGCGG 
ATGCAGTCGC CCAAGGAGGA GCAGAAGAGC AAGACGAACT CCATTGGCAC GGACTTGGAA 
ACCGGATCGA CCAAAGCCAC AAATAACAAC CTTCAAGTGG TGGAGAGCGG CGGCAGCCTT 
GATCTTATGA TCAGTTCCAC TTTATCGCCC AATTCCAGCA AGGTTCCTCT GATCGAGAGC 
AAGGAGGCGC TGAATGAGCA GGAGGAGGAG CTGAAACGAA CGACGGGCGG TGGACGAAGA 
ACCAGTGGCG CCGAGGAGAC TCCGGAGATT TCAATGCTCT TCTCGTTTCT GCAGATCCTG 
ACAGCCACAT TTGGCAGTTT TGCGCACGGC GGCAATGATG TGAGCAACGC CATCGGCCCA 
CTGATTGCCC TCTATATGAT CTATCGCGAG GGATCGGTTA TGCAGCAGGC CGAGAGTCCC 
ATCTATATTC TGATCTACGG CGGCATCGGC ATCTCTGTGG GTCTCTGGCT GTGGGGACGA 
CGAGTCATCG AGACCATTGG CAATGACCTT ACGAAAATAA CCTCATCGAC TGGCTTTACT 
ATTGAAGTCG GAGCTGCCAT CACCGTACTG CTGGCCAGCA AAATTGGCCT GCCCATTTCG 
ACTACTCACT GCAAGGTCGG TTCGGTGGTT TTTGTGGGTC ATGTGAGTGC ATCAGGTCGC 
AAGAAGAAGA GTCAGCCGAA TGATCAGACC GATAAGGAGA CCCATAACGA AGTTGCGCCC 
ATGGATGATG GCAGTGTCGA TTGGCATCTA TTCCGGAATA TTGCCTATGC ATGGATCGTA 
ACGGTGCCGG TTACGGCGCT CCTCAGCGCC GGAATGATGT ACGTGCTGTG TGCCATCGCC 
GTGGACGACA TGGGTGGGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648441.1
CDS	1049..3052
Translation	MESFAPELLWMVVIGFLIAFVLAFGIGANDVANSFGTSVGSGVLTIRQACVLATICEISGAVLIGYKVSDTMRKGILEVGLYEGAEEVLMLGCVAALASSAVWLLVATFLKLPISGTHSIVGSTIGFSLVARGVQGLKWSTLGTIVGSWFISPVLSGIVSILLFLAIRRFILRAQEPLKAGFRSLPIFYGVTFFINVISVVLDGPKLLYMDNIPTWIALTASFGLSLLVALLTQLVVVPLQRRKIAKRLRAENPVKFNFEDSVESSPSGSPKKQRRPLSLVSEGKPLPAIAEITELVSLSDNSPRTFKLAPFGLAAKNNNALGEEYKIDPQLIKKAEDLLGKASLDNTDLTITSLNFIDEQQQQQQQQQNGRKLQECFKRMQSPKEEQKSKTNSIGTDLETGSTKATNNNLQVVESGGSLDLMISSTLSPNSSKVPLIESKEALNEQEEELKRTTGGGRRTSGAEETPEISMLFSFLQILTATFGSFAHGGNDVSNAIGPLIALYMIYREGSVMQQAESPIYILIYGGIGISVGLWLWGRRVIETIGNDLTKITSSTGFTIEVGAAITVLLASKIGLPISTTHCKVGSVVFVGHVSASGRKKKSQPNDQTDKETHNEVAPMDDGSVDWHLFRNIAYAWIVTVPVTALLSAGMMYVLCAIAVDDMGGA

Target ORF information:

RefSeq Version	NM_140184.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Na[+]-dependent inorganic phosphate cotransporter type III (NaPi-III), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140184.4

ORF Insert Sequence:

ATGGAGAGCT TTGCGCCGGA ATTACTTTGG ATGGTAGTCA TCGGATTCCT AATCGCCTTC 
GTACTCGCCT TTGGCATCGG TGCCAACGAC GTGGCCAACT CATTTGGCAC CAGTGTCGGC 
TCCGGTGTTC TGACCATCCG GCAGGCATGC GTGCTGGCCA CCATTTGCGA AATTTCGGGT 
GCCGTTTTGA TTGGCTACAA GGTATCGGAT ACCATGCGCA AGGGAATCCT AGAAGTTGGC 
CTATACGAGG GCGCCGAGGA GGTTCTGATG CTGGGCTGCG TGGCCGCATT GGCCAGTAGC 
GCCGTTTGGC TGCTGGTGGC CACCTTTTTG AAGTTGCCTA TTTCGGGAAC GCACAGTATC 
GTTGGCTCTA CCATTGGATT TTCGCTAGTG GCGCGCGGTG TACAGGGCCT AAAGTGGTCT 
ACTTTGGGCA CAATCGTCGG ATCGTGGTTC ATCTCACCGG TGCTGAGTGG AATTGTGAGC 
ATTCTGCTCT TCCTGGCCAT TCGTCGTTTC ATCCTACGTG CCCAGGAGCC GCTCAAGGCC 
GGATTTCGAT CGCTGCCCAT CTTCTACGGT GTAACGTTCT TCATCAACGT TATCAGCGTT 
GTGTTGGACG GGCCCAAGCT GCTCTACATG GACAACATAC CCACTTGGAT AGCACTGACC 
GCCAGTTTTG GTCTCTCTTT GCTGGTGGCA TTGCTTACGC AATTGGTAGT GGTGCCTTTG 
CAGCGGCGCA AGATTGCCAA ACGATTGCGG GCCGAGAATC CTGTCAAGTT TAATTTCGAG 
GACTCCGTGG AATCTTCCCC GTCGGGAAGT CCCAAGAAGC AGCGTCGTCC CCTGTCGCTG 
GTTAGCGAAG GAAAGCCACT GCCTGCCATC GCAGAAATAA CCGAGCTGGT CTCGTTGAGC 
GACAACTCGC CGAGGACCTT TAAGTTGGCT CCATTCGGAT TGGCGGCCAA GAACAACAAT 
GCGCTTGGGG AGGAGTACAA GATCGATCCG CAGCTGATCA AAAAGGCTGA GGATCTGCTG 
GGCAAGGCTA GCCTGGACAA CACGGATCTG ACGATTACCA GTCTGAATTT TATCGACGAG 
CAGCAGCAAC AACAGCAGCA GCAGCAAAAT GGACGCAAGT TGCAGGAGTG CTTCAAGCGG 
ATGCAGTCGC CCAAGGAGGA GCAGAAGAGC AAGACGAACT CCATTGGCAC GGACTTGGAA 
ACCGGATCGA CCAAAGCCAC AAATAACAAC CTTCAAGTGG TGGAGAGCGG CGGCAGCCTT 
GATCTTATGA TCAGTTCCAC TTTATCGCCC AATTCCAGCA AGGTTCCTCT GATCGAGAGC 
AAGGAGGCGC TGAATGAGCA GGAGGAGGAG CTGAAACGAA CGACGGGCGG TGGACGAAGA 
ACCAGTGGCG CCGAGGAGAC TCCGGAGATT TCAATGCTCT TCTCGTTTCT GCAGATCCTG 
ACAGCCACAT TTGGCAGTTT TGCGCACGGC GGCAATGATG TGAGCAACGC CATCGGCCCA 
CTGATTGCCC TCTATATGAT CTATCGCGAG GGATCGGTTA TGCAGCAGGC CGAGAGTCCC 
ATCTATATTC TGATCTACGG CGGCATCGGC ATCTCTGTGG GTCTCTGGCT GTGGGGACGA 
CGAGTCATCG AGACCATTGG CAATGACCTT ACGAAAATAA CCTCATCGAC TGGCTTTACT 
ATTGAAGTCG GAGCTGCCAT CACCGTACTG CTGGCCAGCA AAATTGGCCT GCCCATTTCG 
ACTACTCACT GCAAGGTCGG TTCGGTGGTT TTTGTGGGTC ATGTGAGTGC ATCAGGTCGC 
AAGAAGAAGA GTCAGCCGAA TGATCAGACC GATAAGGAGA CCCATAACGA AGTTGCGCCC 
ATGGATGATG GCAGTGTCGA TTGGCATCTA TTCCGGAATA TTGCCTATGC ATGGATCGTA 
ACGGTGCCGG TTACGGCGCT CCTCAGCGCC GGAATGATGT ACGTGCTGTG TGCCATCGCC 
GTGGACGACA TGGGTGGGGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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NaPi-III cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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