klu cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	klu
Entrez Gene ID	39228
Full Name	klumpfuss
Synonyms	CG12296,Dmel\CG12296,KLU,Klu,P09036,l(3)09036,l(3)10052
General protein information	Preferred Names klumpfuss Names CG12296 gene product from transcript CG12296-RD CG12296-PA CG12296-PB CG12296-PC CG12296-PD klu-PA klu-PB klu-PC klu-PD klumphuss
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 68A1-68A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274761.1	NP_001261690.1	klumpfuss, isoform B
NM_001300095.1	NP_001287024.1	klumpfuss, isoform D
NM_057714.4	NP_477062.1	klumpfuss, isoform A
NM_001274762.1	NP_001261691.1	klumpfuss, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Klu does not lie downstream of the Notch pathway in asymmetric cell division context.
Title: A new role of Klumpfuss in establishing cell fate during the GMC asymmetric cell division.

the Brat-dependent mechanism extinguishes the function of the self-renewal factor Klu in the presumptive intermediate progenitor cell by attenuating Arm activity, balancing stem cell maintenance and progenitor cell specification.
Title: Brain tumor specifies intermediate progenitor cell identity by attenuating ?-catenin/Armadillo activity.

Klu is expressed specifically in the postmitotic Ap4/FMRFa neuron, promoting its differentiation through the initiation of BMP signaling
Title: Klumpfuss controls FMRFamide expression by enabling BMP signaling within the NB5-6 lineage.

FACS purification and transcriptome analysis of drosophila neural stem cells reveals a role for Klumpfuss in self-renewal.
Title: FACS purification and transcriptome analysis of drosophila neural stem cells reveals a role for Klumpfuss in self-renewal.

multiple mechanisms converge to exert precise control of klu and distinguish a progenitor cell from its sibling stem cell during asymmetric neuroblast division
Title: klumpfuss distinguishes stem cells from progenitor cells during asymmetric neuroblast division.

The following klu gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the klu cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18190	NM_057714.4 Latest version!	Drosophila melanogaster klumpfuss (klu), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa18190	NM_001300095.1 Latest version!	Drosophila melanogaster klumpfuss (klu), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa18190	NM_001274762.1 Latest version!	Drosophila melanogaster klumpfuss (klu), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa19896	NM_001274761.1 Latest version!	Drosophila melanogaster klumpfuss (klu), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

klu ( NM_001300095.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287024.1 Drosophila melanogaster klumpfuss (klu), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa18190
Clone ID Related Accession (Same CDS sequence)	NM_001300095.1 , NM_057714.4 , NM_001274762.1
Accession Version	NM_001300095.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	klumpfuss, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGATGG CAGAAGGCAC CGACCCCATG GGGCATGGTC ACCACACGAA TCCGCACAAT 
CCGCCCCAGC AGCCGCCGCA GCACCATCCG CACCACCTGC CCCTGCCCCA TCCCCCGCCG 
GCACCACCGC CGCAGCTACT CAATTTCAAT CACCACCAGC AGCAGTATCT TCCCCACATG 
TATCCTGCGG CAGCGGCAGC GGCACAATCT CACTCCCACT ACGGTGGCCT GCAGTATCCC 
CATCCACATG CGCTGCAGCA TCAGCATCAT CCCCACCAGC ACCAGCATCA CTTGCCGCTG 
GCCTTGAGCC TCCATCAGCA GGCGGCTGCG GCGGCGGCAG TGGCTGCAGC GGTGGCCACC 
AACCACTCCA ACAATAACAA TCAGGCCAGC GTGGCCAACA ATAATATAGT GGTTCCATGG 
AAGCAGCGCA AGCGCAAGAG GCTCTCAGCG GTGCTGGACA AGCTGCACCA CAATAATAAT 
AATAATAATA ACAACAATAA TGAGACCCAC TCCAATGGCA TGGCCTGCAA GGCGGAACCG 
ATGGAAATGA AGGGGGATCT AGCCGAAGAT GCCGAACAGG ATGAAGATCA TGATGGGTCC 
GTGCCTGATG AGGAAGATGA TTCCGTAGAT CAGGATGGTG ATGCTGGCAA GTACATCAAG 
AGCGAGCAGC CCGTCAGCGA GGACGAGGAG AACGAACAGG AAATGGACCA AGATATCGAG 
GACATTTCCG GCGAGGATCT CGAGCTGTCG CACAGCGATG ATAATGACCA GGACGACAGC 
CCCAGGATCA GTATGAGTCC TCTGCAGCTG CAGGCCGCCC AGAGCAATCT GCCCCAAGAT 
CAGAACCAGC CGATGAACAA GGAGAATCCG CTTCATGTGG ACATCAAGAC TGAGATCCCC 
AGCCCCTATG ATAGGTATTT CCCTATACCA TCTCCTCTGT TCGGCTATTA CCTGCACACC 
AAATATCTGA ATGAGGTATT CCGCCGGCGT CATGATCTCT ATCCCTCGCC TCTGCAGCAC 
ACTCCATCTT CCATAGCCTC CGAAACGGAG ACTTCACCCA ATGCTCAGGA GCGCAAGAGT 
AGCTCCAACC ACGTCTTGCC CCACGCTCTG TTGGCCAATA ATTCACCTCC GCCATCGCTG 
CCATCGCCAC CGCGCAGCGA ATCCTCGGTG ACTAACAATG TGGCCACCAC CACCACTAGC 
AGCACCACCA AGAAGCGCAG CAGTCCCAAG CCCAAGGGCA AGAAGGGTGA GAAGAAGCCA 
ATGCCACCGC CGCAAGAGCG TCCCTTGGAT CTGTGCATGC GCAACGAAGT GGAGCCCAAG 
AAGTACAAAA AGTCAGGCTC GAAATCCTCT CTAGAGTCCC GTTCCGCCGG TATGATGCCG 
CCGCCGCCGG CTCTGAGTGC GGCCAGCAGT TTGGAGAGCA TGAGTGCCCT ATCACCGGCA 
TCGAGTAGCC ACTCGGGTCA CATGCCCATC ACCAGCGCTG CCACGCCCAA CCACCAGCCA 
CCACCCAATT CCCCCTATGC CAATGCCATG AACGCTGCCC ATGCAGCGGC AGCAGCGGCG 
GCGGCAGCCA TGATCAAGAT GGAGATGCCT TTGCATCCGC TGCACCACCA GCAGATGCAT 
CACTCGCAAG TGCCCACCAC CACAGTGGGT GTGCCGGTGA TCAAGGGCGA TGTGGCCTCG 
CCCACAACAA AGGAAACGGT CGCCTGGCGT TACAATCTGG ACGTGTCGCC CGTGGTTGAG 
GAGATGCCGC CCGGTTCGGA TGTGGCTTAT GTATGCCCCA CCTGCGGACA GATGTTCTCG 
CTGCACGACC GCCTGGCCAA GCACATGGCC TCGCGTCACA AGTCCCGCAA TCCCGCCAAT 
GATATTGCCA AAGCCTATTC CTGCGATGTG TGCCGGCGAT CCTTTGCCCG CTCCGATATG 
CTGACCCGCC ACATGCGCCT CCACACCGGA GTCAAGCCGT ACACGTGCAA GGTGTGCGGT 
CAGGTCTTTT CCCGCTCGGA TCACCTGTCC ACCCACCAGA GGACCCACAC CGGCGAGAAG 
CCCTACAAGT GTCCGCAGTG CCCGTATGCC GCCTGTCGTC GCGACATGAT CACCAGGCAT 
ATGCGGACAC ACACGCGGTA CGATTCCAGG GGAGGATCTC GAGAAGGCAG GGAAGGACGC 
GAAGGACGTG AGGGCAAGGA GAGCCGCGAT TCGCGCAGGA GCAACGAACG AATCGAGGAG 
CCCAACTCGC CCGGTTCCCA TTCGCTGCTG GACATGAAGA TGAACATGAA CATGGGCATG 
GGAATGGGCC TCAATATGGG CCTACCCATG AATCCGATGA GTCTACTGCA GGAGGAACTG 
CTGCAGAAGT CCCAGCCGGG CCTAACCCTG GGTGGACCCA TGCCACTGGT TGTCAAGACG 
GAGAGCGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287024.1
CDS	1062..3473
Translation	MTMAEGTDPMGHGHHTNPHNPPQQPPQHHPHHLPLPHPPPAPPPQLLNFNHHQQQYLPHMYPAAAAAAQSHSHYGGLQYPHPHALQHQHHPHQHQHHLPLALSLHQQAAAAAAVAAAVATNHSNNNNQASVANNNIVVPWKQRKRKRLSAVLDKLHHNNNNNNNNNNETHSNGMACKAEPMEMKGDLAEDAEQDEDHDGSVPDEEDDSVDQDGDAGKYIKSEQPVSEDEENEQEMDQDIEDISGEDLELSHSDDNDQDDSPRISMSPLQLQAAQSNLPQDQNQPMNKENPLHVDIKTEIPSPYDRYFPIPSPLFGYYLHTKYLNEVFRRRHDLYPSPLQHTPSSIASETETSPNAQERKSSSNHVLPHALLANNSPPPSLPSPPRSESSVTNNVATTTTSSTTKKRSSPKPKGKKGEKKPMPPPQERPLDLCMRNEVEPKKYKKSGSKSSLESRSAGMMPPPPALSAASSLESMSALSPASSSHSGHMPITSAATPNHQPPPNSPYANAMNAAHAAAAAAAAAMIKMEMPLHPLHHQQMHHSQVPTTTVGVPVIKGDVASPTTKETVAWRYNLDVSPVVEEMPPGSDVAYVCPTCGQMFSLHDRLAKHMASRHKSRNPANDIAKAYSCDVCRRSFARSDMLTRHMRLHTGVKPYTCKVCGQVFSRSDHLSTHQRTHTGEKPYKCPQCPYAACRRDMITRHMRTHTRYDSRGGSREGREGREGREGKESRDSRRSNERIEEPNSPGSHSLLDMKMNMNMGMGMGLNMGLPMNPMSLLQEELLQKSQPGLTLGGPMPLVVKTESA

Target ORF information:

RefSeq Version	NM_001300095.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster klumpfuss (klu), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300095.1

ORF Insert Sequence:

ATGACGATGG CAGAAGGCAC CGACCCCATG GGGCATGGTC ACCACACGAA TCCGCACAAT 
CCGCCCCAGC AGCCGCCGCA GCACCATCCG CACCACCTGC CCCTGCCCCA TCCCCCGCCG 
GCACCACCGC CGCAGCTACT CAATTTCAAT CACCACCAGC AGCAGTATCT TCCCCACATG 
TATCCTGCGG CAGCGGCAGC GGCACAATCT CACTCCCACT ACGGTGGCCT GCAGTATCCC 
CATCCACATG CGCTGCAGCA TCAGCATCAT CCCCACCAGC ACCAGCATCA CTTGCCGCTG 
GCCTTGAGCC TCCATCAGCA GGCGGCTGCG GCGGCGGCAG TGGCTGCAGC GGTGGCCACC 
AACCACTCCA ACAATAACAA TCAGGCCAGC GTGGCCAACA ATAATATAGT GGTTCCATGG 
AAGCAGCGCA AGCGCAAGAG GCTCTCAGCG GTGCTGGACA AGCTGCACCA CAATAATAAT 
AATAATAATA ACAACAATAA TGAGACCCAC TCCAATGGCA TGGCCTGCAA GGCGGAACCG 
ATGGAAATGA AGGGGGATCT AGCCGAAGAT GCCGAACAGG ATGAAGATCA TGATGGGTCC 
GTGCCTGATG AGGAAGATGA TTCCGTAGAT CAGGATGGTG ATGCTGGCAA GTACATCAAG 
AGCGAGCAGC CCGTCAGCGA GGACGAGGAG AACGAACAGG AAATGGACCA AGATATCGAG 
GACATTTCCG GCGAGGATCT CGAGCTGTCG CACAGCGATG ATAATGACCA GGACGACAGC 
CCCAGGATCA GTATGAGTCC TCTGCAGCTG CAGGCCGCCC AGAGCAATCT GCCCCAAGAT 
CAGAACCAGC CGATGAACAA GGAGAATCCG CTTCATGTGG ACATCAAGAC TGAGATCCCC 
AGCCCCTATG ATAGGTATTT CCCTATACCA TCTCCTCTGT TCGGCTATTA CCTGCACACC 
AAATATCTGA ATGAGGTATT CCGCCGGCGT CATGATCTCT ATCCCTCGCC TCTGCAGCAC 
ACTCCATCTT CCATAGCCTC CGAAACGGAG ACTTCACCCA ATGCTCAGGA GCGCAAGAGT 
AGCTCCAACC ACGTCTTGCC CCACGCTCTG TTGGCCAATA ATTCACCTCC GCCATCGCTG 
CCATCGCCAC CGCGCAGCGA ATCCTCGGTG ACTAACAATG TGGCCACCAC CACCACTAGC 
AGCACCACCA AGAAGCGCAG CAGTCCCAAG CCCAAGGGCA AGAAGGGTGA GAAGAAGCCA 
ATGCCACCGC CGCAAGAGCG TCCCTTGGAT CTGTGCATGC GCAACGAAGT GGAGCCCAAG 
AAGTACAAAA AGTCAGGCTC GAAATCCTCT CTAGAGTCCC GTTCCGCCGG TATGATGCCG 
CCGCCGCCGG CTCTGAGTGC GGCCAGCAGT TTGGAGAGCA TGAGTGCCCT ATCACCGGCA 
TCGAGTAGCC ACTCGGGTCA CATGCCCATC ACCAGCGCTG CCACGCCCAA CCACCAGCCA 
CCACCCAATT CCCCCTATGC CAATGCCATG AACGCTGCCC ATGCAGCGGC AGCAGCGGCG 
GCGGCAGCCA TGATCAAGAT GGAGATGCCT TTGCATCCGC TGCACCACCA GCAGATGCAT 
CACTCGCAAG TGCCCACCAC CACAGTGGGT GTGCCGGTGA TCAAGGGCGA TGTGGCCTCG 
CCCACAACAA AGGAAACGGT CGCCTGGCGT TACAATCTGG ACGTGTCGCC CGTGGTTGAG 
GAGATGCCGC CCGGTTCGGA TGTGGCTTAT GTATGCCCCA CCTGCGGACA GATGTTCTCG 
CTGCACGACC GCCTGGCCAA GCACATGGCC TCGCGTCACA AGTCCCGCAA TCCCGCCAAT 
GATATTGCCA AAGCCTATTC CTGCGATGTG TGCCGGCGAT CCTTTGCCCG CTCCGATATG 
CTGACCCGCC ACATGCGCCT CCACACCGGA GTCAAGCCGT ACACGTGCAA GGTGTGCGGT 
CAGGTCTTTT CCCGCTCGGA TCACCTGTCC ACCCACCAGA GGACCCACAC CGGCGAGAAG 
CCCTACAAGT GTCCGCAGTG CCCGTATGCC GCCTGTCGTC GCGACATGAT CACCAGGCAT 
ATGCGGACAC ACACGCGGTA CGATTCCAGG GGAGGATCTC GAGAAGGCAG GGAAGGACGC 
GAAGGACGTG AGGGCAAGGA GAGCCGCGAT TCGCGCAGGA GCAACGAACG AATCGAGGAG 
CCCAACTCGC CCGGTTCCCA TTCGCTGCTG GACATGAAGA TGAACATGAA CATGGGCATG 
GGAATGGGCC TCAATATGGG CCTACCCATG AATCCGATGA GTCTACTGCA GGAGGAACTG 
CTGCAGAAGT CCCAGCCGGG CCTAACCCTG GGTGGACCCA TGCCACTGGT TGTCAAGACG 
GAGAGCGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

klu ( NM_057714.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477062.1 Drosophila melanogaster klumpfuss (klu), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18190
Clone ID Related Accession (Same CDS sequence)	NM_001300095.1 , NM_057714.4 , NM_001274762.1
Accession Version	NM_057714.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	klumpfuss, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_057714.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGATGG CAGAAGGCAC CGACCCCATG GGGCATGGTC ACCACACGAA TCCGCACAAT 
CCGCCCCAGC AGCCGCCGCA GCACCATCCG CACCACCTGC CCCTGCCCCA TCCCCCGCCG 
GCACCACCGC CGCAGCTACT CAATTTCAAT CACCACCAGC AGCAGTATCT TCCCCACATG 
TATCCTGCGG CAGCGGCAGC GGCACAATCT CACTCCCACT ACGGTGGCCT GCAGTATCCC 
CATCCACATG CGCTGCAGCA TCAGCATCAT CCCCACCAGC ACCAGCATCA CTTGCCGCTG 
GCCTTGAGCC TCCATCAGCA GGCGGCTGCG GCGGCGGCAG TGGCTGCAGC GGTGGCCACC 
AACCACTCCA ACAATAACAA TCAGGCCAGC GTGGCCAACA ATAATATAGT GGTTCCATGG 
AAGCAGCGCA AGCGCAAGAG GCTCTCAGCG GTGCTGGACA AGCTGCACCA CAATAATAAT 
AATAATAATA ACAACAATAA TGAGACCCAC TCCAATGGCA TGGCCTGCAA GGCGGAACCG 
ATGGAAATGA AGGGGGATCT AGCCGAAGAT GCCGAACAGG ATGAAGATCA TGATGGGTCC 
GTGCCTGATG AGGAAGATGA TTCCGTAGAT CAGGATGGTG ATGCTGGCAA GTACATCAAG 
AGCGAGCAGC CCGTCAGCGA GGACGAGGAG AACGAACAGG AAATGGACCA AGATATCGAG 
GACATTTCCG GCGAGGATCT CGAGCTGTCG CACAGCGATG ATAATGACCA GGACGACAGC 
CCCAGGATCA GTATGAGTCC TCTGCAGCTG CAGGCCGCCC AGAGCAATCT GCCCCAAGAT 
CAGAACCAGC CGATGAACAA GGAGAATCCG CTTCATGTGG ACATCAAGAC TGAGATCCCC 
AGCCCCTATG ATAGGTATTT CCCTATACCA TCTCCTCTGT TCGGCTATTA CCTGCACACC 
AAATATCTGA ATGAGGTATT CCGCCGGCGT CATGATCTCT ATCCCTCGCC TCTGCAGCAC 
ACTCCATCTT CCATAGCCTC CGAAACGGAG ACTTCACCCA ATGCTCAGGA GCGCAAGAGT 
AGCTCCAACC ACGTCTTGCC CCACGCTCTG TTGGCCAATA ATTCACCTCC GCCATCGCTG 
CCATCGCCAC CGCGCAGCGA ATCCTCGGTG ACTAACAATG TGGCCACCAC CACCACTAGC 
AGCACCACCA AGAAGCGCAG CAGTCCCAAG CCCAAGGGCA AGAAGGGTGA GAAGAAGCCA 
ATGCCACCGC CGCAAGAGCG TCCCTTGGAT CTGTGCATGC GCAACGAAGT GGAGCCCAAG 
AAGTACAAAA AGTCAGGCTC GAAATCCTCT CTAGAGTCCC GTTCCGCCGG TATGATGCCG 
CCGCCGCCGG CTCTGAGTGC GGCCAGCAGT TTGGAGAGCA TGAGTGCCCT ATCACCGGCA 
TCGAGTAGCC ACTCGGGTCA CATGCCCATC ACCAGCGCTG CCACGCCCAA CCACCAGCCA 
CCACCCAATT CCCCCTATGC CAATGCCATG AACGCTGCCC ATGCAGCGGC AGCAGCGGCG 
GCGGCAGCCA TGATCAAGAT GGAGATGCCT TTGCATCCGC TGCACCACCA GCAGATGCAT 
CACTCGCAAG TGCCCACCAC CACAGTGGGT GTGCCGGTGA TCAAGGGCGA TGTGGCCTCG 
CCCACAACAA AGGAAACGGT CGCCTGGCGT TACAATCTGG ACGTGTCGCC CGTGGTTGAG 
GAGATGCCGC CCGGTTCGGA TGTGGCTTAT GTATGCCCCA CCTGCGGACA GATGTTCTCG 
CTGCACGACC GCCTGGCCAA GCACATGGCC TCGCGTCACA AGTCCCGCAA TCCCGCCAAT 
GATATTGCCA AAGCCTATTC CTGCGATGTG TGCCGGCGAT CCTTTGCCCG CTCCGATATG 
CTGACCCGCC ACATGCGCCT CCACACCGGA GTCAAGCCGT ACACGTGCAA GGTGTGCGGT 
CAGGTCTTTT CCCGCTCGGA TCACCTGTCC ACCCACCAGA GGACCCACAC CGGCGAGAAG 
CCCTACAAGT GTCCGCAGTG CCCGTATGCC GCCTGTCGTC GCGACATGAT CACCAGGCAT 
ATGCGGACAC ACACGCGGTA CGATTCCAGG GGAGGATCTC GAGAAGGCAG GGAAGGACGC 
GAAGGACGTG AGGGCAAGGA GAGCCGCGAT TCGCGCAGGA GCAACGAACG AATCGAGGAG 
CCCAACTCGC CCGGTTCCCA TTCGCTGCTG GACATGAAGA TGAACATGAA CATGGGCATG 
GGAATGGGCC TCAATATGGG CCTACCCATG AATCCGATGA GTCTACTGCA GGAGGAACTG 
CTGCAGAAGT CCCAGCCGGG CCTAACCCTG GGTGGACCCA TGCCACTGGT TGTCAAGACG 
GAGAGCGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477062.1
CDS	707..3118
Translation	MTMAEGTDPMGHGHHTNPHNPPQQPPQHHPHHLPLPHPPPAPPPQLLNFNHHQQQYLPHMYPAAAAAAQSHSHYGGLQYPHPHALQHQHHPHQHQHHLPLALSLHQQAAAAAAVAAAVATNHSNNNNQASVANNNIVVPWKQRKRKRLSAVLDKLHHNNNNNNNNNNETHSNGMACKAEPMEMKGDLAEDAEQDEDHDGSVPDEEDDSVDQDGDAGKYIKSEQPVSEDEENEQEMDQDIEDISGEDLELSHSDDNDQDDSPRISMSPLQLQAAQSNLPQDQNQPMNKENPLHVDIKTEIPSPYDRYFPIPSPLFGYYLHTKYLNEVFRRRHDLYPSPLQHTPSSIASETETSPNAQERKSSSNHVLPHALLANNSPPPSLPSPPRSESSVTNNVATTTTSSTTKKRSSPKPKGKKGEKKPMPPPQERPLDLCMRNEVEPKKYKKSGSKSSLESRSAGMMPPPPALSAASSLESMSALSPASSSHSGHMPITSAATPNHQPPPNSPYANAMNAAHAAAAAAAAAMIKMEMPLHPLHHQQMHHSQVPTTTVGVPVIKGDVASPTTKETVAWRYNLDVSPVVEEMPPGSDVAYVCPTCGQMFSLHDRLAKHMASRHKSRNPANDIAKAYSCDVCRRSFARSDMLTRHMRLHTGVKPYTCKVCGQVFSRSDHLSTHQRTHTGEKPYKCPQCPYAACRRDMITRHMRTHTRYDSRGGSREGREGREGREGKESRDSRRSNERIEEPNSPGSHSLLDMKMNMNMGMGMGLNMGLPMNPMSLLQEELLQKSQPGLTLGGPMPLVVKTESA

Target ORF information:

RefSeq Version	NM_057714.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster klumpfuss (klu), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057714.4

ORF Insert Sequence:

ATGACGATGG CAGAAGGCAC CGACCCCATG GGGCATGGTC ACCACACGAA TCCGCACAAT 
CCGCCCCAGC AGCCGCCGCA GCACCATCCG CACCACCTGC CCCTGCCCCA TCCCCCGCCG 
GCACCACCGC CGCAGCTACT CAATTTCAAT CACCACCAGC AGCAGTATCT TCCCCACATG 
TATCCTGCGG CAGCGGCAGC GGCACAATCT CACTCCCACT ACGGTGGCCT GCAGTATCCC 
CATCCACATG CGCTGCAGCA TCAGCATCAT CCCCACCAGC ACCAGCATCA CTTGCCGCTG 
GCCTTGAGCC TCCATCAGCA GGCGGCTGCG GCGGCGGCAG TGGCTGCAGC GGTGGCCACC 
AACCACTCCA ACAATAACAA TCAGGCCAGC GTGGCCAACA ATAATATAGT GGTTCCATGG 
AAGCAGCGCA AGCGCAAGAG GCTCTCAGCG GTGCTGGACA AGCTGCACCA CAATAATAAT 
AATAATAATA ACAACAATAA TGAGACCCAC TCCAATGGCA TGGCCTGCAA GGCGGAACCG 
ATGGAAATGA AGGGGGATCT AGCCGAAGAT GCCGAACAGG ATGAAGATCA TGATGGGTCC 
GTGCCTGATG AGGAAGATGA TTCCGTAGAT CAGGATGGTG ATGCTGGCAA GTACATCAAG 
AGCGAGCAGC CCGTCAGCGA GGACGAGGAG AACGAACAGG AAATGGACCA AGATATCGAG 
GACATTTCCG GCGAGGATCT CGAGCTGTCG CACAGCGATG ATAATGACCA GGACGACAGC 
CCCAGGATCA GTATGAGTCC TCTGCAGCTG CAGGCCGCCC AGAGCAATCT GCCCCAAGAT 
CAGAACCAGC CGATGAACAA GGAGAATCCG CTTCATGTGG ACATCAAGAC TGAGATCCCC 
AGCCCCTATG ATAGGTATTT CCCTATACCA TCTCCTCTGT TCGGCTATTA CCTGCACACC 
AAATATCTGA ATGAGGTATT CCGCCGGCGT CATGATCTCT ATCCCTCGCC TCTGCAGCAC 
ACTCCATCTT CCATAGCCTC CGAAACGGAG ACTTCACCCA ATGCTCAGGA GCGCAAGAGT 
AGCTCCAACC ACGTCTTGCC CCACGCTCTG TTGGCCAATA ATTCACCTCC GCCATCGCTG 
CCATCGCCAC CGCGCAGCGA ATCCTCGGTG ACTAACAATG TGGCCACCAC CACCACTAGC 
AGCACCACCA AGAAGCGCAG CAGTCCCAAG CCCAAGGGCA AGAAGGGTGA GAAGAAGCCA 
ATGCCACCGC CGCAAGAGCG TCCCTTGGAT CTGTGCATGC GCAACGAAGT GGAGCCCAAG 
AAGTACAAAA AGTCAGGCTC GAAATCCTCT CTAGAGTCCC GTTCCGCCGG TATGATGCCG 
CCGCCGCCGG CTCTGAGTGC GGCCAGCAGT TTGGAGAGCA TGAGTGCCCT ATCACCGGCA 
TCGAGTAGCC ACTCGGGTCA CATGCCCATC ACCAGCGCTG CCACGCCCAA CCACCAGCCA 
CCACCCAATT CCCCCTATGC CAATGCCATG AACGCTGCCC ATGCAGCGGC AGCAGCGGCG 
GCGGCAGCCA TGATCAAGAT GGAGATGCCT TTGCATCCGC TGCACCACCA GCAGATGCAT 
CACTCGCAAG TGCCCACCAC CACAGTGGGT GTGCCGGTGA TCAAGGGCGA TGTGGCCTCG 
CCCACAACAA AGGAAACGGT CGCCTGGCGT TACAATCTGG ACGTGTCGCC CGTGGTTGAG 
GAGATGCCGC CCGGTTCGGA TGTGGCTTAT GTATGCCCCA CCTGCGGACA GATGTTCTCG 
CTGCACGACC GCCTGGCCAA GCACATGGCC TCGCGTCACA AGTCCCGCAA TCCCGCCAAT 
GATATTGCCA AAGCCTATTC CTGCGATGTG TGCCGGCGAT CCTTTGCCCG CTCCGATATG 
CTGACCCGCC ACATGCGCCT CCACACCGGA GTCAAGCCGT ACACGTGCAA GGTGTGCGGT 
CAGGTCTTTT CCCGCTCGGA TCACCTGTCC ACCCACCAGA GGACCCACAC CGGCGAGAAG 
CCCTACAAGT GTCCGCAGTG CCCGTATGCC GCCTGTCGTC GCGACATGAT CACCAGGCAT 
ATGCGGACAC ACACGCGGTA CGATTCCAGG GGAGGATCTC GAGAAGGCAG GGAAGGACGC 
GAAGGACGTG AGGGCAAGGA GAGCCGCGAT TCGCGCAGGA GCAACGAACG AATCGAGGAG 
CCCAACTCGC CCGGTTCCCA TTCGCTGCTG GACATGAAGA TGAACATGAA CATGGGCATG 
GGAATGGGCC TCAATATGGG CCTACCCATG AATCCGATGA GTCTACTGCA GGAGGAACTG 
CTGCAGAAGT CCCAGCCGGG CCTAACCCTG GGTGGACCCA TGCCACTGGT TGTCAAGACG 
GAGAGCGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

klu ( NM_001274762.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261691.1 Drosophila melanogaster klumpfuss (klu), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa18190
Clone ID Related Accession (Same CDS sequence)	NM_001300095.1 , NM_057714.4 , NM_001274762.1
Accession Version	NM_001274762.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	klumpfuss, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGATGG CAGAAGGCAC CGACCCCATG GGGCATGGTC ACCACACGAA TCCGCACAAT 
CCGCCCCAGC AGCCGCCGCA GCACCATCCG CACCACCTGC CCCTGCCCCA TCCCCCGCCG 
GCACCACCGC CGCAGCTACT CAATTTCAAT CACCACCAGC AGCAGTATCT TCCCCACATG 
TATCCTGCGG CAGCGGCAGC GGCACAATCT CACTCCCACT ACGGTGGCCT GCAGTATCCC 
CATCCACATG CGCTGCAGCA TCAGCATCAT CCCCACCAGC ACCAGCATCA CTTGCCGCTG 
GCCTTGAGCC TCCATCAGCA GGCGGCTGCG GCGGCGGCAG TGGCTGCAGC GGTGGCCACC 
AACCACTCCA ACAATAACAA TCAGGCCAGC GTGGCCAACA ATAATATAGT GGTTCCATGG 
AAGCAGCGCA AGCGCAAGAG GCTCTCAGCG GTGCTGGACA AGCTGCACCA CAATAATAAT 
AATAATAATA ACAACAATAA TGAGACCCAC TCCAATGGCA TGGCCTGCAA GGCGGAACCG 
ATGGAAATGA AGGGGGATCT AGCCGAAGAT GCCGAACAGG ATGAAGATCA TGATGGGTCC 
GTGCCTGATG AGGAAGATGA TTCCGTAGAT CAGGATGGTG ATGCTGGCAA GTACATCAAG 
AGCGAGCAGC CCGTCAGCGA GGACGAGGAG AACGAACAGG AAATGGACCA AGATATCGAG 
GACATTTCCG GCGAGGATCT CGAGCTGTCG CACAGCGATG ATAATGACCA GGACGACAGC 
CCCAGGATCA GTATGAGTCC TCTGCAGCTG CAGGCCGCCC AGAGCAATCT GCCCCAAGAT 
CAGAACCAGC CGATGAACAA GGAGAATCCG CTTCATGTGG ACATCAAGAC TGAGATCCCC 
AGCCCCTATG ATAGGTATTT CCCTATACCA TCTCCTCTGT TCGGCTATTA CCTGCACACC 
AAATATCTGA ATGAGGTATT CCGCCGGCGT CATGATCTCT ATCCCTCGCC TCTGCAGCAC 
ACTCCATCTT CCATAGCCTC CGAAACGGAG ACTTCACCCA ATGCTCAGGA GCGCAAGAGT 
AGCTCCAACC ACGTCTTGCC CCACGCTCTG TTGGCCAATA ATTCACCTCC GCCATCGCTG 
CCATCGCCAC CGCGCAGCGA ATCCTCGGTG ACTAACAATG TGGCCACCAC CACCACTAGC 
AGCACCACCA AGAAGCGCAG CAGTCCCAAG CCCAAGGGCA AGAAGGGTGA GAAGAAGCCA 
ATGCCACCGC CGCAAGAGCG TCCCTTGGAT CTGTGCATGC GCAACGAAGT GGAGCCCAAG 
AAGTACAAAA AGTCAGGCTC GAAATCCTCT CTAGAGTCCC GTTCCGCCGG TATGATGCCG 
CCGCCGCCGG CTCTGAGTGC GGCCAGCAGT TTGGAGAGCA TGAGTGCCCT ATCACCGGCA 
TCGAGTAGCC ACTCGGGTCA CATGCCCATC ACCAGCGCTG CCACGCCCAA CCACCAGCCA 
CCACCCAATT CCCCCTATGC CAATGCCATG AACGCTGCCC ATGCAGCGGC AGCAGCGGCG 
GCGGCAGCCA TGATCAAGAT GGAGATGCCT TTGCATCCGC TGCACCACCA GCAGATGCAT 
CACTCGCAAG TGCCCACCAC CACAGTGGGT GTGCCGGTGA TCAAGGGCGA TGTGGCCTCG 
CCCACAACAA AGGAAACGGT CGCCTGGCGT TACAATCTGG ACGTGTCGCC CGTGGTTGAG 
GAGATGCCGC CCGGTTCGGA TGTGGCTTAT GTATGCCCCA CCTGCGGACA GATGTTCTCG 
CTGCACGACC GCCTGGCCAA GCACATGGCC TCGCGTCACA AGTCCCGCAA TCCCGCCAAT 
GATATTGCCA AAGCCTATTC CTGCGATGTG TGCCGGCGAT CCTTTGCCCG CTCCGATATG 
CTGACCCGCC ACATGCGCCT CCACACCGGA GTCAAGCCGT ACACGTGCAA GGTGTGCGGT 
CAGGTCTTTT CCCGCTCGGA TCACCTGTCC ACCCACCAGA GGACCCACAC CGGCGAGAAG 
CCCTACAAGT GTCCGCAGTG CCCGTATGCC GCCTGTCGTC GCGACATGAT CACCAGGCAT 
ATGCGGACAC ACACGCGGTA CGATTCCAGG GGAGGATCTC GAGAAGGCAG GGAAGGACGC 
GAAGGACGTG AGGGCAAGGA GAGCCGCGAT TCGCGCAGGA GCAACGAACG AATCGAGGAG 
CCCAACTCGC CCGGTTCCCA TTCGCTGCTG GACATGAAGA TGAACATGAA CATGGGCATG 
GGAATGGGCC TCAATATGGG CCTACCCATG AATCCGATGA GTCTACTGCA GGAGGAACTG 
CTGCAGAAGT CCCAGCCGGG CCTAACCCTG GGTGGACCCA TGCCACTGGT TGTCAAGACG 
GAGAGCGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261691.1
CDS	707..3118
Translation	MTMAEGTDPMGHGHHTNPHNPPQQPPQHHPHHLPLPHPPPAPPPQLLNFNHHQQQYLPHMYPAAAAAAQSHSHYGGLQYPHPHALQHQHHPHQHQHHLPLALSLHQQAAAAAAVAAAVATNHSNNNNQASVANNNIVVPWKQRKRKRLSAVLDKLHHNNNNNNNNNNETHSNGMACKAEPMEMKGDLAEDAEQDEDHDGSVPDEEDDSVDQDGDAGKYIKSEQPVSEDEENEQEMDQDIEDISGEDLELSHSDDNDQDDSPRISMSPLQLQAAQSNLPQDQNQPMNKENPLHVDIKTEIPSPYDRYFPIPSPLFGYYLHTKYLNEVFRRRHDLYPSPLQHTPSSIASETETSPNAQERKSSSNHVLPHALLANNSPPPSLPSPPRSESSVTNNVATTTTSSTTKKRSSPKPKGKKGEKKPMPPPQERPLDLCMRNEVEPKKYKKSGSKSSLESRSAGMMPPPPALSAASSLESMSALSPASSSHSGHMPITSAATPNHQPPPNSPYANAMNAAHAAAAAAAAAMIKMEMPLHPLHHQQMHHSQVPTTTVGVPVIKGDVASPTTKETVAWRYNLDVSPVVEEMPPGSDVAYVCPTCGQMFSLHDRLAKHMASRHKSRNPANDIAKAYSCDVCRRSFARSDMLTRHMRLHTGVKPYTCKVCGQVFSRSDHLSTHQRTHTGEKPYKCPQCPYAACRRDMITRHMRTHTRYDSRGGSREGREGREGREGKESRDSRRSNERIEEPNSPGSHSLLDMKMNMNMGMGMGLNMGLPMNPMSLLQEELLQKSQPGLTLGGPMPLVVKTESA

Target ORF information:

RefSeq Version	NM_001274762.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster klumpfuss (klu), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274762.1

ORF Insert Sequence:

ATGACGATGG CAGAAGGCAC CGACCCCATG GGGCATGGTC ACCACACGAA TCCGCACAAT 
CCGCCCCAGC AGCCGCCGCA GCACCATCCG CACCACCTGC CCCTGCCCCA TCCCCCGCCG 
GCACCACCGC CGCAGCTACT CAATTTCAAT CACCACCAGC AGCAGTATCT TCCCCACATG 
TATCCTGCGG CAGCGGCAGC GGCACAATCT CACTCCCACT ACGGTGGCCT GCAGTATCCC 
CATCCACATG CGCTGCAGCA TCAGCATCAT CCCCACCAGC ACCAGCATCA CTTGCCGCTG 
GCCTTGAGCC TCCATCAGCA GGCGGCTGCG GCGGCGGCAG TGGCTGCAGC GGTGGCCACC 
AACCACTCCA ACAATAACAA TCAGGCCAGC GTGGCCAACA ATAATATAGT GGTTCCATGG 
AAGCAGCGCA AGCGCAAGAG GCTCTCAGCG GTGCTGGACA AGCTGCACCA CAATAATAAT 
AATAATAATA ACAACAATAA TGAGACCCAC TCCAATGGCA TGGCCTGCAA GGCGGAACCG 
ATGGAAATGA AGGGGGATCT AGCCGAAGAT GCCGAACAGG ATGAAGATCA TGATGGGTCC 
GTGCCTGATG AGGAAGATGA TTCCGTAGAT CAGGATGGTG ATGCTGGCAA GTACATCAAG 
AGCGAGCAGC CCGTCAGCGA GGACGAGGAG AACGAACAGG AAATGGACCA AGATATCGAG 
GACATTTCCG GCGAGGATCT CGAGCTGTCG CACAGCGATG ATAATGACCA GGACGACAGC 
CCCAGGATCA GTATGAGTCC TCTGCAGCTG CAGGCCGCCC AGAGCAATCT GCCCCAAGAT 
CAGAACCAGC CGATGAACAA GGAGAATCCG CTTCATGTGG ACATCAAGAC TGAGATCCCC 
AGCCCCTATG ATAGGTATTT CCCTATACCA TCTCCTCTGT TCGGCTATTA CCTGCACACC 
AAATATCTGA ATGAGGTATT CCGCCGGCGT CATGATCTCT ATCCCTCGCC TCTGCAGCAC 
ACTCCATCTT CCATAGCCTC CGAAACGGAG ACTTCACCCA ATGCTCAGGA GCGCAAGAGT 
AGCTCCAACC ACGTCTTGCC CCACGCTCTG TTGGCCAATA ATTCACCTCC GCCATCGCTG 
CCATCGCCAC CGCGCAGCGA ATCCTCGGTG ACTAACAATG TGGCCACCAC CACCACTAGC 
AGCACCACCA AGAAGCGCAG CAGTCCCAAG CCCAAGGGCA AGAAGGGTGA GAAGAAGCCA 
ATGCCACCGC CGCAAGAGCG TCCCTTGGAT CTGTGCATGC GCAACGAAGT GGAGCCCAAG 
AAGTACAAAA AGTCAGGCTC GAAATCCTCT CTAGAGTCCC GTTCCGCCGG TATGATGCCG 
CCGCCGCCGG CTCTGAGTGC GGCCAGCAGT TTGGAGAGCA TGAGTGCCCT ATCACCGGCA 
TCGAGTAGCC ACTCGGGTCA CATGCCCATC ACCAGCGCTG CCACGCCCAA CCACCAGCCA 
CCACCCAATT CCCCCTATGC CAATGCCATG AACGCTGCCC ATGCAGCGGC AGCAGCGGCG 
GCGGCAGCCA TGATCAAGAT GGAGATGCCT TTGCATCCGC TGCACCACCA GCAGATGCAT 
CACTCGCAAG TGCCCACCAC CACAGTGGGT GTGCCGGTGA TCAAGGGCGA TGTGGCCTCG 
CCCACAACAA AGGAAACGGT CGCCTGGCGT TACAATCTGG ACGTGTCGCC CGTGGTTGAG 
GAGATGCCGC CCGGTTCGGA TGTGGCTTAT GTATGCCCCA CCTGCGGACA GATGTTCTCG 
CTGCACGACC GCCTGGCCAA GCACATGGCC TCGCGTCACA AGTCCCGCAA TCCCGCCAAT 
GATATTGCCA AAGCCTATTC CTGCGATGTG TGCCGGCGAT CCTTTGCCCG CTCCGATATG 
CTGACCCGCC ACATGCGCCT CCACACCGGA GTCAAGCCGT ACACGTGCAA GGTGTGCGGT 
CAGGTCTTTT CCCGCTCGGA TCACCTGTCC ACCCACCAGA GGACCCACAC CGGCGAGAAG 
CCCTACAAGT GTCCGCAGTG CCCGTATGCC GCCTGTCGTC GCGACATGAT CACCAGGCAT 
ATGCGGACAC ACACGCGGTA CGATTCCAGG GGAGGATCTC GAGAAGGCAG GGAAGGACGC 
GAAGGACGTG AGGGCAAGGA GAGCCGCGAT TCGCGCAGGA GCAACGAACG AATCGAGGAG 
CCCAACTCGC CCGGTTCCCA TTCGCTGCTG GACATGAAGA TGAACATGAA CATGGGCATG 
GGAATGGGCC TCAATATGGG CCTACCCATG AATCCGATGA GTCTACTGCA GGAGGAACTG 
CTGCAGAAGT CCCAGCCGGG CCTAACCCTG GGTGGACCCA TGCCACTGGT TGTCAAGACG 
GAGAGCGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

klu ( NM_001274761.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261690.1 Drosophila melanogaster klumpfuss (klu), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa19896
Clone ID Related Accession (Same CDS sequence)	NM_001274761.1
Accession Version	NM_001274761.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	klumpfuss, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGATGG CAGAAGGCAC CGACCCCATG GGGCATGGTC ACCACACGAA TCCGCACAAT 
CCGCCCCAGC AGCCGCCGCA GCACCATCCG CACCACCTGC CCCTGCCCCA TCCCCCGCCG 
GCACCACCGC CGCAGCTACT CAATTTCAAT CACCACCAGC AGCAGTATCT TCCCCACATG 
TATCCTGCGG CAGCGGCAGC GGCACAATCT CACTCCCACT ACGGTGGCCT GCAGTATCCC 
CATCCACATG CGCTGCAGCA TCAGCATCAT CCCCACCAGC ACCAGCATCA CTTGCCGCTG 
GCCTTGAGCC TCCATCAGCA GGCGGCTGCG GCGGCGGCAG TGGCTGCAGC GGTGGCCACC 
AACCACTCCA ACAATAACAA TCAGGCCAGC GTGGCCAACA ATAATATAGT GGTTCCATGG 
AAGCAGCGCA AGCGCAAGAG GCTCTCAGCG GTGCTGGACA AGCTGCACCA CAATAATAAT 
AATAATAATA ACAACAATAA TGAGACCCAC TCCAATGGCA TGGCCTGCAA GGCGGAACCG 
ATGGAAATGA AGGGGGATCT AGCCGAAGAT GCCGAACAGG ATGAAGATCA TGATGGGTCC 
GTGCCTGATG AGGAAGATGA TTCCGTAGAT CAGGATGGTG ATGCTGGCAA GTACATCAAG 
AGCGAGCAGC CCGTCAGCGA GGACGAGGAG AACGAACAGG AAATGGACCA AGATATCGAG 
GACATTTCCG GCGAGGATCT CGAGCTGTCG CACAGCGATG ATAATGACCA GGACGACAGC 
CCCAGGATCA GTATGAGTCC TCTGCAGCTG CAGGCCGCCC AGAGCAATCT GCCCCAAGAT 
CAGAACCAGC CGATGAACAA GGAGAATCCG CTTCATGTGG ACATCAAGAC TGAGATCCCC 
AGCCCCTATG ATAGGTATTT CCCTATACCA TCTCCTCTGT TCGGCTATTA CCTGCACACC 
AAATATCTGA ATGAGGTATT CCGCCGGCGT CATGATCTCT ATCCCTCGCC TCTGCAGCAC 
ACTCCATCTT CCATAGCCTC CGAAACGGAG ACTTCACCCA ATGCTCAGGA GCGCAAGAGT 
AGCTCCAACC ACGTCTTGCC CCACGCTCTG TTGGCCAATA ATTCACCTCC GCCATCGCTG 
CCATCGCCAC CGCGCAGCGA ATCCTCGGTG ACTAACAATG TGGCCACCAC CACCACTAGC 
AGCACCACCA AGAAGCGCAG CAGTCCCAAG CCCAAGGGCA AGAAGGGTGA GAAGAAGCCA 
ATGCCACCGC CGCAAGAGCG TCCCTTGGAT CTGTGCATGC GCAACGAAGT GGAGCCCAAG 
AAGTACAAAA AGTCAGGCTC GAAATCCTCT CTAGAGTCCC GTTCCGCCGG TATGATGCCG 
CCGCCGCCGG CTCTGAGTGC GGCCAGCAGT TTGGAGAGCA TGAGTGCCCT ATCACCGGCA 
TCGAGTAGCC ACTCGGGTCA CATGCCCATC ACCAGCGCTG CCACGCCCAA CCACCAGCCA 
CCACCCAATT CCCCCTATGC CAATGCCATG AACGCTGCCC ATGCAGCGGC AGCAGCGGCG 
GCGGCAGCCA TGATCAAGAT GGAGATGCCT TTGCATCCGC TGCACCACCA GCAGATGCAT 
CACTCGCAAG TGCCCACCAC CACAGTGGGT GTGCCGGTGA TCAAGGGCGA TGTGGCCTCG 
CCCACAACAA AGGAAACGGT CGCCTGGCGT TACAATCTGG ACGTGTCGCC CGTGGTTGAG 
GAGATGCCGC CCGGTTCGGA TGTGGCTTAT GTATGCCCCA CCTGCGGACA GATGTTCTCG 
CTGCACGACC GCCTGGCCAA GCACATGGCC TCGCGTCACA AGTCCCGCAA TCCCGCCAAT 
GATATTGCCA AAGCCTATTC CTGCGATGTG TGCCGGCGAT CCTTTGCCCG CTCCGATATG 
CTGACCCGCC ACATGCGCCT CCACACCGGA GTCAAGCCGT ACACGTGCAA GGTGTGCGGT 
CAGGTCTTTT CCCGCTCGGA TCACCTGTCC ACCCACCAGA GGACCCACAC CGGCGAGAAG 
CCCTACAAGT GTCCGCAGTG CCCGTATGCC GCCTGTCGTC GCGACATGAT CACCAGGCAT 
ATGCGGACAC ACACGCGGTA CGATTCCAGG GGAGGATCTC GAGAAGGCAG GGAAGGACGC 
GAAGGACGTG AGGGCAAGGA GAGCCGCGAT TCGCGCAGGA GCAACGAACG AATCGAGGAG 
CCCAACTCGC CCGGTTCCCA TTCGCTGCTG GACATGAAGA TGAACATGAA CATGGGCATG 
GGAATGGGCC TCAATATGGG CCTACCCATG AATCCGATGA GTCTACTGCA GGAGGAACTG 
CTGCAGAAGT CCCAGCCGGG CCTAACCCTG GGTGGACCCA TGCCACTGGT TGTCAAGACG 
GAGAGCGCCT AACGGTGTCT GTATGCAGCA GCATCCAGAC CATTTATGAC CTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261690.1
CDS	707..3163
Translation	MTMAEGTDPMGHGHHTNPHNPPQQPPQHHPHHLPLPHPPPAPPPQLLNFNHHQQQYLPHMYPAAAAAAQSHSHYGGLQYPHPHALQHQHHPHQHQHHLPLALSLHQQAAAAAAVAAAVATNHSNNNNQASVANNNIVVPWKQRKRKRLSAVLDKLHHNNNNNNNNNNETHSNGMACKAEPMEMKGDLAEDAEQDEDHDGSVPDEEDDSVDQDGDAGKYIKSEQPVSEDEENEQEMDQDIEDISGEDLELSHSDDNDQDDSPRISMSPLQLQAAQSNLPQDQNQPMNKENPLHVDIKTEIPSPYDRYFPIPSPLFGYYLHTKYLNEVFRRRHDLYPSPLQHTPSSIASETETSPNAQERKSSSNHVLPHALLANNSPPPSLPSPPRSESSVTNNVATTTTSSTTKKRSSPKPKGKKGEKKPMPPPQERPLDLCMRNEVEPKKYKKSGSKSSLESRSAGMMPPPPALSAASSLESMSALSPASSSHSGHMPITSAATPNHQPPPNSPYANAMNAAHAAAAAAAAAMIKMEMPLHPLHHQQMHHSQVPTTTVGVPVIKGDVASPTTKETVAWRYNLDVSPVVEEMPPGSDVAYVCPTCGQMFSLHDRLAKHMASRHKSRNPANDIAKAYSCDVCRRSFARSDMLTRHMRLHTGVKPYTCKVCGQVFSRSDHLSTHQRTHTGEKPYKCPQCPYAACRRDMITRHMRTHTRYDSRGGSREGREGREGREGKESRDSRRSNERIEEPNSPGSHSLLDMKMNMNMGMGMGLNMGLPMNPMSLLQEELLQKSQPGLTLGGPMPLVVKTESA*RCLYAAASRPFMTC

Target ORF information:

RefSeq Version	NM_001274761.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster klumpfuss (klu), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274761.1

ORF Insert Sequence:

ATGACGATGG CAGAAGGCAC CGACCCCATG GGGCATGGTC ACCACACGAA TCCGCACAAT 
CCGCCCCAGC AGCCGCCGCA GCACCATCCG CACCACCTGC CCCTGCCCCA TCCCCCGCCG 
GCACCACCGC CGCAGCTACT CAATTTCAAT CACCACCAGC AGCAGTATCT TCCCCACATG 
TATCCTGCGG CAGCGGCAGC GGCACAATCT CACTCCCACT ACGGTGGCCT GCAGTATCCC 
CATCCACATG CGCTGCAGCA TCAGCATCAT CCCCACCAGC ACCAGCATCA CTTGCCGCTG 
GCCTTGAGCC TCCATCAGCA GGCGGCTGCG GCGGCGGCAG TGGCTGCAGC GGTGGCCACC 
AACCACTCCA ACAATAACAA TCAGGCCAGC GTGGCCAACA ATAATATAGT GGTTCCATGG 
AAGCAGCGCA AGCGCAAGAG GCTCTCAGCG GTGCTGGACA AGCTGCACCA CAATAATAAT 
AATAATAATA ACAACAATAA TGAGACCCAC TCCAATGGCA TGGCCTGCAA GGCGGAACCG 
ATGGAAATGA AGGGGGATCT AGCCGAAGAT GCCGAACAGG ATGAAGATCA TGATGGGTCC 
GTGCCTGATG AGGAAGATGA TTCCGTAGAT CAGGATGGTG ATGCTGGCAA GTACATCAAG 
AGCGAGCAGC CCGTCAGCGA GGACGAGGAG AACGAACAGG AAATGGACCA AGATATCGAG 
GACATTTCCG GCGAGGATCT CGAGCTGTCG CACAGCGATG ATAATGACCA GGACGACAGC 
CCCAGGATCA GTATGAGTCC TCTGCAGCTG CAGGCCGCCC AGAGCAATCT GCCCCAAGAT 
CAGAACCAGC CGATGAACAA GGAGAATCCG CTTCATGTGG ACATCAAGAC TGAGATCCCC 
AGCCCCTATG ATAGGTATTT CCCTATACCA TCTCCTCTGT TCGGCTATTA CCTGCACACC 
AAATATCTGA ATGAGGTATT CCGCCGGCGT CATGATCTCT ATCCCTCGCC TCTGCAGCAC 
ACTCCATCTT CCATAGCCTC CGAAACGGAG ACTTCACCCA ATGCTCAGGA GCGCAAGAGT 
AGCTCCAACC ACGTCTTGCC CCACGCTCTG TTGGCCAATA ATTCACCTCC GCCATCGCTG 
CCATCGCCAC CGCGCAGCGA ATCCTCGGTG ACTAACAATG TGGCCACCAC CACCACTAGC 
AGCACCACCA AGAAGCGCAG CAGTCCCAAG CCCAAGGGCA AGAAGGGTGA GAAGAAGCCA 
ATGCCACCGC CGCAAGAGCG TCCCTTGGAT CTGTGCATGC GCAACGAAGT GGAGCCCAAG 
AAGTACAAAA AGTCAGGCTC GAAATCCTCT CTAGAGTCCC GTTCCGCCGG TATGATGCCG 
CCGCCGCCGG CTCTGAGTGC GGCCAGCAGT TTGGAGAGCA TGAGTGCCCT ATCACCGGCA 
TCGAGTAGCC ACTCGGGTCA CATGCCCATC ACCAGCGCTG CCACGCCCAA CCACCAGCCA 
CCACCCAATT CCCCCTATGC CAATGCCATG AACGCTGCCC ATGCAGCGGC AGCAGCGGCG 
GCGGCAGCCA TGATCAAGAT GGAGATGCCT TTGCATCCGC TGCACCACCA GCAGATGCAT 
CACTCGCAAG TGCCCACCAC CACAGTGGGT GTGCCGGTGA TCAAGGGCGA TGTGGCCTCG 
CCCACAACAA AGGAAACGGT CGCCTGGCGT TACAATCTGG ACGTGTCGCC CGTGGTTGAG 
GAGATGCCGC CCGGTTCGGA TGTGGCTTAT GTATGCCCCA CCTGCGGACA GATGTTCTCG 
CTGCACGACC GCCTGGCCAA GCACATGGCC TCGCGTCACA AGTCCCGCAA TCCCGCCAAT 
GATATTGCCA AAGCCTATTC CTGCGATGTG TGCCGGCGAT CCTTTGCCCG CTCCGATATG 
CTGACCCGCC ACATGCGCCT CCACACCGGA GTCAAGCCGT ACACGTGCAA GGTGTGCGGT 
CAGGTCTTTT CCCGCTCGGA TCACCTGTCC ACCCACCAGA GGACCCACAC CGGCGAGAAG 
CCCTACAAGT GTCCGCAGTG CCCGTATGCC GCCTGTCGTC GCGACATGAT CACCAGGCAT 
ATGCGGACAC ACACGCGGTA CGATTCCAGG GGAGGATCTC GAGAAGGCAG GGAAGGACGC 
GAAGGACGTG AGGGCAAGGA GAGCCGCGAT TCGCGCAGGA GCAACGAACG AATCGAGGAG 
CCCAACTCGC CCGGTTCCCA TTCGCTGCTG GACATGAAGA TGAACATGAA CATGGGCATG 
GGAATGGGCC TCAATATGGG CCTACCCATG AATCCGATGA GTCTACTGCA GGAGGAACTG 
CTGCAGAAGT CCCAGCCGGG CCTAACCCTG GGTGGACCCA TGCCACTGGT TGTCAAGACG 
GAGAGCGCCT AACGGTGTCT GTATGCAGCA GCATCCAGAC CATTTATGAC CTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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klu cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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