foi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	foi
Entrez Gene ID	38976
Full Name	fear-of-intimacy
Synonyms	CG6817,Dmel\CG6817,FOI,Foi,cg6817,dFOI,dZIPfoi,dZipFOI,kas,l(3)j8E8,l(3)neo13
General protein information	Preferred Names fear-of-intimacy Names CG6817 gene product from transcript CG6817-RD CG6817-PA CG6817-PB CG6817-PC CG6817-PD fear of intimacy foi-PA foi-PB foi-PC foi-PD kastchen
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66D5-66D6

mRNA and Protein(s)

mRNA	Protein	Name
NM_079250.2	NP_523974.3	fear-of-intimacy, isoform A
NM_001274656.1	NP_001261585.1	fear-of-intimacy, isoform C
NM_001274655.1	NP_001261584.1	fear-of-intimacy, isoform B
NM_001300056.1	NP_001286985.1	fear-of-intimacy, isoform D

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SLC39A10	XP_005640789.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005405	XP_315414.4
Drosophila melanogaster (fruit fly)	foi	NP_001261584.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ZIP transporter Fear-of-intimacy (Foi) is necessary for the formation of Drosophila muscles.
Title: Fear-of-intimacy-mediated zinc transport controls the function of zinc-finger transcription factors involved in myogenesis.

in vivo structure-function analysis of FOI that strongly indicates that zinc transport activity of FOI is essential for gonad development
Title: Zinc transport activity of Fear of Intimacy is essential for proper gonad morphogenesis and DE-cadherin expression.

cell-surface protein required in the mesoderm for gonad morphogenesis
Title: fear of intimacy encodes a novel transmembrane protein required for gonad morphogenesis in Drosophila.

The following foi gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the foi cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17895	NM_079250.2 Latest version!	Drosophila melanogaster fear-of-intimacy (foi), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa17895	NM_001274656.1 Latest version!	Drosophila melanogaster fear-of-intimacy (foi), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa17895	NM_001274655.1 Latest version!	Drosophila melanogaster fear-of-intimacy (foi), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa17895	NM_001300056.1 Latest version!	Drosophila melanogaster fear-of-intimacy (foi), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

foi ( NM_079250.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523974.3 Drosophila melanogaster fear-of-intimacy (foi), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17895
Clone ID Related Accession (Same CDS sequence)	NM_079250.2 , NM_001274656.1 , NM_001274655.1 , NM_001300056.1
Accession Version	NM_079250.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fear-of-intimacy, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Nov 6, 2002 this sequence version replaced NM_079250.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCGTC ACATAATGGC TGTATGCGTG GTGTGCCTAC TCTGTGCACA CCGTCTGCAC 
TGTCAGGATC ACATCGAGAG TCTGCTGGGA CCGGCGCGGG TCACGACCCA CAACAGCCAG 
GACCAGTTGA ATGCCCGCGT CTACACGAAC CTAAGTCCAT CCAGTGAAAC CACCGATCGG 
CGACAGCAGC GATCCGCTTC TGGCGACGAC GACACCTTCA ACTACAGCAT CAGCCCGCCA 
TCGAGAAGAG AAAAGCGCCA TGCTGGCCAT GAGCATGGCC CCACATCCGA ATCACGCGTC 
CCGCAGATCA CCCAGTACTA TCTCGAGAAG CTGATGGCCC AGGATGAGCT GATGAACAGC 
AGCGGATTCG ATGGCCTACT CCAGCAGCTC AGCCTCCACT CGTTGGCCAG TGGGGCAAGT 
GAGGGAACTT GCGTTCCTGG TAGTCGCCTT GTGCATCACG TCCAGCCCCA CGATCATCAC 
CATGCTCATC ATCACGAAGA AGAAGATCAC AGTCTGCAGC TAAACAACTG CACGCTTATC 
CAGAATGGCA CCACCTCCAA CGTCATATGC CCATCCCTGC CCAACAACAA CACACACCCA 
CTCGGCAAAG AGGCCAAGAA CTTTACGCTG AGCGATAAGG ACTTGCTACA TCTGTGTCCG 
ATTCTCCTAT ATGAGCTGAA AGCCCAGAGC GGCGGATGCA TAGAACCTGC TATCTTGTCC 
GACATTGATA CCACTGAAGA ACTGCTGGAA GCAGAGAAGG ACAAGGATAT ATTCTATGTA 
TGGATCTATG CTTTTATATC TGTGTTTGCC TGCGGCATTC TCGGCTTGGT AGGCGTGGCC 
ATCATACCGT TTATGGGTTC CAGGTATTAC AAGTACATCA TTCAATATCT GGTAGCGCTG 
GCCGTTGGTA CGATGACTGG CGATGCCCTA CTTCACTTGC TGCCTCATTC TCTTGCAGGC 
CAGGATGAGC GGGGGATGAT CATGAAAGGA CTGGGTTGCC TAGGTGGCAT CATATTCTTT 
TACGTGATGG AGCATGCGCT GACTATGATC TCTGAGTGGC GCAAGAGCGT AGAGAAGAAG 
GAGACAAAGA AACCATCGCG GGCAAAGGTG ATGCGGGATC CGGACTCGTC GGTAAACAAC 
TCCGTGGCCG GCGACAAGAT CTGCAAGCAA AAGTACAGCT CCTATCCATA TTGCTACGAC 
GAGATCACCA TGAACAACAA GCAGAGTGAG TGGATGCACC TTCCCTTCGA TGTAGCGGCG 
GGCGCTGGCG GAGATGCACC TTCAGTAGCG GAGCTACGTA ACGGTGTGGG CGATCATGAT 
GGATCCAATG ACATGGCCGC CGCTGCCGAG TCCCTAATAT CACCGCTGCA CACGAACTGC 
GTGGAAATGA ATCACCATAA TCACAACCAC AAGCACAATA GCCACCAGCA GAATCATGAG 
GGCCAGGATA GCAACACAAT TGTCACGGAT CTTGACGGAA ACGCCGTGTA CGCAGTAAAC 
AAGGCAAAGG ATAAGGACAG CCGGAACGAT CATGTGACTG TGATCCTGCG GGAGCACGAG 
TCCTCGCATC ACGGTCACAG TCATCGCCAT GGACACGTCC ATTCGCCGCC GGAAACGTTG 
AGCGCCGTGG CCTGGATGAT TATCATGGGT GACGGTCTGC ACAATTTTAC GGATGGCATG 
GCCATTGGTG CAGCCTTTGC GGAAAACATT GCCGGCGGCT TCTCCACATC GCTGGCTGTC 
TTCTGCCACG AATTGCCACA CGAGCTGGGT GACTTTGCCA TCTTAATAAA AGCGGGCATG 
TCGGTAAAGT CGGCCGTCTA CTACAACCTG TTGACTGGAG TCCTGAGTTT CATCGGCATG 
ATCTTTGGCA TTGCCTTTGG ACAATCGCAG GATGTGGCCC AGTGGATGTT TGCCGTGGCT 
GCGGGTCTGT TCATCTACAT TGCCCTAGTC GATATGATGC CAGAGATCTC GGCCTCGCAC 
AAATCACTGG GCCAGTTTCT GCTGCAGATT CTCGGCATGC TCAGCGGAGT GGGGATAATG 
CTATTGATTG CCCTTTACGA GGGCGATCTG ATGAGCGCGT TCGGCACAGC GGGAGCCGCA 
TCACACCAGC ACGCGCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523974.3
CDS	623..2743
Translation	MARHIMAVCVVCLLCAHRLHCQDHIESLLGPARVTTHNSQDQLNARVYTNLSPSSETTDRRQQRSASGDDDTFNYSISPPSRREKRHAGHEHGPTSESRVPQITQYYLEKLMAQDELMNSSGFDGLLQQLSLHSLASGASEGTCVPGSRLVHHVQPHDHHHAHHHEEEDHSLQLNNCTLIQNGTTSNVICPSLPNNNTHPLGKEAKNFTLSDKDLLHLCPILLYELKAQSGGCIEPAILSDIDTTEELLEAEKDKDIFYVWIYAFISVFACGILGLVGVAIIPFMGSRYYKYIIQYLVALAVGTMTGDALLHLLPHSLAGQDERGMIMKGLGCLGGIIFFYVMEHALTMISEWRKSVEKKETKKPSRAKVMRDPDSSVNNSVAGDKICKQKYSSYPYCYDEITMNNKQSEWMHLPFDVAAGAGGDAPSVAELRNGVGDHDGSNDMAAAAESLISPLHTNCVEMNHHNHNHKHNSHQQNHEGQDSNTIVTDLDGNAVYAVNKAKDKDSRNDHVTVILREHESSHHGHSHRHGHVHSPPETLSAVAWMIIMGDGLHNFTDGMAIGAAFAENIAGGFSTSLAVFCHELPHELGDFAILIKAGMSVKSAVYYNLLTGVLSFIGMIFGIAFGQSQDVAQWMFAVAAGLFIYIALVDMMPEISASHKSLGQFLLQILGMLSGVGIMLLIALYEGDLMSAFGTAGAASHQHAH

Target ORF information:

RefSeq Version	NM_079250.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fear-of-intimacy (foi), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079250.2

ORF Insert Sequence:

ATGGCGCGTC ACATAATGGC TGTATGCGTG GTGTGCCTAC TCTGTGCACA CCGTCTGCAC 
TGTCAGGATC ACATCGAGAG TCTGCTGGGA CCGGCGCGGG TCACGACCCA CAACAGCCAG 
GACCAGTTGA ATGCCCGCGT CTACACGAAC CTAAGTCCAT CCAGTGAAAC CACCGATCGG 
CGACAGCAGC GATCCGCTTC TGGCGACGAC GACACCTTCA ACTACAGCAT CAGCCCGCCA 
TCGAGAAGAG AAAAGCGCCA TGCTGGCCAT GAGCATGGCC CCACATCCGA ATCACGCGTC 
CCGCAGATCA CCCAGTACTA TCTCGAGAAG CTGATGGCCC AGGATGAGCT GATGAACAGC 
AGCGGATTCG ATGGCCTACT CCAGCAGCTC AGCCTCCACT CGTTGGCCAG TGGGGCAAGT 
GAGGGAACTT GCGTTCCTGG TAGTCGCCTT GTGCATCACG TCCAGCCCCA CGATCATCAC 
CATGCTCATC ATCACGAAGA AGAAGATCAC AGTCTGCAGC TAAACAACTG CACGCTTATC 
CAGAATGGCA CCACCTCCAA CGTCATATGC CCATCCCTGC CCAACAACAA CACACACCCA 
CTCGGCAAAG AGGCCAAGAA CTTTACGCTG AGCGATAAGG ACTTGCTACA TCTGTGTCCG 
ATTCTCCTAT ATGAGCTGAA AGCCCAGAGC GGCGGATGCA TAGAACCTGC TATCTTGTCC 
GACATTGATA CCACTGAAGA ACTGCTGGAA GCAGAGAAGG ACAAGGATAT ATTCTATGTA 
TGGATCTATG CTTTTATATC TGTGTTTGCC TGCGGCATTC TCGGCTTGGT AGGCGTGGCC 
ATCATACCGT TTATGGGTTC CAGGTATTAC AAGTACATCA TTCAATATCT GGTAGCGCTG 
GCCGTTGGTA CGATGACTGG CGATGCCCTA CTTCACTTGC TGCCTCATTC TCTTGCAGGC 
CAGGATGAGC GGGGGATGAT CATGAAAGGA CTGGGTTGCC TAGGTGGCAT CATATTCTTT 
TACGTGATGG AGCATGCGCT GACTATGATC TCTGAGTGGC GCAAGAGCGT AGAGAAGAAG 
GAGACAAAGA AACCATCGCG GGCAAAGGTG ATGCGGGATC CGGACTCGTC GGTAAACAAC 
TCCGTGGCCG GCGACAAGAT CTGCAAGCAA AAGTACAGCT CCTATCCATA TTGCTACGAC 
GAGATCACCA TGAACAACAA GCAGAGTGAG TGGATGCACC TTCCCTTCGA TGTAGCGGCG 
GGCGCTGGCG GAGATGCACC TTCAGTAGCG GAGCTACGTA ACGGTGTGGG CGATCATGAT 
GGATCCAATG ACATGGCCGC CGCTGCCGAG TCCCTAATAT CACCGCTGCA CACGAACTGC 
GTGGAAATGA ATCACCATAA TCACAACCAC AAGCACAATA GCCACCAGCA GAATCATGAG 
GGCCAGGATA GCAACACAAT TGTCACGGAT CTTGACGGAA ACGCCGTGTA CGCAGTAAAC 
AAGGCAAAGG ATAAGGACAG CCGGAACGAT CATGTGACTG TGATCCTGCG GGAGCACGAG 
TCCTCGCATC ACGGTCACAG TCATCGCCAT GGACACGTCC ATTCGCCGCC GGAAACGTTG 
AGCGCCGTGG CCTGGATGAT TATCATGGGT GACGGTCTGC ACAATTTTAC GGATGGCATG 
GCCATTGGTG CAGCCTTTGC GGAAAACATT GCCGGCGGCT TCTCCACATC GCTGGCTGTC 
TTCTGCCACG AATTGCCACA CGAGCTGGGT GACTTTGCCA TCTTAATAAA AGCGGGCATG 
TCGGTAAAGT CGGCCGTCTA CTACAACCTG TTGACTGGAG TCCTGAGTTT CATCGGCATG 
ATCTTTGGCA TTGCCTTTGG ACAATCGCAG GATGTGGCCC AGTGGATGTT TGCCGTGGCT 
GCGGGTCTGT TCATCTACAT TGCCCTAGTC GATATGATGC CAGAGATCTC GGCCTCGCAC 
AAATCACTGG GCCAGTTTCT GCTGCAGATT CTCGGCATGC TCAGCGGAGT GGGGATAATG 
CTATTGATTG CCCTTTACGA GGGCGATCTG ATGAGCGCGT TCGGCACAGC GGGAGCCGCA 
TCACACCAGC ACGCGCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

foi ( NM_001274656.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261585.1 Drosophila melanogaster fear-of-intimacy (foi), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa17895
Clone ID Related Accession (Same CDS sequence)	NM_079250.2 , NM_001274656.1 , NM_001274655.1 , NM_001300056.1
Accession Version	NM_001274656.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fear-of-intimacy, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCGTC ACATAATGGC TGTATGCGTG GTGTGCCTAC TCTGTGCACA CCGTCTGCAC 
TGTCAGGATC ACATCGAGAG TCTGCTGGGA CCGGCGCGGG TCACGACCCA CAACAGCCAG 
GACCAGTTGA ATGCCCGCGT CTACACGAAC CTAAGTCCAT CCAGTGAAAC CACCGATCGG 
CGACAGCAGC GATCCGCTTC TGGCGACGAC GACACCTTCA ACTACAGCAT CAGCCCGCCA 
TCGAGAAGAG AAAAGCGCCA TGCTGGCCAT GAGCATGGCC CCACATCCGA ATCACGCGTC 
CCGCAGATCA CCCAGTACTA TCTCGAGAAG CTGATGGCCC AGGATGAGCT GATGAACAGC 
AGCGGATTCG ATGGCCTACT CCAGCAGCTC AGCCTCCACT CGTTGGCCAG TGGGGCAAGT 
GAGGGAACTT GCGTTCCTGG TAGTCGCCTT GTGCATCACG TCCAGCCCCA CGATCATCAC 
CATGCTCATC ATCACGAAGA AGAAGATCAC AGTCTGCAGC TAAACAACTG CACGCTTATC 
CAGAATGGCA CCACCTCCAA CGTCATATGC CCATCCCTGC CCAACAACAA CACACACCCA 
CTCGGCAAAG AGGCCAAGAA CTTTACGCTG AGCGATAAGG ACTTGCTACA TCTGTGTCCG 
ATTCTCCTAT ATGAGCTGAA AGCCCAGAGC GGCGGATGCA TAGAACCTGC TATCTTGTCC 
GACATTGATA CCACTGAAGA ACTGCTGGAA GCAGAGAAGG ACAAGGATAT ATTCTATGTA 
TGGATCTATG CTTTTATATC TGTGTTTGCC TGCGGCATTC TCGGCTTGGT AGGCGTGGCC 
ATCATACCGT TTATGGGTTC CAGGTATTAC AAGTACATCA TTCAATATCT GGTAGCGCTG 
GCCGTTGGTA CGATGACTGG CGATGCCCTA CTTCACTTGC TGCCTCATTC TCTTGCAGGC 
CAGGATGAGC GGGGGATGAT CATGAAAGGA CTGGGTTGCC TAGGTGGCAT CATATTCTTT 
TACGTGATGG AGCATGCGCT GACTATGATC TCTGAGTGGC GCAAGAGCGT AGAGAAGAAG 
GAGACAAAGA AACCATCGCG GGCAAAGGTG ATGCGGGATC CGGACTCGTC GGTAAACAAC 
TCCGTGGCCG GCGACAAGAT CTGCAAGCAA AAGTACAGCT CCTATCCATA TTGCTACGAC 
GAGATCACCA TGAACAACAA GCAGAGTGAG TGGATGCACC TTCCCTTCGA TGTAGCGGCG 
GGCGCTGGCG GAGATGCACC TTCAGTAGCG GAGCTACGTA ACGGTGTGGG CGATCATGAT 
GGATCCAATG ACATGGCCGC CGCTGCCGAG TCCCTAATAT CACCGCTGCA CACGAACTGC 
GTGGAAATGA ATCACCATAA TCACAACCAC AAGCACAATA GCCACCAGCA GAATCATGAG 
GGCCAGGATA GCAACACAAT TGTCACGGAT CTTGACGGAA ACGCCGTGTA CGCAGTAAAC 
AAGGCAAAGG ATAAGGACAG CCGGAACGAT CATGTGACTG TGATCCTGCG GGAGCACGAG 
TCCTCGCATC ACGGTCACAG TCATCGCCAT GGACACGTCC ATTCGCCGCC GGAAACGTTG 
AGCGCCGTGG CCTGGATGAT TATCATGGGT GACGGTCTGC ACAATTTTAC GGATGGCATG 
GCCATTGGTG CAGCCTTTGC GGAAAACATT GCCGGCGGCT TCTCCACATC GCTGGCTGTC 
TTCTGCCACG AATTGCCACA CGAGCTGGGT GACTTTGCCA TCTTAATAAA AGCGGGCATG 
TCGGTAAAGT CGGCCGTCTA CTACAACCTG TTGACTGGAG TCCTGAGTTT CATCGGCATG 
ATCTTTGGCA TTGCCTTTGG ACAATCGCAG GATGTGGCCC AGTGGATGTT TGCCGTGGCT 
GCGGGTCTGT TCATCTACAT TGCCCTAGTC GATATGATGC CAGAGATCTC GGCCTCGCAC 
AAATCACTGG GCCAGTTTCT GCTGCAGATT CTCGGCATGC TCAGCGGAGT GGGGATAATG 
CTATTGATTG CCCTTTACGA GGGCGATCTG ATGAGCGCGT TCGGCACAGC GGGAGCCGCA 
TCACACCAGC ACGCGCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261585.1
CDS	308..2428
Translation	MARHIMAVCVVCLLCAHRLHCQDHIESLLGPARVTTHNSQDQLNARVYTNLSPSSETTDRRQQRSASGDDDTFNYSISPPSRREKRHAGHEHGPTSESRVPQITQYYLEKLMAQDELMNSSGFDGLLQQLSLHSLASGASEGTCVPGSRLVHHVQPHDHHHAHHHEEEDHSLQLNNCTLIQNGTTSNVICPSLPNNNTHPLGKEAKNFTLSDKDLLHLCPILLYELKAQSGGCIEPAILSDIDTTEELLEAEKDKDIFYVWIYAFISVFACGILGLVGVAIIPFMGSRYYKYIIQYLVALAVGTMTGDALLHLLPHSLAGQDERGMIMKGLGCLGGIIFFYVMEHALTMISEWRKSVEKKETKKPSRAKVMRDPDSSVNNSVAGDKICKQKYSSYPYCYDEITMNNKQSEWMHLPFDVAAGAGGDAPSVAELRNGVGDHDGSNDMAAAAESLISPLHTNCVEMNHHNHNHKHNSHQQNHEGQDSNTIVTDLDGNAVYAVNKAKDKDSRNDHVTVILREHESSHHGHSHRHGHVHSPPETLSAVAWMIIMGDGLHNFTDGMAIGAAFAENIAGGFSTSLAVFCHELPHELGDFAILIKAGMSVKSAVYYNLLTGVLSFIGMIFGIAFGQSQDVAQWMFAVAAGLFIYIALVDMMPEISASHKSLGQFLLQILGMLSGVGIMLLIALYEGDLMSAFGTAGAASHQHAH

Target ORF information:

RefSeq Version	NM_001274656.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fear-of-intimacy (foi), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274656.1

ORF Insert Sequence:

ATGGCGCGTC ACATAATGGC TGTATGCGTG GTGTGCCTAC TCTGTGCACA CCGTCTGCAC 
TGTCAGGATC ACATCGAGAG TCTGCTGGGA CCGGCGCGGG TCACGACCCA CAACAGCCAG 
GACCAGTTGA ATGCCCGCGT CTACACGAAC CTAAGTCCAT CCAGTGAAAC CACCGATCGG 
CGACAGCAGC GATCCGCTTC TGGCGACGAC GACACCTTCA ACTACAGCAT CAGCCCGCCA 
TCGAGAAGAG AAAAGCGCCA TGCTGGCCAT GAGCATGGCC CCACATCCGA ATCACGCGTC 
CCGCAGATCA CCCAGTACTA TCTCGAGAAG CTGATGGCCC AGGATGAGCT GATGAACAGC 
AGCGGATTCG ATGGCCTACT CCAGCAGCTC AGCCTCCACT CGTTGGCCAG TGGGGCAAGT 
GAGGGAACTT GCGTTCCTGG TAGTCGCCTT GTGCATCACG TCCAGCCCCA CGATCATCAC 
CATGCTCATC ATCACGAAGA AGAAGATCAC AGTCTGCAGC TAAACAACTG CACGCTTATC 
CAGAATGGCA CCACCTCCAA CGTCATATGC CCATCCCTGC CCAACAACAA CACACACCCA 
CTCGGCAAAG AGGCCAAGAA CTTTACGCTG AGCGATAAGG ACTTGCTACA TCTGTGTCCG 
ATTCTCCTAT ATGAGCTGAA AGCCCAGAGC GGCGGATGCA TAGAACCTGC TATCTTGTCC 
GACATTGATA CCACTGAAGA ACTGCTGGAA GCAGAGAAGG ACAAGGATAT ATTCTATGTA 
TGGATCTATG CTTTTATATC TGTGTTTGCC TGCGGCATTC TCGGCTTGGT AGGCGTGGCC 
ATCATACCGT TTATGGGTTC CAGGTATTAC AAGTACATCA TTCAATATCT GGTAGCGCTG 
GCCGTTGGTA CGATGACTGG CGATGCCCTA CTTCACTTGC TGCCTCATTC TCTTGCAGGC 
CAGGATGAGC GGGGGATGAT CATGAAAGGA CTGGGTTGCC TAGGTGGCAT CATATTCTTT 
TACGTGATGG AGCATGCGCT GACTATGATC TCTGAGTGGC GCAAGAGCGT AGAGAAGAAG 
GAGACAAAGA AACCATCGCG GGCAAAGGTG ATGCGGGATC CGGACTCGTC GGTAAACAAC 
TCCGTGGCCG GCGACAAGAT CTGCAAGCAA AAGTACAGCT CCTATCCATA TTGCTACGAC 
GAGATCACCA TGAACAACAA GCAGAGTGAG TGGATGCACC TTCCCTTCGA TGTAGCGGCG 
GGCGCTGGCG GAGATGCACC TTCAGTAGCG GAGCTACGTA ACGGTGTGGG CGATCATGAT 
GGATCCAATG ACATGGCCGC CGCTGCCGAG TCCCTAATAT CACCGCTGCA CACGAACTGC 
GTGGAAATGA ATCACCATAA TCACAACCAC AAGCACAATA GCCACCAGCA GAATCATGAG 
GGCCAGGATA GCAACACAAT TGTCACGGAT CTTGACGGAA ACGCCGTGTA CGCAGTAAAC 
AAGGCAAAGG ATAAGGACAG CCGGAACGAT CATGTGACTG TGATCCTGCG GGAGCACGAG 
TCCTCGCATC ACGGTCACAG TCATCGCCAT GGACACGTCC ATTCGCCGCC GGAAACGTTG 
AGCGCCGTGG CCTGGATGAT TATCATGGGT GACGGTCTGC ACAATTTTAC GGATGGCATG 
GCCATTGGTG CAGCCTTTGC GGAAAACATT GCCGGCGGCT TCTCCACATC GCTGGCTGTC 
TTCTGCCACG AATTGCCACA CGAGCTGGGT GACTTTGCCA TCTTAATAAA AGCGGGCATG 
TCGGTAAAGT CGGCCGTCTA CTACAACCTG TTGACTGGAG TCCTGAGTTT CATCGGCATG 
ATCTTTGGCA TTGCCTTTGG ACAATCGCAG GATGTGGCCC AGTGGATGTT TGCCGTGGCT 
GCGGGTCTGT TCATCTACAT TGCCCTAGTC GATATGATGC CAGAGATCTC GGCCTCGCAC 
AAATCACTGG GCCAGTTTCT GCTGCAGATT CTCGGCATGC TCAGCGGAGT GGGGATAATG 
CTATTGATTG CCCTTTACGA GGGCGATCTG ATGAGCGCGT TCGGCACAGC GGGAGCCGCA 
TCACACCAGC ACGCGCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

foi ( NM_001274655.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261584.1 Drosophila melanogaster fear-of-intimacy (foi), transcript variant B, mRNA.

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CloneID	OFa17895
Clone ID Related Accession (Same CDS sequence)	NM_079250.2 , NM_001274656.1 , NM_001274655.1 , NM_001300056.1
Accession Version	NM_001274655.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fear-of-intimacy, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCGTC ACATAATGGC TGTATGCGTG GTGTGCCTAC TCTGTGCACA CCGTCTGCAC 
TGTCAGGATC ACATCGAGAG TCTGCTGGGA CCGGCGCGGG TCACGACCCA CAACAGCCAG 
GACCAGTTGA ATGCCCGCGT CTACACGAAC CTAAGTCCAT CCAGTGAAAC CACCGATCGG 
CGACAGCAGC GATCCGCTTC TGGCGACGAC GACACCTTCA ACTACAGCAT CAGCCCGCCA 
TCGAGAAGAG AAAAGCGCCA TGCTGGCCAT GAGCATGGCC CCACATCCGA ATCACGCGTC 
CCGCAGATCA CCCAGTACTA TCTCGAGAAG CTGATGGCCC AGGATGAGCT GATGAACAGC 
AGCGGATTCG ATGGCCTACT CCAGCAGCTC AGCCTCCACT CGTTGGCCAG TGGGGCAAGT 
GAGGGAACTT GCGTTCCTGG TAGTCGCCTT GTGCATCACG TCCAGCCCCA CGATCATCAC 
CATGCTCATC ATCACGAAGA AGAAGATCAC AGTCTGCAGC TAAACAACTG CACGCTTATC 
CAGAATGGCA CCACCTCCAA CGTCATATGC CCATCCCTGC CCAACAACAA CACACACCCA 
CTCGGCAAAG AGGCCAAGAA CTTTACGCTG AGCGATAAGG ACTTGCTACA TCTGTGTCCG 
ATTCTCCTAT ATGAGCTGAA AGCCCAGAGC GGCGGATGCA TAGAACCTGC TATCTTGTCC 
GACATTGATA CCACTGAAGA ACTGCTGGAA GCAGAGAAGG ACAAGGATAT ATTCTATGTA 
TGGATCTATG CTTTTATATC TGTGTTTGCC TGCGGCATTC TCGGCTTGGT AGGCGTGGCC 
ATCATACCGT TTATGGGTTC CAGGTATTAC AAGTACATCA TTCAATATCT GGTAGCGCTG 
GCCGTTGGTA CGATGACTGG CGATGCCCTA CTTCACTTGC TGCCTCATTC TCTTGCAGGC 
CAGGATGAGC GGGGGATGAT CATGAAAGGA CTGGGTTGCC TAGGTGGCAT CATATTCTTT 
TACGTGATGG AGCATGCGCT GACTATGATC TCTGAGTGGC GCAAGAGCGT AGAGAAGAAG 
GAGACAAAGA AACCATCGCG GGCAAAGGTG ATGCGGGATC CGGACTCGTC GGTAAACAAC 
TCCGTGGCCG GCGACAAGAT CTGCAAGCAA AAGTACAGCT CCTATCCATA TTGCTACGAC 
GAGATCACCA TGAACAACAA GCAGAGTGAG TGGATGCACC TTCCCTTCGA TGTAGCGGCG 
GGCGCTGGCG GAGATGCACC TTCAGTAGCG GAGCTACGTA ACGGTGTGGG CGATCATGAT 
GGATCCAATG ACATGGCCGC CGCTGCCGAG TCCCTAATAT CACCGCTGCA CACGAACTGC 
GTGGAAATGA ATCACCATAA TCACAACCAC AAGCACAATA GCCACCAGCA GAATCATGAG 
GGCCAGGATA GCAACACAAT TGTCACGGAT CTTGACGGAA ACGCCGTGTA CGCAGTAAAC 
AAGGCAAAGG ATAAGGACAG CCGGAACGAT CATGTGACTG TGATCCTGCG GGAGCACGAG 
TCCTCGCATC ACGGTCACAG TCATCGCCAT GGACACGTCC ATTCGCCGCC GGAAACGTTG 
AGCGCCGTGG CCTGGATGAT TATCATGGGT GACGGTCTGC ACAATTTTAC GGATGGCATG 
GCCATTGGTG CAGCCTTTGC GGAAAACATT GCCGGCGGCT TCTCCACATC GCTGGCTGTC 
TTCTGCCACG AATTGCCACA CGAGCTGGGT GACTTTGCCA TCTTAATAAA AGCGGGCATG 
TCGGTAAAGT CGGCCGTCTA CTACAACCTG TTGACTGGAG TCCTGAGTTT CATCGGCATG 
ATCTTTGGCA TTGCCTTTGG ACAATCGCAG GATGTGGCCC AGTGGATGTT TGCCGTGGCT 
GCGGGTCTGT TCATCTACAT TGCCCTAGTC GATATGATGC CAGAGATCTC GGCCTCGCAC 
AAATCACTGG GCCAGTTTCT GCTGCAGATT CTCGGCATGC TCAGCGGAGT GGGGATAATG 
CTATTGATTG CCCTTTACGA GGGCGATCTG ATGAGCGCGT TCGGCACAGC GGGAGCCGCA 
TCACACCAGC ACGCGCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261584.1
CDS	231..2351
Translation	MARHIMAVCVVCLLCAHRLHCQDHIESLLGPARVTTHNSQDQLNARVYTNLSPSSETTDRRQQRSASGDDDTFNYSISPPSRREKRHAGHEHGPTSESRVPQITQYYLEKLMAQDELMNSSGFDGLLQQLSLHSLASGASEGTCVPGSRLVHHVQPHDHHHAHHHEEEDHSLQLNNCTLIQNGTTSNVICPSLPNNNTHPLGKEAKNFTLSDKDLLHLCPILLYELKAQSGGCIEPAILSDIDTTEELLEAEKDKDIFYVWIYAFISVFACGILGLVGVAIIPFMGSRYYKYIIQYLVALAVGTMTGDALLHLLPHSLAGQDERGMIMKGLGCLGGIIFFYVMEHALTMISEWRKSVEKKETKKPSRAKVMRDPDSSVNNSVAGDKICKQKYSSYPYCYDEITMNNKQSEWMHLPFDVAAGAGGDAPSVAELRNGVGDHDGSNDMAAAAESLISPLHTNCVEMNHHNHNHKHNSHQQNHEGQDSNTIVTDLDGNAVYAVNKAKDKDSRNDHVTVILREHESSHHGHSHRHGHVHSPPETLSAVAWMIIMGDGLHNFTDGMAIGAAFAENIAGGFSTSLAVFCHELPHELGDFAILIKAGMSVKSAVYYNLLTGVLSFIGMIFGIAFGQSQDVAQWMFAVAAGLFIYIALVDMMPEISASHKSLGQFLLQILGMLSGVGIMLLIALYEGDLMSAFGTAGAASHQHAH

Target ORF information:

RefSeq Version	NM_001274655.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fear-of-intimacy (foi), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274655.1

ORF Insert Sequence:

ATGGCGCGTC ACATAATGGC TGTATGCGTG GTGTGCCTAC TCTGTGCACA CCGTCTGCAC 
TGTCAGGATC ACATCGAGAG TCTGCTGGGA CCGGCGCGGG TCACGACCCA CAACAGCCAG 
GACCAGTTGA ATGCCCGCGT CTACACGAAC CTAAGTCCAT CCAGTGAAAC CACCGATCGG 
CGACAGCAGC GATCCGCTTC TGGCGACGAC GACACCTTCA ACTACAGCAT CAGCCCGCCA 
TCGAGAAGAG AAAAGCGCCA TGCTGGCCAT GAGCATGGCC CCACATCCGA ATCACGCGTC 
CCGCAGATCA CCCAGTACTA TCTCGAGAAG CTGATGGCCC AGGATGAGCT GATGAACAGC 
AGCGGATTCG ATGGCCTACT CCAGCAGCTC AGCCTCCACT CGTTGGCCAG TGGGGCAAGT 
GAGGGAACTT GCGTTCCTGG TAGTCGCCTT GTGCATCACG TCCAGCCCCA CGATCATCAC 
CATGCTCATC ATCACGAAGA AGAAGATCAC AGTCTGCAGC TAAACAACTG CACGCTTATC 
CAGAATGGCA CCACCTCCAA CGTCATATGC CCATCCCTGC CCAACAACAA CACACACCCA 
CTCGGCAAAG AGGCCAAGAA CTTTACGCTG AGCGATAAGG ACTTGCTACA TCTGTGTCCG 
ATTCTCCTAT ATGAGCTGAA AGCCCAGAGC GGCGGATGCA TAGAACCTGC TATCTTGTCC 
GACATTGATA CCACTGAAGA ACTGCTGGAA GCAGAGAAGG ACAAGGATAT ATTCTATGTA 
TGGATCTATG CTTTTATATC TGTGTTTGCC TGCGGCATTC TCGGCTTGGT AGGCGTGGCC 
ATCATACCGT TTATGGGTTC CAGGTATTAC AAGTACATCA TTCAATATCT GGTAGCGCTG 
GCCGTTGGTA CGATGACTGG CGATGCCCTA CTTCACTTGC TGCCTCATTC TCTTGCAGGC 
CAGGATGAGC GGGGGATGAT CATGAAAGGA CTGGGTTGCC TAGGTGGCAT CATATTCTTT 
TACGTGATGG AGCATGCGCT GACTATGATC TCTGAGTGGC GCAAGAGCGT AGAGAAGAAG 
GAGACAAAGA AACCATCGCG GGCAAAGGTG ATGCGGGATC CGGACTCGTC GGTAAACAAC 
TCCGTGGCCG GCGACAAGAT CTGCAAGCAA AAGTACAGCT CCTATCCATA TTGCTACGAC 
GAGATCACCA TGAACAACAA GCAGAGTGAG TGGATGCACC TTCCCTTCGA TGTAGCGGCG 
GGCGCTGGCG GAGATGCACC TTCAGTAGCG GAGCTACGTA ACGGTGTGGG CGATCATGAT 
GGATCCAATG ACATGGCCGC CGCTGCCGAG TCCCTAATAT CACCGCTGCA CACGAACTGC 
GTGGAAATGA ATCACCATAA TCACAACCAC AAGCACAATA GCCACCAGCA GAATCATGAG 
GGCCAGGATA GCAACACAAT TGTCACGGAT CTTGACGGAA ACGCCGTGTA CGCAGTAAAC 
AAGGCAAAGG ATAAGGACAG CCGGAACGAT CATGTGACTG TGATCCTGCG GGAGCACGAG 
TCCTCGCATC ACGGTCACAG TCATCGCCAT GGACACGTCC ATTCGCCGCC GGAAACGTTG 
AGCGCCGTGG CCTGGATGAT TATCATGGGT GACGGTCTGC ACAATTTTAC GGATGGCATG 
GCCATTGGTG CAGCCTTTGC GGAAAACATT GCCGGCGGCT TCTCCACATC GCTGGCTGTC 
TTCTGCCACG AATTGCCACA CGAGCTGGGT GACTTTGCCA TCTTAATAAA AGCGGGCATG 
TCGGTAAAGT CGGCCGTCTA CTACAACCTG TTGACTGGAG TCCTGAGTTT CATCGGCATG 
ATCTTTGGCA TTGCCTTTGG ACAATCGCAG GATGTGGCCC AGTGGATGTT TGCCGTGGCT 
GCGGGTCTGT TCATCTACAT TGCCCTAGTC GATATGATGC CAGAGATCTC GGCCTCGCAC 
AAATCACTGG GCCAGTTTCT GCTGCAGATT CTCGGCATGC TCAGCGGAGT GGGGATAATG 
CTATTGATTG CCCTTTACGA GGGCGATCTG ATGAGCGCGT TCGGCACAGC GGGAGCCGCA 
TCACACCAGC ACGCGCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

foi ( NM_001300056.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286985.1 Drosophila melanogaster fear-of-intimacy (foi), transcript variant D, mRNA.

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CloneID	OFa17895
Clone ID Related Accession (Same CDS sequence)	NM_079250.2 , NM_001274656.1 , NM_001274655.1 , NM_001300056.1
Accession Version	NM_001300056.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fear-of-intimacy, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCGTC ACATAATGGC TGTATGCGTG GTGTGCCTAC TCTGTGCACA CCGTCTGCAC 
TGTCAGGATC ACATCGAGAG TCTGCTGGGA CCGGCGCGGG TCACGACCCA CAACAGCCAG 
GACCAGTTGA ATGCCCGCGT CTACACGAAC CTAAGTCCAT CCAGTGAAAC CACCGATCGG 
CGACAGCAGC GATCCGCTTC TGGCGACGAC GACACCTTCA ACTACAGCAT CAGCCCGCCA 
TCGAGAAGAG AAAAGCGCCA TGCTGGCCAT GAGCATGGCC CCACATCCGA ATCACGCGTC 
CCGCAGATCA CCCAGTACTA TCTCGAGAAG CTGATGGCCC AGGATGAGCT GATGAACAGC 
AGCGGATTCG ATGGCCTACT CCAGCAGCTC AGCCTCCACT CGTTGGCCAG TGGGGCAAGT 
GAGGGAACTT GCGTTCCTGG TAGTCGCCTT GTGCATCACG TCCAGCCCCA CGATCATCAC 
CATGCTCATC ATCACGAAGA AGAAGATCAC AGTCTGCAGC TAAACAACTG CACGCTTATC 
CAGAATGGCA CCACCTCCAA CGTCATATGC CCATCCCTGC CCAACAACAA CACACACCCA 
CTCGGCAAAG AGGCCAAGAA CTTTACGCTG AGCGATAAGG ACTTGCTACA TCTGTGTCCG 
ATTCTCCTAT ATGAGCTGAA AGCCCAGAGC GGCGGATGCA TAGAACCTGC TATCTTGTCC 
GACATTGATA CCACTGAAGA ACTGCTGGAA GCAGAGAAGG ACAAGGATAT ATTCTATGTA 
TGGATCTATG CTTTTATATC TGTGTTTGCC TGCGGCATTC TCGGCTTGGT AGGCGTGGCC 
ATCATACCGT TTATGGGTTC CAGGTATTAC AAGTACATCA TTCAATATCT GGTAGCGCTG 
GCCGTTGGTA CGATGACTGG CGATGCCCTA CTTCACTTGC TGCCTCATTC TCTTGCAGGC 
CAGGATGAGC GGGGGATGAT CATGAAAGGA CTGGGTTGCC TAGGTGGCAT CATATTCTTT 
TACGTGATGG AGCATGCGCT GACTATGATC TCTGAGTGGC GCAAGAGCGT AGAGAAGAAG 
GAGACAAAGA AACCATCGCG GGCAAAGGTG ATGCGGGATC CGGACTCGTC GGTAAACAAC 
TCCGTGGCCG GCGACAAGAT CTGCAAGCAA AAGTACAGCT CCTATCCATA TTGCTACGAC 
GAGATCACCA TGAACAACAA GCAGAGTGAG TGGATGCACC TTCCCTTCGA TGTAGCGGCG 
GGCGCTGGCG GAGATGCACC TTCAGTAGCG GAGCTACGTA ACGGTGTGGG CGATCATGAT 
GGATCCAATG ACATGGCCGC CGCTGCCGAG TCCCTAATAT CACCGCTGCA CACGAACTGC 
GTGGAAATGA ATCACCATAA TCACAACCAC AAGCACAATA GCCACCAGCA GAATCATGAG 
GGCCAGGATA GCAACACAAT TGTCACGGAT CTTGACGGAA ACGCCGTGTA CGCAGTAAAC 
AAGGCAAAGG ATAAGGACAG CCGGAACGAT CATGTGACTG TGATCCTGCG GGAGCACGAG 
TCCTCGCATC ACGGTCACAG TCATCGCCAT GGACACGTCC ATTCGCCGCC GGAAACGTTG 
AGCGCCGTGG CCTGGATGAT TATCATGGGT GACGGTCTGC ACAATTTTAC GGATGGCATG 
GCCATTGGTG CAGCCTTTGC GGAAAACATT GCCGGCGGCT TCTCCACATC GCTGGCTGTC 
TTCTGCCACG AATTGCCACA CGAGCTGGGT GACTTTGCCA TCTTAATAAA AGCGGGCATG 
TCGGTAAAGT CGGCCGTCTA CTACAACCTG TTGACTGGAG TCCTGAGTTT CATCGGCATG 
ATCTTTGGCA TTGCCTTTGG ACAATCGCAG GATGTGGCCC AGTGGATGTT TGCCGTGGCT 
GCGGGTCTGT TCATCTACAT TGCCCTAGTC GATATGATGC CAGAGATCTC GGCCTCGCAC 
AAATCACTGG GCCAGTTTCT GCTGCAGATT CTCGGCATGC TCAGCGGAGT GGGGATAATG 
CTATTGATTG CCCTTTACGA GGGCGATCTG ATGAGCGCGT TCGGCACAGC GGGAGCCGCA 
TCACACCAGC ACGCGCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286985.1
CDS	623..2743
Translation	MARHIMAVCVVCLLCAHRLHCQDHIESLLGPARVTTHNSQDQLNARVYTNLSPSSETTDRRQQRSASGDDDTFNYSISPPSRREKRHAGHEHGPTSESRVPQITQYYLEKLMAQDELMNSSGFDGLLQQLSLHSLASGASEGTCVPGSRLVHHVQPHDHHHAHHHEEEDHSLQLNNCTLIQNGTTSNVICPSLPNNNTHPLGKEAKNFTLSDKDLLHLCPILLYELKAQSGGCIEPAILSDIDTTEELLEAEKDKDIFYVWIYAFISVFACGILGLVGVAIIPFMGSRYYKYIIQYLVALAVGTMTGDALLHLLPHSLAGQDERGMIMKGLGCLGGIIFFYVMEHALTMISEWRKSVEKKETKKPSRAKVMRDPDSSVNNSVAGDKICKQKYSSYPYCYDEITMNNKQSEWMHLPFDVAAGAGGDAPSVAELRNGVGDHDGSNDMAAAAESLISPLHTNCVEMNHHNHNHKHNSHQQNHEGQDSNTIVTDLDGNAVYAVNKAKDKDSRNDHVTVILREHESSHHGHSHRHGHVHSPPETLSAVAWMIIMGDGLHNFTDGMAIGAAFAENIAGGFSTSLAVFCHELPHELGDFAILIKAGMSVKSAVYYNLLTGVLSFIGMIFGIAFGQSQDVAQWMFAVAAGLFIYIALVDMMPEISASHKSLGQFLLQILGMLSGVGIMLLIALYEGDLMSAFGTAGAASHQHAH

Target ORF information:

RefSeq Version	NM_001300056.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fear-of-intimacy (foi), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300056.1

ORF Insert Sequence:

ATGGCGCGTC ACATAATGGC TGTATGCGTG GTGTGCCTAC TCTGTGCACA CCGTCTGCAC 
TGTCAGGATC ACATCGAGAG TCTGCTGGGA CCGGCGCGGG TCACGACCCA CAACAGCCAG 
GACCAGTTGA ATGCCCGCGT CTACACGAAC CTAAGTCCAT CCAGTGAAAC CACCGATCGG 
CGACAGCAGC GATCCGCTTC TGGCGACGAC GACACCTTCA ACTACAGCAT CAGCCCGCCA 
TCGAGAAGAG AAAAGCGCCA TGCTGGCCAT GAGCATGGCC CCACATCCGA ATCACGCGTC 
CCGCAGATCA CCCAGTACTA TCTCGAGAAG CTGATGGCCC AGGATGAGCT GATGAACAGC 
AGCGGATTCG ATGGCCTACT CCAGCAGCTC AGCCTCCACT CGTTGGCCAG TGGGGCAAGT 
GAGGGAACTT GCGTTCCTGG TAGTCGCCTT GTGCATCACG TCCAGCCCCA CGATCATCAC 
CATGCTCATC ATCACGAAGA AGAAGATCAC AGTCTGCAGC TAAACAACTG CACGCTTATC 
CAGAATGGCA CCACCTCCAA CGTCATATGC CCATCCCTGC CCAACAACAA CACACACCCA 
CTCGGCAAAG AGGCCAAGAA CTTTACGCTG AGCGATAAGG ACTTGCTACA TCTGTGTCCG 
ATTCTCCTAT ATGAGCTGAA AGCCCAGAGC GGCGGATGCA TAGAACCTGC TATCTTGTCC 
GACATTGATA CCACTGAAGA ACTGCTGGAA GCAGAGAAGG ACAAGGATAT ATTCTATGTA 
TGGATCTATG CTTTTATATC TGTGTTTGCC TGCGGCATTC TCGGCTTGGT AGGCGTGGCC 
ATCATACCGT TTATGGGTTC CAGGTATTAC AAGTACATCA TTCAATATCT GGTAGCGCTG 
GCCGTTGGTA CGATGACTGG CGATGCCCTA CTTCACTTGC TGCCTCATTC TCTTGCAGGC 
CAGGATGAGC GGGGGATGAT CATGAAAGGA CTGGGTTGCC TAGGTGGCAT CATATTCTTT 
TACGTGATGG AGCATGCGCT GACTATGATC TCTGAGTGGC GCAAGAGCGT AGAGAAGAAG 
GAGACAAAGA AACCATCGCG GGCAAAGGTG ATGCGGGATC CGGACTCGTC GGTAAACAAC 
TCCGTGGCCG GCGACAAGAT CTGCAAGCAA AAGTACAGCT CCTATCCATA TTGCTACGAC 
GAGATCACCA TGAACAACAA GCAGAGTGAG TGGATGCACC TTCCCTTCGA TGTAGCGGCG 
GGCGCTGGCG GAGATGCACC TTCAGTAGCG GAGCTACGTA ACGGTGTGGG CGATCATGAT 
GGATCCAATG ACATGGCCGC CGCTGCCGAG TCCCTAATAT CACCGCTGCA CACGAACTGC 
GTGGAAATGA ATCACCATAA TCACAACCAC AAGCACAATA GCCACCAGCA GAATCATGAG 
GGCCAGGATA GCAACACAAT TGTCACGGAT CTTGACGGAA ACGCCGTGTA CGCAGTAAAC 
AAGGCAAAGG ATAAGGACAG CCGGAACGAT CATGTGACTG TGATCCTGCG GGAGCACGAG 
TCCTCGCATC ACGGTCACAG TCATCGCCAT GGACACGTCC ATTCGCCGCC GGAAACGTTG 
AGCGCCGTGG CCTGGATGAT TATCATGGGT GACGGTCTGC ACAATTTTAC GGATGGCATG 
GCCATTGGTG CAGCCTTTGC GGAAAACATT GCCGGCGGCT TCTCCACATC GCTGGCTGTC 
TTCTGCCACG AATTGCCACA CGAGCTGGGT GACTTTGCCA TCTTAATAAA AGCGGGCATG 
TCGGTAAAGT CGGCCGTCTA CTACAACCTG TTGACTGGAG TCCTGAGTTT CATCGGCATG 
ATCTTTGGCA TTGCCTTTGG ACAATCGCAG GATGTGGCCC AGTGGATGTT TGCCGTGGCT 
GCGGGTCTGT TCATCTACAT TGCCCTAGTC GATATGATGC CAGAGATCTC GGCCTCGCAC 
AAATCACTGG GCCAGTTTCT GCTGCAGATT CTCGGCATGC TCAGCGGAGT GGGGATAATG 
CTATTGATTG CCCTTTACGA GGGCGATCTG ATGAGCGCGT TCGGCACAGC GGGAGCCGCA 
TCACACCAGC ACGCGCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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foi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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