LanB2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LanB2
Entrez Gene ID	39118
Full Name	Laminin B2
Synonyms	CG3322,CT11145,DROLAMB2A,Dmel\CG3322,LAMB2A,LM-B2/gamma,LMG1,Lam-B2,anon-EST:fe2H3,gamma1,knod,l(3)67BDb,lamB2,lanB2
General protein information	Preferred Names Laminin B2 Names CG3322 gene product from transcript CG3322-RA CG3322-PA CG3322-PB LamininB2 LanB2-PA LanB2-PB anon-fast-evolving-2H3 knodel laminin laminin B2 laminin B2 subunit laminin gamma laminin gamma 1 laminin gamma-chain laminin gamma1 laminin gamma2 lamininB lethal (3) 67BDb
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 67B1-67C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_079282.2	NP_524006.1	laminin B2, isoform A
NM_001300080.1	NP_001287009.1	laminin B2, isoform B

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	lamc1	NP_775384.1
Xenopus tropicalis (tropical clawed frog)	lamc1	NP_001090659.1
Homo sapiens (human)	LAMC1	NP_002284.3
Canis lupus familiaris (dog)	LAMC1	XP_537156.3
Rattus norvegicus (Norway rat)	Lamc1	NP_446418.1
Anopheles gambiae (African malaria mosquito)	LANB2	XP_308240.3
Pan troglodytes (chimpanzee)	LAMC1	XP_001162648.1
Bos taurus (cattle)	LAMC1	NP_001193746.1
Caenorhabditis elegans (roundworm)	lam-2	NP_509204.3
	LOC715265	XP_002808304.1
Mus musculus (house mouse)	Lamc1	NP_034813.2
Gallus gallus (chicken)	LAMC1	XP_001234659.2
Drosophila melanogaster (fruit fly)	LanB2	NP_524006.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonstrated that loss of the laminin gamma subunit (LanB2) in the peripheral glia of Drosophila melanogaster results in the disruption of glial morphology due to disruption of laminin secretion
Title: Accumulation of Laminin Monomers in Drosophila Glia Leads to Glial Endoplasmic Reticulum Stress and Disrupted Larval Locomotion.

The data, concerning the different function of laminin B2 during organogenesis, suggest that laminin B2 might act in a cumulative way and probably at multiple steps during extracellular matrix assembly.
Title: The role of LamininB2 (LanB2) during mesoderm differentiation in Drosophila.

The following LanB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LanB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18066	NM_001300080.1 Latest version!	Drosophila melanogaster laminin B2, transcript variant B (LanB2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa18066	NM_079282.2 Latest version!	Drosophila melanogaster laminin B2, transcript variant A (LanB2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LanB2 ( NM_079282.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524006.1 Drosophila melanogaster laminin B2, transcript variant A (LanB2), mRNA.

Price & Availability↑

CloneID	OFa18066
Clone ID Related Accession (Same CDS sequence)	NM_079282.2 , NM_001300080.1
Accession Version	NM_079282.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	laminin B2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079282.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCGCA GCCGGTGGAG CCACAGTGGC TCCTCCACGG CTCGACTCCT GCTGATCGGA 
GTATTGTTCG CCAGCTGTTC CACCGCCATC CTGGGTGCCC AGCGTCCCCC CATCAACTCC 
GCCGGAGGTC ACGAACTGCG CGGAACCACC TTCATGCCGG CCCTGGAGTG CTACGATCCA 
TACGGCAGGC CACAGAAATG TCTGCCAGAA TTTATCAATG CTGCCTATCA ACTGCAAATT 
GAGTCAACTA ATACCTGTGG TGAGCAGAAT GACAACCACT TCTGCATACA AACCATGAAC 
CAAAATCACA AAAACTGCGA ATTTTGCAAA TACAATGATC ATAATCCATC CTTCTTGACG 
GATTTGCATG ATCCGCAAAG TCCAACGTGG TGGCAATCGG AGACCATGTT CGAGGGCATT 
CAGCACCCGA ACTATGTGAA TCTGACTTTG CACCTTGGAA AATCCTATGA CATCACCTAC 
GTGCGCATTC TCTTCCGCTC ACCAAGACCC GAATCCTTTA CGATTTACAA GAGGACCTCG 
GAGAGTGGAC CCTGGATTCC CTACCAGTTC TACAGTGCCA CCTGTCGCGA CACCTACTCC 
CTGCCAGATT CGCGAGCCAT TCGCAAGGGT GAGGGCGAGG CCCATGCTCT GTGTACCAGC 
GAATACAGTG ATATCTCGCC GTTGAGGGAC GGTGAGATTG CCTTCTCCAC GCTGGAGGGT 
CGTCCCAGTG GCATCAATTT TGAGCGCAGC GGAGAACTGC AGGAGTGGGT CACGGCCACG 
GATATCCGTA TCACGCTGGA CCGACTGAAC ACCTTCGGTG ACGAACTCTT CGGTGATTCC 
CAGGTGCTCA AGTCGTACTT CTATGCCATC AGTGACATTG CCGTGGGTGC GCGTTGCAAG 
TGCAATGGAC ATGCCAGCAA GTGTGTACCG AGCACGGGAA TGCATGGCGA GAGGACTCTG 
GTCTGCGAGT GCCGGCACAA TACGGATGGA CCCGATTGCG ATCGCTGTCT GCCACTCTAC 
AACGACCTCA AGTGGAAGAG ATCCACCTCA ACGGAGGTCA ACGAGTGCAA AGCTTGCAAC 
TGCAATGGAT TGGCGGACAA GTGCTTCTTC GACGCGAATC TGTTCAATCG GACGGGTCAT 
GGAGGTCACT GCCTGGACTG CCGCGAGAAT CGCGATGGAC CCAACTGCGA ACGCTGCAAG 
GAGAACTTCT ATATGCGCGA CGATGGCTAC TGCGTCAACT GCGCCTGCGA TCCCGTTGGC 
TCCAGATCGC TGCAGTGCAA CAGCCACGGC AAGTGCCAGT GTAAGCCGGG TGTGACTGGC 
GACAAGTGCG ACCGCTGCGA TAACAACTAC TACCAATTCG GTCCCCATGG ATGCCAGCAG 
TGCGGATGCG ACAGTGGGGG ATCCCATCAG AATACACCCG CCTGCGATAC CGAGACTGGA 
ATCTGCTTCT GCAAGGAGAA TGTGGAGGGC AGACGCTGCA ATGAATGCAA GCCAGGCTTC 
TTCAATCTGG ACAAGAACAA TCGATTTGGT TGCACACCCT GCTTCTGCTA TGGGCACACC 
TCCGAGTGCA TGACTGCCCC AGGATACTCC ATTGTTTCGG TCACCTCCAA TTTTAACAAA 
TTCAAGGAAC GCTGGACGGC CGCCGATTTG AATCAACGCG AGGTGGACAT CAAGTACAAC 
CAGTATAGCC GAAGCATTGG AACCACCGCT CAGGGAAATG AGCACGTCTA CTTCCAGGCA 
CCGGATCGCT TCCTCGGCGA TCAGCGTGCC TCCTACAACA GGGATCTGAA ATTCAAGCTC 
CAGCTAGTTG GTCAGGTGGC CAATACCGGA GTGAGTGACG TGATCTTGGA GGGTGCTGGC 
AGCCGCATCT CCCTGCCCAT CTTCGCCCAG GGCAATGGAA TACCCGACCA GGGAGTTAAG 
GAGTATACCT TCCGTCTGCA TGAACATCAT GATTACCAGT GGCAACCAAG CCAGTCGGCT 
CGTGGATTCC TTTCGATTCT GTCCAATCTG ACGGCCATTA AGATCAGGGC CACGTACTCG 
GTGCAGGGTG AGGCCATCCT GGATGATGTC GAGCTGCAGA CGGCACATCG TGGAGCCGCC 
GGCCACCCGG CCACCTGGAT CGAGCAGTGT ACCTGTCCGG AGGGTTACCT GGGCCAGTTC 
TGTGAGTCCT GTGCTCCAGG CTATCGCCAC AGTCCCGCTC GCGGCGGTCC CTTCATGCCC 
TGCATCCCCT GCGATTGCCA TGGTCATGCG GACATCTGTG ACTCGGAGAC GGGCAGGTGC 
ATTTGCCAGC ACAACACCCA CGGCGATAAC TGCGATCAGT GTGCCAAGGG ATTCTACGGA 
AATGCCCTGG GCGGAACTCC CAACGACTGC AAGCGTTGCC CCTGTCCCAA TGATGGTGCC 
TGCCTGCAGA TCAACGAAGA TACGGTCATC TGTACGGAGT GCCCGAAGGG TTACTTTGGT 
TCCCGTTGCG AGCAGTGTAG CGATGGCTTC TTCGGAGATC CCACGGGTCT CCTGGGTGAG 
GTGCAGACTT GTAAGAGCTG TGACTGCAAT GGCAACGTGG ATCCCAATGC CGTGGGCAAT 
TGCAACCGGA CTACGGGCGA GTGCTTAAAG TGTATCCACA ATACAGCCGG AGAGCACTGT 
GATCAGTGTT TGTCCGGACA CTTTGGTGAT CCTCTGGCCT TGCCTCATGG ACGCTGTGAT 
CGCTGCAGTT GCTACGAGGC TGGAACCGAG CAGGATGAAC AGAGTATCAC GCGATGTGAC 
CAAGTCACTG GTCAGTGCCA GTGCAAGCCG AATGTAATTG GAAGGGATTG CGGAGAGTGC 
CAGCCGGGCT ATTTCAACAT CCGATCGGGC AATGGCTGCG AGAACTGCCT GTGCGATCCG 
GTGGGCAGCT ACAACTCCAC CTGCGATCGC TACTCTGGCC AGTGTCACTG CCGACCAGGT 
GTAATGGGTC AGCGGTGCGA TCAGTGCGAG AACTATTTCT ACGGATTCTC CAGCGAGGGC 
TGCAAGCCAT GCGAATGCGA CGAGAGCGGC TCAAAGGGAT TCCAGTGCGA CCAGAATGGT 
CAGTGTCCTT GCAATGATAA CGTGGAAGGA CGTCGTTGCG ATCGCTGCAA GGAGAACAAG 
TACGACAGGC ATCGGGGTTG CATCGATTGC CCCGATTGCT ATAACCTCGT CCAAGATGCC 
GCCGATTTGC ATCGTGCCAA GTTGTTCAAT CTCAGTCAAA CGCTGGACGA GATTGCTCGC 
ACGCCGGTGA CCAATGACGA TGAGTTCGAG GCCAAGTTGA AGGCGGTGCA GGAGAAGGTG 
GCTGTCCTGG CCCAGGATGC ACGCGATAAT TCCGGTGATG GTGGTCAAAC ATACGCAGAG 
GTCATCGATG ATCTTCACAA GCACCTGGAC AGCGTGAGAG AGCATCTGGT GAGCGCGGAT 
AAGTTCCAGG CAGATGCCAA TGGGGAGATC GACAGGGCGC GCCAGAACTA TACCATTCTG 
GACCAGATCA CCGAGAACGC CAAGAAGGAA CTGCAGCAGG CCCTCGACCT CCTGAATGAC 
GAAGGTGCTC AGGCTTTGGC CCGGGCCAAG GAGAAGTCTG TGGAATTTGG ACAGCAATCG 
GAACAGATCT CGGACATCTC GCGGGAGGCT CGTGCCCTGG CCGACAAACT GGAGTCGGAG 
GCCCAGTTCG ATTTGAAGAA CGCCAAGGAT GCCAAGGATG CGGTGGAGAA GGCCCATCAG 
CTGGCCAAGA GTGCCATCGA TTTGCAGCTA AAGATCGGCA CCGAGCTCCG TTCGGAGGTT 
GGTCTGGAGC TGAGTCATGT GAAACAATCC CTGGGCACCG TAGTGCAGAC CTCCAAGGAG 
GCTCTTCGCA AAGCCAACGA GGTCTACGAT ACAGCATTGA CCCTGCTCAA TGATGTCAAT 
CGTCAGACGC AACCGGAAAT CGATATCAGT CAGTTGAAGA AGGATGCAGT GGCCGCCAAT 
GAACGAGCGG ATGAGCTACT CAAGCAGATA ACTGAATTGT CCAACAGCAA CGGCGAACTC 
TTTGCGGATT TCGAAACGGA ACAGGAACTG ACGGAGGCGC TGCTTAAGAG AGCTGAACAG 
CAGCAACTGG AGGACATCGA GCTGCTAGAG CGAGCCAAGG CTGCCCACGA CAAGGCTACC 
AAGGCAGTGG AACAGGGCGA CAATACCCTA AAGGAGGCCA ACAATACCTA CGAGAAGTTG 
GCCGGCTTCC AGTCGGATGT CCAGCGCTCT TCTGAGAGTG CGGAGAAAGC CCTGCAGACG 
GTGCCCAACA TCGAGAAGGA GATTCAGAAT GCGGAAAGCC TGATCAGCCA GGCGGAGGAG 
GCTCTCGATG GAGCCAATAA GAATGCCAAC GAGGCCAAGA AGAATGCCCA GGAGGCGCAG 
CTGAAGTATG CCGAACAGGC TTCCAAGGAT GCTGAGCTGA TCCGCCGCAA AGCCAATGAG 
ACCAAGGTGG CTGCCCGCAA TCTGCGCGAA GAGGCCGACC AACTGAATCA TCGCGTGAAA 
CTCACCGAAA TGGACATCTT CAAGCTGGAG GAGAGCTCGA CCAAGGACGA CAATCTGGTG 
GACGACGCCA AGCGAAAGGT GGGTCAGGCC AAGGCCGATA CCCAGGAGGC CCAGAAGCAG 
ATCGAGAAGG CCAATGCCGA CCTGACGGCC ATCAAGGATG AGCTGGAGAA TCTGAAGGAC 
ATCAACACGG GCGATTTGGA TAGATTGGAG AATCGTTTGG CCACTGTGGA GGGTGAGATC 
AATCGCGTCA ACCTGACGGG ACGCATCGAA AAGTACCGGG AACAGCGCAC CATTCAGAAG 
AATCTGATCG ACAAGTACGA TGCCGAGCTG AGGGAGCTCA AGGATGAAGT CCAGAATATT 
GGACTTATTT CCAAGGCTCT CCCCGATAGC TGCTTCAGTC GCAATCGCCT GGAACCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524006.1
CDS	253..5172
Translation	MKRSRWSHSGSSTARLLLIGVLFASCSTAILGAQRPPINSAGGHELRGTTFMPALECYDPYGRPQKCLPEFINAAYQLQIESTNTCGEQNDNHFCIQTMNQNHKNCEFCKYNDHNPSFLTDLHDPQSPTWWQSETMFEGIQHPNYVNLTLHLGKSYDITYVRILFRSPRPESFTIYKRTSESGPWIPYQFYSATCRDTYSLPDSRAIRKGEGEAHALCTSEYSDISPLRDGEIAFSTLEGRPSGINFERSGELQEWVTATDIRITLDRLNTFGDELFGDSQVLKSYFYAISDIAVGARCKCNGHASKCVPSTGMHGERTLVCECRHNTDGPDCDRCLPLYNDLKWKRSTSTEVNECKACNCNGLADKCFFDANLFNRTGHGGHCLDCRENRDGPNCERCKENFYMRDDGYCVNCACDPVGSRSLQCNSHGKCQCKPGVTGDKCDRCDNNYYQFGPHGCQQCGCDSGGSHQNTPACDTETGICFCKENVEGRRCNECKPGFFNLDKNNRFGCTPCFCYGHTSECMTAPGYSIVSVTSNFNKFKERWTAADLNQREVDIKYNQYSRSIGTTAQGNEHVYFQAPDRFLGDQRASYNRDLKFKLQLVGQVANTGVSDVILEGAGSRISLPIFAQGNGIPDQGVKEYTFRLHEHHDYQWQPSQSARGFLSILSNLTAIKIRATYSVQGEAILDDVELQTAHRGAAGHPATWIEQCTCPEGYLGQFCESCAPGYRHSPARGGPFMPCIPCDCHGHADICDSETGRCICQHNTHGDNCDQCAKGFYGNALGGTPNDCKRCPCPNDGACLQINEDTVICTECPKGYFGSRCEQCSDGFFGDPTGLLGEVQTCKSCDCNGNVDPNAVGNCNRTTGECLKCIHNTAGEHCDQCLSGHFGDPLALPHGRCDRCSCYEAGTEQDEQSITRCDQVTGQCQCKPNVIGRDCGECQPGYFNIRSGNGCENCLCDPVGSYNSTCDRYSGQCHCRPGVMGQRCDQCENYFYGFSSEGCKPCECDESGSKGFQCDQNGQCPCNDNVEGRRCDRCKENKYDRHRGCIDCPDCYNLVQDAADLHRAKLFNLSQTLDEIARTPVTNDDEFEAKLKAVQEKVAVLAQDARDNSGDGGQTYAEVIDDLHKHLDSVREHLVSADKFQADANGEIDRARQNYTILDQITENAKKELQQALDLLNDEGAQALARAKEKSVEFGQQSEQISDISREARALADKLESEAQFDLKNAKDAKDAVEKAHQLAKSAIDLQLKIGTELRSEVGLELSHVKQSLGTVVQTSKEALRKANEVYDTALTLLNDVNRQTQPEIDISQLKKDAVAANERADELLKQITELSNSNGELFADFETEQELTEALLKRAEQQQLEDIELLERAKAAHDKATKAVEQGDNTLKEANNTYEKLAGFQSDVQRSSESAEKALQTVPNIEKEIQNAESLISQAEEALDGANKNANEAKKNAQEAQLKYAEQASKDAELIRRKANETKVAARNLREEADQLNHRVKLTEMDIFKLEESSTKDDNLVDDAKRKVGQAKADTQEAQKQIEKANADLTAIKDELENLKDINTGDLDRLENRLATVEGEINRVNLTGRIEKYREQRTIQKNLIDKYDAELRELKDEVQNIGLISKALPDSCFSRNRLEP

Target ORF information:

RefSeq Version	NM_079282.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster laminin B2, transcript variant A (LanB2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079282.2

ORF Insert Sequence:

ATGAAGCGCA GCCGGTGGAG CCACAGTGGC TCCTCCACGG CTCGACTCCT GCTGATCGGA 
GTATTGTTCG CCAGCTGTTC CACCGCCATC CTGGGTGCCC AGCGTCCCCC CATCAACTCC 
GCCGGAGGTC ACGAACTGCG CGGAACCACC TTCATGCCGG CCCTGGAGTG CTACGATCCA 
TACGGCAGGC CACAGAAATG TCTGCCAGAA TTTATCAATG CTGCCTATCA ACTGCAAATT 
GAGTCAACTA ATACCTGTGG TGAGCAGAAT GACAACCACT TCTGCATACA AACCATGAAC 
CAAAATCACA AAAACTGCGA ATTTTGCAAA TACAATGATC ATAATCCATC CTTCTTGACG 
GATTTGCATG ATCCGCAAAG TCCAACGTGG TGGCAATCGG AGACCATGTT CGAGGGCATT 
CAGCACCCGA ACTATGTGAA TCTGACTTTG CACCTTGGAA AATCCTATGA CATCACCTAC 
GTGCGCATTC TCTTCCGCTC ACCAAGACCC GAATCCTTTA CGATTTACAA GAGGACCTCG 
GAGAGTGGAC CCTGGATTCC CTACCAGTTC TACAGTGCCA CCTGTCGCGA CACCTACTCC 
CTGCCAGATT CGCGAGCCAT TCGCAAGGGT GAGGGCGAGG CCCATGCTCT GTGTACCAGC 
GAATACAGTG ATATCTCGCC GTTGAGGGAC GGTGAGATTG CCTTCTCCAC GCTGGAGGGT 
CGTCCCAGTG GCATCAATTT TGAGCGCAGC GGAGAACTGC AGGAGTGGGT CACGGCCACG 
GATATCCGTA TCACGCTGGA CCGACTGAAC ACCTTCGGTG ACGAACTCTT CGGTGATTCC 
CAGGTGCTCA AGTCGTACTT CTATGCCATC AGTGACATTG CCGTGGGTGC GCGTTGCAAG 
TGCAATGGAC ATGCCAGCAA GTGTGTACCG AGCACGGGAA TGCATGGCGA GAGGACTCTG 
GTCTGCGAGT GCCGGCACAA TACGGATGGA CCCGATTGCG ATCGCTGTCT GCCACTCTAC 
AACGACCTCA AGTGGAAGAG ATCCACCTCA ACGGAGGTCA ACGAGTGCAA AGCTTGCAAC 
TGCAATGGAT TGGCGGACAA GTGCTTCTTC GACGCGAATC TGTTCAATCG GACGGGTCAT 
GGAGGTCACT GCCTGGACTG CCGCGAGAAT CGCGATGGAC CCAACTGCGA ACGCTGCAAG 
GAGAACTTCT ATATGCGCGA CGATGGCTAC TGCGTCAACT GCGCCTGCGA TCCCGTTGGC 
TCCAGATCGC TGCAGTGCAA CAGCCACGGC AAGTGCCAGT GTAAGCCGGG TGTGACTGGC 
GACAAGTGCG ACCGCTGCGA TAACAACTAC TACCAATTCG GTCCCCATGG ATGCCAGCAG 
TGCGGATGCG ACAGTGGGGG ATCCCATCAG AATACACCCG CCTGCGATAC CGAGACTGGA 
ATCTGCTTCT GCAAGGAGAA TGTGGAGGGC AGACGCTGCA ATGAATGCAA GCCAGGCTTC 
TTCAATCTGG ACAAGAACAA TCGATTTGGT TGCACACCCT GCTTCTGCTA TGGGCACACC 
TCCGAGTGCA TGACTGCCCC AGGATACTCC ATTGTTTCGG TCACCTCCAA TTTTAACAAA 
TTCAAGGAAC GCTGGACGGC CGCCGATTTG AATCAACGCG AGGTGGACAT CAAGTACAAC 
CAGTATAGCC GAAGCATTGG AACCACCGCT CAGGGAAATG AGCACGTCTA CTTCCAGGCA 
CCGGATCGCT TCCTCGGCGA TCAGCGTGCC TCCTACAACA GGGATCTGAA ATTCAAGCTC 
CAGCTAGTTG GTCAGGTGGC CAATACCGGA GTGAGTGACG TGATCTTGGA GGGTGCTGGC 
AGCCGCATCT CCCTGCCCAT CTTCGCCCAG GGCAATGGAA TACCCGACCA GGGAGTTAAG 
GAGTATACCT TCCGTCTGCA TGAACATCAT GATTACCAGT GGCAACCAAG CCAGTCGGCT 
CGTGGATTCC TTTCGATTCT GTCCAATCTG ACGGCCATTA AGATCAGGGC CACGTACTCG 
GTGCAGGGTG AGGCCATCCT GGATGATGTC GAGCTGCAGA CGGCACATCG TGGAGCCGCC 
GGCCACCCGG CCACCTGGAT CGAGCAGTGT ACCTGTCCGG AGGGTTACCT GGGCCAGTTC 
TGTGAGTCCT GTGCTCCAGG CTATCGCCAC AGTCCCGCTC GCGGCGGTCC CTTCATGCCC 
TGCATCCCCT GCGATTGCCA TGGTCATGCG GACATCTGTG ACTCGGAGAC GGGCAGGTGC 
ATTTGCCAGC ACAACACCCA CGGCGATAAC TGCGATCAGT GTGCCAAGGG ATTCTACGGA 
AATGCCCTGG GCGGAACTCC CAACGACTGC AAGCGTTGCC CCTGTCCCAA TGATGGTGCC 
TGCCTGCAGA TCAACGAAGA TACGGTCATC TGTACGGAGT GCCCGAAGGG TTACTTTGGT 
TCCCGTTGCG AGCAGTGTAG CGATGGCTTC TTCGGAGATC CCACGGGTCT CCTGGGTGAG 
GTGCAGACTT GTAAGAGCTG TGACTGCAAT GGCAACGTGG ATCCCAATGC CGTGGGCAAT 
TGCAACCGGA CTACGGGCGA GTGCTTAAAG TGTATCCACA ATACAGCCGG AGAGCACTGT 
GATCAGTGTT TGTCCGGACA CTTTGGTGAT CCTCTGGCCT TGCCTCATGG ACGCTGTGAT 
CGCTGCAGTT GCTACGAGGC TGGAACCGAG CAGGATGAAC AGAGTATCAC GCGATGTGAC 
CAAGTCACTG GTCAGTGCCA GTGCAAGCCG AATGTAATTG GAAGGGATTG CGGAGAGTGC 
CAGCCGGGCT ATTTCAACAT CCGATCGGGC AATGGCTGCG AGAACTGCCT GTGCGATCCG 
GTGGGCAGCT ACAACTCCAC CTGCGATCGC TACTCTGGCC AGTGTCACTG CCGACCAGGT 
GTAATGGGTC AGCGGTGCGA TCAGTGCGAG AACTATTTCT ACGGATTCTC CAGCGAGGGC 
TGCAAGCCAT GCGAATGCGA CGAGAGCGGC TCAAAGGGAT TCCAGTGCGA CCAGAATGGT 
CAGTGTCCTT GCAATGATAA CGTGGAAGGA CGTCGTTGCG ATCGCTGCAA GGAGAACAAG 
TACGACAGGC ATCGGGGTTG CATCGATTGC CCCGATTGCT ATAACCTCGT CCAAGATGCC 
GCCGATTTGC ATCGTGCCAA GTTGTTCAAT CTCAGTCAAA CGCTGGACGA GATTGCTCGC 
ACGCCGGTGA CCAATGACGA TGAGTTCGAG GCCAAGTTGA AGGCGGTGCA GGAGAAGGTG 
GCTGTCCTGG CCCAGGATGC ACGCGATAAT TCCGGTGATG GTGGTCAAAC ATACGCAGAG 
GTCATCGATG ATCTTCACAA GCACCTGGAC AGCGTGAGAG AGCATCTGGT GAGCGCGGAT 
AAGTTCCAGG CAGATGCCAA TGGGGAGATC GACAGGGCGC GCCAGAACTA TACCATTCTG 
GACCAGATCA CCGAGAACGC CAAGAAGGAA CTGCAGCAGG CCCTCGACCT CCTGAATGAC 
GAAGGTGCTC AGGCTTTGGC CCGGGCCAAG GAGAAGTCTG TGGAATTTGG ACAGCAATCG 
GAACAGATCT CGGACATCTC GCGGGAGGCT CGTGCCCTGG CCGACAAACT GGAGTCGGAG 
GCCCAGTTCG ATTTGAAGAA CGCCAAGGAT GCCAAGGATG CGGTGGAGAA GGCCCATCAG 
CTGGCCAAGA GTGCCATCGA TTTGCAGCTA AAGATCGGCA CCGAGCTCCG TTCGGAGGTT 
GGTCTGGAGC TGAGTCATGT GAAACAATCC CTGGGCACCG TAGTGCAGAC CTCCAAGGAG 
GCTCTTCGCA AAGCCAACGA GGTCTACGAT ACAGCATTGA CCCTGCTCAA TGATGTCAAT 
CGTCAGACGC AACCGGAAAT CGATATCAGT CAGTTGAAGA AGGATGCAGT GGCCGCCAAT 
GAACGAGCGG ATGAGCTACT CAAGCAGATA ACTGAATTGT CCAACAGCAA CGGCGAACTC 
TTTGCGGATT TCGAAACGGA ACAGGAACTG ACGGAGGCGC TGCTTAAGAG AGCTGAACAG 
CAGCAACTGG AGGACATCGA GCTGCTAGAG CGAGCCAAGG CTGCCCACGA CAAGGCTACC 
AAGGCAGTGG AACAGGGCGA CAATACCCTA AAGGAGGCCA ACAATACCTA CGAGAAGTTG 
GCCGGCTTCC AGTCGGATGT CCAGCGCTCT TCTGAGAGTG CGGAGAAAGC CCTGCAGACG 
GTGCCCAACA TCGAGAAGGA GATTCAGAAT GCGGAAAGCC TGATCAGCCA GGCGGAGGAG 
GCTCTCGATG GAGCCAATAA GAATGCCAAC GAGGCCAAGA AGAATGCCCA GGAGGCGCAG 
CTGAAGTATG CCGAACAGGC TTCCAAGGAT GCTGAGCTGA TCCGCCGCAA AGCCAATGAG 
ACCAAGGTGG CTGCCCGCAA TCTGCGCGAA GAGGCCGACC AACTGAATCA TCGCGTGAAA 
CTCACCGAAA TGGACATCTT CAAGCTGGAG GAGAGCTCGA CCAAGGACGA CAATCTGGTG 
GACGACGCCA AGCGAAAGGT GGGTCAGGCC AAGGCCGATA CCCAGGAGGC CCAGAAGCAG 
ATCGAGAAGG CCAATGCCGA CCTGACGGCC ATCAAGGATG AGCTGGAGAA TCTGAAGGAC 
ATCAACACGG GCGATTTGGA TAGATTGGAG AATCGTTTGG CCACTGTGGA GGGTGAGATC 
AATCGCGTCA ACCTGACGGG ACGCATCGAA AAGTACCGGG AACAGCGCAC CATTCAGAAG 
AATCTGATCG ACAAGTACGA TGCCGAGCTG AGGGAGCTCA AGGATGAAGT CCAGAATATT 
GGACTTATTT CCAAGGCTCT CCCCGATAGC TGCTTCAGTC GCAATCGCCT GGAACCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LanB2 ( NM_001300080.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287009.1 Drosophila melanogaster laminin B2, transcript variant B (LanB2), mRNA.

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CloneID	OFa18066
Clone ID Related Accession (Same CDS sequence)	NM_079282.2 , NM_001300080.1
Accession Version	NM_001300080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	laminin B2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCGCA GCCGGTGGAG CCACAGTGGC TCCTCCACGG CTCGACTCCT GCTGATCGGA 
GTATTGTTCG CCAGCTGTTC CACCGCCATC CTGGGTGCCC AGCGTCCCCC CATCAACTCC 
GCCGGAGGTC ACGAACTGCG CGGAACCACC TTCATGCCGG CCCTGGAGTG CTACGATCCA 
TACGGCAGGC CACAGAAATG TCTGCCAGAA TTTATCAATG CTGCCTATCA ACTGCAAATT 
GAGTCAACTA ATACCTGTGG TGAGCAGAAT GACAACCACT TCTGCATACA AACCATGAAC 
CAAAATCACA AAAACTGCGA ATTTTGCAAA TACAATGATC ATAATCCATC CTTCTTGACG 
GATTTGCATG ATCCGCAAAG TCCAACGTGG TGGCAATCGG AGACCATGTT CGAGGGCATT 
CAGCACCCGA ACTATGTGAA TCTGACTTTG CACCTTGGAA AATCCTATGA CATCACCTAC 
GTGCGCATTC TCTTCCGCTC ACCAAGACCC GAATCCTTTA CGATTTACAA GAGGACCTCG 
GAGAGTGGAC CCTGGATTCC CTACCAGTTC TACAGTGCCA CCTGTCGCGA CACCTACTCC 
CTGCCAGATT CGCGAGCCAT TCGCAAGGGT GAGGGCGAGG CCCATGCTCT GTGTACCAGC 
GAATACAGTG ATATCTCGCC GTTGAGGGAC GGTGAGATTG CCTTCTCCAC GCTGGAGGGT 
CGTCCCAGTG GCATCAATTT TGAGCGCAGC GGAGAACTGC AGGAGTGGGT CACGGCCACG 
GATATCCGTA TCACGCTGGA CCGACTGAAC ACCTTCGGTG ACGAACTCTT CGGTGATTCC 
CAGGTGCTCA AGTCGTACTT CTATGCCATC AGTGACATTG CCGTGGGTGC GCGTTGCAAG 
TGCAATGGAC ATGCCAGCAA GTGTGTACCG AGCACGGGAA TGCATGGCGA GAGGACTCTG 
GTCTGCGAGT GCCGGCACAA TACGGATGGA CCCGATTGCG ATCGCTGTCT GCCACTCTAC 
AACGACCTCA AGTGGAAGAG ATCCACCTCA ACGGAGGTCA ACGAGTGCAA AGCTTGCAAC 
TGCAATGGAT TGGCGGACAA GTGCTTCTTC GACGCGAATC TGTTCAATCG GACGGGTCAT 
GGAGGTCACT GCCTGGACTG CCGCGAGAAT CGCGATGGAC CCAACTGCGA ACGCTGCAAG 
GAGAACTTCT ATATGCGCGA CGATGGCTAC TGCGTCAACT GCGCCTGCGA TCCCGTTGGC 
TCCAGATCGC TGCAGTGCAA CAGCCACGGC AAGTGCCAGT GTAAGCCGGG TGTGACTGGC 
GACAAGTGCG ACCGCTGCGA TAACAACTAC TACCAATTCG GTCCCCATGG ATGCCAGCAG 
TGCGGATGCG ACAGTGGGGG ATCCCATCAG AATACACCCG CCTGCGATAC CGAGACTGGA 
ATCTGCTTCT GCAAGGAGAA TGTGGAGGGC AGACGCTGCA ATGAATGCAA GCCAGGCTTC 
TTCAATCTGG ACAAGAACAA TCGATTTGGT TGCACACCCT GCTTCTGCTA TGGGCACACC 
TCCGAGTGCA TGACTGCCCC AGGATACTCC ATTGTTTCGG TCACCTCCAA TTTTAACAAA 
TTCAAGGAAC GCTGGACGGC CGCCGATTTG AATCAACGCG AGGTGGACAT CAAGTACAAC 
CAGTATAGCC GAAGCATTGG AACCACCGCT CAGGGAAATG AGCACGTCTA CTTCCAGGCA 
CCGGATCGCT TCCTCGGCGA TCAGCGTGCC TCCTACAACA GGGATCTGAA ATTCAAGCTC 
CAGCTAGTTG GTCAGGTGGC CAATACCGGA GTGAGTGACG TGATCTTGGA GGGTGCTGGC 
AGCCGCATCT CCCTGCCCAT CTTCGCCCAG GGCAATGGAA TACCCGACCA GGGAGTTAAG 
GAGTATACCT TCCGTCTGCA TGAACATCAT GATTACCAGT GGCAACCAAG CCAGTCGGCT 
CGTGGATTCC TTTCGATTCT GTCCAATCTG ACGGCCATTA AGATCAGGGC CACGTACTCG 
GTGCAGGGTG AGGCCATCCT GGATGATGTC GAGCTGCAGA CGGCACATCG TGGAGCCGCC 
GGCCACCCGG CCACCTGGAT CGAGCAGTGT ACCTGTCCGG AGGGTTACCT GGGCCAGTTC 
TGTGAGTCCT GTGCTCCAGG CTATCGCCAC AGTCCCGCTC GCGGCGGTCC CTTCATGCCC 
TGCATCCCCT GCGATTGCCA TGGTCATGCG GACATCTGTG ACTCGGAGAC GGGCAGGTGC 
ATTTGCCAGC ACAACACCCA CGGCGATAAC TGCGATCAGT GTGCCAAGGG ATTCTACGGA 
AATGCCCTGG GCGGAACTCC CAACGACTGC AAGCGTTGCC CCTGTCCCAA TGATGGTGCC 
TGCCTGCAGA TCAACGAAGA TACGGTCATC TGTACGGAGT GCCCGAAGGG TTACTTTGGT 
TCCCGTTGCG AGCAGTGTAG CGATGGCTTC TTCGGAGATC CCACGGGTCT CCTGGGTGAG 
GTGCAGACTT GTAAGAGCTG TGACTGCAAT GGCAACGTGG ATCCCAATGC CGTGGGCAAT 
TGCAACCGGA CTACGGGCGA GTGCTTAAAG TGTATCCACA ATACAGCCGG AGAGCACTGT 
GATCAGTGTT TGTCCGGACA CTTTGGTGAT CCTCTGGCCT TGCCTCATGG ACGCTGTGAT 
CGCTGCAGTT GCTACGAGGC TGGAACCGAG CAGGATGAAC AGAGTATCAC GCGATGTGAC 
CAAGTCACTG GTCAGTGCCA GTGCAAGCCG AATGTAATTG GAAGGGATTG CGGAGAGTGC 
CAGCCGGGCT ATTTCAACAT CCGATCGGGC AATGGCTGCG AGAACTGCCT GTGCGATCCG 
GTGGGCAGCT ACAACTCCAC CTGCGATCGC TACTCTGGCC AGTGTCACTG CCGACCAGGT 
GTAATGGGTC AGCGGTGCGA TCAGTGCGAG AACTATTTCT ACGGATTCTC CAGCGAGGGC 
TGCAAGCCAT GCGAATGCGA CGAGAGCGGC TCAAAGGGAT TCCAGTGCGA CCAGAATGGT 
CAGTGTCCTT GCAATGATAA CGTGGAAGGA CGTCGTTGCG ATCGCTGCAA GGAGAACAAG 
TACGACAGGC ATCGGGGTTG CATCGATTGC CCCGATTGCT ATAACCTCGT CCAAGATGCC 
GCCGATTTGC ATCGTGCCAA GTTGTTCAAT CTCAGTCAAA CGCTGGACGA GATTGCTCGC 
ACGCCGGTGA CCAATGACGA TGAGTTCGAG GCCAAGTTGA AGGCGGTGCA GGAGAAGGTG 
GCTGTCCTGG CCCAGGATGC ACGCGATAAT TCCGGTGATG GTGGTCAAAC ATACGCAGAG 
GTCATCGATG ATCTTCACAA GCACCTGGAC AGCGTGAGAG AGCATCTGGT GAGCGCGGAT 
AAGTTCCAGG CAGATGCCAA TGGGGAGATC GACAGGGCGC GCCAGAACTA TACCATTCTG 
GACCAGATCA CCGAGAACGC CAAGAAGGAA CTGCAGCAGG CCCTCGACCT CCTGAATGAC 
GAAGGTGCTC AGGCTTTGGC CCGGGCCAAG GAGAAGTCTG TGGAATTTGG ACAGCAATCG 
GAACAGATCT CGGACATCTC GCGGGAGGCT CGTGCCCTGG CCGACAAACT GGAGTCGGAG 
GCCCAGTTCG ATTTGAAGAA CGCCAAGGAT GCCAAGGATG CGGTGGAGAA GGCCCATCAG 
CTGGCCAAGA GTGCCATCGA TTTGCAGCTA AAGATCGGCA CCGAGCTCCG TTCGGAGGTT 
GGTCTGGAGC TGAGTCATGT GAAACAATCC CTGGGCACCG TAGTGCAGAC CTCCAAGGAG 
GCTCTTCGCA AAGCCAACGA GGTCTACGAT ACAGCATTGA CCCTGCTCAA TGATGTCAAT 
CGTCAGACGC AACCGGAAAT CGATATCAGT CAGTTGAAGA AGGATGCAGT GGCCGCCAAT 
GAACGAGCGG ATGAGCTACT CAAGCAGATA ACTGAATTGT CCAACAGCAA CGGCGAACTC 
TTTGCGGATT TCGAAACGGA ACAGGAACTG ACGGAGGCGC TGCTTAAGAG AGCTGAACAG 
CAGCAACTGG AGGACATCGA GCTGCTAGAG CGAGCCAAGG CTGCCCACGA CAAGGCTACC 
AAGGCAGTGG AACAGGGCGA CAATACCCTA AAGGAGGCCA ACAATACCTA CGAGAAGTTG 
GCCGGCTTCC AGTCGGATGT CCAGCGCTCT TCTGAGAGTG CGGAGAAAGC CCTGCAGACG 
GTGCCCAACA TCGAGAAGGA GATTCAGAAT GCGGAAAGCC TGATCAGCCA GGCGGAGGAG 
GCTCTCGATG GAGCCAATAA GAATGCCAAC GAGGCCAAGA AGAATGCCCA GGAGGCGCAG 
CTGAAGTATG CCGAACAGGC TTCCAAGGAT GCTGAGCTGA TCCGCCGCAA AGCCAATGAG 
ACCAAGGTGG CTGCCCGCAA TCTGCGCGAA GAGGCCGACC AACTGAATCA TCGCGTGAAA 
CTCACCGAAA TGGACATCTT CAAGCTGGAG GAGAGCTCGA CCAAGGACGA CAATCTGGTG 
GACGACGCCA AGCGAAAGGT GGGTCAGGCC AAGGCCGATA CCCAGGAGGC CCAGAAGCAG 
ATCGAGAAGG CCAATGCCGA CCTGACGGCC ATCAAGGATG AGCTGGAGAA TCTGAAGGAC 
ATCAACACGG GCGATTTGGA TAGATTGGAG AATCGTTTGG CCACTGTGGA GGGTGAGATC 
AATCGCGTCA ACCTGACGGG ACGCATCGAA AAGTACCGGG AACAGCGCAC CATTCAGAAG 
AATCTGATCG ACAAGTACGA TGCCGAGCTG AGGGAGCTCA AGGATGAAGT CCAGAATATT 
GGACTTATTT CCAAGGCTCT CCCCGATAGC TGCTTCAGTC GCAATCGCCT GGAACCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287009.1
CDS	348..5267
Translation	MKRSRWSHSGSSTARLLLIGVLFASCSTAILGAQRPPINSAGGHELRGTTFMPALECYDPYGRPQKCLPEFINAAYQLQIESTNTCGEQNDNHFCIQTMNQNHKNCEFCKYNDHNPSFLTDLHDPQSPTWWQSETMFEGIQHPNYVNLTLHLGKSYDITYVRILFRSPRPESFTIYKRTSESGPWIPYQFYSATCRDTYSLPDSRAIRKGEGEAHALCTSEYSDISPLRDGEIAFSTLEGRPSGINFERSGELQEWVTATDIRITLDRLNTFGDELFGDSQVLKSYFYAISDIAVGARCKCNGHASKCVPSTGMHGERTLVCECRHNTDGPDCDRCLPLYNDLKWKRSTSTEVNECKACNCNGLADKCFFDANLFNRTGHGGHCLDCRENRDGPNCERCKENFYMRDDGYCVNCACDPVGSRSLQCNSHGKCQCKPGVTGDKCDRCDNNYYQFGPHGCQQCGCDSGGSHQNTPACDTETGICFCKENVEGRRCNECKPGFFNLDKNNRFGCTPCFCYGHTSECMTAPGYSIVSVTSNFNKFKERWTAADLNQREVDIKYNQYSRSIGTTAQGNEHVYFQAPDRFLGDQRASYNRDLKFKLQLVGQVANTGVSDVILEGAGSRISLPIFAQGNGIPDQGVKEYTFRLHEHHDYQWQPSQSARGFLSILSNLTAIKIRATYSVQGEAILDDVELQTAHRGAAGHPATWIEQCTCPEGYLGQFCESCAPGYRHSPARGGPFMPCIPCDCHGHADICDSETGRCICQHNTHGDNCDQCAKGFYGNALGGTPNDCKRCPCPNDGACLQINEDTVICTECPKGYFGSRCEQCSDGFFGDPTGLLGEVQTCKSCDCNGNVDPNAVGNCNRTTGECLKCIHNTAGEHCDQCLSGHFGDPLALPHGRCDRCSCYEAGTEQDEQSITRCDQVTGQCQCKPNVIGRDCGECQPGYFNIRSGNGCENCLCDPVGSYNSTCDRYSGQCHCRPGVMGQRCDQCENYFYGFSSEGCKPCECDESGSKGFQCDQNGQCPCNDNVEGRRCDRCKENKYDRHRGCIDCPDCYNLVQDAADLHRAKLFNLSQTLDEIARTPVTNDDEFEAKLKAVQEKVAVLAQDARDNSGDGGQTYAEVIDDLHKHLDSVREHLVSADKFQADANGEIDRARQNYTILDQITENAKKELQQALDLLNDEGAQALARAKEKSVEFGQQSEQISDISREARALADKLESEAQFDLKNAKDAKDAVEKAHQLAKSAIDLQLKIGTELRSEVGLELSHVKQSLGTVVQTSKEALRKANEVYDTALTLLNDVNRQTQPEIDISQLKKDAVAANERADELLKQITELSNSNGELFADFETEQELTEALLKRAEQQQLEDIELLERAKAAHDKATKAVEQGDNTLKEANNTYEKLAGFQSDVQRSSESAEKALQTVPNIEKEIQNAESLISQAEEALDGANKNANEAKKNAQEAQLKYAEQASKDAELIRRKANETKVAARNLREEADQLNHRVKLTEMDIFKLEESSTKDDNLVDDAKRKVGQAKADTQEAQKQIEKANADLTAIKDELENLKDINTGDLDRLENRLATVEGEINRVNLTGRIEKYREQRTIQKNLIDKYDAELRELKDEVQNIGLISKALPDSCFSRNRLEP

Target ORF information:

RefSeq Version	NM_001300080.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster laminin B2, transcript variant B (LanB2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300080.1

ORF Insert Sequence:

ATGAAGCGCA GCCGGTGGAG CCACAGTGGC TCCTCCACGG CTCGACTCCT GCTGATCGGA 
GTATTGTTCG CCAGCTGTTC CACCGCCATC CTGGGTGCCC AGCGTCCCCC CATCAACTCC 
GCCGGAGGTC ACGAACTGCG CGGAACCACC TTCATGCCGG CCCTGGAGTG CTACGATCCA 
TACGGCAGGC CACAGAAATG TCTGCCAGAA TTTATCAATG CTGCCTATCA ACTGCAAATT 
GAGTCAACTA ATACCTGTGG TGAGCAGAAT GACAACCACT TCTGCATACA AACCATGAAC 
CAAAATCACA AAAACTGCGA ATTTTGCAAA TACAATGATC ATAATCCATC CTTCTTGACG 
GATTTGCATG ATCCGCAAAG TCCAACGTGG TGGCAATCGG AGACCATGTT CGAGGGCATT 
CAGCACCCGA ACTATGTGAA TCTGACTTTG CACCTTGGAA AATCCTATGA CATCACCTAC 
GTGCGCATTC TCTTCCGCTC ACCAAGACCC GAATCCTTTA CGATTTACAA GAGGACCTCG 
GAGAGTGGAC CCTGGATTCC CTACCAGTTC TACAGTGCCA CCTGTCGCGA CACCTACTCC 
CTGCCAGATT CGCGAGCCAT TCGCAAGGGT GAGGGCGAGG CCCATGCTCT GTGTACCAGC 
GAATACAGTG ATATCTCGCC GTTGAGGGAC GGTGAGATTG CCTTCTCCAC GCTGGAGGGT 
CGTCCCAGTG GCATCAATTT TGAGCGCAGC GGAGAACTGC AGGAGTGGGT CACGGCCACG 
GATATCCGTA TCACGCTGGA CCGACTGAAC ACCTTCGGTG ACGAACTCTT CGGTGATTCC 
CAGGTGCTCA AGTCGTACTT CTATGCCATC AGTGACATTG CCGTGGGTGC GCGTTGCAAG 
TGCAATGGAC ATGCCAGCAA GTGTGTACCG AGCACGGGAA TGCATGGCGA GAGGACTCTG 
GTCTGCGAGT GCCGGCACAA TACGGATGGA CCCGATTGCG ATCGCTGTCT GCCACTCTAC 
AACGACCTCA AGTGGAAGAG ATCCACCTCA ACGGAGGTCA ACGAGTGCAA AGCTTGCAAC 
TGCAATGGAT TGGCGGACAA GTGCTTCTTC GACGCGAATC TGTTCAATCG GACGGGTCAT 
GGAGGTCACT GCCTGGACTG CCGCGAGAAT CGCGATGGAC CCAACTGCGA ACGCTGCAAG 
GAGAACTTCT ATATGCGCGA CGATGGCTAC TGCGTCAACT GCGCCTGCGA TCCCGTTGGC 
TCCAGATCGC TGCAGTGCAA CAGCCACGGC AAGTGCCAGT GTAAGCCGGG TGTGACTGGC 
GACAAGTGCG ACCGCTGCGA TAACAACTAC TACCAATTCG GTCCCCATGG ATGCCAGCAG 
TGCGGATGCG ACAGTGGGGG ATCCCATCAG AATACACCCG CCTGCGATAC CGAGACTGGA 
ATCTGCTTCT GCAAGGAGAA TGTGGAGGGC AGACGCTGCA ATGAATGCAA GCCAGGCTTC 
TTCAATCTGG ACAAGAACAA TCGATTTGGT TGCACACCCT GCTTCTGCTA TGGGCACACC 
TCCGAGTGCA TGACTGCCCC AGGATACTCC ATTGTTTCGG TCACCTCCAA TTTTAACAAA 
TTCAAGGAAC GCTGGACGGC CGCCGATTTG AATCAACGCG AGGTGGACAT CAAGTACAAC 
CAGTATAGCC GAAGCATTGG AACCACCGCT CAGGGAAATG AGCACGTCTA CTTCCAGGCA 
CCGGATCGCT TCCTCGGCGA TCAGCGTGCC TCCTACAACA GGGATCTGAA ATTCAAGCTC 
CAGCTAGTTG GTCAGGTGGC CAATACCGGA GTGAGTGACG TGATCTTGGA GGGTGCTGGC 
AGCCGCATCT CCCTGCCCAT CTTCGCCCAG GGCAATGGAA TACCCGACCA GGGAGTTAAG 
GAGTATACCT TCCGTCTGCA TGAACATCAT GATTACCAGT GGCAACCAAG CCAGTCGGCT 
CGTGGATTCC TTTCGATTCT GTCCAATCTG ACGGCCATTA AGATCAGGGC CACGTACTCG 
GTGCAGGGTG AGGCCATCCT GGATGATGTC GAGCTGCAGA CGGCACATCG TGGAGCCGCC 
GGCCACCCGG CCACCTGGAT CGAGCAGTGT ACCTGTCCGG AGGGTTACCT GGGCCAGTTC 
TGTGAGTCCT GTGCTCCAGG CTATCGCCAC AGTCCCGCTC GCGGCGGTCC CTTCATGCCC 
TGCATCCCCT GCGATTGCCA TGGTCATGCG GACATCTGTG ACTCGGAGAC GGGCAGGTGC 
ATTTGCCAGC ACAACACCCA CGGCGATAAC TGCGATCAGT GTGCCAAGGG ATTCTACGGA 
AATGCCCTGG GCGGAACTCC CAACGACTGC AAGCGTTGCC CCTGTCCCAA TGATGGTGCC 
TGCCTGCAGA TCAACGAAGA TACGGTCATC TGTACGGAGT GCCCGAAGGG TTACTTTGGT 
TCCCGTTGCG AGCAGTGTAG CGATGGCTTC TTCGGAGATC CCACGGGTCT CCTGGGTGAG 
GTGCAGACTT GTAAGAGCTG TGACTGCAAT GGCAACGTGG ATCCCAATGC CGTGGGCAAT 
TGCAACCGGA CTACGGGCGA GTGCTTAAAG TGTATCCACA ATACAGCCGG AGAGCACTGT 
GATCAGTGTT TGTCCGGACA CTTTGGTGAT CCTCTGGCCT TGCCTCATGG ACGCTGTGAT 
CGCTGCAGTT GCTACGAGGC TGGAACCGAG CAGGATGAAC AGAGTATCAC GCGATGTGAC 
CAAGTCACTG GTCAGTGCCA GTGCAAGCCG AATGTAATTG GAAGGGATTG CGGAGAGTGC 
CAGCCGGGCT ATTTCAACAT CCGATCGGGC AATGGCTGCG AGAACTGCCT GTGCGATCCG 
GTGGGCAGCT ACAACTCCAC CTGCGATCGC TACTCTGGCC AGTGTCACTG CCGACCAGGT 
GTAATGGGTC AGCGGTGCGA TCAGTGCGAG AACTATTTCT ACGGATTCTC CAGCGAGGGC 
TGCAAGCCAT GCGAATGCGA CGAGAGCGGC TCAAAGGGAT TCCAGTGCGA CCAGAATGGT 
CAGTGTCCTT GCAATGATAA CGTGGAAGGA CGTCGTTGCG ATCGCTGCAA GGAGAACAAG 
TACGACAGGC ATCGGGGTTG CATCGATTGC CCCGATTGCT ATAACCTCGT CCAAGATGCC 
GCCGATTTGC ATCGTGCCAA GTTGTTCAAT CTCAGTCAAA CGCTGGACGA GATTGCTCGC 
ACGCCGGTGA CCAATGACGA TGAGTTCGAG GCCAAGTTGA AGGCGGTGCA GGAGAAGGTG 
GCTGTCCTGG CCCAGGATGC ACGCGATAAT TCCGGTGATG GTGGTCAAAC ATACGCAGAG 
GTCATCGATG ATCTTCACAA GCACCTGGAC AGCGTGAGAG AGCATCTGGT GAGCGCGGAT 
AAGTTCCAGG CAGATGCCAA TGGGGAGATC GACAGGGCGC GCCAGAACTA TACCATTCTG 
GACCAGATCA CCGAGAACGC CAAGAAGGAA CTGCAGCAGG CCCTCGACCT CCTGAATGAC 
GAAGGTGCTC AGGCTTTGGC CCGGGCCAAG GAGAAGTCTG TGGAATTTGG ACAGCAATCG 
GAACAGATCT CGGACATCTC GCGGGAGGCT CGTGCCCTGG CCGACAAACT GGAGTCGGAG 
GCCCAGTTCG ATTTGAAGAA CGCCAAGGAT GCCAAGGATG CGGTGGAGAA GGCCCATCAG 
CTGGCCAAGA GTGCCATCGA TTTGCAGCTA AAGATCGGCA CCGAGCTCCG TTCGGAGGTT 
GGTCTGGAGC TGAGTCATGT GAAACAATCC CTGGGCACCG TAGTGCAGAC CTCCAAGGAG 
GCTCTTCGCA AAGCCAACGA GGTCTACGAT ACAGCATTGA CCCTGCTCAA TGATGTCAAT 
CGTCAGACGC AACCGGAAAT CGATATCAGT CAGTTGAAGA AGGATGCAGT GGCCGCCAAT 
GAACGAGCGG ATGAGCTACT CAAGCAGATA ACTGAATTGT CCAACAGCAA CGGCGAACTC 
TTTGCGGATT TCGAAACGGA ACAGGAACTG ACGGAGGCGC TGCTTAAGAG AGCTGAACAG 
CAGCAACTGG AGGACATCGA GCTGCTAGAG CGAGCCAAGG CTGCCCACGA CAAGGCTACC 
AAGGCAGTGG AACAGGGCGA CAATACCCTA AAGGAGGCCA ACAATACCTA CGAGAAGTTG 
GCCGGCTTCC AGTCGGATGT CCAGCGCTCT TCTGAGAGTG CGGAGAAAGC CCTGCAGACG 
GTGCCCAACA TCGAGAAGGA GATTCAGAAT GCGGAAAGCC TGATCAGCCA GGCGGAGGAG 
GCTCTCGATG GAGCCAATAA GAATGCCAAC GAGGCCAAGA AGAATGCCCA GGAGGCGCAG 
CTGAAGTATG CCGAACAGGC TTCCAAGGAT GCTGAGCTGA TCCGCCGCAA AGCCAATGAG 
ACCAAGGTGG CTGCCCGCAA TCTGCGCGAA GAGGCCGACC AACTGAATCA TCGCGTGAAA 
CTCACCGAAA TGGACATCTT CAAGCTGGAG GAGAGCTCGA CCAAGGACGA CAATCTGGTG 
GACGACGCCA AGCGAAAGGT GGGTCAGGCC AAGGCCGATA CCCAGGAGGC CCAGAAGCAG 
ATCGAGAAGG CCAATGCCGA CCTGACGGCC ATCAAGGATG AGCTGGAGAA TCTGAAGGAC 
ATCAACACGG GCGATTTGGA TAGATTGGAG AATCGTTTGG CCACTGTGGA GGGTGAGATC 
AATCGCGTCA ACCTGACGGG ACGCATCGAA AAGTACCGGG AACAGCGCAC CATTCAGAAG 
AATCTGATCG ACAAGTACGA TGCCGAGCTG AGGGAGCTCA AGGATGAAGT CCAGAATATT 
GGACTTATTT CCAAGGCTCT CCCCGATAGC TGCTTCAGTC GCAATCGCCT GGAACCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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LanB2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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