mfr cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following mfr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mfr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa17951 NM_001144446.2
Latest version!
Drosophila melanogaster misfire (mfr), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa17956 NM_001144449.2
Latest version!
Drosophila melanogaster misfire (mfr), transcript variant J, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa17953 NM_001144443.2
Latest version!
Drosophila melanogaster misfire (mfr), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa17950 NM_001144444.2
Latest version!
Drosophila melanogaster misfire (mfr), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa17952 NM_001144447.2
Latest version!
Drosophila melanogaster misfire (mfr), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OFa17954 NM_001144445.2
Latest version!
Drosophila melanogaster misfire (mfr), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa17955 NM_001144448.2
Latest version!
Drosophila melanogaster misfire (mfr), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa17951
    Clone ID Related Accession (Same CDS sequence) NM_001144446.2
    Accession Version NM_001144446.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1920bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product misfire, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001144446.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGATCTATG TTGGTCGTCT TCTAATGGAA CTTCGGTCTG AAATGCTAAC GGATTCCCAG 
    CCCACACACA CGCTGTCCTC CAAGACGGTG CCAGGTTTGG ATGAGACCCG TTTCTGGCAT
    GACGATGTCT ATCTGATTGA GTTTCTTCCA CTAATGGGTC ACCACATCCT GACCTCGGGT
    ACGGCATACA AGATCTCGGC GAGATTAGCC GAACACACCT CGAACGTGCT GGAGGGTCAA
    CTAAGGACAC CTTTAGATCG ATTCAAGTCG GCAATGAACT CAAAGAATTT CCGGCGGGAG
    GCACCTTTTC AGTCCTGCAT GTTTCGTGTC CGTTTGCCGG ACAATCGAGC CAAGTACGAA
    AGTGATTTCT TCATGCTGGA TATAGTCAAT TATATGCGTT CAGAACTGGA CACCTTCAAG
    CTCTTTCAAC GCAAATATCC ACTCCAGGAT GATGAGCAGG CCAAATGCCT GAAGGTCATT
    GTGAACAATA TTTTGACCAG AATCAAGGAG GGTCTAGATG CCCACCGTTT CGACCACGAC
    GTGCAACCAC AGCGCACCAC CTGGGATATC AATCGGCAGC TGTACCTCCT GGACTATTTC
    GCCAAGTTGC CACTGGAACT TCAAAATCTT AAACAGAAAC TACGCAGCTG CTTTCTGGAA
    CACCTAGACA CTTCCATTGG CGATGTGGTT AATGAGCTAC AGCGTTTGGT AGCTGAAATC
    GGATCCCTTT CCAGTATGGC ACGCACCCAG GACGAGTGGC CAGAATTGGT GCTTTCCTTA
    AGTGCAAGCG GTAAAGATGT GGGTGTTTGC CGATTGAACG CCAAACTCTT TTTGAATCTA
    CGAAATCAGG ATGCGGATCA GGATCAGGAG ACCCTTTGTT GGCGTCTAAA GAGCTTTTCC
    TTCAAGTCCG CGGGCTGTTC GCACACCTGC CCCAATTGTG GTTGCACCAC GGCCATAGTA
    TCAGGATGTT TATCCATTGT TGTGGAACGG GAGCGAAAAC GATTTCTTGC GGTTGTCAAT
    AAAGAGTGGG CCAACATTGA GCCCATGTTG TGGCAGCTGA ATCCTGAACA GACACACTTC
    CTCTGTCACG TTTACATACA CCAAGCCAAA GTTCGTCCTG GTGGTGAAAA AAAGAACATA
    TGTGACTCAC ATCTGAGAGT CCTGTTTGCC GACCAGGCAT GTGAAACGTA TACGTCTCCC
    GGTACCCTAT CGCCAATTTG GAATGCTGTG ATCACATTTA ACTGGGTGTC ACTACCAGGA
    GGCATTGGTC TCTATCTAAA GAACCCTCCT CTACTTTCCT TGGAATTCTA TAATTCGGAA
    CGCAGTCTAC CAGAAGACCT TGTGGGCATG GGAAGCGTGG CCATGAGTGT TATTAGCGAG
    GATCGGGCAA GTGATTCCAA TTGGGAGGAT GCAGGAGTAT TCGGTTGCTC AAAGAATCCG
    TTGCGAAAAC TACAGAGATT GAAGTACCTG ACACCACCTC CTCTCAAATG GGTGCCAATT
    GCTAGAAAAG GAACTCTACA AGCAGAGGTT TTGATGTCAG CCGAGATCAT TCAAATCTCC
    ATGGAATCTC TGACCACTGG TCAGAATGAT AAAAAGGAAC CACAACTTGC AACGGGCATT
    CCAATGGCCA TCAGGCCAAC TATGCAGAAT TTTGTCTTAG AGGTTTTCTT CGTGGGTCTA
    CGCAATTACT CCAAGAGCAG CATGAGTTCT GCTGGTAAGC GGAAAATCAA TTTAATGATG
    GGTGATCTGG TCCTGTCCAG TGGACCCTCC ACAGCACGCA TCCGGAACAG CATAAACTTT
    TTGGTGGCCT ACGCATCTGG AGTGGTTGTA AGTTCATATC GTAGAAAAGT AAGGTCCCAC
    CTATATAGTA CCCCGATCAG AGTTTGCCAG ATCAACAGGA TTACTGGCCG GCGATCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137918.1
    CDS1433..3352
    Translation

    Target ORF information:

    RefSeq Version NM_001144446.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster misfire (mfr), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144446.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGATCTATG TTGGTCGTCT TCTAATGGAA CTTCGGTCTG AAATGCTAAC GGATTCCCAG 
    CCCACACACA CGCTGTCCTC CAAGACGGTG CCAGGTTTGG ATGAGACCCG TTTCTGGCAT
    GACGATGTCT ATCTGATTGA GTTTCTTCCA CTAATGGGTC ACCACATCCT GACCTCGGGT
    ACGGCATACA AGATCTCGGC GAGATTAGCC GAACACACCT CGAACGTGCT GGAGGGTCAA
    CTAAGGACAC CTTTAGATCG ATTCAAGTCG GCAATGAACT CAAAGAATTT CCGGCGGGAG
    GCACCTTTTC AGTCCTGCAT GTTTCGTGTC CGTTTGCCGG ACAATCGAGC CAAGTACGAA
    AGTGATTTCT TCATGCTGGA TATAGTCAAT TATATGCGTT CAGAACTGGA CACCTTCAAG
    CTCTTTCAAC GCAAATATCC ACTCCAGGAT GATGAGCAGG CCAAATGCCT GAAGGTCATT
    GTGAACAATA TTTTGACCAG AATCAAGGAG GGTCTAGATG CCCACCGTTT CGACCACGAC
    GTGCAACCAC AGCGCACCAC CTGGGATATC AATCGGCAGC TGTACCTCCT GGACTATTTC
    GCCAAGTTGC CACTGGAACT TCAAAATCTT AAACAGAAAC TACGCAGCTG CTTTCTGGAA
    CACCTAGACA CTTCCATTGG CGATGTGGTT AATGAGCTAC AGCGTTTGGT AGCTGAAATC
    GGATCCCTTT CCAGTATGGC ACGCACCCAG GACGAGTGGC CAGAATTGGT GCTTTCCTTA
    AGTGCAAGCG GTAAAGATGT GGGTGTTTGC CGATTGAACG CCAAACTCTT TTTGAATCTA
    CGAAATCAGG ATGCGGATCA GGATCAGGAG ACCCTTTGTT GGCGTCTAAA GAGCTTTTCC
    TTCAAGTCCG CGGGCTGTTC GCACACCTGC CCCAATTGTG GTTGCACCAC GGCCATAGTA
    TCAGGATGTT TATCCATTGT TGTGGAACGG GAGCGAAAAC GATTTCTTGC GGTTGTCAAT
    AAAGAGTGGG CCAACATTGA GCCCATGTTG TGGCAGCTGA ATCCTGAACA GACACACTTC
    CTCTGTCACG TTTACATACA CCAAGCCAAA GTTCGTCCTG GTGGTGAAAA AAAGAACATA
    TGTGACTCAC ATCTGAGAGT CCTGTTTGCC GACCAGGCAT GTGAAACGTA TACGTCTCCC
    GGTACCCTAT CGCCAATTTG GAATGCTGTG ATCACATTTA ACTGGGTGTC ACTACCAGGA
    GGCATTGGTC TCTATCTAAA GAACCCTCCT CTACTTTCCT TGGAATTCTA TAATTCGGAA
    CGCAGTCTAC CAGAAGACCT TGTGGGCATG GGAAGCGTGG CCATGAGTGT TATTAGCGAG
    GATCGGGCAA GTGATTCCAA TTGGGAGGAT GCAGGAGTAT TCGGTTGCTC AAAGAATCCG
    TTGCGAAAAC TACAGAGATT GAAGTACCTG ACACCACCTC CTCTCAAATG GGTGCCAATT
    GCTAGAAAAG GAACTCTACA AGCAGAGGTT TTGATGTCAG CCGAGATCAT TCAAATCTCC
    ATGGAATCTC TGACCACTGG TCAGAATGAT AAAAAGGAAC CACAACTTGC AACGGGCATT
    CCAATGGCCA TCAGGCCAAC TATGCAGAAT TTTGTCTTAG AGGTTTTCTT CGTGGGTCTA
    CGCAATTACT CCAAGAGCAG CATGAGTTCT GCTGGTAAGC GGAAAATCAA TTTAATGATG
    GGTGATCTGG TCCTGTCCAG TGGACCCTCC ACAGCACGCA TCCGGAACAG CATAAACTTT
    TTGGTGGCCT ACGCATCTGG AGTGGTTGTA AGTTCATATC GTAGAAAAGT AAGGTCCCAC
    CTATATAGTA CCCCGATCAG AGTTTGCCAG ATCAACAGGA TTACTGGCCG GCGATCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa17956
    Clone ID Related Accession (Same CDS sequence) NM_001144449.2
    Accession Version NM_001144449.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product misfire, isoform J
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001144449.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCGACTGC TCTTCGCGTT CGGTCTGCGT CCAGCCCCCT ATGCCAATCG ACACGCTTTA 
    AGGTTCCACG ATGATTTGGA TGACCACGAA TTGGTCGAGG AGCGAGAGTT CACCTGGTGG
    ACTAAGTTCT ACAACTCGAT GTATTGGAGT GCGGCCGAAA TGTGCCACGA ACACAAGCAC
    CGCCTGGTTA TCTACTACGA AGAGCTGGAG AAGCAGGCCC AGTTTGGATA CCTCCAGGAT
    TGGGCGGTTC CCGTTCAGTT GGTTCATGGC GTGAAGTTTA AAAAGCATGG TCCACCCAAA
    GAAGATATCT ATGCCACCTT AAAGCTGCAA CTCAAGTTGA CCCCTTGTCA GTGCCCGGTT
    CTTGAGGATC CCGGAGGTGG TGGCGACATG GTTCGCAACA TGTCAAATCC CATACATCCT
    CGACATCAGT CCACACTACA GTCGCTGGGT GAGACCGTAA AACTATTGGT ACGTGTTTAT
    GTAGTCCAGG GGGTTCAGAT GCGTCCTCGA GATGTGAAAG GTGACTCCGA TTGCTATGTG
    AAGCTGTTCC TGGGCGGCAA GACGTTCTCC GATAGAGCCC ATTTCTCACC CAACCACAGT
    AATCCAGTTT TCGGTCGTCT ATTTGAAATG GAGGCTACGC TGCCAGGAGA TCATATGCTG
    CAAGTCATGG TATATGATCA TGACAAGATC AAGGACGAGG TGATCGGTCA GACAAACATC
    GATCTGGAGG ATCGCTGGCG AACTAGGCAT CGTGCCACAG TGGGTTTGGC CAATGAGTAC
    ACAAAGAGTG GCTATAATCA TTGGCACGAC GTTAAGTTGC CCTCGGAAAT TCTCATAGAT
    CTGTGCCAGA AAAGAGGCAT TCAGGCCCCT TACTATTATG GTAATGTAAT TGAGGTGGAC
    GGAATGCTTT TTGGGGACGA AACTTTGATC TCAAAAGATG AGGAGCTGCA GGAGAGACTT
    AGTTTAGCCG TGCTTAAGAA TATGGATAAG TTGCCATCCT TTGGTTACAA ATTGGTTCCC
    GAACATGTGG AAACCCGCTC CCTTTGCCGC GATGATTTTC CAAATGTCGA ACAGGGCAAA
    CTACAACTGT GGATAGAGCT GTTCGAGGCG AACATCTATG TGCCCAGTCC CATCGATATA
    ACCCCTGTGC CGCCCGCGGA TTTCGAGGTG CGTGTTGTGG TTAAGAATCT GGTCGGGATT
    CAGGCGGGCG ACAAAAACAT TTTTGGCAAA CTAATGAGCG ACGTTTACGT GATAGGATGG
    TGCGAGGATG AGGACAAGCG CCAATCGACC GATATCCATT ACCGCTCATT TGCAGGAGAT
    GCGGCATTTA ATTGGCGCAT GGTCTTCGGC CTCAAGTATT CCCCCAACGA GGACCTGATG
    GTAATCCGGC GAAAGGCGGG GCTGCTGGAG GAAATCGAGT TCAAGAAGCC GCCTATTATC
    TATCTGCAGG TGTGGGACAA GGATGTGTTA ACCAAGGATG AGTATCTGGC CGCACTGGAG
    CTCAATCTCT CCAACATGTA CGCACCGTAT CCAGCTGCCC AGTTATGTAA GCCATATCCC
    AAGAAGAGGA AGCGCATCAA TCTCTTCAAG CGCAAGGTCA TCAACGGCTG GTTTCCGCTG
    CAAAGTAATG CACATCCATC GCAACGCAGC CAGGCCGCAG TTCAAAATGG CAAAATGCAG
    CTGAGCATCG AAGTCTGCAC GGATGTGGAG GCCGTCAACC GGCCGGCTGG TCGTGGACAG
    GATCCACCGA TGGCCTTGGA GCCGCCTTAC AGACCGGACA GCTCGTTCAA TCCACTAACG
    CATCCTTGCA AATCCTTTCA GCACATCCTC TGGCCAGTCA TTCGAAAGTA TGTCCTGTGG
    ACTTTGCTCA TATTGATCGT TATCCTGGGA TTGGTGCTCT TCTTTTCCAA TCTGCCCGCC
    AAACTCATGA CCATTCCACT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137921.1
    CDS3037..4983
    Translation

    Target ORF information:

    RefSeq Version NM_001144449.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster misfire (mfr), transcript variant J, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144449.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCGACTGC TCTTCGCGTT CGGTCTGCGT CCAGCCCCCT ATGCCAATCG ACACGCTTTA 
    AGGTTCCACG ATGATTTGGA TGACCACGAA TTGGTCGAGG AGCGAGAGTT CACCTGGTGG
    ACTAAGTTCT ACAACTCGAT GTATTGGAGT GCGGCCGAAA TGTGCCACGA ACACAAGCAC
    CGCCTGGTTA TCTACTACGA AGAGCTGGAG AAGCAGGCCC AGTTTGGATA CCTCCAGGAT
    TGGGCGGTTC CCGTTCAGTT GGTTCATGGC GTGAAGTTTA AAAAGCATGG TCCACCCAAA
    GAAGATATCT ATGCCACCTT AAAGCTGCAA CTCAAGTTGA CCCCTTGTCA GTGCCCGGTT
    CTTGAGGATC CCGGAGGTGG TGGCGACATG GTTCGCAACA TGTCAAATCC CATACATCCT
    CGACATCAGT CCACACTACA GTCGCTGGGT GAGACCGTAA AACTATTGGT ACGTGTTTAT
    GTAGTCCAGG GGGTTCAGAT GCGTCCTCGA GATGTGAAAG GTGACTCCGA TTGCTATGTG
    AAGCTGTTCC TGGGCGGCAA GACGTTCTCC GATAGAGCCC ATTTCTCACC CAACCACAGT
    AATCCAGTTT TCGGTCGTCT ATTTGAAATG GAGGCTACGC TGCCAGGAGA TCATATGCTG
    CAAGTCATGG TATATGATCA TGACAAGATC AAGGACGAGG TGATCGGTCA GACAAACATC
    GATCTGGAGG ATCGCTGGCG AACTAGGCAT CGTGCCACAG TGGGTTTGGC CAATGAGTAC
    ACAAAGAGTG GCTATAATCA TTGGCACGAC GTTAAGTTGC CCTCGGAAAT TCTCATAGAT
    CTGTGCCAGA AAAGAGGCAT TCAGGCCCCT TACTATTATG GTAATGTAAT TGAGGTGGAC
    GGAATGCTTT TTGGGGACGA AACTTTGATC TCAAAAGATG AGGAGCTGCA GGAGAGACTT
    AGTTTAGCCG TGCTTAAGAA TATGGATAAG TTGCCATCCT TTGGTTACAA ATTGGTTCCC
    GAACATGTGG AAACCCGCTC CCTTTGCCGC GATGATTTTC CAAATGTCGA ACAGGGCAAA
    CTACAACTGT GGATAGAGCT GTTCGAGGCG AACATCTATG TGCCCAGTCC CATCGATATA
    ACCCCTGTGC CGCCCGCGGA TTTCGAGGTG CGTGTTGTGG TTAAGAATCT GGTCGGGATT
    CAGGCGGGCG ACAAAAACAT TTTTGGCAAA CTAATGAGCG ACGTTTACGT GATAGGATGG
    TGCGAGGATG AGGACAAGCG CCAATCGACC GATATCCATT ACCGCTCATT TGCAGGAGAT
    GCGGCATTTA ATTGGCGCAT GGTCTTCGGC CTCAAGTATT CCCCCAACGA GGACCTGATG
    GTAATCCGGC GAAAGGCGGG GCTGCTGGAG GAAATCGAGT TCAAGAAGCC GCCTATTATC
    TATCTGCAGG TGTGGGACAA GGATGTGTTA ACCAAGGATG AGTATCTGGC CGCACTGGAG
    CTCAATCTCT CCAACATGTA CGCACCGTAT CCAGCTGCCC AGTTATGTAA GCCATATCCC
    AAGAAGAGGA AGCGCATCAA TCTCTTCAAG CGCAAGGTCA TCAACGGCTG GTTTCCGCTG
    CAAAGTAATG CACATCCATC GCAACGCAGC CAGGCCGCAG TTCAAAATGG CAAAATGCAG
    CTGAGCATCG AAGTCTGCAC GGATGTGGAG GCCGTCAACC GGCCGGCTGG TCGTGGACAG
    GATCCACCGA TGGCCTTGGA GCCGCCTTAC AGACCGGACA GCTCGTTCAA TCCACTAACG
    CATCCTTGCA AATCCTTTCA GCACATCCTC TGGCCAGTCA TTCGAAAGTA TGTCCTGTGG
    ACTTTGCTCA TATTGATCGT TATCCTGGGA TTGGTGCTCT TCTTTTCCAA TCTGCCCGCC
    AAACTCATGA CCATTCCACT TGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa17953
    Clone ID Related Accession (Same CDS sequence) NM_001144443.2
    Accession Version NM_001144443.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3138bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product misfire, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001144443.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCACGCA CCCAGGACGA GTGGCCAGAA TTGGTGCTTT CCTTAAGTGC AAGCGGTAAA 
    GATGTGGGTG TTTGCCGATT GAACGCCAAA CTCTTTTTGA ATCTACGAAA TCAGGATGCG
    GATCAGGATC AGGAGACCCT TTGTTGGCGT CTAAAGAGCT TTTCCTTCAA GTCCGCGGGC
    TGTTCGCACA CCTGCCCCAA TTGTGGTTGC ACCACGGCCA TAGTATCAGG ATGTTTATCC
    ATTGTTGTGG AACGGGAGCG AAAACGATTT CTTGCGGTTG TCAATAAAGA GTGGGCCAAC
    ATTGAGCCCA TGTTGTGGCA GCTGAATCCT GAACAGACAC ACTTCCTCTG TCACGTTTAC
    ATACACCAAG CCAAAGTTCG TCCTGGTGGT GAAAAAAAGA ACATATGTGA CTCACATCTG
    AGAGTCCTGT TTGCCGACCA GGCATGTGAA ACGTATACGT CTCCCGGTAC CCTATCGCCA
    ATTTGGAATG CTGTGATCAC ATTTAACTGG GTGTCACTAC CAGGAGGCAT TGGTCTCTAT
    CTAAAGAACC CTCCTCTACT TTCCTTGGAA TTCTATAATT CGGAACGCAG TCTACCAGAA
    GACCTTGTGG GCATGGGAAG CGTGGCCATG AGTGTTATTA GCGAGGATCG GGCAAGTGAT
    TCCAATTGGG AGGATGCAGG AGTATTCGGT TGCTCAAAGA ATCCGTTGCG AAAACTACAG
    AGATTGAAGT ACCTGACACC ACCTCCTCTC AAATGGGTGC CAATTGCTAG AAAAGGAACT
    CTACAAGCAG AGGTTTTGAT GTCAGCCGAG ATCATTCAAA TCTCCATGGA ATCTCTGACC
    ACTGGTCAGA ATGATAAAAA GGAACCACAA CTTGCAACGG GCATTCCAAT GGCCATCAGG
    CCAACTATGC AGAATTTTGT CTTAGAGGTT TTCTTCGTGG GTCTACGCAA TTACTCCAAG
    AGCAGCATGA GTTCTGCTGG TAAGCGGAAA ATCAATTTAA TGATGGGTGA TCTGGTCCTG
    TCCAGTGGAC CCTCCACAGC ACGCATCCGG AACAGCATAA ACTTTTTGGT GGCCTACGCA
    TCTGGAGTGG TTAGTTTGCC AGATCAACAG GATTACTGGC CGGCGATCAT AGCCACCGAT
    GTCCTTCTCT CCGGATTTAG CAGCGAGTCC ATTCTGGGTG CAGCTCTCAT ACCCAATTCA
    TCAAATTTCC TGCAACGCGA CAGGACCCTC AAGTGTGTGT TCAAAAAGGA ACAAGAGGCA
    GCAGAAGCTT CCACCACGGT TAGTGTGGAT GAAGATGATG AGGATGAGGA AGAGGAACTG
    GAGTGGGAAG AGGAGGAGAC CAAACCCTCC AGGATTAGCA GCTGGTGGAT GCGACTGCTC
    TTCGCGTTCG GTCTGCGTCC AGCCCCCTAT GCCAATCGAC ACGCTTTAAG GTTCCACGAT
    GATTTGGATG ACCACGAATT GGTCGAGGAG CGAGAGTTCA CCTGGTGGAC TAAGTTCTAC
    AACTCGATGT ATTGGAGTGC GGCCGAAATG TGCCACGAAC ACAAGCACCG CCTGGTTATC
    TACTACGAAG AGCTGGAGAA GCAGGCCCAG TTTGGATACC TCCAGGATTG GGCGGTTCCC
    GTTCAGTTGG TTCATGGCGT GAAGTTTAAA AAGCATGGTC CACCCAAAGA AGATATCTAT
    GCCACCTTAA AGCTGCAACT CAAGTTGACC CCTTGTCAGT GCCCGGTTCT TGAGGATCCC
    GGAGGTGGTG GCGACATGGT TCGCAACATG TCAAATCCCA TACATCCTCG ACATCAGTCC
    ACACTACAGT CGCTGGGTGA GACCGTAAAA CTATTGGTAC GTGTTTATGT AGTCCAGGGG
    GTTCAGATGC GTCCTCGAGA TGTGAAAGGT GACTCCGATT GCTATGTGAA GCTGTTCCTG
    GGCGGCAAGA CGTTCTCCGA TAGAGCCCAT TTCTCACCCA ACCACAGTAA TCCAGTTTTC
    GGTCGTCTAT TTGAAATGGA GGCTACGCTG CCAGGAGATC ATATGCTGCA AGTCATGGTA
    TATGATCATG ACAAGATCAA GGACGAGGTG ATCGGTCAGA CAAACATCGA TCTGGAGGAT
    CGCTGGCGAA CTAGGCATCG TGCCACAGTG GGTTTGGCCA ATGAGTACAC AAAGAGTGGC
    TATAATCATT GGCACGACGT TAAGTTGCCC TCGGAAATTC TCATAGATCT GTGCCAGAAA
    AGAGGCATTC AGGCCCCTTA CTATTATGGT AATGTAATTG AGGTGGACGG AATGCTTTTT
    GGGGACGAAA CTTTGATCTC AAAAGATGAG GAGCTGCAGG AGAGACTTAG TTTAGCCGTG
    CTTAAGAATA TGGATAAGTT GCCATCCTTT GGTTACAAAT TGGTTCCCGA ACATGTGGAA
    ACCCGCTCCC TTTGCCGCGA TGATTTTCCA AATGTCGAAC AGGGCAAACT ACAACTGTGG
    ATAGAGCTGT TCGAGGCGAA CATCTATGTG CCCAGTCCCA TCGATATAAC CCCTGTGCCG
    CCCGCGGATT TCGAGGTGCG TGTTGTGGTT AAGAATCTGG TCGGGATTCA GGCGGGCGAC
    AAAAACATTT TTGGCAAACT AATGAGCGAC GTTTACGTGA TAGGATGGTG CGAGGATGAG
    GACAAGCGCC AATCGACCGA TATCCATTAC CGCTCATTTG CAGGAGATGC GGCATTTAAT
    TGGCGCATGG TCTTCGGCCT CAAGTATTCC CCCAACGAGG ACCTGATGGT AATCCGGCGA
    AAGGCGGGGC TGCTGGAGGA AATCGAGTTC AAGAAGCCGC CTATTATCTA TCTGCAGGTG
    TGGGACAAGG ATGTGTTAAC CAAGGATGAG TATCTGGCCG CACTGGAGCT CAATCTCTCC
    AACATGTACG CACCGTATCC AGCTGCCCAG TTATGTAAGC CATATCCCAA GAAGAGGAAG
    CGCATCAATC TCTTCAAGCG CAAGGTCATC AACGGCTGGT TTCCGCTGCA AAGTAATGCA
    CATCCATCGC AACGCAGCCA GGCCGCAGTT CAAAATGGCA AAATGCAGCT GAGCATCGAA
    GTCTGCACGG ATGTGGAGGC CGTCAACCGG CCGGCTGGTC GTGGACAGGA TCCACCGATG
    GCCTTGGAGC CGCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137915.1
    CDS1733..4870
    Translation

    Target ORF information:

    RefSeq Version NM_001144443.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster misfire (mfr), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144443.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCACGCA CCCAGGACGA GTGGCCAGAA TTGGTGCTTT CCTTAAGTGC AAGCGGTAAA 
    GATGTGGGTG TTTGCCGATT GAACGCCAAA CTCTTTTTGA ATCTACGAAA TCAGGATGCG
    GATCAGGATC AGGAGACCCT TTGTTGGCGT CTAAAGAGCT TTTCCTTCAA GTCCGCGGGC
    TGTTCGCACA CCTGCCCCAA TTGTGGTTGC ACCACGGCCA TAGTATCAGG ATGTTTATCC
    ATTGTTGTGG AACGGGAGCG AAAACGATTT CTTGCGGTTG TCAATAAAGA GTGGGCCAAC
    ATTGAGCCCA TGTTGTGGCA GCTGAATCCT GAACAGACAC ACTTCCTCTG TCACGTTTAC
    ATACACCAAG CCAAAGTTCG TCCTGGTGGT GAAAAAAAGA ACATATGTGA CTCACATCTG
    AGAGTCCTGT TTGCCGACCA GGCATGTGAA ACGTATACGT CTCCCGGTAC CCTATCGCCA
    ATTTGGAATG CTGTGATCAC ATTTAACTGG GTGTCACTAC CAGGAGGCAT TGGTCTCTAT
    CTAAAGAACC CTCCTCTACT TTCCTTGGAA TTCTATAATT CGGAACGCAG TCTACCAGAA
    GACCTTGTGG GCATGGGAAG CGTGGCCATG AGTGTTATTA GCGAGGATCG GGCAAGTGAT
    TCCAATTGGG AGGATGCAGG AGTATTCGGT TGCTCAAAGA ATCCGTTGCG AAAACTACAG
    AGATTGAAGT ACCTGACACC ACCTCCTCTC AAATGGGTGC CAATTGCTAG AAAAGGAACT
    CTACAAGCAG AGGTTTTGAT GTCAGCCGAG ATCATTCAAA TCTCCATGGA ATCTCTGACC
    ACTGGTCAGA ATGATAAAAA GGAACCACAA CTTGCAACGG GCATTCCAAT GGCCATCAGG
    CCAACTATGC AGAATTTTGT CTTAGAGGTT TTCTTCGTGG GTCTACGCAA TTACTCCAAG
    AGCAGCATGA GTTCTGCTGG TAAGCGGAAA ATCAATTTAA TGATGGGTGA TCTGGTCCTG
    TCCAGTGGAC CCTCCACAGC ACGCATCCGG AACAGCATAA ACTTTTTGGT GGCCTACGCA
    TCTGGAGTGG TTAGTTTGCC AGATCAACAG GATTACTGGC CGGCGATCAT AGCCACCGAT
    GTCCTTCTCT CCGGATTTAG CAGCGAGTCC ATTCTGGGTG CAGCTCTCAT ACCCAATTCA
    TCAAATTTCC TGCAACGCGA CAGGACCCTC AAGTGTGTGT TCAAAAAGGA ACAAGAGGCA
    GCAGAAGCTT CCACCACGGT TAGTGTGGAT GAAGATGATG AGGATGAGGA AGAGGAACTG
    GAGTGGGAAG AGGAGGAGAC CAAACCCTCC AGGATTAGCA GCTGGTGGAT GCGACTGCTC
    TTCGCGTTCG GTCTGCGTCC AGCCCCCTAT GCCAATCGAC ACGCTTTAAG GTTCCACGAT
    GATTTGGATG ACCACGAATT GGTCGAGGAG CGAGAGTTCA CCTGGTGGAC TAAGTTCTAC
    AACTCGATGT ATTGGAGTGC GGCCGAAATG TGCCACGAAC ACAAGCACCG CCTGGTTATC
    TACTACGAAG AGCTGGAGAA GCAGGCCCAG TTTGGATACC TCCAGGATTG GGCGGTTCCC
    GTTCAGTTGG TTCATGGCGT GAAGTTTAAA AAGCATGGTC CACCCAAAGA AGATATCTAT
    GCCACCTTAA AGCTGCAACT CAAGTTGACC CCTTGTCAGT GCCCGGTTCT TGAGGATCCC
    GGAGGTGGTG GCGACATGGT TCGCAACATG TCAAATCCCA TACATCCTCG ACATCAGTCC
    ACACTACAGT CGCTGGGTGA GACCGTAAAA CTATTGGTAC GTGTTTATGT AGTCCAGGGG
    GTTCAGATGC GTCCTCGAGA TGTGAAAGGT GACTCCGATT GCTATGTGAA GCTGTTCCTG
    GGCGGCAAGA CGTTCTCCGA TAGAGCCCAT TTCTCACCCA ACCACAGTAA TCCAGTTTTC
    GGTCGTCTAT TTGAAATGGA GGCTACGCTG CCAGGAGATC ATATGCTGCA AGTCATGGTA
    TATGATCATG ACAAGATCAA GGACGAGGTG ATCGGTCAGA CAAACATCGA TCTGGAGGAT
    CGCTGGCGAA CTAGGCATCG TGCCACAGTG GGTTTGGCCA ATGAGTACAC AAAGAGTGGC
    TATAATCATT GGCACGACGT TAAGTTGCCC TCGGAAATTC TCATAGATCT GTGCCAGAAA
    AGAGGCATTC AGGCCCCTTA CTATTATGGT AATGTAATTG AGGTGGACGG AATGCTTTTT
    GGGGACGAAA CTTTGATCTC AAAAGATGAG GAGCTGCAGG AGAGACTTAG TTTAGCCGTG
    CTTAAGAATA TGGATAAGTT GCCATCCTTT GGTTACAAAT TGGTTCCCGA ACATGTGGAA
    ACCCGCTCCC TTTGCCGCGA TGATTTTCCA AATGTCGAAC AGGGCAAACT ACAACTGTGG
    ATAGAGCTGT TCGAGGCGAA CATCTATGTG CCCAGTCCCA TCGATATAAC CCCTGTGCCG
    CCCGCGGATT TCGAGGTGCG TGTTGTGGTT AAGAATCTGG TCGGGATTCA GGCGGGCGAC
    AAAAACATTT TTGGCAAACT AATGAGCGAC GTTTACGTGA TAGGATGGTG CGAGGATGAG
    GACAAGCGCC AATCGACCGA TATCCATTAC CGCTCATTTG CAGGAGATGC GGCATTTAAT
    TGGCGCATGG TCTTCGGCCT CAAGTATTCC CCCAACGAGG ACCTGATGGT AATCCGGCGA
    AAGGCGGGGC TGCTGGAGGA AATCGAGTTC AAGAAGCCGC CTATTATCTA TCTGCAGGTG
    TGGGACAAGG ATGTGTTAAC CAAGGATGAG TATCTGGCCG CACTGGAGCT CAATCTCTCC
    AACATGTACG CACCGTATCC AGCTGCCCAG TTATGTAAGC CATATCCCAA GAAGAGGAAG
    CGCATCAATC TCTTCAAGCG CAAGGTCATC AACGGCTGGT TTCCGCTGCA AAGTAATGCA
    CATCCATCGC AACGCAGCCA GGCCGCAGTT CAAAATGGCA AAATGCAGCT GAGCATCGAA
    GTCTGCACGG ATGTGGAGGC CGTCAACCGG CCGGCTGGTC GTGGACAGGA TCCACCGATG
    GCCTTGGAGC CGCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa17950
    Clone ID Related Accession (Same CDS sequence) NM_001144444.2
    Accession Version NM_001144444.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4197bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product misfire, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001144444.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTTTCCGT TTCTAAAGTA CAGATTCCAT CCTTTCAAGG GCAAAACGCC GGTGGTCAAG 
    AACACGACGA CGCCCGTGTG GAACTACGAG ATCAACTTTG CCTGGATGTA TCCGTCGGTG
    GCCCAGCGAT TCCTCATCCT AATTTTTGCC CATGAACATC TGCAATGGAA ATGCGTGGCT
    GAGTTTGAAT TGTGTCTTGA GGAGATCGCG TTTAATGGAA CTCCTTCTCT GGGACCCACC
    TACTTGCATC TCTACGATCC TGTCAATCCT ATGATCTATG TTGGTCGTCT TCTAATGGAA
    CTTCGGTCTG AAATGCTAAC GGATTCCCAG CCCACACACA CGCTGTCCTC CAAGACGGTG
    CCAGGTTTGG ATGAGACCCG TTTCTGGCAT GACGATGTCT ATCTGATTGA GTTTCTTCCA
    CTAATGGGTC ACCACATCCT GACCTCGGGT ACGGCATACA AGATCTCGGC GAGATTAGCC
    GAACACACCT CGAACGTGCT GGAGGGTCAA CTAAGGACAC CTTTAGATCG ATTCAAGTCG
    GCAATGAACT CAAAGAATTT CCGGCGGGAG GCACCTTTTC AGTCCTGCAT GTTTCGTGTC
    CGTTTGCCGG ACAATCGAGC CAAGTACGAA AGTGATTTCT TCATGCTGGA TATAGTCAAT
    TATATGCGTT CAGAACTGGA CACCTTCAAG CTCTTTCAAC GCAAATATCC ACTCCAGGAT
    GATGAGCAGG CCAAATGCCT GAAGGTCATT GTGAACAATA TTTTGACCAG AATCAAGGAG
    GGTCTAGATG CCCACCGTTT CGACCACGAC GTGCAACCAC AGCGCACCAC CTGGGATATC
    AATCGGCAGC TGTACCTCCT GGACTATTTC GCCAAGTTGC CACTGGAACT TCAAAATCTT
    AAACAGAAAC TACGCAGCTG CTTTCTGGAA CACCTAGACA CTTCCATTGG CGATGTGGTT
    AATGAGCTAC AGCGTTTGGT AGCTGAAATC GGATCCCTTT CCAGTATGGC ACGCACCCAG
    GACGAGTGGC CAGAATTGGT GCTTTCCTTA AGTGCAAGCG GTAAAGATGT GGGTGTTTGC
    CGATTGAACG CCAAACTCTT TTTGAATCTA CGAAATCAGG ATGCGGATCA GGATCAGGAG
    ACCCTTTGTT GGCGTCTAAA GAGCTTTTCC TTCAAGTCCG CGGGCTGTTC GCACACCTGC
    CCCAATTGTG GTTGCACCAC GGCCATAGTA TCAGGATGTT TATCCATTGT TGTGGAACGG
    GAGCGAAAAC GATTTCTTGC GGTTGTCAAT AAAGAGTGGG CCAACATTGA GCCCATGTTG
    TGGCAGCTGA ATCCTGAACA GACACACTTC CTCTGTCACG TTTACATACA CCAAGCCAAA
    GTTCGTCCTG GTGGTGAAAA AAAGAACATA TGTGACTCAC ATCTGAGAGT CCTGTTTGCC
    GACCAGGCAT GTGAAACGTA TACGTCTCCC GGTACCCTAT CGCCAATTTG GAATGCTGTG
    ATCACATTTA ACTGGGTGTC ACTACCAGGA GGCATTGGTC TCTATCTAAA GAACCCTCCT
    CTACTTTCCT TGGAATTCTA TAATTCGGAA CGCAGTCTAC CAGAAGACCT TGTGGGCATG
    GGAAGCGTGG CCATGAGTGT TATTAGCGAG GATCGGGCAA GTGATTCCAA TTGGGAGGAT
    GCAGGAGTAT TCGGTTGCTC AAAGAATCCG TTGCGAAAAC TACAGAGATT GAAGTACCTG
    ACACCACCTC CTCTCAAATG GGTGCCAATT GCTAGAAAAG GAACTCTACA AGCAGAGGTT
    TTGATGTCAG CCGAGATCAT TCAAATCTCC ATGGAATCTC TGACCACTGG TCAGAATGAT
    AAAAAGGAAC CACAACTTGC AACGGGCATT CCAATGGCCA TCAGGCCAAC TATGCAGAAT
    TTTGTCTTAG AGGTTTTCTT CGTGGGTCTA CGCAATTACT CCAAGAGCAG CATGAGTTCT
    GCTGGTAAGC GGAAAATCAA TTTAATGATG GGTGATCTGG TCCTGTCCAG TGGACCCTCC
    ACAGCACGCA TCCGGAACAG CATAAACTTT TTGGTGGCCT ACGCATCTGG AGTGGTTAGT
    TTGCCAGATC AACAGGATTA CTGGCCGGCG ATCATAGCCA CCGATGTCCT TCTCTCCGGA
    TTTAGCAGCG AGTCCATTCT GGGTGCAGCT CTCATACCCA ATTCATCAAA TTTCCTGCAA
    CGCGACAGGA CCCTCAAGTG TGTGTTCAAA AAGGAACAAG AGGCAGCAGA AGCTTCCACC
    ACGGTTAGTG TGGATGAAGA TGATGAGGAT GAGGAAGAGG AACTGGAGTG GGAAGAGGAG
    GAGACCAAAC CCTCCAGGAT TAGCAGCTGG TGGATGCGAC TGCTCTTCGC GTTCGGTCTG
    CGTCCAGCCC CCTATGCCAA TCGACACGCT TTAAGGTTCC ACGATGATTT GGATGACCAC
    GAATTGGTCG AGGAGCGAGA GTTCACCTGG TGGACTAAGT TCTACAACTC GATGTATTGG
    AGTGCGGCCG AAATGTGCCA CGAACACAAG CACCGCCTGG TTATCTACTA CGAAGAGCTG
    GAGAAGCAGG CCCAGTTTGG ATACCTCCAG GATTGGGCGG TTCCCGTTCA GTTGGTTCAT
    GGCGTGAAGT TTAAAAAGCA TGGTCCACCC AAAGAAGATA TCTATGCCAC CTTAAAGCTG
    CAACTCAAGT TGACCCCTTG TCAGTGCCCG GTTCTTGAGG ATCCCGGAGG TGGTGGCGAC
    ATGGTTCGCA ACATGTCAAA TCCCATACAT CCTCGACATC AGTCCACACT ACAGTCGCTG
    GGTGAGACCG TAAAACTATT GGTACGTGTT TATGTAGTCC AGGGGGTTCA GATGCGTCCT
    CGAGATGTGA AAGGTGACTC CGATTGCTAT GTGAAGCTGT TCCTGGGCGG CAAGACGTTC
    TCCGATAGAG CCCATTTCTC ACCCAACCAC AGTAATCCAG TTTTCGGTCG TCTATTTGAA
    ATGGAGGCTA CGCTGCCAGG AGATCATATG CTGCAAGTCA TGGTATATGA TCATGACAAG
    ATCAAGGACG AGGTGATCGG TCAGACAAAC ATCGATCTGG AGGATCGCTG GCGAACTAGG
    CATCGTGCCA CAGTGGGTTT GGCCAATGAG TACACAAAGA GTGGCTATAA TCATTGGCAC
    GACGTTAAGT TGCCCTCGGA AATTCTCATA GATCTGTGCC AGAAAAGAGG CATTCAGGCC
    CCTTACTATT ATGGTAATGT AATTGAGGTG GACGGAATGC TTTTTGGGGA CGAAACTTTG
    ATCTCAAAAG ATGAGGAGCT GCAGGAGAGA CTTAGTTTAG CCGTGCTTAA GAATATGGAT
    AAGTTGCCAT CCTTTGGTTA CAAATTGGTT CCCGAACATG TGGAAACCCG CTCCCTTTGC
    CGCGATGATT TTCCAAATGT CGAACAGGGC AAACTACAAC TGTGGATAGA GCTGTTCGAG
    GCGAACATCT ATGTGCCCAG TCCCATCGAT ATAACCCCTG TGCCGCCCGC GGATTTCGAG
    GTGCGTGTTG TGGTTAAGAA TCTGGTCGGG ATTCAGGCGG GCGACAAAAA CATTTTTGGC
    AAACTAATGA GCGACGTTTA CGTGATAGGA TGGTGCGAGG ATGAGGACAA GCGCCAATCG
    ACCGATATCC ATTACCGCTC ATTTGCAGGA GATGCGGCAT TTAATTGGCG CATGGTCTTC
    GGCCTCAAGT ATTCCCCCAA CGAGGACCTG ATGGTAATCC GGCGAAAGGC GGGGCTGCTG
    GAGGAAATCG AGTTCAAGAA GCCGCCTATT ATCTATCTGC AGGTGTGGGA CAAGGATGTG
    TTAACCAAGG ATGAGTATCT GGCCGCACTG GAGCTCAATC TCTCCAACAT GTACGCACCG
    TATCCAGCTG CCCAGTTATG TAAGCCATAT CCCAAGAAGA GGAAGCGCAT CAATCTCTTC
    AAGCGCAAGG TCATCAACGG CTGGTTTCCG CTGCAAAGTA ATGCACATCC ATCGCAACGC
    AGCCAGGCCG CAGTTCAAAA TGGCAAAATG CAGCTGAGCA TCGAAGTCTG CACGGATGTG
    GAGGCCGTCA ACCGGCCGGC TGGTCGTGGA CAGGATCCAC CGATGGCCTT GGAGCCGCCT
    TACACATCCT CTGGCCAGTC ATTCGAAAGT ATGTCCTGTG GACTTTGCTC ATATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137916.1
    CDS1217..5413
    Translation

    Target ORF information:

    RefSeq Version NM_001144444.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster misfire (mfr), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144444.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTTTCCGT TTCTAAAGTA CAGATTCCAT CCTTTCAAGG GCAAAACGCC GGTGGTCAAG 
    AACACGACGA CGCCCGTGTG GAACTACGAG ATCAACTTTG CCTGGATGTA TCCGTCGGTG
    GCCCAGCGAT TCCTCATCCT AATTTTTGCC CATGAACATC TGCAATGGAA ATGCGTGGCT
    GAGTTTGAAT TGTGTCTTGA GGAGATCGCG TTTAATGGAA CTCCTTCTCT GGGACCCACC
    TACTTGCATC TCTACGATCC TGTCAATCCT ATGATCTATG TTGGTCGTCT TCTAATGGAA
    CTTCGGTCTG AAATGCTAAC GGATTCCCAG CCCACACACA CGCTGTCCTC CAAGACGGTG
    CCAGGTTTGG ATGAGACCCG TTTCTGGCAT GACGATGTCT ATCTGATTGA GTTTCTTCCA
    CTAATGGGTC ACCACATCCT GACCTCGGGT ACGGCATACA AGATCTCGGC GAGATTAGCC
    GAACACACCT CGAACGTGCT GGAGGGTCAA CTAAGGACAC CTTTAGATCG ATTCAAGTCG
    GCAATGAACT CAAAGAATTT CCGGCGGGAG GCACCTTTTC AGTCCTGCAT GTTTCGTGTC
    CGTTTGCCGG ACAATCGAGC CAAGTACGAA AGTGATTTCT TCATGCTGGA TATAGTCAAT
    TATATGCGTT CAGAACTGGA CACCTTCAAG CTCTTTCAAC GCAAATATCC ACTCCAGGAT
    GATGAGCAGG CCAAATGCCT GAAGGTCATT GTGAACAATA TTTTGACCAG AATCAAGGAG
    GGTCTAGATG CCCACCGTTT CGACCACGAC GTGCAACCAC AGCGCACCAC CTGGGATATC
    AATCGGCAGC TGTACCTCCT GGACTATTTC GCCAAGTTGC CACTGGAACT TCAAAATCTT
    AAACAGAAAC TACGCAGCTG CTTTCTGGAA CACCTAGACA CTTCCATTGG CGATGTGGTT
    AATGAGCTAC AGCGTTTGGT AGCTGAAATC GGATCCCTTT CCAGTATGGC ACGCACCCAG
    GACGAGTGGC CAGAATTGGT GCTTTCCTTA AGTGCAAGCG GTAAAGATGT GGGTGTTTGC
    CGATTGAACG CCAAACTCTT TTTGAATCTA CGAAATCAGG ATGCGGATCA GGATCAGGAG
    ACCCTTTGTT GGCGTCTAAA GAGCTTTTCC TTCAAGTCCG CGGGCTGTTC GCACACCTGC
    CCCAATTGTG GTTGCACCAC GGCCATAGTA TCAGGATGTT TATCCATTGT TGTGGAACGG
    GAGCGAAAAC GATTTCTTGC GGTTGTCAAT AAAGAGTGGG CCAACATTGA GCCCATGTTG
    TGGCAGCTGA ATCCTGAACA GACACACTTC CTCTGTCACG TTTACATACA CCAAGCCAAA
    GTTCGTCCTG GTGGTGAAAA AAAGAACATA TGTGACTCAC ATCTGAGAGT CCTGTTTGCC
    GACCAGGCAT GTGAAACGTA TACGTCTCCC GGTACCCTAT CGCCAATTTG GAATGCTGTG
    ATCACATTTA ACTGGGTGTC ACTACCAGGA GGCATTGGTC TCTATCTAAA GAACCCTCCT
    CTACTTTCCT TGGAATTCTA TAATTCGGAA CGCAGTCTAC CAGAAGACCT TGTGGGCATG
    GGAAGCGTGG CCATGAGTGT TATTAGCGAG GATCGGGCAA GTGATTCCAA TTGGGAGGAT
    GCAGGAGTAT TCGGTTGCTC AAAGAATCCG TTGCGAAAAC TACAGAGATT GAAGTACCTG
    ACACCACCTC CTCTCAAATG GGTGCCAATT GCTAGAAAAG GAACTCTACA AGCAGAGGTT
    TTGATGTCAG CCGAGATCAT TCAAATCTCC ATGGAATCTC TGACCACTGG TCAGAATGAT
    AAAAAGGAAC CACAACTTGC AACGGGCATT CCAATGGCCA TCAGGCCAAC TATGCAGAAT
    TTTGTCTTAG AGGTTTTCTT CGTGGGTCTA CGCAATTACT CCAAGAGCAG CATGAGTTCT
    GCTGGTAAGC GGAAAATCAA TTTAATGATG GGTGATCTGG TCCTGTCCAG TGGACCCTCC
    ACAGCACGCA TCCGGAACAG CATAAACTTT TTGGTGGCCT ACGCATCTGG AGTGGTTAGT
    TTGCCAGATC AACAGGATTA CTGGCCGGCG ATCATAGCCA CCGATGTCCT TCTCTCCGGA
    TTTAGCAGCG AGTCCATTCT GGGTGCAGCT CTCATACCCA ATTCATCAAA TTTCCTGCAA
    CGCGACAGGA CCCTCAAGTG TGTGTTCAAA AAGGAACAAG AGGCAGCAGA AGCTTCCACC
    ACGGTTAGTG TGGATGAAGA TGATGAGGAT GAGGAAGAGG AACTGGAGTG GGAAGAGGAG
    GAGACCAAAC CCTCCAGGAT TAGCAGCTGG TGGATGCGAC TGCTCTTCGC GTTCGGTCTG
    CGTCCAGCCC CCTATGCCAA TCGACACGCT TTAAGGTTCC ACGATGATTT GGATGACCAC
    GAATTGGTCG AGGAGCGAGA GTTCACCTGG TGGACTAAGT TCTACAACTC GATGTATTGG
    AGTGCGGCCG AAATGTGCCA CGAACACAAG CACCGCCTGG TTATCTACTA CGAAGAGCTG
    GAGAAGCAGG CCCAGTTTGG ATACCTCCAG GATTGGGCGG TTCCCGTTCA GTTGGTTCAT
    GGCGTGAAGT TTAAAAAGCA TGGTCCACCC AAAGAAGATA TCTATGCCAC CTTAAAGCTG
    CAACTCAAGT TGACCCCTTG TCAGTGCCCG GTTCTTGAGG ATCCCGGAGG TGGTGGCGAC
    ATGGTTCGCA ACATGTCAAA TCCCATACAT CCTCGACATC AGTCCACACT ACAGTCGCTG
    GGTGAGACCG TAAAACTATT GGTACGTGTT TATGTAGTCC AGGGGGTTCA GATGCGTCCT
    CGAGATGTGA AAGGTGACTC CGATTGCTAT GTGAAGCTGT TCCTGGGCGG CAAGACGTTC
    TCCGATAGAG CCCATTTCTC ACCCAACCAC AGTAATCCAG TTTTCGGTCG TCTATTTGAA
    ATGGAGGCTA CGCTGCCAGG AGATCATATG CTGCAAGTCA TGGTATATGA TCATGACAAG
    ATCAAGGACG AGGTGATCGG TCAGACAAAC ATCGATCTGG AGGATCGCTG GCGAACTAGG
    CATCGTGCCA CAGTGGGTTT GGCCAATGAG TACACAAAGA GTGGCTATAA TCATTGGCAC
    GACGTTAAGT TGCCCTCGGA AATTCTCATA GATCTGTGCC AGAAAAGAGG CATTCAGGCC
    CCTTACTATT ATGGTAATGT AATTGAGGTG GACGGAATGC TTTTTGGGGA CGAAACTTTG
    ATCTCAAAAG ATGAGGAGCT GCAGGAGAGA CTTAGTTTAG CCGTGCTTAA GAATATGGAT
    AAGTTGCCAT CCTTTGGTTA CAAATTGGTT CCCGAACATG TGGAAACCCG CTCCCTTTGC
    CGCGATGATT TTCCAAATGT CGAACAGGGC AAACTACAAC TGTGGATAGA GCTGTTCGAG
    GCGAACATCT ATGTGCCCAG TCCCATCGAT ATAACCCCTG TGCCGCCCGC GGATTTCGAG
    GTGCGTGTTG TGGTTAAGAA TCTGGTCGGG ATTCAGGCGG GCGACAAAAA CATTTTTGGC
    AAACTAATGA GCGACGTTTA CGTGATAGGA TGGTGCGAGG ATGAGGACAA GCGCCAATCG
    ACCGATATCC ATTACCGCTC ATTTGCAGGA GATGCGGCAT TTAATTGGCG CATGGTCTTC
    GGCCTCAAGT ATTCCCCCAA CGAGGACCTG ATGGTAATCC GGCGAAAGGC GGGGCTGCTG
    GAGGAAATCG AGTTCAAGAA GCCGCCTATT ATCTATCTGC AGGTGTGGGA CAAGGATGTG
    TTAACCAAGG ATGAGTATCT GGCCGCACTG GAGCTCAATC TCTCCAACAT GTACGCACCG
    TATCCAGCTG CCCAGTTATG TAAGCCATAT CCCAAGAAGA GGAAGCGCAT CAATCTCTTC
    AAGCGCAAGG TCATCAACGG CTGGTTTCCG CTGCAAAGTA ATGCACATCC ATCGCAACGC
    AGCCAGGCCG CAGTTCAAAA TGGCAAAATG CAGCTGAGCA TCGAAGTCTG CACGGATGTG
    GAGGCCGTCA ACCGGCCGGC TGGTCGTGGA CAGGATCCAC CGATGGCCTT GGAGCCGCCT
    TACACATCCT CTGGCCAGTC ATTCGAAAGT ATGTCCTGTG GACTTTGCTC ATATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa17952
    Clone ID Related Accession (Same CDS sequence) NM_001144447.2
    Accession Version NM_001144447.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4944bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product misfire, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001144447.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAGTTACA TGGACGACTG TTTCGCAGGT GCTCCGCAGG AGTTTCTCAT CTGCATCACG 
    GTGCACAAGG CTGCCCAAGT GGGAGTGACC ACCGGCGAGC TGTACGTGCG GATTAGCTTG
    GACAAAATGA CGAAGAGCAC CAAATCCTTT CCTAATACAG AAAATCCCTT CTTCAATGAG
    TACTTCGTTT TTGAGTTCCA TTGTACGTTA ACCGAACTCC TGCGCTTAAC GATCCTTTTT
    GAGCTAAAGA AACACATGAC GTATAAGAAA AATGTGGTAG TGGGTGAACT ACTGGTTGAT
    CTACATAGTG TGTGGAACCA GTCCAATCAT GGCTACTTCA AGAAGTGGGG TCGCCTGGAA
    GCTCCTATTG GAGAAAACCA GACCCTAGAG AATGCCGTTG CACCGCACGG CTATTTGCAA
    CTGGATCTGG CCATCGTATC CCAGCACAGT CCGGTGTCTT ATGCCCTCGG ATCTGAGGAG
    CAGGACACAC AAAATCTGAA CAAGTGGACG GTTGATCATG ATTTTGATGA CATCGAAAAG
    AATCTACTGA CCAACGTGAA CATCTTCGCC CCAAGTAATA TTCGATACTT CGTCGCCTTT
    TACCGGGGTT ACTTCATCAA GCAGAGCAAC TACATGATTC AAGTATCGTT TGCCGGCTTC
    AATGGCAAAA CGCCGGTGGT CAAGAACACG ACGACGCCCG TGTGGAACTA CGAGATCAAC
    TTTGCCTGGA TGTATCCGTC GGTGGCCCAG CGATTCCTCA TCCTAATTTT TGCCCATGAA
    CATCTGCAAT GGAAATGCGT GGCTGAGTTT GAATTGTGTC TTGAGGAGAT CGCGTTTAAT
    GGAACTCCTT CTCTGGGACC CACCTACTTG CATCTCTACG ATCCTGTCAA TCCTATGATC
    TATGTTGGTC GTCTTCTAAT GGAACTTCGG TCTGAAATGC TAACGGATTC CCAGCCCACA
    CACACGCTGT CCTCCAAGAC GGTGCCAGGT TTGGATGAGA CCCGTTTCTG GCATGACGAT
    GTCTATCTGA TTGAGTTTCT TCCACTAATG GGTCACCACA TCCTGACCTC GGGTACGGCA
    TACAAGATCT CGGCGAGATT AGCCGAACAC ACCTCGAACG TGCTGGAGGG TCAACTAAGG
    ACACCTTTAG ATCGATTCAA GTCGGCAATG AACTCAAAGA ATTTCCGGCG GGAGGCACCT
    TTTCAGTCCT GCATGTTTCG TGTCCGTTTG CCGGACAATC GAGCCAAGTA CGAAAGTGAT
    TTCTTCATGC TGGATATAGT CAATTATATG CGTTCAGAAC TGGACACCTT CAAGCTCTTT
    CAACGCAAAT ATCCACTCCA GGATGATGAG CAGGCCAAAT GCCTGAAGGT CATTGTGAAC
    AATATTTTGA CCAGAATCAA GGAGGGTCTA GATGCCCACC GTTTCGACCA CGACGTGCAA
    CCACAGCGCA CCACCTGGGA TATCAATCGG CAGCTGTACC TCCTGGACTA TTTCGCCAAG
    TTGCCACTGG AACTTCAAAA TCTTAAACAG AAACTACGCA GCTGCTTTCT GGAACACCTA
    GACACTTCCA TTGGCGATGT GGTTAATGAG CTACAGCGTT TGGTAGCTGA AATCGGATCC
    CTTTCCAGTA TGGCACGCAC CCAGGACGAG TGGCCAGAAT TGGTGCTTTC CTTAAGTGCA
    AGCGGTAAAG ATGTGGGTGT TTGCCGATTG AACGCCAAAC TCTTTTTGAA TCTACGAAAT
    CAGGATGCGG ATCAGGATCA GGAGACCCTT TGTTGGCGTC TAAAGAGCTT TTCCTTCAAG
    TCCGCGGGCT GTTCGCACAC CTGCCCCAAT TGTGGTTGCA CCACGGCCAT AGTATCAGGA
    TGTTTATCCA TTGTTGTGGA ACGGGAGCGA AAACGATTTC TTGCGGTTGT CAATAAAGAG
    TGGGCCAACA TTGAGCCCAT GTTGTGGCAG CTGAATCCTG AACAGACACA CTTCCTCTGT
    CACGTTTACA TACACCAAGC CAAAGTTCGT CCTGGTGGTG AAAAAAAGAA CATATGTGAC
    TCACATCTGA GAGTCCTGTT TGCCGACCAG GCATGTGAAA CGTATACGTC TCCCGGTACC
    CTATCGCCAA TTTGGAATGC TGTGATCACA TTTAACTGGG TGTCACTACC AGGAGGCATT
    GGTCTCTATC TAAAGAACCC TCCTCTACTT TCCTTGGAAT TCTATAATTC GGAACGCAGT
    CTACCAGAAG ACCTTGTGGG CATGGGAAGC GTGGCCATGA GTGTTATTAG CGAGGATCGG
    GCAAGTGATT CCAATTGGGA GGATGCAGGA GTATTCGGTT GCTCAAAGAA TCCGTTGCGA
    AAACTACAGA GATTGAAGTA CCTGACACCA CCTCCTCTCA AATGGGTGCC AATTGCTAGA
    AAAGGAACTC TACAAGCAGA GGTTTTGATG TCAGCCGAGA TCATTCAAAT CTCCATGGAA
    TCTCTGACCA CTGGTCAGAA TGATAAAAAG GAACCACAAC TTGCAACGGG CATTCCAATG
    GCCATCAGGC CAACTATGCA GAATTTTGTC TTAGAGGTTT TCTTCGTGGG TCTACGCAAT
    TACTCCAAGA GCAGCATGAG TTCTGCTGGT AAGCGGAAAA TCAATTTAAT GATGGGTGAT
    CTGGTCCTGT CCAGTGGACC CTCCACAGCA CGCATCCGGA ACAGCATAAA CTTTTTGGTG
    GCCTACGCAT CTGGAGTGGT TAGTTTGCCA GATCAACAGG ATTACTGGCC GGCGATCATA
    GCCACCGATG TCCTTCTCTC CGGATTTAGC AGCGAGTCCA TTCTGGGTGC AGCTCTCATA
    CCCAATTCAT CAAATTTCCT GCAACGCGAC AGGACCCTCA AGTGTGTGTT CAAAAAGGAA
    CAAGAGGCAG CAGAAGCTTC CACCACGGTT AGTGTGGATG AAGATGATGA GGATGAGGAA
    GAGGAACTGG AGTGGGAAGA GGAGGAGACC AAACCCTCCA GGATTAGCAG CTGGTGGATG
    CGACTGCTCT TCGCGTTCGG TCTGCGTCCA GCCCCCTATG CCAATCGACA CGCTTTAAGG
    TTCCACGATG ATTTGGATGA CCACGAATTG GTCGAGGAGC GAGAGTTCAC CTGGTGGACT
    AAGTTCTACA ACTCGATGTA TTGGAGTGCG GCCGAAATGT GCCACGAACA CAAGCACCGC
    CTGGTTATCT ACTACGAAGA GCTGGAGAAG CAGGCCCAGT TTGGATACCT CCAGGATTGG
    GCGGTTCCCG TTCAGTTGGT TCATGGCGTG AAGTTTAAAA AGCATGGTCC ACCCAAAGAA
    GATATCTATG CCACCTTAAA GCTGCAACTC AAGTTGACCC CTTGTCAGTG CCCGGTTCTT
    GAGGATCCCG GAGGTGGTGG CGACATGGTT CGCAACATGT CAAATCCCAT ACATCCTCGA
    CATCAGTCCA CACTACAGTC GCTGGGTGAG ACCGTAAAAC TATTGGTACG TGTTTATGTA
    GTCCAGGGGG TTCAGATGCG TCCTCGAGAT GTGAAAGGTG ACTCCGATTG CTATGTGAAG
    CTGTTCCTGG GCGGCAAGAC GTTCTCCGAT AGAGCCCATT TCTCACCCAA CCACAGTAAT
    CCAGTTTTCG GTCGTCTATT TGAAATGGAG GCTACGCTGC CAGGAGATCA TATGCTGCAA
    GTCATGGTAT ATGATCATGA CAAGATCAAG GACGAGGTGA TCGGTCAGAC AAACATCGAT
    CTGGAGGATC GCTGGCGAAC TAGGCATCGT GCCACAGTGG GTTTGGCCAA TGAGTACACA
    AAGAGTGGCT ATAATCATTG GCACGACGTT AAGTTGCCCT CGGAAATTCT CATAGATCTG
    TGCCAGAAAA GAGGCATTCA GGCCCCTTAC TATTATGGTA ATGTAATTGA GGTGGACGGA
    ATGCTTTTTG GGGACGAAAC TTTGATCTCA AAAGATGAGG AGCTGCAGGA GAGACTTAGT
    TTAGCCGTGC TTAAGAATAT GGATAAGTTG CCATCCTTTG GTTACAAATT GGTTCCCGAA
    CATGTGGAAA CCCGCTCCCT TTGCCGCGAT GATTTTCCAA ATGTCGAACA GGGCAAACTA
    CAACTGTGGA TAGAGCTGTT CGAGGCGAAC ATCTATGTGC CCAGTCCCAT CGATATAACC
    CCTGTGCCGC CCGCGGATTT CGAGGTGCGT GTTGTGGTTA AGAATCTGGT CGGGATTCAG
    GCGGGCGACA AAAACATTTT TGGCAAACTA ATGAGCGACG TTTACGTGAT AGGATGGTGC
    GAGGATGAGG ACAAGCGCCA ATCGACCGAT ATCCATTACC GCTCATTTGC AGGAGATGCG
    GCATTTAATT GGCGCATGGT CTTCGGCCTC AAGTATTCCC CCAACGAGGA CCTGATGGTA
    ATCCGGCGAA AGGCGGGGCT GCTGGAGGAA ATCGAGTTCA AGAAGCCGCC TATTATCTAT
    CTGCAGGTGT GGGACAAGGA TGTGTTAACC AAGGATGAGT ATCTGGCCGC ACTGGAGCTC
    AATCTCTCCA ACATGTACGC ACCGTATCCA GCTGCCCAGT TATGTAAGCC ATATCCCAAG
    AAGAGGAAGC GCATCAATCT CTTCAAGCGC AAGGTCATCA ACGGCTGGTT TCCGCTGCAA
    AGTAATGCAC ATCCATCGCA ACGCAGCCAG GCCGCAGTTC AAAATGGCAA AATGCAGCTG
    AGCATCGAAG TCTGCACGGA TGTGGAGGCC GTCAACCGGC CGGCTGGTCG TGGACAGGAT
    CCACCGATGG CCTTGGAGCC GCCTTACAGA CCGGACAGCT CGTTCAATCC ACTAACGCAT
    CCTTGCAAAT CCTTTCAGCA CATCCTCTGG CCAGTCATTC GAAAGTATGT CCTGTGGACT
    TTGCTCATAT TGATCGTTAT CCTGGGATTG GTGCTCTTCT TTTCCAATCT GCCCGCCAAA
    CTCATGACCA TTCCACTTGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137919.1
    CDS130..5073
    Translation

    Target ORF information:

    RefSeq Version NM_001144447.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster misfire (mfr), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144447.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAGTTACA TGGACGACTG TTTCGCAGGT GCTCCGCAGG AGTTTCTCAT CTGCATCACG 
    GTGCACAAGG CTGCCCAAGT GGGAGTGACC ACCGGCGAGC TGTACGTGCG GATTAGCTTG
    GACAAAATGA CGAAGAGCAC CAAATCCTTT CCTAATACAG AAAATCCCTT CTTCAATGAG
    TACTTCGTTT TTGAGTTCCA TTGTACGTTA ACCGAACTCC TGCGCTTAAC GATCCTTTTT
    GAGCTAAAGA AACACATGAC GTATAAGAAA AATGTGGTAG TGGGTGAACT ACTGGTTGAT
    CTACATAGTG TGTGGAACCA GTCCAATCAT GGCTACTTCA AGAAGTGGGG TCGCCTGGAA
    GCTCCTATTG GAGAAAACCA GACCCTAGAG AATGCCGTTG CACCGCACGG CTATTTGCAA
    CTGGATCTGG CCATCGTATC CCAGCACAGT CCGGTGTCTT ATGCCCTCGG ATCTGAGGAG
    CAGGACACAC AAAATCTGAA CAAGTGGACG GTTGATCATG ATTTTGATGA CATCGAAAAG
    AATCTACTGA CCAACGTGAA CATCTTCGCC CCAAGTAATA TTCGATACTT CGTCGCCTTT
    TACCGGGGTT ACTTCATCAA GCAGAGCAAC TACATGATTC AAGTATCGTT TGCCGGCTTC
    AATGGCAAAA CGCCGGTGGT CAAGAACACG ACGACGCCCG TGTGGAACTA CGAGATCAAC
    TTTGCCTGGA TGTATCCGTC GGTGGCCCAG CGATTCCTCA TCCTAATTTT TGCCCATGAA
    CATCTGCAAT GGAAATGCGT GGCTGAGTTT GAATTGTGTC TTGAGGAGAT CGCGTTTAAT
    GGAACTCCTT CTCTGGGACC CACCTACTTG CATCTCTACG ATCCTGTCAA TCCTATGATC
    TATGTTGGTC GTCTTCTAAT GGAACTTCGG TCTGAAATGC TAACGGATTC CCAGCCCACA
    CACACGCTGT CCTCCAAGAC GGTGCCAGGT TTGGATGAGA CCCGTTTCTG GCATGACGAT
    GTCTATCTGA TTGAGTTTCT TCCACTAATG GGTCACCACA TCCTGACCTC GGGTACGGCA
    TACAAGATCT CGGCGAGATT AGCCGAACAC ACCTCGAACG TGCTGGAGGG TCAACTAAGG
    ACACCTTTAG ATCGATTCAA GTCGGCAATG AACTCAAAGA ATTTCCGGCG GGAGGCACCT
    TTTCAGTCCT GCATGTTTCG TGTCCGTTTG CCGGACAATC GAGCCAAGTA CGAAAGTGAT
    TTCTTCATGC TGGATATAGT CAATTATATG CGTTCAGAAC TGGACACCTT CAAGCTCTTT
    CAACGCAAAT ATCCACTCCA GGATGATGAG CAGGCCAAAT GCCTGAAGGT CATTGTGAAC
    AATATTTTGA CCAGAATCAA GGAGGGTCTA GATGCCCACC GTTTCGACCA CGACGTGCAA
    CCACAGCGCA CCACCTGGGA TATCAATCGG CAGCTGTACC TCCTGGACTA TTTCGCCAAG
    TTGCCACTGG AACTTCAAAA TCTTAAACAG AAACTACGCA GCTGCTTTCT GGAACACCTA
    GACACTTCCA TTGGCGATGT GGTTAATGAG CTACAGCGTT TGGTAGCTGA AATCGGATCC
    CTTTCCAGTA TGGCACGCAC CCAGGACGAG TGGCCAGAAT TGGTGCTTTC CTTAAGTGCA
    AGCGGTAAAG ATGTGGGTGT TTGCCGATTG AACGCCAAAC TCTTTTTGAA TCTACGAAAT
    CAGGATGCGG ATCAGGATCA GGAGACCCTT TGTTGGCGTC TAAAGAGCTT TTCCTTCAAG
    TCCGCGGGCT GTTCGCACAC CTGCCCCAAT TGTGGTTGCA CCACGGCCAT AGTATCAGGA
    TGTTTATCCA TTGTTGTGGA ACGGGAGCGA AAACGATTTC TTGCGGTTGT CAATAAAGAG
    TGGGCCAACA TTGAGCCCAT GTTGTGGCAG CTGAATCCTG AACAGACACA CTTCCTCTGT
    CACGTTTACA TACACCAAGC CAAAGTTCGT CCTGGTGGTG AAAAAAAGAA CATATGTGAC
    TCACATCTGA GAGTCCTGTT TGCCGACCAG GCATGTGAAA CGTATACGTC TCCCGGTACC
    CTATCGCCAA TTTGGAATGC TGTGATCACA TTTAACTGGG TGTCACTACC AGGAGGCATT
    GGTCTCTATC TAAAGAACCC TCCTCTACTT TCCTTGGAAT TCTATAATTC GGAACGCAGT
    CTACCAGAAG ACCTTGTGGG CATGGGAAGC GTGGCCATGA GTGTTATTAG CGAGGATCGG
    GCAAGTGATT CCAATTGGGA GGATGCAGGA GTATTCGGTT GCTCAAAGAA TCCGTTGCGA
    AAACTACAGA GATTGAAGTA CCTGACACCA CCTCCTCTCA AATGGGTGCC AATTGCTAGA
    AAAGGAACTC TACAAGCAGA GGTTTTGATG TCAGCCGAGA TCATTCAAAT CTCCATGGAA
    TCTCTGACCA CTGGTCAGAA TGATAAAAAG GAACCACAAC TTGCAACGGG CATTCCAATG
    GCCATCAGGC CAACTATGCA GAATTTTGTC TTAGAGGTTT TCTTCGTGGG TCTACGCAAT
    TACTCCAAGA GCAGCATGAG TTCTGCTGGT AAGCGGAAAA TCAATTTAAT GATGGGTGAT
    CTGGTCCTGT CCAGTGGACC CTCCACAGCA CGCATCCGGA ACAGCATAAA CTTTTTGGTG
    GCCTACGCAT CTGGAGTGGT TAGTTTGCCA GATCAACAGG ATTACTGGCC GGCGATCATA
    GCCACCGATG TCCTTCTCTC CGGATTTAGC AGCGAGTCCA TTCTGGGTGC AGCTCTCATA
    CCCAATTCAT CAAATTTCCT GCAACGCGAC AGGACCCTCA AGTGTGTGTT CAAAAAGGAA
    CAAGAGGCAG CAGAAGCTTC CACCACGGTT AGTGTGGATG AAGATGATGA GGATGAGGAA
    GAGGAACTGG AGTGGGAAGA GGAGGAGACC AAACCCTCCA GGATTAGCAG CTGGTGGATG
    CGACTGCTCT TCGCGTTCGG TCTGCGTCCA GCCCCCTATG CCAATCGACA CGCTTTAAGG
    TTCCACGATG ATTTGGATGA CCACGAATTG GTCGAGGAGC GAGAGTTCAC CTGGTGGACT
    AAGTTCTACA ACTCGATGTA TTGGAGTGCG GCCGAAATGT GCCACGAACA CAAGCACCGC
    CTGGTTATCT ACTACGAAGA GCTGGAGAAG CAGGCCCAGT TTGGATACCT CCAGGATTGG
    GCGGTTCCCG TTCAGTTGGT TCATGGCGTG AAGTTTAAAA AGCATGGTCC ACCCAAAGAA
    GATATCTATG CCACCTTAAA GCTGCAACTC AAGTTGACCC CTTGTCAGTG CCCGGTTCTT
    GAGGATCCCG GAGGTGGTGG CGACATGGTT CGCAACATGT CAAATCCCAT ACATCCTCGA
    CATCAGTCCA CACTACAGTC GCTGGGTGAG ACCGTAAAAC TATTGGTACG TGTTTATGTA
    GTCCAGGGGG TTCAGATGCG TCCTCGAGAT GTGAAAGGTG ACTCCGATTG CTATGTGAAG
    CTGTTCCTGG GCGGCAAGAC GTTCTCCGAT AGAGCCCATT TCTCACCCAA CCACAGTAAT
    CCAGTTTTCG GTCGTCTATT TGAAATGGAG GCTACGCTGC CAGGAGATCA TATGCTGCAA
    GTCATGGTAT ATGATCATGA CAAGATCAAG GACGAGGTGA TCGGTCAGAC AAACATCGAT
    CTGGAGGATC GCTGGCGAAC TAGGCATCGT GCCACAGTGG GTTTGGCCAA TGAGTACACA
    AAGAGTGGCT ATAATCATTG GCACGACGTT AAGTTGCCCT CGGAAATTCT CATAGATCTG
    TGCCAGAAAA GAGGCATTCA GGCCCCTTAC TATTATGGTA ATGTAATTGA GGTGGACGGA
    ATGCTTTTTG GGGACGAAAC TTTGATCTCA AAAGATGAGG AGCTGCAGGA GAGACTTAGT
    TTAGCCGTGC TTAAGAATAT GGATAAGTTG CCATCCTTTG GTTACAAATT GGTTCCCGAA
    CATGTGGAAA CCCGCTCCCT TTGCCGCGAT GATTTTCCAA ATGTCGAACA GGGCAAACTA
    CAACTGTGGA TAGAGCTGTT CGAGGCGAAC ATCTATGTGC CCAGTCCCAT CGATATAACC
    CCTGTGCCGC CCGCGGATTT CGAGGTGCGT GTTGTGGTTA AGAATCTGGT CGGGATTCAG
    GCGGGCGACA AAAACATTTT TGGCAAACTA ATGAGCGACG TTTACGTGAT AGGATGGTGC
    GAGGATGAGG ACAAGCGCCA ATCGACCGAT ATCCATTACC GCTCATTTGC AGGAGATGCG
    GCATTTAATT GGCGCATGGT CTTCGGCCTC AAGTATTCCC CCAACGAGGA CCTGATGGTA
    ATCCGGCGAA AGGCGGGGCT GCTGGAGGAA ATCGAGTTCA AGAAGCCGCC TATTATCTAT
    CTGCAGGTGT GGGACAAGGA TGTGTTAACC AAGGATGAGT ATCTGGCCGC ACTGGAGCTC
    AATCTCTCCA ACATGTACGC ACCGTATCCA GCTGCCCAGT TATGTAAGCC ATATCCCAAG
    AAGAGGAAGC GCATCAATCT CTTCAAGCGC AAGGTCATCA ACGGCTGGTT TCCGCTGCAA
    AGTAATGCAC ATCCATCGCA ACGCAGCCAG GCCGCAGTTC AAAATGGCAA AATGCAGCTG
    AGCATCGAAG TCTGCACGGA TGTGGAGGCC GTCAACCGGC CGGCTGGTCG TGGACAGGAT
    CCACCGATGG CCTTGGAGCC GCCTTACAGA CCGGACAGCT CGTTCAATCC ACTAACGCAT
    CCTTGCAAAT CCTTTCAGCA CATCCTCTGG CCAGTCATTC GAAAGTATGT CCTGTGGACT
    TTGCTCATAT TGATCGTTAT CCTGGGATTG GTGCTCTTCT TTTCCAATCT GCCCGCCAAA
    CTCATGACCA TTCCACTTGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa17954
    Clone ID Related Accession (Same CDS sequence) NM_001144445.2
    Accession Version NM_001144445.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4056bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product misfire, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001144445.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAACATCT GCAATGGAAA TGCGTGGCTG AGTTTGAATT GTGTCTTGAG GAGATCGCGT 
    TTAATGCCCA CACACACGCT GTCCTCCAAG ACGGTGCCAG GTTTGGATGA GACCCGTTTC
    TGGCATGACG ATGTCTATCT GATTGAGTTT CTTCCACTAA TGGGTCACCA CATCCTGACC
    TCGGGTACGG CATACAAGAT CTCGGCGAGA TTAGCCGAAC ACACCTCGAA CGTGCTGGAG
    GGTCAACTAA GGACACCTTT AGATCGATTC AAGTCGGCAA TGAACTCAAA GAATTTCCGG
    CGGGAGGCAC CTTTTCAGTC CTGCATGTTT CGTGTCCGTT TGCCGGACAA TCGAGCCAAG
    TACGAAAGTG ATTTCTTCAT GCTGGATATA GTCAATTATA TGCGTTCAGA ACTGGACACC
    TTCAAGCTCT TTCAACGCAA ATATCCACTC CAGGATGATG AGCAGGCCAA ATGCCTGAAG
    GTCATTGTGA ACAATATTTT GACCAGAATC AAGGAGGGTC TAGATGCCCA CCGTTTCGAC
    CACGACGTGC AACCACAGCG CACCACCTGG GATATCAATC GGCAGCTGTA CCTCCTGGAC
    TATTTCGCCA AGTTGCCACT GGAACTTCAA AATCTTAAAC AGAAACTACG CAGCTGCTTT
    CTGGAACACC TAGACACTTC CATTGGCGAT GTGGTTAATG AGCTACAGCG TTTGGTAGCT
    GAAATCGGAT CCCTTTCCAG TATGGCACGC ACCCAGGACG AGTGGCCAGA ATTGGTGCTT
    TCCTTAAGTG CAAGCGGTAA AGATGTGGGT GTTTGCCGAT TGAACGCCAA ACTCTTTTTG
    AATCTACGAA ATCAGGATGC GGATCAGGAT CAGGAGACCC TTTGTTGGCG TCTAAAGAGC
    TTTTCCTTCA AGTCCGCGGG CTGTTCGCAC ACCTGCCCCA ATTGTGGTTG CACCACGGCC
    ATAGTATCAG GATGTTTATC CATTGTTGTG GAACGGGAGC GAAAACGATT TCTTGCGGTT
    GTCAATAAAG AGTGGGCCAA CATTGAGCCC ATGTTGTGGC AGCTGAATCC TGAACAGACA
    CACTTCCTCT GTCACGTTTA CATACACCAA GCCAAAGTTC GTCCTGGTGG TGAAAAAAAG
    AACATATGTG ACTCACATCT GAGAGTCCTG TTTGCCGACC AGGCATGTGA AACGTATACG
    TCTCCCGGTA CCCTATCGCC AATTTGGAAT GCTGTGATCA CATTTAACTG GGTGTCACTA
    CCAGGAGGCA TTGGTCTCTA TCTAAAGAAC CCTCCTCTAC TTTCCTTGGA ATTCTATAAT
    TCGGAACGCA GTCTACCAGA AGACCTTGTG GGCATGGGAA GCGTGGCCAT GAGTGTTATT
    AGCGAGGATC GGGCAAGTGA TTCCAATTGG GAGGATGCAG GAGTATTCGG TTGCTCAAAG
    AATCCGTTGC GAAAACTACA GAGATTGAAG TACCTGACAC CACCTCCTCT CAAATGGGTG
    CCAATTGCTA GAAAAGGAAC TCTACAAGCA GAGGTTTTGA TGTCAGCCGA GATCATTCAA
    ATCTCCATGG AATCTCTGAC CACTGGTCAG AATGATAAAA AGGAACCACA ACTTGCAACG
    GGCATTCCAA TGGCCATCAG GCCAACTATG CAGAATTTTG TCTTAGAGGT TTTCTTCGTG
    GGTCTACGCA ATTACTCCAA GAGCAGCATG AGTTCTGCTG GTAAGCGGAA AATCAATTTA
    ATGATGGGTG ATCTGGTCCT GTCCAGTGGA CCCTCCACAG CACGCATCCG GAACAGCATA
    AACTTTTTGG TGGCCTACGC ATCTGGAGTG GTTAGTTTGC CAGATCAACA GGATTACTGG
    CCGGCGATCA TAGCCACCGA TGTCCTTCTC TCCGGATTTA GCAGCGAGTC CATTCTGGGT
    GCAGCTCTCA TACCCAATTC ATCAAATTTC CTGCAACGCG ACAGGACCCT CAAGTGTGTG
    TTCAAAAAGG AACAAGAGGC AGCAGAAGCT TCCACCACGG TTAGTGTGGA TGAAGATGAT
    GAGGATGAGG AAGAGGAACT GGAGTGGGAA GAGGAGGAGA CCAAACCCTC CAGGATTAGC
    AGCTGGTGGA TGCGACTGCT CTTCGCGTTC GGTCTGCGTC CAGCCCCCTA TGCCAATCGA
    CACGCTTTAA GGTTCCACGA TGATTTGGAT GACCACGAAT TGGTCGAGGA GCGAGAGTTC
    ACCTGGTGGA CTAAGTTCTA CAACTCGATG TATTGGAGTG CGGCCGAAAT GTGCCACGAA
    CACAAGCACC GCCTGGTTAT CTACTACGAA GAGCTGGAGA AGCAGGCCCA GTTTGGATAC
    CTCCAGGATT GGGCGGTTCC CGTTCAGTTG GTTCATGGCG TGAAGTTTAA AAAGCATGGT
    CCACCCAAAG AAGATATCTA TGCCACCTTA AAGCTGCAAC TCAAGTTGAC CCCTTGTCAG
    TGCCCGGTTC TTGAGGATCC CGGAGGTGGT GGCGACATGG TTCGCAACAT GTCAAATCCC
    ATACATCCTC GACATCAGTC CACACTACAG TCGCTGGGTG AGACCGTAAA ACTATTGGTA
    CGTGTTTATG TAGTCCAGGG GGTTCAGATG CGTCCTCGAG ATGTGAAAGG TGACTCCGAT
    TGCTATGTGA AGCTGTTCCT GGGCGGCAAG ACGTTCTCCG ATAGAGCCCA TTTCTCACCC
    AACCACAGTA ATCCAGTTTT CGGTCGTCTA TTTGAAATGG AGGCTACGCT GCCAGGAGAT
    CATATGCTGC AAGTCATGGT ATATGATCAT GACAAGATCA AGGACGAGGT GATCGGTCAG
    ACAAACATCG ATCTGGAGGA TCGCTGGCGA ACTAGGCATC GTGCCACAGT GGGTTTGGCC
    AATGAGTACA CAAAGAGTGG CTATAATCAT TGGCACGACG TTAAGTTGCC CTCGGAAATT
    CTCATAGATC TGTGCCAGAA AAGAGGCATT CAGGCCCCTT ACTATTATGG TAATGTAATT
    GAGGTGGACG GAATGCTTTT TGGGGACGAA ACTTTGATCT CAAAAGATGA GGAGCTGCAG
    GAGAGACTTA GTTTAGCCGT GCTTAAGAAT ATGGATAAGT TGCCATCCTT TGGTTACAAA
    TTGGTTCCCG AACATGTGGA AACCCGCTCC CTTTGCCGCG ATGATTTTCC AAATGTCGAA
    CAGGGCAAAC TACAACTGTG GATAGAGCTG TTCGAGGCGA ACATCTATGT GCCCAGTCCC
    ATCGATATAA CCCCTGTGCC GCCCGCGGAT TTCGAGGTGC GTGTTGTGGT TAAGAATCTG
    GTCGGGATTC AGGCGGGCGA CAAAAACATT TTTGGCAAAC TAATGAGCGA CGTTTACGTG
    ATAGGATGGT GCGAGGATGA GGACAAGCGC CAATCGACCG ATATCCATTA CCGCTCATTT
    GCAGGAGATG CGGCATTTAA TTGGCGCATG GTCTTCGGCC TCAAGTATTC CCCCAACGAG
    GACCTGATGG TAATCCGGCG AAAGGCGGGG CTGCTGGAGG AAATCGAGTT CAAGAAGCCG
    CCTATTATCT ATCTGCAGGT GTGGGACAAG GATGTGTTAA CCAAGGATGA GTATCTGGCC
    GCACTGGAGC TCAATCTCTC CAACATGTAC GCACCGTATC CAGCTGCCCA GTTATGTAAG
    CCATATCCCA AGAAGAGGAA GCGCATCAAT CTCTTCAAGC GCAAGGTCAT CAACGGCTGG
    TTTCCGCTGC AAAGTAATGC ACATCCATCG CAACGCAGCC AGGCCGCAGT TCAAAATGGC
    AAAATGCAGC TGAGCATCGA AGTCTGCACG GATGTGGAGG CCGTCAACCG GCCGGCTGGT
    CGTGGACAGG ATCCACCGAT GGCCTTGGAG CCGCCTTACA GACCGGACAG CTCGTTCAAT
    CCACTAACGC ATCCTTGCAA ATCCTTTCAG CACATCCTCT GGCCAGTCAT TCGAAAGTAT
    GTCCTGTGGA CTTTGCTCAT ATTGATCGTT ATCCTGGGAT TGGTGCTCTT CTTTTCCAAT
    CTGCCCGCCA AACTCATGAC CATTCCACTT GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137917.1
    CDS951..5006
    Translation

    Target ORF information:

    RefSeq Version NM_001144445.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster misfire (mfr), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144445.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAACATCT GCAATGGAAA TGCGTGGCTG AGTTTGAATT GTGTCTTGAG GAGATCGCGT 
    TTAATGCCCA CACACACGCT GTCCTCCAAG ACGGTGCCAG GTTTGGATGA GACCCGTTTC
    TGGCATGACG ATGTCTATCT GATTGAGTTT CTTCCACTAA TGGGTCACCA CATCCTGACC
    TCGGGTACGG CATACAAGAT CTCGGCGAGA TTAGCCGAAC ACACCTCGAA CGTGCTGGAG
    GGTCAACTAA GGACACCTTT AGATCGATTC AAGTCGGCAA TGAACTCAAA GAATTTCCGG
    CGGGAGGCAC CTTTTCAGTC CTGCATGTTT CGTGTCCGTT TGCCGGACAA TCGAGCCAAG
    TACGAAAGTG ATTTCTTCAT GCTGGATATA GTCAATTATA TGCGTTCAGA ACTGGACACC
    TTCAAGCTCT TTCAACGCAA ATATCCACTC CAGGATGATG AGCAGGCCAA ATGCCTGAAG
    GTCATTGTGA ACAATATTTT GACCAGAATC AAGGAGGGTC TAGATGCCCA CCGTTTCGAC
    CACGACGTGC AACCACAGCG CACCACCTGG GATATCAATC GGCAGCTGTA CCTCCTGGAC
    TATTTCGCCA AGTTGCCACT GGAACTTCAA AATCTTAAAC AGAAACTACG CAGCTGCTTT
    CTGGAACACC TAGACACTTC CATTGGCGAT GTGGTTAATG AGCTACAGCG TTTGGTAGCT
    GAAATCGGAT CCCTTTCCAG TATGGCACGC ACCCAGGACG AGTGGCCAGA ATTGGTGCTT
    TCCTTAAGTG CAAGCGGTAA AGATGTGGGT GTTTGCCGAT TGAACGCCAA ACTCTTTTTG
    AATCTACGAA ATCAGGATGC GGATCAGGAT CAGGAGACCC TTTGTTGGCG TCTAAAGAGC
    TTTTCCTTCA AGTCCGCGGG CTGTTCGCAC ACCTGCCCCA ATTGTGGTTG CACCACGGCC
    ATAGTATCAG GATGTTTATC CATTGTTGTG GAACGGGAGC GAAAACGATT TCTTGCGGTT
    GTCAATAAAG AGTGGGCCAA CATTGAGCCC ATGTTGTGGC AGCTGAATCC TGAACAGACA
    CACTTCCTCT GTCACGTTTA CATACACCAA GCCAAAGTTC GTCCTGGTGG TGAAAAAAAG
    AACATATGTG ACTCACATCT GAGAGTCCTG TTTGCCGACC AGGCATGTGA AACGTATACG
    TCTCCCGGTA CCCTATCGCC AATTTGGAAT GCTGTGATCA CATTTAACTG GGTGTCACTA
    CCAGGAGGCA TTGGTCTCTA TCTAAAGAAC CCTCCTCTAC TTTCCTTGGA ATTCTATAAT
    TCGGAACGCA GTCTACCAGA AGACCTTGTG GGCATGGGAA GCGTGGCCAT GAGTGTTATT
    AGCGAGGATC GGGCAAGTGA TTCCAATTGG GAGGATGCAG GAGTATTCGG TTGCTCAAAG
    AATCCGTTGC GAAAACTACA GAGATTGAAG TACCTGACAC CACCTCCTCT CAAATGGGTG
    CCAATTGCTA GAAAAGGAAC TCTACAAGCA GAGGTTTTGA TGTCAGCCGA GATCATTCAA
    ATCTCCATGG AATCTCTGAC CACTGGTCAG AATGATAAAA AGGAACCACA ACTTGCAACG
    GGCATTCCAA TGGCCATCAG GCCAACTATG CAGAATTTTG TCTTAGAGGT TTTCTTCGTG
    GGTCTACGCA ATTACTCCAA GAGCAGCATG AGTTCTGCTG GTAAGCGGAA AATCAATTTA
    ATGATGGGTG ATCTGGTCCT GTCCAGTGGA CCCTCCACAG CACGCATCCG GAACAGCATA
    AACTTTTTGG TGGCCTACGC ATCTGGAGTG GTTAGTTTGC CAGATCAACA GGATTACTGG
    CCGGCGATCA TAGCCACCGA TGTCCTTCTC TCCGGATTTA GCAGCGAGTC CATTCTGGGT
    GCAGCTCTCA TACCCAATTC ATCAAATTTC CTGCAACGCG ACAGGACCCT CAAGTGTGTG
    TTCAAAAAGG AACAAGAGGC AGCAGAAGCT TCCACCACGG TTAGTGTGGA TGAAGATGAT
    GAGGATGAGG AAGAGGAACT GGAGTGGGAA GAGGAGGAGA CCAAACCCTC CAGGATTAGC
    AGCTGGTGGA TGCGACTGCT CTTCGCGTTC GGTCTGCGTC CAGCCCCCTA TGCCAATCGA
    CACGCTTTAA GGTTCCACGA TGATTTGGAT GACCACGAAT TGGTCGAGGA GCGAGAGTTC
    ACCTGGTGGA CTAAGTTCTA CAACTCGATG TATTGGAGTG CGGCCGAAAT GTGCCACGAA
    CACAAGCACC GCCTGGTTAT CTACTACGAA GAGCTGGAGA AGCAGGCCCA GTTTGGATAC
    CTCCAGGATT GGGCGGTTCC CGTTCAGTTG GTTCATGGCG TGAAGTTTAA AAAGCATGGT
    CCACCCAAAG AAGATATCTA TGCCACCTTA AAGCTGCAAC TCAAGTTGAC CCCTTGTCAG
    TGCCCGGTTC TTGAGGATCC CGGAGGTGGT GGCGACATGG TTCGCAACAT GTCAAATCCC
    ATACATCCTC GACATCAGTC CACACTACAG TCGCTGGGTG AGACCGTAAA ACTATTGGTA
    CGTGTTTATG TAGTCCAGGG GGTTCAGATG CGTCCTCGAG ATGTGAAAGG TGACTCCGAT
    TGCTATGTGA AGCTGTTCCT GGGCGGCAAG ACGTTCTCCG ATAGAGCCCA TTTCTCACCC
    AACCACAGTA ATCCAGTTTT CGGTCGTCTA TTTGAAATGG AGGCTACGCT GCCAGGAGAT
    CATATGCTGC AAGTCATGGT ATATGATCAT GACAAGATCA AGGACGAGGT GATCGGTCAG
    ACAAACATCG ATCTGGAGGA TCGCTGGCGA ACTAGGCATC GTGCCACAGT GGGTTTGGCC
    AATGAGTACA CAAAGAGTGG CTATAATCAT TGGCACGACG TTAAGTTGCC CTCGGAAATT
    CTCATAGATC TGTGCCAGAA AAGAGGCATT CAGGCCCCTT ACTATTATGG TAATGTAATT
    GAGGTGGACG GAATGCTTTT TGGGGACGAA ACTTTGATCT CAAAAGATGA GGAGCTGCAG
    GAGAGACTTA GTTTAGCCGT GCTTAAGAAT ATGGATAAGT TGCCATCCTT TGGTTACAAA
    TTGGTTCCCG AACATGTGGA AACCCGCTCC CTTTGCCGCG ATGATTTTCC AAATGTCGAA
    CAGGGCAAAC TACAACTGTG GATAGAGCTG TTCGAGGCGA ACATCTATGT GCCCAGTCCC
    ATCGATATAA CCCCTGTGCC GCCCGCGGAT TTCGAGGTGC GTGTTGTGGT TAAGAATCTG
    GTCGGGATTC AGGCGGGCGA CAAAAACATT TTTGGCAAAC TAATGAGCGA CGTTTACGTG
    ATAGGATGGT GCGAGGATGA GGACAAGCGC CAATCGACCG ATATCCATTA CCGCTCATTT
    GCAGGAGATG CGGCATTTAA TTGGCGCATG GTCTTCGGCC TCAAGTATTC CCCCAACGAG
    GACCTGATGG TAATCCGGCG AAAGGCGGGG CTGCTGGAGG AAATCGAGTT CAAGAAGCCG
    CCTATTATCT ATCTGCAGGT GTGGGACAAG GATGTGTTAA CCAAGGATGA GTATCTGGCC
    GCACTGGAGC TCAATCTCTC CAACATGTAC GCACCGTATC CAGCTGCCCA GTTATGTAAG
    CCATATCCCA AGAAGAGGAA GCGCATCAAT CTCTTCAAGC GCAAGGTCAT CAACGGCTGG
    TTTCCGCTGC AAAGTAATGC ACATCCATCG CAACGCAGCC AGGCCGCAGT TCAAAATGGC
    AAAATGCAGC TGAGCATCGA AGTCTGCACG GATGTGGAGG CCGTCAACCG GCCGGCTGGT
    CGTGGACAGG ATCCACCGAT GGCCTTGGAG CCGCCTTACA GACCGGACAG CTCGTTCAAT
    CCACTAACGC ATCCTTGCAA ATCCTTTCAG CACATCCTCT GGCCAGTCAT TCGAAAGTAT
    GTCCTGTGGA CTTTGCTCAT ATTGATCGTT ATCCTGGGAT TGGTGCTCTT CTTTTCCAAT
    CTGCCCGCCA AACTCATGAC CATTCCACTT GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa17955
    Clone ID Related Accession (Same CDS sequence) NM_001144448.2
    Accession Version NM_001144448.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4320bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product misfire, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001144448.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTTTCCGT TTCTAAAGTA CAGATTCCAT CCTTTCAAGG GCAAAACGCC GGTGGTCAAG 
    AACACGACGA CGCCCGTGTG GAACTACGAG ATCAACTTTG CCTGGATGTA TCCGTCGGTG
    GCCCAGCGAT TCCTCATCCT AATTTTTGCC CATGAACATC TGCAATGGAA ATGCGTGGCT
    GAGTTTGAAT TGTGTCTTGA GGAGATCGCG TTTAATGGAA CTCCTTCTCT GGGACCCACC
    TACTTGCATC TCTACGATCC TGTCAATCCT ATGATCTATG TTGGTCGTCT TCTAATGGAA
    CTTCGGTCTG AAATGCTAAC GGATTCCCAG CCCACACACA CGCTGTCCTC CAAGACGGTG
    CCAGGTTTGG ATGAGACCCG TTTCTGGCAT GACGATGTCT ATCTGATTGA GTTTCTTCCA
    CTAATGGGTC ACCACATCCT GACCTCGGGT ACGGCATACA AGATCTCGGC GAGATTAGCC
    GAACACACCT CGAACGTGCT GGAGGGTCAA CTAAGGACAC CTTTAGATCG ATTCAAGTCG
    GCAATGAACT CAAAGAATTT CCGGCGGGAG GCACCTTTTC AGTCCTGCAT GTTTCGTGTC
    CGTTTGCCGG ACAATCGAGC CAAGTACGAA AGTGATTTCT TCATGCTGGA TATAGTCAAT
    TATATGCGTT CAGAACTGGA CACCTTCAAG CTCTTTCAAC GCAAATATCC ACTCCAGGAT
    GATGAGCAGG CCAAATGCCT GAAGGTCATT GTGAACAATA TTTTGACCAG AATCAAGGAG
    GGTCTAGATG CCCACCGTTT CGACCACGAC GTGCAACCAC AGCGCACCAC CTGGGATATC
    AATCGGCAGC TGTACCTCCT GGACTATTTC GCCAAGTTGC CACTGGAACT TCAAAATCTT
    AAACAGAAAC TACGCAGCTG CTTTCTGGAA CACCTAGACA CTTCCATTGG CGATGTGGTT
    AATGAGCTAC AGCGTTTGGT AGCTGAAATC GGATCCCTTT CCAGTATGGC ACGCACCCAG
    GACGAGTGGC CAGAATTGGT GCTTTCCTTA AGTGCAAGCG GTAAAGATGT GGGTGTTTGC
    CGATTGAACG CCAAACTCTT TTTGAATCTA CGAAATCAGG ATGCGGATCA GGATCAGGAG
    ACCCTTTGTT GGCGTCTAAA GAGCTTTTCC TTCAAGTCCG CGGGCTGTTC GCACACCTGC
    CCCAATTGTG GTTGCACCAC GGCCATAGTA TCAGGATGTT TATCCATTGT TGTGGAACGG
    GAGCGAAAAC GATTTCTTGC GGTTGTCAAT AAAGAGTGGG CCAACATTGA GCCCATGTTG
    TGGCAGCTGA ATCCTGAACA GACACACTTC CTCTGTCACG TTTACATACA CCAAGCCAAA
    GTTCGTCCTG GTGGTGAAAA AAAGAACATA TGTGACTCAC ATCTGAGAGT CCTGTTTGCC
    GACCAGGCAT GTGAAACGTA TACGTCTCCC GGTACCCTAT CGCCAATTTG GAATGCTGTG
    ATCACATTTA ACTGGGTGTC ACTACCAGGA GGCATTGGTC TCTATCTAAA GAACCCTCCT
    CTACTTTCCT TGGAATTCTA TAATTCGGAA CGCAGTCTAC CAGAAGACCT TGTGGGCATG
    GGAAGCGTGG CCATGAGTGT TATTAGCGAG GATCGGGCAA GTGATTCCAA TTGGGAGGAT
    GCAGGAGTAT TCGGTTGCTC AAAGAATCCG TTGCGAAAAC TACAGAGATT GAAGTACCTG
    ACACCACCTC CTCTCAAATG GGTGCCAATT GCTAGAAAAG GAACTCTACA AGCAGAGGTT
    TTGATGTCAG CCGAGATCAT TCAAATCTCC ATGGAATCTC TGACCACTGG TCAGAATGAT
    AAAAAGGAAC CACAACTTGC AACGGGCATT CCAATGGCCA TCAGGCCAAC TATGCAGAAT
    TTTGTCTTAG AGGTTTTCTT CGTGGGTCTA CGCAATTACT CCAAGAGCAG CATGAGTTCT
    GCTGGTAAGC GGAAAATCAA TTTAATGATG GGTGATCTGG TCCTGTCCAG TGGACCCTCC
    ACAGCACGCA TCCGGAACAG CATAAACTTT TTGGTGGCCT ACGCATCTGG AGTGGTTAGT
    TTGCCAGATC AACAGGATTA CTGGCCGGCG ATCATAGCCA CCGATGTCCT TCTCTCCGGA
    TTTAGCAGCG AGTCCATTCT GGGTGCAGCT CTCATACCCA ATTCATCAAA TTTCCTGCAA
    CGCGACAGGA CCCTCAAGTG TGTGTTCAAA AAGGAACAAG AGGCAGCAGA AGCTTCCACC
    ACGGTTAGTG TGGATGAAGA TGATGAGGAT GAGGAAGAGG AACTGGAGTG GGAAGAGGAG
    GAGACCAAAC CCTCCAGGAT TAGCAGCTGG TGGATGCGAC TGCTCTTCGC GTTCGGTCTG
    CGTCCAGCCC CCTATGCCAA TCGACACGCT TTAAGGTTCC ACGATGATTT GGATGACCAC
    GAATTGGTCG AGGAGCGAGA GTTCACCTGG TGGACTAAGT TCTACAACTC GATGTATTGG
    AGTGCGGCCG AAATGTGCCA CGAACACAAG CACCGCCTGG TTATCTACTA CGAAGAGCTG
    GAGAAGCAGG CCCAGTTTGG ATACCTCCAG GATTGGGCGG TTCCCGTTCA GTTGGTTCAT
    GGCGTGAAGT TTAAAAAGCA TGGTCCACCC AAAGAAGATA TCTATGCCAC CTTAAAGCTG
    CAACTCAAGT TGACCCCTTG TCAGTGCCCG GTTCTTGAGG ATCCCGGAGG TGGTGGCGAC
    ATGGTTCGCA ACATGTCAAA TCCCATACAT CCTCGACATC AGTCCACACT ACAGTCGCTG
    GGTGAGACCG TAAAACTATT GGTACGTGTT TATGTAGTCC AGGGGGTTCA GATGCGTCCT
    CGAGATGTGA AAGGTGACTC CGATTGCTAT GTGAAGCTGT TCCTGGGCGG CAAGACGTTC
    TCCGATAGAG CCCATTTCTC ACCCAACCAC AGTAATCCAG TTTTCGGTCG TCTATTTGAA
    ATGGAGGCTA CGCTGCCAGG AGATCATATG CTGCAAGTCA TGGTATATGA TCATGACAAG
    ATCAAGGACG AGGTGATCGG TCAGACAAAC ATCGATCTGG AGGATCGCTG GCGAACTAGG
    CATCGTGCCA CAGTGGGTTT GGCCAATGAG TACACAAAGA GTGGCTATAA TCATTGGCAC
    GACGTTAAGT TGCCCTCGGA AATTCTCATA GATCTGTGCC AGAAAAGAGG CATTCAGGCC
    CCTTACTATT ATGGTAATGT AATTGAGGTG GACGGAATGC TTTTTGGGGA CGAAACTTTG
    ATCTCAAAAG ATGAGGAGCT GCAGGAGAGA CTTAGTTTAG CCGTGCTTAA GAATATGGAT
    AAGTTGCCAT CCTTTGGTTA CAAATTGGTT CCCGAACATG TGGAAACCCG CTCCCTTTGC
    CGCGATGATT TTCCAAATGT CGAACAGGGC AAACTACAAC TGTGGATAGA GCTGTTCGAG
    GCGAACATCT ATGTGCCCAG TCCCATCGAT ATAACCCCTG TGCCGCCCGC GGATTTCGAG
    GTGCGTGTTG TGGTTAAGAA TCTGGTCGGG ATTCAGGCGG GCGACAAAAA CATTTTTGGC
    AAACTAATGA GCGACGTTTA CGTGATAGGA TGGTGCGAGG ATGAGGACAA GCGCCAATCG
    ACCGATATCC ATTACCGCTC ATTTGCAGGA GATGCGGCAT TTAATTGGCG CATGGTCTTC
    GGCCTCAAGT ATTCCCCCAA CGAGGACCTG ATGGTAATCC GGCGAAAGGC GGGGCTGCTG
    GAGGAAATCG AGTTCAAGAA GCCGCCTATT ATCTATCTGC AGGTGTGGGA CAAGGATGTG
    TTAACCAAGG ATGAGTATCT GGCCGCACTG GAGCTCAATC TCTCCAACAT GTACGCACCG
    TATCCAGCTG CCCAGTTATG TAAGCCATAT CCCAAGAAGA GGAAGCGCAT CAATCTCTTC
    AAGCGCAAGG TCATCAACGG CTGGTTTCCG CTGCAAAGTA ATGCACATCC ATCGCAACGC
    AGCCAGGCCG CAGTTCAAAA TGGCAAAATG CAGCTGAGCA TCGAAGTCTG CACGGATGTG
    GAGGCCGTCA ACCGGCCGGC TGGTCGTGGA CAGGATCCAC CGATGGCCTT GGAGCCGCCT
    TACAGACCGG ACAGCTCGTT CAATCCACTA ACGCATCCTT GCAAATCCTT TCAGCACATC
    CTCTGGCCAG TCATTCGAAA GTATGTCCTG TGGACTTTGC TCATATTGAT CGTTATCCTG
    GGATTGGTGC TCTTCTTTTC CAATCTGCCC GCCAAACTCA TGACCATTCC ACTTGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137920.1
    CDS800..5119
    Translation

    Target ORF information:

    RefSeq Version NM_001144448.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster misfire (mfr), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144448.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTTTCCGT TTCTAAAGTA CAGATTCCAT CCTTTCAAGG GCAAAACGCC GGTGGTCAAG 
    AACACGACGA CGCCCGTGTG GAACTACGAG ATCAACTTTG CCTGGATGTA TCCGTCGGTG
    GCCCAGCGAT TCCTCATCCT AATTTTTGCC CATGAACATC TGCAATGGAA ATGCGTGGCT
    GAGTTTGAAT TGTGTCTTGA GGAGATCGCG TTTAATGGAA CTCCTTCTCT GGGACCCACC
    TACTTGCATC TCTACGATCC TGTCAATCCT ATGATCTATG TTGGTCGTCT TCTAATGGAA
    CTTCGGTCTG AAATGCTAAC GGATTCCCAG CCCACACACA CGCTGTCCTC CAAGACGGTG
    CCAGGTTTGG ATGAGACCCG TTTCTGGCAT GACGATGTCT ATCTGATTGA GTTTCTTCCA
    CTAATGGGTC ACCACATCCT GACCTCGGGT ACGGCATACA AGATCTCGGC GAGATTAGCC
    GAACACACCT CGAACGTGCT GGAGGGTCAA CTAAGGACAC CTTTAGATCG ATTCAAGTCG
    GCAATGAACT CAAAGAATTT CCGGCGGGAG GCACCTTTTC AGTCCTGCAT GTTTCGTGTC
    CGTTTGCCGG ACAATCGAGC CAAGTACGAA AGTGATTTCT TCATGCTGGA TATAGTCAAT
    TATATGCGTT CAGAACTGGA CACCTTCAAG CTCTTTCAAC GCAAATATCC ACTCCAGGAT
    GATGAGCAGG CCAAATGCCT GAAGGTCATT GTGAACAATA TTTTGACCAG AATCAAGGAG
    GGTCTAGATG CCCACCGTTT CGACCACGAC GTGCAACCAC AGCGCACCAC CTGGGATATC
    AATCGGCAGC TGTACCTCCT GGACTATTTC GCCAAGTTGC CACTGGAACT TCAAAATCTT
    AAACAGAAAC TACGCAGCTG CTTTCTGGAA CACCTAGACA CTTCCATTGG CGATGTGGTT
    AATGAGCTAC AGCGTTTGGT AGCTGAAATC GGATCCCTTT CCAGTATGGC ACGCACCCAG
    GACGAGTGGC CAGAATTGGT GCTTTCCTTA AGTGCAAGCG GTAAAGATGT GGGTGTTTGC
    CGATTGAACG CCAAACTCTT TTTGAATCTA CGAAATCAGG ATGCGGATCA GGATCAGGAG
    ACCCTTTGTT GGCGTCTAAA GAGCTTTTCC TTCAAGTCCG CGGGCTGTTC GCACACCTGC
    CCCAATTGTG GTTGCACCAC GGCCATAGTA TCAGGATGTT TATCCATTGT TGTGGAACGG
    GAGCGAAAAC GATTTCTTGC GGTTGTCAAT AAAGAGTGGG CCAACATTGA GCCCATGTTG
    TGGCAGCTGA ATCCTGAACA GACACACTTC CTCTGTCACG TTTACATACA CCAAGCCAAA
    GTTCGTCCTG GTGGTGAAAA AAAGAACATA TGTGACTCAC ATCTGAGAGT CCTGTTTGCC
    GACCAGGCAT GTGAAACGTA TACGTCTCCC GGTACCCTAT CGCCAATTTG GAATGCTGTG
    ATCACATTTA ACTGGGTGTC ACTACCAGGA GGCATTGGTC TCTATCTAAA GAACCCTCCT
    CTACTTTCCT TGGAATTCTA TAATTCGGAA CGCAGTCTAC CAGAAGACCT TGTGGGCATG
    GGAAGCGTGG CCATGAGTGT TATTAGCGAG GATCGGGCAA GTGATTCCAA TTGGGAGGAT
    GCAGGAGTAT TCGGTTGCTC AAAGAATCCG TTGCGAAAAC TACAGAGATT GAAGTACCTG
    ACACCACCTC CTCTCAAATG GGTGCCAATT GCTAGAAAAG GAACTCTACA AGCAGAGGTT
    TTGATGTCAG CCGAGATCAT TCAAATCTCC ATGGAATCTC TGACCACTGG TCAGAATGAT
    AAAAAGGAAC CACAACTTGC AACGGGCATT CCAATGGCCA TCAGGCCAAC TATGCAGAAT
    TTTGTCTTAG AGGTTTTCTT CGTGGGTCTA CGCAATTACT CCAAGAGCAG CATGAGTTCT
    GCTGGTAAGC GGAAAATCAA TTTAATGATG GGTGATCTGG TCCTGTCCAG TGGACCCTCC
    ACAGCACGCA TCCGGAACAG CATAAACTTT TTGGTGGCCT ACGCATCTGG AGTGGTTAGT
    TTGCCAGATC AACAGGATTA CTGGCCGGCG ATCATAGCCA CCGATGTCCT TCTCTCCGGA
    TTTAGCAGCG AGTCCATTCT GGGTGCAGCT CTCATACCCA ATTCATCAAA TTTCCTGCAA
    CGCGACAGGA CCCTCAAGTG TGTGTTCAAA AAGGAACAAG AGGCAGCAGA AGCTTCCACC
    ACGGTTAGTG TGGATGAAGA TGATGAGGAT GAGGAAGAGG AACTGGAGTG GGAAGAGGAG
    GAGACCAAAC CCTCCAGGAT TAGCAGCTGG TGGATGCGAC TGCTCTTCGC GTTCGGTCTG
    CGTCCAGCCC CCTATGCCAA TCGACACGCT TTAAGGTTCC ACGATGATTT GGATGACCAC
    GAATTGGTCG AGGAGCGAGA GTTCACCTGG TGGACTAAGT TCTACAACTC GATGTATTGG
    AGTGCGGCCG AAATGTGCCA CGAACACAAG CACCGCCTGG TTATCTACTA CGAAGAGCTG
    GAGAAGCAGG CCCAGTTTGG ATACCTCCAG GATTGGGCGG TTCCCGTTCA GTTGGTTCAT
    GGCGTGAAGT TTAAAAAGCA TGGTCCACCC AAAGAAGATA TCTATGCCAC CTTAAAGCTG
    CAACTCAAGT TGACCCCTTG TCAGTGCCCG GTTCTTGAGG ATCCCGGAGG TGGTGGCGAC
    ATGGTTCGCA ACATGTCAAA TCCCATACAT CCTCGACATC AGTCCACACT ACAGTCGCTG
    GGTGAGACCG TAAAACTATT GGTACGTGTT TATGTAGTCC AGGGGGTTCA GATGCGTCCT
    CGAGATGTGA AAGGTGACTC CGATTGCTAT GTGAAGCTGT TCCTGGGCGG CAAGACGTTC
    TCCGATAGAG CCCATTTCTC ACCCAACCAC AGTAATCCAG TTTTCGGTCG TCTATTTGAA
    ATGGAGGCTA CGCTGCCAGG AGATCATATG CTGCAAGTCA TGGTATATGA TCATGACAAG
    ATCAAGGACG AGGTGATCGG TCAGACAAAC ATCGATCTGG AGGATCGCTG GCGAACTAGG
    CATCGTGCCA CAGTGGGTTT GGCCAATGAG TACACAAAGA GTGGCTATAA TCATTGGCAC
    GACGTTAAGT TGCCCTCGGA AATTCTCATA GATCTGTGCC AGAAAAGAGG CATTCAGGCC
    CCTTACTATT ATGGTAATGT AATTGAGGTG GACGGAATGC TTTTTGGGGA CGAAACTTTG
    ATCTCAAAAG ATGAGGAGCT GCAGGAGAGA CTTAGTTTAG CCGTGCTTAA GAATATGGAT
    AAGTTGCCAT CCTTTGGTTA CAAATTGGTT CCCGAACATG TGGAAACCCG CTCCCTTTGC
    CGCGATGATT TTCCAAATGT CGAACAGGGC AAACTACAAC TGTGGATAGA GCTGTTCGAG
    GCGAACATCT ATGTGCCCAG TCCCATCGAT ATAACCCCTG TGCCGCCCGC GGATTTCGAG
    GTGCGTGTTG TGGTTAAGAA TCTGGTCGGG ATTCAGGCGG GCGACAAAAA CATTTTTGGC
    AAACTAATGA GCGACGTTTA CGTGATAGGA TGGTGCGAGG ATGAGGACAA GCGCCAATCG
    ACCGATATCC ATTACCGCTC ATTTGCAGGA GATGCGGCAT TTAATTGGCG CATGGTCTTC
    GGCCTCAAGT ATTCCCCCAA CGAGGACCTG ATGGTAATCC GGCGAAAGGC GGGGCTGCTG
    GAGGAAATCG AGTTCAAGAA GCCGCCTATT ATCTATCTGC AGGTGTGGGA CAAGGATGTG
    TTAACCAAGG ATGAGTATCT GGCCGCACTG GAGCTCAATC TCTCCAACAT GTACGCACCG
    TATCCAGCTG CCCAGTTATG TAAGCCATAT CCCAAGAAGA GGAAGCGCAT CAATCTCTTC
    AAGCGCAAGG TCATCAACGG CTGGTTTCCG CTGCAAAGTA ATGCACATCC ATCGCAACGC
    AGCCAGGCCG CAGTTCAAAA TGGCAAAATG CAGCTGAGCA TCGAAGTCTG CACGGATGTG
    GAGGCCGTCA ACCGGCCGGC TGGTCGTGGA CAGGATCCAC CGATGGCCTT GGAGCCGCCT
    TACAGACCGG ACAGCTCGTT CAATCCACTA ACGCATCCTT GCAAATCCTT TCAGCACATC
    CTCTGGCCAG TCATTCGAAA GTATGTCCTG TGGACTTTGC TCATATTGAT CGTTATCCTG
    GGATTGGTGC TCTTCTTTTC CAATCTGCCC GCCAAACTCA TGACCATTCC ACTTGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.