hay cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	hay
Entrez Gene ID	39202
Full Name	haywire
Synonyms	CG8019,DhR25,DhXPB,DmXPB,Dmel\CG8019,ERCC3,Hay,TFIIH,XPB,Xpb,Xpb/hay,hwr,i50,ms(3)nc2,nc2
General protein information	Preferred Names haywire Names CG8019 gene product from transcript CG8019-RA CG8019-PA CG8019-PB hay-PA hay-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 67E5-67E5\|3-34.4 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_079296.4	NP_524020.2	haywire, isoform A
NM_001144459.2	NP_001137931.1	haywire, isoform B

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	Y66D12A.15	NP_499487.1
Eremothecium gossypii	AGOS_AFR280W	NP_985827.1
Arabidopsis thaliana (thale cress)	XPB1	NP_568592.1
Xenopus tropicalis (tropical clawed frog)	ercc3	NP_001123812.1
Canis lupus familiaris (dog)	ERCC3	XP_533314.2
Rattus norvegicus (Norway rat)	Ercc3	NP_001026814.1
Gallus gallus (chicken)	ERCC3	NP_001006523.1
Saccharomyces cerevisiae (baker's yeast)	SSL2	NP_012123.1
Arabidopsis thaliana (thale cress)	XPB2	NP_568591.1
Oryza sativa (rice)	Os01g0691600	NP_001043936.1
Pan troglodytes (chimpanzee)	ERCC3	XP_525907.3
Drosophila melanogaster (fruit fly)	hay	NP_524020.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012169	XP_320364.4
Homo sapiens (human)	ERCC3	NP_000113.1
Bos taurus (cattle)	ERCC3	NP_001039453.1
Mus musculus (house mouse)	Ercc3	NP_598419.1
Danio rerio (zebrafish)	ercc3	NP_963876.1
Kluyveromyces lactis	KLLA0E13025g	XP_454537.1
	ERCC3	XP_001086722.1
Schizosaccharomyces pombe (fission yeast)	ptr8	NP_593474.1
Magnaporthe oryzae (rice blast fungus)	MGG_06470	XP_003717094.1
Neurospora crassa	NCU06438	XP_957329.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The data demonstrate that dMYC repression and dMYC-dependent overgrowth in the Hfp hypomorph is further impaired in the C-terminal Hay/XPB mutant background.
Title: Defective Hfp-dependent transcriptional repression of dMYC is fundamental to tissue overgrowth in Drosophila XPB models.

analysis of physical and functional interactions between Drosophila homologue of Swc6/p18Hamlet subunit of the SWR1/SRCAP chromatin-remodeling complex with the DNA repair/transcription factor TFIIH
Title: Physical and functional interactions between Drosophila homologue of Swc6/p18Hamlet subunit of the SWR1/SRCAP chromatin-remodeling complex with the DNA repair/transcription factor TFIIH.

regulation of dmyc requires interaction between the transcriptional repressor Hfp and the DNA helicase subunit of TFIIH, Haywire (Hay)
Title: Hfp inhibits Drosophila myc transcription and cell growth in a TFIIH/Hay-dependent manner.

TFIIH core is initially located in the cytoplasm of syncytial blastoderm embryos, and that after mitotic division ten and until the cellular blastoderm stage, the core moves from the cytoplasm to the nucleus.
Title: TFIIH trafficking and its nuclear assembly during early Drosophila embryo development.

TFIIH is a basal transcription factor, but mutations in the subunit encoded by hay have specific effects in the transcription of some genes.
Title: RNA polymerase II 140wimp mutant and mutations in the TFIIH subunit XPB differentially affect homeotic gene expression in Drosophila.

The following hay gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hay cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18160	NM_001144459.2 Latest version!	Drosophila melanogaster haywire (hay), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa18160	NM_079296.4 Latest version!	Drosophila melanogaster haywire (hay), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hay ( NM_079296.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524020.2 Drosophila melanogaster haywire (hay), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18160
Clone ID Related Accession (Same CDS sequence)	NM_079296.4 , NM_001144459.2
Accession Version	NM_079296.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	haywire, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079296.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGACCGC CGAAAAAATC GAGAAAGGAT CGCAGCGGTG GTGACAAGTT CGGCAAAAAG 
CGGCGTGCCG AGGATGAGGC TTTCACCCAA CTGGTGGACG ACAATGATAG TTTGGATGCC 
ACAGAATCGG AAGGAATTCC GGGTGCAGCG TCCAAGAATG CGGAGACCAA TGACGAGCAA 
ATCAATACGG ATGAGTACGG CGCCAAGGAT TACCGGTCGC AGATGCAACT GCGTCCGGAT 
CACGGAAATC GACCACTTTG GGTTGCGCCC AATGGTCACG TCTTCCTGGA ATCATTCTCG 
CCCGTCTATA AGCATGCCCA CGATTTTCTT ATCGCCATTT CGGAGCCCGT CTGCCGACCC 
GAACACATTC ACGAGTACAA ACTTACCGCA TACAGTTTAT ATGCCGCCGT TTCGGTGGGA 
CTGCAAACCC ATGACATTGT GGAATACTTG AAGAGATTGA GCAAGACCAG CATTCCCGAA 
GGCATCCTTG AGTTTATACG ACTCTGCACC CTATCCTATG GCAAGGTCAA GCTGGTCTTG 
AAGCATAACA AGTACTTTAT CGAGTCACCA CATCCTGAGG TGCTGCAAAA GTTACTTAAG 
GATCCAGTGA TCCAGAAATG CCGCCTCATA CGCAGCGAGG GAGAGGATTT CATTCAGGGA 
ACCCTGGACG GCAAGGCTAT TACTCAATTC GGGACCAAAC TGCCGCCAGG AGCCACGGAC 
AAGCCGACAC CAGATCCTGC AGCAGCCGCA GGAGCTGATG GAACCACAGC AGTGCCAGAA 
GATATCACAG ACTTCTACGA GAAGATCGAC AAAGAGGAGG AGGACGAGGA TGAGGCCAAT 
CTGAAGACCG TGTCGTTTGA GGTGGCCCAG GAGAAGATCG AAGTGATTCA GAAACGATGC 
ATCGAGATAG AGCATCCTCT ATTGGCGGAG TACGATTTCC GCAACGATAC CAACAATCCA 
GACATTAATA TTGACCTCAA ACCCGCTGCC GTTCTGCGTC CATATCAGGA GAAGAGTCTG 
CGCAAGATGT TTGGCAATGG AAGAGCCCGC TCTGGTGTTA TTGTGCTTCC TTGTGGTGCA 
GGAAAATCCC TAGTGGGTGT CACAGCATGC TGCACCGTAC GAAAGAGGGC ACTAGTTCTG 
TGCAACAGTG GTGTTTCTGT GGAGCAGTGG AAGCAGCAGT TTAAGATGTG GTCCACAGCT 
GATGACAGCA TGATTTGTAG ATTCACCTCT GAAGCAAAAG ACAAACCCAT GGGCTGTGGA 
ATTCTTGTGA CCACATACTC TATGATAACG CACACGCAGA AGAGATCATG GGAAGCAGAG 
CAGACCATGC GTTGGCTGCA GGAGCAGGAA TGGGGCATCA TGGTGCTGGA CGAGGTGCAC 
ACCATCCCAG CCAAAATGTT CCGTCGCGTG CTGACCATCG TTCAATCTCA TTGCAAGCTG 
GGATTGACGG CCACACTACT GCGTGAAGAT GACAAGATTG CCGATCTCAA CTTCCTCATT 
GGACCCAAAC TGTACGAGGC CAACTGGTTA GAGCTGCAAA AGAAGGGATA TATTGCACGC 
GTGCAGTGCG CCGAGGTGTG GTGTCCCATG TCACCGGAGT TCTATCGCGA GTACCTGACC 
ACCAAGACCT CCAAAAAGAT GTTGCTCTAT GTGATGAATC CCTCCAAGTT CCGCAGCTGC 
CAGTTTCTTA TTAAATATCA CGAGCAACGA GGCGACAAAA CAATCGTCTT CTCAGATAAT 
GTGTTTGCGC TAAAACACTA TGCGATCAAG ATGAACAAGC CTTTCATCTA TGGTCCCACC 
TCGCAGAACG AACGTATCCA GATCCTCCAG AACTTTAAGT TTAACTCCAA GGTTAATACA 
ATCTTCGTGT CCAAAGTGGC AGACACCAGT TTCGATTTGC CCGAGGCTAA TGTGCTTATC 
CAGATCTCTT CGCATGGCGG CTCTCGTCGT CAGGAGGCCC AGCGTCTGGG TCGTATTCTG 
CGTGCTAAGA AGGGTGCCAT TGCCGAGGAA TACAACGCCT TCTTCTATAC ACTCGTCTCG 
CAGGATACCA TGGAGATGAG CTACTCCCGC AAGCGACAGC GGTTCTTGGT CAACCAGGGC 
TATAGCTACA AGGTTATCAC GCATCTCAAG GGCATGGACA CGGACTCGGA TTTGATGTAC 
GGCACACAGG AGGAGCAGGG ACAACTGCTG CAGTTGGTCC TATCCGCTTC CGATTTGGAC 
TGCGAGGATG AGAAGCTGCC GGGCGAGCCG GGCTACCGTC CCAGTGGCTC GGGCGGCGCC 
GTCAGACGTG TCGGCGGACT GAGCTCCATG TCTGGCGGAG ATGATGCCAT CTACTACGAA 
CATCGTAAAA AGAACATTGG CAGCGTGCAT CCGCTCTTCA AAAAATTCAG AGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524020.2
CDS	160..2556
Translation	MGPPKKSRKDRSGGDKFGKKRRAEDEAFTQLVDDNDSLDATESEGIPGAASKNAETNDEQINTDEYGAKDYRSQMQLRPDHGNRPLWVAPNGHVFLESFSPVYKHAHDFLIAISEPVCRPEHIHEYKLTAYSLYAAVSVGLQTHDIVEYLKRLSKTSIPEGILEFIRLCTLSYGKVKLVLKHNKYFIESPHPEVLQKLLKDPVIQKCRLIRSEGEDFIQGTLDGKAITQFGTKLPPGATDKPTPDPAAAAGADGTTAVPEDITDFYEKIDKEEEDEDEANLKTVSFEVAQEKIEVIQKRCIEIEHPLLAEYDFRNDTNNPDINIDLKPAAVLRPYQEKSLRKMFGNGRARSGVIVLPCGAGKSLVGVTACCTVRKRALVLCNSGVSVEQWKQQFKMWSTADDSMICRFTSEAKDKPMGCGILVTTYSMITHTQKRSWEAEQTMRWLQEQEWGIMVLDEVHTIPAKMFRRVLTIVQSHCKLGLTATLLREDDKIADLNFLIGPKLYEANWLELQKKGYIARVQCAEVWCPMSPEFYREYLTTKTSKKMLLYVMNPSKFRSCQFLIKYHEQRGDKTIVFSDNVFALKHYAIKMNKPFIYGPTSQNERIQILQNFKFNSKVNTIFVSKVADTSFDLPEANVLIQISSHGGSRRQEAQRLGRILRAKKGAIAEEYNAFFYTLVSQDTMEMSYSRKRQRFLVNQGYSYKVITHLKGMDTDSDLMYGTQEEQGQLLQLVLSASDLDCEDEKLPGEPGYRPSGSGGAVRRVGGLSSMSGGDDAIYYEHRKKNIGSVHPLFKKFRG

Target ORF information:

RefSeq Version	NM_079296.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster haywire (hay), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079296.4

ORF Insert Sequence:

ATGGGACCGC CGAAAAAATC GAGAAAGGAT CGCAGCGGTG GTGACAAGTT CGGCAAAAAG 
CGGCGTGCCG AGGATGAGGC TTTCACCCAA CTGGTGGACG ACAATGATAG TTTGGATGCC 
ACAGAATCGG AAGGAATTCC GGGTGCAGCG TCCAAGAATG CGGAGACCAA TGACGAGCAA 
ATCAATACGG ATGAGTACGG CGCCAAGGAT TACCGGTCGC AGATGCAACT GCGTCCGGAT 
CACGGAAATC GACCACTTTG GGTTGCGCCC AATGGTCACG TCTTCCTGGA ATCATTCTCG 
CCCGTCTATA AGCATGCCCA CGATTTTCTT ATCGCCATTT CGGAGCCCGT CTGCCGACCC 
GAACACATTC ACGAGTACAA ACTTACCGCA TACAGTTTAT ATGCCGCCGT TTCGGTGGGA 
CTGCAAACCC ATGACATTGT GGAATACTTG AAGAGATTGA GCAAGACCAG CATTCCCGAA 
GGCATCCTTG AGTTTATACG ACTCTGCACC CTATCCTATG GCAAGGTCAA GCTGGTCTTG 
AAGCATAACA AGTACTTTAT CGAGTCACCA CATCCTGAGG TGCTGCAAAA GTTACTTAAG 
GATCCAGTGA TCCAGAAATG CCGCCTCATA CGCAGCGAGG GAGAGGATTT CATTCAGGGA 
ACCCTGGACG GCAAGGCTAT TACTCAATTC GGGACCAAAC TGCCGCCAGG AGCCACGGAC 
AAGCCGACAC CAGATCCTGC AGCAGCCGCA GGAGCTGATG GAACCACAGC AGTGCCAGAA 
GATATCACAG ACTTCTACGA GAAGATCGAC AAAGAGGAGG AGGACGAGGA TGAGGCCAAT 
CTGAAGACCG TGTCGTTTGA GGTGGCCCAG GAGAAGATCG AAGTGATTCA GAAACGATGC 
ATCGAGATAG AGCATCCTCT ATTGGCGGAG TACGATTTCC GCAACGATAC CAACAATCCA 
GACATTAATA TTGACCTCAA ACCCGCTGCC GTTCTGCGTC CATATCAGGA GAAGAGTCTG 
CGCAAGATGT TTGGCAATGG AAGAGCCCGC TCTGGTGTTA TTGTGCTTCC TTGTGGTGCA 
GGAAAATCCC TAGTGGGTGT CACAGCATGC TGCACCGTAC GAAAGAGGGC ACTAGTTCTG 
TGCAACAGTG GTGTTTCTGT GGAGCAGTGG AAGCAGCAGT TTAAGATGTG GTCCACAGCT 
GATGACAGCA TGATTTGTAG ATTCACCTCT GAAGCAAAAG ACAAACCCAT GGGCTGTGGA 
ATTCTTGTGA CCACATACTC TATGATAACG CACACGCAGA AGAGATCATG GGAAGCAGAG 
CAGACCATGC GTTGGCTGCA GGAGCAGGAA TGGGGCATCA TGGTGCTGGA CGAGGTGCAC 
ACCATCCCAG CCAAAATGTT CCGTCGCGTG CTGACCATCG TTCAATCTCA TTGCAAGCTG 
GGATTGACGG CCACACTACT GCGTGAAGAT GACAAGATTG CCGATCTCAA CTTCCTCATT 
GGACCCAAAC TGTACGAGGC CAACTGGTTA GAGCTGCAAA AGAAGGGATA TATTGCACGC 
GTGCAGTGCG CCGAGGTGTG GTGTCCCATG TCACCGGAGT TCTATCGCGA GTACCTGACC 
ACCAAGACCT CCAAAAAGAT GTTGCTCTAT GTGATGAATC CCTCCAAGTT CCGCAGCTGC 
CAGTTTCTTA TTAAATATCA CGAGCAACGA GGCGACAAAA CAATCGTCTT CTCAGATAAT 
GTGTTTGCGC TAAAACACTA TGCGATCAAG ATGAACAAGC CTTTCATCTA TGGTCCCACC 
TCGCAGAACG AACGTATCCA GATCCTCCAG AACTTTAAGT TTAACTCCAA GGTTAATACA 
ATCTTCGTGT CCAAAGTGGC AGACACCAGT TTCGATTTGC CCGAGGCTAA TGTGCTTATC 
CAGATCTCTT CGCATGGCGG CTCTCGTCGT CAGGAGGCCC AGCGTCTGGG TCGTATTCTG 
CGTGCTAAGA AGGGTGCCAT TGCCGAGGAA TACAACGCCT TCTTCTATAC ACTCGTCTCG 
CAGGATACCA TGGAGATGAG CTACTCCCGC AAGCGACAGC GGTTCTTGGT CAACCAGGGC 
TATAGCTACA AGGTTATCAC GCATCTCAAG GGCATGGACA CGGACTCGGA TTTGATGTAC 
GGCACACAGG AGGAGCAGGG ACAACTGCTG CAGTTGGTCC TATCCGCTTC CGATTTGGAC 
TGCGAGGATG AGAAGCTGCC GGGCGAGCCG GGCTACCGTC CCAGTGGCTC GGGCGGCGCC 
GTCAGACGTG TCGGCGGACT GAGCTCCATG TCTGGCGGAG ATGATGCCAT CTACTACGAA 
CATCGTAAAA AGAACATTGG CAGCGTGCAT CCGCTCTTCA AAAAATTCAG AGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hay ( NM_001144459.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137931.1 Drosophila melanogaster haywire (hay), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18160
Clone ID Related Accession (Same CDS sequence)	NM_079296.4 , NM_001144459.2
Accession Version	NM_001144459.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	haywire, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Dec 17, 2009 this sequence version replaced NM_001144459.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGACCGC CGAAAAAATC GAGAAAGGAT CGCAGCGGTG GTGACAAGTT CGGCAAAAAG 
CGGCGTGCCG AGGATGAGGC TTTCACCCAA CTGGTGGACG ACAATGATAG TTTGGATGCC 
ACAGAATCGG AAGGAATTCC GGGTGCAGCG TCCAAGAATG CGGAGACCAA TGACGAGCAA 
ATCAATACGG ATGAGTACGG CGCCAAGGAT TACCGGTCGC AGATGCAACT GCGTCCGGAT 
CACGGAAATC GACCACTTTG GGTTGCGCCC AATGGTCACG TCTTCCTGGA ATCATTCTCG 
CCCGTCTATA AGCATGCCCA CGATTTTCTT ATCGCCATTT CGGAGCCCGT CTGCCGACCC 
GAACACATTC ACGAGTACAA ACTTACCGCA TACAGTTTAT ATGCCGCCGT TTCGGTGGGA 
CTGCAAACCC ATGACATTGT GGAATACTTG AAGAGATTGA GCAAGACCAG CATTCCCGAA 
GGCATCCTTG AGTTTATACG ACTCTGCACC CTATCCTATG GCAAGGTCAA GCTGGTCTTG 
AAGCATAACA AGTACTTTAT CGAGTCACCA CATCCTGAGG TGCTGCAAAA GTTACTTAAG 
GATCCAGTGA TCCAGAAATG CCGCCTCATA CGCAGCGAGG GAGAGGATTT CATTCAGGGA 
ACCCTGGACG GCAAGGCTAT TACTCAATTC GGGACCAAAC TGCCGCCAGG AGCCACGGAC 
AAGCCGACAC CAGATCCTGC AGCAGCCGCA GGAGCTGATG GAACCACAGC AGTGCCAGAA 
GATATCACAG ACTTCTACGA GAAGATCGAC AAAGAGGAGG AGGACGAGGA TGAGGCCAAT 
CTGAAGACCG TGTCGTTTGA GGTGGCCCAG GAGAAGATCG AAGTGATTCA GAAACGATGC 
ATCGAGATAG AGCATCCTCT ATTGGCGGAG TACGATTTCC GCAACGATAC CAACAATCCA 
GACATTAATA TTGACCTCAA ACCCGCTGCC GTTCTGCGTC CATATCAGGA GAAGAGTCTG 
CGCAAGATGT TTGGCAATGG AAGAGCCCGC TCTGGTGTTA TTGTGCTTCC TTGTGGTGCA 
GGAAAATCCC TAGTGGGTGT CACAGCATGC TGCACCGTAC GAAAGAGGGC ACTAGTTCTG 
TGCAACAGTG GTGTTTCTGT GGAGCAGTGG AAGCAGCAGT TTAAGATGTG GTCCACAGCT 
GATGACAGCA TGATTTGTAG ATTCACCTCT GAAGCAAAAG ACAAACCCAT GGGCTGTGGA 
ATTCTTGTGA CCACATACTC TATGATAACG CACACGCAGA AGAGATCATG GGAAGCAGAG 
CAGACCATGC GTTGGCTGCA GGAGCAGGAA TGGGGCATCA TGGTGCTGGA CGAGGTGCAC 
ACCATCCCAG CCAAAATGTT CCGTCGCGTG CTGACCATCG TTCAATCTCA TTGCAAGCTG 
GGATTGACGG CCACACTACT GCGTGAAGAT GACAAGATTG CCGATCTCAA CTTCCTCATT 
GGACCCAAAC TGTACGAGGC CAACTGGTTA GAGCTGCAAA AGAAGGGATA TATTGCACGC 
GTGCAGTGCG CCGAGGTGTG GTGTCCCATG TCACCGGAGT TCTATCGCGA GTACCTGACC 
ACCAAGACCT CCAAAAAGAT GTTGCTCTAT GTGATGAATC CCTCCAAGTT CCGCAGCTGC 
CAGTTTCTTA TTAAATATCA CGAGCAACGA GGCGACAAAA CAATCGTCTT CTCAGATAAT 
GTGTTTGCGC TAAAACACTA TGCGATCAAG ATGAACAAGC CTTTCATCTA TGGTCCCACC 
TCGCAGAACG AACGTATCCA GATCCTCCAG AACTTTAAGT TTAACTCCAA GGTTAATACA 
ATCTTCGTGT CCAAAGTGGC AGACACCAGT TTCGATTTGC CCGAGGCTAA TGTGCTTATC 
CAGATCTCTT CGCATGGCGG CTCTCGTCGT CAGGAGGCCC AGCGTCTGGG TCGTATTCTG 
CGTGCTAAGA AGGGTGCCAT TGCCGAGGAA TACAACGCCT TCTTCTATAC ACTCGTCTCG 
CAGGATACCA TGGAGATGAG CTACTCCCGC AAGCGACAGC GGTTCTTGGT CAACCAGGGC 
TATAGCTACA AGGTTATCAC GCATCTCAAG GGCATGGACA CGGACTCGGA TTTGATGTAC 
GGCACACAGG AGGAGCAGGG ACAACTGCTG CAGTTGGTCC TATCCGCTTC CGATTTGGAC 
TGCGAGGATG AGAAGCTGCC GGGCGAGCCG GGCTACCGTC CCAGTGGCTC GGGCGGCGCC 
GTCAGACGTG TCGGCGGACT GAGCTCCATG TCTGGCGGAG ATGATGCCAT CTACTACGAA 
CATCGTAAAA AGAACATTGG CAGCGTGCAT CCGCTCTTCA AAAAATTCAG AGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137931.1
CDS	67..2463
Translation	MGPPKKSRKDRSGGDKFGKKRRAEDEAFTQLVDDNDSLDATESEGIPGAASKNAETNDEQINTDEYGAKDYRSQMQLRPDHGNRPLWVAPNGHVFLESFSPVYKHAHDFLIAISEPVCRPEHIHEYKLTAYSLYAAVSVGLQTHDIVEYLKRLSKTSIPEGILEFIRLCTLSYGKVKLVLKHNKYFIESPHPEVLQKLLKDPVIQKCRLIRSEGEDFIQGTLDGKAITQFGTKLPPGATDKPTPDPAAAAGADGTTAVPEDITDFYEKIDKEEEDEDEANLKTVSFEVAQEKIEVIQKRCIEIEHPLLAEYDFRNDTNNPDINIDLKPAAVLRPYQEKSLRKMFGNGRARSGVIVLPCGAGKSLVGVTACCTVRKRALVLCNSGVSVEQWKQQFKMWSTADDSMICRFTSEAKDKPMGCGILVTTYSMITHTQKRSWEAEQTMRWLQEQEWGIMVLDEVHTIPAKMFRRVLTIVQSHCKLGLTATLLREDDKIADLNFLIGPKLYEANWLELQKKGYIARVQCAEVWCPMSPEFYREYLTTKTSKKMLLYVMNPSKFRSCQFLIKYHEQRGDKTIVFSDNVFALKHYAIKMNKPFIYGPTSQNERIQILQNFKFNSKVNTIFVSKVADTSFDLPEANVLIQISSHGGSRRQEAQRLGRILRAKKGAIAEEYNAFFYTLVSQDTMEMSYSRKRQRFLVNQGYSYKVITHLKGMDTDSDLMYGTQEEQGQLLQLVLSASDLDCEDEKLPGEPGYRPSGSGGAVRRVGGLSSMSGGDDAIYYEHRKKNIGSVHPLFKKFRG

Target ORF information:

RefSeq Version	NM_001144459.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster haywire (hay), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144459.2

ORF Insert Sequence:

ATGGGACCGC CGAAAAAATC GAGAAAGGAT CGCAGCGGTG GTGACAAGTT CGGCAAAAAG 
CGGCGTGCCG AGGATGAGGC TTTCACCCAA CTGGTGGACG ACAATGATAG TTTGGATGCC 
ACAGAATCGG AAGGAATTCC GGGTGCAGCG TCCAAGAATG CGGAGACCAA TGACGAGCAA 
ATCAATACGG ATGAGTACGG CGCCAAGGAT TACCGGTCGC AGATGCAACT GCGTCCGGAT 
CACGGAAATC GACCACTTTG GGTTGCGCCC AATGGTCACG TCTTCCTGGA ATCATTCTCG 
CCCGTCTATA AGCATGCCCA CGATTTTCTT ATCGCCATTT CGGAGCCCGT CTGCCGACCC 
GAACACATTC ACGAGTACAA ACTTACCGCA TACAGTTTAT ATGCCGCCGT TTCGGTGGGA 
CTGCAAACCC ATGACATTGT GGAATACTTG AAGAGATTGA GCAAGACCAG CATTCCCGAA 
GGCATCCTTG AGTTTATACG ACTCTGCACC CTATCCTATG GCAAGGTCAA GCTGGTCTTG 
AAGCATAACA AGTACTTTAT CGAGTCACCA CATCCTGAGG TGCTGCAAAA GTTACTTAAG 
GATCCAGTGA TCCAGAAATG CCGCCTCATA CGCAGCGAGG GAGAGGATTT CATTCAGGGA 
ACCCTGGACG GCAAGGCTAT TACTCAATTC GGGACCAAAC TGCCGCCAGG AGCCACGGAC 
AAGCCGACAC CAGATCCTGC AGCAGCCGCA GGAGCTGATG GAACCACAGC AGTGCCAGAA 
GATATCACAG ACTTCTACGA GAAGATCGAC AAAGAGGAGG AGGACGAGGA TGAGGCCAAT 
CTGAAGACCG TGTCGTTTGA GGTGGCCCAG GAGAAGATCG AAGTGATTCA GAAACGATGC 
ATCGAGATAG AGCATCCTCT ATTGGCGGAG TACGATTTCC GCAACGATAC CAACAATCCA 
GACATTAATA TTGACCTCAA ACCCGCTGCC GTTCTGCGTC CATATCAGGA GAAGAGTCTG 
CGCAAGATGT TTGGCAATGG AAGAGCCCGC TCTGGTGTTA TTGTGCTTCC TTGTGGTGCA 
GGAAAATCCC TAGTGGGTGT CACAGCATGC TGCACCGTAC GAAAGAGGGC ACTAGTTCTG 
TGCAACAGTG GTGTTTCTGT GGAGCAGTGG AAGCAGCAGT TTAAGATGTG GTCCACAGCT 
GATGACAGCA TGATTTGTAG ATTCACCTCT GAAGCAAAAG ACAAACCCAT GGGCTGTGGA 
ATTCTTGTGA CCACATACTC TATGATAACG CACACGCAGA AGAGATCATG GGAAGCAGAG 
CAGACCATGC GTTGGCTGCA GGAGCAGGAA TGGGGCATCA TGGTGCTGGA CGAGGTGCAC 
ACCATCCCAG CCAAAATGTT CCGTCGCGTG CTGACCATCG TTCAATCTCA TTGCAAGCTG 
GGATTGACGG CCACACTACT GCGTGAAGAT GACAAGATTG CCGATCTCAA CTTCCTCATT 
GGACCCAAAC TGTACGAGGC CAACTGGTTA GAGCTGCAAA AGAAGGGATA TATTGCACGC 
GTGCAGTGCG CCGAGGTGTG GTGTCCCATG TCACCGGAGT TCTATCGCGA GTACCTGACC 
ACCAAGACCT CCAAAAAGAT GTTGCTCTAT GTGATGAATC CCTCCAAGTT CCGCAGCTGC 
CAGTTTCTTA TTAAATATCA CGAGCAACGA GGCGACAAAA CAATCGTCTT CTCAGATAAT 
GTGTTTGCGC TAAAACACTA TGCGATCAAG ATGAACAAGC CTTTCATCTA TGGTCCCACC 
TCGCAGAACG AACGTATCCA GATCCTCCAG AACTTTAAGT TTAACTCCAA GGTTAATACA 
ATCTTCGTGT CCAAAGTGGC AGACACCAGT TTCGATTTGC CCGAGGCTAA TGTGCTTATC 
CAGATCTCTT CGCATGGCGG CTCTCGTCGT CAGGAGGCCC AGCGTCTGGG TCGTATTCTG 
CGTGCTAAGA AGGGTGCCAT TGCCGAGGAA TACAACGCCT TCTTCTATAC ACTCGTCTCG 
CAGGATACCA TGGAGATGAG CTACTCCCGC AAGCGACAGC GGTTCTTGGT CAACCAGGGC 
TATAGCTACA AGGTTATCAC GCATCTCAAG GGCATGGACA CGGACTCGGA TTTGATGTAC 
GGCACACAGG AGGAGCAGGG ACAACTGCTG CAGTTGGTCC TATCCGCTTC CGATTTGGAC 
TGCGAGGATG AGAAGCTGCC GGGCGAGCCG GGCTACCGTC CCAGTGGCTC GGGCGGCGCC 
GTCAGACGTG TCGGCGGACT GAGCTCCATG TCTGGCGGAG ATGATGCCAT CTACTACGAA 
CATCGTAAAA AGAACATTGG CAGCGTGCAT CCGCTCTTCA AAAAATTCAG AGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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hay cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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