dally cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dally
Entrez Gene ID	39013
Full Name	division abnormally delayed
Synonyms	CG4974,CT15952,Dally,Dmel\CG4974,anon-EST:Liang-1.63,clone 1.63,gem,l(3)06464,l(3)SG18
General protein information	Preferred Names division abnormally delayed Names CG4974 gene product from transcript CG4974-RB CG4974-PA CG4974-PB dally-PA dally-PB genitalia missing lethal (3) SG18
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66E1-66E3\|3-31.9 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_079259.4	NP_523983.1	division abnormally delayed, isoform A
NM_001259756.2	NP_001246685.1	division abnormally delayed, isoform B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GPC5	XP_542637.4
Mus musculus (house mouse)	Gpc5	NP_780709.1
Rattus norvegicus (Norway rat)	Gpc5	NP_001100755.2
Drosophila melanogaster (fruit fly)	dally	NP_001246685.1
Homo sapiens (human)	GPC5	NP_004457.1
	GPC5	XP_002800848.1
Gallus gallus (chicken)	GPC5	XP_004938641.1
Danio rerio (zebrafish)	LOC559454	XP_001920549.4
Bos taurus (cattle)	GPC5	NP_001095540.1
Pan troglodytes (chimpanzee)	GPC5	XP_509696.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005799	XP_003435729.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ECM over planar cell polarity mutant cells had reduced levels of laminin, Dally and Dlp, and whereas Dpp-receiving ASP cytonemes navigated in the Dally layer and required Dally (but not Dlp), FGF-receiving ASP cytonemes navigated in the Dlp layer, requiring Dlp (but not Dally).
Title: Cells must express components of the planar cell polarity system and extracellular matrix to support cytonemes.

Autonomous and nonautonomous effects on Drosophila tissue growth rely on the up-regulation of the proteoglycan Dally. Dally also contributes to integrating nutrient sensing and organ scaling, the fitting of pattern to size.
Title: Dally Proteoglycan Mediates the Autonomous and Nonautonomous Effects on Tissue Growth Caused by Activation of the PI3K and TOR Pathways.

analysis of balancing between hedgehog, a retention and release equilibrium given by Dally, Ihog, Boi and shifted/DmWif
Title: Balancing Hedgehog, a retention and release equilibrium given by Dally, Ihog, Boi and shifted/DmWif.

data suggest that Dally and Dlp are essential regulators for Upd distribution and JAK/STAT signaling activity in eye development
Title: Drosophila glypicans Dally and Dally-like are essential regulators for JAK/STAT signaling and Unpaired distribution in eye development.

findings demonstrate that the glypican dally regulates formation of the follicular gradient of the Upd morphogen, Upd
Title: Glypicans regulate JAK/STAT signaling and distribution of the Unpaired morphogen.

The following dally gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dally cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17946	NM_001259756.2 Latest version!	Drosophila melanogaster division abnormally delayed (dally), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa17946	NM_079259.4 Latest version!	Drosophila melanogaster division abnormally delayed (dally), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dally ( NM_079259.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523983.1 Drosophila melanogaster division abnormally delayed (dally), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17946
Clone ID Related Accession (Same CDS sequence)	NM_079259.4 , NM_001259756.2
Accession Version	NM_079259.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	division abnormally delayed, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079259.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGCCA GGAGCGTGCG GCTAGCGCAG CTTTTGCTAT TTACCCTGCT CTGCGGCTTC 
GTCGGCCTGT CAGCCGCCAA GCACCTGGAC CTGGATGGTA TCCACCACCA CCAGCACCAC 
CTGCACAGCG CCACCACGCA CCACAGACGT CGCCTACAGC GGGACTCGCG GGCGAAGGAC 
GCCGTTGGGG GATCGACCCA CCAGTGTGAT GCCGTCAAGA GCTACTTCGA ATCGATCGAC 
ATCAAGTCGA GTGGGACTTA CAGCGAAAAA GGCGCCATTT GTGGCGGAAA CTGCTGCAAC 
AATGCCACGG AGCTGGAGCT GCGGGACAAA GCCGCCGGAA TGTTCGAGCA ACTGCTTCAT 
CATCACACCA GCAGCCTGCG CGGAGTCCTC GAGACGAATG CCAAACAGTT CCAAAGTCAC 
GTCCTGGAAT TGGCGCAGAT TTCGGAGAAC ATGACCCACT CGCTCTTTTC GAAGGTTTAT 
ACCCGTATGG TGCCTTCATC GCGTATGATG ATCCATCAAC TGTACACCGA AATCATGAAC 
CATCTGATTT ACACCTCCAA CTATACGAAC AGCAATGGCC AACTGGGCAG GCGAGGAATT 
GGCAGTGTCC AGAGCAACTT GGAGGAGGCT GTCAGGCACT TCTTTGTTCA GTTGTTCCCC 
GTGGCCTACC ACCAGATGGT CCATCTGTCC AAGAACAATT TGGGTGACTT GCACGAGGAC 
TACGTTAACT GCCTGCAGCA CAACTTCGAC GAGATGCATC CATTCGGGGA CATTCCCCAG 
CAAGTGCAGT CCAACTTGGG CAAGAGTGTC CATATGTCCA ATGTCTTCAT GAATGCCCTG 
CTCCAGGCCG CCGAGGTCCT CAGCGAAGCG GATGCCCTTT ATGGCGAACA ACTGACGGAC 
ACGTGCAAAC TGCATCTCCT CAAGATGCAC TACTGCCCCA ACTGCAATGG ACACCATTCA 
AGTTCTAGGA GCGAAACGAA ATTGTGCTAC GGCTACTGCA AGAACGTAAT GCGCGGCTGT 
TCGGCGGAAT ATGCCGGACT ACTGGACAGT CCCTGGTCAG GTGTAGTCGA CTCCCTGAAC 
AATCTGGTCA CCACCCATAT TCTCTCGGAC ACCGGCATCA TTAATGTCAT CAAACACCTG 
CAGACTTATT TCTCTGAGGC AATCATGGCT GCCATGCACA ATGGGCCTGA ACTGGAAAAA 
AAGGTAAAGA AGACATGTGG CACGCCCAGC CTGACGCCCT ATTCGTCCGG AGAACCGGAT 
GCGCGGCCTC CGCCCCACAA GAACAACGTA AAGTGGGCCA CTGACCCGGA TCCGGGCATG 
GTCCTCTTCC TGTCCACCAT CGACAAGAGC AAGGAGTTCT ACACGACCAT CGTGGACAAC 
TTCTGCGATG AGCAACAGCA CTCTCGTGAC GATCACTCCT GCTGGTCAGG CGATCGTTTC 
GGGGACTACA CTCAGCTGCT GATTAATCCG GGCACTGATA GCCAGCGATA TAATCCCGAG 
GTGCCCTTCA ATGCGAAGGC TCAGACCGGC AAGCTGAACG AGCTGGTCGA CAAGCTCTTC 
AAGATTCGCA AGAGCATTGG AGCTGCGGCA CCTTCCAACA GCATACAGGC GACGCACGAC 
ATCCAGAATG ATATGGGTGA GGGCTCCGGC GGCGGAGAAG GTCAGATCGG CGACGATGAG 
GAGGAGTATG GAGGCGCACA TGGTTCCGGA GATGGTTCCG GCGATGGACC CCATACGCCC 
ATCGAGGAAT CCGAAGGCAC CACCACCAAC GAAGTGGAGT CGCGTGACAG CGGCAAGACC 
AGTGGGTCCA ACCCACTGGA AGGCACCGCG ACGTGGATGC TCCTCACTCT AGTCACAATG 
CTCTTTAGTA GCTGTAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523983.1
CDS	729..2609
Translation	MAARSVRLAQLLLFTLLCGFVGLSAAKHLDLDGIHHHQHHLHSATTHHRRRLQRDSRAKDAVGGSTHQCDAVKSYFESIDIKSSGTYSEKGAICGGNCCNNATELELRDKAAGMFEQLLHHHTSSLRGVLETNAKQFQSHVLELAQISENMTHSLFSKVYTRMVPSSRMMIHQLYTEIMNHLIYTSNYTNSNGQLGRRGIGSVQSNLEEAVRHFFVQLFPVAYHQMVHLSKNNLGDLHEDYVNCLQHNFDEMHPFGDIPQQVQSNLGKSVHMSNVFMNALLQAAEVLSEADALYGEQLTDTCKLHLLKMHYCPNCNGHHSSSRSETKLCYGYCKNVMRGCSAEYAGLLDSPWSGVVDSLNNLVTTHILSDTGIINVIKHLQTYFSEAIMAAMHNGPELEKKVKKTCGTPSLTPYSSGEPDARPPPHKNNVKWATDPDPGMVLFLSTIDKSKEFYTTIVDNFCDEQQHSRDDHSCWSGDRFGDYTQLLINPGTDSQRYNPEVPFNAKAQTGKLNELVDKLFKIRKSIGAAAPSNSIQATHDIQNDMGEGSGGGEGQIGDDEEEYGGAHGSGDGSGDGPHTPIEESEGTTTNEVESRDSGKTSGSNPLEGTATWMLLTLVTMLFSSCS

Target ORF information:

RefSeq Version	NM_079259.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster division abnormally delayed (dally), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079259.4

ORF Insert Sequence:

ATGGCGGCCA GGAGCGTGCG GCTAGCGCAG CTTTTGCTAT TTACCCTGCT CTGCGGCTTC 
GTCGGCCTGT CAGCCGCCAA GCACCTGGAC CTGGATGGTA TCCACCACCA CCAGCACCAC 
CTGCACAGCG CCACCACGCA CCACAGACGT CGCCTACAGC GGGACTCGCG GGCGAAGGAC 
GCCGTTGGGG GATCGACCCA CCAGTGTGAT GCCGTCAAGA GCTACTTCGA ATCGATCGAC 
ATCAAGTCGA GTGGGACTTA CAGCGAAAAA GGCGCCATTT GTGGCGGAAA CTGCTGCAAC 
AATGCCACGG AGCTGGAGCT GCGGGACAAA GCCGCCGGAA TGTTCGAGCA ACTGCTTCAT 
CATCACACCA GCAGCCTGCG CGGAGTCCTC GAGACGAATG CCAAACAGTT CCAAAGTCAC 
GTCCTGGAAT TGGCGCAGAT TTCGGAGAAC ATGACCCACT CGCTCTTTTC GAAGGTTTAT 
ACCCGTATGG TGCCTTCATC GCGTATGATG ATCCATCAAC TGTACACCGA AATCATGAAC 
CATCTGATTT ACACCTCCAA CTATACGAAC AGCAATGGCC AACTGGGCAG GCGAGGAATT 
GGCAGTGTCC AGAGCAACTT GGAGGAGGCT GTCAGGCACT TCTTTGTTCA GTTGTTCCCC 
GTGGCCTACC ACCAGATGGT CCATCTGTCC AAGAACAATT TGGGTGACTT GCACGAGGAC 
TACGTTAACT GCCTGCAGCA CAACTTCGAC GAGATGCATC CATTCGGGGA CATTCCCCAG 
CAAGTGCAGT CCAACTTGGG CAAGAGTGTC CATATGTCCA ATGTCTTCAT GAATGCCCTG 
CTCCAGGCCG CCGAGGTCCT CAGCGAAGCG GATGCCCTTT ATGGCGAACA ACTGACGGAC 
ACGTGCAAAC TGCATCTCCT CAAGATGCAC TACTGCCCCA ACTGCAATGG ACACCATTCA 
AGTTCTAGGA GCGAAACGAA ATTGTGCTAC GGCTACTGCA AGAACGTAAT GCGCGGCTGT 
TCGGCGGAAT ATGCCGGACT ACTGGACAGT CCCTGGTCAG GTGTAGTCGA CTCCCTGAAC 
AATCTGGTCA CCACCCATAT TCTCTCGGAC ACCGGCATCA TTAATGTCAT CAAACACCTG 
CAGACTTATT TCTCTGAGGC AATCATGGCT GCCATGCACA ATGGGCCTGA ACTGGAAAAA 
AAGGTAAAGA AGACATGTGG CACGCCCAGC CTGACGCCCT ATTCGTCCGG AGAACCGGAT 
GCGCGGCCTC CGCCCCACAA GAACAACGTA AAGTGGGCCA CTGACCCGGA TCCGGGCATG 
GTCCTCTTCC TGTCCACCAT CGACAAGAGC AAGGAGTTCT ACACGACCAT CGTGGACAAC 
TTCTGCGATG AGCAACAGCA CTCTCGTGAC GATCACTCCT GCTGGTCAGG CGATCGTTTC 
GGGGACTACA CTCAGCTGCT GATTAATCCG GGCACTGATA GCCAGCGATA TAATCCCGAG 
GTGCCCTTCA ATGCGAAGGC TCAGACCGGC AAGCTGAACG AGCTGGTCGA CAAGCTCTTC 
AAGATTCGCA AGAGCATTGG AGCTGCGGCA CCTTCCAACA GCATACAGGC GACGCACGAC 
ATCCAGAATG ATATGGGTGA GGGCTCCGGC GGCGGAGAAG GTCAGATCGG CGACGATGAG 
GAGGAGTATG GAGGCGCACA TGGTTCCGGA GATGGTTCCG GCGATGGACC CCATACGCCC 
ATCGAGGAAT CCGAAGGCAC CACCACCAAC GAAGTGGAGT CGCGTGACAG CGGCAAGACC 
AGTGGGTCCA ACCCACTGGA AGGCACCGCG ACGTGGATGC TCCTCACTCT AGTCACAATG 
CTCTTTAGTA GCTGTAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dally ( NM_001259756.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246685.1 Drosophila melanogaster division abnormally delayed (dally), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17946
Clone ID Related Accession (Same CDS sequence)	NM_079259.4 , NM_001259756.2
Accession Version	NM_001259756.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	division abnormally delayed, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001259756.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGCCA GGAGCGTGCG GCTAGCGCAG CTTTTGCTAT TTACCCTGCT CTGCGGCTTC 
GTCGGCCTGT CAGCCGCCAA GCACCTGGAC CTGGATGGTA TCCACCACCA CCAGCACCAC 
CTGCACAGCG CCACCACGCA CCACAGACGT CGCCTACAGC GGGACTCGCG GGCGAAGGAC 
GCCGTTGGGG GATCGACCCA CCAGTGTGAT GCCGTCAAGA GCTACTTCGA ATCGATCGAC 
ATCAAGTCGA GTGGGACTTA CAGCGAAAAA GGCGCCATTT GTGGCGGAAA CTGCTGCAAC 
AATGCCACGG AGCTGGAGCT GCGGGACAAA GCCGCCGGAA TGTTCGAGCA ACTGCTTCAT 
CATCACACCA GCAGCCTGCG CGGAGTCCTC GAGACGAATG CCAAACAGTT CCAAAGTCAC 
GTCCTGGAAT TGGCGCAGAT TTCGGAGAAC ATGACCCACT CGCTCTTTTC GAAGGTTTAT 
ACCCGTATGG TGCCTTCATC GCGTATGATG ATCCATCAAC TGTACACCGA AATCATGAAC 
CATCTGATTT ACACCTCCAA CTATACGAAC AGCAATGGCC AACTGGGCAG GCGAGGAATT 
GGCAGTGTCC AGAGCAACTT GGAGGAGGCT GTCAGGCACT TCTTTGTTCA GTTGTTCCCC 
GTGGCCTACC ACCAGATGGT CCATCTGTCC AAGAACAATT TGGGTGACTT GCACGAGGAC 
TACGTTAACT GCCTGCAGCA CAACTTCGAC GAGATGCATC CATTCGGGGA CATTCCCCAG 
CAAGTGCAGT CCAACTTGGG CAAGAGTGTC CATATGTCCA ATGTCTTCAT GAATGCCCTG 
CTCCAGGCCG CCGAGGTCCT CAGCGAAGCG GATGCCCTTT ATGGCGAACA ACTGACGGAC 
ACGTGCAAAC TGCATCTCCT CAAGATGCAC TACTGCCCCA ACTGCAATGG ACACCATTCA 
AGTTCTAGGA GCGAAACGAA ATTGTGCTAC GGCTACTGCA AGAACGTAAT GCGCGGCTGT 
TCGGCGGAAT ATGCCGGACT ACTGGACAGT CCCTGGTCAG GTGTAGTCGA CTCCCTGAAC 
AATCTGGTCA CCACCCATAT TCTCTCGGAC ACCGGCATCA TTAATGTCAT CAAACACCTG 
CAGACTTATT TCTCTGAGGC AATCATGGCT GCCATGCACA ATGGGCCTGA ACTGGAAAAA 
AAGGTAAAGA AGACATGTGG CACGCCCAGC CTGACGCCCT ATTCGTCCGG AGAACCGGAT 
GCGCGGCCTC CGCCCCACAA GAACAACGTA AAGTGGGCCA CTGACCCGGA TCCGGGCATG 
GTCCTCTTCC TGTCCACCAT CGACAAGAGC AAGGAGTTCT ACACGACCAT CGTGGACAAC 
TTCTGCGATG AGCAACAGCA CTCTCGTGAC GATCACTCCT GCTGGTCAGG CGATCGTTTC 
GGGGACTACA CTCAGCTGCT GATTAATCCG GGCACTGATA GCCAGCGATA TAATCCCGAG 
GTGCCCTTCA ATGCGAAGGC TCAGACCGGC AAGCTGAACG AGCTGGTCGA CAAGCTCTTC 
AAGATTCGCA AGAGCATTGG AGCTGCGGCA CCTTCCAACA GCATACAGGC GACGCACGAC 
ATCCAGAATG ATATGGGTGA GGGCTCCGGC GGCGGAGAAG GTCAGATCGG CGACGATGAG 
GAGGAGTATG GAGGCGCACA TGGTTCCGGA GATGGTTCCG GCGATGGACC CCATACGCCC 
ATCGAGGAAT CCGAAGGCAC CACCACCAAC GAAGTGGAGT CGCGTGACAG CGGCAAGACC 
AGTGGGTCCA ACCCACTGGA AGGCACCGCG ACGTGGATGC TCCTCACTCT AGTCACAATG 
CTCTTTAGTA GCTGTAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246685.1
CDS	729..2609
Translation	MAARSVRLAQLLLFTLLCGFVGLSAAKHLDLDGIHHHQHHLHSATTHHRRRLQRDSRAKDAVGGSTHQCDAVKSYFESIDIKSSGTYSEKGAICGGNCCNNATELELRDKAAGMFEQLLHHHTSSLRGVLETNAKQFQSHVLELAQISENMTHSLFSKVYTRMVPSSRMMIHQLYTEIMNHLIYTSNYTNSNGQLGRRGIGSVQSNLEEAVRHFFVQLFPVAYHQMVHLSKNNLGDLHEDYVNCLQHNFDEMHPFGDIPQQVQSNLGKSVHMSNVFMNALLQAAEVLSEADALYGEQLTDTCKLHLLKMHYCPNCNGHHSSSRSETKLCYGYCKNVMRGCSAEYAGLLDSPWSGVVDSLNNLVTTHILSDTGIINVIKHLQTYFSEAIMAAMHNGPELEKKVKKTCGTPSLTPYSSGEPDARPPPHKNNVKWATDPDPGMVLFLSTIDKSKEFYTTIVDNFCDEQQHSRDDHSCWSGDRFGDYTQLLINPGTDSQRYNPEVPFNAKAQTGKLNELVDKLFKIRKSIGAAAPSNSIQATHDIQNDMGEGSGGGEGQIGDDEEEYGGAHGSGDGSGDGPHTPIEESEGTTTNEVESRDSGKTSGSNPLEGTATWMLLTLVTMLFSSCS

Target ORF information:

RefSeq Version	NM_001259756.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster division abnormally delayed (dally), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259756.2

ORF Insert Sequence:

ATGGCGGCCA GGAGCGTGCG GCTAGCGCAG CTTTTGCTAT TTACCCTGCT CTGCGGCTTC 
GTCGGCCTGT CAGCCGCCAA GCACCTGGAC CTGGATGGTA TCCACCACCA CCAGCACCAC 
CTGCACAGCG CCACCACGCA CCACAGACGT CGCCTACAGC GGGACTCGCG GGCGAAGGAC 
GCCGTTGGGG GATCGACCCA CCAGTGTGAT GCCGTCAAGA GCTACTTCGA ATCGATCGAC 
ATCAAGTCGA GTGGGACTTA CAGCGAAAAA GGCGCCATTT GTGGCGGAAA CTGCTGCAAC 
AATGCCACGG AGCTGGAGCT GCGGGACAAA GCCGCCGGAA TGTTCGAGCA ACTGCTTCAT 
CATCACACCA GCAGCCTGCG CGGAGTCCTC GAGACGAATG CCAAACAGTT CCAAAGTCAC 
GTCCTGGAAT TGGCGCAGAT TTCGGAGAAC ATGACCCACT CGCTCTTTTC GAAGGTTTAT 
ACCCGTATGG TGCCTTCATC GCGTATGATG ATCCATCAAC TGTACACCGA AATCATGAAC 
CATCTGATTT ACACCTCCAA CTATACGAAC AGCAATGGCC AACTGGGCAG GCGAGGAATT 
GGCAGTGTCC AGAGCAACTT GGAGGAGGCT GTCAGGCACT TCTTTGTTCA GTTGTTCCCC 
GTGGCCTACC ACCAGATGGT CCATCTGTCC AAGAACAATT TGGGTGACTT GCACGAGGAC 
TACGTTAACT GCCTGCAGCA CAACTTCGAC GAGATGCATC CATTCGGGGA CATTCCCCAG 
CAAGTGCAGT CCAACTTGGG CAAGAGTGTC CATATGTCCA ATGTCTTCAT GAATGCCCTG 
CTCCAGGCCG CCGAGGTCCT CAGCGAAGCG GATGCCCTTT ATGGCGAACA ACTGACGGAC 
ACGTGCAAAC TGCATCTCCT CAAGATGCAC TACTGCCCCA ACTGCAATGG ACACCATTCA 
AGTTCTAGGA GCGAAACGAA ATTGTGCTAC GGCTACTGCA AGAACGTAAT GCGCGGCTGT 
TCGGCGGAAT ATGCCGGACT ACTGGACAGT CCCTGGTCAG GTGTAGTCGA CTCCCTGAAC 
AATCTGGTCA CCACCCATAT TCTCTCGGAC ACCGGCATCA TTAATGTCAT CAAACACCTG 
CAGACTTATT TCTCTGAGGC AATCATGGCT GCCATGCACA ATGGGCCTGA ACTGGAAAAA 
AAGGTAAAGA AGACATGTGG CACGCCCAGC CTGACGCCCT ATTCGTCCGG AGAACCGGAT 
GCGCGGCCTC CGCCCCACAA GAACAACGTA AAGTGGGCCA CTGACCCGGA TCCGGGCATG 
GTCCTCTTCC TGTCCACCAT CGACAAGAGC AAGGAGTTCT ACACGACCAT CGTGGACAAC 
TTCTGCGATG AGCAACAGCA CTCTCGTGAC GATCACTCCT GCTGGTCAGG CGATCGTTTC 
GGGGACTACA CTCAGCTGCT GATTAATCCG GGCACTGATA GCCAGCGATA TAATCCCGAG 
GTGCCCTTCA ATGCGAAGGC TCAGACCGGC AAGCTGAACG AGCTGGTCGA CAAGCTCTTC 
AAGATTCGCA AGAGCATTGG AGCTGCGGCA CCTTCCAACA GCATACAGGC GACGCACGAC 
ATCCAGAATG ATATGGGTGA GGGCTCCGGC GGCGGAGAAG GTCAGATCGG CGACGATGAG 
GAGGAGTATG GAGGCGCACA TGGTTCCGGA GATGGTTCCG GCGATGGACC CCATACGCCC 
ATCGAGGAAT CCGAAGGCAC CACCACCAAC GAAGTGGAGT CGCGTGACAG CGGCAAGACC 
AGTGGGTCCA ACCCACTGGA AGGCACCGCG ACGTGGATGC TCCTCACTCT AGTCACAATG 
CTCTTTAGTA GCTGTAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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dally cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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