CalpB cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CalpB
Entrez Gene ID	39165
Full Name	Calpain-B
Synonyms	CALPB,CG8107,Dmel\CG8107,calpB
General protein information	Preferred Names Calpain-B Names CG8107 gene product from transcript CG8107-RA CG8107-PA CG8107-PB CalpB-PA CalpB-PB calpain B calpainB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 67D1-67D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_079292.5	NP_524016.4	Calpain-B, isoform A
NM_001259777.2	NP_001246706.1	Calpain-B, isoform B

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Capn9	NP_076198.2
Homo sapiens (human)	CAPN9	NP_006606.1
Rattus norvegicus (Norway rat)	Capn9	NP_001258069.1
Gallus gallus (chicken)	CAPN9	XP_419585.2
Danio rerio (zebrafish)	capn9	NP_001003501.1
Pan troglodytes (chimpanzee)	CAPN9	XP_001148632.1
Drosophila melanogaster (fruit fly)	CalpB	NP_001246706.1
Drosophila melanogaster (fruit fly)	CalpA	NP_001097378.1
Canis lupus familiaris (dog)	CAPN9	XP_850025.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012068	XP_001238322.2
Xenopus tropicalis (tropical clawed frog)	capn9	NP_001121483.1
	CAPN9	XP_002802168.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study demonstrates that CalpB but not the structurally related CalpA has an important modulator function during in vivo cell migration.
Title: CalpB modulates border cell migration in Drosophila egg chambers.

Data show that mutations that disrupted endogenous calpainA or calpainB activity in transgenic flies suppressed tau toxicity.
Title: Implicating calpain in tau-mediated toxicity in vivo.

the activation of the ERK pathway by extracellular signals results in the phosphorylation and activation of calpain B in fruit flies.
Title: Regulation of calpain B from Drosophila melanogaster by phosphorylation.

CalpainB is required for AML1-ETO-induced blood cell disorders in Drosophila.
Title: A Drosophila model identifies calpains as modulators of the human leukemogenic fusion protein AML1-ETO.

initial autolysis of Calpain B seems to occur intramolecularly, which supports the monomeric nature of Drosophila calpains
Title: Drosophila Calpain B is monomeric and autolyzes intramolecularly.

The following CalpB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CalpB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18126	NM_079292.5 Latest version!	Drosophila melanogaster Calpain-B (CalpB), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa18126	NM_001259777.2 Latest version!	Drosophila melanogaster Calpain-B (CalpB), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CalpB ( NM_079292.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524016.4 Drosophila melanogaster Calpain-B (CalpB), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18126
Clone ID Related Accession (Same CDS sequence)	NM_079292.5 , NM_001259777.2
Accession Version	NM_079292.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Calpain-B, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_079292.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACGGCA TTGATAATTA CCCCAAGAAT TATCATGCCA GTGGCATTGG CCTTGTCAAT 
TTGGCGGCCT TGGGCTACAG CAAGAATGAA GTCAGCGGCG GGAACGAAGG AGGAGGAGCT 
CCCAAACCCA AAGCGGGTCT CTACCCCTCG TTGCCCTATC CATCATCCGA ATCCGTGGGC 
GGAATGCCAT ATGTGGTAAA GCAGACGAGT CATGCCCAGA ATGCTTCGTA TGCGGGACCC 
ACCATGGGCA TGGGAATGCC CGTGCCAGAA GCCCCCTCAG CGCCGGCTCC ATACCCCAGT 
GCCACGCCAT ACCCTGGATC AGGACTTTAT CCTAGTCTGC CATCAGCCAA CGTATCATCG 
CTGCCGTACC CCACTGCGCC TATGGCGCCC TATCCAACCG GAATGCCCTA TCCAACTGGC 
ATGCCGCAGC CCAATCTACC CTATCCGGCG GCTCCGCTCG CACCATATCC ATCTGCCATG 
CCCGGTTTGC CCGGTATGCC AATGCCGTAC GCTCCCATGC CAACGAGTCC GGCTCCCCAG 
CACAATATCG GATTCCCAGC ACTACCATAT CCCACGGCAC CGCCACCGGA AAGTGCTCCA 
ACCCAGGAGG AGGAACCTTC CGTCGGCGTG GCCGAGCTAA GCTTCACCAG CGTGAAGGTG 
CCCGAGAACC AGAACATGTT TTGGATGGGT CGCAAGGCCA CTAGTGCCCG GCAGAACAGC 
GTAAGCAAAG GCGATTTCCA ATCGCTGCGC GACAGTTGCC TGGCTAATGG AACCATGTTC 
GAGGATCCCG ATTTTCCGGC CACCAACGCT TCGCTGATGT ACTCACGACG ACCGGATCGC 
TACTACGAGT GGCTGCGTCC CGGTGACATC GCCGATGATC CGCAGTTCTT CGTGGAAGGC 
TATTCACGAT TCGATGTGCA ACAAGGAGAA CTTGGCGATT GCTGGCTACT GGCTGCAGCT 
GCCAATTTGA CGCAGGACTC GACGCTCTTC TTTAGGGTAA TCCCGCCGGA TCAGGACTTC 
CAGGAGAACT ACGCTGGCAT CTTTCACTTC AAGTTTTGGC AGTATGGCAA GTGGGTGGAA 
GTGGTCATTG ATGACCGTCT GCCCACCTAT AATGGCGAGC TAATCTACAT GCACTCCACG 
GAGAAGAACG AGTTTTGGAG TGCCTTGCTG GAGAAGGCTT ATGCAAAGCT GCATGGATCT 
TATGAAGCAC TCAAGGGCGG CACCACGTGC GAGGCCATGG AGGACTTTAC TGGCGGCGTC 
ACCGAGTGGT ACGACATCAA GGAGGCGCCC CCAAATCTAT TCAGCATCAT GATGAAGGCC 
GCCGAGCGTG GCTCGATGAT GGGCTGCTCT TTGGAGCCGG ATCCACATGT TCTGGAAGCG 
GAGACGCCAC AAGGTTTGAT TCGCGGTCAT GCATACTCCA TTACCAAGGT GTGCCTCATG 
GACATATCGA CGCCCAATCG GCAGGGAAAA TTGCCCATGA TACGTATGCG TAATCCATGG 
GGCAACGATG CCGAATGGAG CGGTCCGTGG AGCGACAGCT CGCCTGAGTG GCGATTTATT 
CCGGAGCACA CGAAGGAGGA GATTGGCCTG AATTTCGATA GGGACGGCGA GTTCTGGATG 
TCCTTCCAGG ACTTCCTCAA TCATTTTGAT CGCGTGGAGA TATGTAATTT GTCGCCCGAC 
TCGCTGACTG AGGATCAGCA GCACAGCTCT CGCCGCAAGT GGGAAATGTC CATGTTCGAG 
GGTGAGTGGA CATCGGGAGT AACAGCCGGC GGTTGCAGAA ACTTCCTGGA AACCTTCTGG 
CACAATCCGC AGTACATCAT TTCGCTGGAG GATCCCGACG ACGAGGATGA CGATGGCAAA 
TGCACGGCTA TTGTGGCCCT TATGCAGAAG AATCGACGTA GCAAGCGCAA CGTGGGCATC 
GACTGTCTGA CCATCGGATT TGCTATATAT CATCTCACAG ACCGTGACAT GCAAGTGAAG 
CCCCAGGGAC TCAACTTCTT CAAGTACCGA GCATCGGTGG CGCGTTCACC ACATTTTATC 
AACACGCGCG AGGTTTGCGC CCGGTTTAAG TTGCCACCTG GCCACTACCT GATTGTTCCA 
TCGACCTTCG ATCCGAATGA GGAGGGCGAG TTCATTATTC GCGTCTTCTC GGAGACAAGG 
AACAATATGG AGGAGAACGA CGATGAGGTT GGCTTTGGCG AGACCGACGA TCGCATTGCT 
CCGTCTCTGC CGCCACCGAC TCCAAAGGAG GAGGATGATC CGCAGCGCAT TGCACTGCGT 
CGTCTGTTCG ACAGCGTGGC TGGCAGCGAT GAGGAAGTCG ACTGGCAGGA GCTGAAGCGC 
ATCCTGGACC ACTCTATGCG CGATGTTATG GTGGGCAGTG ATGGCTTCTC CAAGGATGCC 
GTGCGTTCGA TGGTAGCCAT GCTGGACAAG GATCGCTCCG GTCGCCTTGG TTTCGAGGAG 
TTCGAAGCTC TGCTTACCGA TATTGCCAAG TGGCGGGCGG TCTTTAAGCT ATATGACACC 
CGTCGCACTG GCAGTATTGA TGGATTCCAT CTGCGCGGAG CACTCAATTC GGCTGGCTAT 
CATCTGAACA ACCGGCTCCT GAACGCCCTG GCCCATCGCT ACGGATCGCG GGAGGGACAA 
ATACCATTCG ACGATTTCCT GATGTGTGCC ATCAAGGTTA GAACATTCAT CGAGATGTTC 
CGCGAACGGG ACACGGACAA TTCGGACACG GCCTTCTTCA ACTTGGACGA CTGGCTGGAG 
CGAACAATTT ACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524016.4
CDS	523..3300
Translation	MYGIDNYPKNYHASGIGLVNLAALGYSKNEVSGGNEGGGAPKPKAGLYPSLPYPSSESVGGMPYVVKQTSHAQNASYAGPTMGMGMPVPEAPSAPAPYPSATPYPGSGLYPSLPSANVSSLPYPTAPMAPYPTGMPYPTGMPQPNLPYPAAPLAPYPSAMPGLPGMPMPYAPMPTSPAPQHNIGFPALPYPTAPPPESAPTQEEEPSVGVAELSFTSVKVPENQNMFWMGRKATSARQNSVSKGDFQSLRDSCLANGTMFEDPDFPATNASLMYSRRPDRYYEWLRPGDIADDPQFFVEGYSRFDVQQGELGDCWLLAAAANLTQDSTLFFRVIPPDQDFQENYAGIFHFKFWQYGKWVEVVIDDRLPTYNGELIYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGTTCEAMEDFTGGVTEWYDIKEAPPNLFSIMMKAAERGSMMGCSLEPDPHVLEAETPQGLIRGHAYSITKVCLMDISTPNRQGKLPMIRMRNPWGNDAEWSGPWSDSSPEWRFIPEHTKEEIGLNFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQHSSRRKWEMSMFEGEWTSGVTAGGCRNFLETFWHNPQYIISLEDPDDEDDDGKCTAIVALMQKNRRSKRNVGIDCLTIGFAIYHLTDRDMQVKPQGLNFFKYRASVARSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETRNNMEENDDEVGFGETDDRIAPSLPPPTPKEEDDPQRIALRRLFDSVAGSDEEVDWQELKRILDHSMRDVMVGSDGFSKDAVRSMVAMLDKDRSGRLGFEEFEALLTDIAKWRAVFKLYDTRRTGSIDGFHLRGALNSAGYHLNNRLLNALAHRYGSREGQIPFDDFLMCAIKVRTFIEMFRERDTDNSDTAFFNLDDWLERTIYS

Target ORF information:

RefSeq Version	NM_079292.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Calpain-B (CalpB), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079292.5

ORF Insert Sequence:

ATGTACGGCA TTGATAATTA CCCCAAGAAT TATCATGCCA GTGGCATTGG CCTTGTCAAT 
TTGGCGGCCT TGGGCTACAG CAAGAATGAA GTCAGCGGCG GGAACGAAGG AGGAGGAGCT 
CCCAAACCCA AAGCGGGTCT CTACCCCTCG TTGCCCTATC CATCATCCGA ATCCGTGGGC 
GGAATGCCAT ATGTGGTAAA GCAGACGAGT CATGCCCAGA ATGCTTCGTA TGCGGGACCC 
ACCATGGGCA TGGGAATGCC CGTGCCAGAA GCCCCCTCAG CGCCGGCTCC ATACCCCAGT 
GCCACGCCAT ACCCTGGATC AGGACTTTAT CCTAGTCTGC CATCAGCCAA CGTATCATCG 
CTGCCGTACC CCACTGCGCC TATGGCGCCC TATCCAACCG GAATGCCCTA TCCAACTGGC 
ATGCCGCAGC CCAATCTACC CTATCCGGCG GCTCCGCTCG CACCATATCC ATCTGCCATG 
CCCGGTTTGC CCGGTATGCC AATGCCGTAC GCTCCCATGC CAACGAGTCC GGCTCCCCAG 
CACAATATCG GATTCCCAGC ACTACCATAT CCCACGGCAC CGCCACCGGA AAGTGCTCCA 
ACCCAGGAGG AGGAACCTTC CGTCGGCGTG GCCGAGCTAA GCTTCACCAG CGTGAAGGTG 
CCCGAGAACC AGAACATGTT TTGGATGGGT CGCAAGGCCA CTAGTGCCCG GCAGAACAGC 
GTAAGCAAAG GCGATTTCCA ATCGCTGCGC GACAGTTGCC TGGCTAATGG AACCATGTTC 
GAGGATCCCG ATTTTCCGGC CACCAACGCT TCGCTGATGT ACTCACGACG ACCGGATCGC 
TACTACGAGT GGCTGCGTCC CGGTGACATC GCCGATGATC CGCAGTTCTT CGTGGAAGGC 
TATTCACGAT TCGATGTGCA ACAAGGAGAA CTTGGCGATT GCTGGCTACT GGCTGCAGCT 
GCCAATTTGA CGCAGGACTC GACGCTCTTC TTTAGGGTAA TCCCGCCGGA TCAGGACTTC 
CAGGAGAACT ACGCTGGCAT CTTTCACTTC AAGTTTTGGC AGTATGGCAA GTGGGTGGAA 
GTGGTCATTG ATGACCGTCT GCCCACCTAT AATGGCGAGC TAATCTACAT GCACTCCACG 
GAGAAGAACG AGTTTTGGAG TGCCTTGCTG GAGAAGGCTT ATGCAAAGCT GCATGGATCT 
TATGAAGCAC TCAAGGGCGG CACCACGTGC GAGGCCATGG AGGACTTTAC TGGCGGCGTC 
ACCGAGTGGT ACGACATCAA GGAGGCGCCC CCAAATCTAT TCAGCATCAT GATGAAGGCC 
GCCGAGCGTG GCTCGATGAT GGGCTGCTCT TTGGAGCCGG ATCCACATGT TCTGGAAGCG 
GAGACGCCAC AAGGTTTGAT TCGCGGTCAT GCATACTCCA TTACCAAGGT GTGCCTCATG 
GACATATCGA CGCCCAATCG GCAGGGAAAA TTGCCCATGA TACGTATGCG TAATCCATGG 
GGCAACGATG CCGAATGGAG CGGTCCGTGG AGCGACAGCT CGCCTGAGTG GCGATTTATT 
CCGGAGCACA CGAAGGAGGA GATTGGCCTG AATTTCGATA GGGACGGCGA GTTCTGGATG 
TCCTTCCAGG ACTTCCTCAA TCATTTTGAT CGCGTGGAGA TATGTAATTT GTCGCCCGAC 
TCGCTGACTG AGGATCAGCA GCACAGCTCT CGCCGCAAGT GGGAAATGTC CATGTTCGAG 
GGTGAGTGGA CATCGGGAGT AACAGCCGGC GGTTGCAGAA ACTTCCTGGA AACCTTCTGG 
CACAATCCGC AGTACATCAT TTCGCTGGAG GATCCCGACG ACGAGGATGA CGATGGCAAA 
TGCACGGCTA TTGTGGCCCT TATGCAGAAG AATCGACGTA GCAAGCGCAA CGTGGGCATC 
GACTGTCTGA CCATCGGATT TGCTATATAT CATCTCACAG ACCGTGACAT GCAAGTGAAG 
CCCCAGGGAC TCAACTTCTT CAAGTACCGA GCATCGGTGG CGCGTTCACC ACATTTTATC 
AACACGCGCG AGGTTTGCGC CCGGTTTAAG TTGCCACCTG GCCACTACCT GATTGTTCCA 
TCGACCTTCG ATCCGAATGA GGAGGGCGAG TTCATTATTC GCGTCTTCTC GGAGACAAGG 
AACAATATGG AGGAGAACGA CGATGAGGTT GGCTTTGGCG AGACCGACGA TCGCATTGCT 
CCGTCTCTGC CGCCACCGAC TCCAAAGGAG GAGGATGATC CGCAGCGCAT TGCACTGCGT 
CGTCTGTTCG ACAGCGTGGC TGGCAGCGAT GAGGAAGTCG ACTGGCAGGA GCTGAAGCGC 
ATCCTGGACC ACTCTATGCG CGATGTTATG GTGGGCAGTG ATGGCTTCTC CAAGGATGCC 
GTGCGTTCGA TGGTAGCCAT GCTGGACAAG GATCGCTCCG GTCGCCTTGG TTTCGAGGAG 
TTCGAAGCTC TGCTTACCGA TATTGCCAAG TGGCGGGCGG TCTTTAAGCT ATATGACACC 
CGTCGCACTG GCAGTATTGA TGGATTCCAT CTGCGCGGAG CACTCAATTC GGCTGGCTAT 
CATCTGAACA ACCGGCTCCT GAACGCCCTG GCCCATCGCT ACGGATCGCG GGAGGGACAA 
ATACCATTCG ACGATTTCCT GATGTGTGCC ATCAAGGTTA GAACATTCAT CGAGATGTTC 
CGCGAACGGG ACACGGACAA TTCGGACACG GCCTTCTTCA ACTTGGACGA CTGGCTGGAG 
CGAACAATTT ACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CalpB ( NM_001259777.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246706.1 Drosophila melanogaster Calpain-B (CalpB), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18126
Clone ID Related Accession (Same CDS sequence)	NM_079292.5 , NM_001259777.2
Accession Version	NM_001259777.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Calpain-B, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001259777.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACGGCA TTGATAATTA CCCCAAGAAT TATCATGCCA GTGGCATTGG CCTTGTCAAT 
TTGGCGGCCT TGGGCTACAG CAAGAATGAA GTCAGCGGCG GGAACGAAGG AGGAGGAGCT 
CCCAAACCCA AAGCGGGTCT CTACCCCTCG TTGCCCTATC CATCATCCGA ATCCGTGGGC 
GGAATGCCAT ATGTGGTAAA GCAGACGAGT CATGCCCAGA ATGCTTCGTA TGCGGGACCC 
ACCATGGGCA TGGGAATGCC CGTGCCAGAA GCCCCCTCAG CGCCGGCTCC ATACCCCAGT 
GCCACGCCAT ACCCTGGATC AGGACTTTAT CCTAGTCTGC CATCAGCCAA CGTATCATCG 
CTGCCGTACC CCACTGCGCC TATGGCGCCC TATCCAACCG GAATGCCCTA TCCAACTGGC 
ATGCCGCAGC CCAATCTACC CTATCCGGCG GCTCCGCTCG CACCATATCC ATCTGCCATG 
CCCGGTTTGC CCGGTATGCC AATGCCGTAC GCTCCCATGC CAACGAGTCC GGCTCCCCAG 
CACAATATCG GATTCCCAGC ACTACCATAT CCCACGGCAC CGCCACCGGA AAGTGCTCCA 
ACCCAGGAGG AGGAACCTTC CGTCGGCGTG GCCGAGCTAA GCTTCACCAG CGTGAAGGTG 
CCCGAGAACC AGAACATGTT TTGGATGGGT CGCAAGGCCA CTAGTGCCCG GCAGAACAGC 
GTAAGCAAAG GCGATTTCCA ATCGCTGCGC GACAGTTGCC TGGCTAATGG AACCATGTTC 
GAGGATCCCG ATTTTCCGGC CACCAACGCT TCGCTGATGT ACTCACGACG ACCGGATCGC 
TACTACGAGT GGCTGCGTCC CGGTGACATC GCCGATGATC CGCAGTTCTT CGTGGAAGGC 
TATTCACGAT TCGATGTGCA ACAAGGAGAA CTTGGCGATT GCTGGCTACT GGCTGCAGCT 
GCCAATTTGA CGCAGGACTC GACGCTCTTC TTTAGGGTAA TCCCGCCGGA TCAGGACTTC 
CAGGAGAACT ACGCTGGCAT CTTTCACTTC AAGTTTTGGC AGTATGGCAA GTGGGTGGAA 
GTGGTCATTG ATGACCGTCT GCCCACCTAT AATGGCGAGC TAATCTACAT GCACTCCACG 
GAGAAGAACG AGTTTTGGAG TGCCTTGCTG GAGAAGGCTT ATGCAAAGCT GCATGGATCT 
TATGAAGCAC TCAAGGGCGG CACCACGTGC GAGGCCATGG AGGACTTTAC TGGCGGCGTC 
ACCGAGTGGT ACGACATCAA GGAGGCGCCC CCAAATCTAT TCAGCATCAT GATGAAGGCC 
GCCGAGCGTG GCTCGATGAT GGGCTGCTCT TTGGAGCCGG ATCCACATGT TCTGGAAGCG 
GAGACGCCAC AAGGTTTGAT TCGCGGTCAT GCATACTCCA TTACCAAGGT GTGCCTCATG 
GACATATCGA CGCCCAATCG GCAGGGAAAA TTGCCCATGA TACGTATGCG TAATCCATGG 
GGCAACGATG CCGAATGGAG CGGTCCGTGG AGCGACAGCT CGCCTGAGTG GCGATTTATT 
CCGGAGCACA CGAAGGAGGA GATTGGCCTG AATTTCGATA GGGACGGCGA GTTCTGGATG 
TCCTTCCAGG ACTTCCTCAA TCATTTTGAT CGCGTGGAGA TATGTAATTT GTCGCCCGAC 
TCGCTGACTG AGGATCAGCA GCACAGCTCT CGCCGCAAGT GGGAAATGTC CATGTTCGAG 
GGTGAGTGGA CATCGGGAGT AACAGCCGGC GGTTGCAGAA ACTTCCTGGA AACCTTCTGG 
CACAATCCGC AGTACATCAT TTCGCTGGAG GATCCCGACG ACGAGGATGA CGATGGCAAA 
TGCACGGCTA TTGTGGCCCT TATGCAGAAG AATCGACGTA GCAAGCGCAA CGTGGGCATC 
GACTGTCTGA CCATCGGATT TGCTATATAT CATCTCACAG ACCGTGACAT GCAAGTGAAG 
CCCCAGGGAC TCAACTTCTT CAAGTACCGA GCATCGGTGG CGCGTTCACC ACATTTTATC 
AACACGCGCG AGGTTTGCGC CCGGTTTAAG TTGCCACCTG GCCACTACCT GATTGTTCCA 
TCGACCTTCG ATCCGAATGA GGAGGGCGAG TTCATTATTC GCGTCTTCTC GGAGACAAGG 
AACAATATGG AGGAGAACGA CGATGAGGTT GGCTTTGGCG AGACCGACGA TCGCATTGCT 
CCGTCTCTGC CGCCACCGAC TCCAAAGGAG GAGGATGATC CGCAGCGCAT TGCACTGCGT 
CGTCTGTTCG ACAGCGTGGC TGGCAGCGAT GAGGAAGTCG ACTGGCAGGA GCTGAAGCGC 
ATCCTGGACC ACTCTATGCG CGATGTTATG GTGGGCAGTG ATGGCTTCTC CAAGGATGCC 
GTGCGTTCGA TGGTAGCCAT GCTGGACAAG GATCGCTCCG GTCGCCTTGG TTTCGAGGAG 
TTCGAAGCTC TGCTTACCGA TATTGCCAAG TGGCGGGCGG TCTTTAAGCT ATATGACACC 
CGTCGCACTG GCAGTATTGA TGGATTCCAT CTGCGCGGAG CACTCAATTC GGCTGGCTAT 
CATCTGAACA ACCGGCTCCT GAACGCCCTG GCCCATCGCT ACGGATCGCG GGAGGGACAA 
ATACCATTCG ACGATTTCCT GATGTGTGCC ATCAAGGTTA GAACATTCAT CGAGATGTTC 
CGCGAACGGG ACACGGACAA TTCGGACACG GCCTTCTTCA ACTTGGACGA CTGGCTGGAG 
CGAACAATTT ACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246706.1
CDS	181..2958
Translation	MYGIDNYPKNYHASGIGLVNLAALGYSKNEVSGGNEGGGAPKPKAGLYPSLPYPSSESVGGMPYVVKQTSHAQNASYAGPTMGMGMPVPEAPSAPAPYPSATPYPGSGLYPSLPSANVSSLPYPTAPMAPYPTGMPYPTGMPQPNLPYPAAPLAPYPSAMPGLPGMPMPYAPMPTSPAPQHNIGFPALPYPTAPPPESAPTQEEEPSVGVAELSFTSVKVPENQNMFWMGRKATSARQNSVSKGDFQSLRDSCLANGTMFEDPDFPATNASLMYSRRPDRYYEWLRPGDIADDPQFFVEGYSRFDVQQGELGDCWLLAAAANLTQDSTLFFRVIPPDQDFQENYAGIFHFKFWQYGKWVEVVIDDRLPTYNGELIYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGTTCEAMEDFTGGVTEWYDIKEAPPNLFSIMMKAAERGSMMGCSLEPDPHVLEAETPQGLIRGHAYSITKVCLMDISTPNRQGKLPMIRMRNPWGNDAEWSGPWSDSSPEWRFIPEHTKEEIGLNFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQHSSRRKWEMSMFEGEWTSGVTAGGCRNFLETFWHNPQYIISLEDPDDEDDDGKCTAIVALMQKNRRSKRNVGIDCLTIGFAIYHLTDRDMQVKPQGLNFFKYRASVARSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETRNNMEENDDEVGFGETDDRIAPSLPPPTPKEEDDPQRIALRRLFDSVAGSDEEVDWQELKRILDHSMRDVMVGSDGFSKDAVRSMVAMLDKDRSGRLGFEEFEALLTDIAKWRAVFKLYDTRRTGSIDGFHLRGALNSAGYHLNNRLLNALAHRYGSREGQIPFDDFLMCAIKVRTFIEMFRERDTDNSDTAFFNLDDWLERTIYS

Target ORF information:

RefSeq Version	NM_001259777.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Calpain-B (CalpB), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259777.2

ORF Insert Sequence:

ATGTACGGCA TTGATAATTA CCCCAAGAAT TATCATGCCA GTGGCATTGG CCTTGTCAAT 
TTGGCGGCCT TGGGCTACAG CAAGAATGAA GTCAGCGGCG GGAACGAAGG AGGAGGAGCT 
CCCAAACCCA AAGCGGGTCT CTACCCCTCG TTGCCCTATC CATCATCCGA ATCCGTGGGC 
GGAATGCCAT ATGTGGTAAA GCAGACGAGT CATGCCCAGA ATGCTTCGTA TGCGGGACCC 
ACCATGGGCA TGGGAATGCC CGTGCCAGAA GCCCCCTCAG CGCCGGCTCC ATACCCCAGT 
GCCACGCCAT ACCCTGGATC AGGACTTTAT CCTAGTCTGC CATCAGCCAA CGTATCATCG 
CTGCCGTACC CCACTGCGCC TATGGCGCCC TATCCAACCG GAATGCCCTA TCCAACTGGC 
ATGCCGCAGC CCAATCTACC CTATCCGGCG GCTCCGCTCG CACCATATCC ATCTGCCATG 
CCCGGTTTGC CCGGTATGCC AATGCCGTAC GCTCCCATGC CAACGAGTCC GGCTCCCCAG 
CACAATATCG GATTCCCAGC ACTACCATAT CCCACGGCAC CGCCACCGGA AAGTGCTCCA 
ACCCAGGAGG AGGAACCTTC CGTCGGCGTG GCCGAGCTAA GCTTCACCAG CGTGAAGGTG 
CCCGAGAACC AGAACATGTT TTGGATGGGT CGCAAGGCCA CTAGTGCCCG GCAGAACAGC 
GTAAGCAAAG GCGATTTCCA ATCGCTGCGC GACAGTTGCC TGGCTAATGG AACCATGTTC 
GAGGATCCCG ATTTTCCGGC CACCAACGCT TCGCTGATGT ACTCACGACG ACCGGATCGC 
TACTACGAGT GGCTGCGTCC CGGTGACATC GCCGATGATC CGCAGTTCTT CGTGGAAGGC 
TATTCACGAT TCGATGTGCA ACAAGGAGAA CTTGGCGATT GCTGGCTACT GGCTGCAGCT 
GCCAATTTGA CGCAGGACTC GACGCTCTTC TTTAGGGTAA TCCCGCCGGA TCAGGACTTC 
CAGGAGAACT ACGCTGGCAT CTTTCACTTC AAGTTTTGGC AGTATGGCAA GTGGGTGGAA 
GTGGTCATTG ATGACCGTCT GCCCACCTAT AATGGCGAGC TAATCTACAT GCACTCCACG 
GAGAAGAACG AGTTTTGGAG TGCCTTGCTG GAGAAGGCTT ATGCAAAGCT GCATGGATCT 
TATGAAGCAC TCAAGGGCGG CACCACGTGC GAGGCCATGG AGGACTTTAC TGGCGGCGTC 
ACCGAGTGGT ACGACATCAA GGAGGCGCCC CCAAATCTAT TCAGCATCAT GATGAAGGCC 
GCCGAGCGTG GCTCGATGAT GGGCTGCTCT TTGGAGCCGG ATCCACATGT TCTGGAAGCG 
GAGACGCCAC AAGGTTTGAT TCGCGGTCAT GCATACTCCA TTACCAAGGT GTGCCTCATG 
GACATATCGA CGCCCAATCG GCAGGGAAAA TTGCCCATGA TACGTATGCG TAATCCATGG 
GGCAACGATG CCGAATGGAG CGGTCCGTGG AGCGACAGCT CGCCTGAGTG GCGATTTATT 
CCGGAGCACA CGAAGGAGGA GATTGGCCTG AATTTCGATA GGGACGGCGA GTTCTGGATG 
TCCTTCCAGG ACTTCCTCAA TCATTTTGAT CGCGTGGAGA TATGTAATTT GTCGCCCGAC 
TCGCTGACTG AGGATCAGCA GCACAGCTCT CGCCGCAAGT GGGAAATGTC CATGTTCGAG 
GGTGAGTGGA CATCGGGAGT AACAGCCGGC GGTTGCAGAA ACTTCCTGGA AACCTTCTGG 
CACAATCCGC AGTACATCAT TTCGCTGGAG GATCCCGACG ACGAGGATGA CGATGGCAAA 
TGCACGGCTA TTGTGGCCCT TATGCAGAAG AATCGACGTA GCAAGCGCAA CGTGGGCATC 
GACTGTCTGA CCATCGGATT TGCTATATAT CATCTCACAG ACCGTGACAT GCAAGTGAAG 
CCCCAGGGAC TCAACTTCTT CAAGTACCGA GCATCGGTGG CGCGTTCACC ACATTTTATC 
AACACGCGCG AGGTTTGCGC CCGGTTTAAG TTGCCACCTG GCCACTACCT GATTGTTCCA 
TCGACCTTCG ATCCGAATGA GGAGGGCGAG TTCATTATTC GCGTCTTCTC GGAGACAAGG 
AACAATATGG AGGAGAACGA CGATGAGGTT GGCTTTGGCG AGACCGACGA TCGCATTGCT 
CCGTCTCTGC CGCCACCGAC TCCAAAGGAG GAGGATGATC CGCAGCGCAT TGCACTGCGT 
CGTCTGTTCG ACAGCGTGGC TGGCAGCGAT GAGGAAGTCG ACTGGCAGGA GCTGAAGCGC 
ATCCTGGACC ACTCTATGCG CGATGTTATG GTGGGCAGTG ATGGCTTCTC CAAGGATGCC 
GTGCGTTCGA TGGTAGCCAT GCTGGACAAG GATCGCTCCG GTCGCCTTGG TTTCGAGGAG 
TTCGAAGCTC TGCTTACCGA TATTGCCAAG TGGCGGGCGG TCTTTAAGCT ATATGACACC 
CGTCGCACTG GCAGTATTGA TGGATTCCAT CTGCGCGGAG CACTCAATTC GGCTGGCTAT 
CATCTGAACA ACCGGCTCCT GAACGCCCTG GCCCATCGCT ACGGATCGCG GGAGGGACAA 
ATACCATTCG ACGATTTCCT GATGTGTGCC ATCAAGGTTA GAACATTCAT CGAGATGTTC 
CGCGAACGGG ACACGGACAA TTCGGACACG GCCTTCTTCA ACTTGGACGA CTGGCTGGAG 
CGAACAATTT ACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CalpB cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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