CG5087 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG5087
Entrez Gene ID	39035
Full Name	CG5087 gene product from transcript CG5087-RA
Synonyms	Dmel\CG5087,NM_140022.1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66F1-66F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_140022.2	NP_648279.1	uncharacterized protein, isoform A
NM_001274681.1	NP_001261610.1	uncharacterized protein, isoform B

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	ube3b	NP_001072875.1
Pan troglodytes (chimpanzee)	UBE3B	XP_001137003.2
Danio rerio (zebrafish)	ube3b	NP_001108154.1
	UBE3B	XP_002798818.1
Homo sapiens (human)	UBE3B	NP_569733.2
Canis lupus familiaris (dog)	UBE3B	XP_543437.2
Bos taurus (cattle)	UBE3B	NP_001193161.1
Drosophila melanogaster (fruit fly)	CG5087	NP_001261610.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005897	XP_315926.2
Caenorhabditis elegans (roundworm)	oxi-1	NP_499392.1
Mus musculus (house mouse)	Ube3b	NP_473434.2
Rattus norvegicus (Norway rat)	Ube3b	NP_001137366.1
Gallus gallus (chicken)	LOC776286	XP_003642246.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG5087 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG5087 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17976	NM_140022.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG5087), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa20769	NM_001274681.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG5087), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG5087 ( NM_140022.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648279.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG5087), mRNA.

Price & Availability↑

CloneID	OFa17976
Clone ID Related Accession (Same CDS sequence)	NM_140022.2
Accession Version	NM_140022.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140022.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGCCC AAAACCAAAA GGCCAGCTTC CTGGAGCAGA CAAAGGCGGC GCGGGAGGAG 
CGGGCACTGG AAAAGCGACG CGAACAGGCG GCCATCCTGC TGCAGTCCAC GCTCAAGGGA 
TATGCGGCGC GTAGGAAGTA CCAGAAACGA ATCATACTCG ATTTCGATGC CATGTTCACT 
GAGAATCATG ACGATAAGGA TGCGGAACTG GAACTCGTGG CAAGCAACGT CTATCCGGTG 
CTTCGTCGCT ACCTCACCCA GATCAGGCTG GACAAGAGCG ATGTGCGTGC ACGGGAGCGT 
CTGGAGCGCA TCTGCCGTTA CATCATCAAG GCAATGGAGT CGGAGAACAC CAAGCTATCC 
TATGCCGCCC TCTGTCTTTT TAAGGAACGC AGCCTGCCCT GGATCGCGCA CATCAAGATA 
CTGCTCACGA ACTGCCTGAC ATTGCTGCCG GAGCTGAAGC CGGAAAACCA TGCCGACAGC 
TTGTCCCTTG CCCTATTTCT GCACACACTG GTAGTCTTCA CGGCGCCCAA ATCCTGGGCG 
ATACTCAGGA ATGCCCAGTT TGAGAAACTG CAGCCGGCCA TGCAAAAAAT CTGTTGTAAT 
ATTCAAGGTC ATCTGGTGCA GCACGATTTT TACAAAATCA TGCGGCTCGT TTTGATACGC 
GGCACTGTTC GCGAGGAGTT GTCTGTAAAG CCCGTCACCC TGGTGGCCAT CATTACACTT 
TGCCTTCGTC CCCTGATCGA TGGCAACTTC AGTCGTAATC TACTGGCAAA GTTTCTGTCG 
GAGATTCTGT CTGTGCCCGC TTTAATCTAC CATCTGCAAC AGAGTGTACC TCAGTGCCTG 
GAACAGTTCT CATCGATGGG ACTGCTTAAG AAGGCGCTTA GCATTTCGGG GGACGTGCAG 
TGGTTCGAGG AGTTCGGCAC TAGCATGCCG GGCACCAAGT CCCTGGCTTT TCTGGGAAAC 
ATAGTGAATT TGTTCAATAT CGATGGCCAA GGGGAGTCCA AGGAGTTGGC CTATCCACTA 
CTGACTGAAA CCACCACATC GCTGTTGGAA CTAATACCCA ACACGGTGAC CACTAAGGGA 
GTATTTACTC AGTGGCACGA GCTTTTAGGA TGGCATACTC CGGGTCCGGA GCCTGCTCAA 
AATCAGAATG TGGCGCTGAT TAAGAAGCAG TTCCACATGT TGTGGGATCA TCGCTGCATA 
AAACTGTTGC TCGGAGATCT CTTGAAGCAA ATCAATCTTA ACTACGAGCG GATCGAGTTT 
CAATCTCCCC AACAACCATC TATATCGAAT CTGCTGCGTC GGGCCTTGGA GCGCAGCTCC 
ACGAGAGGTG TAAACTTAAT GGGAGTGGCG AGCAGCAAGC AGTCTAAGCA GCAGTGGCGC 
AAGCTCGACA GTGCGGAGGT GGTGCAGGTG TCACGAATCT GCGGCATGTA TTATGCCGCC 
CTCAACACGC TTTCCCAGAT GAAACTGGAC ATTCTGACTG GCATTTGCTA CAGCGACAAT 
GTGCTATATG ATATTTGGTT GCTAATCACG TCCCTTGGCC CCAATTGTGG CATGAAGGAG 
TACTTGGAGC TGCTGAAGTC CGAAACAAAC TTGCAAAAGC CACAGACCGC CATGCTGATG 
CTCTTTTGCG ACTGCATGAC GCACTACGTA ACGATTCTGG ATGAACACGA AATGTACACG 
GAGCAGAATC CTTTCAAACT CAACGACTAC GTGTTGCTGA CCTACTTCCT TAATAATATT 
CTCTATAAAC TTATTAACGA TAATCTTCTG GCAGGTGCCA AGAACATTGT CCAGCATCCG 
GTTTTCCTCT CGCTGCACAC TCTGATGCTT TGCTTGTACC GCCGCGATTG TCGTCGCCCA 
TTCACGCCAC CCAAACACTG GCTGATACCA GAGGTGAAAC CCTCCACGTT CATCAATGAC 
CTGGAAAAGG CCAAGAGGAA TGCGATGCTG CTGCTCGCCA AGATGCCGCA CATTATACCG 
CACGAGGATC GGGTCAAGTT GTTCCGAAAG TTCGTGCAGA ATGAGAAGGC GGTGATGGGT 
CTGACGGAGA GTGCCTGCGC CTCACCTCGA TCCGCACTAA TTGTCATTCA TCGCGATCGT 
ATTGTGGAGG ATGGCTACCG GCAACTGGCC GCCCAGCCCA CGCAAGCCCT AAAAGGTGTT 
ATCCGCGTGC GTTTTATCAA TCAACAAGGA CTGCACGAGG CAGGAATTGA TCAGGATGGA 
GTGTTCAAGG AATTCCTTGA GGAGACCATC AAAAAGGTGT TCGACCCGTC GCTGAATCTC 
TTTAAAACCA CCTCGGATCA GCGATTATAT CCCTCGCCCA TTTCCTACGT CCAGGACAAT 
CATTTGGAGC TCTTTGAGTT TGTGGGCCGC ATGCTGGGCA AGGCGGTGTA CGAGGGCATC 
GTGGTGGACG TGCCGTTTGC CTCCTTCTTC CTCTCCCAGC TGCTCGGCCA GACGCAACAG 
GCCCTGTACT CCTGCATGGA CGAGCTGCCG TCGCTGGACA ACGAGTTGTA TCGCAGTCTT 
ACCTTCATCA AACACTATAA GCAGGATGTG TCCGATCTGA ACCTTACCTT TTCCGTGGAC 
CAGGATGTAA TGGGCAAAAT TGTCACGCTC GCTTTGCATC CGGGTGGCAA GGCGCGTGTG 
GTTAATGACC ACAACAAGCT GGTCTACATA CACTACATGG CCTTTTTCCA CATGAACACC 
CAGATACGCG AGCAGACGAT AGCTTTCAAT CGTGGCTTCC GCAGCATCGT CAATCCAGAA 
TGGTTGTCTC TTTTCTCGCC TCCCGAATTG CAGCGCCTTA TTTCTGGGGA CACTAGTCCA 
TTGGACTTAA AGGATCTGCA AAAGCATACG CATTACTACG GTGGCTTCCA CGACACTCAT 
CAAGTGGTCT GCTGGCTCTG GGATATCCTG GCCAAGGATT TCACCGAGGA GGAGCGCAAG 
CTGTTCCTAA AGTTTGTCAC CAGCTGCTCA AAGCCGCCGC TTTTGGGTTT CGCCCACCTG 
GAGCCACCAT TCTCGATTCG TTGCGTGGAG GTTAGTGATG ACGAGGATAC AGGCGACACA 
ATCGGAAGTG TGATTCGCGG TTTCTTCGCC ATACGTAAGA AGGATCCACT CAACCGACTG 
CCCACCTCGT CGACCTGCTT CAATCTGCTG AAGCTGCCCA ACTACCAAAA GAAGTCCACG 
CTGCGGGACA AACTTCGCTA TGCCGTAAGC AGCAACACCG GATTCGAGCT GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648279.1
CDS	272..3508
Translation	MFAQNQKASFLEQTKAAREERALEKRREQAAILLQSTLKGYAARRKYQKRIILDFDAMFTENHDDKDAELELVASNVYPVLRRYLTQIRLDKSDVRARERLERICRYIIKAMESENTKLSYAALCLFKERSLPWIAHIKILLTNCLTLLPELKPENHADSLSLALFLHTLVVFTAPKSWAILRNAQFEKLQPAMQKICCNIQGHLVQHDFYKIMRLVLIRGTVREELSVKPVTLVAIITLCLRPLIDGNFSRNLLAKFLSEILSVPALIYHLQQSVPQCLEQFSSMGLLKKALSISGDVQWFEEFGTSMPGTKSLAFLGNIVNLFNIDGQGESKELAYPLLTETTTSLLELIPNTVTTKGVFTQWHELLGWHTPGPEPAQNQNVALIKKQFHMLWDHRCIKLLLGDLLKQINLNYERIEFQSPQQPSISNLLRRALERSSTRGVNLMGVASSKQSKQQWRKLDSAEVVQVSRICGMYYAALNTLSQMKLDILTGICYSDNVLYDIWLLITSLGPNCGMKEYLELLKSETNLQKPQTAMLMLFCDCMTHYVTILDEHEMYTEQNPFKLNDYVLLTYFLNNILYKLINDNLLAGAKNIVQHPVFLSLHTLMLCLYRRDCRRPFTPPKHWLIPEVKPSTFINDLEKAKRNAMLLLAKMPHIIPHEDRVKLFRKFVQNEKAVMGLTESACASPRSALIVIHRDRIVEDGYRQLAAQPTQALKGVIRVRFINQQGLHEAGIDQDGVFKEFLEETIKKVFDPSLNLFKTTSDQRLYPSPISYVQDNHLELFEFVGRMLGKAVYEGIVVDVPFASFFLSQLLGQTQQALYSCMDELPSLDNELYRSLTFIKHYKQDVSDLNLTFSVDQDVMGKIVTLALHPGGKARVVNDHNKLVYIHYMAFFHMNTQIREQTIAFNRGFRSIVNPEWLSLFSPPELQRLISGDTSPLDLKDLQKHTHYYGGFHDTHQVVCWLWDILAKDFTEEERKLFLKFVTSCSKPPLLGFAHLEPPFSIRCVEVSDDEDTGDTIGSVIRGFFAIRKKDPLNRLPTSSTCFNLLKLPNYQKKSTLRDKLRYAVSSNTGFELS

Target ORF information:

RefSeq Version	NM_140022.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG5087), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140022.2

ORF Insert Sequence:

ATGTTTGCCC AAAACCAAAA GGCCAGCTTC CTGGAGCAGA CAAAGGCGGC GCGGGAGGAG 
CGGGCACTGG AAAAGCGACG CGAACAGGCG GCCATCCTGC TGCAGTCCAC GCTCAAGGGA 
TATGCGGCGC GTAGGAAGTA CCAGAAACGA ATCATACTCG ATTTCGATGC CATGTTCACT 
GAGAATCATG ACGATAAGGA TGCGGAACTG GAACTCGTGG CAAGCAACGT CTATCCGGTG 
CTTCGTCGCT ACCTCACCCA GATCAGGCTG GACAAGAGCG ATGTGCGTGC ACGGGAGCGT 
CTGGAGCGCA TCTGCCGTTA CATCATCAAG GCAATGGAGT CGGAGAACAC CAAGCTATCC 
TATGCCGCCC TCTGTCTTTT TAAGGAACGC AGCCTGCCCT GGATCGCGCA CATCAAGATA 
CTGCTCACGA ACTGCCTGAC ATTGCTGCCG GAGCTGAAGC CGGAAAACCA TGCCGACAGC 
TTGTCCCTTG CCCTATTTCT GCACACACTG GTAGTCTTCA CGGCGCCCAA ATCCTGGGCG 
ATACTCAGGA ATGCCCAGTT TGAGAAACTG CAGCCGGCCA TGCAAAAAAT CTGTTGTAAT 
ATTCAAGGTC ATCTGGTGCA GCACGATTTT TACAAAATCA TGCGGCTCGT TTTGATACGC 
GGCACTGTTC GCGAGGAGTT GTCTGTAAAG CCCGTCACCC TGGTGGCCAT CATTACACTT 
TGCCTTCGTC CCCTGATCGA TGGCAACTTC AGTCGTAATC TACTGGCAAA GTTTCTGTCG 
GAGATTCTGT CTGTGCCCGC TTTAATCTAC CATCTGCAAC AGAGTGTACC TCAGTGCCTG 
GAACAGTTCT CATCGATGGG ACTGCTTAAG AAGGCGCTTA GCATTTCGGG GGACGTGCAG 
TGGTTCGAGG AGTTCGGCAC TAGCATGCCG GGCACCAAGT CCCTGGCTTT TCTGGGAAAC 
ATAGTGAATT TGTTCAATAT CGATGGCCAA GGGGAGTCCA AGGAGTTGGC CTATCCACTA 
CTGACTGAAA CCACCACATC GCTGTTGGAA CTAATACCCA ACACGGTGAC CACTAAGGGA 
GTATTTACTC AGTGGCACGA GCTTTTAGGA TGGCATACTC CGGGTCCGGA GCCTGCTCAA 
AATCAGAATG TGGCGCTGAT TAAGAAGCAG TTCCACATGT TGTGGGATCA TCGCTGCATA 
AAACTGTTGC TCGGAGATCT CTTGAAGCAA ATCAATCTTA ACTACGAGCG GATCGAGTTT 
CAATCTCCCC AACAACCATC TATATCGAAT CTGCTGCGTC GGGCCTTGGA GCGCAGCTCC 
ACGAGAGGTG TAAACTTAAT GGGAGTGGCG AGCAGCAAGC AGTCTAAGCA GCAGTGGCGC 
AAGCTCGACA GTGCGGAGGT GGTGCAGGTG TCACGAATCT GCGGCATGTA TTATGCCGCC 
CTCAACACGC TTTCCCAGAT GAAACTGGAC ATTCTGACTG GCATTTGCTA CAGCGACAAT 
GTGCTATATG ATATTTGGTT GCTAATCACG TCCCTTGGCC CCAATTGTGG CATGAAGGAG 
TACTTGGAGC TGCTGAAGTC CGAAACAAAC TTGCAAAAGC CACAGACCGC CATGCTGATG 
CTCTTTTGCG ACTGCATGAC GCACTACGTA ACGATTCTGG ATGAACACGA AATGTACACG 
GAGCAGAATC CTTTCAAACT CAACGACTAC GTGTTGCTGA CCTACTTCCT TAATAATATT 
CTCTATAAAC TTATTAACGA TAATCTTCTG GCAGGTGCCA AGAACATTGT CCAGCATCCG 
GTTTTCCTCT CGCTGCACAC TCTGATGCTT TGCTTGTACC GCCGCGATTG TCGTCGCCCA 
TTCACGCCAC CCAAACACTG GCTGATACCA GAGGTGAAAC CCTCCACGTT CATCAATGAC 
CTGGAAAAGG CCAAGAGGAA TGCGATGCTG CTGCTCGCCA AGATGCCGCA CATTATACCG 
CACGAGGATC GGGTCAAGTT GTTCCGAAAG TTCGTGCAGA ATGAGAAGGC GGTGATGGGT 
CTGACGGAGA GTGCCTGCGC CTCACCTCGA TCCGCACTAA TTGTCATTCA TCGCGATCGT 
ATTGTGGAGG ATGGCTACCG GCAACTGGCC GCCCAGCCCA CGCAAGCCCT AAAAGGTGTT 
ATCCGCGTGC GTTTTATCAA TCAACAAGGA CTGCACGAGG CAGGAATTGA TCAGGATGGA 
GTGTTCAAGG AATTCCTTGA GGAGACCATC AAAAAGGTGT TCGACCCGTC GCTGAATCTC 
TTTAAAACCA CCTCGGATCA GCGATTATAT CCCTCGCCCA TTTCCTACGT CCAGGACAAT 
CATTTGGAGC TCTTTGAGTT TGTGGGCCGC ATGCTGGGCA AGGCGGTGTA CGAGGGCATC 
GTGGTGGACG TGCCGTTTGC CTCCTTCTTC CTCTCCCAGC TGCTCGGCCA GACGCAACAG 
GCCCTGTACT CCTGCATGGA CGAGCTGCCG TCGCTGGACA ACGAGTTGTA TCGCAGTCTT 
ACCTTCATCA AACACTATAA GCAGGATGTG TCCGATCTGA ACCTTACCTT TTCCGTGGAC 
CAGGATGTAA TGGGCAAAAT TGTCACGCTC GCTTTGCATC CGGGTGGCAA GGCGCGTGTG 
GTTAATGACC ACAACAAGCT GGTCTACATA CACTACATGG CCTTTTTCCA CATGAACACC 
CAGATACGCG AGCAGACGAT AGCTTTCAAT CGTGGCTTCC GCAGCATCGT CAATCCAGAA 
TGGTTGTCTC TTTTCTCGCC TCCCGAATTG CAGCGCCTTA TTTCTGGGGA CACTAGTCCA 
TTGGACTTAA AGGATCTGCA AAAGCATACG CATTACTACG GTGGCTTCCA CGACACTCAT 
CAAGTGGTCT GCTGGCTCTG GGATATCCTG GCCAAGGATT TCACCGAGGA GGAGCGCAAG 
CTGTTCCTAA AGTTTGTCAC CAGCTGCTCA AAGCCGCCGC TTTTGGGTTT CGCCCACCTG 
GAGCCACCAT TCTCGATTCG TTGCGTGGAG GTTAGTGATG ACGAGGATAC AGGCGACACA 
ATCGGAAGTG TGATTCGCGG TTTCTTCGCC ATACGTAAGA AGGATCCACT CAACCGACTG 
CCCACCTCGT CGACCTGCTT CAATCTGCTG AAGCTGCCCA ACTACCAAAA GAAGTCCACG 
CTGCGGGACA AACTTCGCTA TGCCGTAAGC AGCAACACCG GATTCGAGCT GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG5087 ( NM_001274681.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261610.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG5087), mRNA.

Price & Availability↑

CloneID	OFa20769
Clone ID Related Accession (Same CDS sequence)	NM_001274681.1
Accession Version	NM_001274681.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGCCC AAAACCAAAA GGCCAGCTTC CTGGAGCAGA CAAAGGCGGC GCGGGAGGAG 
CGGGCACTGG AAAAGCGACG CGAACAGGCG GCCATCCTGC TGCAGTCCAC GCTCAAGGGA 
TATGCGGCGC GTAGGAAGTA CCAGAAACGA ATCATACTCG ATTTCGATGC CATGTTCACT 
GAGAATCATG ACGATAAGGA TGCGGAACTG GAACTCGTGG CAAGCAACGT CTATCCGGTG 
CTTCGTCGCT ACCTCACCCA GATCAGGCTG GACAAGAGCG ATGTGCGTGC ACGGGAGCGT 
CTGGAGCGCA TCTGCCGTTA CATCATCAAG GCAATGGAGT CGGAGAACAC CAAGCTATCC 
TATGCCGCCC TCTGTCTTTT TAAGGAACGC AGCCTGCCCT GGATCGCGCA CATCAAGATA 
CTGCTCACGA ACTGCCTGAC ATTGCTGCCG GAGCTGAAGC CGGAAAACCA TGCCGACAGC 
TTGTCCCTTG CCCTATTTCT GCACACACTG GTAGTCTTCA CGGCGCCCAA ATCCTGGGCG 
ATACTCAGGA ATGCCCAGTT TGAGAAACTG CAGCCGGCCA TGCAAAAAAT CTGTTGTAAT 
ATTCAAGGTC ATCTGGTGCA GCACGATTTT TACAAAATCA TGCGGCTCGT TTTGATACGC 
GGCACTGTTC GCGAGGAGTT GTCTGTAAAG CCCGTCACCC TGGTGGCCAT CATTACACTT 
TGCCTTCGTC CCCTGATCGA TGGCAACTTC AGTCGTAATC TACTGGCAAA GTTTCTGTCG 
GAGATTCTGT CTGTGCCCGC TTTAATCTAC CATCTGCAAC AGAGTGTACC TCAGTGCCTG 
GAACAGTTCT CATCGATGGG ACTGCTTAAG AAGGCGCTTA GCATTTCGGG GGACGTGCAG 
TGGTTCGAGG AGTTCGGCAC TAGCATGCCG GGCACCAAGT CCCTGGCTTT TCTGGGAAAC 
ATAGTGAATT TGTTCAATAT CGATGGCCAA GGGGAGTCCA AGGAGTTGGC CTATCCACTA 
CTGACTGAAA CCACCACATC GCTGTTGGAA CTAATACCCA ACACGGTGAC CACTAAGGGA 
GTATTTACTC AGTGGCACGA GCTTTTAGGA TGGCATACTC CGGGTCCGGA GCCTGCTCAA 
AATCAGAATG TGGCGCTGAT TAAGAAGCAG TTCCACATGT TGTGGGATCA TCGCTGCATA 
AAACTGTTGC TCGGAGATCT CTTGAAGCAA ATCAATCTTA ACTACGAGCG GATCGAGTTT 
CAATCTCCCC AACAACCATC TATATCGAAT CTGCTGCGTC GGGCCTTGGA GCGCAGCTCC 
ACGAGAGGTG TAAACTTAAT GGGAGTGGCG AGCAGCAAGC AGTCTAAGCA GCAGTGGCGC 
AAGCTCGACA GTGCGGAGGT GGTGCAGGTG TCACGAATCT GCGGCATGTA TTATGCCGCC 
CTCAACACGC TTTCCCAGAT GAAACTGGAC ATTCTGACTG GCATTTGCTA CAGCGACAAT 
GTGCTATATG ATATTTGGTT GCTAATCACG TCCCTTGGCC CCAATTGTGG CATGAAGGAG 
TACTTGGAGC TGCTGAAGTC CGAAACAAAC TTGCAAAAGC CACAGACCGC CATGCTGATG 
CTCTTTTGCG ACTGCATGAC GCACTACGTA ACGATTCTGG ATGAACACGA AATGTACACG 
GAGCAGAATC CTTTCAAACT CAACGACTAC GTGTTGCTGA CCTACTTCCT TAATAATATT 
CTCTATAAAC TTATTAACGA TAATCTTCTG GGTGCCAAGA ACATTGTCCA GCATCCGGTT 
TTCCTCTCGC TGCACACTCT GATGCTTTGC TTGTACCGCC GCGATTGTCG TCGCCCATTC 
ACGCCACCCA AACACTGGCT GATACCAGAG GTGAAACCCT CCACGTTCAT CAATGACCTG 
GAAAAGGCCA AGAGGAATGC GATGCTGCTG CTCGCCAAGA TGCCGCACAT TATACCGCAC 
GAGGATCGGG TCAAGTTGTT CCGAAAGTTC GTGCAGAATG AGAAGGCGGT GATGGGTCTG 
ACGGAGAGTG CCTGCGCCTC ACCTCGATCC GCACTAATTG TCATTCATCG CGATCGTATT 
GTGGAGGATG GCTACCGGCA ACTGGCCGCC CAGCCCACGC AAGCCCTAAA AGGTGTTATC 
CGCGTGCGTT TTATCAATCA ACAAGGACTG CACGAGGCAG GAATTGATCA GGATGGAGTG 
TTCAAGGAAT TCCTTGAGGA GACCATCAAA AAGGTGTTCG ACCCGTCGCT GAATCTCTTT 
AAAACCACCT CGGATCAGCG ATTATATCCC TCGCCCATTT CCTACGTCCA GGACAATCAT 
TTGGAGCTCT TTGAGTTTGT GGGCCGCATG CTGGGCAAGG CGGTGTACGA GGGCATCGTG 
GTGGACGTGC CGTTTGCCTC CTTCTTCCTC TCCCAGCTGC TCGGCCAGAC GCAACAGGCC 
CTGTACTCCT GCATGGACGA GCTGCCGTCG CTGGACAACG AGTTGTATCG CAGTCTTACC 
TTCATCAAAC ACTATAAGCA GGATGTGTCC GATCTGAACC TTACCTTTTC CGTGGACCAG 
GATGTAATGG GCAAAATTGT CACGCTCGCT TTGCATCCGG GTGGCAAGGC GCGTGTGGTT 
AATGACCACA ACAAGCTGGT CTACATACAC TACATGGCCT TTTTCCACAT GAACACCCAG 
ATACGCGAGC AGACGATAGC TTTCAATCGT GGCTTCCGCA GCATCGTCAA TCCAGAATGG 
TTGTCTCTTT TCTCGCCTCC CGAATTGCAG CGCCTTATTT CTGGGGACAC TAGTCCATTG 
GACTTAAAGG ATCTGCAAAA GCATACGCAT TACTACGGTG GCTTCCACGA CACTCATCAA 
GTGGTCTGCT GGCTCTGGGA TATCCTGGCC AAGGATTTCA CCGAGGAGGA GCGCAAGCTG 
TTCCTAAAGT TTGTCACCAG CTGCTCAAAG CCGCCGCTTT TGGGTTTCGC CCACCTGGAG 
CCACCATTCT CGATTCGTTG CGTGGAGGTT AGTGATGACG AGGATACAGG CGACACAATC 
GGAAGTGTGA TTCGCGGTTT CTTCGCCATA CGTAAGAAGG ATCCACTCAA CCGACTGCCC 
ACCTCGTCGA CCTGCTTCAA TCTGCTGAAG CTGCCCAACT ACCAAAAGAA GTCCACGCTG 
CGGGACAAAC TTCGCTATGC CGTAAGCAGC AACACCGGAT TCGAGCTGTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261610.1
CDS	272..3505
Translation	MFAQNQKASFLEQTKAAREERALEKRREQAAILLQSTLKGYAARRKYQKRIILDFDAMFTENHDDKDAELELVASNVYPVLRRYLTQIRLDKSDVRARERLERICRYIIKAMESENTKLSYAALCLFKERSLPWIAHIKILLTNCLTLLPELKPENHADSLSLALFLHTLVVFTAPKSWAILRNAQFEKLQPAMQKICCNIQGHLVQHDFYKIMRLVLIRGTVREELSVKPVTLVAIITLCLRPLIDGNFSRNLLAKFLSEILSVPALIYHLQQSVPQCLEQFSSMGLLKKALSISGDVQWFEEFGTSMPGTKSLAFLGNIVNLFNIDGQGESKELAYPLLTETTTSLLELIPNTVTTKGVFTQWHELLGWHTPGPEPAQNQNVALIKKQFHMLWDHRCIKLLLGDLLKQINLNYERIEFQSPQQPSISNLLRRALERSSTRGVNLMGVASSKQSKQQWRKLDSAEVVQVSRICGMYYAALNTLSQMKLDILTGICYSDNVLYDIWLLITSLGPNCGMKEYLELLKSETNLQKPQTAMLMLFCDCMTHYVTILDEHEMYTEQNPFKLNDYVLLTYFLNNILYKLINDNLLGAKNIVQHPVFLSLHTLMLCLYRRDCRRPFTPPKHWLIPEVKPSTFINDLEKAKRNAMLLLAKMPHIIPHEDRVKLFRKFVQNEKAVMGLTESACASPRSALIVIHRDRIVEDGYRQLAAQPTQALKGVIRVRFINQQGLHEAGIDQDGVFKEFLEETIKKVFDPSLNLFKTTSDQRLYPSPISYVQDNHLELFEFVGRMLGKAVYEGIVVDVPFASFFLSQLLGQTQQALYSCMDELPSLDNELYRSLTFIKHYKQDVSDLNLTFSVDQDVMGKIVTLALHPGGKARVVNDHNKLVYIHYMAFFHMNTQIREQTIAFNRGFRSIVNPEWLSLFSPPELQRLISGDTSPLDLKDLQKHTHYYGGFHDTHQVVCWLWDILAKDFTEEERKLFLKFVTSCSKPPLLGFAHLEPPFSIRCVEVSDDEDTGDTIGSVIRGFFAIRKKDPLNRLPTSSTCFNLLKLPNYQKKSTLRDKLRYAVSSNTGFELS

Target ORF information:

RefSeq Version	NM_001274681.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG5087), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274681.1

ORF Insert Sequence:

ATGTTTGCCC AAAACCAAAA GGCCAGCTTC CTGGAGCAGA CAAAGGCGGC GCGGGAGGAG 
CGGGCACTGG AAAAGCGACG CGAACAGGCG GCCATCCTGC TGCAGTCCAC GCTCAAGGGA 
TATGCGGCGC GTAGGAAGTA CCAGAAACGA ATCATACTCG ATTTCGATGC CATGTTCACT 
GAGAATCATG ACGATAAGGA TGCGGAACTG GAACTCGTGG CAAGCAACGT CTATCCGGTG 
CTTCGTCGCT ACCTCACCCA GATCAGGCTG GACAAGAGCG ATGTGCGTGC ACGGGAGCGT 
CTGGAGCGCA TCTGCCGTTA CATCATCAAG GCAATGGAGT CGGAGAACAC CAAGCTATCC 
TATGCCGCCC TCTGTCTTTT TAAGGAACGC AGCCTGCCCT GGATCGCGCA CATCAAGATA 
CTGCTCACGA ACTGCCTGAC ATTGCTGCCG GAGCTGAAGC CGGAAAACCA TGCCGACAGC 
TTGTCCCTTG CCCTATTTCT GCACACACTG GTAGTCTTCA CGGCGCCCAA ATCCTGGGCG 
ATACTCAGGA ATGCCCAGTT TGAGAAACTG CAGCCGGCCA TGCAAAAAAT CTGTTGTAAT 
ATTCAAGGTC ATCTGGTGCA GCACGATTTT TACAAAATCA TGCGGCTCGT TTTGATACGC 
GGCACTGTTC GCGAGGAGTT GTCTGTAAAG CCCGTCACCC TGGTGGCCAT CATTACACTT 
TGCCTTCGTC CCCTGATCGA TGGCAACTTC AGTCGTAATC TACTGGCAAA GTTTCTGTCG 
GAGATTCTGT CTGTGCCCGC TTTAATCTAC CATCTGCAAC AGAGTGTACC TCAGTGCCTG 
GAACAGTTCT CATCGATGGG ACTGCTTAAG AAGGCGCTTA GCATTTCGGG GGACGTGCAG 
TGGTTCGAGG AGTTCGGCAC TAGCATGCCG GGCACCAAGT CCCTGGCTTT TCTGGGAAAC 
ATAGTGAATT TGTTCAATAT CGATGGCCAA GGGGAGTCCA AGGAGTTGGC CTATCCACTA 
CTGACTGAAA CCACCACATC GCTGTTGGAA CTAATACCCA ACACGGTGAC CACTAAGGGA 
GTATTTACTC AGTGGCACGA GCTTTTAGGA TGGCATACTC CGGGTCCGGA GCCTGCTCAA 
AATCAGAATG TGGCGCTGAT TAAGAAGCAG TTCCACATGT TGTGGGATCA TCGCTGCATA 
AAACTGTTGC TCGGAGATCT CTTGAAGCAA ATCAATCTTA ACTACGAGCG GATCGAGTTT 
CAATCTCCCC AACAACCATC TATATCGAAT CTGCTGCGTC GGGCCTTGGA GCGCAGCTCC 
ACGAGAGGTG TAAACTTAAT GGGAGTGGCG AGCAGCAAGC AGTCTAAGCA GCAGTGGCGC 
AAGCTCGACA GTGCGGAGGT GGTGCAGGTG TCACGAATCT GCGGCATGTA TTATGCCGCC 
CTCAACACGC TTTCCCAGAT GAAACTGGAC ATTCTGACTG GCATTTGCTA CAGCGACAAT 
GTGCTATATG ATATTTGGTT GCTAATCACG TCCCTTGGCC CCAATTGTGG CATGAAGGAG 
TACTTGGAGC TGCTGAAGTC CGAAACAAAC TTGCAAAAGC CACAGACCGC CATGCTGATG 
CTCTTTTGCG ACTGCATGAC GCACTACGTA ACGATTCTGG ATGAACACGA AATGTACACG 
GAGCAGAATC CTTTCAAACT CAACGACTAC GTGTTGCTGA CCTACTTCCT TAATAATATT 
CTCTATAAAC TTATTAACGA TAATCTTCTG GGTGCCAAGA ACATTGTCCA GCATCCGGTT 
TTCCTCTCGC TGCACACTCT GATGCTTTGC TTGTACCGCC GCGATTGTCG TCGCCCATTC 
ACGCCACCCA AACACTGGCT GATACCAGAG GTGAAACCCT CCACGTTCAT CAATGACCTG 
GAAAAGGCCA AGAGGAATGC GATGCTGCTG CTCGCCAAGA TGCCGCACAT TATACCGCAC 
GAGGATCGGG TCAAGTTGTT CCGAAAGTTC GTGCAGAATG AGAAGGCGGT GATGGGTCTG 
ACGGAGAGTG CCTGCGCCTC ACCTCGATCC GCACTAATTG TCATTCATCG CGATCGTATT 
GTGGAGGATG GCTACCGGCA ACTGGCCGCC CAGCCCACGC AAGCCCTAAA AGGTGTTATC 
CGCGTGCGTT TTATCAATCA ACAAGGACTG CACGAGGCAG GAATTGATCA GGATGGAGTG 
TTCAAGGAAT TCCTTGAGGA GACCATCAAA AAGGTGTTCG ACCCGTCGCT GAATCTCTTT 
AAAACCACCT CGGATCAGCG ATTATATCCC TCGCCCATTT CCTACGTCCA GGACAATCAT 
TTGGAGCTCT TTGAGTTTGT GGGCCGCATG CTGGGCAAGG CGGTGTACGA GGGCATCGTG 
GTGGACGTGC CGTTTGCCTC CTTCTTCCTC TCCCAGCTGC TCGGCCAGAC GCAACAGGCC 
CTGTACTCCT GCATGGACGA GCTGCCGTCG CTGGACAACG AGTTGTATCG CAGTCTTACC 
TTCATCAAAC ACTATAAGCA GGATGTGTCC GATCTGAACC TTACCTTTTC CGTGGACCAG 
GATGTAATGG GCAAAATTGT CACGCTCGCT TTGCATCCGG GTGGCAAGGC GCGTGTGGTT 
AATGACCACA ACAAGCTGGT CTACATACAC TACATGGCCT TTTTCCACAT GAACACCCAG 
ATACGCGAGC AGACGATAGC TTTCAATCGT GGCTTCCGCA GCATCGTCAA TCCAGAATGG 
TTGTCTCTTT TCTCGCCTCC CGAATTGCAG CGCCTTATTT CTGGGGACAC TAGTCCATTG 
GACTTAAAGG ATCTGCAAAA GCATACGCAT TACTACGGTG GCTTCCACGA CACTCATCAA 
GTGGTCTGCT GGCTCTGGGA TATCCTGGCC AAGGATTTCA CCGAGGAGGA GCGCAAGCTG 
TTCCTAAAGT TTGTCACCAG CTGCTCAAAG CCGCCGCTTT TGGGTTTCGC CCACCTGGAG 
CCACCATTCT CGATTCGTTG CGTGGAGGTT AGTGATGACG AGGATACAGG CGACACAATC 
GGAAGTGTGA TTCGCGGTTT CTTCGCCATA CGTAAGAAGG ATCCACTCAA CCGACTGCCC 
ACCTCGTCGA CCTGCTTCAA TCTGCTGAAG CTGCCCAACT ACCAAAAGAA GTCCACGCTG 
CGGGACAAAC TTCGCTATGC CGTAAGCAGC AACACCGGAT TCGAGCTGTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG5087 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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