mfr cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mfr
Entrez Gene ID	39016
Full Name	misfire
Synonyms	CG5747,Dmel\CG5747,Otoferlin
General protein information	Preferred Names misfire Names CG5747 gene product from transcript CG5747-RC CG5747-PB CG5747-PC CG5747-PD CG5747-PE CG5747-PF CG5747-PI CG5747-PJ mfr-PB mfr-PC mfr-PD mfr-PE mfr-PF mfr-PI mfr-PJ otoferlin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66E3-66E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001144445.2	NP_001137917.1	misfire, isoform D
NM_001144449.2	NP_001137921.1	misfire, isoform J
NM_001144444.2	NP_001137916.1	misfire, isoform C
NM_001144448.2	NP_001137920.1	misfire, isoform I
NM_001144443.2	NP_001137915.1	misfire, isoform B
NM_001144446.2	NP_001137918.1	misfire, isoform E
NM_001144447.2	NP_001137919.1	misfire, isoform F

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FER1L6	XP_519944.2
Canis lupus familiaris (dog)	FER1L6	XP_539157.4
Bos taurus (cattle)	FER1L6	XP_002692673.2
Danio rerio (zebrafish)	fer1l6	XP_003199239.2
Xenopus tropicalis (tropical clawed frog)	fer1l6	XP_002941446.2
Mus musculus (house mouse)	Fer1l6	XP_006521672.1
Drosophila melanogaster (fruit fly)	mfr	NP_001137919.1
Rattus norvegicus (Norway rat)	Fer1l6	XP_006241747.1
Homo sapiens (human)	FER1L6	NP_001034201.2
	FER1L6	XP_002805528.1
Gallus gallus (chicken)	FER1L6	XP_003640913.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The mfr gene exhibits complex transcriptional and post-transcriptional regulation and functions in three developmental processes: sperm activation, egg patterning, and early embryogenesis
Title: Complex regulation and multiple developmental functions of misfire, the Drosophila melanogaster ferlin gene.

The following mfr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mfr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17951	NM_001144446.2 Latest version!	Drosophila melanogaster misfire (mfr), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa17956	NM_001144449.2 Latest version!	Drosophila melanogaster misfire (mfr), transcript variant J, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa17953	NM_001144443.2 Latest version!	Drosophila melanogaster misfire (mfr), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa17950	NM_001144444.2 Latest version!	Drosophila melanogaster misfire (mfr), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa17952	NM_001144447.2 Latest version!	Drosophila melanogaster misfire (mfr), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa17954	NM_001144445.2 Latest version!	Drosophila melanogaster misfire (mfr), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa17955	NM_001144448.2 Latest version!	Drosophila melanogaster misfire (mfr), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mfr ( NM_001144446.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137918.1 Drosophila melanogaster misfire (mfr), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa17951
Clone ID Related Accession (Same CDS sequence)	NM_001144446.2
Accession Version	NM_001144446.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	misfire, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001144446.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCTATG TTGGTCGTCT TCTAATGGAA CTTCGGTCTG AAATGCTAAC GGATTCCCAG 
CCCACACACA CGCTGTCCTC CAAGACGGTG CCAGGTTTGG ATGAGACCCG TTTCTGGCAT 
GACGATGTCT ATCTGATTGA GTTTCTTCCA CTAATGGGTC ACCACATCCT GACCTCGGGT 
ACGGCATACA AGATCTCGGC GAGATTAGCC GAACACACCT CGAACGTGCT GGAGGGTCAA 
CTAAGGACAC CTTTAGATCG ATTCAAGTCG GCAATGAACT CAAAGAATTT CCGGCGGGAG 
GCACCTTTTC AGTCCTGCAT GTTTCGTGTC CGTTTGCCGG ACAATCGAGC CAAGTACGAA 
AGTGATTTCT TCATGCTGGA TATAGTCAAT TATATGCGTT CAGAACTGGA CACCTTCAAG 
CTCTTTCAAC GCAAATATCC ACTCCAGGAT GATGAGCAGG CCAAATGCCT GAAGGTCATT 
GTGAACAATA TTTTGACCAG AATCAAGGAG GGTCTAGATG CCCACCGTTT CGACCACGAC 
GTGCAACCAC AGCGCACCAC CTGGGATATC AATCGGCAGC TGTACCTCCT GGACTATTTC 
GCCAAGTTGC CACTGGAACT TCAAAATCTT AAACAGAAAC TACGCAGCTG CTTTCTGGAA 
CACCTAGACA CTTCCATTGG CGATGTGGTT AATGAGCTAC AGCGTTTGGT AGCTGAAATC 
GGATCCCTTT CCAGTATGGC ACGCACCCAG GACGAGTGGC CAGAATTGGT GCTTTCCTTA 
AGTGCAAGCG GTAAAGATGT GGGTGTTTGC CGATTGAACG CCAAACTCTT TTTGAATCTA 
CGAAATCAGG ATGCGGATCA GGATCAGGAG ACCCTTTGTT GGCGTCTAAA GAGCTTTTCC 
TTCAAGTCCG CGGGCTGTTC GCACACCTGC CCCAATTGTG GTTGCACCAC GGCCATAGTA 
TCAGGATGTT TATCCATTGT TGTGGAACGG GAGCGAAAAC GATTTCTTGC GGTTGTCAAT 
AAAGAGTGGG CCAACATTGA GCCCATGTTG TGGCAGCTGA ATCCTGAACA GACACACTTC 
CTCTGTCACG TTTACATACA CCAAGCCAAA GTTCGTCCTG GTGGTGAAAA AAAGAACATA 
TGTGACTCAC ATCTGAGAGT CCTGTTTGCC GACCAGGCAT GTGAAACGTA TACGTCTCCC 
GGTACCCTAT CGCCAATTTG GAATGCTGTG ATCACATTTA ACTGGGTGTC ACTACCAGGA 
GGCATTGGTC TCTATCTAAA GAACCCTCCT CTACTTTCCT TGGAATTCTA TAATTCGGAA 
CGCAGTCTAC CAGAAGACCT TGTGGGCATG GGAAGCGTGG CCATGAGTGT TATTAGCGAG 
GATCGGGCAA GTGATTCCAA TTGGGAGGAT GCAGGAGTAT TCGGTTGCTC AAAGAATCCG 
TTGCGAAAAC TACAGAGATT GAAGTACCTG ACACCACCTC CTCTCAAATG GGTGCCAATT 
GCTAGAAAAG GAACTCTACA AGCAGAGGTT TTGATGTCAG CCGAGATCAT TCAAATCTCC 
ATGGAATCTC TGACCACTGG TCAGAATGAT AAAAAGGAAC CACAACTTGC AACGGGCATT 
CCAATGGCCA TCAGGCCAAC TATGCAGAAT TTTGTCTTAG AGGTTTTCTT CGTGGGTCTA 
CGCAATTACT CCAAGAGCAG CATGAGTTCT GCTGGTAAGC GGAAAATCAA TTTAATGATG 
GGTGATCTGG TCCTGTCCAG TGGACCCTCC ACAGCACGCA TCCGGAACAG CATAAACTTT 
TTGGTGGCCT ACGCATCTGG AGTGGTTGTA AGTTCATATC GTAGAAAAGT AAGGTCCCAC 
CTATATAGTA CCCCGATCAG AGTTTGCCAG ATCAACAGGA TTACTGGCCG GCGATCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137918.1
CDS	1433..3352
Translation	MIYVGRLLMELRSEMLTDSQPTHTLSSKTVPGLDETRFWHDDVYLIEFLPLMGHHILTSGTAYKISARLAEHTSNVLEGQLRTPLDRFKSAMNSKNFRREAPFQSCMFRVRLPDNRAKYESDFFMLDIVNYMRSELDTFKLFQRKYPLQDDEQAKCLKVIVNNILTRIKEGLDAHRFDHDVQPQRTTWDINRQLYLLDYFAKLPLELQNLKQKLRSCFLEHLDTSIGDVVNELQRLVAEIGSLSSMARTQDEWPELVLSLSASGKDVGVCRLNAKLFLNLRNQDADQDQETLCWRLKSFSFKSAGCSHTCPNCGCTTAIVSGCLSIVVERERKRFLAVVNKEWANIEPMLWQLNPEQTHFLCHVYIHQAKVRPGGEKKNICDSHLRVLFADQACETYTSPGTLSPIWNAVITFNWVSLPGGIGLYLKNPPLLSLEFYNSERSLPEDLVGMGSVAMSVISEDRASDSNWEDAGVFGCSKNPLRKLQRLKYLTPPPLKWVPIARKGTLQAEVLMSAEIIQISMESLTTGQNDKKEPQLATGIPMAIRPTMQNFVLEVFFVGLRNYSKSSMSSAGKRKINLMMGDLVLSSGPSTARIRNSINFLVAYASGVVVSSYRRKVRSHLYSTPIRVCQINRITGRRS

Target ORF information:

RefSeq Version	NM_001144446.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster misfire (mfr), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144446.2

ORF Insert Sequence:

ATGATCTATG TTGGTCGTCT TCTAATGGAA CTTCGGTCTG AAATGCTAAC GGATTCCCAG 
CCCACACACA CGCTGTCCTC CAAGACGGTG CCAGGTTTGG ATGAGACCCG TTTCTGGCAT 
GACGATGTCT ATCTGATTGA GTTTCTTCCA CTAATGGGTC ACCACATCCT GACCTCGGGT 
ACGGCATACA AGATCTCGGC GAGATTAGCC GAACACACCT CGAACGTGCT GGAGGGTCAA 
CTAAGGACAC CTTTAGATCG ATTCAAGTCG GCAATGAACT CAAAGAATTT CCGGCGGGAG 
GCACCTTTTC AGTCCTGCAT GTTTCGTGTC CGTTTGCCGG ACAATCGAGC CAAGTACGAA 
AGTGATTTCT TCATGCTGGA TATAGTCAAT TATATGCGTT CAGAACTGGA CACCTTCAAG 
CTCTTTCAAC GCAAATATCC ACTCCAGGAT GATGAGCAGG CCAAATGCCT GAAGGTCATT 
GTGAACAATA TTTTGACCAG AATCAAGGAG GGTCTAGATG CCCACCGTTT CGACCACGAC 
GTGCAACCAC AGCGCACCAC CTGGGATATC AATCGGCAGC TGTACCTCCT GGACTATTTC 
GCCAAGTTGC CACTGGAACT TCAAAATCTT AAACAGAAAC TACGCAGCTG CTTTCTGGAA 
CACCTAGACA CTTCCATTGG CGATGTGGTT AATGAGCTAC AGCGTTTGGT AGCTGAAATC 
GGATCCCTTT CCAGTATGGC ACGCACCCAG GACGAGTGGC CAGAATTGGT GCTTTCCTTA 
AGTGCAAGCG GTAAAGATGT GGGTGTTTGC CGATTGAACG CCAAACTCTT TTTGAATCTA 
CGAAATCAGG ATGCGGATCA GGATCAGGAG ACCCTTTGTT GGCGTCTAAA GAGCTTTTCC 
TTCAAGTCCG CGGGCTGTTC GCACACCTGC CCCAATTGTG GTTGCACCAC GGCCATAGTA 
TCAGGATGTT TATCCATTGT TGTGGAACGG GAGCGAAAAC GATTTCTTGC GGTTGTCAAT 
AAAGAGTGGG CCAACATTGA GCCCATGTTG TGGCAGCTGA ATCCTGAACA GACACACTTC 
CTCTGTCACG TTTACATACA CCAAGCCAAA GTTCGTCCTG GTGGTGAAAA AAAGAACATA 
TGTGACTCAC ATCTGAGAGT CCTGTTTGCC GACCAGGCAT GTGAAACGTA TACGTCTCCC 
GGTACCCTAT CGCCAATTTG GAATGCTGTG ATCACATTTA ACTGGGTGTC ACTACCAGGA 
GGCATTGGTC TCTATCTAAA GAACCCTCCT CTACTTTCCT TGGAATTCTA TAATTCGGAA 
CGCAGTCTAC CAGAAGACCT TGTGGGCATG GGAAGCGTGG CCATGAGTGT TATTAGCGAG 
GATCGGGCAA GTGATTCCAA TTGGGAGGAT GCAGGAGTAT TCGGTTGCTC AAAGAATCCG 
TTGCGAAAAC TACAGAGATT GAAGTACCTG ACACCACCTC CTCTCAAATG GGTGCCAATT 
GCTAGAAAAG GAACTCTACA AGCAGAGGTT TTGATGTCAG CCGAGATCAT TCAAATCTCC 
ATGGAATCTC TGACCACTGG TCAGAATGAT AAAAAGGAAC CACAACTTGC AACGGGCATT 
CCAATGGCCA TCAGGCCAAC TATGCAGAAT TTTGTCTTAG AGGTTTTCTT CGTGGGTCTA 
CGCAATTACT CCAAGAGCAG CATGAGTTCT GCTGGTAAGC GGAAAATCAA TTTAATGATG 
GGTGATCTGG TCCTGTCCAG TGGACCCTCC ACAGCACGCA TCCGGAACAG CATAAACTTT 
TTGGTGGCCT ACGCATCTGG AGTGGTTGTA AGTTCATATC GTAGAAAAGT AAGGTCCCAC 
CTATATAGTA CCCCGATCAG AGTTTGCCAG ATCAACAGGA TTACTGGCCG GCGATCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mfr ( NM_001144449.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137921.1 Drosophila melanogaster misfire (mfr), transcript variant J, mRNA.

Price & Availability↑

CloneID	OFa17956
Clone ID Related Accession (Same CDS sequence)	NM_001144449.2
Accession Version	NM_001144449.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	misfire, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001144449.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGACTGC TCTTCGCGTT CGGTCTGCGT CCAGCCCCCT ATGCCAATCG ACACGCTTTA 
AGGTTCCACG ATGATTTGGA TGACCACGAA TTGGTCGAGG AGCGAGAGTT CACCTGGTGG 
ACTAAGTTCT ACAACTCGAT GTATTGGAGT GCGGCCGAAA TGTGCCACGA ACACAAGCAC 
CGCCTGGTTA TCTACTACGA AGAGCTGGAG AAGCAGGCCC AGTTTGGATA CCTCCAGGAT 
TGGGCGGTTC CCGTTCAGTT GGTTCATGGC GTGAAGTTTA AAAAGCATGG TCCACCCAAA 
GAAGATATCT ATGCCACCTT AAAGCTGCAA CTCAAGTTGA CCCCTTGTCA GTGCCCGGTT 
CTTGAGGATC CCGGAGGTGG TGGCGACATG GTTCGCAACA TGTCAAATCC CATACATCCT 
CGACATCAGT CCACACTACA GTCGCTGGGT GAGACCGTAA AACTATTGGT ACGTGTTTAT 
GTAGTCCAGG GGGTTCAGAT GCGTCCTCGA GATGTGAAAG GTGACTCCGA TTGCTATGTG 
AAGCTGTTCC TGGGCGGCAA GACGTTCTCC GATAGAGCCC ATTTCTCACC CAACCACAGT 
AATCCAGTTT TCGGTCGTCT ATTTGAAATG GAGGCTACGC TGCCAGGAGA TCATATGCTG 
CAAGTCATGG TATATGATCA TGACAAGATC AAGGACGAGG TGATCGGTCA GACAAACATC 
GATCTGGAGG ATCGCTGGCG AACTAGGCAT CGTGCCACAG TGGGTTTGGC CAATGAGTAC 
ACAAAGAGTG GCTATAATCA TTGGCACGAC GTTAAGTTGC CCTCGGAAAT TCTCATAGAT 
CTGTGCCAGA AAAGAGGCAT TCAGGCCCCT TACTATTATG GTAATGTAAT TGAGGTGGAC 
GGAATGCTTT TTGGGGACGA AACTTTGATC TCAAAAGATG AGGAGCTGCA GGAGAGACTT 
AGTTTAGCCG TGCTTAAGAA TATGGATAAG TTGCCATCCT TTGGTTACAA ATTGGTTCCC 
GAACATGTGG AAACCCGCTC CCTTTGCCGC GATGATTTTC CAAATGTCGA ACAGGGCAAA 
CTACAACTGT GGATAGAGCT GTTCGAGGCG AACATCTATG TGCCCAGTCC CATCGATATA 
ACCCCTGTGC CGCCCGCGGA TTTCGAGGTG CGTGTTGTGG TTAAGAATCT GGTCGGGATT 
CAGGCGGGCG ACAAAAACAT TTTTGGCAAA CTAATGAGCG ACGTTTACGT GATAGGATGG 
TGCGAGGATG AGGACAAGCG CCAATCGACC GATATCCATT ACCGCTCATT TGCAGGAGAT 
GCGGCATTTA ATTGGCGCAT GGTCTTCGGC CTCAAGTATT CCCCCAACGA GGACCTGATG 
GTAATCCGGC GAAAGGCGGG GCTGCTGGAG GAAATCGAGT TCAAGAAGCC GCCTATTATC 
TATCTGCAGG TGTGGGACAA GGATGTGTTA ACCAAGGATG AGTATCTGGC CGCACTGGAG 
CTCAATCTCT CCAACATGTA CGCACCGTAT CCAGCTGCCC AGTTATGTAA GCCATATCCC 
AAGAAGAGGA AGCGCATCAA TCTCTTCAAG CGCAAGGTCA TCAACGGCTG GTTTCCGCTG 
CAAAGTAATG CACATCCATC GCAACGCAGC CAGGCCGCAG TTCAAAATGG CAAAATGCAG 
CTGAGCATCG AAGTCTGCAC GGATGTGGAG GCCGTCAACC GGCCGGCTGG TCGTGGACAG 
GATCCACCGA TGGCCTTGGA GCCGCCTTAC AGACCGGACA GCTCGTTCAA TCCACTAACG 
CATCCTTGCA AATCCTTTCA GCACATCCTC TGGCCAGTCA TTCGAAAGTA TGTCCTGTGG 
ACTTTGCTCA TATTGATCGT TATCCTGGGA TTGGTGCTCT TCTTTTCCAA TCTGCCCGCC 
AAACTCATGA CCATTCCACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137921.1
CDS	3037..4983
Translation	MRLLFAFGLRPAPYANRHALRFHDDLDDHELVEEREFTWWTKFYNSMYWSAAEMCHEHKHRLVIYYEELEKQAQFGYLQDWAVPVQLVHGVKFKKHGPPKEDIYATLKLQLKLTPCQCPVLEDPGGGGDMVRNMSNPIHPRHQSTLQSLGETVKLLVRVYVVQGVQMRPRDVKGDSDCYVKLFLGGKTFSDRAHFSPNHSNPVFGRLFEMEATLPGDHMLQVMVYDHDKIKDEVIGQTNIDLEDRWRTRHRATVGLANEYTKSGYNHWHDVKLPSEILIDLCQKRGIQAPYYYGNVIEVDGMLFGDETLISKDEELQERLSLAVLKNMDKLPSFGYKLVPEHVETRSLCRDDFPNVEQGKLQLWIELFEANIYVPSPIDITPVPPADFEVRVVVKNLVGIQAGDKNIFGKLMSDVYVIGWCEDEDKRQSTDIHYRSFAGDAAFNWRMVFGLKYSPNEDLMVIRRKAGLLEEIEFKKPPIIYLQVWDKDVLTKDEYLAALELNLSNMYAPYPAAQLCKPYPKKRKRINLFKRKVINGWFPLQSNAHPSQRSQAAVQNGKMQLSIEVCTDVEAVNRPAGRGQDPPMALEPPYRPDSSFNPLTHPCKSFQHILWPVIRKYVLWTLLILIVILGLVLFFSNLPAKLMTIPLE

Target ORF information:

RefSeq Version	NM_001144449.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster misfire (mfr), transcript variant J, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144449.2

ORF Insert Sequence:

ATGCGACTGC TCTTCGCGTT CGGTCTGCGT CCAGCCCCCT ATGCCAATCG ACACGCTTTA 
AGGTTCCACG ATGATTTGGA TGACCACGAA TTGGTCGAGG AGCGAGAGTT CACCTGGTGG 
ACTAAGTTCT ACAACTCGAT GTATTGGAGT GCGGCCGAAA TGTGCCACGA ACACAAGCAC 
CGCCTGGTTA TCTACTACGA AGAGCTGGAG AAGCAGGCCC AGTTTGGATA CCTCCAGGAT 
TGGGCGGTTC CCGTTCAGTT GGTTCATGGC GTGAAGTTTA AAAAGCATGG TCCACCCAAA 
GAAGATATCT ATGCCACCTT AAAGCTGCAA CTCAAGTTGA CCCCTTGTCA GTGCCCGGTT 
CTTGAGGATC CCGGAGGTGG TGGCGACATG GTTCGCAACA TGTCAAATCC CATACATCCT 
CGACATCAGT CCACACTACA GTCGCTGGGT GAGACCGTAA AACTATTGGT ACGTGTTTAT 
GTAGTCCAGG GGGTTCAGAT GCGTCCTCGA GATGTGAAAG GTGACTCCGA TTGCTATGTG 
AAGCTGTTCC TGGGCGGCAA GACGTTCTCC GATAGAGCCC ATTTCTCACC CAACCACAGT 
AATCCAGTTT TCGGTCGTCT ATTTGAAATG GAGGCTACGC TGCCAGGAGA TCATATGCTG 
CAAGTCATGG TATATGATCA TGACAAGATC AAGGACGAGG TGATCGGTCA GACAAACATC 
GATCTGGAGG ATCGCTGGCG AACTAGGCAT CGTGCCACAG TGGGTTTGGC CAATGAGTAC 
ACAAAGAGTG GCTATAATCA TTGGCACGAC GTTAAGTTGC CCTCGGAAAT TCTCATAGAT 
CTGTGCCAGA AAAGAGGCAT TCAGGCCCCT TACTATTATG GTAATGTAAT TGAGGTGGAC 
GGAATGCTTT TTGGGGACGA AACTTTGATC TCAAAAGATG AGGAGCTGCA GGAGAGACTT 
AGTTTAGCCG TGCTTAAGAA TATGGATAAG TTGCCATCCT TTGGTTACAA ATTGGTTCCC 
GAACATGTGG AAACCCGCTC CCTTTGCCGC GATGATTTTC CAAATGTCGA ACAGGGCAAA 
CTACAACTGT GGATAGAGCT GTTCGAGGCG AACATCTATG TGCCCAGTCC CATCGATATA 
ACCCCTGTGC CGCCCGCGGA TTTCGAGGTG CGTGTTGTGG TTAAGAATCT GGTCGGGATT 
CAGGCGGGCG ACAAAAACAT TTTTGGCAAA CTAATGAGCG ACGTTTACGT GATAGGATGG 
TGCGAGGATG AGGACAAGCG CCAATCGACC GATATCCATT ACCGCTCATT TGCAGGAGAT 
GCGGCATTTA ATTGGCGCAT GGTCTTCGGC CTCAAGTATT CCCCCAACGA GGACCTGATG 
GTAATCCGGC GAAAGGCGGG GCTGCTGGAG GAAATCGAGT TCAAGAAGCC GCCTATTATC 
TATCTGCAGG TGTGGGACAA GGATGTGTTA ACCAAGGATG AGTATCTGGC CGCACTGGAG 
CTCAATCTCT CCAACATGTA CGCACCGTAT CCAGCTGCCC AGTTATGTAA GCCATATCCC 
AAGAAGAGGA AGCGCATCAA TCTCTTCAAG CGCAAGGTCA TCAACGGCTG GTTTCCGCTG 
CAAAGTAATG CACATCCATC GCAACGCAGC CAGGCCGCAG TTCAAAATGG CAAAATGCAG 
CTGAGCATCG AAGTCTGCAC GGATGTGGAG GCCGTCAACC GGCCGGCTGG TCGTGGACAG 
GATCCACCGA TGGCCTTGGA GCCGCCTTAC AGACCGGACA GCTCGTTCAA TCCACTAACG 
CATCCTTGCA AATCCTTTCA GCACATCCTC TGGCCAGTCA TTCGAAAGTA TGTCCTGTGG 
ACTTTGCTCA TATTGATCGT TATCCTGGGA TTGGTGCTCT TCTTTTCCAA TCTGCCCGCC 
AAACTCATGA CCATTCCACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mfr ( NM_001144443.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137915.1 Drosophila melanogaster misfire (mfr), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17953
Clone ID Related Accession (Same CDS sequence)	NM_001144443.2
Accession Version	NM_001144443.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3138bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	misfire, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001144443.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCACGCA CCCAGGACGA GTGGCCAGAA TTGGTGCTTT CCTTAAGTGC AAGCGGTAAA 
GATGTGGGTG TTTGCCGATT GAACGCCAAA CTCTTTTTGA ATCTACGAAA TCAGGATGCG 
GATCAGGATC AGGAGACCCT TTGTTGGCGT CTAAAGAGCT TTTCCTTCAA GTCCGCGGGC 
TGTTCGCACA CCTGCCCCAA TTGTGGTTGC ACCACGGCCA TAGTATCAGG ATGTTTATCC 
ATTGTTGTGG AACGGGAGCG AAAACGATTT CTTGCGGTTG TCAATAAAGA GTGGGCCAAC 
ATTGAGCCCA TGTTGTGGCA GCTGAATCCT GAACAGACAC ACTTCCTCTG TCACGTTTAC 
ATACACCAAG CCAAAGTTCG TCCTGGTGGT GAAAAAAAGA ACATATGTGA CTCACATCTG 
AGAGTCCTGT TTGCCGACCA GGCATGTGAA ACGTATACGT CTCCCGGTAC CCTATCGCCA 
ATTTGGAATG CTGTGATCAC ATTTAACTGG GTGTCACTAC CAGGAGGCAT TGGTCTCTAT 
CTAAAGAACC CTCCTCTACT TTCCTTGGAA TTCTATAATT CGGAACGCAG TCTACCAGAA 
GACCTTGTGG GCATGGGAAG CGTGGCCATG AGTGTTATTA GCGAGGATCG GGCAAGTGAT 
TCCAATTGGG AGGATGCAGG AGTATTCGGT TGCTCAAAGA ATCCGTTGCG AAAACTACAG 
AGATTGAAGT ACCTGACACC ACCTCCTCTC AAATGGGTGC CAATTGCTAG AAAAGGAACT 
CTACAAGCAG AGGTTTTGAT GTCAGCCGAG ATCATTCAAA TCTCCATGGA ATCTCTGACC 
ACTGGTCAGA ATGATAAAAA GGAACCACAA CTTGCAACGG GCATTCCAAT GGCCATCAGG 
CCAACTATGC AGAATTTTGT CTTAGAGGTT TTCTTCGTGG GTCTACGCAA TTACTCCAAG 
AGCAGCATGA GTTCTGCTGG TAAGCGGAAA ATCAATTTAA TGATGGGTGA TCTGGTCCTG 
TCCAGTGGAC CCTCCACAGC ACGCATCCGG AACAGCATAA ACTTTTTGGT GGCCTACGCA 
TCTGGAGTGG TTAGTTTGCC AGATCAACAG GATTACTGGC CGGCGATCAT AGCCACCGAT 
GTCCTTCTCT CCGGATTTAG CAGCGAGTCC ATTCTGGGTG CAGCTCTCAT ACCCAATTCA 
TCAAATTTCC TGCAACGCGA CAGGACCCTC AAGTGTGTGT TCAAAAAGGA ACAAGAGGCA 
GCAGAAGCTT CCACCACGGT TAGTGTGGAT GAAGATGATG AGGATGAGGA AGAGGAACTG 
GAGTGGGAAG AGGAGGAGAC CAAACCCTCC AGGATTAGCA GCTGGTGGAT GCGACTGCTC 
TTCGCGTTCG GTCTGCGTCC AGCCCCCTAT GCCAATCGAC ACGCTTTAAG GTTCCACGAT 
GATTTGGATG ACCACGAATT GGTCGAGGAG CGAGAGTTCA CCTGGTGGAC TAAGTTCTAC 
AACTCGATGT ATTGGAGTGC GGCCGAAATG TGCCACGAAC ACAAGCACCG CCTGGTTATC 
TACTACGAAG AGCTGGAGAA GCAGGCCCAG TTTGGATACC TCCAGGATTG GGCGGTTCCC 
GTTCAGTTGG TTCATGGCGT GAAGTTTAAA AAGCATGGTC CACCCAAAGA AGATATCTAT 
GCCACCTTAA AGCTGCAACT CAAGTTGACC CCTTGTCAGT GCCCGGTTCT TGAGGATCCC 
GGAGGTGGTG GCGACATGGT TCGCAACATG TCAAATCCCA TACATCCTCG ACATCAGTCC 
ACACTACAGT CGCTGGGTGA GACCGTAAAA CTATTGGTAC GTGTTTATGT AGTCCAGGGG 
GTTCAGATGC GTCCTCGAGA TGTGAAAGGT GACTCCGATT GCTATGTGAA GCTGTTCCTG 
GGCGGCAAGA CGTTCTCCGA TAGAGCCCAT TTCTCACCCA ACCACAGTAA TCCAGTTTTC 
GGTCGTCTAT TTGAAATGGA GGCTACGCTG CCAGGAGATC ATATGCTGCA AGTCATGGTA 
TATGATCATG ACAAGATCAA GGACGAGGTG ATCGGTCAGA CAAACATCGA TCTGGAGGAT 
CGCTGGCGAA CTAGGCATCG TGCCACAGTG GGTTTGGCCA ATGAGTACAC AAAGAGTGGC 
TATAATCATT GGCACGACGT TAAGTTGCCC TCGGAAATTC TCATAGATCT GTGCCAGAAA 
AGAGGCATTC AGGCCCCTTA CTATTATGGT AATGTAATTG AGGTGGACGG AATGCTTTTT 
GGGGACGAAA CTTTGATCTC AAAAGATGAG GAGCTGCAGG AGAGACTTAG TTTAGCCGTG 
CTTAAGAATA TGGATAAGTT GCCATCCTTT GGTTACAAAT TGGTTCCCGA ACATGTGGAA 
ACCCGCTCCC TTTGCCGCGA TGATTTTCCA AATGTCGAAC AGGGCAAACT ACAACTGTGG 
ATAGAGCTGT TCGAGGCGAA CATCTATGTG CCCAGTCCCA TCGATATAAC CCCTGTGCCG 
CCCGCGGATT TCGAGGTGCG TGTTGTGGTT AAGAATCTGG TCGGGATTCA GGCGGGCGAC 
AAAAACATTT TTGGCAAACT AATGAGCGAC GTTTACGTGA TAGGATGGTG CGAGGATGAG 
GACAAGCGCC AATCGACCGA TATCCATTAC CGCTCATTTG CAGGAGATGC GGCATTTAAT 
TGGCGCATGG TCTTCGGCCT CAAGTATTCC CCCAACGAGG ACCTGATGGT AATCCGGCGA 
AAGGCGGGGC TGCTGGAGGA AATCGAGTTC AAGAAGCCGC CTATTATCTA TCTGCAGGTG 
TGGGACAAGG ATGTGTTAAC CAAGGATGAG TATCTGGCCG CACTGGAGCT CAATCTCTCC 
AACATGTACG CACCGTATCC AGCTGCCCAG TTATGTAAGC CATATCCCAA GAAGAGGAAG 
CGCATCAATC TCTTCAAGCG CAAGGTCATC AACGGCTGGT TTCCGCTGCA AAGTAATGCA 
CATCCATCGC AACGCAGCCA GGCCGCAGTT CAAAATGGCA AAATGCAGCT GAGCATCGAA 
GTCTGCACGG ATGTGGAGGC CGTCAACCGG CCGGCTGGTC GTGGACAGGA TCCACCGATG 
GCCTTGGAGC CGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137915.1
CDS	1733..4870
Translation	MARTQDEWPELVLSLSASGKDVGVCRLNAKLFLNLRNQDADQDQETLCWRLKSFSFKSAGCSHTCPNCGCTTAIVSGCLSIVVERERKRFLAVVNKEWANIEPMLWQLNPEQTHFLCHVYIHQAKVRPGGEKKNICDSHLRVLFADQACETYTSPGTLSPIWNAVITFNWVSLPGGIGLYLKNPPLLSLEFYNSERSLPEDLVGMGSVAMSVISEDRASDSNWEDAGVFGCSKNPLRKLQRLKYLTPPPLKWVPIARKGTLQAEVLMSAEIIQISMESLTTGQNDKKEPQLATGIPMAIRPTMQNFVLEVFFVGLRNYSKSSMSSAGKRKINLMMGDLVLSSGPSTARIRNSINFLVAYASGVVSLPDQQDYWPAIIATDVLLSGFSSESILGAALIPNSSNFLQRDRTLKCVFKKEQEAAEASTTVSVDEDDEDEEEELEWEEEETKPSRISSWWMRLLFAFGLRPAPYANRHALRFHDDLDDHELVEEREFTWWTKFYNSMYWSAAEMCHEHKHRLVIYYEELEKQAQFGYLQDWAVPVQLVHGVKFKKHGPPKEDIYATLKLQLKLTPCQCPVLEDPGGGGDMVRNMSNPIHPRHQSTLQSLGETVKLLVRVYVVQGVQMRPRDVKGDSDCYVKLFLGGKTFSDRAHFSPNHSNPVFGRLFEMEATLPGDHMLQVMVYDHDKIKDEVIGQTNIDLEDRWRTRHRATVGLANEYTKSGYNHWHDVKLPSEILIDLCQKRGIQAPYYYGNVIEVDGMLFGDETLISKDEELQERLSLAVLKNMDKLPSFGYKLVPEHVETRSLCRDDFPNVEQGKLQLWIELFEANIYVPSPIDITPVPPADFEVRVVVKNLVGIQAGDKNIFGKLMSDVYVIGWCEDEDKRQSTDIHYRSFAGDAAFNWRMVFGLKYSPNEDLMVIRRKAGLLEEIEFKKPPIIYLQVWDKDVLTKDEYLAALELNLSNMYAPYPAAQLCKPYPKKRKRINLFKRKVINGWFPLQSNAHPSQRSQAAVQNGKMQLSIEVCTDVEAVNRPAGRGQDPPMALEPP

Target ORF information:

RefSeq Version	NM_001144443.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster misfire (mfr), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144443.2

ORF Insert Sequence:

ATGGCACGCA CCCAGGACGA GTGGCCAGAA TTGGTGCTTT CCTTAAGTGC AAGCGGTAAA 
GATGTGGGTG TTTGCCGATT GAACGCCAAA CTCTTTTTGA ATCTACGAAA TCAGGATGCG 
GATCAGGATC AGGAGACCCT TTGTTGGCGT CTAAAGAGCT TTTCCTTCAA GTCCGCGGGC 
TGTTCGCACA CCTGCCCCAA TTGTGGTTGC ACCACGGCCA TAGTATCAGG ATGTTTATCC 
ATTGTTGTGG AACGGGAGCG AAAACGATTT CTTGCGGTTG TCAATAAAGA GTGGGCCAAC 
ATTGAGCCCA TGTTGTGGCA GCTGAATCCT GAACAGACAC ACTTCCTCTG TCACGTTTAC 
ATACACCAAG CCAAAGTTCG TCCTGGTGGT GAAAAAAAGA ACATATGTGA CTCACATCTG 
AGAGTCCTGT TTGCCGACCA GGCATGTGAA ACGTATACGT CTCCCGGTAC CCTATCGCCA 
ATTTGGAATG CTGTGATCAC ATTTAACTGG GTGTCACTAC CAGGAGGCAT TGGTCTCTAT 
CTAAAGAACC CTCCTCTACT TTCCTTGGAA TTCTATAATT CGGAACGCAG TCTACCAGAA 
GACCTTGTGG GCATGGGAAG CGTGGCCATG AGTGTTATTA GCGAGGATCG GGCAAGTGAT 
TCCAATTGGG AGGATGCAGG AGTATTCGGT TGCTCAAAGA ATCCGTTGCG AAAACTACAG 
AGATTGAAGT ACCTGACACC ACCTCCTCTC AAATGGGTGC CAATTGCTAG AAAAGGAACT 
CTACAAGCAG AGGTTTTGAT GTCAGCCGAG ATCATTCAAA TCTCCATGGA ATCTCTGACC 
ACTGGTCAGA ATGATAAAAA GGAACCACAA CTTGCAACGG GCATTCCAAT GGCCATCAGG 
CCAACTATGC AGAATTTTGT CTTAGAGGTT TTCTTCGTGG GTCTACGCAA TTACTCCAAG 
AGCAGCATGA GTTCTGCTGG TAAGCGGAAA ATCAATTTAA TGATGGGTGA TCTGGTCCTG 
TCCAGTGGAC CCTCCACAGC ACGCATCCGG AACAGCATAA ACTTTTTGGT GGCCTACGCA 
TCTGGAGTGG TTAGTTTGCC AGATCAACAG GATTACTGGC CGGCGATCAT AGCCACCGAT 
GTCCTTCTCT CCGGATTTAG CAGCGAGTCC ATTCTGGGTG CAGCTCTCAT ACCCAATTCA 
TCAAATTTCC TGCAACGCGA CAGGACCCTC AAGTGTGTGT TCAAAAAGGA ACAAGAGGCA 
GCAGAAGCTT CCACCACGGT TAGTGTGGAT GAAGATGATG AGGATGAGGA AGAGGAACTG 
GAGTGGGAAG AGGAGGAGAC CAAACCCTCC AGGATTAGCA GCTGGTGGAT GCGACTGCTC 
TTCGCGTTCG GTCTGCGTCC AGCCCCCTAT GCCAATCGAC ACGCTTTAAG GTTCCACGAT 
GATTTGGATG ACCACGAATT GGTCGAGGAG CGAGAGTTCA CCTGGTGGAC TAAGTTCTAC 
AACTCGATGT ATTGGAGTGC GGCCGAAATG TGCCACGAAC ACAAGCACCG CCTGGTTATC 
TACTACGAAG AGCTGGAGAA GCAGGCCCAG TTTGGATACC TCCAGGATTG GGCGGTTCCC 
GTTCAGTTGG TTCATGGCGT GAAGTTTAAA AAGCATGGTC CACCCAAAGA AGATATCTAT 
GCCACCTTAA AGCTGCAACT CAAGTTGACC CCTTGTCAGT GCCCGGTTCT TGAGGATCCC 
GGAGGTGGTG GCGACATGGT TCGCAACATG TCAAATCCCA TACATCCTCG ACATCAGTCC 
ACACTACAGT CGCTGGGTGA GACCGTAAAA CTATTGGTAC GTGTTTATGT AGTCCAGGGG 
GTTCAGATGC GTCCTCGAGA TGTGAAAGGT GACTCCGATT GCTATGTGAA GCTGTTCCTG 
GGCGGCAAGA CGTTCTCCGA TAGAGCCCAT TTCTCACCCA ACCACAGTAA TCCAGTTTTC 
GGTCGTCTAT TTGAAATGGA GGCTACGCTG CCAGGAGATC ATATGCTGCA AGTCATGGTA 
TATGATCATG ACAAGATCAA GGACGAGGTG ATCGGTCAGA CAAACATCGA TCTGGAGGAT 
CGCTGGCGAA CTAGGCATCG TGCCACAGTG GGTTTGGCCA ATGAGTACAC AAAGAGTGGC 
TATAATCATT GGCACGACGT TAAGTTGCCC TCGGAAATTC TCATAGATCT GTGCCAGAAA 
AGAGGCATTC AGGCCCCTTA CTATTATGGT AATGTAATTG AGGTGGACGG AATGCTTTTT 
GGGGACGAAA CTTTGATCTC AAAAGATGAG GAGCTGCAGG AGAGACTTAG TTTAGCCGTG 
CTTAAGAATA TGGATAAGTT GCCATCCTTT GGTTACAAAT TGGTTCCCGA ACATGTGGAA 
ACCCGCTCCC TTTGCCGCGA TGATTTTCCA AATGTCGAAC AGGGCAAACT ACAACTGTGG 
ATAGAGCTGT TCGAGGCGAA CATCTATGTG CCCAGTCCCA TCGATATAAC CCCTGTGCCG 
CCCGCGGATT TCGAGGTGCG TGTTGTGGTT AAGAATCTGG TCGGGATTCA GGCGGGCGAC 
AAAAACATTT TTGGCAAACT AATGAGCGAC GTTTACGTGA TAGGATGGTG CGAGGATGAG 
GACAAGCGCC AATCGACCGA TATCCATTAC CGCTCATTTG CAGGAGATGC GGCATTTAAT 
TGGCGCATGG TCTTCGGCCT CAAGTATTCC CCCAACGAGG ACCTGATGGT AATCCGGCGA 
AAGGCGGGGC TGCTGGAGGA AATCGAGTTC AAGAAGCCGC CTATTATCTA TCTGCAGGTG 
TGGGACAAGG ATGTGTTAAC CAAGGATGAG TATCTGGCCG CACTGGAGCT CAATCTCTCC 
AACATGTACG CACCGTATCC AGCTGCCCAG TTATGTAAGC CATATCCCAA GAAGAGGAAG 
CGCATCAATC TCTTCAAGCG CAAGGTCATC AACGGCTGGT TTCCGCTGCA AAGTAATGCA 
CATCCATCGC AACGCAGCCA GGCCGCAGTT CAAAATGGCA AAATGCAGCT GAGCATCGAA 
GTCTGCACGG ATGTGGAGGC CGTCAACCGG CCGGCTGGTC GTGGACAGGA TCCACCGATG 
GCCTTGGAGC CGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mfr ( NM_001144444.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137916.1 Drosophila melanogaster misfire (mfr), transcript variant C, mRNA.

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CloneID	OFa17950
Clone ID Related Accession (Same CDS sequence)	NM_001144444.2
Accession Version	NM_001144444.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	misfire, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001144444.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTCCGT TTCTAAAGTA CAGATTCCAT CCTTTCAAGG GCAAAACGCC GGTGGTCAAG 
AACACGACGA CGCCCGTGTG GAACTACGAG ATCAACTTTG CCTGGATGTA TCCGTCGGTG 
GCCCAGCGAT TCCTCATCCT AATTTTTGCC CATGAACATC TGCAATGGAA ATGCGTGGCT 
GAGTTTGAAT TGTGTCTTGA GGAGATCGCG TTTAATGGAA CTCCTTCTCT GGGACCCACC 
TACTTGCATC TCTACGATCC TGTCAATCCT ATGATCTATG TTGGTCGTCT TCTAATGGAA 
CTTCGGTCTG AAATGCTAAC GGATTCCCAG CCCACACACA CGCTGTCCTC CAAGACGGTG 
CCAGGTTTGG ATGAGACCCG TTTCTGGCAT GACGATGTCT ATCTGATTGA GTTTCTTCCA 
CTAATGGGTC ACCACATCCT GACCTCGGGT ACGGCATACA AGATCTCGGC GAGATTAGCC 
GAACACACCT CGAACGTGCT GGAGGGTCAA CTAAGGACAC CTTTAGATCG ATTCAAGTCG 
GCAATGAACT CAAAGAATTT CCGGCGGGAG GCACCTTTTC AGTCCTGCAT GTTTCGTGTC 
CGTTTGCCGG ACAATCGAGC CAAGTACGAA AGTGATTTCT TCATGCTGGA TATAGTCAAT 
TATATGCGTT CAGAACTGGA CACCTTCAAG CTCTTTCAAC GCAAATATCC ACTCCAGGAT 
GATGAGCAGG CCAAATGCCT GAAGGTCATT GTGAACAATA TTTTGACCAG AATCAAGGAG 
GGTCTAGATG CCCACCGTTT CGACCACGAC GTGCAACCAC AGCGCACCAC CTGGGATATC 
AATCGGCAGC TGTACCTCCT GGACTATTTC GCCAAGTTGC CACTGGAACT TCAAAATCTT 
AAACAGAAAC TACGCAGCTG CTTTCTGGAA CACCTAGACA CTTCCATTGG CGATGTGGTT 
AATGAGCTAC AGCGTTTGGT AGCTGAAATC GGATCCCTTT CCAGTATGGC ACGCACCCAG 
GACGAGTGGC CAGAATTGGT GCTTTCCTTA AGTGCAAGCG GTAAAGATGT GGGTGTTTGC 
CGATTGAACG CCAAACTCTT TTTGAATCTA CGAAATCAGG ATGCGGATCA GGATCAGGAG 
ACCCTTTGTT GGCGTCTAAA GAGCTTTTCC TTCAAGTCCG CGGGCTGTTC GCACACCTGC 
CCCAATTGTG GTTGCACCAC GGCCATAGTA TCAGGATGTT TATCCATTGT TGTGGAACGG 
GAGCGAAAAC GATTTCTTGC GGTTGTCAAT AAAGAGTGGG CCAACATTGA GCCCATGTTG 
TGGCAGCTGA ATCCTGAACA GACACACTTC CTCTGTCACG TTTACATACA CCAAGCCAAA 
GTTCGTCCTG GTGGTGAAAA AAAGAACATA TGTGACTCAC ATCTGAGAGT CCTGTTTGCC 
GACCAGGCAT GTGAAACGTA TACGTCTCCC GGTACCCTAT CGCCAATTTG GAATGCTGTG 
ATCACATTTA ACTGGGTGTC ACTACCAGGA GGCATTGGTC TCTATCTAAA GAACCCTCCT 
CTACTTTCCT TGGAATTCTA TAATTCGGAA CGCAGTCTAC CAGAAGACCT TGTGGGCATG 
GGAAGCGTGG CCATGAGTGT TATTAGCGAG GATCGGGCAA GTGATTCCAA TTGGGAGGAT 
GCAGGAGTAT TCGGTTGCTC AAAGAATCCG TTGCGAAAAC TACAGAGATT GAAGTACCTG 
ACACCACCTC CTCTCAAATG GGTGCCAATT GCTAGAAAAG GAACTCTACA AGCAGAGGTT 
TTGATGTCAG CCGAGATCAT TCAAATCTCC ATGGAATCTC TGACCACTGG TCAGAATGAT 
AAAAAGGAAC CACAACTTGC AACGGGCATT CCAATGGCCA TCAGGCCAAC TATGCAGAAT 
TTTGTCTTAG AGGTTTTCTT CGTGGGTCTA CGCAATTACT CCAAGAGCAG CATGAGTTCT 
GCTGGTAAGC GGAAAATCAA TTTAATGATG GGTGATCTGG TCCTGTCCAG TGGACCCTCC 
ACAGCACGCA TCCGGAACAG CATAAACTTT TTGGTGGCCT ACGCATCTGG AGTGGTTAGT 
TTGCCAGATC AACAGGATTA CTGGCCGGCG ATCATAGCCA CCGATGTCCT TCTCTCCGGA 
TTTAGCAGCG AGTCCATTCT GGGTGCAGCT CTCATACCCA ATTCATCAAA TTTCCTGCAA 
CGCGACAGGA CCCTCAAGTG TGTGTTCAAA AAGGAACAAG AGGCAGCAGA AGCTTCCACC 
ACGGTTAGTG TGGATGAAGA TGATGAGGAT GAGGAAGAGG AACTGGAGTG GGAAGAGGAG 
GAGACCAAAC CCTCCAGGAT TAGCAGCTGG TGGATGCGAC TGCTCTTCGC GTTCGGTCTG 
CGTCCAGCCC CCTATGCCAA TCGACACGCT TTAAGGTTCC ACGATGATTT GGATGACCAC 
GAATTGGTCG AGGAGCGAGA GTTCACCTGG TGGACTAAGT TCTACAACTC GATGTATTGG 
AGTGCGGCCG AAATGTGCCA CGAACACAAG CACCGCCTGG TTATCTACTA CGAAGAGCTG 
GAGAAGCAGG CCCAGTTTGG ATACCTCCAG GATTGGGCGG TTCCCGTTCA GTTGGTTCAT 
GGCGTGAAGT TTAAAAAGCA TGGTCCACCC AAAGAAGATA TCTATGCCAC CTTAAAGCTG 
CAACTCAAGT TGACCCCTTG TCAGTGCCCG GTTCTTGAGG ATCCCGGAGG TGGTGGCGAC 
ATGGTTCGCA ACATGTCAAA TCCCATACAT CCTCGACATC AGTCCACACT ACAGTCGCTG 
GGTGAGACCG TAAAACTATT GGTACGTGTT TATGTAGTCC AGGGGGTTCA GATGCGTCCT 
CGAGATGTGA AAGGTGACTC CGATTGCTAT GTGAAGCTGT TCCTGGGCGG CAAGACGTTC 
TCCGATAGAG CCCATTTCTC ACCCAACCAC AGTAATCCAG TTTTCGGTCG TCTATTTGAA 
ATGGAGGCTA CGCTGCCAGG AGATCATATG CTGCAAGTCA TGGTATATGA TCATGACAAG 
ATCAAGGACG AGGTGATCGG TCAGACAAAC ATCGATCTGG AGGATCGCTG GCGAACTAGG 
CATCGTGCCA CAGTGGGTTT GGCCAATGAG TACACAAAGA GTGGCTATAA TCATTGGCAC 
GACGTTAAGT TGCCCTCGGA AATTCTCATA GATCTGTGCC AGAAAAGAGG CATTCAGGCC 
CCTTACTATT ATGGTAATGT AATTGAGGTG GACGGAATGC TTTTTGGGGA CGAAACTTTG 
ATCTCAAAAG ATGAGGAGCT GCAGGAGAGA CTTAGTTTAG CCGTGCTTAA GAATATGGAT 
AAGTTGCCAT CCTTTGGTTA CAAATTGGTT CCCGAACATG TGGAAACCCG CTCCCTTTGC 
CGCGATGATT TTCCAAATGT CGAACAGGGC AAACTACAAC TGTGGATAGA GCTGTTCGAG 
GCGAACATCT ATGTGCCCAG TCCCATCGAT ATAACCCCTG TGCCGCCCGC GGATTTCGAG 
GTGCGTGTTG TGGTTAAGAA TCTGGTCGGG ATTCAGGCGG GCGACAAAAA CATTTTTGGC 
AAACTAATGA GCGACGTTTA CGTGATAGGA TGGTGCGAGG ATGAGGACAA GCGCCAATCG 
ACCGATATCC ATTACCGCTC ATTTGCAGGA GATGCGGCAT TTAATTGGCG CATGGTCTTC 
GGCCTCAAGT ATTCCCCCAA CGAGGACCTG ATGGTAATCC GGCGAAAGGC GGGGCTGCTG 
GAGGAAATCG AGTTCAAGAA GCCGCCTATT ATCTATCTGC AGGTGTGGGA CAAGGATGTG 
TTAACCAAGG ATGAGTATCT GGCCGCACTG GAGCTCAATC TCTCCAACAT GTACGCACCG 
TATCCAGCTG CCCAGTTATG TAAGCCATAT CCCAAGAAGA GGAAGCGCAT CAATCTCTTC 
AAGCGCAAGG TCATCAACGG CTGGTTTCCG CTGCAAAGTA ATGCACATCC ATCGCAACGC 
AGCCAGGCCG CAGTTCAAAA TGGCAAAATG CAGCTGAGCA TCGAAGTCTG CACGGATGTG 
GAGGCCGTCA ACCGGCCGGC TGGTCGTGGA CAGGATCCAC CGATGGCCTT GGAGCCGCCT 
TACACATCCT CTGGCCAGTC ATTCGAAAGT ATGTCCTGTG GACTTTGCTC ATATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137916.1
CDS	1217..5413
Translation	MFPFLKYRFHPFKGKTPVVKNTTTPVWNYEINFAWMYPSVAQRFLILIFAHEHLQWKCVAEFELCLEEIAFNGTPSLGPTYLHLYDPVNPMIYVGRLLMELRSEMLTDSQPTHTLSSKTVPGLDETRFWHDDVYLIEFLPLMGHHILTSGTAYKISARLAEHTSNVLEGQLRTPLDRFKSAMNSKNFRREAPFQSCMFRVRLPDNRAKYESDFFMLDIVNYMRSELDTFKLFQRKYPLQDDEQAKCLKVIVNNILTRIKEGLDAHRFDHDVQPQRTTWDINRQLYLLDYFAKLPLELQNLKQKLRSCFLEHLDTSIGDVVNELQRLVAEIGSLSSMARTQDEWPELVLSLSASGKDVGVCRLNAKLFLNLRNQDADQDQETLCWRLKSFSFKSAGCSHTCPNCGCTTAIVSGCLSIVVERERKRFLAVVNKEWANIEPMLWQLNPEQTHFLCHVYIHQAKVRPGGEKKNICDSHLRVLFADQACETYTSPGTLSPIWNAVITFNWVSLPGGIGLYLKNPPLLSLEFYNSERSLPEDLVGMGSVAMSVISEDRASDSNWEDAGVFGCSKNPLRKLQRLKYLTPPPLKWVPIARKGTLQAEVLMSAEIIQISMESLTTGQNDKKEPQLATGIPMAIRPTMQNFVLEVFFVGLRNYSKSSMSSAGKRKINLMMGDLVLSSGPSTARIRNSINFLVAYASGVVSLPDQQDYWPAIIATDVLLSGFSSESILGAALIPNSSNFLQRDRTLKCVFKKEQEAAEASTTVSVDEDDEDEEEELEWEEEETKPSRISSWWMRLLFAFGLRPAPYANRHALRFHDDLDDHELVEEREFTWWTKFYNSMYWSAAEMCHEHKHRLVIYYEELEKQAQFGYLQDWAVPVQLVHGVKFKKHGPPKEDIYATLKLQLKLTPCQCPVLEDPGGGGDMVRNMSNPIHPRHQSTLQSLGETVKLLVRVYVVQGVQMRPRDVKGDSDCYVKLFLGGKTFSDRAHFSPNHSNPVFGRLFEMEATLPGDHMLQVMVYDHDKIKDEVIGQTNIDLEDRWRTRHRATVGLANEYTKSGYNHWHDVKLPSEILIDLCQKRGIQAPYYYGNVIEVDGMLFGDETLISKDEELQERLSLAVLKNMDKLPSFGYKLVPEHVETRSLCRDDFPNVEQGKLQLWIELFEANIYVPSPIDITPVPPADFEVRVVVKNLVGIQAGDKNIFGKLMSDVYVIGWCEDEDKRQSTDIHYRSFAGDAAFNWRMVFGLKYSPNEDLMVIRRKAGLLEEIEFKKPPIIYLQVWDKDVLTKDEYLAALELNLSNMYAPYPAAQLCKPYPKKRKRINLFKRKVINGWFPLQSNAHPSQRSQAAVQNGKMQLSIEVCTDVEAVNRPAGRGQDPPMALEPPYTSSGQSFESMSCGLCSY

Target ORF information:

RefSeq Version	NM_001144444.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster misfire (mfr), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144444.2

ORF Insert Sequence:

ATGTTTCCGT TTCTAAAGTA CAGATTCCAT CCTTTCAAGG GCAAAACGCC GGTGGTCAAG 
AACACGACGA CGCCCGTGTG GAACTACGAG ATCAACTTTG CCTGGATGTA TCCGTCGGTG 
GCCCAGCGAT TCCTCATCCT AATTTTTGCC CATGAACATC TGCAATGGAA ATGCGTGGCT 
GAGTTTGAAT TGTGTCTTGA GGAGATCGCG TTTAATGGAA CTCCTTCTCT GGGACCCACC 
TACTTGCATC TCTACGATCC TGTCAATCCT ATGATCTATG TTGGTCGTCT TCTAATGGAA 
CTTCGGTCTG AAATGCTAAC GGATTCCCAG CCCACACACA CGCTGTCCTC CAAGACGGTG 
CCAGGTTTGG ATGAGACCCG TTTCTGGCAT GACGATGTCT ATCTGATTGA GTTTCTTCCA 
CTAATGGGTC ACCACATCCT GACCTCGGGT ACGGCATACA AGATCTCGGC GAGATTAGCC 
GAACACACCT CGAACGTGCT GGAGGGTCAA CTAAGGACAC CTTTAGATCG ATTCAAGTCG 
GCAATGAACT CAAAGAATTT CCGGCGGGAG GCACCTTTTC AGTCCTGCAT GTTTCGTGTC 
CGTTTGCCGG ACAATCGAGC CAAGTACGAA AGTGATTTCT TCATGCTGGA TATAGTCAAT 
TATATGCGTT CAGAACTGGA CACCTTCAAG CTCTTTCAAC GCAAATATCC ACTCCAGGAT 
GATGAGCAGG CCAAATGCCT GAAGGTCATT GTGAACAATA TTTTGACCAG AATCAAGGAG 
GGTCTAGATG CCCACCGTTT CGACCACGAC GTGCAACCAC AGCGCACCAC CTGGGATATC 
AATCGGCAGC TGTACCTCCT GGACTATTTC GCCAAGTTGC CACTGGAACT TCAAAATCTT 
AAACAGAAAC TACGCAGCTG CTTTCTGGAA CACCTAGACA CTTCCATTGG CGATGTGGTT 
AATGAGCTAC AGCGTTTGGT AGCTGAAATC GGATCCCTTT CCAGTATGGC ACGCACCCAG 
GACGAGTGGC CAGAATTGGT GCTTTCCTTA AGTGCAAGCG GTAAAGATGT GGGTGTTTGC 
CGATTGAACG CCAAACTCTT TTTGAATCTA CGAAATCAGG ATGCGGATCA GGATCAGGAG 
ACCCTTTGTT GGCGTCTAAA GAGCTTTTCC TTCAAGTCCG CGGGCTGTTC GCACACCTGC 
CCCAATTGTG GTTGCACCAC GGCCATAGTA TCAGGATGTT TATCCATTGT TGTGGAACGG 
GAGCGAAAAC GATTTCTTGC GGTTGTCAAT AAAGAGTGGG CCAACATTGA GCCCATGTTG 
TGGCAGCTGA ATCCTGAACA GACACACTTC CTCTGTCACG TTTACATACA CCAAGCCAAA 
GTTCGTCCTG GTGGTGAAAA AAAGAACATA TGTGACTCAC ATCTGAGAGT CCTGTTTGCC 
GACCAGGCAT GTGAAACGTA TACGTCTCCC GGTACCCTAT CGCCAATTTG GAATGCTGTG 
ATCACATTTA ACTGGGTGTC ACTACCAGGA GGCATTGGTC TCTATCTAAA GAACCCTCCT 
CTACTTTCCT TGGAATTCTA TAATTCGGAA CGCAGTCTAC CAGAAGACCT TGTGGGCATG 
GGAAGCGTGG CCATGAGTGT TATTAGCGAG GATCGGGCAA GTGATTCCAA TTGGGAGGAT 
GCAGGAGTAT TCGGTTGCTC AAAGAATCCG TTGCGAAAAC TACAGAGATT GAAGTACCTG 
ACACCACCTC CTCTCAAATG GGTGCCAATT GCTAGAAAAG GAACTCTACA AGCAGAGGTT 
TTGATGTCAG CCGAGATCAT TCAAATCTCC ATGGAATCTC TGACCACTGG TCAGAATGAT 
AAAAAGGAAC CACAACTTGC AACGGGCATT CCAATGGCCA TCAGGCCAAC TATGCAGAAT 
TTTGTCTTAG AGGTTTTCTT CGTGGGTCTA CGCAATTACT CCAAGAGCAG CATGAGTTCT 
GCTGGTAAGC GGAAAATCAA TTTAATGATG GGTGATCTGG TCCTGTCCAG TGGACCCTCC 
ACAGCACGCA TCCGGAACAG CATAAACTTT TTGGTGGCCT ACGCATCTGG AGTGGTTAGT 
TTGCCAGATC AACAGGATTA CTGGCCGGCG ATCATAGCCA CCGATGTCCT TCTCTCCGGA 
TTTAGCAGCG AGTCCATTCT GGGTGCAGCT CTCATACCCA ATTCATCAAA TTTCCTGCAA 
CGCGACAGGA CCCTCAAGTG TGTGTTCAAA AAGGAACAAG AGGCAGCAGA AGCTTCCACC 
ACGGTTAGTG TGGATGAAGA TGATGAGGAT GAGGAAGAGG AACTGGAGTG GGAAGAGGAG 
GAGACCAAAC CCTCCAGGAT TAGCAGCTGG TGGATGCGAC TGCTCTTCGC GTTCGGTCTG 
CGTCCAGCCC CCTATGCCAA TCGACACGCT TTAAGGTTCC ACGATGATTT GGATGACCAC 
GAATTGGTCG AGGAGCGAGA GTTCACCTGG TGGACTAAGT TCTACAACTC GATGTATTGG 
AGTGCGGCCG AAATGTGCCA CGAACACAAG CACCGCCTGG TTATCTACTA CGAAGAGCTG 
GAGAAGCAGG CCCAGTTTGG ATACCTCCAG GATTGGGCGG TTCCCGTTCA GTTGGTTCAT 
GGCGTGAAGT TTAAAAAGCA TGGTCCACCC AAAGAAGATA TCTATGCCAC CTTAAAGCTG 
CAACTCAAGT TGACCCCTTG TCAGTGCCCG GTTCTTGAGG ATCCCGGAGG TGGTGGCGAC 
ATGGTTCGCA ACATGTCAAA TCCCATACAT CCTCGACATC AGTCCACACT ACAGTCGCTG 
GGTGAGACCG TAAAACTATT GGTACGTGTT TATGTAGTCC AGGGGGTTCA GATGCGTCCT 
CGAGATGTGA AAGGTGACTC CGATTGCTAT GTGAAGCTGT TCCTGGGCGG CAAGACGTTC 
TCCGATAGAG CCCATTTCTC ACCCAACCAC AGTAATCCAG TTTTCGGTCG TCTATTTGAA 
ATGGAGGCTA CGCTGCCAGG AGATCATATG CTGCAAGTCA TGGTATATGA TCATGACAAG 
ATCAAGGACG AGGTGATCGG TCAGACAAAC ATCGATCTGG AGGATCGCTG GCGAACTAGG 
CATCGTGCCA CAGTGGGTTT GGCCAATGAG TACACAAAGA GTGGCTATAA TCATTGGCAC 
GACGTTAAGT TGCCCTCGGA AATTCTCATA GATCTGTGCC AGAAAAGAGG CATTCAGGCC 
CCTTACTATT ATGGTAATGT AATTGAGGTG GACGGAATGC TTTTTGGGGA CGAAACTTTG 
ATCTCAAAAG ATGAGGAGCT GCAGGAGAGA CTTAGTTTAG CCGTGCTTAA GAATATGGAT 
AAGTTGCCAT CCTTTGGTTA CAAATTGGTT CCCGAACATG TGGAAACCCG CTCCCTTTGC 
CGCGATGATT TTCCAAATGT CGAACAGGGC AAACTACAAC TGTGGATAGA GCTGTTCGAG 
GCGAACATCT ATGTGCCCAG TCCCATCGAT ATAACCCCTG TGCCGCCCGC GGATTTCGAG 
GTGCGTGTTG TGGTTAAGAA TCTGGTCGGG ATTCAGGCGG GCGACAAAAA CATTTTTGGC 
AAACTAATGA GCGACGTTTA CGTGATAGGA TGGTGCGAGG ATGAGGACAA GCGCCAATCG 
ACCGATATCC ATTACCGCTC ATTTGCAGGA GATGCGGCAT TTAATTGGCG CATGGTCTTC 
GGCCTCAAGT ATTCCCCCAA CGAGGACCTG ATGGTAATCC GGCGAAAGGC GGGGCTGCTG 
GAGGAAATCG AGTTCAAGAA GCCGCCTATT ATCTATCTGC AGGTGTGGGA CAAGGATGTG 
TTAACCAAGG ATGAGTATCT GGCCGCACTG GAGCTCAATC TCTCCAACAT GTACGCACCG 
TATCCAGCTG CCCAGTTATG TAAGCCATAT CCCAAGAAGA GGAAGCGCAT CAATCTCTTC 
AAGCGCAAGG TCATCAACGG CTGGTTTCCG CTGCAAAGTA ATGCACATCC ATCGCAACGC 
AGCCAGGCCG CAGTTCAAAA TGGCAAAATG CAGCTGAGCA TCGAAGTCTG CACGGATGTG 
GAGGCCGTCA ACCGGCCGGC TGGTCGTGGA CAGGATCCAC CGATGGCCTT GGAGCCGCCT 
TACACATCCT CTGGCCAGTC ATTCGAAAGT ATGTCCTGTG GACTTTGCTC ATATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mfr ( NM_001144447.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137919.1 Drosophila melanogaster misfire (mfr), transcript variant F, mRNA.

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CloneID	OFa17952
Clone ID Related Accession (Same CDS sequence)	NM_001144447.2
Accession Version	NM_001144447.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	misfire, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001144447.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTTACA TGGACGACTG TTTCGCAGGT GCTCCGCAGG AGTTTCTCAT CTGCATCACG 
GTGCACAAGG CTGCCCAAGT GGGAGTGACC ACCGGCGAGC TGTACGTGCG GATTAGCTTG 
GACAAAATGA CGAAGAGCAC CAAATCCTTT CCTAATACAG AAAATCCCTT CTTCAATGAG 
TACTTCGTTT TTGAGTTCCA TTGTACGTTA ACCGAACTCC TGCGCTTAAC GATCCTTTTT 
GAGCTAAAGA AACACATGAC GTATAAGAAA AATGTGGTAG TGGGTGAACT ACTGGTTGAT 
CTACATAGTG TGTGGAACCA GTCCAATCAT GGCTACTTCA AGAAGTGGGG TCGCCTGGAA 
GCTCCTATTG GAGAAAACCA GACCCTAGAG AATGCCGTTG CACCGCACGG CTATTTGCAA 
CTGGATCTGG CCATCGTATC CCAGCACAGT CCGGTGTCTT ATGCCCTCGG ATCTGAGGAG 
CAGGACACAC AAAATCTGAA CAAGTGGACG GTTGATCATG ATTTTGATGA CATCGAAAAG 
AATCTACTGA CCAACGTGAA CATCTTCGCC CCAAGTAATA TTCGATACTT CGTCGCCTTT 
TACCGGGGTT ACTTCATCAA GCAGAGCAAC TACATGATTC AAGTATCGTT TGCCGGCTTC 
AATGGCAAAA CGCCGGTGGT CAAGAACACG ACGACGCCCG TGTGGAACTA CGAGATCAAC 
TTTGCCTGGA TGTATCCGTC GGTGGCCCAG CGATTCCTCA TCCTAATTTT TGCCCATGAA 
CATCTGCAAT GGAAATGCGT GGCTGAGTTT GAATTGTGTC TTGAGGAGAT CGCGTTTAAT 
GGAACTCCTT CTCTGGGACC CACCTACTTG CATCTCTACG ATCCTGTCAA TCCTATGATC 
TATGTTGGTC GTCTTCTAAT GGAACTTCGG TCTGAAATGC TAACGGATTC CCAGCCCACA 
CACACGCTGT CCTCCAAGAC GGTGCCAGGT TTGGATGAGA CCCGTTTCTG GCATGACGAT 
GTCTATCTGA TTGAGTTTCT TCCACTAATG GGTCACCACA TCCTGACCTC GGGTACGGCA 
TACAAGATCT CGGCGAGATT AGCCGAACAC ACCTCGAACG TGCTGGAGGG TCAACTAAGG 
ACACCTTTAG ATCGATTCAA GTCGGCAATG AACTCAAAGA ATTTCCGGCG GGAGGCACCT 
TTTCAGTCCT GCATGTTTCG TGTCCGTTTG CCGGACAATC GAGCCAAGTA CGAAAGTGAT 
TTCTTCATGC TGGATATAGT CAATTATATG CGTTCAGAAC TGGACACCTT CAAGCTCTTT 
CAACGCAAAT ATCCACTCCA GGATGATGAG CAGGCCAAAT GCCTGAAGGT CATTGTGAAC 
AATATTTTGA CCAGAATCAA GGAGGGTCTA GATGCCCACC GTTTCGACCA CGACGTGCAA 
CCACAGCGCA CCACCTGGGA TATCAATCGG CAGCTGTACC TCCTGGACTA TTTCGCCAAG 
TTGCCACTGG AACTTCAAAA TCTTAAACAG AAACTACGCA GCTGCTTTCT GGAACACCTA 
GACACTTCCA TTGGCGATGT GGTTAATGAG CTACAGCGTT TGGTAGCTGA AATCGGATCC 
CTTTCCAGTA TGGCACGCAC CCAGGACGAG TGGCCAGAAT TGGTGCTTTC CTTAAGTGCA 
AGCGGTAAAG ATGTGGGTGT TTGCCGATTG AACGCCAAAC TCTTTTTGAA TCTACGAAAT 
CAGGATGCGG ATCAGGATCA GGAGACCCTT TGTTGGCGTC TAAAGAGCTT TTCCTTCAAG 
TCCGCGGGCT GTTCGCACAC CTGCCCCAAT TGTGGTTGCA CCACGGCCAT AGTATCAGGA 
TGTTTATCCA TTGTTGTGGA ACGGGAGCGA AAACGATTTC TTGCGGTTGT CAATAAAGAG 
TGGGCCAACA TTGAGCCCAT GTTGTGGCAG CTGAATCCTG AACAGACACA CTTCCTCTGT 
CACGTTTACA TACACCAAGC CAAAGTTCGT CCTGGTGGTG AAAAAAAGAA CATATGTGAC 
TCACATCTGA GAGTCCTGTT TGCCGACCAG GCATGTGAAA CGTATACGTC TCCCGGTACC 
CTATCGCCAA TTTGGAATGC TGTGATCACA TTTAACTGGG TGTCACTACC AGGAGGCATT 
GGTCTCTATC TAAAGAACCC TCCTCTACTT TCCTTGGAAT TCTATAATTC GGAACGCAGT 
CTACCAGAAG ACCTTGTGGG CATGGGAAGC GTGGCCATGA GTGTTATTAG CGAGGATCGG 
GCAAGTGATT CCAATTGGGA GGATGCAGGA GTATTCGGTT GCTCAAAGAA TCCGTTGCGA 
AAACTACAGA GATTGAAGTA CCTGACACCA CCTCCTCTCA AATGGGTGCC AATTGCTAGA 
AAAGGAACTC TACAAGCAGA GGTTTTGATG TCAGCCGAGA TCATTCAAAT CTCCATGGAA 
TCTCTGACCA CTGGTCAGAA TGATAAAAAG GAACCACAAC TTGCAACGGG CATTCCAATG 
GCCATCAGGC CAACTATGCA GAATTTTGTC TTAGAGGTTT TCTTCGTGGG TCTACGCAAT 
TACTCCAAGA GCAGCATGAG TTCTGCTGGT AAGCGGAAAA TCAATTTAAT GATGGGTGAT 
CTGGTCCTGT CCAGTGGACC CTCCACAGCA CGCATCCGGA ACAGCATAAA CTTTTTGGTG 
GCCTACGCAT CTGGAGTGGT TAGTTTGCCA GATCAACAGG ATTACTGGCC GGCGATCATA 
GCCACCGATG TCCTTCTCTC CGGATTTAGC AGCGAGTCCA TTCTGGGTGC AGCTCTCATA 
CCCAATTCAT CAAATTTCCT GCAACGCGAC AGGACCCTCA AGTGTGTGTT CAAAAAGGAA 
CAAGAGGCAG CAGAAGCTTC CACCACGGTT AGTGTGGATG AAGATGATGA GGATGAGGAA 
GAGGAACTGG AGTGGGAAGA GGAGGAGACC AAACCCTCCA GGATTAGCAG CTGGTGGATG 
CGACTGCTCT TCGCGTTCGG TCTGCGTCCA GCCCCCTATG CCAATCGACA CGCTTTAAGG 
TTCCACGATG ATTTGGATGA CCACGAATTG GTCGAGGAGC GAGAGTTCAC CTGGTGGACT 
AAGTTCTACA ACTCGATGTA TTGGAGTGCG GCCGAAATGT GCCACGAACA CAAGCACCGC 
CTGGTTATCT ACTACGAAGA GCTGGAGAAG CAGGCCCAGT TTGGATACCT CCAGGATTGG 
GCGGTTCCCG TTCAGTTGGT TCATGGCGTG AAGTTTAAAA AGCATGGTCC ACCCAAAGAA 
GATATCTATG CCACCTTAAA GCTGCAACTC AAGTTGACCC CTTGTCAGTG CCCGGTTCTT 
GAGGATCCCG GAGGTGGTGG CGACATGGTT CGCAACATGT CAAATCCCAT ACATCCTCGA 
CATCAGTCCA CACTACAGTC GCTGGGTGAG ACCGTAAAAC TATTGGTACG TGTTTATGTA 
GTCCAGGGGG TTCAGATGCG TCCTCGAGAT GTGAAAGGTG ACTCCGATTG CTATGTGAAG 
CTGTTCCTGG GCGGCAAGAC GTTCTCCGAT AGAGCCCATT TCTCACCCAA CCACAGTAAT 
CCAGTTTTCG GTCGTCTATT TGAAATGGAG GCTACGCTGC CAGGAGATCA TATGCTGCAA 
GTCATGGTAT ATGATCATGA CAAGATCAAG GACGAGGTGA TCGGTCAGAC AAACATCGAT 
CTGGAGGATC GCTGGCGAAC TAGGCATCGT GCCACAGTGG GTTTGGCCAA TGAGTACACA 
AAGAGTGGCT ATAATCATTG GCACGACGTT AAGTTGCCCT CGGAAATTCT CATAGATCTG 
TGCCAGAAAA GAGGCATTCA GGCCCCTTAC TATTATGGTA ATGTAATTGA GGTGGACGGA 
ATGCTTTTTG GGGACGAAAC TTTGATCTCA AAAGATGAGG AGCTGCAGGA GAGACTTAGT 
TTAGCCGTGC TTAAGAATAT GGATAAGTTG CCATCCTTTG GTTACAAATT GGTTCCCGAA 
CATGTGGAAA CCCGCTCCCT TTGCCGCGAT GATTTTCCAA ATGTCGAACA GGGCAAACTA 
CAACTGTGGA TAGAGCTGTT CGAGGCGAAC ATCTATGTGC CCAGTCCCAT CGATATAACC 
CCTGTGCCGC CCGCGGATTT CGAGGTGCGT GTTGTGGTTA AGAATCTGGT CGGGATTCAG 
GCGGGCGACA AAAACATTTT TGGCAAACTA ATGAGCGACG TTTACGTGAT AGGATGGTGC 
GAGGATGAGG ACAAGCGCCA ATCGACCGAT ATCCATTACC GCTCATTTGC AGGAGATGCG 
GCATTTAATT GGCGCATGGT CTTCGGCCTC AAGTATTCCC CCAACGAGGA CCTGATGGTA 
ATCCGGCGAA AGGCGGGGCT GCTGGAGGAA ATCGAGTTCA AGAAGCCGCC TATTATCTAT 
CTGCAGGTGT GGGACAAGGA TGTGTTAACC AAGGATGAGT ATCTGGCCGC ACTGGAGCTC 
AATCTCTCCA ACATGTACGC ACCGTATCCA GCTGCCCAGT TATGTAAGCC ATATCCCAAG 
AAGAGGAAGC GCATCAATCT CTTCAAGCGC AAGGTCATCA ACGGCTGGTT TCCGCTGCAA 
AGTAATGCAC ATCCATCGCA ACGCAGCCAG GCCGCAGTTC AAAATGGCAA AATGCAGCTG 
AGCATCGAAG TCTGCACGGA TGTGGAGGCC GTCAACCGGC CGGCTGGTCG TGGACAGGAT 
CCACCGATGG CCTTGGAGCC GCCTTACAGA CCGGACAGCT CGTTCAATCC ACTAACGCAT 
CCTTGCAAAT CCTTTCAGCA CATCCTCTGG CCAGTCATTC GAAAGTATGT CCTGTGGACT 
TTGCTCATAT TGATCGTTAT CCTGGGATTG GTGCTCTTCT TTTCCAATCT GCCCGCCAAA 
CTCATGACCA TTCCACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137919.1
CDS	130..5073
Translation	MSYMDDCFAGAPQEFLICITVHKAAQVGVTTGELYVRISLDKMTKSTKSFPNTENPFFNEYFVFEFHCTLTELLRLTILFELKKHMTYKKNVVVGELLVDLHSVWNQSNHGYFKKWGRLEAPIGENQTLENAVAPHGYLQLDLAIVSQHSPVSYALGSEEQDTQNLNKWTVDHDFDDIEKNLLTNVNIFAPSNIRYFVAFYRGYFIKQSNYMIQVSFAGFNGKTPVVKNTTTPVWNYEINFAWMYPSVAQRFLILIFAHEHLQWKCVAEFELCLEEIAFNGTPSLGPTYLHLYDPVNPMIYVGRLLMELRSEMLTDSQPTHTLSSKTVPGLDETRFWHDDVYLIEFLPLMGHHILTSGTAYKISARLAEHTSNVLEGQLRTPLDRFKSAMNSKNFRREAPFQSCMFRVRLPDNRAKYESDFFMLDIVNYMRSELDTFKLFQRKYPLQDDEQAKCLKVIVNNILTRIKEGLDAHRFDHDVQPQRTTWDINRQLYLLDYFAKLPLELQNLKQKLRSCFLEHLDTSIGDVVNELQRLVAEIGSLSSMARTQDEWPELVLSLSASGKDVGVCRLNAKLFLNLRNQDADQDQETLCWRLKSFSFKSAGCSHTCPNCGCTTAIVSGCLSIVVERERKRFLAVVNKEWANIEPMLWQLNPEQTHFLCHVYIHQAKVRPGGEKKNICDSHLRVLFADQACETYTSPGTLSPIWNAVITFNWVSLPGGIGLYLKNPPLLSLEFYNSERSLPEDLVGMGSVAMSVISEDRASDSNWEDAGVFGCSKNPLRKLQRLKYLTPPPLKWVPIARKGTLQAEVLMSAEIIQISMESLTTGQNDKKEPQLATGIPMAIRPTMQNFVLEVFFVGLRNYSKSSMSSAGKRKINLMMGDLVLSSGPSTARIRNSINFLVAYASGVVSLPDQQDYWPAIIATDVLLSGFSSESILGAALIPNSSNFLQRDRTLKCVFKKEQEAAEASTTVSVDEDDEDEEEELEWEEEETKPSRISSWWMRLLFAFGLRPAPYANRHALRFHDDLDDHELVEEREFTWWTKFYNSMYWSAAEMCHEHKHRLVIYYEELEKQAQFGYLQDWAVPVQLVHGVKFKKHGPPKEDIYATLKLQLKLTPCQCPVLEDPGGGGDMVRNMSNPIHPRHQSTLQSLGETVKLLVRVYVVQGVQMRPRDVKGDSDCYVKLFLGGKTFSDRAHFSPNHSNPVFGRLFEMEATLPGDHMLQVMVYDHDKIKDEVIGQTNIDLEDRWRTRHRATVGLANEYTKSGYNHWHDVKLPSEILIDLCQKRGIQAPYYYGNVIEVDGMLFGDETLISKDEELQERLSLAVLKNMDKLPSFGYKLVPEHVETRSLCRDDFPNVEQGKLQLWIELFEANIYVPSPIDITPVPPADFEVRVVVKNLVGIQAGDKNIFGKLMSDVYVIGWCEDEDKRQSTDIHYRSFAGDAAFNWRMVFGLKYSPNEDLMVIRRKAGLLEEIEFKKPPIIYLQVWDKDVLTKDEYLAALELNLSNMYAPYPAAQLCKPYPKKRKRINLFKRKVINGWFPLQSNAHPSQRSQAAVQNGKMQLSIEVCTDVEAVNRPAGRGQDPPMALEPPYRPDSSFNPLTHPCKSFQHILWPVIRKYVLWTLLILIVILGLVLFFSNLPAKLMTIPLE

Target ORF information:

RefSeq Version	NM_001144447.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster misfire (mfr), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144447.2

ORF Insert Sequence:

ATGAGTTACA TGGACGACTG TTTCGCAGGT GCTCCGCAGG AGTTTCTCAT CTGCATCACG 
GTGCACAAGG CTGCCCAAGT GGGAGTGACC ACCGGCGAGC TGTACGTGCG GATTAGCTTG 
GACAAAATGA CGAAGAGCAC CAAATCCTTT CCTAATACAG AAAATCCCTT CTTCAATGAG 
TACTTCGTTT TTGAGTTCCA TTGTACGTTA ACCGAACTCC TGCGCTTAAC GATCCTTTTT 
GAGCTAAAGA AACACATGAC GTATAAGAAA AATGTGGTAG TGGGTGAACT ACTGGTTGAT 
CTACATAGTG TGTGGAACCA GTCCAATCAT GGCTACTTCA AGAAGTGGGG TCGCCTGGAA 
GCTCCTATTG GAGAAAACCA GACCCTAGAG AATGCCGTTG CACCGCACGG CTATTTGCAA 
CTGGATCTGG CCATCGTATC CCAGCACAGT CCGGTGTCTT ATGCCCTCGG ATCTGAGGAG 
CAGGACACAC AAAATCTGAA CAAGTGGACG GTTGATCATG ATTTTGATGA CATCGAAAAG 
AATCTACTGA CCAACGTGAA CATCTTCGCC CCAAGTAATA TTCGATACTT CGTCGCCTTT 
TACCGGGGTT ACTTCATCAA GCAGAGCAAC TACATGATTC AAGTATCGTT TGCCGGCTTC 
AATGGCAAAA CGCCGGTGGT CAAGAACACG ACGACGCCCG TGTGGAACTA CGAGATCAAC 
TTTGCCTGGA TGTATCCGTC GGTGGCCCAG CGATTCCTCA TCCTAATTTT TGCCCATGAA 
CATCTGCAAT GGAAATGCGT GGCTGAGTTT GAATTGTGTC TTGAGGAGAT CGCGTTTAAT 
GGAACTCCTT CTCTGGGACC CACCTACTTG CATCTCTACG ATCCTGTCAA TCCTATGATC 
TATGTTGGTC GTCTTCTAAT GGAACTTCGG TCTGAAATGC TAACGGATTC CCAGCCCACA 
CACACGCTGT CCTCCAAGAC GGTGCCAGGT TTGGATGAGA CCCGTTTCTG GCATGACGAT 
GTCTATCTGA TTGAGTTTCT TCCACTAATG GGTCACCACA TCCTGACCTC GGGTACGGCA 
TACAAGATCT CGGCGAGATT AGCCGAACAC ACCTCGAACG TGCTGGAGGG TCAACTAAGG 
ACACCTTTAG ATCGATTCAA GTCGGCAATG AACTCAAAGA ATTTCCGGCG GGAGGCACCT 
TTTCAGTCCT GCATGTTTCG TGTCCGTTTG CCGGACAATC GAGCCAAGTA CGAAAGTGAT 
TTCTTCATGC TGGATATAGT CAATTATATG CGTTCAGAAC TGGACACCTT CAAGCTCTTT 
CAACGCAAAT ATCCACTCCA GGATGATGAG CAGGCCAAAT GCCTGAAGGT CATTGTGAAC 
AATATTTTGA CCAGAATCAA GGAGGGTCTA GATGCCCACC GTTTCGACCA CGACGTGCAA 
CCACAGCGCA CCACCTGGGA TATCAATCGG CAGCTGTACC TCCTGGACTA TTTCGCCAAG 
TTGCCACTGG AACTTCAAAA TCTTAAACAG AAACTACGCA GCTGCTTTCT GGAACACCTA 
GACACTTCCA TTGGCGATGT GGTTAATGAG CTACAGCGTT TGGTAGCTGA AATCGGATCC 
CTTTCCAGTA TGGCACGCAC CCAGGACGAG TGGCCAGAAT TGGTGCTTTC CTTAAGTGCA 
AGCGGTAAAG ATGTGGGTGT TTGCCGATTG AACGCCAAAC TCTTTTTGAA TCTACGAAAT 
CAGGATGCGG ATCAGGATCA GGAGACCCTT TGTTGGCGTC TAAAGAGCTT TTCCTTCAAG 
TCCGCGGGCT GTTCGCACAC CTGCCCCAAT TGTGGTTGCA CCACGGCCAT AGTATCAGGA 
TGTTTATCCA TTGTTGTGGA ACGGGAGCGA AAACGATTTC TTGCGGTTGT CAATAAAGAG 
TGGGCCAACA TTGAGCCCAT GTTGTGGCAG CTGAATCCTG AACAGACACA CTTCCTCTGT 
CACGTTTACA TACACCAAGC CAAAGTTCGT CCTGGTGGTG AAAAAAAGAA CATATGTGAC 
TCACATCTGA GAGTCCTGTT TGCCGACCAG GCATGTGAAA CGTATACGTC TCCCGGTACC 
CTATCGCCAA TTTGGAATGC TGTGATCACA TTTAACTGGG TGTCACTACC AGGAGGCATT 
GGTCTCTATC TAAAGAACCC TCCTCTACTT TCCTTGGAAT TCTATAATTC GGAACGCAGT 
CTACCAGAAG ACCTTGTGGG CATGGGAAGC GTGGCCATGA GTGTTATTAG CGAGGATCGG 
GCAAGTGATT CCAATTGGGA GGATGCAGGA GTATTCGGTT GCTCAAAGAA TCCGTTGCGA 
AAACTACAGA GATTGAAGTA CCTGACACCA CCTCCTCTCA AATGGGTGCC AATTGCTAGA 
AAAGGAACTC TACAAGCAGA GGTTTTGATG TCAGCCGAGA TCATTCAAAT CTCCATGGAA 
TCTCTGACCA CTGGTCAGAA TGATAAAAAG GAACCACAAC TTGCAACGGG CATTCCAATG 
GCCATCAGGC CAACTATGCA GAATTTTGTC TTAGAGGTTT TCTTCGTGGG TCTACGCAAT 
TACTCCAAGA GCAGCATGAG TTCTGCTGGT AAGCGGAAAA TCAATTTAAT GATGGGTGAT 
CTGGTCCTGT CCAGTGGACC CTCCACAGCA CGCATCCGGA ACAGCATAAA CTTTTTGGTG 
GCCTACGCAT CTGGAGTGGT TAGTTTGCCA GATCAACAGG ATTACTGGCC GGCGATCATA 
GCCACCGATG TCCTTCTCTC CGGATTTAGC AGCGAGTCCA TTCTGGGTGC AGCTCTCATA 
CCCAATTCAT CAAATTTCCT GCAACGCGAC AGGACCCTCA AGTGTGTGTT CAAAAAGGAA 
CAAGAGGCAG CAGAAGCTTC CACCACGGTT AGTGTGGATG AAGATGATGA GGATGAGGAA 
GAGGAACTGG AGTGGGAAGA GGAGGAGACC AAACCCTCCA GGATTAGCAG CTGGTGGATG 
CGACTGCTCT TCGCGTTCGG TCTGCGTCCA GCCCCCTATG CCAATCGACA CGCTTTAAGG 
TTCCACGATG ATTTGGATGA CCACGAATTG GTCGAGGAGC GAGAGTTCAC CTGGTGGACT 
AAGTTCTACA ACTCGATGTA TTGGAGTGCG GCCGAAATGT GCCACGAACA CAAGCACCGC 
CTGGTTATCT ACTACGAAGA GCTGGAGAAG CAGGCCCAGT TTGGATACCT CCAGGATTGG 
GCGGTTCCCG TTCAGTTGGT TCATGGCGTG AAGTTTAAAA AGCATGGTCC ACCCAAAGAA 
GATATCTATG CCACCTTAAA GCTGCAACTC AAGTTGACCC CTTGTCAGTG CCCGGTTCTT 
GAGGATCCCG GAGGTGGTGG CGACATGGTT CGCAACATGT CAAATCCCAT ACATCCTCGA 
CATCAGTCCA CACTACAGTC GCTGGGTGAG ACCGTAAAAC TATTGGTACG TGTTTATGTA 
GTCCAGGGGG TTCAGATGCG TCCTCGAGAT GTGAAAGGTG ACTCCGATTG CTATGTGAAG 
CTGTTCCTGG GCGGCAAGAC GTTCTCCGAT AGAGCCCATT TCTCACCCAA CCACAGTAAT 
CCAGTTTTCG GTCGTCTATT TGAAATGGAG GCTACGCTGC CAGGAGATCA TATGCTGCAA 
GTCATGGTAT ATGATCATGA CAAGATCAAG GACGAGGTGA TCGGTCAGAC AAACATCGAT 
CTGGAGGATC GCTGGCGAAC TAGGCATCGT GCCACAGTGG GTTTGGCCAA TGAGTACACA 
AAGAGTGGCT ATAATCATTG GCACGACGTT AAGTTGCCCT CGGAAATTCT CATAGATCTG 
TGCCAGAAAA GAGGCATTCA GGCCCCTTAC TATTATGGTA ATGTAATTGA GGTGGACGGA 
ATGCTTTTTG GGGACGAAAC TTTGATCTCA AAAGATGAGG AGCTGCAGGA GAGACTTAGT 
TTAGCCGTGC TTAAGAATAT GGATAAGTTG CCATCCTTTG GTTACAAATT GGTTCCCGAA 
CATGTGGAAA CCCGCTCCCT TTGCCGCGAT GATTTTCCAA ATGTCGAACA GGGCAAACTA 
CAACTGTGGA TAGAGCTGTT CGAGGCGAAC ATCTATGTGC CCAGTCCCAT CGATATAACC 
CCTGTGCCGC CCGCGGATTT CGAGGTGCGT GTTGTGGTTA AGAATCTGGT CGGGATTCAG 
GCGGGCGACA AAAACATTTT TGGCAAACTA ATGAGCGACG TTTACGTGAT AGGATGGTGC 
GAGGATGAGG ACAAGCGCCA ATCGACCGAT ATCCATTACC GCTCATTTGC AGGAGATGCG 
GCATTTAATT GGCGCATGGT CTTCGGCCTC AAGTATTCCC CCAACGAGGA CCTGATGGTA 
ATCCGGCGAA AGGCGGGGCT GCTGGAGGAA ATCGAGTTCA AGAAGCCGCC TATTATCTAT 
CTGCAGGTGT GGGACAAGGA TGTGTTAACC AAGGATGAGT ATCTGGCCGC ACTGGAGCTC 
AATCTCTCCA ACATGTACGC ACCGTATCCA GCTGCCCAGT TATGTAAGCC ATATCCCAAG 
AAGAGGAAGC GCATCAATCT CTTCAAGCGC AAGGTCATCA ACGGCTGGTT TCCGCTGCAA 
AGTAATGCAC ATCCATCGCA ACGCAGCCAG GCCGCAGTTC AAAATGGCAA AATGCAGCTG 
AGCATCGAAG TCTGCACGGA TGTGGAGGCC GTCAACCGGC CGGCTGGTCG TGGACAGGAT 
CCACCGATGG CCTTGGAGCC GCCTTACAGA CCGGACAGCT CGTTCAATCC ACTAACGCAT 
CCTTGCAAAT CCTTTCAGCA CATCCTCTGG CCAGTCATTC GAAAGTATGT CCTGTGGACT 
TTGCTCATAT TGATCGTTAT CCTGGGATTG GTGCTCTTCT TTTCCAATCT GCCCGCCAAA 
CTCATGACCA TTCCACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mfr ( NM_001144445.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137917.1 Drosophila melanogaster misfire (mfr), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa17954
Clone ID Related Accession (Same CDS sequence)	NM_001144445.2
Accession Version	NM_001144445.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	misfire, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001144445.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACATCT GCAATGGAAA TGCGTGGCTG AGTTTGAATT GTGTCTTGAG GAGATCGCGT 
TTAATGCCCA CACACACGCT GTCCTCCAAG ACGGTGCCAG GTTTGGATGA GACCCGTTTC 
TGGCATGACG ATGTCTATCT GATTGAGTTT CTTCCACTAA TGGGTCACCA CATCCTGACC 
TCGGGTACGG CATACAAGAT CTCGGCGAGA TTAGCCGAAC ACACCTCGAA CGTGCTGGAG 
GGTCAACTAA GGACACCTTT AGATCGATTC AAGTCGGCAA TGAACTCAAA GAATTTCCGG 
CGGGAGGCAC CTTTTCAGTC CTGCATGTTT CGTGTCCGTT TGCCGGACAA TCGAGCCAAG 
TACGAAAGTG ATTTCTTCAT GCTGGATATA GTCAATTATA TGCGTTCAGA ACTGGACACC 
TTCAAGCTCT TTCAACGCAA ATATCCACTC CAGGATGATG AGCAGGCCAA ATGCCTGAAG 
GTCATTGTGA ACAATATTTT GACCAGAATC AAGGAGGGTC TAGATGCCCA CCGTTTCGAC 
CACGACGTGC AACCACAGCG CACCACCTGG GATATCAATC GGCAGCTGTA CCTCCTGGAC 
TATTTCGCCA AGTTGCCACT GGAACTTCAA AATCTTAAAC AGAAACTACG CAGCTGCTTT 
CTGGAACACC TAGACACTTC CATTGGCGAT GTGGTTAATG AGCTACAGCG TTTGGTAGCT 
GAAATCGGAT CCCTTTCCAG TATGGCACGC ACCCAGGACG AGTGGCCAGA ATTGGTGCTT 
TCCTTAAGTG CAAGCGGTAA AGATGTGGGT GTTTGCCGAT TGAACGCCAA ACTCTTTTTG 
AATCTACGAA ATCAGGATGC GGATCAGGAT CAGGAGACCC TTTGTTGGCG TCTAAAGAGC 
TTTTCCTTCA AGTCCGCGGG CTGTTCGCAC ACCTGCCCCA ATTGTGGTTG CACCACGGCC 
ATAGTATCAG GATGTTTATC CATTGTTGTG GAACGGGAGC GAAAACGATT TCTTGCGGTT 
GTCAATAAAG AGTGGGCCAA CATTGAGCCC ATGTTGTGGC AGCTGAATCC TGAACAGACA 
CACTTCCTCT GTCACGTTTA CATACACCAA GCCAAAGTTC GTCCTGGTGG TGAAAAAAAG 
AACATATGTG ACTCACATCT GAGAGTCCTG TTTGCCGACC AGGCATGTGA AACGTATACG 
TCTCCCGGTA CCCTATCGCC AATTTGGAAT GCTGTGATCA CATTTAACTG GGTGTCACTA 
CCAGGAGGCA TTGGTCTCTA TCTAAAGAAC CCTCCTCTAC TTTCCTTGGA ATTCTATAAT 
TCGGAACGCA GTCTACCAGA AGACCTTGTG GGCATGGGAA GCGTGGCCAT GAGTGTTATT 
AGCGAGGATC GGGCAAGTGA TTCCAATTGG GAGGATGCAG GAGTATTCGG TTGCTCAAAG 
AATCCGTTGC GAAAACTACA GAGATTGAAG TACCTGACAC CACCTCCTCT CAAATGGGTG 
CCAATTGCTA GAAAAGGAAC TCTACAAGCA GAGGTTTTGA TGTCAGCCGA GATCATTCAA 
ATCTCCATGG AATCTCTGAC CACTGGTCAG AATGATAAAA AGGAACCACA ACTTGCAACG 
GGCATTCCAA TGGCCATCAG GCCAACTATG CAGAATTTTG TCTTAGAGGT TTTCTTCGTG 
GGTCTACGCA ATTACTCCAA GAGCAGCATG AGTTCTGCTG GTAAGCGGAA AATCAATTTA 
ATGATGGGTG ATCTGGTCCT GTCCAGTGGA CCCTCCACAG CACGCATCCG GAACAGCATA 
AACTTTTTGG TGGCCTACGC ATCTGGAGTG GTTAGTTTGC CAGATCAACA GGATTACTGG 
CCGGCGATCA TAGCCACCGA TGTCCTTCTC TCCGGATTTA GCAGCGAGTC CATTCTGGGT 
GCAGCTCTCA TACCCAATTC ATCAAATTTC CTGCAACGCG ACAGGACCCT CAAGTGTGTG 
TTCAAAAAGG AACAAGAGGC AGCAGAAGCT TCCACCACGG TTAGTGTGGA TGAAGATGAT 
GAGGATGAGG AAGAGGAACT GGAGTGGGAA GAGGAGGAGA CCAAACCCTC CAGGATTAGC 
AGCTGGTGGA TGCGACTGCT CTTCGCGTTC GGTCTGCGTC CAGCCCCCTA TGCCAATCGA 
CACGCTTTAA GGTTCCACGA TGATTTGGAT GACCACGAAT TGGTCGAGGA GCGAGAGTTC 
ACCTGGTGGA CTAAGTTCTA CAACTCGATG TATTGGAGTG CGGCCGAAAT GTGCCACGAA 
CACAAGCACC GCCTGGTTAT CTACTACGAA GAGCTGGAGA AGCAGGCCCA GTTTGGATAC 
CTCCAGGATT GGGCGGTTCC CGTTCAGTTG GTTCATGGCG TGAAGTTTAA AAAGCATGGT 
CCACCCAAAG AAGATATCTA TGCCACCTTA AAGCTGCAAC TCAAGTTGAC CCCTTGTCAG 
TGCCCGGTTC TTGAGGATCC CGGAGGTGGT GGCGACATGG TTCGCAACAT GTCAAATCCC 
ATACATCCTC GACATCAGTC CACACTACAG TCGCTGGGTG AGACCGTAAA ACTATTGGTA 
CGTGTTTATG TAGTCCAGGG GGTTCAGATG CGTCCTCGAG ATGTGAAAGG TGACTCCGAT 
TGCTATGTGA AGCTGTTCCT GGGCGGCAAG ACGTTCTCCG ATAGAGCCCA TTTCTCACCC 
AACCACAGTA ATCCAGTTTT CGGTCGTCTA TTTGAAATGG AGGCTACGCT GCCAGGAGAT 
CATATGCTGC AAGTCATGGT ATATGATCAT GACAAGATCA AGGACGAGGT GATCGGTCAG 
ACAAACATCG ATCTGGAGGA TCGCTGGCGA ACTAGGCATC GTGCCACAGT GGGTTTGGCC 
AATGAGTACA CAAAGAGTGG CTATAATCAT TGGCACGACG TTAAGTTGCC CTCGGAAATT 
CTCATAGATC TGTGCCAGAA AAGAGGCATT CAGGCCCCTT ACTATTATGG TAATGTAATT 
GAGGTGGACG GAATGCTTTT TGGGGACGAA ACTTTGATCT CAAAAGATGA GGAGCTGCAG 
GAGAGACTTA GTTTAGCCGT GCTTAAGAAT ATGGATAAGT TGCCATCCTT TGGTTACAAA 
TTGGTTCCCG AACATGTGGA AACCCGCTCC CTTTGCCGCG ATGATTTTCC AAATGTCGAA 
CAGGGCAAAC TACAACTGTG GATAGAGCTG TTCGAGGCGA ACATCTATGT GCCCAGTCCC 
ATCGATATAA CCCCTGTGCC GCCCGCGGAT TTCGAGGTGC GTGTTGTGGT TAAGAATCTG 
GTCGGGATTC AGGCGGGCGA CAAAAACATT TTTGGCAAAC TAATGAGCGA CGTTTACGTG 
ATAGGATGGT GCGAGGATGA GGACAAGCGC CAATCGACCG ATATCCATTA CCGCTCATTT 
GCAGGAGATG CGGCATTTAA TTGGCGCATG GTCTTCGGCC TCAAGTATTC CCCCAACGAG 
GACCTGATGG TAATCCGGCG AAAGGCGGGG CTGCTGGAGG AAATCGAGTT CAAGAAGCCG 
CCTATTATCT ATCTGCAGGT GTGGGACAAG GATGTGTTAA CCAAGGATGA GTATCTGGCC 
GCACTGGAGC TCAATCTCTC CAACATGTAC GCACCGTATC CAGCTGCCCA GTTATGTAAG 
CCATATCCCA AGAAGAGGAA GCGCATCAAT CTCTTCAAGC GCAAGGTCAT CAACGGCTGG 
TTTCCGCTGC AAAGTAATGC ACATCCATCG CAACGCAGCC AGGCCGCAGT TCAAAATGGC 
AAAATGCAGC TGAGCATCGA AGTCTGCACG GATGTGGAGG CCGTCAACCG GCCGGCTGGT 
CGTGGACAGG ATCCACCGAT GGCCTTGGAG CCGCCTTACA GACCGGACAG CTCGTTCAAT 
CCACTAACGC ATCCTTGCAA ATCCTTTCAG CACATCCTCT GGCCAGTCAT TCGAAAGTAT 
GTCCTGTGGA CTTTGCTCAT ATTGATCGTT ATCCTGGGAT TGGTGCTCTT CTTTTCCAAT 
CTGCCCGCCA AACTCATGAC CATTCCACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137917.1
CDS	951..5006
Translation	MNICNGNAWLSLNCVLRRSRLMPTHTLSSKTVPGLDETRFWHDDVYLIEFLPLMGHHILTSGTAYKISARLAEHTSNVLEGQLRTPLDRFKSAMNSKNFRREAPFQSCMFRVRLPDNRAKYESDFFMLDIVNYMRSELDTFKLFQRKYPLQDDEQAKCLKVIVNNILTRIKEGLDAHRFDHDVQPQRTTWDINRQLYLLDYFAKLPLELQNLKQKLRSCFLEHLDTSIGDVVNELQRLVAEIGSLSSMARTQDEWPELVLSLSASGKDVGVCRLNAKLFLNLRNQDADQDQETLCWRLKSFSFKSAGCSHTCPNCGCTTAIVSGCLSIVVERERKRFLAVVNKEWANIEPMLWQLNPEQTHFLCHVYIHQAKVRPGGEKKNICDSHLRVLFADQACETYTSPGTLSPIWNAVITFNWVSLPGGIGLYLKNPPLLSLEFYNSERSLPEDLVGMGSVAMSVISEDRASDSNWEDAGVFGCSKNPLRKLQRLKYLTPPPLKWVPIARKGTLQAEVLMSAEIIQISMESLTTGQNDKKEPQLATGIPMAIRPTMQNFVLEVFFVGLRNYSKSSMSSAGKRKINLMMGDLVLSSGPSTARIRNSINFLVAYASGVVSLPDQQDYWPAIIATDVLLSGFSSESILGAALIPNSSNFLQRDRTLKCVFKKEQEAAEASTTVSVDEDDEDEEEELEWEEEETKPSRISSWWMRLLFAFGLRPAPYANRHALRFHDDLDDHELVEEREFTWWTKFYNSMYWSAAEMCHEHKHRLVIYYEELEKQAQFGYLQDWAVPVQLVHGVKFKKHGPPKEDIYATLKLQLKLTPCQCPVLEDPGGGGDMVRNMSNPIHPRHQSTLQSLGETVKLLVRVYVVQGVQMRPRDVKGDSDCYVKLFLGGKTFSDRAHFSPNHSNPVFGRLFEMEATLPGDHMLQVMVYDHDKIKDEVIGQTNIDLEDRWRTRHRATVGLANEYTKSGYNHWHDVKLPSEILIDLCQKRGIQAPYYYGNVIEVDGMLFGDETLISKDEELQERLSLAVLKNMDKLPSFGYKLVPEHVETRSLCRDDFPNVEQGKLQLWIELFEANIYVPSPIDITPVPPADFEVRVVVKNLVGIQAGDKNIFGKLMSDVYVIGWCEDEDKRQSTDIHYRSFAGDAAFNWRMVFGLKYSPNEDLMVIRRKAGLLEEIEFKKPPIIYLQVWDKDVLTKDEYLAALELNLSNMYAPYPAAQLCKPYPKKRKRINLFKRKVINGWFPLQSNAHPSQRSQAAVQNGKMQLSIEVCTDVEAVNRPAGRGQDPPMALEPPYRPDSSFNPLTHPCKSFQHILWPVIRKYVLWTLLILIVILGLVLFFSNLPAKLMTIPLE

Target ORF information:

RefSeq Version	NM_001144445.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster misfire (mfr), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144445.2

ORF Insert Sequence:

ATGAACATCT GCAATGGAAA TGCGTGGCTG AGTTTGAATT GTGTCTTGAG GAGATCGCGT 
TTAATGCCCA CACACACGCT GTCCTCCAAG ACGGTGCCAG GTTTGGATGA GACCCGTTTC 
TGGCATGACG ATGTCTATCT GATTGAGTTT CTTCCACTAA TGGGTCACCA CATCCTGACC 
TCGGGTACGG CATACAAGAT CTCGGCGAGA TTAGCCGAAC ACACCTCGAA CGTGCTGGAG 
GGTCAACTAA GGACACCTTT AGATCGATTC AAGTCGGCAA TGAACTCAAA GAATTTCCGG 
CGGGAGGCAC CTTTTCAGTC CTGCATGTTT CGTGTCCGTT TGCCGGACAA TCGAGCCAAG 
TACGAAAGTG ATTTCTTCAT GCTGGATATA GTCAATTATA TGCGTTCAGA ACTGGACACC 
TTCAAGCTCT TTCAACGCAA ATATCCACTC CAGGATGATG AGCAGGCCAA ATGCCTGAAG 
GTCATTGTGA ACAATATTTT GACCAGAATC AAGGAGGGTC TAGATGCCCA CCGTTTCGAC 
CACGACGTGC AACCACAGCG CACCACCTGG GATATCAATC GGCAGCTGTA CCTCCTGGAC 
TATTTCGCCA AGTTGCCACT GGAACTTCAA AATCTTAAAC AGAAACTACG CAGCTGCTTT 
CTGGAACACC TAGACACTTC CATTGGCGAT GTGGTTAATG AGCTACAGCG TTTGGTAGCT 
GAAATCGGAT CCCTTTCCAG TATGGCACGC ACCCAGGACG AGTGGCCAGA ATTGGTGCTT 
TCCTTAAGTG CAAGCGGTAA AGATGTGGGT GTTTGCCGAT TGAACGCCAA ACTCTTTTTG 
AATCTACGAA ATCAGGATGC GGATCAGGAT CAGGAGACCC TTTGTTGGCG TCTAAAGAGC 
TTTTCCTTCA AGTCCGCGGG CTGTTCGCAC ACCTGCCCCA ATTGTGGTTG CACCACGGCC 
ATAGTATCAG GATGTTTATC CATTGTTGTG GAACGGGAGC GAAAACGATT TCTTGCGGTT 
GTCAATAAAG AGTGGGCCAA CATTGAGCCC ATGTTGTGGC AGCTGAATCC TGAACAGACA 
CACTTCCTCT GTCACGTTTA CATACACCAA GCCAAAGTTC GTCCTGGTGG TGAAAAAAAG 
AACATATGTG ACTCACATCT GAGAGTCCTG TTTGCCGACC AGGCATGTGA AACGTATACG 
TCTCCCGGTA CCCTATCGCC AATTTGGAAT GCTGTGATCA CATTTAACTG GGTGTCACTA 
CCAGGAGGCA TTGGTCTCTA TCTAAAGAAC CCTCCTCTAC TTTCCTTGGA ATTCTATAAT 
TCGGAACGCA GTCTACCAGA AGACCTTGTG GGCATGGGAA GCGTGGCCAT GAGTGTTATT 
AGCGAGGATC GGGCAAGTGA TTCCAATTGG GAGGATGCAG GAGTATTCGG TTGCTCAAAG 
AATCCGTTGC GAAAACTACA GAGATTGAAG TACCTGACAC CACCTCCTCT CAAATGGGTG 
CCAATTGCTA GAAAAGGAAC TCTACAAGCA GAGGTTTTGA TGTCAGCCGA GATCATTCAA 
ATCTCCATGG AATCTCTGAC CACTGGTCAG AATGATAAAA AGGAACCACA ACTTGCAACG 
GGCATTCCAA TGGCCATCAG GCCAACTATG CAGAATTTTG TCTTAGAGGT TTTCTTCGTG 
GGTCTACGCA ATTACTCCAA GAGCAGCATG AGTTCTGCTG GTAAGCGGAA AATCAATTTA 
ATGATGGGTG ATCTGGTCCT GTCCAGTGGA CCCTCCACAG CACGCATCCG GAACAGCATA 
AACTTTTTGG TGGCCTACGC ATCTGGAGTG GTTAGTTTGC CAGATCAACA GGATTACTGG 
CCGGCGATCA TAGCCACCGA TGTCCTTCTC TCCGGATTTA GCAGCGAGTC CATTCTGGGT 
GCAGCTCTCA TACCCAATTC ATCAAATTTC CTGCAACGCG ACAGGACCCT CAAGTGTGTG 
TTCAAAAAGG AACAAGAGGC AGCAGAAGCT TCCACCACGG TTAGTGTGGA TGAAGATGAT 
GAGGATGAGG AAGAGGAACT GGAGTGGGAA GAGGAGGAGA CCAAACCCTC CAGGATTAGC 
AGCTGGTGGA TGCGACTGCT CTTCGCGTTC GGTCTGCGTC CAGCCCCCTA TGCCAATCGA 
CACGCTTTAA GGTTCCACGA TGATTTGGAT GACCACGAAT TGGTCGAGGA GCGAGAGTTC 
ACCTGGTGGA CTAAGTTCTA CAACTCGATG TATTGGAGTG CGGCCGAAAT GTGCCACGAA 
CACAAGCACC GCCTGGTTAT CTACTACGAA GAGCTGGAGA AGCAGGCCCA GTTTGGATAC 
CTCCAGGATT GGGCGGTTCC CGTTCAGTTG GTTCATGGCG TGAAGTTTAA AAAGCATGGT 
CCACCCAAAG AAGATATCTA TGCCACCTTA AAGCTGCAAC TCAAGTTGAC CCCTTGTCAG 
TGCCCGGTTC TTGAGGATCC CGGAGGTGGT GGCGACATGG TTCGCAACAT GTCAAATCCC 
ATACATCCTC GACATCAGTC CACACTACAG TCGCTGGGTG AGACCGTAAA ACTATTGGTA 
CGTGTTTATG TAGTCCAGGG GGTTCAGATG CGTCCTCGAG ATGTGAAAGG TGACTCCGAT 
TGCTATGTGA AGCTGTTCCT GGGCGGCAAG ACGTTCTCCG ATAGAGCCCA TTTCTCACCC 
AACCACAGTA ATCCAGTTTT CGGTCGTCTA TTTGAAATGG AGGCTACGCT GCCAGGAGAT 
CATATGCTGC AAGTCATGGT ATATGATCAT GACAAGATCA AGGACGAGGT GATCGGTCAG 
ACAAACATCG ATCTGGAGGA TCGCTGGCGA ACTAGGCATC GTGCCACAGT GGGTTTGGCC 
AATGAGTACA CAAAGAGTGG CTATAATCAT TGGCACGACG TTAAGTTGCC CTCGGAAATT 
CTCATAGATC TGTGCCAGAA AAGAGGCATT CAGGCCCCTT ACTATTATGG TAATGTAATT 
GAGGTGGACG GAATGCTTTT TGGGGACGAA ACTTTGATCT CAAAAGATGA GGAGCTGCAG 
GAGAGACTTA GTTTAGCCGT GCTTAAGAAT ATGGATAAGT TGCCATCCTT TGGTTACAAA 
TTGGTTCCCG AACATGTGGA AACCCGCTCC CTTTGCCGCG ATGATTTTCC AAATGTCGAA 
CAGGGCAAAC TACAACTGTG GATAGAGCTG TTCGAGGCGA ACATCTATGT GCCCAGTCCC 
ATCGATATAA CCCCTGTGCC GCCCGCGGAT TTCGAGGTGC GTGTTGTGGT TAAGAATCTG 
GTCGGGATTC AGGCGGGCGA CAAAAACATT TTTGGCAAAC TAATGAGCGA CGTTTACGTG 
ATAGGATGGT GCGAGGATGA GGACAAGCGC CAATCGACCG ATATCCATTA CCGCTCATTT 
GCAGGAGATG CGGCATTTAA TTGGCGCATG GTCTTCGGCC TCAAGTATTC CCCCAACGAG 
GACCTGATGG TAATCCGGCG AAAGGCGGGG CTGCTGGAGG AAATCGAGTT CAAGAAGCCG 
CCTATTATCT ATCTGCAGGT GTGGGACAAG GATGTGTTAA CCAAGGATGA GTATCTGGCC 
GCACTGGAGC TCAATCTCTC CAACATGTAC GCACCGTATC CAGCTGCCCA GTTATGTAAG 
CCATATCCCA AGAAGAGGAA GCGCATCAAT CTCTTCAAGC GCAAGGTCAT CAACGGCTGG 
TTTCCGCTGC AAAGTAATGC ACATCCATCG CAACGCAGCC AGGCCGCAGT TCAAAATGGC 
AAAATGCAGC TGAGCATCGA AGTCTGCACG GATGTGGAGG CCGTCAACCG GCCGGCTGGT 
CGTGGACAGG ATCCACCGAT GGCCTTGGAG CCGCCTTACA GACCGGACAG CTCGTTCAAT 
CCACTAACGC ATCCTTGCAA ATCCTTTCAG CACATCCTCT GGCCAGTCAT TCGAAAGTAT 
GTCCTGTGGA CTTTGCTCAT ATTGATCGTT ATCCTGGGAT TGGTGCTCTT CTTTTCCAAT 
CTGCCCGCCA AACTCATGAC CATTCCACTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mfr ( NM_001144448.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137920.1 Drosophila melanogaster misfire (mfr), transcript variant I, mRNA.

Price & Availability↑

CloneID	OFa17955
Clone ID Related Accession (Same CDS sequence)	NM_001144448.2
Accession Version	NM_001144448.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	misfire, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001144448.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTCCGT TTCTAAAGTA CAGATTCCAT CCTTTCAAGG GCAAAACGCC GGTGGTCAAG 
AACACGACGA CGCCCGTGTG GAACTACGAG ATCAACTTTG CCTGGATGTA TCCGTCGGTG 
GCCCAGCGAT TCCTCATCCT AATTTTTGCC CATGAACATC TGCAATGGAA ATGCGTGGCT 
GAGTTTGAAT TGTGTCTTGA GGAGATCGCG TTTAATGGAA CTCCTTCTCT GGGACCCACC 
TACTTGCATC TCTACGATCC TGTCAATCCT ATGATCTATG TTGGTCGTCT TCTAATGGAA 
CTTCGGTCTG AAATGCTAAC GGATTCCCAG CCCACACACA CGCTGTCCTC CAAGACGGTG 
CCAGGTTTGG ATGAGACCCG TTTCTGGCAT GACGATGTCT ATCTGATTGA GTTTCTTCCA 
CTAATGGGTC ACCACATCCT GACCTCGGGT ACGGCATACA AGATCTCGGC GAGATTAGCC 
GAACACACCT CGAACGTGCT GGAGGGTCAA CTAAGGACAC CTTTAGATCG ATTCAAGTCG 
GCAATGAACT CAAAGAATTT CCGGCGGGAG GCACCTTTTC AGTCCTGCAT GTTTCGTGTC 
CGTTTGCCGG ACAATCGAGC CAAGTACGAA AGTGATTTCT TCATGCTGGA TATAGTCAAT 
TATATGCGTT CAGAACTGGA CACCTTCAAG CTCTTTCAAC GCAAATATCC ACTCCAGGAT 
GATGAGCAGG CCAAATGCCT GAAGGTCATT GTGAACAATA TTTTGACCAG AATCAAGGAG 
GGTCTAGATG CCCACCGTTT CGACCACGAC GTGCAACCAC AGCGCACCAC CTGGGATATC 
AATCGGCAGC TGTACCTCCT GGACTATTTC GCCAAGTTGC CACTGGAACT TCAAAATCTT 
AAACAGAAAC TACGCAGCTG CTTTCTGGAA CACCTAGACA CTTCCATTGG CGATGTGGTT 
AATGAGCTAC AGCGTTTGGT AGCTGAAATC GGATCCCTTT CCAGTATGGC ACGCACCCAG 
GACGAGTGGC CAGAATTGGT GCTTTCCTTA AGTGCAAGCG GTAAAGATGT GGGTGTTTGC 
CGATTGAACG CCAAACTCTT TTTGAATCTA CGAAATCAGG ATGCGGATCA GGATCAGGAG 
ACCCTTTGTT GGCGTCTAAA GAGCTTTTCC TTCAAGTCCG CGGGCTGTTC GCACACCTGC 
CCCAATTGTG GTTGCACCAC GGCCATAGTA TCAGGATGTT TATCCATTGT TGTGGAACGG 
GAGCGAAAAC GATTTCTTGC GGTTGTCAAT AAAGAGTGGG CCAACATTGA GCCCATGTTG 
TGGCAGCTGA ATCCTGAACA GACACACTTC CTCTGTCACG TTTACATACA CCAAGCCAAA 
GTTCGTCCTG GTGGTGAAAA AAAGAACATA TGTGACTCAC ATCTGAGAGT CCTGTTTGCC 
GACCAGGCAT GTGAAACGTA TACGTCTCCC GGTACCCTAT CGCCAATTTG GAATGCTGTG 
ATCACATTTA ACTGGGTGTC ACTACCAGGA GGCATTGGTC TCTATCTAAA GAACCCTCCT 
CTACTTTCCT TGGAATTCTA TAATTCGGAA CGCAGTCTAC CAGAAGACCT TGTGGGCATG 
GGAAGCGTGG CCATGAGTGT TATTAGCGAG GATCGGGCAA GTGATTCCAA TTGGGAGGAT 
GCAGGAGTAT TCGGTTGCTC AAAGAATCCG TTGCGAAAAC TACAGAGATT GAAGTACCTG 
ACACCACCTC CTCTCAAATG GGTGCCAATT GCTAGAAAAG GAACTCTACA AGCAGAGGTT 
TTGATGTCAG CCGAGATCAT TCAAATCTCC ATGGAATCTC TGACCACTGG TCAGAATGAT 
AAAAAGGAAC CACAACTTGC AACGGGCATT CCAATGGCCA TCAGGCCAAC TATGCAGAAT 
TTTGTCTTAG AGGTTTTCTT CGTGGGTCTA CGCAATTACT CCAAGAGCAG CATGAGTTCT 
GCTGGTAAGC GGAAAATCAA TTTAATGATG GGTGATCTGG TCCTGTCCAG TGGACCCTCC 
ACAGCACGCA TCCGGAACAG CATAAACTTT TTGGTGGCCT ACGCATCTGG AGTGGTTAGT 
TTGCCAGATC AACAGGATTA CTGGCCGGCG ATCATAGCCA CCGATGTCCT TCTCTCCGGA 
TTTAGCAGCG AGTCCATTCT GGGTGCAGCT CTCATACCCA ATTCATCAAA TTTCCTGCAA 
CGCGACAGGA CCCTCAAGTG TGTGTTCAAA AAGGAACAAG AGGCAGCAGA AGCTTCCACC 
ACGGTTAGTG TGGATGAAGA TGATGAGGAT GAGGAAGAGG AACTGGAGTG GGAAGAGGAG 
GAGACCAAAC CCTCCAGGAT TAGCAGCTGG TGGATGCGAC TGCTCTTCGC GTTCGGTCTG 
CGTCCAGCCC CCTATGCCAA TCGACACGCT TTAAGGTTCC ACGATGATTT GGATGACCAC 
GAATTGGTCG AGGAGCGAGA GTTCACCTGG TGGACTAAGT TCTACAACTC GATGTATTGG 
AGTGCGGCCG AAATGTGCCA CGAACACAAG CACCGCCTGG TTATCTACTA CGAAGAGCTG 
GAGAAGCAGG CCCAGTTTGG ATACCTCCAG GATTGGGCGG TTCCCGTTCA GTTGGTTCAT 
GGCGTGAAGT TTAAAAAGCA TGGTCCACCC AAAGAAGATA TCTATGCCAC CTTAAAGCTG 
CAACTCAAGT TGACCCCTTG TCAGTGCCCG GTTCTTGAGG ATCCCGGAGG TGGTGGCGAC 
ATGGTTCGCA ACATGTCAAA TCCCATACAT CCTCGACATC AGTCCACACT ACAGTCGCTG 
GGTGAGACCG TAAAACTATT GGTACGTGTT TATGTAGTCC AGGGGGTTCA GATGCGTCCT 
CGAGATGTGA AAGGTGACTC CGATTGCTAT GTGAAGCTGT TCCTGGGCGG CAAGACGTTC 
TCCGATAGAG CCCATTTCTC ACCCAACCAC AGTAATCCAG TTTTCGGTCG TCTATTTGAA 
ATGGAGGCTA CGCTGCCAGG AGATCATATG CTGCAAGTCA TGGTATATGA TCATGACAAG 
ATCAAGGACG AGGTGATCGG TCAGACAAAC ATCGATCTGG AGGATCGCTG GCGAACTAGG 
CATCGTGCCA CAGTGGGTTT GGCCAATGAG TACACAAAGA GTGGCTATAA TCATTGGCAC 
GACGTTAAGT TGCCCTCGGA AATTCTCATA GATCTGTGCC AGAAAAGAGG CATTCAGGCC 
CCTTACTATT ATGGTAATGT AATTGAGGTG GACGGAATGC TTTTTGGGGA CGAAACTTTG 
ATCTCAAAAG ATGAGGAGCT GCAGGAGAGA CTTAGTTTAG CCGTGCTTAA GAATATGGAT 
AAGTTGCCAT CCTTTGGTTA CAAATTGGTT CCCGAACATG TGGAAACCCG CTCCCTTTGC 
CGCGATGATT TTCCAAATGT CGAACAGGGC AAACTACAAC TGTGGATAGA GCTGTTCGAG 
GCGAACATCT ATGTGCCCAG TCCCATCGAT ATAACCCCTG TGCCGCCCGC GGATTTCGAG 
GTGCGTGTTG TGGTTAAGAA TCTGGTCGGG ATTCAGGCGG GCGACAAAAA CATTTTTGGC 
AAACTAATGA GCGACGTTTA CGTGATAGGA TGGTGCGAGG ATGAGGACAA GCGCCAATCG 
ACCGATATCC ATTACCGCTC ATTTGCAGGA GATGCGGCAT TTAATTGGCG CATGGTCTTC 
GGCCTCAAGT ATTCCCCCAA CGAGGACCTG ATGGTAATCC GGCGAAAGGC GGGGCTGCTG 
GAGGAAATCG AGTTCAAGAA GCCGCCTATT ATCTATCTGC AGGTGTGGGA CAAGGATGTG 
TTAACCAAGG ATGAGTATCT GGCCGCACTG GAGCTCAATC TCTCCAACAT GTACGCACCG 
TATCCAGCTG CCCAGTTATG TAAGCCATAT CCCAAGAAGA GGAAGCGCAT CAATCTCTTC 
AAGCGCAAGG TCATCAACGG CTGGTTTCCG CTGCAAAGTA ATGCACATCC ATCGCAACGC 
AGCCAGGCCG CAGTTCAAAA TGGCAAAATG CAGCTGAGCA TCGAAGTCTG CACGGATGTG 
GAGGCCGTCA ACCGGCCGGC TGGTCGTGGA CAGGATCCAC CGATGGCCTT GGAGCCGCCT 
TACAGACCGG ACAGCTCGTT CAATCCACTA ACGCATCCTT GCAAATCCTT TCAGCACATC 
CTCTGGCCAG TCATTCGAAA GTATGTCCTG TGGACTTTGC TCATATTGAT CGTTATCCTG 
GGATTGGTGC TCTTCTTTTC CAATCTGCCC GCCAAACTCA TGACCATTCC ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137920.1
CDS	800..5119
Translation	MFPFLKYRFHPFKGKTPVVKNTTTPVWNYEINFAWMYPSVAQRFLILIFAHEHLQWKCVAEFELCLEEIAFNGTPSLGPTYLHLYDPVNPMIYVGRLLMELRSEMLTDSQPTHTLSSKTVPGLDETRFWHDDVYLIEFLPLMGHHILTSGTAYKISARLAEHTSNVLEGQLRTPLDRFKSAMNSKNFRREAPFQSCMFRVRLPDNRAKYESDFFMLDIVNYMRSELDTFKLFQRKYPLQDDEQAKCLKVIVNNILTRIKEGLDAHRFDHDVQPQRTTWDINRQLYLLDYFAKLPLELQNLKQKLRSCFLEHLDTSIGDVVNELQRLVAEIGSLSSMARTQDEWPELVLSLSASGKDVGVCRLNAKLFLNLRNQDADQDQETLCWRLKSFSFKSAGCSHTCPNCGCTTAIVSGCLSIVVERERKRFLAVVNKEWANIEPMLWQLNPEQTHFLCHVYIHQAKVRPGGEKKNICDSHLRVLFADQACETYTSPGTLSPIWNAVITFNWVSLPGGIGLYLKNPPLLSLEFYNSERSLPEDLVGMGSVAMSVISEDRASDSNWEDAGVFGCSKNPLRKLQRLKYLTPPPLKWVPIARKGTLQAEVLMSAEIIQISMESLTTGQNDKKEPQLATGIPMAIRPTMQNFVLEVFFVGLRNYSKSSMSSAGKRKINLMMGDLVLSSGPSTARIRNSINFLVAYASGVVSLPDQQDYWPAIIATDVLLSGFSSESILGAALIPNSSNFLQRDRTLKCVFKKEQEAAEASTTVSVDEDDEDEEEELEWEEEETKPSRISSWWMRLLFAFGLRPAPYANRHALRFHDDLDDHELVEEREFTWWTKFYNSMYWSAAEMCHEHKHRLVIYYEELEKQAQFGYLQDWAVPVQLVHGVKFKKHGPPKEDIYATLKLQLKLTPCQCPVLEDPGGGGDMVRNMSNPIHPRHQSTLQSLGETVKLLVRVYVVQGVQMRPRDVKGDSDCYVKLFLGGKTFSDRAHFSPNHSNPVFGRLFEMEATLPGDHMLQVMVYDHDKIKDEVIGQTNIDLEDRWRTRHRATVGLANEYTKSGYNHWHDVKLPSEILIDLCQKRGIQAPYYYGNVIEVDGMLFGDETLISKDEELQERLSLAVLKNMDKLPSFGYKLVPEHVETRSLCRDDFPNVEQGKLQLWIELFEANIYVPSPIDITPVPPADFEVRVVVKNLVGIQAGDKNIFGKLMSDVYVIGWCEDEDKRQSTDIHYRSFAGDAAFNWRMVFGLKYSPNEDLMVIRRKAGLLEEIEFKKPPIIYLQVWDKDVLTKDEYLAALELNLSNMYAPYPAAQLCKPYPKKRKRINLFKRKVINGWFPLQSNAHPSQRSQAAVQNGKMQLSIEVCTDVEAVNRPAGRGQDPPMALEPPYRPDSSFNPLTHPCKSFQHILWPVIRKYVLWTLLILIVILGLVLFFSNLPAKLMTIPLE

Target ORF information:

RefSeq Version	NM_001144448.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster misfire (mfr), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144448.2

ORF Insert Sequence:

ATGTTTCCGT TTCTAAAGTA CAGATTCCAT CCTTTCAAGG GCAAAACGCC GGTGGTCAAG 
AACACGACGA CGCCCGTGTG GAACTACGAG ATCAACTTTG CCTGGATGTA TCCGTCGGTG 
GCCCAGCGAT TCCTCATCCT AATTTTTGCC CATGAACATC TGCAATGGAA ATGCGTGGCT 
GAGTTTGAAT TGTGTCTTGA GGAGATCGCG TTTAATGGAA CTCCTTCTCT GGGACCCACC 
TACTTGCATC TCTACGATCC TGTCAATCCT ATGATCTATG TTGGTCGTCT TCTAATGGAA 
CTTCGGTCTG AAATGCTAAC GGATTCCCAG CCCACACACA CGCTGTCCTC CAAGACGGTG 
CCAGGTTTGG ATGAGACCCG TTTCTGGCAT GACGATGTCT ATCTGATTGA GTTTCTTCCA 
CTAATGGGTC ACCACATCCT GACCTCGGGT ACGGCATACA AGATCTCGGC GAGATTAGCC 
GAACACACCT CGAACGTGCT GGAGGGTCAA CTAAGGACAC CTTTAGATCG ATTCAAGTCG 
GCAATGAACT CAAAGAATTT CCGGCGGGAG GCACCTTTTC AGTCCTGCAT GTTTCGTGTC 
CGTTTGCCGG ACAATCGAGC CAAGTACGAA AGTGATTTCT TCATGCTGGA TATAGTCAAT 
TATATGCGTT CAGAACTGGA CACCTTCAAG CTCTTTCAAC GCAAATATCC ACTCCAGGAT 
GATGAGCAGG CCAAATGCCT GAAGGTCATT GTGAACAATA TTTTGACCAG AATCAAGGAG 
GGTCTAGATG CCCACCGTTT CGACCACGAC GTGCAACCAC AGCGCACCAC CTGGGATATC 
AATCGGCAGC TGTACCTCCT GGACTATTTC GCCAAGTTGC CACTGGAACT TCAAAATCTT 
AAACAGAAAC TACGCAGCTG CTTTCTGGAA CACCTAGACA CTTCCATTGG CGATGTGGTT 
AATGAGCTAC AGCGTTTGGT AGCTGAAATC GGATCCCTTT CCAGTATGGC ACGCACCCAG 
GACGAGTGGC CAGAATTGGT GCTTTCCTTA AGTGCAAGCG GTAAAGATGT GGGTGTTTGC 
CGATTGAACG CCAAACTCTT TTTGAATCTA CGAAATCAGG ATGCGGATCA GGATCAGGAG 
ACCCTTTGTT GGCGTCTAAA GAGCTTTTCC TTCAAGTCCG CGGGCTGTTC GCACACCTGC 
CCCAATTGTG GTTGCACCAC GGCCATAGTA TCAGGATGTT TATCCATTGT TGTGGAACGG 
GAGCGAAAAC GATTTCTTGC GGTTGTCAAT AAAGAGTGGG CCAACATTGA GCCCATGTTG 
TGGCAGCTGA ATCCTGAACA GACACACTTC CTCTGTCACG TTTACATACA CCAAGCCAAA 
GTTCGTCCTG GTGGTGAAAA AAAGAACATA TGTGACTCAC ATCTGAGAGT CCTGTTTGCC 
GACCAGGCAT GTGAAACGTA TACGTCTCCC GGTACCCTAT CGCCAATTTG GAATGCTGTG 
ATCACATTTA ACTGGGTGTC ACTACCAGGA GGCATTGGTC TCTATCTAAA GAACCCTCCT 
CTACTTTCCT TGGAATTCTA TAATTCGGAA CGCAGTCTAC CAGAAGACCT TGTGGGCATG 
GGAAGCGTGG CCATGAGTGT TATTAGCGAG GATCGGGCAA GTGATTCCAA TTGGGAGGAT 
GCAGGAGTAT TCGGTTGCTC AAAGAATCCG TTGCGAAAAC TACAGAGATT GAAGTACCTG 
ACACCACCTC CTCTCAAATG GGTGCCAATT GCTAGAAAAG GAACTCTACA AGCAGAGGTT 
TTGATGTCAG CCGAGATCAT TCAAATCTCC ATGGAATCTC TGACCACTGG TCAGAATGAT 
AAAAAGGAAC CACAACTTGC AACGGGCATT CCAATGGCCA TCAGGCCAAC TATGCAGAAT 
TTTGTCTTAG AGGTTTTCTT CGTGGGTCTA CGCAATTACT CCAAGAGCAG CATGAGTTCT 
GCTGGTAAGC GGAAAATCAA TTTAATGATG GGTGATCTGG TCCTGTCCAG TGGACCCTCC 
ACAGCACGCA TCCGGAACAG CATAAACTTT TTGGTGGCCT ACGCATCTGG AGTGGTTAGT 
TTGCCAGATC AACAGGATTA CTGGCCGGCG ATCATAGCCA CCGATGTCCT TCTCTCCGGA 
TTTAGCAGCG AGTCCATTCT GGGTGCAGCT CTCATACCCA ATTCATCAAA TTTCCTGCAA 
CGCGACAGGA CCCTCAAGTG TGTGTTCAAA AAGGAACAAG AGGCAGCAGA AGCTTCCACC 
ACGGTTAGTG TGGATGAAGA TGATGAGGAT GAGGAAGAGG AACTGGAGTG GGAAGAGGAG 
GAGACCAAAC CCTCCAGGAT TAGCAGCTGG TGGATGCGAC TGCTCTTCGC GTTCGGTCTG 
CGTCCAGCCC CCTATGCCAA TCGACACGCT TTAAGGTTCC ACGATGATTT GGATGACCAC 
GAATTGGTCG AGGAGCGAGA GTTCACCTGG TGGACTAAGT TCTACAACTC GATGTATTGG 
AGTGCGGCCG AAATGTGCCA CGAACACAAG CACCGCCTGG TTATCTACTA CGAAGAGCTG 
GAGAAGCAGG CCCAGTTTGG ATACCTCCAG GATTGGGCGG TTCCCGTTCA GTTGGTTCAT 
GGCGTGAAGT TTAAAAAGCA TGGTCCACCC AAAGAAGATA TCTATGCCAC CTTAAAGCTG 
CAACTCAAGT TGACCCCTTG TCAGTGCCCG GTTCTTGAGG ATCCCGGAGG TGGTGGCGAC 
ATGGTTCGCA ACATGTCAAA TCCCATACAT CCTCGACATC AGTCCACACT ACAGTCGCTG 
GGTGAGACCG TAAAACTATT GGTACGTGTT TATGTAGTCC AGGGGGTTCA GATGCGTCCT 
CGAGATGTGA AAGGTGACTC CGATTGCTAT GTGAAGCTGT TCCTGGGCGG CAAGACGTTC 
TCCGATAGAG CCCATTTCTC ACCCAACCAC AGTAATCCAG TTTTCGGTCG TCTATTTGAA 
ATGGAGGCTA CGCTGCCAGG AGATCATATG CTGCAAGTCA TGGTATATGA TCATGACAAG 
ATCAAGGACG AGGTGATCGG TCAGACAAAC ATCGATCTGG AGGATCGCTG GCGAACTAGG 
CATCGTGCCA CAGTGGGTTT GGCCAATGAG TACACAAAGA GTGGCTATAA TCATTGGCAC 
GACGTTAAGT TGCCCTCGGA AATTCTCATA GATCTGTGCC AGAAAAGAGG CATTCAGGCC 
CCTTACTATT ATGGTAATGT AATTGAGGTG GACGGAATGC TTTTTGGGGA CGAAACTTTG 
ATCTCAAAAG ATGAGGAGCT GCAGGAGAGA CTTAGTTTAG CCGTGCTTAA GAATATGGAT 
AAGTTGCCAT CCTTTGGTTA CAAATTGGTT CCCGAACATG TGGAAACCCG CTCCCTTTGC 
CGCGATGATT TTCCAAATGT CGAACAGGGC AAACTACAAC TGTGGATAGA GCTGTTCGAG 
GCGAACATCT ATGTGCCCAG TCCCATCGAT ATAACCCCTG TGCCGCCCGC GGATTTCGAG 
GTGCGTGTTG TGGTTAAGAA TCTGGTCGGG ATTCAGGCGG GCGACAAAAA CATTTTTGGC 
AAACTAATGA GCGACGTTTA CGTGATAGGA TGGTGCGAGG ATGAGGACAA GCGCCAATCG 
ACCGATATCC ATTACCGCTC ATTTGCAGGA GATGCGGCAT TTAATTGGCG CATGGTCTTC 
GGCCTCAAGT ATTCCCCCAA CGAGGACCTG ATGGTAATCC GGCGAAAGGC GGGGCTGCTG 
GAGGAAATCG AGTTCAAGAA GCCGCCTATT ATCTATCTGC AGGTGTGGGA CAAGGATGTG 
TTAACCAAGG ATGAGTATCT GGCCGCACTG GAGCTCAATC TCTCCAACAT GTACGCACCG 
TATCCAGCTG CCCAGTTATG TAAGCCATAT CCCAAGAAGA GGAAGCGCAT CAATCTCTTC 
AAGCGCAAGG TCATCAACGG CTGGTTTCCG CTGCAAAGTA ATGCACATCC ATCGCAACGC 
AGCCAGGCCG CAGTTCAAAA TGGCAAAATG CAGCTGAGCA TCGAAGTCTG CACGGATGTG 
GAGGCCGTCA ACCGGCCGGC TGGTCGTGGA CAGGATCCAC CGATGGCCTT GGAGCCGCCT 
TACAGACCGG ACAGCTCGTT CAATCCACTA ACGCATCCTT GCAAATCCTT TCAGCACATC 
CTCTGGCCAG TCATTCGAAA GTATGTCCTG TGGACTTTGC TCATATTGAT CGTTATCCTG 
GGATTGGTGC TCTTCTTTTC CAATCTGCCC GCCAAACTCA TGACCATTCC ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mfr cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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