Ubc4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ubc4
Entrez Gene ID	39133
Full Name	Ubiquitin conjugating enzyme 4
Synonyms	CG8284,Dmel\CG8284,E2,UbcD4,UbcD4 E2
General protein information	Preferred Names Ubiquitin conjugating enzyme 4 Names CG8284 gene product from transcript CG8284-RC CG8284-PA CG8284-PB CG8284-PC Ubc4-PA Ubc4-PB Ubc4-PC ubiquitin conjugating enzyme 4
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 67C5-67C5

mRNA and Protein(s)

mRNA	Protein	Name
NM_079286.3	NP_524010.2	ubiquitin conjugating enzyme 4, isoform A
NM_001300084.1	NP_001287013.1	ubiquitin conjugating enzyme 4, isoform B
NM_001300085.1	NP_001287014.1	ubiquitin conjugating enzyme 4, isoform C

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ube2k	NP_001099476.1
Oryza sativa (rice)	Os01g0925800	NP_001045259.1
Xenopus tropicalis (tropical clawed frog)	ube2k	NP_001005662.1
Danio rerio (zebrafish)	ube2ka	NP_001013500.1
Drosophila melanogaster (fruit fly)	UbcD4	NP_524010.2
Caenorhabditis elegans (roundworm)	ubc-21	NP_509502.3
Saccharomyces cerevisiae (baker's yeast)	UBC1	NP_010462.3
Arabidopsis thaliana (thale cress)	UBC27	NP_001190508.1
Pan troglodytes (chimpanzee)	UBE2K	NP_001233454.1
Canis lupus familiaris (dog)	UBE2K	XP_003434442.1
Mus musculus (house mouse)	Ube2k	NP_058066.2
Kluyveromyces lactis	KLLA0C18656g	XP_453031.1
Magnaporthe oryzae (rice blast fungus)	MGG_05478	XP_003710343.1
Homo sapiens (human)	UBE2K	NP_005330.1
	UBE2K	XP_001093369.1
Bos taurus (cattle)	UBE2K	NP_776505.1
Gallus gallus (chicken)	UBE2K	NP_001026711.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004889	XP_314290.2
Danio rerio (zebrafish)	ube2kb	NP_001008611.1
Caenorhabditis elegans (roundworm)	ubc-20	NP_497174.1
Eremothecium gossypii	AGOS_ADR169C	NP_984266.1
Schizosaccharomyces pombe (fission yeast)	ubc1	NP_596239.1
Neurospora crassa	NCU01225	XP_961591.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We found that UbcD4, in addition to the previously reported E2s Effete and Bendless, was required for activation of the IMD pathway.
Title: UbcD4, an ortholog of E2-25K/Ube2K, is essential for activation of the immune deficiency pathway in Drosophila.

The following Ubc4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ubc4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18081	NM_079286.3 Latest version!	Drosophila melanogaster ubiquitin conjugating enzyme 4, transcript variant A (Ubc4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa18081	NM_001300084.1 Latest version!	Drosophila melanogaster ubiquitin conjugating enzyme 4, transcript variant B (Ubc4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa18081	NM_001300085.1 Latest version!	Drosophila melanogaster ubiquitin conjugating enzyme 4, transcript variant C (Ubc4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ubc4 ( NM_079286.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524010.2 Drosophila melanogaster ubiquitin conjugating enzyme 4, transcript variant A (Ubc4), mRNA.

Price & Availability↑

CloneID	OFa18081
Clone ID Related Accession (Same CDS sequence)	NM_079286.3 , NM_001300084.1 , NM_001300085.1
Accession Version	NM_079286.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ubiquitin conjugating enzyme 4, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079286.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGAACA TGGCAGTGTC GCGGATCAAG CGGGAGTTCA AGGAGGTGAT GCGCAGCGAG 
GAGATCGTCC AGTGTTCCAT CAAAATCGAA CTGGTCAATG ACAGTTGGAC GGAGCTGCGC 
GGCGAGATCG CCGGTCCGCC TGACACGCCC TACGAGGGCG GCAAGTTCGT CCTGGAGATC 
AAGGTGCCCG AGACATATCC CTTCAATCCA CCAAAGGTAC GCTTCATAAC GCGCATCTGG 
CATCCGAATA TATCGTCGGT TACGGGTGCG ATTTGCTTGG ACATCTTAAA GGACAACTGG 
GCTGCAGCGA TGACACTGCG CACCGTACTG CTGTCGCTGC AGGCGCTCCT GGCCGCCGCA 
GAGCCGGACG ATCCACAGGA CGCAGTGGTG GCCTATCAGT TTAAGGACAA GTACGATCTG 
TTCCTGCTTA CGGCCAAGCA CTGGACCAAC GCGTATGCGG GCGGACCGCA CACCTTTCCC 
GATTGTGATT CAAAGATCCA ACGTCTCAGG GACATGGGCA TCGACGAGCA TGAGGCGCGC 
GCCGTGCTCT CCAAGGAGAA TTGGAATTTG GAGAAGGCCA CCGAGGGCCT GTTCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524010.2
CDS	358..957
Translation	MANMAVSRIKREFKEVMRSEEIVQCSIKIELVNDSWTELRGEIAGPPDTPYEGGKFVLEIKVPETYPFNPPKVRFITRIWHPNISSVTGAICLDILKDNWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVAYQFKDKYDLFLLTAKHWTNAYAGGPHTFPDCDSKIQRLRDMGIDEHEARAVLSKENWNLEKATEGLFS

Target ORF information:

RefSeq Version	NM_079286.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ubiquitin conjugating enzyme 4, transcript variant A (Ubc4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079286.3

ORF Insert Sequence:

ATGGCGAACA TGGCAGTGTC GCGGATCAAG CGGGAGTTCA AGGAGGTGAT GCGCAGCGAG 
GAGATCGTCC AGTGTTCCAT CAAAATCGAA CTGGTCAATG ACAGTTGGAC GGAGCTGCGC 
GGCGAGATCG CCGGTCCGCC TGACACGCCC TACGAGGGCG GCAAGTTCGT CCTGGAGATC 
AAGGTGCCCG AGACATATCC CTTCAATCCA CCAAAGGTAC GCTTCATAAC GCGCATCTGG 
CATCCGAATA TATCGTCGGT TACGGGTGCG ATTTGCTTGG ACATCTTAAA GGACAACTGG 
GCTGCAGCGA TGACACTGCG CACCGTACTG CTGTCGCTGC AGGCGCTCCT GGCCGCCGCA 
GAGCCGGACG ATCCACAGGA CGCAGTGGTG GCCTATCAGT TTAAGGACAA GTACGATCTG 
TTCCTGCTTA CGGCCAAGCA CTGGACCAAC GCGTATGCGG GCGGACCGCA CACCTTTCCC 
GATTGTGATT CAAAGATCCA ACGTCTCAGG GACATGGGCA TCGACGAGCA TGAGGCGCGC 
GCCGTGCTCT CCAAGGAGAA TTGGAATTTG GAGAAGGCCA CCGAGGGCCT GTTCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ubc4 ( NM_001300084.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287013.1 Drosophila melanogaster ubiquitin conjugating enzyme 4, transcript variant B (Ubc4), mRNA.

Price & Availability↑

CloneID	OFa18081
Clone ID Related Accession (Same CDS sequence)	NM_079286.3 , NM_001300084.1 , NM_001300085.1
Accession Version	NM_001300084.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ubiquitin conjugating enzyme 4, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGAACA TGGCAGTGTC GCGGATCAAG CGGGAGTTCA AGGAGGTGAT GCGCAGCGAG 
GAGATCGTCC AGTGTTCCAT CAAAATCGAA CTGGTCAATG ACAGTTGGAC GGAGCTGCGC 
GGCGAGATCG CCGGTCCGCC TGACACGCCC TACGAGGGCG GCAAGTTCGT CCTGGAGATC 
AAGGTGCCCG AGACATATCC CTTCAATCCA CCAAAGGTAC GCTTCATAAC GCGCATCTGG 
CATCCGAATA TATCGTCGGT TACGGGTGCG ATTTGCTTGG ACATCTTAAA GGACAACTGG 
GCTGCAGCGA TGACACTGCG CACCGTACTG CTGTCGCTGC AGGCGCTCCT GGCCGCCGCA 
GAGCCGGACG ATCCACAGGA CGCAGTGGTG GCCTATCAGT TTAAGGACAA GTACGATCTG 
TTCCTGCTTA CGGCCAAGCA CTGGACCAAC GCGTATGCGG GCGGACCGCA CACCTTTCCC 
GATTGTGATT CAAAGATCCA ACGTCTCAGG GACATGGGCA TCGACGAGCA TGAGGCGCGC 
GCCGTGCTCT CCAAGGAGAA TTGGAATTTG GAGAAGGCCA CCGAGGGCCT GTTCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287013.1
CDS	358..957
Translation	MANMAVSRIKREFKEVMRSEEIVQCSIKIELVNDSWTELRGEIAGPPDTPYEGGKFVLEIKVPETYPFNPPKVRFITRIWHPNISSVTGAICLDILKDNWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVAYQFKDKYDLFLLTAKHWTNAYAGGPHTFPDCDSKIQRLRDMGIDEHEARAVLSKENWNLEKATEGLFS

Target ORF information:

RefSeq Version	NM_001300084.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ubiquitin conjugating enzyme 4, transcript variant B (Ubc4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300084.1

ORF Insert Sequence:

ATGGCGAACA TGGCAGTGTC GCGGATCAAG CGGGAGTTCA AGGAGGTGAT GCGCAGCGAG 
GAGATCGTCC AGTGTTCCAT CAAAATCGAA CTGGTCAATG ACAGTTGGAC GGAGCTGCGC 
GGCGAGATCG CCGGTCCGCC TGACACGCCC TACGAGGGCG GCAAGTTCGT CCTGGAGATC 
AAGGTGCCCG AGACATATCC CTTCAATCCA CCAAAGGTAC GCTTCATAAC GCGCATCTGG 
CATCCGAATA TATCGTCGGT TACGGGTGCG ATTTGCTTGG ACATCTTAAA GGACAACTGG 
GCTGCAGCGA TGACACTGCG CACCGTACTG CTGTCGCTGC AGGCGCTCCT GGCCGCCGCA 
GAGCCGGACG ATCCACAGGA CGCAGTGGTG GCCTATCAGT TTAAGGACAA GTACGATCTG 
TTCCTGCTTA CGGCCAAGCA CTGGACCAAC GCGTATGCGG GCGGACCGCA CACCTTTCCC 
GATTGTGATT CAAAGATCCA ACGTCTCAGG GACATGGGCA TCGACGAGCA TGAGGCGCGC 
GCCGTGCTCT CCAAGGAGAA TTGGAATTTG GAGAAGGCCA CCGAGGGCCT GTTCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ubc4 ( NM_001300085.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287014.1 Drosophila melanogaster ubiquitin conjugating enzyme 4, transcript variant C (Ubc4), mRNA.

Price & Availability↑

CloneID	OFa18081
Clone ID Related Accession (Same CDS sequence)	NM_079286.3 , NM_001300084.1 , NM_001300085.1
Accession Version	NM_001300085.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ubiquitin conjugating enzyme 4, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGAACA TGGCAGTGTC GCGGATCAAG CGGGAGTTCA AGGAGGTGAT GCGCAGCGAG 
GAGATCGTCC AGTGTTCCAT CAAAATCGAA CTGGTCAATG ACAGTTGGAC GGAGCTGCGC 
GGCGAGATCG CCGGTCCGCC TGACACGCCC TACGAGGGCG GCAAGTTCGT CCTGGAGATC 
AAGGTGCCCG AGACATATCC CTTCAATCCA CCAAAGGTAC GCTTCATAAC GCGCATCTGG 
CATCCGAATA TATCGTCGGT TACGGGTGCG ATTTGCTTGG ACATCTTAAA GGACAACTGG 
GCTGCAGCGA TGACACTGCG CACCGTACTG CTGTCGCTGC AGGCGCTCCT GGCCGCCGCA 
GAGCCGGACG ATCCACAGGA CGCAGTGGTG GCCTATCAGT TTAAGGACAA GTACGATCTG 
TTCCTGCTTA CGGCCAAGCA CTGGACCAAC GCGTATGCGG GCGGACCGCA CACCTTTCCC 
GATTGTGATT CAAAGATCCA ACGTCTCAGG GACATGGGCA TCGACGAGCA TGAGGCGCGC 
GCCGTGCTCT CCAAGGAGAA TTGGAATTTG GAGAAGGCCA CCGAGGGCCT GTTCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287014.1
CDS	358..957
Translation	MANMAVSRIKREFKEVMRSEEIVQCSIKIELVNDSWTELRGEIAGPPDTPYEGGKFVLEIKVPETYPFNPPKVRFITRIWHPNISSVTGAICLDILKDNWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVAYQFKDKYDLFLLTAKHWTNAYAGGPHTFPDCDSKIQRLRDMGIDEHEARAVLSKENWNLEKATEGLFS

Target ORF information:

RefSeq Version	NM_001300085.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ubiquitin conjugating enzyme 4, transcript variant C (Ubc4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300085.1

ORF Insert Sequence:

ATGGCGAACA TGGCAGTGTC GCGGATCAAG CGGGAGTTCA AGGAGGTGAT GCGCAGCGAG 
GAGATCGTCC AGTGTTCCAT CAAAATCGAA CTGGTCAATG ACAGTTGGAC GGAGCTGCGC 
GGCGAGATCG CCGGTCCGCC TGACACGCCC TACGAGGGCG GCAAGTTCGT CCTGGAGATC 
AAGGTGCCCG AGACATATCC CTTCAATCCA CCAAAGGTAC GCTTCATAAC GCGCATCTGG 
CATCCGAATA TATCGTCGGT TACGGGTGCG ATTTGCTTGG ACATCTTAAA GGACAACTGG 
GCTGCAGCGA TGACACTGCG CACCGTACTG CTGTCGCTGC AGGCGCTCCT GGCCGCCGCA 
GAGCCGGACG ATCCACAGGA CGCAGTGGTG GCCTATCAGT TTAAGGACAA GTACGATCTG 
TTCCTGCTTA CGGCCAAGCA CTGGACCAAC GCGTATGCGG GCGGACCGCA CACCTTTCCC 
GATTGTGATT CAAAGATCCA ACGTCTCAGG GACATGGGCA TCGACGAGCA TGAGGCGCGC 
GCCGTGCTCT CCAAGGAGAA TTGGAATTTG GAGAAGGCCA CCGAGGGCCT GTTCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ubc4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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