E(z) cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	E(z)
Entrez Gene ID	39203
Full Name	Enhancer of zeste
Synonyms	CG6502,Dmel\CG6502,E(Z),E(z)1,EZ,EZH1/2,E[z],Ez,Su(z)301,l(3)1902,l(3)67Fa,l(3)B12,l(3)SG17,l(3)ds12,pco,pco/E(z)
General protein information	Preferred Names Enhancer of zeste Names CG6502 gene product from transcript CG6502-RA CG6502-PA CG6502-PB CG6502-PC E(z)-PA E(z)-PB E(z)-PC enhancer of zest enhancer of zeste enhancer-of-zeste polycombeotic
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 67E5-67E5\|3-34.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001144460.3	NP_001137932.1	enhancer of zeste, isoform B
NM_001274753.1	NP_001261682.1	enhancer of zeste, isoform C
NM_079297.3	NP_524021.2	enhancer of zeste, isoform A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EZH2	XP_001166174.2
Mus musculus (house mouse)	Ezh2	NP_031997.2
Gallus gallus (chicken)	EZH2	XP_418879.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012516	XP_307419.2
Homo sapiens (human)	EZH2	NP_004447.2
Rattus norvegicus (Norway rat)	Ezh2	NP_001128451.1
	EZH2	XP_002803555.1
Canis lupus familiaris (dog)	EZH2	XP_005629569.1
Drosophila melanogaster (fruit fly)	E(z)	NP_001261682.1
Bos taurus (cattle)	EZH2	NP_001179953.1
Danio rerio (zebrafish)	ezh2	NP_001070747.1
Xenopus tropicalis (tropical clawed frog)	ezh2	NP_001017293.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Taken together, these data suggest that E(z) acts intrinsically in germ cells to activate dedifferentiation and thus replenish lost germline stem cells during both aging and tissue regeneration.
Title: Polycomb Group Gene E(z) Is Required for Spermatogonial Dedifferentiation in Drosophila Adult Testis.

Chracterization of functionally critical SU(Z)12 elements, including key determinants of SU(Z)12-E(Z) communication.
Title: Elements of the polycomb repressor SU(Z)12 needed for histone H3-K27 methylation, the interface with E(Z), and in vivo function.

The emerging picture presents PRC2 as a cooperative multipart machine, intricately outfitted to sense and respond to the local chromatin environment and other cues[review]
Title: Inner workings and regulatory inputs that control Polycomb repressive complex 2.

These results suggest that increased H3K27 trimethylation activity of E(Z)(Trm) causes the premature accumulation of H3K27me3 in early embryogenesis, "predestining" initially active Polycomb target genes to silencing once Polycomb silencing is initiated.
Title: A mutation in the E(Z) methyltransferase that increases trimethylation of histone H3 lysine 27 and causes inappropriate silencing of active Polycomb target genes.

A Pcl-PSC2 complex is the histone methyltransferase that generates the high levels of histone3-lysine27 trimethylation in Polycomb target genes that are needed to maintain a Polycomb-repressed chromatin state.
Title: Pcl-PRC2 is needed to generate high levels of H3-K27 trimethylation at Polycomb target genes.

The following E(z) gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the E(z) cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18161	NM_001144460.3 Latest version!	Drosophila melanogaster enhancer of zeste, transcript variant B (E(z)), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa18161	NM_079297.3 Latest version!	Drosophila melanogaster enhancer of zeste, transcript variant A (E(z)), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa19891	NM_001274753.1 Latest version!	Drosophila melanogaster enhancer of zeste, transcript variant C (E(z)), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

E(z) ( NM_001144460.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137932.1 Drosophila melanogaster enhancer of zeste, transcript variant B (E(z)), mRNA.

Price & Availability↑

CloneID	OFa18161
Clone ID Related Accession (Same CDS sequence)	NM_001144460.3 , NM_079297.3
Accession Version	NM_001144460.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of zeste, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001144460.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATAGCA CTAAAGTGCC GCCCGAGTGG AAGAGGCGTG TCAAGTCGGA GTACATCAAA 
ATCAGGCAGC AGAAGCGCTA CAAACGTGCC GACGAGATCA AGGAGGCGTG GATAAGAAAC 
TGGGACGAGC ACAACCACAA TGTACAGGAT CTGTACTGCG AGTCGAAGGT TTGGCAGGCT 
AAACCCTACG ATCCGCCGCA TGTGGACTGT GTGAAGCGGG CGGAGGTGAC TAGCTACAAT 
GGCATTCCCA GCGGTCCGCA AAAGGTGCCC ATTTGCGTGA TCAACGCGGT CACTCCCATA 
CCCACCATGT ACACGTGGGC ACCAACACAG CAGAACTTCA TGGTGGAGGA TGAGACGGTG 
CTGCACAATA TACCCTATAT GGGCGACGAG GTCCTCGATA AGGATGGCAA GTTCATAGAG 
GAACTGATCA AAAACTACGA TGGCAAGGTT CACGGCGACA AGGATCCCTC GTTCATGGAC 
GATGCCATCT TCGTAGAACT GGTGCACGCC CTTATGCGCT CCTACAGCAA GGAACTGGAG 
GAAGCCGCTC CGGGTACAGC GACTGCCATT AAAACAGAGA CGTTGGCGAA ATCAAAACAG 
GGCGAAGATG ATGGGGTAGT TGATGTTGAC GCAGATGGTG AATCTCCCAT GAAGCTTGAG 
AAAACCGACA GCAAAGGGGA TCTGACTGAA GTTGAGAAGA AGGAAACTGA AGAACCGCTG 
GAGACTGAAG ATGCGGATGT TAAGCCCGAT GTGGAAGAAG TCAAGGACAA GCTGCCCTTC 
CCTGCGCCCA TTATTTTTCA GGCCATCAGC GCTAACTTTC CAGACAAGGG CACCGCGCAA 
GAGCTCAAAG AAAAGTACAT TGAGCTTACG GAGCACCAGG ATCCGGAGCG CCCACAGGAG 
TGCACACCCA ACATTGACGG CATCAAGGCC GAGAGCGTTT CCCGGGAAAG GACTATGCAC 
TCCTTTCACA CGCTCTTCTG TCGTCGTTGC TTCAAATACG ACTGCTTCCT GCACCGCTTG 
CAAGGACATG CGGGTCCCAA TCTCCAGAAG CGTCGCTATC CGGAACTGAA GCCTTTCGCC 
GAACCTTGCA GCAACTCCTG CTACATGTTG ATCGATGGCA TGAAGGAAAA GCTGGCGGCC 
GATAGCAAGA CACCTCCCAT TGATTCCTGC AATGAGGCCA GCTCCGAGGA CAGTAATGAC 
AGCAACAGTC AGTTTAGCAA CAAGGACTTT AATCATGAGA ATAGCAAGGA TAATGGCCTG 
ACTGTAAATA GCGCCGCTGT GGCTGAGATC AACTCCATTA TGGCTGGCAT GATGAACATC 
ACCAGTACGC AGTGCGTTTG GACAGGCGCT GACCAAGCAC TTTATCGGGT CTTGCATAAA 
GTGTATTTAA AGAACTACTG CGCGATTGCG CACAACATGC TGACCAAGAC CTGTCGTCAG 
GTGTACGAGT TTGCCCAGAA GGAGGATGCT GAGTTTAGCT TCGAGGATTT GCGACAGGAC 
TTCACACCTC CGCGCAAGAA GAAGAAGAAG CAACGTCTGT GGTCGCTGCA CTGTCGTAAG 
ATTCAGCTAA AGAAGGACTC ATCCTCGAAT CACGTGTACA ACTACACGCC CTGCGACCAT 
CCCGGACATC CATGCGACAT GAACTGCTCC TGCATTCAGA CACAAAACTT CTGCGAAAAG 
TTCTGCAACT GCAGCAGCGA TTGCCAGAAT CGTTTCCCCG GCTGTCGCTG CAAGGCTCAG 
TGCAATACGA AGCAGTGTCC TTGCTATCTG GCAGTGCGCG AGTGTGATCC CGATCTGTGC 
CAGGCCTGCG GAGCGGATCA GTTTAAGCTG ACCAAGATCA CGTGCAAGAA CGTGTGCGTG 
CAGCGAGGAC TGCACAAGCA CCTGCTCATG GCTCCGTCTG ACATCGCCGG CTGGGGCATC 
TTTCTTAAGG AGGGTGCGCA AAAGAATGAG TTCATTTCGG AATATTGTGG CGAGATCATA 
TCCCAAGATG AAGCCGATCG CCGTGGCAAG GTTTACGACA AGTACATGTG CTCATTTCTT 
TTCAATCTGA ACAACGATTT TGTTGTGGAT GCCACTCGGA AGGGCAACAA GATTCGGTTT 
GCCAATCATT CCATTAATCC CAATTGCTAT GCCAAGGTTA TGATGGTCAC CGGCGATCAT 
CGCATCGGCA TCTTTGCCAA GCGGGCCATT CAGCCCGGCG AAGAGCTATT CTTTGACTAC 
AGATACGGAC CCACGGAGCA GCTGAAGTTC GTGGGCATAG AGCGTGAAAT GGAAATTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137932.1
CDS	119..2401
Translation	MNSTKVPPEWKRRVKSEYIKIRQQKRYKRADEIKEAWIRNWDEHNHNVQDLYCESKVWQAKPYDPPHVDCVKRAEVTSYNGIPSGPQKVPICVINAVTPIPTMYTWAPTQQNFMVEDETVLHNIPYMGDEVLDKDGKFIEELIKNYDGKVHGDKDPSFMDDAIFVELVHALMRSYSKELEEAAPGTATAIKTETLAKSKQGEDDGVVDVDADGESPMKLEKTDSKGDLTEVEKKETEEPLETEDADVKPDVEEVKDKLPFPAPIIFQAISANFPDKGTAQELKEKYIELTEHQDPERPQECTPNIDGIKAESVSRERTMHSFHTLFCRRCFKYDCFLHRLQGHAGPNLQKRRYPELKPFAEPCSNSCYMLIDGMKEKLAADSKTPPIDSCNEASSEDSNDSNSQFSNKDFNHENSKDNGLTVNSAAVAEINSIMAGMMNITSTQCVWTGADQALYRVLHKVYLKNYCAIAHNMLTKTCRQVYEFAQKEDAEFSFEDLRQDFTPPRKKKKKQRLWSLHCRKIQLKKDSSSNHVYNYTPCDHPGHPCDMNCSCIQTQNFCEKFCNCSSDCQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCQACGADQFKLTKITCKNVCVQRGLHKHLLMAPSDIAGWGIFLKEGAQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSINPNCYAKVMMVTGDHRIGIFAKRAIQPGEELFFDYRYGPTEQLKFVGIEREMEIV

Target ORF information:

RefSeq Version	NM_001144460.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of zeste, transcript variant B (E(z)), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144460.3

ORF Insert Sequence:

ATGAATAGCA CTAAAGTGCC GCCCGAGTGG AAGAGGCGTG TCAAGTCGGA GTACATCAAA 
ATCAGGCAGC AGAAGCGCTA CAAACGTGCC GACGAGATCA AGGAGGCGTG GATAAGAAAC 
TGGGACGAGC ACAACCACAA TGTACAGGAT CTGTACTGCG AGTCGAAGGT TTGGCAGGCT 
AAACCCTACG ATCCGCCGCA TGTGGACTGT GTGAAGCGGG CGGAGGTGAC TAGCTACAAT 
GGCATTCCCA GCGGTCCGCA AAAGGTGCCC ATTTGCGTGA TCAACGCGGT CACTCCCATA 
CCCACCATGT ACACGTGGGC ACCAACACAG CAGAACTTCA TGGTGGAGGA TGAGACGGTG 
CTGCACAATA TACCCTATAT GGGCGACGAG GTCCTCGATA AGGATGGCAA GTTCATAGAG 
GAACTGATCA AAAACTACGA TGGCAAGGTT CACGGCGACA AGGATCCCTC GTTCATGGAC 
GATGCCATCT TCGTAGAACT GGTGCACGCC CTTATGCGCT CCTACAGCAA GGAACTGGAG 
GAAGCCGCTC CGGGTACAGC GACTGCCATT AAAACAGAGA CGTTGGCGAA ATCAAAACAG 
GGCGAAGATG ATGGGGTAGT TGATGTTGAC GCAGATGGTG AATCTCCCAT GAAGCTTGAG 
AAAACCGACA GCAAAGGGGA TCTGACTGAA GTTGAGAAGA AGGAAACTGA AGAACCGCTG 
GAGACTGAAG ATGCGGATGT TAAGCCCGAT GTGGAAGAAG TCAAGGACAA GCTGCCCTTC 
CCTGCGCCCA TTATTTTTCA GGCCATCAGC GCTAACTTTC CAGACAAGGG CACCGCGCAA 
GAGCTCAAAG AAAAGTACAT TGAGCTTACG GAGCACCAGG ATCCGGAGCG CCCACAGGAG 
TGCACACCCA ACATTGACGG CATCAAGGCC GAGAGCGTTT CCCGGGAAAG GACTATGCAC 
TCCTTTCACA CGCTCTTCTG TCGTCGTTGC TTCAAATACG ACTGCTTCCT GCACCGCTTG 
CAAGGACATG CGGGTCCCAA TCTCCAGAAG CGTCGCTATC CGGAACTGAA GCCTTTCGCC 
GAACCTTGCA GCAACTCCTG CTACATGTTG ATCGATGGCA TGAAGGAAAA GCTGGCGGCC 
GATAGCAAGA CACCTCCCAT TGATTCCTGC AATGAGGCCA GCTCCGAGGA CAGTAATGAC 
AGCAACAGTC AGTTTAGCAA CAAGGACTTT AATCATGAGA ATAGCAAGGA TAATGGCCTG 
ACTGTAAATA GCGCCGCTGT GGCTGAGATC AACTCCATTA TGGCTGGCAT GATGAACATC 
ACCAGTACGC AGTGCGTTTG GACAGGCGCT GACCAAGCAC TTTATCGGGT CTTGCATAAA 
GTGTATTTAA AGAACTACTG CGCGATTGCG CACAACATGC TGACCAAGAC CTGTCGTCAG 
GTGTACGAGT TTGCCCAGAA GGAGGATGCT GAGTTTAGCT TCGAGGATTT GCGACAGGAC 
TTCACACCTC CGCGCAAGAA GAAGAAGAAG CAACGTCTGT GGTCGCTGCA CTGTCGTAAG 
ATTCAGCTAA AGAAGGACTC ATCCTCGAAT CACGTGTACA ACTACACGCC CTGCGACCAT 
CCCGGACATC CATGCGACAT GAACTGCTCC TGCATTCAGA CACAAAACTT CTGCGAAAAG 
TTCTGCAACT GCAGCAGCGA TTGCCAGAAT CGTTTCCCCG GCTGTCGCTG CAAGGCTCAG 
TGCAATACGA AGCAGTGTCC TTGCTATCTG GCAGTGCGCG AGTGTGATCC CGATCTGTGC 
CAGGCCTGCG GAGCGGATCA GTTTAAGCTG ACCAAGATCA CGTGCAAGAA CGTGTGCGTG 
CAGCGAGGAC TGCACAAGCA CCTGCTCATG GCTCCGTCTG ACATCGCCGG CTGGGGCATC 
TTTCTTAAGG AGGGTGCGCA AAAGAATGAG TTCATTTCGG AATATTGTGG CGAGATCATA 
TCCCAAGATG AAGCCGATCG CCGTGGCAAG GTTTACGACA AGTACATGTG CTCATTTCTT 
TTCAATCTGA ACAACGATTT TGTTGTGGAT GCCACTCGGA AGGGCAACAA GATTCGGTTT 
GCCAATCATT CCATTAATCC CAATTGCTAT GCCAAGGTTA TGATGGTCAC CGGCGATCAT 
CGCATCGGCA TCTTTGCCAA GCGGGCCATT CAGCCCGGCG AAGAGCTATT CTTTGACTAC 
AGATACGGAC CCACGGAGCA GCTGAAGTTC GTGGGCATAG AGCGTGAAAT GGAAATTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E(z) ( NM_079297.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524021.2 Drosophila melanogaster enhancer of zeste, transcript variant A (E(z)), mRNA.

Price & Availability↑

CloneID	OFa18161
Clone ID Related Accession (Same CDS sequence)	NM_001144460.3 , NM_079297.3
Accession Version	NM_079297.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of zeste, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Dec 17, 2009 this sequence version replaced NM_079297.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATAGCA CTAAAGTGCC GCCCGAGTGG AAGAGGCGTG TCAAGTCGGA GTACATCAAA 
ATCAGGCAGC AGAAGCGCTA CAAACGTGCC GACGAGATCA AGGAGGCGTG GATAAGAAAC 
TGGGACGAGC ACAACCACAA TGTACAGGAT CTGTACTGCG AGTCGAAGGT TTGGCAGGCT 
AAACCCTACG ATCCGCCGCA TGTGGACTGT GTGAAGCGGG CGGAGGTGAC TAGCTACAAT 
GGCATTCCCA GCGGTCCGCA AAAGGTGCCC ATTTGCGTGA TCAACGCGGT CACTCCCATA 
CCCACCATGT ACACGTGGGC ACCAACACAG CAGAACTTCA TGGTGGAGGA TGAGACGGTG 
CTGCACAATA TACCCTATAT GGGCGACGAG GTCCTCGATA AGGATGGCAA GTTCATAGAG 
GAACTGATCA AAAACTACGA TGGCAAGGTT CACGGCGACA AGGATCCCTC GTTCATGGAC 
GATGCCATCT TCGTAGAACT GGTGCACGCC CTTATGCGCT CCTACAGCAA GGAACTGGAG 
GAAGCCGCTC CGGGTACAGC GACTGCCATT AAAACAGAGA CGTTGGCGAA ATCAAAACAG 
GGCGAAGATG ATGGGGTAGT TGATGTTGAC GCAGATGGTG AATCTCCCAT GAAGCTTGAG 
AAAACCGACA GCAAAGGGGA TCTGACTGAA GTTGAGAAGA AGGAAACTGA AGAACCGCTG 
GAGACTGAAG ATGCGGATGT TAAGCCCGAT GTGGAAGAAG TCAAGGACAA GCTGCCCTTC 
CCTGCGCCCA TTATTTTTCA GGCCATCAGC GCTAACTTTC CAGACAAGGG CACCGCGCAA 
GAGCTCAAAG AAAAGTACAT TGAGCTTACG GAGCACCAGG ATCCGGAGCG CCCACAGGAG 
TGCACACCCA ACATTGACGG CATCAAGGCC GAGAGCGTTT CCCGGGAAAG GACTATGCAC 
TCCTTTCACA CGCTCTTCTG TCGTCGTTGC TTCAAATACG ACTGCTTCCT GCACCGCTTG 
CAAGGACATG CGGGTCCCAA TCTCCAGAAG CGTCGCTATC CGGAACTGAA GCCTTTCGCC 
GAACCTTGCA GCAACTCCTG CTACATGTTG ATCGATGGCA TGAAGGAAAA GCTGGCGGCC 
GATAGCAAGA CACCTCCCAT TGATTCCTGC AATGAGGCCA GCTCCGAGGA CAGTAATGAC 
AGCAACAGTC AGTTTAGCAA CAAGGACTTT AATCATGAGA ATAGCAAGGA TAATGGCCTG 
ACTGTAAATA GCGCCGCTGT GGCTGAGATC AACTCCATTA TGGCTGGCAT GATGAACATC 
ACCAGTACGC AGTGCGTTTG GACAGGCGCT GACCAAGCAC TTTATCGGGT CTTGCATAAA 
GTGTATTTAA AGAACTACTG CGCGATTGCG CACAACATGC TGACCAAGAC CTGTCGTCAG 
GTGTACGAGT TTGCCCAGAA GGAGGATGCT GAGTTTAGCT TCGAGGATTT GCGACAGGAC 
TTCACACCTC CGCGCAAGAA GAAGAAGAAG CAACGTCTGT GGTCGCTGCA CTGTCGTAAG 
ATTCAGCTAA AGAAGGACTC ATCCTCGAAT CACGTGTACA ACTACACGCC CTGCGACCAT 
CCCGGACATC CATGCGACAT GAACTGCTCC TGCATTCAGA CACAAAACTT CTGCGAAAAG 
TTCTGCAACT GCAGCAGCGA TTGCCAGAAT CGTTTCCCCG GCTGTCGCTG CAAGGCTCAG 
TGCAATACGA AGCAGTGTCC TTGCTATCTG GCAGTGCGCG AGTGTGATCC CGATCTGTGC 
CAGGCCTGCG GAGCGGATCA GTTTAAGCTG ACCAAGATCA CGTGCAAGAA CGTGTGCGTG 
CAGCGAGGAC TGCACAAGCA CCTGCTCATG GCTCCGTCTG ACATCGCCGG CTGGGGCATC 
TTTCTTAAGG AGGGTGCGCA AAAGAATGAG TTCATTTCGG AATATTGTGG CGAGATCATA 
TCCCAAGATG AAGCCGATCG CCGTGGCAAG GTTTACGACA AGTACATGTG CTCATTTCTT 
TTCAATCTGA ACAACGATTT TGTTGTGGAT GCCACTCGGA AGGGCAACAA GATTCGGTTT 
GCCAATCATT CCATTAATCC CAATTGCTAT GCCAAGGTTA TGATGGTCAC CGGCGATCAT 
CGCATCGGCA TCTTTGCCAA GCGGGCCATT CAGCCCGGCG AAGAGCTATT CTTTGACTAC 
AGATACGGAC CCACGGAGCA GCTGAAGTTC GTGGGCATAG AGCGTGAAAT GGAAATTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524021.2
CDS	305..2587
Translation	MNSTKVPPEWKRRVKSEYIKIRQQKRYKRADEIKEAWIRNWDEHNHNVQDLYCESKVWQAKPYDPPHVDCVKRAEVTSYNGIPSGPQKVPICVINAVTPIPTMYTWAPTQQNFMVEDETVLHNIPYMGDEVLDKDGKFIEELIKNYDGKVHGDKDPSFMDDAIFVELVHALMRSYSKELEEAAPGTATAIKTETLAKSKQGEDDGVVDVDADGESPMKLEKTDSKGDLTEVEKKETEEPLETEDADVKPDVEEVKDKLPFPAPIIFQAISANFPDKGTAQELKEKYIELTEHQDPERPQECTPNIDGIKAESVSRERTMHSFHTLFCRRCFKYDCFLHRLQGHAGPNLQKRRYPELKPFAEPCSNSCYMLIDGMKEKLAADSKTPPIDSCNEASSEDSNDSNSQFSNKDFNHENSKDNGLTVNSAAVAEINSIMAGMMNITSTQCVWTGADQALYRVLHKVYLKNYCAIAHNMLTKTCRQVYEFAQKEDAEFSFEDLRQDFTPPRKKKKKQRLWSLHCRKIQLKKDSSSNHVYNYTPCDHPGHPCDMNCSCIQTQNFCEKFCNCSSDCQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCQACGADQFKLTKITCKNVCVQRGLHKHLLMAPSDIAGWGIFLKEGAQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSINPNCYAKVMMVTGDHRIGIFAKRAIQPGEELFFDYRYGPTEQLKFVGIEREMEIV

Target ORF information:

RefSeq Version	NM_079297.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of zeste, transcript variant A (E(z)), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079297.3

ORF Insert Sequence:

ATGAATAGCA CTAAAGTGCC GCCCGAGTGG AAGAGGCGTG TCAAGTCGGA GTACATCAAA 
ATCAGGCAGC AGAAGCGCTA CAAACGTGCC GACGAGATCA AGGAGGCGTG GATAAGAAAC 
TGGGACGAGC ACAACCACAA TGTACAGGAT CTGTACTGCG AGTCGAAGGT TTGGCAGGCT 
AAACCCTACG ATCCGCCGCA TGTGGACTGT GTGAAGCGGG CGGAGGTGAC TAGCTACAAT 
GGCATTCCCA GCGGTCCGCA AAAGGTGCCC ATTTGCGTGA TCAACGCGGT CACTCCCATA 
CCCACCATGT ACACGTGGGC ACCAACACAG CAGAACTTCA TGGTGGAGGA TGAGACGGTG 
CTGCACAATA TACCCTATAT GGGCGACGAG GTCCTCGATA AGGATGGCAA GTTCATAGAG 
GAACTGATCA AAAACTACGA TGGCAAGGTT CACGGCGACA AGGATCCCTC GTTCATGGAC 
GATGCCATCT TCGTAGAACT GGTGCACGCC CTTATGCGCT CCTACAGCAA GGAACTGGAG 
GAAGCCGCTC CGGGTACAGC GACTGCCATT AAAACAGAGA CGTTGGCGAA ATCAAAACAG 
GGCGAAGATG ATGGGGTAGT TGATGTTGAC GCAGATGGTG AATCTCCCAT GAAGCTTGAG 
AAAACCGACA GCAAAGGGGA TCTGACTGAA GTTGAGAAGA AGGAAACTGA AGAACCGCTG 
GAGACTGAAG ATGCGGATGT TAAGCCCGAT GTGGAAGAAG TCAAGGACAA GCTGCCCTTC 
CCTGCGCCCA TTATTTTTCA GGCCATCAGC GCTAACTTTC CAGACAAGGG CACCGCGCAA 
GAGCTCAAAG AAAAGTACAT TGAGCTTACG GAGCACCAGG ATCCGGAGCG CCCACAGGAG 
TGCACACCCA ACATTGACGG CATCAAGGCC GAGAGCGTTT CCCGGGAAAG GACTATGCAC 
TCCTTTCACA CGCTCTTCTG TCGTCGTTGC TTCAAATACG ACTGCTTCCT GCACCGCTTG 
CAAGGACATG CGGGTCCCAA TCTCCAGAAG CGTCGCTATC CGGAACTGAA GCCTTTCGCC 
GAACCTTGCA GCAACTCCTG CTACATGTTG ATCGATGGCA TGAAGGAAAA GCTGGCGGCC 
GATAGCAAGA CACCTCCCAT TGATTCCTGC AATGAGGCCA GCTCCGAGGA CAGTAATGAC 
AGCAACAGTC AGTTTAGCAA CAAGGACTTT AATCATGAGA ATAGCAAGGA TAATGGCCTG 
ACTGTAAATA GCGCCGCTGT GGCTGAGATC AACTCCATTA TGGCTGGCAT GATGAACATC 
ACCAGTACGC AGTGCGTTTG GACAGGCGCT GACCAAGCAC TTTATCGGGT CTTGCATAAA 
GTGTATTTAA AGAACTACTG CGCGATTGCG CACAACATGC TGACCAAGAC CTGTCGTCAG 
GTGTACGAGT TTGCCCAGAA GGAGGATGCT GAGTTTAGCT TCGAGGATTT GCGACAGGAC 
TTCACACCTC CGCGCAAGAA GAAGAAGAAG CAACGTCTGT GGTCGCTGCA CTGTCGTAAG 
ATTCAGCTAA AGAAGGACTC ATCCTCGAAT CACGTGTACA ACTACACGCC CTGCGACCAT 
CCCGGACATC CATGCGACAT GAACTGCTCC TGCATTCAGA CACAAAACTT CTGCGAAAAG 
TTCTGCAACT GCAGCAGCGA TTGCCAGAAT CGTTTCCCCG GCTGTCGCTG CAAGGCTCAG 
TGCAATACGA AGCAGTGTCC TTGCTATCTG GCAGTGCGCG AGTGTGATCC CGATCTGTGC 
CAGGCCTGCG GAGCGGATCA GTTTAAGCTG ACCAAGATCA CGTGCAAGAA CGTGTGCGTG 
CAGCGAGGAC TGCACAAGCA CCTGCTCATG GCTCCGTCTG ACATCGCCGG CTGGGGCATC 
TTTCTTAAGG AGGGTGCGCA AAAGAATGAG TTCATTTCGG AATATTGTGG CGAGATCATA 
TCCCAAGATG AAGCCGATCG CCGTGGCAAG GTTTACGACA AGTACATGTG CTCATTTCTT 
TTCAATCTGA ACAACGATTT TGTTGTGGAT GCCACTCGGA AGGGCAACAA GATTCGGTTT 
GCCAATCATT CCATTAATCC CAATTGCTAT GCCAAGGTTA TGATGGTCAC CGGCGATCAT 
CGCATCGGCA TCTTTGCCAA GCGGGCCATT CAGCCCGGCG AAGAGCTATT CTTTGACTAC 
AGATACGGAC CCACGGAGCA GCTGAAGTTC GTGGGCATAG AGCGTGAAAT GGAAATTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E(z) ( NM_001274753.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261682.1 Drosophila melanogaster enhancer of zeste, transcript variant C (E(z)), mRNA.

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CloneID	OFa19891
Clone ID Related Accession (Same CDS sequence)	NM_001274753.1
Accession Version	NM_001274753.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of zeste, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATAGCA CTAAAGTGCC GCCCGAGTGG AAGAGGCGTG TCAAGTCGGA GTACATCAAA 
ATCAGGCAGC AGAAGCGCTA CAAACGTGCC GACGAGATCA AGGAGGCGTG GATAAGAAAC 
TGGGACGAGC ACAACCACAA TGTACAGGAT CTGTACTGCG AGTCGAAGGT TTGGCAGGCT 
AAACCCTACG ATCCGCCGCA TGTGGACTGT GTGAAGCGGG CGGAGGTGAC TAGCTACAAT 
GGCATTCCCA GCGGTCCGCA AAAGGTGCCC ATTTGCGTGA TCAACGCGGT CACTCCCATA 
CCCACCATGT ACACGTGGGC ACCAACACAG CAGAACTTCA TGGTGGAGGA TGAGACGGTG 
CTGCACAATA TACCCTATAT GGGCGACGAG GTCCTCGATA AGGATGGCAA GTTCATAGAG 
GAACTGATCA AAAACTACGA TGGCAAGGTT CACGGCGACA AGGATCCCTC GTTCATGGAC 
GATGCCATCT TCGTAGAACT GGTGCACGCC CTTATGCGCT CCTACAGCAA GGAACTGGAG 
GAAGCCGCTC CGGGTACAGC GACTGCCATT AAAACAGAGA CGTTGGCGAA ATCAAAACAG 
GGCGAAGATG ATGGGGTAGT TGATGTTGAC GCAGATGGTG AATCTCCCAT GAAGCTTGAG 
AAAACCGACA GCAAAGGGGA TCTGACTGAA GTTGAGAAGA AGGAAACTGA AGAACCGCTG 
GAGACTGAAG ATGCGGATGT TAAGCCCGAT GTGGAAGAAG TCAAGGACAA GCTGCCCTTC 
CCTGCGCCCA TTATTTTTCA GGCCATCAGC GCTAACTTTC CAGACAAGGG CACCGCGCAA 
GAGCTCAAAG AAAAGTACAT TGAGCTTACG GAGCACCAGG ATCCGGAGCG CCCACAGGAG 
TGCACACCCA ACATTGACGG CATCAAGGCC GAGAGCGTTT CCCGGGAAAG GACTATGCAC 
TCCTTTCACA CGCTCTTCTG TCGTCGTTGC TTCAAATACG ACTGCTTCCT GCACCGTCAC 
CATGTGCAGG GCTTGCAAGG ACATGCGGGT CCCAATCTCC AGAAGCGTCG CTATCCGGAA 
CTGAAGCCTT TCGCCGAACC TTGCAGCAAC TCCTGCTACA TGTTGATCGA TGGCATGAAG 
GAAAAGCTGG CGGCCGATAG CAAGACACCT CCCATTGATT CCTGCAATGA GGCCAGCTCC 
GAGGACAGTA ATGACAGCAA CAGTCAGTTT AGCAACAAGG ACTTTAATCA TGAGAATAGC 
AAGGATAATG GCCTGACTGT AAATAGCGCC GCTGTGGCTG AGATCAACTC CATTATGGCT 
GGCATGATGA ACATCACCAG TACGCAGTGC GTTTGGACAG GCGCTGACCA AGCACTTTAT 
CGGGTCTTGC ATAAAGTGTA TTTAAAGAAC TACTGCGCGA TTGCGCACAA CATGCTGACC 
AAGACCTGTC GTCAGGTGTA CGAGTTTGCC CAGAAGGAGG ATGCTGAGTT TAGCTTCGAG 
GATTTGCGAC AGGACTTCAC ACCTCCGCGC AAGAAGAAGA AGAAGCAACG TCTGTGGTCG 
CTGCACTGTC GTAAGATTCA GCTAAAGAAG GACTCATCCT CGAATCACGT GTACAACTAC 
ACGCCCTGCG ACCATCCCGG ACATCCATGC GACATGAACT GCTCCTGCAT TCAGACACAA 
AACTTCTGCG AAAAGTTCTG CAACTGCAGC AGCGATTGCC AGAATCGTTT CCCCGGCTGT 
CGCTGCAAGG CTCAGTGCAA TACGAAGCAG TGTCCTTGCT ATCTGGCAGT GCGCGAGTGT 
GATCCCGATC TGTGCCAGGC CTGCGGAGCG GATCAGTTTA AGCTGACCAA GATCACGTGC 
AAGAACGTGT GCGTGCAGCG AGGACTGCAC AAGCACCTGC TCATGGCTCC GTCTGACATC 
GCCGGCTGGG GCATCTTTCT TAAGGAGGGT GCGCAAAAGA ATGAGTTCAT TTCGGAATAT 
TGTGGCGAGA TCATATCCCA AGATGAAGCC GATCGCCGTG GCAAGGTTTA CGACAAGTAC 
ATGTGCTCAT TTCTTTTCAA TCTGAACAAC GATTTTGTTG TGGATGCCAC TCGGAAGGGC 
AACAAGATTC GGTTTGCCAA TCATTCCATT AATCCCAATT GCTATGCCAA GGTTATGATG 
GTCACCGGCG ATCATCGCAT CGGCATCTTT GCCAAGCGGG CCATTCAGCC CGGCGAAGAG 
CTATTCTTTG ACTACAGATA CGGACCCACG GAGCAGCTGA AGTTCGTGGG CATAGAGCGT 
GAAATGGAAA TTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261682.1
CDS	119..2416
Translation	MNSTKVPPEWKRRVKSEYIKIRQQKRYKRADEIKEAWIRNWDEHNHNVQDLYCESKVWQAKPYDPPHVDCVKRAEVTSYNGIPSGPQKVPICVINAVTPIPTMYTWAPTQQNFMVEDETVLHNIPYMGDEVLDKDGKFIEELIKNYDGKVHGDKDPSFMDDAIFVELVHALMRSYSKELEEAAPGTATAIKTETLAKSKQGEDDGVVDVDADGESPMKLEKTDSKGDLTEVEKKETEEPLETEDADVKPDVEEVKDKLPFPAPIIFQAISANFPDKGTAQELKEKYIELTEHQDPERPQECTPNIDGIKAESVSRERTMHSFHTLFCRRCFKYDCFLHRHHVQGLQGHAGPNLQKRRYPELKPFAEPCSNSCYMLIDGMKEKLAADSKTPPIDSCNEASSEDSNDSNSQFSNKDFNHENSKDNGLTVNSAAVAEINSIMAGMMNITSTQCVWTGADQALYRVLHKVYLKNYCAIAHNMLTKTCRQVYEFAQKEDAEFSFEDLRQDFTPPRKKKKKQRLWSLHCRKIQLKKDSSSNHVYNYTPCDHPGHPCDMNCSCIQTQNFCEKFCNCSSDCQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCQACGADQFKLTKITCKNVCVQRGLHKHLLMAPSDIAGWGIFLKEGAQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSINPNCYAKVMMVTGDHRIGIFAKRAIQPGEELFFDYRYGPTEQLKFVGIEREMEIV

Target ORF information:

RefSeq Version	NM_001274753.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of zeste, transcript variant C (E(z)), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274753.1

ORF Insert Sequence:

ATGAATAGCA CTAAAGTGCC GCCCGAGTGG AAGAGGCGTG TCAAGTCGGA GTACATCAAA 
ATCAGGCAGC AGAAGCGCTA CAAACGTGCC GACGAGATCA AGGAGGCGTG GATAAGAAAC 
TGGGACGAGC ACAACCACAA TGTACAGGAT CTGTACTGCG AGTCGAAGGT TTGGCAGGCT 
AAACCCTACG ATCCGCCGCA TGTGGACTGT GTGAAGCGGG CGGAGGTGAC TAGCTACAAT 
GGCATTCCCA GCGGTCCGCA AAAGGTGCCC ATTTGCGTGA TCAACGCGGT CACTCCCATA 
CCCACCATGT ACACGTGGGC ACCAACACAG CAGAACTTCA TGGTGGAGGA TGAGACGGTG 
CTGCACAATA TACCCTATAT GGGCGACGAG GTCCTCGATA AGGATGGCAA GTTCATAGAG 
GAACTGATCA AAAACTACGA TGGCAAGGTT CACGGCGACA AGGATCCCTC GTTCATGGAC 
GATGCCATCT TCGTAGAACT GGTGCACGCC CTTATGCGCT CCTACAGCAA GGAACTGGAG 
GAAGCCGCTC CGGGTACAGC GACTGCCATT AAAACAGAGA CGTTGGCGAA ATCAAAACAG 
GGCGAAGATG ATGGGGTAGT TGATGTTGAC GCAGATGGTG AATCTCCCAT GAAGCTTGAG 
AAAACCGACA GCAAAGGGGA TCTGACTGAA GTTGAGAAGA AGGAAACTGA AGAACCGCTG 
GAGACTGAAG ATGCGGATGT TAAGCCCGAT GTGGAAGAAG TCAAGGACAA GCTGCCCTTC 
CCTGCGCCCA TTATTTTTCA GGCCATCAGC GCTAACTTTC CAGACAAGGG CACCGCGCAA 
GAGCTCAAAG AAAAGTACAT TGAGCTTACG GAGCACCAGG ATCCGGAGCG CCCACAGGAG 
TGCACACCCA ACATTGACGG CATCAAGGCC GAGAGCGTTT CCCGGGAAAG GACTATGCAC 
TCCTTTCACA CGCTCTTCTG TCGTCGTTGC TTCAAATACG ACTGCTTCCT GCACCGTCAC 
CATGTGCAGG GCTTGCAAGG ACATGCGGGT CCCAATCTCC AGAAGCGTCG CTATCCGGAA 
CTGAAGCCTT TCGCCGAACC TTGCAGCAAC TCCTGCTACA TGTTGATCGA TGGCATGAAG 
GAAAAGCTGG CGGCCGATAG CAAGACACCT CCCATTGATT CCTGCAATGA GGCCAGCTCC 
GAGGACAGTA ATGACAGCAA CAGTCAGTTT AGCAACAAGG ACTTTAATCA TGAGAATAGC 
AAGGATAATG GCCTGACTGT AAATAGCGCC GCTGTGGCTG AGATCAACTC CATTATGGCT 
GGCATGATGA ACATCACCAG TACGCAGTGC GTTTGGACAG GCGCTGACCA AGCACTTTAT 
CGGGTCTTGC ATAAAGTGTA TTTAAAGAAC TACTGCGCGA TTGCGCACAA CATGCTGACC 
AAGACCTGTC GTCAGGTGTA CGAGTTTGCC CAGAAGGAGG ATGCTGAGTT TAGCTTCGAG 
GATTTGCGAC AGGACTTCAC ACCTCCGCGC AAGAAGAAGA AGAAGCAACG TCTGTGGTCG 
CTGCACTGTC GTAAGATTCA GCTAAAGAAG GACTCATCCT CGAATCACGT GTACAACTAC 
ACGCCCTGCG ACCATCCCGG ACATCCATGC GACATGAACT GCTCCTGCAT TCAGACACAA 
AACTTCTGCG AAAAGTTCTG CAACTGCAGC AGCGATTGCC AGAATCGTTT CCCCGGCTGT 
CGCTGCAAGG CTCAGTGCAA TACGAAGCAG TGTCCTTGCT ATCTGGCAGT GCGCGAGTGT 
GATCCCGATC TGTGCCAGGC CTGCGGAGCG GATCAGTTTA AGCTGACCAA GATCACGTGC 
AAGAACGTGT GCGTGCAGCG AGGACTGCAC AAGCACCTGC TCATGGCTCC GTCTGACATC 
GCCGGCTGGG GCATCTTTCT TAAGGAGGGT GCGCAAAAGA ATGAGTTCAT TTCGGAATAT 
TGTGGCGAGA TCATATCCCA AGATGAAGCC GATCGCCGTG GCAAGGTTTA CGACAAGTAC 
ATGTGCTCAT TTCTTTTCAA TCTGAACAAC GATTTTGTTG TGGATGCCAC TCGGAAGGGC 
AACAAGATTC GGTTTGCCAA TCATTCCATT AATCCCAATT GCTATGCCAA GGTTATGATG 
GTCACCGGCG ATCATCGCAT CGGCATCTTT GCCAAGCGGG CCATTCAGCC CGGCGAAGAG 
CTATTCTTTG ACTACAGATA CGGACCCACG GAGCAGCTGA AGTTCGTGGG CATAGAGCGT 
GAAATGGAAA TTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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E(z) cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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