iPLA2-VIA cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	iPLA2-VIA
Entrez Gene ID	39160
Full Name	calcium-independent phospholipase A2 VIA
Synonyms	CG6718,Dmel\CG6718
General protein information	Preferred Names calcium-independent phospholipase A2 VIA Names CG6718 gene product from transcript CG6718-RA CG6718-PA CG6718-PB CG6718-PC CG6718-PD iPLA2-VIA-PA iPLA2-VIA-PB iPLA2-VIA-PC iPLA2-VIA-PD phospholipase A[[2]]
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 67C11-67C11

mRNA and Protein(s)

mRNA	Protein	Name
NM_168384.2	NP_729566.2	calcium-independent phospholipase A2 VIA, isoform C
NM_140109.2	NP_648366.2	calcium-independent phospholipase A2 VIA, isoform A
NM_168383.2	NP_729565.2	calcium-independent phospholipase A2 VIA, isoform B
NM_168385.2	NP_729567.2	calcium-independent phospholipase A2 VIA, isoform D

Pathways from BioSystems

Homologies

	PLA2G6	XP_002798434.1
Caenorhabditis elegans (roundworm)	F47A4.5	NP_509647.1
Pan troglodytes (chimpanzee)	PLA2G6	XP_003317276.1
Canis lupus familiaris (dog)	PLA2G6	XP_861095.2
Gallus gallus (chicken)	PLA2G6	NP_001124210.1
Mus musculus (house mouse)	Pla2g6	NP_001185952.1
Xenopus tropicalis (tropical clawed frog)	pla2g6	NP_001072661.1
Bos taurus (cattle)	PLA2G6	XP_002687969.2
Danio rerio (zebrafish)	pla2g6	NP_998262.1
Drosophila melanogaster (fruit fly)	iPLA2-VIA	NP_648366.2
Caenorhabditis elegans (roundworm)	CELE_W07A8.2	NP_508000.1
Homo sapiens (human)	PLA2G6	NP_003551.2
Rattus norvegicus (Norway rat)	Pla2g6	NP_001005560.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004812	XP_317997.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our findings demonstrate that loss of normal PLA2G6 gene activity leads to lipid peroxidation, mitochondrial dysfunction and subsequent mitochondrial membrane abnormalities.
Title: Loss of PLA2G6 leads to elevated mitochondrial lipid peroxidation and mitochondrial dysfunction.

The following iPLA2-VIA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the iPLA2-VIA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18122	NM_140109.2 Latest version!	Drosophila melanogaster calcium-independent phospholipase A2 VIA (iPLA2-VIA), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa18123	NM_168384.2 Latest version!	Drosophila melanogaster calcium-independent phospholipase A2 VIA (iPLA2-VIA), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa18123	NM_168383.2 Latest version!	Drosophila melanogaster calcium-independent phospholipase A2 VIA (iPLA2-VIA), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa18123	NM_168385.2 Latest version!	Drosophila melanogaster calcium-independent phospholipase A2 VIA (iPLA2-VIA), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

iPLA2-VIA ( NM_140109.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648366.2 Drosophila melanogaster calcium-independent phospholipase A2 VIA (iPLA2-VIA), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18122
Clone ID Related Accession (Same CDS sequence)	NM_140109.2
Accession Version	NM_140109.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	calcium-independent phospholipase A2 VIA, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_140109.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCAATG TTCTACAGCG CCTGCTGGGT GTCGATCAGC CGGCCAATAA GGTCGTGGAA 
ATTAAGAGCG AAGCGCTGGG GACACTTCAA GTCCTGGCCC GCGATGACGC CATGCTCCTC 
TTTGCACCGC CCTTCAACAG CAGCAATGAA AAACGAGCCG TCTATGAGAT CATTTTGCAG 
CGACCCACCT CGGATTCCAA TACCACATCC TTCAGTCTGT ACCGGTCGCC GGTGCAGCAG 
GAAGCCGAGG AACGCTTCAA TGCCTTTCTG CAAAGGCTGC CCGTTTTCGT CAGCATTGTG 
AAAGAGTACT ACAATGTAAA TGGGCTGCAG AAAGCTTGCG ATGCCTTGGC TGATAATCCC 
TCCTGGACGC TGTCCCATTT GATTGCCTAC TTCAATCTGG TGGACTACAT TAGCAATCCC 
AAGATGCTCC AGTGCGTGGA CCAAGCAGAT GCCGCCACCC TAATGTCGCC CTTCCAGTTG 
GCCATCAAAC AGGGTCACAT GGAGATGGTG AAGGCTCTGC TGCCGCTGAG CAAGCTGGAA 
CACTTGGATA TAAACAGCAA TTCGGTGTTT CATTACGCCG CCAGCACCAC CAAAGAGATT 
ATCAATCTTA TCATCGATAA AAGCACGGTA AATCTGAATC ATCTCAACTC CGATGGGTAC 
ACACCACTTC ATGTCGCCTG CCTCGCCGAC AAGCCAGAGA ATGTGAAGGC ACTGTTGCTG 
GCTGGGGCCA ATGTCAATCT CAATGCCAAG GACATCCGCA AAGTGTACAA GACATCCGCA 
CCGACCACGG TTTCCTCGTT TCTGCGCACC AATGTTAGCA AGCTGTACAC ACAGGACATG 
AAATATGGCG GCACTCCTCT GCACTGGTGT TCCTCCCGTG AAACCCTGCA TGCGCTGATC 
ATGGAGGGTT GTGATGTCAA CGCCACGAAC TTTGATGGAC GGACAGCACT GCACGTGATG 
GTGGCCAGGA ATCGGTTCGA GTGCGTTGTC ACACTACTGG CCCACGATGC GGAGATCGAT 
GTGCTGGACA AAGACGGAAA TGCAGCTTTG CACATTGCCA TTGAGAAGAA ACTAGTGCCG 
ATCGTCCAAT GTCTGGTGGT CTTTGGGTGT GACATCAATC TGAAGAACAA GGATGGCAAA 
ACGCCACGTC ACATGGTTGG CAATGATGCT AGTGGCAATA AGGATGATGA AATCCTATAC 
ATTCTGCACT CTGTGGGGGC TAAGCGCTGC AAGGATACTG GTTCAAAGTG CCCGCCTGGT 
TGCAATGCCA AAGGTAACTA CAACGGCATT CCGCCAGAGG CACCGGAATC CGTGGAGCAG 
CGCGAGCATA TAGAGCACAT GCTGGCCACC ACCAGTCGAC AAATGATGGG TGGTTTTTTG 
AACGCAGCGG CCAATGGAAT ATTGGAGAAG CAACAACCGG CACAAAAACC AGTCGTGGTC 
GATACGGAGA AAGAATTAAA AGGCCAAAGT ATAATGGATG CCCTGCTTGG CATGTTTACA 
ACCAAAGTGA ATGCGGACGA GATGAAGAAG GAGAACTCGT CGGATAGTTT GGCCAGTGGC 
TCGCAAAAAT CCGCTGTGTC TAGTCCGGAG CAGCTGCCTT CACCCACCTC GCCCATTGCC 
GCTGAAATAG GCGATAAACC CTACGGACGC GGACGTCTGC TCTGCTTGGA TGGTGGTGGC 
ATTCGTGGTC TAGTCCTGGT ACAGATGCTA CTCGAAATAG AAAAGCTTTC TCGCACTCCT 
ATTATCCATA TGTTCGACTG GATTGCCGGC ACCAGTACTG GCGGAATTCT AGCTTTGGCG 
CTGGGTTGCG GCAAAACGAT GCGTCAATGC ATGGGCCTCT ATTTGCGCAT GAAGGAGCAG 
TGTTTTGTGG GCTCACGGCC CTATAACAGC GAGTTTTTCG AGTCCATTTT GAAGGACAAT 
CTGGGCGAGT TTAACGTGAT GACGGATATT AAGCACCCGA AGATCATGGT CACTGGTGTG 
ATGGCGGATC GCAAGCCGGT CGATCTACAT CTATTCCGCA ACTATACGAG CGCCAGTGAC 
ATTTTGGGCA TTGTGACTCC AATTAACAAC CGAAGAATTC CTCCTCCTCA GCCTTCGGAA 
CAATTGGTAT GGCGTGCTGC TCGGGCTACT GGAGCTGCGC CCTCATATTT CCGCGCATTT 
GGTCGCTTTC TGGACGGCGG CTTGATTGCC AACAACCCGA CTCTAGACGC CATGACCGAG 
ATTCACGAGT ACAATATGGC CTTGCGTAGC GCTGGACGCG AATCAGAGGC CATACCTGTA 
TCCGTGGTGA TGTCGCTGGG CACGGGTCAT ATTCCGGTGA CTGAACTTAA GGACATTGAT 
GTCTTCCGGC CGGAGAGCAT CTGGGATACG GCCAAACTGG CCTACGGAAT CTCTACCATT 
GGCAATCTGC TAGTGGACCA GGCCACCTGC TCCGATGGAA GAGTAGTGGA CAGAGCTCGC 
GCCTGGTGCA GCACGATTGG CATACCCTAC TTCAGGTTCA ATCCTCAGCT GAGCGAGGAC 
ATCGCCATGG ACGAAAAGGA CGACCAAAAA CTGATCAATA TGCTGTGGCA CACTAAGGCG 
TATATGCACG CCAATCGGAA TAAGATAATC GAGATGATCA ATTTCCTAAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648366.2
CDS	148..2781
Translation	MFNVLQRLLGVDQPANKVVEIKSEALGTLQVLARDDAMLLFAPPFNSSNEKRAVYEIILQRPTSDSNTTSFSLYRSPVQQEAEERFNAFLQRLPVFVSIVKEYYNVNGLQKACDALADNPSWTLSHLIAYFNLVDYISNPKMLQCVDQADAATLMSPFQLAIKQGHMEMVKALLPLSKLEHLDINSNSVFHYAASTTKEIINLIIDKSTVNLNHLNSDGYTPLHVACLADKPENVKALLLAGANVNLNAKDIRKVYKTSAPTTVSSFLRTNVSKLYTQDMKYGGTPLHWCSSRETLHALIMEGCDVNATNFDGRTALHVMVARNRFECVVTLLAHDAEIDVLDKDGNAALHIAIEKKLVPIVQCLVVFGCDINLKNKDGKTPRHMVGNDASGNKDDEILYILHSVGAKRCKDTGSKCPPGCNAKGNYNGIPPEAPESVEQREHIEHMLATTSRQMMGGFLNAAANGILEKQQPAQKPVVVDTEKELKGQSIMDALLGMFTTKVNADEMKKENSSDSLASGSQKSAVSSPEQLPSPTSPIAAEIGDKPYGRGRLLCLDGGGIRGLVLVQMLLEIEKLSRTPIIHMFDWIAGTSTGGILALALGCGKTMRQCMGLYLRMKEQCFVGSRPYNSEFFESILKDNLGEFNVMTDIKHPKIMVTGVMADRKPVDLHLFRNYTSASDILGIVTPINNRRIPPPQPSEQLVWRAARATGAAPSYFRAFGRFLDGGLIANNPTLDAMTEIHEYNMALRSAGRESEAIPVSVVMSLGTGHIPVTELKDIDVFRPESIWDTAKLAYGISTIGNLLVDQATCSDGRVVDRARAWCSTIGIPYFRFNPQLSEDIAMDEKDDQKLINMLWHTKAYMHANRNKIIEMINFLK

Target ORF information:

RefSeq Version	NM_140109.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster calcium-independent phospholipase A2 VIA (iPLA2-VIA), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140109.2

ORF Insert Sequence:

ATGTTCAATG TTCTACAGCG CCTGCTGGGT GTCGATCAGC CGGCCAATAA GGTCGTGGAA 
ATTAAGAGCG AAGCGCTGGG GACACTTCAA GTCCTGGCCC GCGATGACGC CATGCTCCTC 
TTTGCACCGC CCTTCAACAG CAGCAATGAA AAACGAGCCG TCTATGAGAT CATTTTGCAG 
CGACCCACCT CGGATTCCAA TACCACATCC TTCAGTCTGT ACCGGTCGCC GGTGCAGCAG 
GAAGCCGAGG AACGCTTCAA TGCCTTTCTG CAAAGGCTGC CCGTTTTCGT CAGCATTGTG 
AAAGAGTACT ACAATGTAAA TGGGCTGCAG AAAGCTTGCG ATGCCTTGGC TGATAATCCC 
TCCTGGACGC TGTCCCATTT GATTGCCTAC TTCAATCTGG TGGACTACAT TAGCAATCCC 
AAGATGCTCC AGTGCGTGGA CCAAGCAGAT GCCGCCACCC TAATGTCGCC CTTCCAGTTG 
GCCATCAAAC AGGGTCACAT GGAGATGGTG AAGGCTCTGC TGCCGCTGAG CAAGCTGGAA 
CACTTGGATA TAAACAGCAA TTCGGTGTTT CATTACGCCG CCAGCACCAC CAAAGAGATT 
ATCAATCTTA TCATCGATAA AAGCACGGTA AATCTGAATC ATCTCAACTC CGATGGGTAC 
ACACCACTTC ATGTCGCCTG CCTCGCCGAC AAGCCAGAGA ATGTGAAGGC ACTGTTGCTG 
GCTGGGGCCA ATGTCAATCT CAATGCCAAG GACATCCGCA AAGTGTACAA GACATCCGCA 
CCGACCACGG TTTCCTCGTT TCTGCGCACC AATGTTAGCA AGCTGTACAC ACAGGACATG 
AAATATGGCG GCACTCCTCT GCACTGGTGT TCCTCCCGTG AAACCCTGCA TGCGCTGATC 
ATGGAGGGTT GTGATGTCAA CGCCACGAAC TTTGATGGAC GGACAGCACT GCACGTGATG 
GTGGCCAGGA ATCGGTTCGA GTGCGTTGTC ACACTACTGG CCCACGATGC GGAGATCGAT 
GTGCTGGACA AAGACGGAAA TGCAGCTTTG CACATTGCCA TTGAGAAGAA ACTAGTGCCG 
ATCGTCCAAT GTCTGGTGGT CTTTGGGTGT GACATCAATC TGAAGAACAA GGATGGCAAA 
ACGCCACGTC ACATGGTTGG CAATGATGCT AGTGGCAATA AGGATGATGA AATCCTATAC 
ATTCTGCACT CTGTGGGGGC TAAGCGCTGC AAGGATACTG GTTCAAAGTG CCCGCCTGGT 
TGCAATGCCA AAGGTAACTA CAACGGCATT CCGCCAGAGG CACCGGAATC CGTGGAGCAG 
CGCGAGCATA TAGAGCACAT GCTGGCCACC ACCAGTCGAC AAATGATGGG TGGTTTTTTG 
AACGCAGCGG CCAATGGAAT ATTGGAGAAG CAACAACCGG CACAAAAACC AGTCGTGGTC 
GATACGGAGA AAGAATTAAA AGGCCAAAGT ATAATGGATG CCCTGCTTGG CATGTTTACA 
ACCAAAGTGA ATGCGGACGA GATGAAGAAG GAGAACTCGT CGGATAGTTT GGCCAGTGGC 
TCGCAAAAAT CCGCTGTGTC TAGTCCGGAG CAGCTGCCTT CACCCACCTC GCCCATTGCC 
GCTGAAATAG GCGATAAACC CTACGGACGC GGACGTCTGC TCTGCTTGGA TGGTGGTGGC 
ATTCGTGGTC TAGTCCTGGT ACAGATGCTA CTCGAAATAG AAAAGCTTTC TCGCACTCCT 
ATTATCCATA TGTTCGACTG GATTGCCGGC ACCAGTACTG GCGGAATTCT AGCTTTGGCG 
CTGGGTTGCG GCAAAACGAT GCGTCAATGC ATGGGCCTCT ATTTGCGCAT GAAGGAGCAG 
TGTTTTGTGG GCTCACGGCC CTATAACAGC GAGTTTTTCG AGTCCATTTT GAAGGACAAT 
CTGGGCGAGT TTAACGTGAT GACGGATATT AAGCACCCGA AGATCATGGT CACTGGTGTG 
ATGGCGGATC GCAAGCCGGT CGATCTACAT CTATTCCGCA ACTATACGAG CGCCAGTGAC 
ATTTTGGGCA TTGTGACTCC AATTAACAAC CGAAGAATTC CTCCTCCTCA GCCTTCGGAA 
CAATTGGTAT GGCGTGCTGC TCGGGCTACT GGAGCTGCGC CCTCATATTT CCGCGCATTT 
GGTCGCTTTC TGGACGGCGG CTTGATTGCC AACAACCCGA CTCTAGACGC CATGACCGAG 
ATTCACGAGT ACAATATGGC CTTGCGTAGC GCTGGACGCG AATCAGAGGC CATACCTGTA 
TCCGTGGTGA TGTCGCTGGG CACGGGTCAT ATTCCGGTGA CTGAACTTAA GGACATTGAT 
GTCTTCCGGC CGGAGAGCAT CTGGGATACG GCCAAACTGG CCTACGGAAT CTCTACCATT 
GGCAATCTGC TAGTGGACCA GGCCACCTGC TCCGATGGAA GAGTAGTGGA CAGAGCTCGC 
GCCTGGTGCA GCACGATTGG CATACCCTAC TTCAGGTTCA ATCCTCAGCT GAGCGAGGAC 
ATCGCCATGG ACGAAAAGGA CGACCAAAAA CTGATCAATA TGCTGTGGCA CACTAAGGCG 
TATATGCACG CCAATCGGAA TAAGATAATC GAGATGATCA ATTTCCTAAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

iPLA2-VIA ( NM_168384.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729566.2 Drosophila melanogaster calcium-independent phospholipase A2 VIA (iPLA2-VIA), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa18123
Clone ID Related Accession (Same CDS sequence)	NM_168384.2 , NM_168383.2 , NM_168385.2
Accession Version	NM_168384.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	calcium-independent phospholipase A2 VIA, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_168384.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGTGGA TGGCGTTAGG GGCACTAGCC TCCGGTTTCG TTCTACAGCG CCTGCTGGGT 
GTCGATCAGC CGGCCAATAA GGTCGTGGAA ATTAAGAGCG AAGCGCTGGG GACACTTCAA 
GTCCTGGCCC GCGATGACGC CATGCTCCTC TTTGCACCGC CCTTCAACAG CAGCAATGAA 
AAACGAGCCG TCTATGAGAT CATTTTGCAG CGACCCACCT CGGATTCCAA TACCACATCC 
TTCAGTCTGT ACCGGTCGCC GGTGCAGCAG GAAGCCGAGG AACGCTTCAA TGCCTTTCTG 
CAAAGGCTGC CCGTTTTCGT CAGCATTGTG AAAGAGTACT ACAATGTAAA TGGGCTGCAG 
AAAGCTTGCG ATGCCTTGGC TGATAATCCC TCCTGGACGC TGTCCCATTT GATTGCCTAC 
TTCAATCTGG TGGACTACAT TAGCAATCCC AAGATGCTCC AGTGCGTGGA CCAAGCAGAT 
GCCGCCACCC TAATGTCGCC CTTCCAGTTG GCCATCAAAC AGGGTCACAT GGAGATGGTG 
AAGGCTCTGC TGCCGCTGAG CAAGCTGGAA CACTTGGATA TAAACAGCAA TTCGGTGTTT 
CATTACGCCG CCAGCACCAC CAAAGAGATT ATCAATCTTA TCATCGATAA AAGCACGGTA 
AATCTGAATC ATCTCAACTC CGATGGGTAC ACACCACTTC ATGTCGCCTG CCTCGCCGAC 
AAGCCAGAGA ATGTGAAGGC ACTGTTGCTG GCTGGGGCCA ATGTCAATCT CAATGCCAAG 
GACATCCGCA AAGTGTACAA GACATCCGCA CCGACCACGG TTTCCTCGTT TCTGCGCACC 
AATGTTAGCA AGCTGTACAC ACAGGACATG AAATATGGCG GCACTCCTCT GCACTGGTGT 
TCCTCCCGTG AAACCCTGCA TGCGCTGATC ATGGAGGGTT GTGATGTCAA CGCCACGAAC 
TTTGATGGAC GGACAGCACT GCACGTGATG GTGGCCAGGA ATCGGTTCGA GTGCGTTGTC 
ACACTACTGG CCCACGATGC GGAGATCGAT GTGCTGGACA AAGACGGAAA TGCAGCTTTG 
CACATTGCCA TTGAGAAGAA ACTAGTGCCG ATCGTCCAAT GTCTGGTGGT CTTTGGGTGT 
GACATCAATC TGAAGAACAA GGATGGCAAA ACGCCACGTC ACATGGTTGG CAATGATGCT 
AGTGGCAATA AGGATGATGA AATCCTATAC ATTCTGCACT CTGTGGGGGC TAAGCGCTGC 
AAGGATACTG GTTCAAAGTG CCCGCCTGGT TGCAATGCCA AAGGTAACTA CAACGGCATT 
CCGCCAGAGG CACCGGAATC CGTGGAGCAG CGCGAGCATA TAGAGCACAT GCTGGCCACC 
ACCAGTCGAC AAATGATGGG TGGTTTTTTG AACGCAGCGG CCAATGGAAT ATTGGAGAAG 
CAACAACCGG CACAAAAACC AGTCGTGGTC GATACGGAGA AAGAATTAAA AGGCCAAAGT 
ATAATGGATG CCCTGCTTGG CATGTTTACA ACCAAAGTGA ATGCGGACGA GATGAAGAAG 
GAGAACTCGT CGGATAGTTT GGCCAGTGGC TCGCAAAAAT CCGCTGTGTC TAGTCCGGAG 
CAGCTGCCTT CACCCACCTC GCCCATTGCC GCTGAAATAG GCGATAAACC CTACGGACGC 
GGACGTCTGC TCTGCTTGGA TGGTGGTGGC ATTCGTGGTC TAGTCCTGGT ACAGATGCTA 
CTCGAAATAG AAAAGCTTTC TCGCACTCCT ATTATCCATA TGTTCGACTG GATTGCCGGC 
ACCAGTACTG GCGGAATTCT AGCTTTGGCG CTGGGTTGCG GCAAAACGAT GCGTCAATGC 
ATGGGCCTCT ATTTGCGCAT GAAGGAGCAG TGTTTTGTGG GCTCACGGCC CTATAACAGC 
GAGTTTTTCG AGTCCATTTT GAAGGACAAT CTGGGCGAGT TTAACGTGAT GACGGATATT 
AAGCACCCGA AGATCATGGT CACTGGTGTG ATGGCGGATC GCAAGCCGGT CGATCTACAT 
CTATTCCGCA ACTATACGAG CGCCAGTGAC ATTTTGGGCA TTGTGACTCC AATTAACAAC 
CGAAGAATTC CTCCTCCTCA GCCTTCGGAA CAATTGGTAT GGCGTGCTGC TCGGGCTACT 
GGAGCTGCGC CCTCATATTT CCGCGCATTT GGTCGCTTTC TGGACGGCGG CTTGATTGCC 
AACAACCCGA CTCTAGACGC CATGACCGAG ATTCACGAGT ACAATATGGC CTTGCGTAGC 
GCTGGACGCG AATCAGAGGC CATACCTGTA TCCGTGGTGA TGTCGCTGGG CACGGGTCAT 
ATTCCGGTGA CTGAACTTAA GGACATTGAT GTCTTCCGGC CGGAGAGCAT CTGGGATACG 
GCCAAACTGG CCTACGGAAT CTCTACCATT GGCAATCTGC TAGTGGACCA GGCCACCTGC 
TCCGATGGAA GAGTAGTGGA CAGAGCTCGC GCCTGGTGCA GCACGATTGG CATACCCTAC 
TTCAGGTTCA ATCCTCAGCT GAGCGAGGAC ATCGCCATGG ACGAAAAGGA CGACCAAAAA 
CTGATCAATA TGCTGTGGCA CACTAAGGCG TATATGCACG CCAATCGGAA TAAGATAATC 
GAGATGATCA ATTTCCTAAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729566.2
CDS	534..3197
Translation	MAWMALGALASGFVLQRLLGVDQPANKVVEIKSEALGTLQVLARDDAMLLFAPPFNSSNEKRAVYEIILQRPTSDSNTTSFSLYRSPVQQEAEERFNAFLQRLPVFVSIVKEYYNVNGLQKACDALADNPSWTLSHLIAYFNLVDYISNPKMLQCVDQADAATLMSPFQLAIKQGHMEMVKALLPLSKLEHLDINSNSVFHYAASTTKEIINLIIDKSTVNLNHLNSDGYTPLHVACLADKPENVKALLLAGANVNLNAKDIRKVYKTSAPTTVSSFLRTNVSKLYTQDMKYGGTPLHWCSSRETLHALIMEGCDVNATNFDGRTALHVMVARNRFECVVTLLAHDAEIDVLDKDGNAALHIAIEKKLVPIVQCLVVFGCDINLKNKDGKTPRHMVGNDASGNKDDEILYILHSVGAKRCKDTGSKCPPGCNAKGNYNGIPPEAPESVEQREHIEHMLATTSRQMMGGFLNAAANGILEKQQPAQKPVVVDTEKELKGQSIMDALLGMFTTKVNADEMKKENSSDSLASGSQKSAVSSPEQLPSPTSPIAAEIGDKPYGRGRLLCLDGGGIRGLVLVQMLLEIEKLSRTPIIHMFDWIAGTSTGGILALALGCGKTMRQCMGLYLRMKEQCFVGSRPYNSEFFESILKDNLGEFNVMTDIKHPKIMVTGVMADRKPVDLHLFRNYTSASDILGIVTPINNRRIPPPQPSEQLVWRAARATGAAPSYFRAFGRFLDGGLIANNPTLDAMTEIHEYNMALRSAGRESEAIPVSVVMSLGTGHIPVTELKDIDVFRPESIWDTAKLAYGISTIGNLLVDQATCSDGRVVDRARAWCSTIGIPYFRFNPQLSEDIAMDEKDDQKLINMLWHTKAYMHANRNKIIEMINFLK

Target ORF information:

RefSeq Version	NM_168384.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster calcium-independent phospholipase A2 VIA (iPLA2-VIA), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168384.2

ORF Insert Sequence:

ATGGCGTGGA TGGCGTTAGG GGCACTAGCC TCCGGTTTCG TTCTACAGCG CCTGCTGGGT 
GTCGATCAGC CGGCCAATAA GGTCGTGGAA ATTAAGAGCG AAGCGCTGGG GACACTTCAA 
GTCCTGGCCC GCGATGACGC CATGCTCCTC TTTGCACCGC CCTTCAACAG CAGCAATGAA 
AAACGAGCCG TCTATGAGAT CATTTTGCAG CGACCCACCT CGGATTCCAA TACCACATCC 
TTCAGTCTGT ACCGGTCGCC GGTGCAGCAG GAAGCCGAGG AACGCTTCAA TGCCTTTCTG 
CAAAGGCTGC CCGTTTTCGT CAGCATTGTG AAAGAGTACT ACAATGTAAA TGGGCTGCAG 
AAAGCTTGCG ATGCCTTGGC TGATAATCCC TCCTGGACGC TGTCCCATTT GATTGCCTAC 
TTCAATCTGG TGGACTACAT TAGCAATCCC AAGATGCTCC AGTGCGTGGA CCAAGCAGAT 
GCCGCCACCC TAATGTCGCC CTTCCAGTTG GCCATCAAAC AGGGTCACAT GGAGATGGTG 
AAGGCTCTGC TGCCGCTGAG CAAGCTGGAA CACTTGGATA TAAACAGCAA TTCGGTGTTT 
CATTACGCCG CCAGCACCAC CAAAGAGATT ATCAATCTTA TCATCGATAA AAGCACGGTA 
AATCTGAATC ATCTCAACTC CGATGGGTAC ACACCACTTC ATGTCGCCTG CCTCGCCGAC 
AAGCCAGAGA ATGTGAAGGC ACTGTTGCTG GCTGGGGCCA ATGTCAATCT CAATGCCAAG 
GACATCCGCA AAGTGTACAA GACATCCGCA CCGACCACGG TTTCCTCGTT TCTGCGCACC 
AATGTTAGCA AGCTGTACAC ACAGGACATG AAATATGGCG GCACTCCTCT GCACTGGTGT 
TCCTCCCGTG AAACCCTGCA TGCGCTGATC ATGGAGGGTT GTGATGTCAA CGCCACGAAC 
TTTGATGGAC GGACAGCACT GCACGTGATG GTGGCCAGGA ATCGGTTCGA GTGCGTTGTC 
ACACTACTGG CCCACGATGC GGAGATCGAT GTGCTGGACA AAGACGGAAA TGCAGCTTTG 
CACATTGCCA TTGAGAAGAA ACTAGTGCCG ATCGTCCAAT GTCTGGTGGT CTTTGGGTGT 
GACATCAATC TGAAGAACAA GGATGGCAAA ACGCCACGTC ACATGGTTGG CAATGATGCT 
AGTGGCAATA AGGATGATGA AATCCTATAC ATTCTGCACT CTGTGGGGGC TAAGCGCTGC 
AAGGATACTG GTTCAAAGTG CCCGCCTGGT TGCAATGCCA AAGGTAACTA CAACGGCATT 
CCGCCAGAGG CACCGGAATC CGTGGAGCAG CGCGAGCATA TAGAGCACAT GCTGGCCACC 
ACCAGTCGAC AAATGATGGG TGGTTTTTTG AACGCAGCGG CCAATGGAAT ATTGGAGAAG 
CAACAACCGG CACAAAAACC AGTCGTGGTC GATACGGAGA AAGAATTAAA AGGCCAAAGT 
ATAATGGATG CCCTGCTTGG CATGTTTACA ACCAAAGTGA ATGCGGACGA GATGAAGAAG 
GAGAACTCGT CGGATAGTTT GGCCAGTGGC TCGCAAAAAT CCGCTGTGTC TAGTCCGGAG 
CAGCTGCCTT CACCCACCTC GCCCATTGCC GCTGAAATAG GCGATAAACC CTACGGACGC 
GGACGTCTGC TCTGCTTGGA TGGTGGTGGC ATTCGTGGTC TAGTCCTGGT ACAGATGCTA 
CTCGAAATAG AAAAGCTTTC TCGCACTCCT ATTATCCATA TGTTCGACTG GATTGCCGGC 
ACCAGTACTG GCGGAATTCT AGCTTTGGCG CTGGGTTGCG GCAAAACGAT GCGTCAATGC 
ATGGGCCTCT ATTTGCGCAT GAAGGAGCAG TGTTTTGTGG GCTCACGGCC CTATAACAGC 
GAGTTTTTCG AGTCCATTTT GAAGGACAAT CTGGGCGAGT TTAACGTGAT GACGGATATT 
AAGCACCCGA AGATCATGGT CACTGGTGTG ATGGCGGATC GCAAGCCGGT CGATCTACAT 
CTATTCCGCA ACTATACGAG CGCCAGTGAC ATTTTGGGCA TTGTGACTCC AATTAACAAC 
CGAAGAATTC CTCCTCCTCA GCCTTCGGAA CAATTGGTAT GGCGTGCTGC TCGGGCTACT 
GGAGCTGCGC CCTCATATTT CCGCGCATTT GGTCGCTTTC TGGACGGCGG CTTGATTGCC 
AACAACCCGA CTCTAGACGC CATGACCGAG ATTCACGAGT ACAATATGGC CTTGCGTAGC 
GCTGGACGCG AATCAGAGGC CATACCTGTA TCCGTGGTGA TGTCGCTGGG CACGGGTCAT 
ATTCCGGTGA CTGAACTTAA GGACATTGAT GTCTTCCGGC CGGAGAGCAT CTGGGATACG 
GCCAAACTGG CCTACGGAAT CTCTACCATT GGCAATCTGC TAGTGGACCA GGCCACCTGC 
TCCGATGGAA GAGTAGTGGA CAGAGCTCGC GCCTGGTGCA GCACGATTGG CATACCCTAC 
TTCAGGTTCA ATCCTCAGCT GAGCGAGGAC ATCGCCATGG ACGAAAAGGA CGACCAAAAA 
CTGATCAATA TGCTGTGGCA CACTAAGGCG TATATGCACG CCAATCGGAA TAAGATAATC 
GAGATGATCA ATTTCCTAAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

iPLA2-VIA ( NM_168383.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729565.2 Drosophila melanogaster calcium-independent phospholipase A2 VIA (iPLA2-VIA), transcript variant B, mRNA.

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CloneID	OFa18123
Clone ID Related Accession (Same CDS sequence)	NM_168384.2 , NM_168383.2 , NM_168385.2
Accession Version	NM_168383.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	calcium-independent phospholipase A2 VIA, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_168383.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGTGGA TGGCGTTAGG GGCACTAGCC TCCGGTTTCG TTCTACAGCG CCTGCTGGGT 
GTCGATCAGC CGGCCAATAA GGTCGTGGAA ATTAAGAGCG AAGCGCTGGG GACACTTCAA 
GTCCTGGCCC GCGATGACGC CATGCTCCTC TTTGCACCGC CCTTCAACAG CAGCAATGAA 
AAACGAGCCG TCTATGAGAT CATTTTGCAG CGACCCACCT CGGATTCCAA TACCACATCC 
TTCAGTCTGT ACCGGTCGCC GGTGCAGCAG GAAGCCGAGG AACGCTTCAA TGCCTTTCTG 
CAAAGGCTGC CCGTTTTCGT CAGCATTGTG AAAGAGTACT ACAATGTAAA TGGGCTGCAG 
AAAGCTTGCG ATGCCTTGGC TGATAATCCC TCCTGGACGC TGTCCCATTT GATTGCCTAC 
TTCAATCTGG TGGACTACAT TAGCAATCCC AAGATGCTCC AGTGCGTGGA CCAAGCAGAT 
GCCGCCACCC TAATGTCGCC CTTCCAGTTG GCCATCAAAC AGGGTCACAT GGAGATGGTG 
AAGGCTCTGC TGCCGCTGAG CAAGCTGGAA CACTTGGATA TAAACAGCAA TTCGGTGTTT 
CATTACGCCG CCAGCACCAC CAAAGAGATT ATCAATCTTA TCATCGATAA AAGCACGGTA 
AATCTGAATC ATCTCAACTC CGATGGGTAC ACACCACTTC ATGTCGCCTG CCTCGCCGAC 
AAGCCAGAGA ATGTGAAGGC ACTGTTGCTG GCTGGGGCCA ATGTCAATCT CAATGCCAAG 
GACATCCGCA AAGTGTACAA GACATCCGCA CCGACCACGG TTTCCTCGTT TCTGCGCACC 
AATGTTAGCA AGCTGTACAC ACAGGACATG AAATATGGCG GCACTCCTCT GCACTGGTGT 
TCCTCCCGTG AAACCCTGCA TGCGCTGATC ATGGAGGGTT GTGATGTCAA CGCCACGAAC 
TTTGATGGAC GGACAGCACT GCACGTGATG GTGGCCAGGA ATCGGTTCGA GTGCGTTGTC 
ACACTACTGG CCCACGATGC GGAGATCGAT GTGCTGGACA AAGACGGAAA TGCAGCTTTG 
CACATTGCCA TTGAGAAGAA ACTAGTGCCG ATCGTCCAAT GTCTGGTGGT CTTTGGGTGT 
GACATCAATC TGAAGAACAA GGATGGCAAA ACGCCACGTC ACATGGTTGG CAATGATGCT 
AGTGGCAATA AGGATGATGA AATCCTATAC ATTCTGCACT CTGTGGGGGC TAAGCGCTGC 
AAGGATACTG GTTCAAAGTG CCCGCCTGGT TGCAATGCCA AAGGTAACTA CAACGGCATT 
CCGCCAGAGG CACCGGAATC CGTGGAGCAG CGCGAGCATA TAGAGCACAT GCTGGCCACC 
ACCAGTCGAC AAATGATGGG TGGTTTTTTG AACGCAGCGG CCAATGGAAT ATTGGAGAAG 
CAACAACCGG CACAAAAACC AGTCGTGGTC GATACGGAGA AAGAATTAAA AGGCCAAAGT 
ATAATGGATG CCCTGCTTGG CATGTTTACA ACCAAAGTGA ATGCGGACGA GATGAAGAAG 
GAGAACTCGT CGGATAGTTT GGCCAGTGGC TCGCAAAAAT CCGCTGTGTC TAGTCCGGAG 
CAGCTGCCTT CACCCACCTC GCCCATTGCC GCTGAAATAG GCGATAAACC CTACGGACGC 
GGACGTCTGC TCTGCTTGGA TGGTGGTGGC ATTCGTGGTC TAGTCCTGGT ACAGATGCTA 
CTCGAAATAG AAAAGCTTTC TCGCACTCCT ATTATCCATA TGTTCGACTG GATTGCCGGC 
ACCAGTACTG GCGGAATTCT AGCTTTGGCG CTGGGTTGCG GCAAAACGAT GCGTCAATGC 
ATGGGCCTCT ATTTGCGCAT GAAGGAGCAG TGTTTTGTGG GCTCACGGCC CTATAACAGC 
GAGTTTTTCG AGTCCATTTT GAAGGACAAT CTGGGCGAGT TTAACGTGAT GACGGATATT 
AAGCACCCGA AGATCATGGT CACTGGTGTG ATGGCGGATC GCAAGCCGGT CGATCTACAT 
CTATTCCGCA ACTATACGAG CGCCAGTGAC ATTTTGGGCA TTGTGACTCC AATTAACAAC 
CGAAGAATTC CTCCTCCTCA GCCTTCGGAA CAATTGGTAT GGCGTGCTGC TCGGGCTACT 
GGAGCTGCGC CCTCATATTT CCGCGCATTT GGTCGCTTTC TGGACGGCGG CTTGATTGCC 
AACAACCCGA CTCTAGACGC CATGACCGAG ATTCACGAGT ACAATATGGC CTTGCGTAGC 
GCTGGACGCG AATCAGAGGC CATACCTGTA TCCGTGGTGA TGTCGCTGGG CACGGGTCAT 
ATTCCGGTGA CTGAACTTAA GGACATTGAT GTCTTCCGGC CGGAGAGCAT CTGGGATACG 
GCCAAACTGG CCTACGGAAT CTCTACCATT GGCAATCTGC TAGTGGACCA GGCCACCTGC 
TCCGATGGAA GAGTAGTGGA CAGAGCTCGC GCCTGGTGCA GCACGATTGG CATACCCTAC 
TTCAGGTTCA ATCCTCAGCT GAGCGAGGAC ATCGCCATGG ACGAAAAGGA CGACCAAAAA 
CTGATCAATA TGCTGTGGCA CACTAAGGCG TATATGCACG CCAATCGGAA TAAGATAATC 
GAGATGATCA ATTTCCTAAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729565.2
CDS	256..2919
Translation	MAWMALGALASGFVLQRLLGVDQPANKVVEIKSEALGTLQVLARDDAMLLFAPPFNSSNEKRAVYEIILQRPTSDSNTTSFSLYRSPVQQEAEERFNAFLQRLPVFVSIVKEYYNVNGLQKACDALADNPSWTLSHLIAYFNLVDYISNPKMLQCVDQADAATLMSPFQLAIKQGHMEMVKALLPLSKLEHLDINSNSVFHYAASTTKEIINLIIDKSTVNLNHLNSDGYTPLHVACLADKPENVKALLLAGANVNLNAKDIRKVYKTSAPTTVSSFLRTNVSKLYTQDMKYGGTPLHWCSSRETLHALIMEGCDVNATNFDGRTALHVMVARNRFECVVTLLAHDAEIDVLDKDGNAALHIAIEKKLVPIVQCLVVFGCDINLKNKDGKTPRHMVGNDASGNKDDEILYILHSVGAKRCKDTGSKCPPGCNAKGNYNGIPPEAPESVEQREHIEHMLATTSRQMMGGFLNAAANGILEKQQPAQKPVVVDTEKELKGQSIMDALLGMFTTKVNADEMKKENSSDSLASGSQKSAVSSPEQLPSPTSPIAAEIGDKPYGRGRLLCLDGGGIRGLVLVQMLLEIEKLSRTPIIHMFDWIAGTSTGGILALALGCGKTMRQCMGLYLRMKEQCFVGSRPYNSEFFESILKDNLGEFNVMTDIKHPKIMVTGVMADRKPVDLHLFRNYTSASDILGIVTPINNRRIPPPQPSEQLVWRAARATGAAPSYFRAFGRFLDGGLIANNPTLDAMTEIHEYNMALRSAGRESEAIPVSVVMSLGTGHIPVTELKDIDVFRPESIWDTAKLAYGISTIGNLLVDQATCSDGRVVDRARAWCSTIGIPYFRFNPQLSEDIAMDEKDDQKLINMLWHTKAYMHANRNKIIEMINFLK

Target ORF information:

RefSeq Version	NM_168383.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster calcium-independent phospholipase A2 VIA (iPLA2-VIA), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168383.2

ORF Insert Sequence:

ATGGCGTGGA TGGCGTTAGG GGCACTAGCC TCCGGTTTCG TTCTACAGCG CCTGCTGGGT 
GTCGATCAGC CGGCCAATAA GGTCGTGGAA ATTAAGAGCG AAGCGCTGGG GACACTTCAA 
GTCCTGGCCC GCGATGACGC CATGCTCCTC TTTGCACCGC CCTTCAACAG CAGCAATGAA 
AAACGAGCCG TCTATGAGAT CATTTTGCAG CGACCCACCT CGGATTCCAA TACCACATCC 
TTCAGTCTGT ACCGGTCGCC GGTGCAGCAG GAAGCCGAGG AACGCTTCAA TGCCTTTCTG 
CAAAGGCTGC CCGTTTTCGT CAGCATTGTG AAAGAGTACT ACAATGTAAA TGGGCTGCAG 
AAAGCTTGCG ATGCCTTGGC TGATAATCCC TCCTGGACGC TGTCCCATTT GATTGCCTAC 
TTCAATCTGG TGGACTACAT TAGCAATCCC AAGATGCTCC AGTGCGTGGA CCAAGCAGAT 
GCCGCCACCC TAATGTCGCC CTTCCAGTTG GCCATCAAAC AGGGTCACAT GGAGATGGTG 
AAGGCTCTGC TGCCGCTGAG CAAGCTGGAA CACTTGGATA TAAACAGCAA TTCGGTGTTT 
CATTACGCCG CCAGCACCAC CAAAGAGATT ATCAATCTTA TCATCGATAA AAGCACGGTA 
AATCTGAATC ATCTCAACTC CGATGGGTAC ACACCACTTC ATGTCGCCTG CCTCGCCGAC 
AAGCCAGAGA ATGTGAAGGC ACTGTTGCTG GCTGGGGCCA ATGTCAATCT CAATGCCAAG 
GACATCCGCA AAGTGTACAA GACATCCGCA CCGACCACGG TTTCCTCGTT TCTGCGCACC 
AATGTTAGCA AGCTGTACAC ACAGGACATG AAATATGGCG GCACTCCTCT GCACTGGTGT 
TCCTCCCGTG AAACCCTGCA TGCGCTGATC ATGGAGGGTT GTGATGTCAA CGCCACGAAC 
TTTGATGGAC GGACAGCACT GCACGTGATG GTGGCCAGGA ATCGGTTCGA GTGCGTTGTC 
ACACTACTGG CCCACGATGC GGAGATCGAT GTGCTGGACA AAGACGGAAA TGCAGCTTTG 
CACATTGCCA TTGAGAAGAA ACTAGTGCCG ATCGTCCAAT GTCTGGTGGT CTTTGGGTGT 
GACATCAATC TGAAGAACAA GGATGGCAAA ACGCCACGTC ACATGGTTGG CAATGATGCT 
AGTGGCAATA AGGATGATGA AATCCTATAC ATTCTGCACT CTGTGGGGGC TAAGCGCTGC 
AAGGATACTG GTTCAAAGTG CCCGCCTGGT TGCAATGCCA AAGGTAACTA CAACGGCATT 
CCGCCAGAGG CACCGGAATC CGTGGAGCAG CGCGAGCATA TAGAGCACAT GCTGGCCACC 
ACCAGTCGAC AAATGATGGG TGGTTTTTTG AACGCAGCGG CCAATGGAAT ATTGGAGAAG 
CAACAACCGG CACAAAAACC AGTCGTGGTC GATACGGAGA AAGAATTAAA AGGCCAAAGT 
ATAATGGATG CCCTGCTTGG CATGTTTACA ACCAAAGTGA ATGCGGACGA GATGAAGAAG 
GAGAACTCGT CGGATAGTTT GGCCAGTGGC TCGCAAAAAT CCGCTGTGTC TAGTCCGGAG 
CAGCTGCCTT CACCCACCTC GCCCATTGCC GCTGAAATAG GCGATAAACC CTACGGACGC 
GGACGTCTGC TCTGCTTGGA TGGTGGTGGC ATTCGTGGTC TAGTCCTGGT ACAGATGCTA 
CTCGAAATAG AAAAGCTTTC TCGCACTCCT ATTATCCATA TGTTCGACTG GATTGCCGGC 
ACCAGTACTG GCGGAATTCT AGCTTTGGCG CTGGGTTGCG GCAAAACGAT GCGTCAATGC 
ATGGGCCTCT ATTTGCGCAT GAAGGAGCAG TGTTTTGTGG GCTCACGGCC CTATAACAGC 
GAGTTTTTCG AGTCCATTTT GAAGGACAAT CTGGGCGAGT TTAACGTGAT GACGGATATT 
AAGCACCCGA AGATCATGGT CACTGGTGTG ATGGCGGATC GCAAGCCGGT CGATCTACAT 
CTATTCCGCA ACTATACGAG CGCCAGTGAC ATTTTGGGCA TTGTGACTCC AATTAACAAC 
CGAAGAATTC CTCCTCCTCA GCCTTCGGAA CAATTGGTAT GGCGTGCTGC TCGGGCTACT 
GGAGCTGCGC CCTCATATTT CCGCGCATTT GGTCGCTTTC TGGACGGCGG CTTGATTGCC 
AACAACCCGA CTCTAGACGC CATGACCGAG ATTCACGAGT ACAATATGGC CTTGCGTAGC 
GCTGGACGCG AATCAGAGGC CATACCTGTA TCCGTGGTGA TGTCGCTGGG CACGGGTCAT 
ATTCCGGTGA CTGAACTTAA GGACATTGAT GTCTTCCGGC CGGAGAGCAT CTGGGATACG 
GCCAAACTGG CCTACGGAAT CTCTACCATT GGCAATCTGC TAGTGGACCA GGCCACCTGC 
TCCGATGGAA GAGTAGTGGA CAGAGCTCGC GCCTGGTGCA GCACGATTGG CATACCCTAC 
TTCAGGTTCA ATCCTCAGCT GAGCGAGGAC ATCGCCATGG ACGAAAAGGA CGACCAAAAA 
CTGATCAATA TGCTGTGGCA CACTAAGGCG TATATGCACG CCAATCGGAA TAAGATAATC 
GAGATGATCA ATTTCCTAAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

iPLA2-VIA ( NM_168385.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729567.2 Drosophila melanogaster calcium-independent phospholipase A2 VIA (iPLA2-VIA), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa18123
Clone ID Related Accession (Same CDS sequence)	NM_168384.2 , NM_168383.2 , NM_168385.2
Accession Version	NM_168385.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	calcium-independent phospholipase A2 VIA, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_168385.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGTGGA TGGCGTTAGG GGCACTAGCC TCCGGTTTCG TTCTACAGCG CCTGCTGGGT 
GTCGATCAGC CGGCCAATAA GGTCGTGGAA ATTAAGAGCG AAGCGCTGGG GACACTTCAA 
GTCCTGGCCC GCGATGACGC CATGCTCCTC TTTGCACCGC CCTTCAACAG CAGCAATGAA 
AAACGAGCCG TCTATGAGAT CATTTTGCAG CGACCCACCT CGGATTCCAA TACCACATCC 
TTCAGTCTGT ACCGGTCGCC GGTGCAGCAG GAAGCCGAGG AACGCTTCAA TGCCTTTCTG 
CAAAGGCTGC CCGTTTTCGT CAGCATTGTG AAAGAGTACT ACAATGTAAA TGGGCTGCAG 
AAAGCTTGCG ATGCCTTGGC TGATAATCCC TCCTGGACGC TGTCCCATTT GATTGCCTAC 
TTCAATCTGG TGGACTACAT TAGCAATCCC AAGATGCTCC AGTGCGTGGA CCAAGCAGAT 
GCCGCCACCC TAATGTCGCC CTTCCAGTTG GCCATCAAAC AGGGTCACAT GGAGATGGTG 
AAGGCTCTGC TGCCGCTGAG CAAGCTGGAA CACTTGGATA TAAACAGCAA TTCGGTGTTT 
CATTACGCCG CCAGCACCAC CAAAGAGATT ATCAATCTTA TCATCGATAA AAGCACGGTA 
AATCTGAATC ATCTCAACTC CGATGGGTAC ACACCACTTC ATGTCGCCTG CCTCGCCGAC 
AAGCCAGAGA ATGTGAAGGC ACTGTTGCTG GCTGGGGCCA ATGTCAATCT CAATGCCAAG 
GACATCCGCA AAGTGTACAA GACATCCGCA CCGACCACGG TTTCCTCGTT TCTGCGCACC 
AATGTTAGCA AGCTGTACAC ACAGGACATG AAATATGGCG GCACTCCTCT GCACTGGTGT 
TCCTCCCGTG AAACCCTGCA TGCGCTGATC ATGGAGGGTT GTGATGTCAA CGCCACGAAC 
TTTGATGGAC GGACAGCACT GCACGTGATG GTGGCCAGGA ATCGGTTCGA GTGCGTTGTC 
ACACTACTGG CCCACGATGC GGAGATCGAT GTGCTGGACA AAGACGGAAA TGCAGCTTTG 
CACATTGCCA TTGAGAAGAA ACTAGTGCCG ATCGTCCAAT GTCTGGTGGT CTTTGGGTGT 
GACATCAATC TGAAGAACAA GGATGGCAAA ACGCCACGTC ACATGGTTGG CAATGATGCT 
AGTGGCAATA AGGATGATGA AATCCTATAC ATTCTGCACT CTGTGGGGGC TAAGCGCTGC 
AAGGATACTG GTTCAAAGTG CCCGCCTGGT TGCAATGCCA AAGGTAACTA CAACGGCATT 
CCGCCAGAGG CACCGGAATC CGTGGAGCAG CGCGAGCATA TAGAGCACAT GCTGGCCACC 
ACCAGTCGAC AAATGATGGG TGGTTTTTTG AACGCAGCGG CCAATGGAAT ATTGGAGAAG 
CAACAACCGG CACAAAAACC AGTCGTGGTC GATACGGAGA AAGAATTAAA AGGCCAAAGT 
ATAATGGATG CCCTGCTTGG CATGTTTACA ACCAAAGTGA ATGCGGACGA GATGAAGAAG 
GAGAACTCGT CGGATAGTTT GGCCAGTGGC TCGCAAAAAT CCGCTGTGTC TAGTCCGGAG 
CAGCTGCCTT CACCCACCTC GCCCATTGCC GCTGAAATAG GCGATAAACC CTACGGACGC 
GGACGTCTGC TCTGCTTGGA TGGTGGTGGC ATTCGTGGTC TAGTCCTGGT ACAGATGCTA 
CTCGAAATAG AAAAGCTTTC TCGCACTCCT ATTATCCATA TGTTCGACTG GATTGCCGGC 
ACCAGTACTG GCGGAATTCT AGCTTTGGCG CTGGGTTGCG GCAAAACGAT GCGTCAATGC 
ATGGGCCTCT ATTTGCGCAT GAAGGAGCAG TGTTTTGTGG GCTCACGGCC CTATAACAGC 
GAGTTTTTCG AGTCCATTTT GAAGGACAAT CTGGGCGAGT TTAACGTGAT GACGGATATT 
AAGCACCCGA AGATCATGGT CACTGGTGTG ATGGCGGATC GCAAGCCGGT CGATCTACAT 
CTATTCCGCA ACTATACGAG CGCCAGTGAC ATTTTGGGCA TTGTGACTCC AATTAACAAC 
CGAAGAATTC CTCCTCCTCA GCCTTCGGAA CAATTGGTAT GGCGTGCTGC TCGGGCTACT 
GGAGCTGCGC CCTCATATTT CCGCGCATTT GGTCGCTTTC TGGACGGCGG CTTGATTGCC 
AACAACCCGA CTCTAGACGC CATGACCGAG ATTCACGAGT ACAATATGGC CTTGCGTAGC 
GCTGGACGCG AATCAGAGGC CATACCTGTA TCCGTGGTGA TGTCGCTGGG CACGGGTCAT 
ATTCCGGTGA CTGAACTTAA GGACATTGAT GTCTTCCGGC CGGAGAGCAT CTGGGATACG 
GCCAAACTGG CCTACGGAAT CTCTACCATT GGCAATCTGC TAGTGGACCA GGCCACCTGC 
TCCGATGGAA GAGTAGTGGA CAGAGCTCGC GCCTGGTGCA GCACGATTGG CATACCCTAC 
TTCAGGTTCA ATCCTCAGCT GAGCGAGGAC ATCGCCATGG ACGAAAAGGA CGACCAAAAA 
CTGATCAATA TGCTGTGGCA CACTAAGGCG TATATGCACG CCAATCGGAA TAAGATAATC 
GAGATGATCA ATTTCCTAAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729567.2
CDS	441..3104
Translation	MAWMALGALASGFVLQRLLGVDQPANKVVEIKSEALGTLQVLARDDAMLLFAPPFNSSNEKRAVYEIILQRPTSDSNTTSFSLYRSPVQQEAEERFNAFLQRLPVFVSIVKEYYNVNGLQKACDALADNPSWTLSHLIAYFNLVDYISNPKMLQCVDQADAATLMSPFQLAIKQGHMEMVKALLPLSKLEHLDINSNSVFHYAASTTKEIINLIIDKSTVNLNHLNSDGYTPLHVACLADKPENVKALLLAGANVNLNAKDIRKVYKTSAPTTVSSFLRTNVSKLYTQDMKYGGTPLHWCSSRETLHALIMEGCDVNATNFDGRTALHVMVARNRFECVVTLLAHDAEIDVLDKDGNAALHIAIEKKLVPIVQCLVVFGCDINLKNKDGKTPRHMVGNDASGNKDDEILYILHSVGAKRCKDTGSKCPPGCNAKGNYNGIPPEAPESVEQREHIEHMLATTSRQMMGGFLNAAANGILEKQQPAQKPVVVDTEKELKGQSIMDALLGMFTTKVNADEMKKENSSDSLASGSQKSAVSSPEQLPSPTSPIAAEIGDKPYGRGRLLCLDGGGIRGLVLVQMLLEIEKLSRTPIIHMFDWIAGTSTGGILALALGCGKTMRQCMGLYLRMKEQCFVGSRPYNSEFFESILKDNLGEFNVMTDIKHPKIMVTGVMADRKPVDLHLFRNYTSASDILGIVTPINNRRIPPPQPSEQLVWRAARATGAAPSYFRAFGRFLDGGLIANNPTLDAMTEIHEYNMALRSAGRESEAIPVSVVMSLGTGHIPVTELKDIDVFRPESIWDTAKLAYGISTIGNLLVDQATCSDGRVVDRARAWCSTIGIPYFRFNPQLSEDIAMDEKDDQKLINMLWHTKAYMHANRNKIIEMINFLK

Target ORF information:

RefSeq Version	NM_168385.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster calcium-independent phospholipase A2 VIA (iPLA2-VIA), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168385.2

ORF Insert Sequence:

ATGGCGTGGA TGGCGTTAGG GGCACTAGCC TCCGGTTTCG TTCTACAGCG CCTGCTGGGT 
GTCGATCAGC CGGCCAATAA GGTCGTGGAA ATTAAGAGCG AAGCGCTGGG GACACTTCAA 
GTCCTGGCCC GCGATGACGC CATGCTCCTC TTTGCACCGC CCTTCAACAG CAGCAATGAA 
AAACGAGCCG TCTATGAGAT CATTTTGCAG CGACCCACCT CGGATTCCAA TACCACATCC 
TTCAGTCTGT ACCGGTCGCC GGTGCAGCAG GAAGCCGAGG AACGCTTCAA TGCCTTTCTG 
CAAAGGCTGC CCGTTTTCGT CAGCATTGTG AAAGAGTACT ACAATGTAAA TGGGCTGCAG 
AAAGCTTGCG ATGCCTTGGC TGATAATCCC TCCTGGACGC TGTCCCATTT GATTGCCTAC 
TTCAATCTGG TGGACTACAT TAGCAATCCC AAGATGCTCC AGTGCGTGGA CCAAGCAGAT 
GCCGCCACCC TAATGTCGCC CTTCCAGTTG GCCATCAAAC AGGGTCACAT GGAGATGGTG 
AAGGCTCTGC TGCCGCTGAG CAAGCTGGAA CACTTGGATA TAAACAGCAA TTCGGTGTTT 
CATTACGCCG CCAGCACCAC CAAAGAGATT ATCAATCTTA TCATCGATAA AAGCACGGTA 
AATCTGAATC ATCTCAACTC CGATGGGTAC ACACCACTTC ATGTCGCCTG CCTCGCCGAC 
AAGCCAGAGA ATGTGAAGGC ACTGTTGCTG GCTGGGGCCA ATGTCAATCT CAATGCCAAG 
GACATCCGCA AAGTGTACAA GACATCCGCA CCGACCACGG TTTCCTCGTT TCTGCGCACC 
AATGTTAGCA AGCTGTACAC ACAGGACATG AAATATGGCG GCACTCCTCT GCACTGGTGT 
TCCTCCCGTG AAACCCTGCA TGCGCTGATC ATGGAGGGTT GTGATGTCAA CGCCACGAAC 
TTTGATGGAC GGACAGCACT GCACGTGATG GTGGCCAGGA ATCGGTTCGA GTGCGTTGTC 
ACACTACTGG CCCACGATGC GGAGATCGAT GTGCTGGACA AAGACGGAAA TGCAGCTTTG 
CACATTGCCA TTGAGAAGAA ACTAGTGCCG ATCGTCCAAT GTCTGGTGGT CTTTGGGTGT 
GACATCAATC TGAAGAACAA GGATGGCAAA ACGCCACGTC ACATGGTTGG CAATGATGCT 
AGTGGCAATA AGGATGATGA AATCCTATAC ATTCTGCACT CTGTGGGGGC TAAGCGCTGC 
AAGGATACTG GTTCAAAGTG CCCGCCTGGT TGCAATGCCA AAGGTAACTA CAACGGCATT 
CCGCCAGAGG CACCGGAATC CGTGGAGCAG CGCGAGCATA TAGAGCACAT GCTGGCCACC 
ACCAGTCGAC AAATGATGGG TGGTTTTTTG AACGCAGCGG CCAATGGAAT ATTGGAGAAG 
CAACAACCGG CACAAAAACC AGTCGTGGTC GATACGGAGA AAGAATTAAA AGGCCAAAGT 
ATAATGGATG CCCTGCTTGG CATGTTTACA ACCAAAGTGA ATGCGGACGA GATGAAGAAG 
GAGAACTCGT CGGATAGTTT GGCCAGTGGC TCGCAAAAAT CCGCTGTGTC TAGTCCGGAG 
CAGCTGCCTT CACCCACCTC GCCCATTGCC GCTGAAATAG GCGATAAACC CTACGGACGC 
GGACGTCTGC TCTGCTTGGA TGGTGGTGGC ATTCGTGGTC TAGTCCTGGT ACAGATGCTA 
CTCGAAATAG AAAAGCTTTC TCGCACTCCT ATTATCCATA TGTTCGACTG GATTGCCGGC 
ACCAGTACTG GCGGAATTCT AGCTTTGGCG CTGGGTTGCG GCAAAACGAT GCGTCAATGC 
ATGGGCCTCT ATTTGCGCAT GAAGGAGCAG TGTTTTGTGG GCTCACGGCC CTATAACAGC 
GAGTTTTTCG AGTCCATTTT GAAGGACAAT CTGGGCGAGT TTAACGTGAT GACGGATATT 
AAGCACCCGA AGATCATGGT CACTGGTGTG ATGGCGGATC GCAAGCCGGT CGATCTACAT 
CTATTCCGCA ACTATACGAG CGCCAGTGAC ATTTTGGGCA TTGTGACTCC AATTAACAAC 
CGAAGAATTC CTCCTCCTCA GCCTTCGGAA CAATTGGTAT GGCGTGCTGC TCGGGCTACT 
GGAGCTGCGC CCTCATATTT CCGCGCATTT GGTCGCTTTC TGGACGGCGG CTTGATTGCC 
AACAACCCGA CTCTAGACGC CATGACCGAG ATTCACGAGT ACAATATGGC CTTGCGTAGC 
GCTGGACGCG AATCAGAGGC CATACCTGTA TCCGTGGTGA TGTCGCTGGG CACGGGTCAT 
ATTCCGGTGA CTGAACTTAA GGACATTGAT GTCTTCCGGC CGGAGAGCAT CTGGGATACG 
GCCAAACTGG CCTACGGAAT CTCTACCATT GGCAATCTGC TAGTGGACCA GGCCACCTGC 
TCCGATGGAA GAGTAGTGGA CAGAGCTCGC GCCTGGTGCA GCACGATTGG CATACCCTAC 
TTCAGGTTCA ATCCTCAGCT GAGCGAGGAC ATCGCCATGG ACGAAAAGGA CGACCAAAAA 
CTGATCAATA TGCTGTGGCA CACTAAGGCG TATATGCACG CCAATCGGAA TAAGATAATC 
GAGATGATCA ATTTCCTAAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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iPLA2-VIA cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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