pix cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pix
Entrez Gene ID	39027
Full Name	pixie
Synonyms	ABCE1,BEST:GH18088,CG5651,Dmel\CG5651,E3-c
General protein information	Preferred Names pixie Names CG5651 gene product from transcript CG5651-RA CG5651-PA CG5651-PB pix-PA pix-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66E6-66E6

mRNA and Protein(s)

mRNA	Protein	Name
NM_140015.3	NP_648272.1	pixie, isoform A
NM_168306.2	NP_729435.1	pixie, isoform B

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AGR125W	NP_986791.1
Drosophila melanogaster (fruit fly)	pix	NP_729435.1
Magnaporthe oryzae (rice blast fungus)	MGG_11382	XP_003713614.1
	ABCE1	XP_001095781.1
Mus musculus (house mouse)	Abce1	NP_056566.2
Gallus gallus (chicken)	ABCE1	NP_001006440.1
Kluyveromyces lactis	KLLA0C17556g	XP_452984.1
Schizosaccharomyces pombe (fission yeast)	rli1	NP_596732.1
Arabidopsis thaliana (thale cress)	RLI2	NP_193656.2
Canis lupus familiaris (dog)	ABCE1	XP_867212.2
Rattus norvegicus (Norway rat)	Abce1	NP_001101916.1
Danio rerio (zebrafish)	abce1	NP_998216.2
Caenorhabditis elegans (roundworm)	abce-1	NP_499717.1
Oryza sativa (rice)	Os11g0546000	NP_001068062.1
Xenopus tropicalis (tropical clawed frog)	abce1	NP_001005628.1
Homo sapiens (human)	ABCE1	NP_001035809.1
Pan troglodytes (chimpanzee)	ABCE1	NP_001233538.1
Bos taurus (cattle)	ABCE1	NP_001077154.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002182	XP_308004.3
Saccharomyces cerevisiae (baker's yeast)	RLI1	NP_010376.3
Neurospora crassa	NCU03061	XP_963869.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that Nrx-1 plays critical roles in regulating synaptic terminal clustering and release of synaptic vesicles of the neuromuscular junction; Nrx-1 controls terminal clustering and release of synaptic vesicles by stimulating presynaptic actin cytoskeleton assembly; Nrx-1 functions via Scribble and Pixie to activate Rac1. (Nrx-1 = neurexin-1; Rac1 = GTP-binding protein Rac1)
Title: The neuronal protein Neurexin directly interacts with the Scribble-Pix complex to stimulate F-actin assembly for synaptic vesicle clustering.

Data indicate a functional role of ABCE1 (CG5651; pixie) protein in nonstop mRNA decay (NSD).
Title: A functional involvement of ABCE1, eukaryotic ribosome recycling factor, in nonstop mRNA decay in Drosophila melanogaster cells.

The role of Pixie is not merely to allow growth, as might be expected for a translation regulator. Instead, Pixie also behaves as a target of putative constraining signals that slow disc growth during late larval life.
Title: Growth and cell survival are unevenly impaired in pixie mutant wing discs.

The following pix gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pix cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17967	NM_168306.2 Latest version!	Drosophila melanogaster pixie (pix), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa17967	NM_140015.3 Latest version!	Drosophila melanogaster pixie (pix), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pix ( NM_140015.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648272.1 Drosophila melanogaster pixie (pix), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17967
Clone ID Related Accession (Same CDS sequence)	NM_140015.3 , NM_168306.2
Accession Version	NM_140015.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pixie, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140015.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCGCA GAAAGGAGAA CGAGGAGGTG GACAAGCAGA CGCGTATTGC CATCGTCAGC 
GATGACAAGT GCAAGCCCAA ACGGTGTCGG CAGGAGTGCA AGAAGACCTG CCCAGTGGTG 
CGCATGGGCA AGCTCTGCAT CGAGGTGACG CCCACGTCCA AGATAGCCTC GTTGTCCGAG 
GAGCTGTGCA TCGGTTGCGG TATTTGCGTC AAGAAATGCC CCTTCGAGGC CATCACAATC 
ATCAACTTGC CCAGCAATCT GGAGAAGCAC ACAACGCATC GATACAGCAA GAACTCCTTC 
AAGCTGCATC GTCTGCCCAT TCCGCGTCCC GGTGAAGTGC TCGGTCTTGT GGGACAGAAC 
GGTATTGGCA AGTCGACCGC TTTGAAGATC CTCGCGGGGA AACAAAAGCC GAACCTGGGG 
AAGTACGCCA ATCCGCCCGA CTGGACGGAG ATCCTTAGCT ACTTCCGCGG CTCCGAGCTG 
CAGAACTACT TCACCAAGAT CTTGGAGGAC AATCTGAAGG CGCTGGTCAA GCCGCAGTAT 
GTGGATCAGA TCCCCAAGGC GGTGCGTGGC GCTGTGGGCG ATCTTCTGGA TAAGAAGGAC 
GAGAGGGAGC TGCAGACTAA GATTTGTGAA ATGCTGGATC TATCGCACAT CCGTGATCGT 
GAGATCGCTC AGCTTTCTGG CGGAGAACTG CAACGCTTTG CCATCGCCAT GGTATGCATA 
CAGAATGCAG ACATCTTCAT GTTCGACGAG CCGTCCTCGT ATTTGGATGT TAAGCAGCGT 
CTGAACGCTG CCTTGACCAT TCGATCACTC CTGCATCCCA CCAAGTTTAT TATTGTGGTG 
GAGCACGATT TGTCTGTGCT GGATTACTTG TCTGACTTTA TATGCTGCCT TTACGGAGTT 
CCCGGCTGCT ACGGTGTGGT TACTATGCCT TTCTCTGTGC GCGAAGGCAT CAACATTTTC 
CTCGATGGCT TTGTGCCCAC CGAAAACATG CGATTCCGCA CTGAGTCACT TACTTTCAAA 
GTATCTGAGT CCGCTACGGA GGAGGAGATC AAGCGCATGA ACCACTACGT ATATCCCGCC 
ATGGTCAAGA CCCTGGGCAA GTTCGAGTTG ACGGTGGAAA AGGGTCACTT CAGCGACTCC 
GAGATCCTGG TGCTCCTGGG CGAGAACGGT ACAGGAAAGA CGACGTTCAT TCGCATGCTG 
GCCGGTAACC TCCAACCCGA TGGCGAGGTG GAACTGCCTA TGCTGAATAT TTCCTACAAG 
CCCCAGAAGA TTTCCCCGAA ATTCCAAAAT CATGTTCGCC ATTTATTGCA CGATAAGATT 
CGTGACGCCT ACGTGCATCC TCAGTTTATT GCCGATGTTA TGAAACCCAT GAAAATTGAG 
GAAATCATGG ACCAGGAGGT GCAGAACCTT TCCGGTGGTG AGTTGCAGCG AGTCGCCTTG 
GTCCTGTGTC TTGGCAAGCC AGCGGATGTC TACCTCATCG ACGAACCCTC TGCCTACTTG 
GATTCAGAGC AGCGTCTGGT GGCCGCCAAG GTTATCAAAC GTTACATTCT GCACGCCAAG 
AAAACTGGAT TTGTGGTGGA GCACGATTTC ATCATGGCCA CCTACCTCGC CGACCGTGTC 
ATCGTGATCG AGGGTCAGCC TTCAGTGAAG ACGACAGCCT TTTCGCCGCA GTCGCTGCTA 
AATGGCATGA ACCGCTTCCT CGAACTGCTT GGTATTACGT TCCGTCGCGA TCCCAACAAC 
TTCAGACCCC GCATTAACAA AAACAATTCG GTCAAGGACA CAGAACAGAA ACGCTCCGGC 
CAGTTCTTCT TCTTGGAGGA CGAAGCCTGC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648272.1
CDS	111..1946
Translation	MSRRKENEEVDKQTRIAIVSDDKCKPKRCRQECKKTCPVVRMGKLCIEVTPTSKIASLSEELCIGCGICVKKCPFEAITIINLPSNLEKHTTHRYSKNSFKLHRLPIPRPGEVLGLVGQNGIGKSTALKILAGKQKPNLGKYANPPDWTEILSYFRGSELQNYFTKILEDNLKALVKPQYVDQIPKAVRGAVGDLLDKKDERELQTKICEMLDLSHIRDREIAQLSGGELQRFAIAMVCIQNADIFMFDEPSSYLDVKQRLNAALTIRSLLHPTKFIIVVEHDLSVLDYLSDFICCLYGVPGCYGVVTMPFSVREGINIFLDGFVPTENMRFRTESLTFKVSESATEEEIKRMNHYVYPAMVKTLGKFELTVEKGHFSDSEILVLLGENGTGKTTFIRMLAGNLQPDGEVELPMLNISYKPQKISPKFQNHVRHLLHDKIRDAYVHPQFIADVMKPMKIEEIMDQEVQNLSGGELQRVALVLCLGKPADVYLIDEPSAYLDSEQRLVAAKVIKRYILHAKKTGFVVEHDFIMATYLADRVIVIEGQPSVKTTAFSPQSLLNGMNRFLELLGITFRRDPNNFRPRINKNNSVKDTEQKRSGQFFFLEDEACN

Target ORF information:

RefSeq Version	NM_140015.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pixie (pix), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140015.3

ORF Insert Sequence:

ATGTCGCGCA GAAAGGAGAA CGAGGAGGTG GACAAGCAGA CGCGTATTGC CATCGTCAGC 
GATGACAAGT GCAAGCCCAA ACGGTGTCGG CAGGAGTGCA AGAAGACCTG CCCAGTGGTG 
CGCATGGGCA AGCTCTGCAT CGAGGTGACG CCCACGTCCA AGATAGCCTC GTTGTCCGAG 
GAGCTGTGCA TCGGTTGCGG TATTTGCGTC AAGAAATGCC CCTTCGAGGC CATCACAATC 
ATCAACTTGC CCAGCAATCT GGAGAAGCAC ACAACGCATC GATACAGCAA GAACTCCTTC 
AAGCTGCATC GTCTGCCCAT TCCGCGTCCC GGTGAAGTGC TCGGTCTTGT GGGACAGAAC 
GGTATTGGCA AGTCGACCGC TTTGAAGATC CTCGCGGGGA AACAAAAGCC GAACCTGGGG 
AAGTACGCCA ATCCGCCCGA CTGGACGGAG ATCCTTAGCT ACTTCCGCGG CTCCGAGCTG 
CAGAACTACT TCACCAAGAT CTTGGAGGAC AATCTGAAGG CGCTGGTCAA GCCGCAGTAT 
GTGGATCAGA TCCCCAAGGC GGTGCGTGGC GCTGTGGGCG ATCTTCTGGA TAAGAAGGAC 
GAGAGGGAGC TGCAGACTAA GATTTGTGAA ATGCTGGATC TATCGCACAT CCGTGATCGT 
GAGATCGCTC AGCTTTCTGG CGGAGAACTG CAACGCTTTG CCATCGCCAT GGTATGCATA 
CAGAATGCAG ACATCTTCAT GTTCGACGAG CCGTCCTCGT ATTTGGATGT TAAGCAGCGT 
CTGAACGCTG CCTTGACCAT TCGATCACTC CTGCATCCCA CCAAGTTTAT TATTGTGGTG 
GAGCACGATT TGTCTGTGCT GGATTACTTG TCTGACTTTA TATGCTGCCT TTACGGAGTT 
CCCGGCTGCT ACGGTGTGGT TACTATGCCT TTCTCTGTGC GCGAAGGCAT CAACATTTTC 
CTCGATGGCT TTGTGCCCAC CGAAAACATG CGATTCCGCA CTGAGTCACT TACTTTCAAA 
GTATCTGAGT CCGCTACGGA GGAGGAGATC AAGCGCATGA ACCACTACGT ATATCCCGCC 
ATGGTCAAGA CCCTGGGCAA GTTCGAGTTG ACGGTGGAAA AGGGTCACTT CAGCGACTCC 
GAGATCCTGG TGCTCCTGGG CGAGAACGGT ACAGGAAAGA CGACGTTCAT TCGCATGCTG 
GCCGGTAACC TCCAACCCGA TGGCGAGGTG GAACTGCCTA TGCTGAATAT TTCCTACAAG 
CCCCAGAAGA TTTCCCCGAA ATTCCAAAAT CATGTTCGCC ATTTATTGCA CGATAAGATT 
CGTGACGCCT ACGTGCATCC TCAGTTTATT GCCGATGTTA TGAAACCCAT GAAAATTGAG 
GAAATCATGG ACCAGGAGGT GCAGAACCTT TCCGGTGGTG AGTTGCAGCG AGTCGCCTTG 
GTCCTGTGTC TTGGCAAGCC AGCGGATGTC TACCTCATCG ACGAACCCTC TGCCTACTTG 
GATTCAGAGC AGCGTCTGGT GGCCGCCAAG GTTATCAAAC GTTACATTCT GCACGCCAAG 
AAAACTGGAT TTGTGGTGGA GCACGATTTC ATCATGGCCA CCTACCTCGC CGACCGTGTC 
ATCGTGATCG AGGGTCAGCC TTCAGTGAAG ACGACAGCCT TTTCGCCGCA GTCGCTGCTA 
AATGGCATGA ACCGCTTCCT CGAACTGCTT GGTATTACGT TCCGTCGCGA TCCCAACAAC 
TTCAGACCCC GCATTAACAA AAACAATTCG GTCAAGGACA CAGAACAGAA ACGCTCCGGC 
CAGTTCTTCT TCTTGGAGGA CGAAGCCTGC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pix ( NM_168306.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729435.1 Drosophila melanogaster pixie (pix), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17967
Clone ID Related Accession (Same CDS sequence)	NM_140015.3 , NM_168306.2
Accession Version	NM_168306.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pixie, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168306.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCGCA GAAAGGAGAA CGAGGAGGTG GACAAGCAGA CGCGTATTGC CATCGTCAGC 
GATGACAAGT GCAAGCCCAA ACGGTGTCGG CAGGAGTGCA AGAAGACCTG CCCAGTGGTG 
CGCATGGGCA AGCTCTGCAT CGAGGTGACG CCCACGTCCA AGATAGCCTC GTTGTCCGAG 
GAGCTGTGCA TCGGTTGCGG TATTTGCGTC AAGAAATGCC CCTTCGAGGC CATCACAATC 
ATCAACTTGC CCAGCAATCT GGAGAAGCAC ACAACGCATC GATACAGCAA GAACTCCTTC 
AAGCTGCATC GTCTGCCCAT TCCGCGTCCC GGTGAAGTGC TCGGTCTTGT GGGACAGAAC 
GGTATTGGCA AGTCGACCGC TTTGAAGATC CTCGCGGGGA AACAAAAGCC GAACCTGGGG 
AAGTACGCCA ATCCGCCCGA CTGGACGGAG ATCCTTAGCT ACTTCCGCGG CTCCGAGCTG 
CAGAACTACT TCACCAAGAT CTTGGAGGAC AATCTGAAGG CGCTGGTCAA GCCGCAGTAT 
GTGGATCAGA TCCCCAAGGC GGTGCGTGGC GCTGTGGGCG ATCTTCTGGA TAAGAAGGAC 
GAGAGGGAGC TGCAGACTAA GATTTGTGAA ATGCTGGATC TATCGCACAT CCGTGATCGT 
GAGATCGCTC AGCTTTCTGG CGGAGAACTG CAACGCTTTG CCATCGCCAT GGTATGCATA 
CAGAATGCAG ACATCTTCAT GTTCGACGAG CCGTCCTCGT ATTTGGATGT TAAGCAGCGT 
CTGAACGCTG CCTTGACCAT TCGATCACTC CTGCATCCCA CCAAGTTTAT TATTGTGGTG 
GAGCACGATT TGTCTGTGCT GGATTACTTG TCTGACTTTA TATGCTGCCT TTACGGAGTT 
CCCGGCTGCT ACGGTGTGGT TACTATGCCT TTCTCTGTGC GCGAAGGCAT CAACATTTTC 
CTCGATGGCT TTGTGCCCAC CGAAAACATG CGATTCCGCA CTGAGTCACT TACTTTCAAA 
GTATCTGAGT CCGCTACGGA GGAGGAGATC AAGCGCATGA ACCACTACGT ATATCCCGCC 
ATGGTCAAGA CCCTGGGCAA GTTCGAGTTG ACGGTGGAAA AGGGTCACTT CAGCGACTCC 
GAGATCCTGG TGCTCCTGGG CGAGAACGGT ACAGGAAAGA CGACGTTCAT TCGCATGCTG 
GCCGGTAACC TCCAACCCGA TGGCGAGGTG GAACTGCCTA TGCTGAATAT TTCCTACAAG 
CCCCAGAAGA TTTCCCCGAA ATTCCAAAAT CATGTTCGCC ATTTATTGCA CGATAAGATT 
CGTGACGCCT ACGTGCATCC TCAGTTTATT GCCGATGTTA TGAAACCCAT GAAAATTGAG 
GAAATCATGG ACCAGGAGGT GCAGAACCTT TCCGGTGGTG AGTTGCAGCG AGTCGCCTTG 
GTCCTGTGTC TTGGCAAGCC AGCGGATGTC TACCTCATCG ACGAACCCTC TGCCTACTTG 
GATTCAGAGC AGCGTCTGGT GGCCGCCAAG GTTATCAAAC GTTACATTCT GCACGCCAAG 
AAAACTGGAT TTGTGGTGGA GCACGATTTC ATCATGGCCA CCTACCTCGC CGACCGTGTC 
ATCGTGATCG AGGGTCAGCC TTCAGTGAAG ACGACAGCCT TTTCGCCGCA GTCGCTGCTA 
AATGGCATGA ACCGCTTCCT CGAACTGCTT GGTATTACGT TCCGTCGCGA TCCCAACAAC 
TTCAGACCCC GCATTAACAA AAACAATTCG GTCAAGGACA CAGAACAGAA ACGCTCCGGC 
CAGTTCTTCT TCTTGGAGGA CGAAGCCTGC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729435.1
CDS	72..1907
Translation	MSRRKENEEVDKQTRIAIVSDDKCKPKRCRQECKKTCPVVRMGKLCIEVTPTSKIASLSEELCIGCGICVKKCPFEAITIINLPSNLEKHTTHRYSKNSFKLHRLPIPRPGEVLGLVGQNGIGKSTALKILAGKQKPNLGKYANPPDWTEILSYFRGSELQNYFTKILEDNLKALVKPQYVDQIPKAVRGAVGDLLDKKDERELQTKICEMLDLSHIRDREIAQLSGGELQRFAIAMVCIQNADIFMFDEPSSYLDVKQRLNAALTIRSLLHPTKFIIVVEHDLSVLDYLSDFICCLYGVPGCYGVVTMPFSVREGINIFLDGFVPTENMRFRTESLTFKVSESATEEEIKRMNHYVYPAMVKTLGKFELTVEKGHFSDSEILVLLGENGTGKTTFIRMLAGNLQPDGEVELPMLNISYKPQKISPKFQNHVRHLLHDKIRDAYVHPQFIADVMKPMKIEEIMDQEVQNLSGGELQRVALVLCLGKPADVYLIDEPSAYLDSEQRLVAAKVIKRYILHAKKTGFVVEHDFIMATYLADRVIVIEGQPSVKTTAFSPQSLLNGMNRFLELLGITFRRDPNNFRPRINKNNSVKDTEQKRSGQFFFLEDEACN

Target ORF information:

RefSeq Version	NM_168306.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pixie (pix), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168306.2

ORF Insert Sequence:

ATGTCGCGCA GAAAGGAGAA CGAGGAGGTG GACAAGCAGA CGCGTATTGC CATCGTCAGC 
GATGACAAGT GCAAGCCCAA ACGGTGTCGG CAGGAGTGCA AGAAGACCTG CCCAGTGGTG 
CGCATGGGCA AGCTCTGCAT CGAGGTGACG CCCACGTCCA AGATAGCCTC GTTGTCCGAG 
GAGCTGTGCA TCGGTTGCGG TATTTGCGTC AAGAAATGCC CCTTCGAGGC CATCACAATC 
ATCAACTTGC CCAGCAATCT GGAGAAGCAC ACAACGCATC GATACAGCAA GAACTCCTTC 
AAGCTGCATC GTCTGCCCAT TCCGCGTCCC GGTGAAGTGC TCGGTCTTGT GGGACAGAAC 
GGTATTGGCA AGTCGACCGC TTTGAAGATC CTCGCGGGGA AACAAAAGCC GAACCTGGGG 
AAGTACGCCA ATCCGCCCGA CTGGACGGAG ATCCTTAGCT ACTTCCGCGG CTCCGAGCTG 
CAGAACTACT TCACCAAGAT CTTGGAGGAC AATCTGAAGG CGCTGGTCAA GCCGCAGTAT 
GTGGATCAGA TCCCCAAGGC GGTGCGTGGC GCTGTGGGCG ATCTTCTGGA TAAGAAGGAC 
GAGAGGGAGC TGCAGACTAA GATTTGTGAA ATGCTGGATC TATCGCACAT CCGTGATCGT 
GAGATCGCTC AGCTTTCTGG CGGAGAACTG CAACGCTTTG CCATCGCCAT GGTATGCATA 
CAGAATGCAG ACATCTTCAT GTTCGACGAG CCGTCCTCGT ATTTGGATGT TAAGCAGCGT 
CTGAACGCTG CCTTGACCAT TCGATCACTC CTGCATCCCA CCAAGTTTAT TATTGTGGTG 
GAGCACGATT TGTCTGTGCT GGATTACTTG TCTGACTTTA TATGCTGCCT TTACGGAGTT 
CCCGGCTGCT ACGGTGTGGT TACTATGCCT TTCTCTGTGC GCGAAGGCAT CAACATTTTC 
CTCGATGGCT TTGTGCCCAC CGAAAACATG CGATTCCGCA CTGAGTCACT TACTTTCAAA 
GTATCTGAGT CCGCTACGGA GGAGGAGATC AAGCGCATGA ACCACTACGT ATATCCCGCC 
ATGGTCAAGA CCCTGGGCAA GTTCGAGTTG ACGGTGGAAA AGGGTCACTT CAGCGACTCC 
GAGATCCTGG TGCTCCTGGG CGAGAACGGT ACAGGAAAGA CGACGTTCAT TCGCATGCTG 
GCCGGTAACC TCCAACCCGA TGGCGAGGTG GAACTGCCTA TGCTGAATAT TTCCTACAAG 
CCCCAGAAGA TTTCCCCGAA ATTCCAAAAT CATGTTCGCC ATTTATTGCA CGATAAGATT 
CGTGACGCCT ACGTGCATCC TCAGTTTATT GCCGATGTTA TGAAACCCAT GAAAATTGAG 
GAAATCATGG ACCAGGAGGT GCAGAACCTT TCCGGTGGTG AGTTGCAGCG AGTCGCCTTG 
GTCCTGTGTC TTGGCAAGCC AGCGGATGTC TACCTCATCG ACGAACCCTC TGCCTACTTG 
GATTCAGAGC AGCGTCTGGT GGCCGCCAAG GTTATCAAAC GTTACATTCT GCACGCCAAG 
AAAACTGGAT TTGTGGTGGA GCACGATTTC ATCATGGCCA CCTACCTCGC CGACCGTGTC 
ATCGTGATCG AGGGTCAGCC TTCAGTGAAG ACGACAGCCT TTTCGCCGCA GTCGCTGCTA 
AATGGCATGA ACCGCTTCCT CGAACTGCTT GGTATTACGT TCCGTCGCGA TCCCAACAAC 
TTCAGACCCC GCATTAACAA AAACAATTCG GTCAAGGACA CAGAACAGAA ACGCTCCGGC 
CAGTTCTTCT TCTTGGAGGA CGAAGCCTGC AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

pix cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報