Unr cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Unr
Entrez Gene ID	38963
Full Name	Upstream of N-ras
Synonyms	BcDNA:LD13080,CG7015,CR32028,Dmel\CG7015,Dmel_CR32028,MRE30,UNR,dUNR,unr
General protein information	Preferred Names Upstream of N-ras Names CG7015 gene product from transcript CG7015-RC CG7015-PA CG7015-PB CG7015-PC CG7015-PD CG7015-PE Unr-PA Unr-PB Unr-PC Unr-PD Unr-PE upstream of N-ras
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66C12-66C13

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274650.1	NP_001261579.1	upstream of N-ras, isoform D
NM_001300054.1	NP_001286983.1	upstream of N-ras, isoform E
NM_001259740.2	NP_001246669.1	upstream of N-ras, isoform C
NM_001144440.2	NP_001137912.1	upstream of N-ras, isoform B
NM_139969.4	NP_648226.2	upstream of N-ras, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	CSDE1	NP_001229820.1
Canis lupus familiaris (dog)	CSDE1	XP_533016.1
Bos taurus (cattle)	CSDE1	NP_001091494.1
Rattus norvegicus (Norway rat)	Csde1	NP_446458.1
Rattus norvegicus (Norway rat)	LOC100364335	XP_003751901.1
Gallus gallus (chicken)	CSDE1	NP_001026089.1
Danio rerio (zebrafish)	si:ch211-261n11.2	NP_001186365.1
Drosophila melanogaster (fruit fly)	Unr	NP_001137912.1
	CSDE1	XP_001111655.1
Danio rerio (zebrafish)	csde1	XP_005167014.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004937	XP_315026.4
Pan troglodytes (chimpanzee)	CSDE1	XP_003949558.1
Mus musculus (house mouse)	Csde1	NP_659150.1
Xenopus tropicalis (tropical clawed frog)	csde1	NP_001025700.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

2.8 A crystal structure, NMR and small-angle X-ray and neutron scattering data of the ternary Sxl-Unr-msl2 ribonucleoprotein complex featuring unprecedented intertwined interactions of two Sxl RNA recognition motifs, a Unr cold-shock domain and RNA
Title: Structural basis for the assembly of the Sxl-Unr translation regulatory complex.

UNR promotes the targeting of the activating male-specific-lethal complex to the X-chromosome by facilitating the interaction of two crucial subunits: the RNA helicase MLE and the long non-coding RNA roX2.
Title: UNR facilitates the interaction of MLE with the lncRNA roX2 during Drosophila dosage compensation.

These results suggest that UNR exploits the transcript diversity generated by alternative processing and alternative promoter usage to bind and regulate target genes in a sex-specific manner.
Title: Widespread generation of alternative UTRs contributes to sex-specific RNA binding by UNR.

Efficient msl-2 mRNA silencing via the 3' UTR requires both a poly(A) tail and PABP function, indicating that UNR directly interacts with PABP.
Title: The SXL-UNR corepressor complex uses a PABP-mediated mechanism to inhibit ribosome recruitment to msl-2 mRNA.

These results uncover dual sex-specific functions of UNR in dosage compensation.
Title: Dual sex-specific functions of Drosophila Upstream of N-ras in the control of X chromosome dosage compensation.

The following Unr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Unr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17890	NM_001259740.2 Latest version!	Drosophila melanogaster upstream of N-ras, transcript variant C (Unr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa17891	NM_001144440.2 Latest version!	Drosophila melanogaster upstream of N-ras, transcript variant B (Unr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa17891	NM_001274650.1 Latest version!	Drosophila melanogaster upstream of N-ras, transcript variant D (Unr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa17891	NM_001300054.1 Latest version!	Drosophila melanogaster upstream of N-ras, transcript variant E (Unr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa20734	NM_139969.4 Latest version!	Drosophila melanogaster upstream of N-ras, transcript variant A (Unr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Unr ( NM_001259740.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246669.1 Drosophila melanogaster upstream of N-ras, transcript variant C (Unr), mRNA.

Price & Availability↑

CloneID	OFa17890
Clone ID Related Accession (Same CDS sequence)	NM_001259740.2
Accession Version	NM_001259740.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	upstream of N-ras, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001259740.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATACCC AATCGAAGGT GTACCGTACC GGCGAAGAAA TCTATGACAA CTTGCCATGC 
GATAGTTATT TCAACATGAA TGCCATTCGC AACCTGGGAA TCCCAACAAC GTTTCCGACC 
ATTGGAACAT TTACACTTGA CTCTACTACG TTGGGACTGC AGCCGCAGGG TCAGGGCCCG 
TCGCCCCAGC AGCAGCAGCA TCAACAGCAA CAACAACAGC AGCAGCAGCA ACATCAGCAG 
AATATGCACC ACCATCAACA TCAGCAGCAA CACATGCAGC AGCAGCAGCA ACAACAACAA 
CATCAGCAGC AACACCAACA GCAGCAACAT CAACAACAAC AGCAACATCA GCAGCAGCAG 
CAGCAGCAAC AACAGCATCC AACCATTGGA ATGTTCGATG CCAACGAGGT TAACGATGTC 
ATCCAGAATC CTCCTCAAAT CGGGGTATTC CAGTCTAACA GCGTTCTGAC CAACGGTGCC 
GGCAGTGGCT CCTCGATCTT TGGCTCCCAA TCGAGCAATT CATCGGCGGC AGCTGCAGAT 
CCCAGCCAGA CCACCCGGGA AACTGGCATC ATTGAAAAGT TGTTGCATTC GTATGGATTC 
ATTCAGTGCT GTGAGCGGCA GGCGCGTCTT TTCTTTCATT TCTCGCAATT CAGCGGTAAT 
ATTGATCATT TGAAAATCGG AGATCCCGTG GAGTTTGAGA TGACCTATGA TCGCCGCACC 
GGCAAGCCGA TAGCTAGTCA AGTATCAAAA ATAGCCCCAG AGGTTGTTCT CTCCGAGGAG 
CGGGTCACCG GCACGGTGAC CACCGAGCTG CGCACCGATA GCGCCAACAA TGTGCTTAAC 
TCCAGCGAGA CCACAGGTCG CATAAGCTAT GAGAACCGAG GCGAATGCTT CTTTTTACCC 
TATACCAAAG ACGATGTGGA AGGTAATGTC AACTTGCGCG CTGGCGACAA GGTCAGCTTT 
CAAATCGCAA CGAACCAAAG AGGTAATCTA GGCGCCTGCC ATATTCGCCT GGAAAATCCG 
GCCCAGCCGG TTAAGTATCG TGGGGTTGTC TGCTCGATGA AGGAGTCGTT TGGCTTTATC 
GAACGTGCCG ATGTGGTCAA GGAGATTTTC TTCCACTTCT CAGAGGCTGA GGGCAATGTT 
GAGCTGCGTC CCGGTGACGA TGTCGAGTTC ACCATCCAGA CGCGGAGCGG CCGTGAATTT 
GCGTGCAACA TCACGCGCCT AGCTCCGGGA TCAGTGATCT TTGAGGACGT CGATAGCACG 
GTGTATAAGG GCCAGGTGCT TAAGTCGCTT GATCGCAACA ACCCGGTGCG CCAGAACAAT 
GATCCCTTGC CGGGTCGTAT TCGTTATAGG GCCTTGGATT ACTCCGAAGT GGAGGTTCCT 
TTCGGGGACA AGGATCAAAA GGGTGACTTC ACCCTGCGCC ACGGCGATTG GGTGCAGTTC 
CTGCTTGCCA CCGATCGGCG TGATCAATTG CAGAGGGCCA CCTCCATTGC CCTGTTGGAC 
GAGACTTTCA AGGTTTCCGG CGAGAAGCGG GAGCAGGGCA CCATTGCCTC TCTCAAGGAG 
GGCTTCGGAT TCCTGCGCTG CGTAGAGCGT CAGGCTCGTT TATTTTTCCA CTTTACTGAA 
GTTTTAGATA CGAGCCGCGA AATCGATATC AATGATGAGG TAGAATTCAC TGTCATTCAG 
GAGCCTGGAT TGGCCTATAA TAACTCGCGT TTGCAGGCCA TTCGCATTAA ACACTTGCCG 
CCCAACTCAG TGCAATTTGA AACTCTAGTG GCCAGCAACA TAGAAGGATG TGTAACTCGC 
GAGGCGCCTA AGAGCCCAAT TAAATCTCAA GATCGCGTCG AGGGCGGTGT GATTACCTAT 
GAACATGCCG ATGTTAAAAA GACTATAATG TATTTTCTTA AAGACTGCGA AAAACCACCA 
AGGATTGGGG AGCGCGTACG CTTCGATATT TACATGGTCA AACGTAACAA GGAGTGTATA 
GCCGTCAACG TACAGCAAGT ATCCCTGCAT CAGCAGCAAC AACAGCAGCA ACAGCAGTTG 
CATCTTAATC AGTCGAATGC TGGGGCAAAT ATCAACCAAA ATGACCAGCT GGGAGGATTG 
TCGAATGGAA TCAGTAGCAG CAGCTCGAAT GCTTCGCTAC AGAATGGCTA TGTCATGCAC 
GGCAGCCCTG GCGGCAGCAC CAGCAGCGTG GGCAGCAACA ATCCGGTTCA TCTGGATGAA 
TTCAAGATGG AGAACAACAA CCATGCCGGA TCCGATGCCG GTCAGGTGTA CCGTGGCTTC 
ATAGCCGTTA TGAAGGAGAA CTTTGGATTC ATTGAGACTT TGTCCCACGA TGAGGAGGTC 
TTCTTTCACT TTAGCAACTA TATGGGCAAT CCCAATTGGT TGGAGCTGGG TCAGGAGGTG 
GAGTACACTC TTGCTCGTAA TGGGAATACA TCCGTTTCTG GAAACTGTCT GCCTGCGGAA 
AATGTCCGTA TGCTGCCAAA GAACTCTATT CCCCAGCCGG CTGTGCTGGA AACCACACAC 
AATGGCGTGG TAGCACGTCC ACTGCGTTGC ATCAATCCCG ACCAGCAGGA ATATGCCGGT 
CTCATTGAAA TTCTCGATGA GCTACGTACC ACTGTCATTT CGCAACACGA ATTCGGGATT 
ACTAGTCTGG TGAACAAGAG GGATCTGCTC CAGAAGGGCG ATCTCGTTAG CTTCCGCATC 
GATGAGAGTG GTCGAGCAGC ATGCGTGAAT GCCGTAAGGC AGAAGAAGCG TGCCACTGTG 
GACTCCATCA AGGGTCAGTT TGGTTTTCTT AACTTTGAGG TGGAGGACGG CAAAAAACTG 
TTCTTCCACA TGTCTGAGGT GCAGGGCAAT ACTGTCGCTT TACATCCAGG CGATACCGTG 
GAGTTCTCCG TGGTCACCAA TCAGCGCAAT GGAAAGTCGT CGGCTTGCAA TGTTCTGAAA 
ATAAACGATC GTCCGGATCG TCTGATCTCG CGCCTTAAGC TCAACGGCGA TGACACTGTG 
CCGCGACTGA TCCTCATTCG CGCACCCAAG GGACCGCAGG GCAAGGGCTT TTCCGTTCTA 
GCTCGTCATC CACGCATTCC AGGCAACTTG GTGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246669.1
CDS	3599..6697
Translation	MNTQSKVYRTGEEIYDNLPCDSYFNMNAIRNLGIPTTFPTIGTFTLDSTTLGLQPQGQGPSPQQQQHQQQQQQQQQQHQQNMHHHQHQQQHMQQQQQQQQHQQQHQQQQHQQQQQHQQQQQQQQQHPTIGMFDANEVNDVIQNPPQIGVFQSNSVLTNGAGSGSSIFGSQSSNSSAAAADPSQTTRETGIIEKLLHSYGFIQCCERQARLFFHFSQFSGNIDHLKIGDPVEFEMTYDRRTGKPIASQVSKIAPEVVLSEERVTGTVTTELRTDSANNVLNSSETTGRISYENRGECFFLPYTKDDVEGNVNLRAGDKVSFQIATNQRGNLGACHIRLENPAQPVKYRGVVCSMKESFGFIERADVVKEIFFHFSEAEGNVELRPGDDVEFTIQTRSGREFACNITRLAPGSVIFEDVDSTVYKGQVLKSLDRNNPVRQNNDPLPGRIRYRALDYSEVEVPFGDKDQKGDFTLRHGDWVQFLLATDRRDQLQRATSIALLDETFKVSGEKREQGTIASLKEGFGFLRCVERQARLFFHFTEVLDTSREIDINDEVEFTVIQEPGLAYNNSRLQAIRIKHLPPNSVQFETLVASNIEGCVTREAPKSPIKSQDRVEGGVITYEHADVKKTIMYFLKDCEKPPRIGERVRFDIYMVKRNKECIAVNVQQVSLHQQQQQQQQQLHLNQSNAGANINQNDQLGGLSNGISSSSSNASLQNGYVMHGSPGGSTSSVGSNNPVHLDEFKMENNNHAGSDAGQVYRGFIAVMKENFGFIETLSHDEEVFFHFSNYMGNPNWLELGQEVEYTLARNGNTSVSGNCLPAENVRMLPKNSIPQPAVLETTHNGVVARPLRCINPDQQEYAGLIEILDELRTTVISQHEFGITSLVNKRDLLQKGDLVSFRIDESGRAACVNAVRQKKRATVDSIKGQFGFLNFEVEDGKKLFFHMSEVQGNTVALHPGDTVEFSVVTNQRNGKSSACNVLKINDRPDRLISRLKLNGDDTVPRLILIRAPKGPQGKGFSVLARHPRIPGNLVE

Target ORF information:

RefSeq Version	NM_001259740.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster upstream of N-ras, transcript variant C (Unr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259740.2

ORF Insert Sequence:

ATGAATACCC AATCGAAGGT GTACCGTACC GGCGAAGAAA TCTATGACAA CTTGCCATGC 
GATAGTTATT TCAACATGAA TGCCATTCGC AACCTGGGAA TCCCAACAAC GTTTCCGACC 
ATTGGAACAT TTACACTTGA CTCTACTACG TTGGGACTGC AGCCGCAGGG TCAGGGCCCG 
TCGCCCCAGC AGCAGCAGCA TCAACAGCAA CAACAACAGC AGCAGCAGCA ACATCAGCAG 
AATATGCACC ACCATCAACA TCAGCAGCAA CACATGCAGC AGCAGCAGCA ACAACAACAA 
CATCAGCAGC AACACCAACA GCAGCAACAT CAACAACAAC AGCAACATCA GCAGCAGCAG 
CAGCAGCAAC AACAGCATCC AACCATTGGA ATGTTCGATG CCAACGAGGT TAACGATGTC 
ATCCAGAATC CTCCTCAAAT CGGGGTATTC CAGTCTAACA GCGTTCTGAC CAACGGTGCC 
GGCAGTGGCT CCTCGATCTT TGGCTCCCAA TCGAGCAATT CATCGGCGGC AGCTGCAGAT 
CCCAGCCAGA CCACCCGGGA AACTGGCATC ATTGAAAAGT TGTTGCATTC GTATGGATTC 
ATTCAGTGCT GTGAGCGGCA GGCGCGTCTT TTCTTTCATT TCTCGCAATT CAGCGGTAAT 
ATTGATCATT TGAAAATCGG AGATCCCGTG GAGTTTGAGA TGACCTATGA TCGCCGCACC 
GGCAAGCCGA TAGCTAGTCA AGTATCAAAA ATAGCCCCAG AGGTTGTTCT CTCCGAGGAG 
CGGGTCACCG GCACGGTGAC CACCGAGCTG CGCACCGATA GCGCCAACAA TGTGCTTAAC 
TCCAGCGAGA CCACAGGTCG CATAAGCTAT GAGAACCGAG GCGAATGCTT CTTTTTACCC 
TATACCAAAG ACGATGTGGA AGGTAATGTC AACTTGCGCG CTGGCGACAA GGTCAGCTTT 
CAAATCGCAA CGAACCAAAG AGGTAATCTA GGCGCCTGCC ATATTCGCCT GGAAAATCCG 
GCCCAGCCGG TTAAGTATCG TGGGGTTGTC TGCTCGATGA AGGAGTCGTT TGGCTTTATC 
GAACGTGCCG ATGTGGTCAA GGAGATTTTC TTCCACTTCT CAGAGGCTGA GGGCAATGTT 
GAGCTGCGTC CCGGTGACGA TGTCGAGTTC ACCATCCAGA CGCGGAGCGG CCGTGAATTT 
GCGTGCAACA TCACGCGCCT AGCTCCGGGA TCAGTGATCT TTGAGGACGT CGATAGCACG 
GTGTATAAGG GCCAGGTGCT TAAGTCGCTT GATCGCAACA ACCCGGTGCG CCAGAACAAT 
GATCCCTTGC CGGGTCGTAT TCGTTATAGG GCCTTGGATT ACTCCGAAGT GGAGGTTCCT 
TTCGGGGACA AGGATCAAAA GGGTGACTTC ACCCTGCGCC ACGGCGATTG GGTGCAGTTC 
CTGCTTGCCA CCGATCGGCG TGATCAATTG CAGAGGGCCA CCTCCATTGC CCTGTTGGAC 
GAGACTTTCA AGGTTTCCGG CGAGAAGCGG GAGCAGGGCA CCATTGCCTC TCTCAAGGAG 
GGCTTCGGAT TCCTGCGCTG CGTAGAGCGT CAGGCTCGTT TATTTTTCCA CTTTACTGAA 
GTTTTAGATA CGAGCCGCGA AATCGATATC AATGATGAGG TAGAATTCAC TGTCATTCAG 
GAGCCTGGAT TGGCCTATAA TAACTCGCGT TTGCAGGCCA TTCGCATTAA ACACTTGCCG 
CCCAACTCAG TGCAATTTGA AACTCTAGTG GCCAGCAACA TAGAAGGATG TGTAACTCGC 
GAGGCGCCTA AGAGCCCAAT TAAATCTCAA GATCGCGTCG AGGGCGGTGT GATTACCTAT 
GAACATGCCG ATGTTAAAAA GACTATAATG TATTTTCTTA AAGACTGCGA AAAACCACCA 
AGGATTGGGG AGCGCGTACG CTTCGATATT TACATGGTCA AACGTAACAA GGAGTGTATA 
GCCGTCAACG TACAGCAAGT ATCCCTGCAT CAGCAGCAAC AACAGCAGCA ACAGCAGTTG 
CATCTTAATC AGTCGAATGC TGGGGCAAAT ATCAACCAAA ATGACCAGCT GGGAGGATTG 
TCGAATGGAA TCAGTAGCAG CAGCTCGAAT GCTTCGCTAC AGAATGGCTA TGTCATGCAC 
GGCAGCCCTG GCGGCAGCAC CAGCAGCGTG GGCAGCAACA ATCCGGTTCA TCTGGATGAA 
TTCAAGATGG AGAACAACAA CCATGCCGGA TCCGATGCCG GTCAGGTGTA CCGTGGCTTC 
ATAGCCGTTA TGAAGGAGAA CTTTGGATTC ATTGAGACTT TGTCCCACGA TGAGGAGGTC 
TTCTTTCACT TTAGCAACTA TATGGGCAAT CCCAATTGGT TGGAGCTGGG TCAGGAGGTG 
GAGTACACTC TTGCTCGTAA TGGGAATACA TCCGTTTCTG GAAACTGTCT GCCTGCGGAA 
AATGTCCGTA TGCTGCCAAA GAACTCTATT CCCCAGCCGG CTGTGCTGGA AACCACACAC 
AATGGCGTGG TAGCACGTCC ACTGCGTTGC ATCAATCCCG ACCAGCAGGA ATATGCCGGT 
CTCATTGAAA TTCTCGATGA GCTACGTACC ACTGTCATTT CGCAACACGA ATTCGGGATT 
ACTAGTCTGG TGAACAAGAG GGATCTGCTC CAGAAGGGCG ATCTCGTTAG CTTCCGCATC 
GATGAGAGTG GTCGAGCAGC ATGCGTGAAT GCCGTAAGGC AGAAGAAGCG TGCCACTGTG 
GACTCCATCA AGGGTCAGTT TGGTTTTCTT AACTTTGAGG TGGAGGACGG CAAAAAACTG 
TTCTTCCACA TGTCTGAGGT GCAGGGCAAT ACTGTCGCTT TACATCCAGG CGATACCGTG 
GAGTTCTCCG TGGTCACCAA TCAGCGCAAT GGAAAGTCGT CGGCTTGCAA TGTTCTGAAA 
ATAAACGATC GTCCGGATCG TCTGATCTCG CGCCTTAAGC TCAACGGCGA TGACACTGTG 
CCGCGACTGA TCCTCATTCG CGCACCCAAG GGACCGCAGG GCAAGGGCTT TTCCGTTCTA 
GCTCGTCATC CACGCATTCC AGGCAACTTG GTGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unr ( NM_001274650.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261579.1 Drosophila melanogaster upstream of N-ras, transcript variant D (Unr), mRNA.

Price & Availability↑

CloneID	OFa17891
Clone ID Related Accession (Same CDS sequence)	NM_001274650.1 , NM_001300054.1 , NM_001144440.2
Accession Version	NM_001274650.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	upstream of N-ras, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATACCC AATCGAAGGT GTACCGTACC GGCGAAGAAA TCTATGACAA CTTGCCATGC 
GATAGTTATT TCAACATGAA TGCCATTCGC AACCTGGGAA TCCCAACAAC GTTTCCGACC 
ATTGGAACAT TTACACTTGA CTCTACTACG TTGGGACTGC AGCCGCAGGG TCAGGGCCCG 
TCGCCCCAGC AGCAGCAGCA TCAACAGCAA CAACAACAGC AGCAGCAGCA ACATCAGCAG 
AATATGCACC ACCATCAACA TCAGCAGCAA CACATGCAGC AGCAGCAGCA ACAACAACAA 
CATCAGCAGC AACACCAACA GCAGCAACAT CAACAACAAC AGCAACATCA GCAGCAGCAG 
CAGCAGCAAC AACAGCATCC AACCATTGGA ATGTTCGATG CCAACGAGGT TAACGATGTC 
ATCCAGAATC CTCCTCAAAT CGGGGTATTC CAGTCTAACA GCGTTCTGAC CAACGGTGCC 
GGCAGTGGCT CCTCGATCTT TGGCTCCCAA TCGAGCAATT CATCGGCGGC AGCTGCAGAT 
CCCAGCCAGA CCACCCGGGA AACTGGCATC ATTGAAAAGT TGTTGCATTC GTATGGATTC 
ATTCAGTGCT GTGAGCGGCA GGCGCGTCTT TTCTTTCATT TCTCGCAATT CAGCGGTAAT 
ATTGATCATT TGAAAATCGG AGATCCCGTG GAGTTTGAGA TGACCTATGA TCGCCGCACC 
GGCAAGCCGA TAGCTAGTCA AGTATCAAAA ATAGCCCCAG AGGTTGTTCT CTCCGAGGAG 
CGGGTCACCG GCACGGTGAC CACCGAGCTG CGCACCGATA GCGCCAACAA TGTGCTTAAC 
TCCAGCGAGA CCACAGGTCG CATAAGCTAT GAGAACCGAG GCGAATGCTT CTTTTTACCC 
TATACCAAAG ACGATGTGGA AGGTAATGTC AACTTGCGCG CTGGCGACAA GGTCAGCTTT 
CAAATCGCAA CGAACCAAAG AGGTAATCTA GGCGCCTGCC ATATTCGCCT GGAAAATCCG 
GCCCAGCCGG TTAAGTATCG TGGGGTTGTC TGCTCGATGA AGGAGTCGTT TGGCTTTATC 
GAACGTGCCG ATGTGGTCAA GGAGATTTTC TTCCACTTCT CAGAGGCTGA GGGCAATGTT 
GAGCTGCGTC CCGGTGACGA TGTCGAGTTC ACCATCCAGA CGCGGAGCGT AAGTGGCAGT 
GGCGATCCGA CCAATCCCAA TATGCTCCAA TCAAAAATGC AGTCTGCATC TGTGCCTCCG 
CAGGGCCGTG AATTTGCGTG CAACATCACG CGCCTAGCTC CGGGATCAGT GATCTTTGAG 
GACGTCGATA GCACGGTGTA TAAGGGCCAG GTGCTTAAGT CGCTTGATCG CAACAACCCG 
GTGCGCCAGA ACAATGATCC CTTGCCGGGT CGTATTCGTT ATAGGGCCTT GGATTACTCC 
GAAGTGGAGG TTCCTTTCGG GGACAAGGAT CAAAAGGGTG ACTTCACCCT GCGCCACGGC 
GATTGGGTGC AGTTCCTGCT TGCCACCGAT CGGCGTGATC AATTGCAGAG GGCCACCTCC 
ATTGCCCTGT TGGACGAGAC TTTCAAGGTT TCCGGCGAGA AGCGGGAGCA GGGCACCATT 
GCCTCTCTCA AGGAGGGCTT CGGATTCCTG CGCTGCGTAG AGCGTCAGGC TCGTTTATTT 
TTCCACTTTA CTGAAGTTTT AGATACGAGC CGCGAAATCG ATATCAATGA TGAGGTAGAA 
TTCACTGTCA TTCAGGAGCC TGGATTGGCC TATAATAACT CGCGTTTGCA GGCCATTCGC 
ATTAAACACT TGCCGCCCAA CTCAGTGCAA TTTGAAACTC TAGTGGCCAG CAACATAGAA 
GGATGTGTAA CTCGCGAGGC GCCTAAGAGC CCAATTAAAT CTCAAGATCG CGTCGAGGGC 
GGTGTGATTA CCTATGAACA TGCCGATGTT AAAAAGACTA TAATGTATTT TCTTAAAGAC 
TGCGAAAAAC CACCAAGGAT TGGGGAGCGC GTACGCTTCG ATATTTACAT GGTCAAACGT 
AACAAGGAGT GTATAGCCGT CAACGTACAG CAAGTATCCC TGCATCAGCA GCAACAACAG 
CAGCAACAGC AGTTGCATCT TAATCAGTCG AATGCTGGGG CAAATATCAA CCAAAATGAC 
CAGCTGGGAG GATTGTCGAA TGGAATCAGT AGCAGCAGCT CGAATGCTTC GCTACAGAAT 
GGCTATGTCA TGCACGGCAG CCCTGGCGGC AGCACCAGCA GCGTGGGCAG CAACAATCCG 
GTTCATCTGG ATGAATTCAA GATGGAGAAC AACAACCATG CCGGATCCGA TGCCGGTCAG 
GTGTACCGTG GCTTCATAGC CGTTATGAAG GAGAACTTTG GATTCATTGA GACTTTGTCC 
CACGATGAGG AGGTCTTCTT TCACTTTAGC AACTATATGG GCAATCCCAA TTGGTTGGAG 
CTGGGTCAGG AGGTGGAGTA CACTCTTGCT CGTAATGGGA ATACATCCGT TTCTGGAAAC 
TGTCTGCCTG CGGAAAATGT CCGTATGCTG CCAAAGAACT CTATTCCCCA GCCGGCTGTG 
CTGGAAACCA CACACAATGG CGTGGTAGCA CGTCCACTGC GTTGCATCAA TCCCGACCAG 
CAGGAATATG CCGGTCTCAT TGAAATTCTC GATGAGCTAC GTACCACTGT CATTTCGCAA 
CACGAATTCG GGATTACTAG TCTGGTGAAC AAGAGGGATC TGCTCCAGAA GGGCGATCTC 
GTTAGCTTCC GCATCGATGA GAGTGGTCGA GCAGCATGCG TGAATGCCGT AAGGCAGAAG 
AAGCGTGCCA CTGTGGACTC CATCAAGGGT CAGTTTGGTT TTCTTAACTT TGAGGTGGAG 
GACGGCAAAA AACTGTTCTT CCACATGTCT GAGGTGCAGG GCAATACTGT CGCTTTACAT 
CCAGGCGATA CCGTGGAGTT CTCCGTGGTC ACCAATCAGC GCAATGGAAA GTCGTCGGCT 
TGCAATGTTC TGAAAATAAA CGATCGTCCG GATCGTCTGA TCTCGCGCCT TAAGCTCAAC 
GGCGATGACA CTGTGCCGCG ACTGATCCTC ATTCGCGCAC CCAAGGGACC GCAGGGCAAG 
GGCTTTTCCG TTCTAGCTCG TCATCCACGC ATTCCAGGCA ACTTGGTGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261579.1
CDS	462..3635
Translation	MNTQSKVYRTGEEIYDNLPCDSYFNMNAIRNLGIPTTFPTIGTFTLDSTTLGLQPQGQGPSPQQQQHQQQQQQQQQQHQQNMHHHQHQQQHMQQQQQQQQHQQQHQQQQHQQQQQHQQQQQQQQQHPTIGMFDANEVNDVIQNPPQIGVFQSNSVLTNGAGSGSSIFGSQSSNSSAAAADPSQTTRETGIIEKLLHSYGFIQCCERQARLFFHFSQFSGNIDHLKIGDPVEFEMTYDRRTGKPIASQVSKIAPEVVLSEERVTGTVTTELRTDSANNVLNSSETTGRISYENRGECFFLPYTKDDVEGNVNLRAGDKVSFQIATNQRGNLGACHIRLENPAQPVKYRGVVCSMKESFGFIERADVVKEIFFHFSEAEGNVELRPGDDVEFTIQTRSVSGSGDPTNPNMLQSKMQSASVPPQGREFACNITRLAPGSVIFEDVDSTVYKGQVLKSLDRNNPVRQNNDPLPGRIRYRALDYSEVEVPFGDKDQKGDFTLRHGDWVQFLLATDRRDQLQRATSIALLDETFKVSGEKREQGTIASLKEGFGFLRCVERQARLFFHFTEVLDTSREIDINDEVEFTVIQEPGLAYNNSRLQAIRIKHLPPNSVQFETLVASNIEGCVTREAPKSPIKSQDRVEGGVITYEHADVKKTIMYFLKDCEKPPRIGERVRFDIYMVKRNKECIAVNVQQVSLHQQQQQQQQQLHLNQSNAGANINQNDQLGGLSNGISSSSSNASLQNGYVMHGSPGGSTSSVGSNNPVHLDEFKMENNNHAGSDAGQVYRGFIAVMKENFGFIETLSHDEEVFFHFSNYMGNPNWLELGQEVEYTLARNGNTSVSGNCLPAENVRMLPKNSIPQPAVLETTHNGVVARPLRCINPDQQEYAGLIEILDELRTTVISQHEFGITSLVNKRDLLQKGDLVSFRIDESGRAACVNAVRQKKRATVDSIKGQFGFLNFEVEDGKKLFFHMSEVQGNTVALHPGDTVEFSVVTNQRNGKSSACNVLKINDRPDRLISRLKLNGDDTVPRLILIRAPKGPQGKGFSVLARHPRIPGNLVE

Target ORF information:

RefSeq Version	NM_001274650.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster upstream of N-ras, transcript variant D (Unr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274650.1

ORF Insert Sequence:

ATGAATACCC AATCGAAGGT GTACCGTACC GGCGAAGAAA TCTATGACAA CTTGCCATGC 
GATAGTTATT TCAACATGAA TGCCATTCGC AACCTGGGAA TCCCAACAAC GTTTCCGACC 
ATTGGAACAT TTACACTTGA CTCTACTACG TTGGGACTGC AGCCGCAGGG TCAGGGCCCG 
TCGCCCCAGC AGCAGCAGCA TCAACAGCAA CAACAACAGC AGCAGCAGCA ACATCAGCAG 
AATATGCACC ACCATCAACA TCAGCAGCAA CACATGCAGC AGCAGCAGCA ACAACAACAA 
CATCAGCAGC AACACCAACA GCAGCAACAT CAACAACAAC AGCAACATCA GCAGCAGCAG 
CAGCAGCAAC AACAGCATCC AACCATTGGA ATGTTCGATG CCAACGAGGT TAACGATGTC 
ATCCAGAATC CTCCTCAAAT CGGGGTATTC CAGTCTAACA GCGTTCTGAC CAACGGTGCC 
GGCAGTGGCT CCTCGATCTT TGGCTCCCAA TCGAGCAATT CATCGGCGGC AGCTGCAGAT 
CCCAGCCAGA CCACCCGGGA AACTGGCATC ATTGAAAAGT TGTTGCATTC GTATGGATTC 
ATTCAGTGCT GTGAGCGGCA GGCGCGTCTT TTCTTTCATT TCTCGCAATT CAGCGGTAAT 
ATTGATCATT TGAAAATCGG AGATCCCGTG GAGTTTGAGA TGACCTATGA TCGCCGCACC 
GGCAAGCCGA TAGCTAGTCA AGTATCAAAA ATAGCCCCAG AGGTTGTTCT CTCCGAGGAG 
CGGGTCACCG GCACGGTGAC CACCGAGCTG CGCACCGATA GCGCCAACAA TGTGCTTAAC 
TCCAGCGAGA CCACAGGTCG CATAAGCTAT GAGAACCGAG GCGAATGCTT CTTTTTACCC 
TATACCAAAG ACGATGTGGA AGGTAATGTC AACTTGCGCG CTGGCGACAA GGTCAGCTTT 
CAAATCGCAA CGAACCAAAG AGGTAATCTA GGCGCCTGCC ATATTCGCCT GGAAAATCCG 
GCCCAGCCGG TTAAGTATCG TGGGGTTGTC TGCTCGATGA AGGAGTCGTT TGGCTTTATC 
GAACGTGCCG ATGTGGTCAA GGAGATTTTC TTCCACTTCT CAGAGGCTGA GGGCAATGTT 
GAGCTGCGTC CCGGTGACGA TGTCGAGTTC ACCATCCAGA CGCGGAGCGT AAGTGGCAGT 
GGCGATCCGA CCAATCCCAA TATGCTCCAA TCAAAAATGC AGTCTGCATC TGTGCCTCCG 
CAGGGCCGTG AATTTGCGTG CAACATCACG CGCCTAGCTC CGGGATCAGT GATCTTTGAG 
GACGTCGATA GCACGGTGTA TAAGGGCCAG GTGCTTAAGT CGCTTGATCG CAACAACCCG 
GTGCGCCAGA ACAATGATCC CTTGCCGGGT CGTATTCGTT ATAGGGCCTT GGATTACTCC 
GAAGTGGAGG TTCCTTTCGG GGACAAGGAT CAAAAGGGTG ACTTCACCCT GCGCCACGGC 
GATTGGGTGC AGTTCCTGCT TGCCACCGAT CGGCGTGATC AATTGCAGAG GGCCACCTCC 
ATTGCCCTGT TGGACGAGAC TTTCAAGGTT TCCGGCGAGA AGCGGGAGCA GGGCACCATT 
GCCTCTCTCA AGGAGGGCTT CGGATTCCTG CGCTGCGTAG AGCGTCAGGC TCGTTTATTT 
TTCCACTTTA CTGAAGTTTT AGATACGAGC CGCGAAATCG ATATCAATGA TGAGGTAGAA 
TTCACTGTCA TTCAGGAGCC TGGATTGGCC TATAATAACT CGCGTTTGCA GGCCATTCGC 
ATTAAACACT TGCCGCCCAA CTCAGTGCAA TTTGAAACTC TAGTGGCCAG CAACATAGAA 
GGATGTGTAA CTCGCGAGGC GCCTAAGAGC CCAATTAAAT CTCAAGATCG CGTCGAGGGC 
GGTGTGATTA CCTATGAACA TGCCGATGTT AAAAAGACTA TAATGTATTT TCTTAAAGAC 
TGCGAAAAAC CACCAAGGAT TGGGGAGCGC GTACGCTTCG ATATTTACAT GGTCAAACGT 
AACAAGGAGT GTATAGCCGT CAACGTACAG CAAGTATCCC TGCATCAGCA GCAACAACAG 
CAGCAACAGC AGTTGCATCT TAATCAGTCG AATGCTGGGG CAAATATCAA CCAAAATGAC 
CAGCTGGGAG GATTGTCGAA TGGAATCAGT AGCAGCAGCT CGAATGCTTC GCTACAGAAT 
GGCTATGTCA TGCACGGCAG CCCTGGCGGC AGCACCAGCA GCGTGGGCAG CAACAATCCG 
GTTCATCTGG ATGAATTCAA GATGGAGAAC AACAACCATG CCGGATCCGA TGCCGGTCAG 
GTGTACCGTG GCTTCATAGC CGTTATGAAG GAGAACTTTG GATTCATTGA GACTTTGTCC 
CACGATGAGG AGGTCTTCTT TCACTTTAGC AACTATATGG GCAATCCCAA TTGGTTGGAG 
CTGGGTCAGG AGGTGGAGTA CACTCTTGCT CGTAATGGGA ATACATCCGT TTCTGGAAAC 
TGTCTGCCTG CGGAAAATGT CCGTATGCTG CCAAAGAACT CTATTCCCCA GCCGGCTGTG 
CTGGAAACCA CACACAATGG CGTGGTAGCA CGTCCACTGC GTTGCATCAA TCCCGACCAG 
CAGGAATATG CCGGTCTCAT TGAAATTCTC GATGAGCTAC GTACCACTGT CATTTCGCAA 
CACGAATTCG GGATTACTAG TCTGGTGAAC AAGAGGGATC TGCTCCAGAA GGGCGATCTC 
GTTAGCTTCC GCATCGATGA GAGTGGTCGA GCAGCATGCG TGAATGCCGT AAGGCAGAAG 
AAGCGTGCCA CTGTGGACTC CATCAAGGGT CAGTTTGGTT TTCTTAACTT TGAGGTGGAG 
GACGGCAAAA AACTGTTCTT CCACATGTCT GAGGTGCAGG GCAATACTGT CGCTTTACAT 
CCAGGCGATA CCGTGGAGTT CTCCGTGGTC ACCAATCAGC GCAATGGAAA GTCGTCGGCT 
TGCAATGTTC TGAAAATAAA CGATCGTCCG GATCGTCTGA TCTCGCGCCT TAAGCTCAAC 
GGCGATGACA CTGTGCCGCG ACTGATCCTC ATTCGCGCAC CCAAGGGACC GCAGGGCAAG 
GGCTTTTCCG TTCTAGCTCG TCATCCACGC ATTCCAGGCA ACTTGGTGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unr ( NM_001300054.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286983.1 Drosophila melanogaster upstream of N-ras, transcript variant E (Unr), mRNA.

Price & Availability↑

CloneID	OFa17891
Clone ID Related Accession (Same CDS sequence)	NM_001274650.1 , NM_001300054.1 , NM_001144440.2
Accession Version	NM_001300054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	upstream of N-ras, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATACCC AATCGAAGGT GTACCGTACC GGCGAAGAAA TCTATGACAA CTTGCCATGC 
GATAGTTATT TCAACATGAA TGCCATTCGC AACCTGGGAA TCCCAACAAC GTTTCCGACC 
ATTGGAACAT TTACACTTGA CTCTACTACG TTGGGACTGC AGCCGCAGGG TCAGGGCCCG 
TCGCCCCAGC AGCAGCAGCA TCAACAGCAA CAACAACAGC AGCAGCAGCA ACATCAGCAG 
AATATGCACC ACCATCAACA TCAGCAGCAA CACATGCAGC AGCAGCAGCA ACAACAACAA 
CATCAGCAGC AACACCAACA GCAGCAACAT CAACAACAAC AGCAACATCA GCAGCAGCAG 
CAGCAGCAAC AACAGCATCC AACCATTGGA ATGTTCGATG CCAACGAGGT TAACGATGTC 
ATCCAGAATC CTCCTCAAAT CGGGGTATTC CAGTCTAACA GCGTTCTGAC CAACGGTGCC 
GGCAGTGGCT CCTCGATCTT TGGCTCCCAA TCGAGCAATT CATCGGCGGC AGCTGCAGAT 
CCCAGCCAGA CCACCCGGGA AACTGGCATC ATTGAAAAGT TGTTGCATTC GTATGGATTC 
ATTCAGTGCT GTGAGCGGCA GGCGCGTCTT TTCTTTCATT TCTCGCAATT CAGCGGTAAT 
ATTGATCATT TGAAAATCGG AGATCCCGTG GAGTTTGAGA TGACCTATGA TCGCCGCACC 
GGCAAGCCGA TAGCTAGTCA AGTATCAAAA ATAGCCCCAG AGGTTGTTCT CTCCGAGGAG 
CGGGTCACCG GCACGGTGAC CACCGAGCTG CGCACCGATA GCGCCAACAA TGTGCTTAAC 
TCCAGCGAGA CCACAGGTCG CATAAGCTAT GAGAACCGAG GCGAATGCTT CTTTTTACCC 
TATACCAAAG ACGATGTGGA AGGTAATGTC AACTTGCGCG CTGGCGACAA GGTCAGCTTT 
CAAATCGCAA CGAACCAAAG AGGTAATCTA GGCGCCTGCC ATATTCGCCT GGAAAATCCG 
GCCCAGCCGG TTAAGTATCG TGGGGTTGTC TGCTCGATGA AGGAGTCGTT TGGCTTTATC 
GAACGTGCCG ATGTGGTCAA GGAGATTTTC TTCCACTTCT CAGAGGCTGA GGGCAATGTT 
GAGCTGCGTC CCGGTGACGA TGTCGAGTTC ACCATCCAGA CGCGGAGCGT AAGTGGCAGT 
GGCGATCCGA CCAATCCCAA TATGCTCCAA TCAAAAATGC AGTCTGCATC TGTGCCTCCG 
CAGGGCCGTG AATTTGCGTG CAACATCACG CGCCTAGCTC CGGGATCAGT GATCTTTGAG 
GACGTCGATA GCACGGTGTA TAAGGGCCAG GTGCTTAAGT CGCTTGATCG CAACAACCCG 
GTGCGCCAGA ACAATGATCC CTTGCCGGGT CGTATTCGTT ATAGGGCCTT GGATTACTCC 
GAAGTGGAGG TTCCTTTCGG GGACAAGGAT CAAAAGGGTG ACTTCACCCT GCGCCACGGC 
GATTGGGTGC AGTTCCTGCT TGCCACCGAT CGGCGTGATC AATTGCAGAG GGCCACCTCC 
ATTGCCCTGT TGGACGAGAC TTTCAAGGTT TCCGGCGAGA AGCGGGAGCA GGGCACCATT 
GCCTCTCTCA AGGAGGGCTT CGGATTCCTG CGCTGCGTAG AGCGTCAGGC TCGTTTATTT 
TTCCACTTTA CTGAAGTTTT AGATACGAGC CGCGAAATCG ATATCAATGA TGAGGTAGAA 
TTCACTGTCA TTCAGGAGCC TGGATTGGCC TATAATAACT CGCGTTTGCA GGCCATTCGC 
ATTAAACACT TGCCGCCCAA CTCAGTGCAA TTTGAAACTC TAGTGGCCAG CAACATAGAA 
GGATGTGTAA CTCGCGAGGC GCCTAAGAGC CCAATTAAAT CTCAAGATCG CGTCGAGGGC 
GGTGTGATTA CCTATGAACA TGCCGATGTT AAAAAGACTA TAATGTATTT TCTTAAAGAC 
TGCGAAAAAC CACCAAGGAT TGGGGAGCGC GTACGCTTCG ATATTTACAT GGTCAAACGT 
AACAAGGAGT GTATAGCCGT CAACGTACAG CAAGTATCCC TGCATCAGCA GCAACAACAG 
CAGCAACAGC AGTTGCATCT TAATCAGTCG AATGCTGGGG CAAATATCAA CCAAAATGAC 
CAGCTGGGAG GATTGTCGAA TGGAATCAGT AGCAGCAGCT CGAATGCTTC GCTACAGAAT 
GGCTATGTCA TGCACGGCAG CCCTGGCGGC AGCACCAGCA GCGTGGGCAG CAACAATCCG 
GTTCATCTGG ATGAATTCAA GATGGAGAAC AACAACCATG CCGGATCCGA TGCCGGTCAG 
GTGTACCGTG GCTTCATAGC CGTTATGAAG GAGAACTTTG GATTCATTGA GACTTTGTCC 
CACGATGAGG AGGTCTTCTT TCACTTTAGC AACTATATGG GCAATCCCAA TTGGTTGGAG 
CTGGGTCAGG AGGTGGAGTA CACTCTTGCT CGTAATGGGA ATACATCCGT TTCTGGAAAC 
TGTCTGCCTG CGGAAAATGT CCGTATGCTG CCAAAGAACT CTATTCCCCA GCCGGCTGTG 
CTGGAAACCA CACACAATGG CGTGGTAGCA CGTCCACTGC GTTGCATCAA TCCCGACCAG 
CAGGAATATG CCGGTCTCAT TGAAATTCTC GATGAGCTAC GTACCACTGT CATTTCGCAA 
CACGAATTCG GGATTACTAG TCTGGTGAAC AAGAGGGATC TGCTCCAGAA GGGCGATCTC 
GTTAGCTTCC GCATCGATGA GAGTGGTCGA GCAGCATGCG TGAATGCCGT AAGGCAGAAG 
AAGCGTGCCA CTGTGGACTC CATCAAGGGT CAGTTTGGTT TTCTTAACTT TGAGGTGGAG 
GACGGCAAAA AACTGTTCTT CCACATGTCT GAGGTGCAGG GCAATACTGT CGCTTTACAT 
CCAGGCGATA CCGTGGAGTT CTCCGTGGTC ACCAATCAGC GCAATGGAAA GTCGTCGGCT 
TGCAATGTTC TGAAAATAAA CGATCGTCCG GATCGTCTGA TCTCGCGCCT TAAGCTCAAC 
GGCGATGACA CTGTGCCGCG ACTGATCCTC ATTCGCGCAC CCAAGGGACC GCAGGGCAAG 
GGCTTTTCCG TTCTAGCTCG TCATCCACGC ATTCCAGGCA ACTTGGTGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286983.1
CDS	3479..6652
Translation	MNTQSKVYRTGEEIYDNLPCDSYFNMNAIRNLGIPTTFPTIGTFTLDSTTLGLQPQGQGPSPQQQQHQQQQQQQQQQHQQNMHHHQHQQQHMQQQQQQQQHQQQHQQQQHQQQQQHQQQQQQQQQHPTIGMFDANEVNDVIQNPPQIGVFQSNSVLTNGAGSGSSIFGSQSSNSSAAAADPSQTTRETGIIEKLLHSYGFIQCCERQARLFFHFSQFSGNIDHLKIGDPVEFEMTYDRRTGKPIASQVSKIAPEVVLSEERVTGTVTTELRTDSANNVLNSSETTGRISYENRGECFFLPYTKDDVEGNVNLRAGDKVSFQIATNQRGNLGACHIRLENPAQPVKYRGVVCSMKESFGFIERADVVKEIFFHFSEAEGNVELRPGDDVEFTIQTRSVSGSGDPTNPNMLQSKMQSASVPPQGREFACNITRLAPGSVIFEDVDSTVYKGQVLKSLDRNNPVRQNNDPLPGRIRYRALDYSEVEVPFGDKDQKGDFTLRHGDWVQFLLATDRRDQLQRATSIALLDETFKVSGEKREQGTIASLKEGFGFLRCVERQARLFFHFTEVLDTSREIDINDEVEFTVIQEPGLAYNNSRLQAIRIKHLPPNSVQFETLVASNIEGCVTREAPKSPIKSQDRVEGGVITYEHADVKKTIMYFLKDCEKPPRIGERVRFDIYMVKRNKECIAVNVQQVSLHQQQQQQQQQLHLNQSNAGANINQNDQLGGLSNGISSSSSNASLQNGYVMHGSPGGSTSSVGSNNPVHLDEFKMENNNHAGSDAGQVYRGFIAVMKENFGFIETLSHDEEVFFHFSNYMGNPNWLELGQEVEYTLARNGNTSVSGNCLPAENVRMLPKNSIPQPAVLETTHNGVVARPLRCINPDQQEYAGLIEILDELRTTVISQHEFGITSLVNKRDLLQKGDLVSFRIDESGRAACVNAVRQKKRATVDSIKGQFGFLNFEVEDGKKLFFHMSEVQGNTVALHPGDTVEFSVVTNQRNGKSSACNVLKINDRPDRLISRLKLNGDDTVPRLILIRAPKGPQGKGFSVLARHPRIPGNLVE

Target ORF information:

RefSeq Version	NM_001300054.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster upstream of N-ras, transcript variant E (Unr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300054.1

ORF Insert Sequence:

ATGAATACCC AATCGAAGGT GTACCGTACC GGCGAAGAAA TCTATGACAA CTTGCCATGC 
GATAGTTATT TCAACATGAA TGCCATTCGC AACCTGGGAA TCCCAACAAC GTTTCCGACC 
ATTGGAACAT TTACACTTGA CTCTACTACG TTGGGACTGC AGCCGCAGGG TCAGGGCCCG 
TCGCCCCAGC AGCAGCAGCA TCAACAGCAA CAACAACAGC AGCAGCAGCA ACATCAGCAG 
AATATGCACC ACCATCAACA TCAGCAGCAA CACATGCAGC AGCAGCAGCA ACAACAACAA 
CATCAGCAGC AACACCAACA GCAGCAACAT CAACAACAAC AGCAACATCA GCAGCAGCAG 
CAGCAGCAAC AACAGCATCC AACCATTGGA ATGTTCGATG CCAACGAGGT TAACGATGTC 
ATCCAGAATC CTCCTCAAAT CGGGGTATTC CAGTCTAACA GCGTTCTGAC CAACGGTGCC 
GGCAGTGGCT CCTCGATCTT TGGCTCCCAA TCGAGCAATT CATCGGCGGC AGCTGCAGAT 
CCCAGCCAGA CCACCCGGGA AACTGGCATC ATTGAAAAGT TGTTGCATTC GTATGGATTC 
ATTCAGTGCT GTGAGCGGCA GGCGCGTCTT TTCTTTCATT TCTCGCAATT CAGCGGTAAT 
ATTGATCATT TGAAAATCGG AGATCCCGTG GAGTTTGAGA TGACCTATGA TCGCCGCACC 
GGCAAGCCGA TAGCTAGTCA AGTATCAAAA ATAGCCCCAG AGGTTGTTCT CTCCGAGGAG 
CGGGTCACCG GCACGGTGAC CACCGAGCTG CGCACCGATA GCGCCAACAA TGTGCTTAAC 
TCCAGCGAGA CCACAGGTCG CATAAGCTAT GAGAACCGAG GCGAATGCTT CTTTTTACCC 
TATACCAAAG ACGATGTGGA AGGTAATGTC AACTTGCGCG CTGGCGACAA GGTCAGCTTT 
CAAATCGCAA CGAACCAAAG AGGTAATCTA GGCGCCTGCC ATATTCGCCT GGAAAATCCG 
GCCCAGCCGG TTAAGTATCG TGGGGTTGTC TGCTCGATGA AGGAGTCGTT TGGCTTTATC 
GAACGTGCCG ATGTGGTCAA GGAGATTTTC TTCCACTTCT CAGAGGCTGA GGGCAATGTT 
GAGCTGCGTC CCGGTGACGA TGTCGAGTTC ACCATCCAGA CGCGGAGCGT AAGTGGCAGT 
GGCGATCCGA CCAATCCCAA TATGCTCCAA TCAAAAATGC AGTCTGCATC TGTGCCTCCG 
CAGGGCCGTG AATTTGCGTG CAACATCACG CGCCTAGCTC CGGGATCAGT GATCTTTGAG 
GACGTCGATA GCACGGTGTA TAAGGGCCAG GTGCTTAAGT CGCTTGATCG CAACAACCCG 
GTGCGCCAGA ACAATGATCC CTTGCCGGGT CGTATTCGTT ATAGGGCCTT GGATTACTCC 
GAAGTGGAGG TTCCTTTCGG GGACAAGGAT CAAAAGGGTG ACTTCACCCT GCGCCACGGC 
GATTGGGTGC AGTTCCTGCT TGCCACCGAT CGGCGTGATC AATTGCAGAG GGCCACCTCC 
ATTGCCCTGT TGGACGAGAC TTTCAAGGTT TCCGGCGAGA AGCGGGAGCA GGGCACCATT 
GCCTCTCTCA AGGAGGGCTT CGGATTCCTG CGCTGCGTAG AGCGTCAGGC TCGTTTATTT 
TTCCACTTTA CTGAAGTTTT AGATACGAGC CGCGAAATCG ATATCAATGA TGAGGTAGAA 
TTCACTGTCA TTCAGGAGCC TGGATTGGCC TATAATAACT CGCGTTTGCA GGCCATTCGC 
ATTAAACACT TGCCGCCCAA CTCAGTGCAA TTTGAAACTC TAGTGGCCAG CAACATAGAA 
GGATGTGTAA CTCGCGAGGC GCCTAAGAGC CCAATTAAAT CTCAAGATCG CGTCGAGGGC 
GGTGTGATTA CCTATGAACA TGCCGATGTT AAAAAGACTA TAATGTATTT TCTTAAAGAC 
TGCGAAAAAC CACCAAGGAT TGGGGAGCGC GTACGCTTCG ATATTTACAT GGTCAAACGT 
AACAAGGAGT GTATAGCCGT CAACGTACAG CAAGTATCCC TGCATCAGCA GCAACAACAG 
CAGCAACAGC AGTTGCATCT TAATCAGTCG AATGCTGGGG CAAATATCAA CCAAAATGAC 
CAGCTGGGAG GATTGTCGAA TGGAATCAGT AGCAGCAGCT CGAATGCTTC GCTACAGAAT 
GGCTATGTCA TGCACGGCAG CCCTGGCGGC AGCACCAGCA GCGTGGGCAG CAACAATCCG 
GTTCATCTGG ATGAATTCAA GATGGAGAAC AACAACCATG CCGGATCCGA TGCCGGTCAG 
GTGTACCGTG GCTTCATAGC CGTTATGAAG GAGAACTTTG GATTCATTGA GACTTTGTCC 
CACGATGAGG AGGTCTTCTT TCACTTTAGC AACTATATGG GCAATCCCAA TTGGTTGGAG 
CTGGGTCAGG AGGTGGAGTA CACTCTTGCT CGTAATGGGA ATACATCCGT TTCTGGAAAC 
TGTCTGCCTG CGGAAAATGT CCGTATGCTG CCAAAGAACT CTATTCCCCA GCCGGCTGTG 
CTGGAAACCA CACACAATGG CGTGGTAGCA CGTCCACTGC GTTGCATCAA TCCCGACCAG 
CAGGAATATG CCGGTCTCAT TGAAATTCTC GATGAGCTAC GTACCACTGT CATTTCGCAA 
CACGAATTCG GGATTACTAG TCTGGTGAAC AAGAGGGATC TGCTCCAGAA GGGCGATCTC 
GTTAGCTTCC GCATCGATGA GAGTGGTCGA GCAGCATGCG TGAATGCCGT AAGGCAGAAG 
AAGCGTGCCA CTGTGGACTC CATCAAGGGT CAGTTTGGTT TTCTTAACTT TGAGGTGGAG 
GACGGCAAAA AACTGTTCTT CCACATGTCT GAGGTGCAGG GCAATACTGT CGCTTTACAT 
CCAGGCGATA CCGTGGAGTT CTCCGTGGTC ACCAATCAGC GCAATGGAAA GTCGTCGGCT 
TGCAATGTTC TGAAAATAAA CGATCGTCCG GATCGTCTGA TCTCGCGCCT TAAGCTCAAC 
GGCGATGACA CTGTGCCGCG ACTGATCCTC ATTCGCGCAC CCAAGGGACC GCAGGGCAAG 
GGCTTTTCCG TTCTAGCTCG TCATCCACGC ATTCCAGGCA ACTTGGTGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unr ( NM_001144440.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137912.1 Drosophila melanogaster upstream of N-ras, transcript variant B (Unr), mRNA.

Price & Availability↑

CloneID	OFa17891
Clone ID Related Accession (Same CDS sequence)	NM_001274650.1 , NM_001300054.1 , NM_001144440.2
Accession Version	NM_001144440.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	upstream of N-ras, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_001144440.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATACCC AATCGAAGGT GTACCGTACC GGCGAAGAAA TCTATGACAA CTTGCCATGC 
GATAGTTATT TCAACATGAA TGCCATTCGC AACCTGGGAA TCCCAACAAC GTTTCCGACC 
ATTGGAACAT TTACACTTGA CTCTACTACG TTGGGACTGC AGCCGCAGGG TCAGGGCCCG 
TCGCCCCAGC AGCAGCAGCA TCAACAGCAA CAACAACAGC AGCAGCAGCA ACATCAGCAG 
AATATGCACC ACCATCAACA TCAGCAGCAA CACATGCAGC AGCAGCAGCA ACAACAACAA 
CATCAGCAGC AACACCAACA GCAGCAACAT CAACAACAAC AGCAACATCA GCAGCAGCAG 
CAGCAGCAAC AACAGCATCC AACCATTGGA ATGTTCGATG CCAACGAGGT TAACGATGTC 
ATCCAGAATC CTCCTCAAAT CGGGGTATTC CAGTCTAACA GCGTTCTGAC CAACGGTGCC 
GGCAGTGGCT CCTCGATCTT TGGCTCCCAA TCGAGCAATT CATCGGCGGC AGCTGCAGAT 
CCCAGCCAGA CCACCCGGGA AACTGGCATC ATTGAAAAGT TGTTGCATTC GTATGGATTC 
ATTCAGTGCT GTGAGCGGCA GGCGCGTCTT TTCTTTCATT TCTCGCAATT CAGCGGTAAT 
ATTGATCATT TGAAAATCGG AGATCCCGTG GAGTTTGAGA TGACCTATGA TCGCCGCACC 
GGCAAGCCGA TAGCTAGTCA AGTATCAAAA ATAGCCCCAG AGGTTGTTCT CTCCGAGGAG 
CGGGTCACCG GCACGGTGAC CACCGAGCTG CGCACCGATA GCGCCAACAA TGTGCTTAAC 
TCCAGCGAGA CCACAGGTCG CATAAGCTAT GAGAACCGAG GCGAATGCTT CTTTTTACCC 
TATACCAAAG ACGATGTGGA AGGTAATGTC AACTTGCGCG CTGGCGACAA GGTCAGCTTT 
CAAATCGCAA CGAACCAAAG AGGTAATCTA GGCGCCTGCC ATATTCGCCT GGAAAATCCG 
GCCCAGCCGG TTAAGTATCG TGGGGTTGTC TGCTCGATGA AGGAGTCGTT TGGCTTTATC 
GAACGTGCCG ATGTGGTCAA GGAGATTTTC TTCCACTTCT CAGAGGCTGA GGGCAATGTT 
GAGCTGCGTC CCGGTGACGA TGTCGAGTTC ACCATCCAGA CGCGGAGCGT AAGTGGCAGT 
GGCGATCCGA CCAATCCCAA TATGCTCCAA TCAAAAATGC AGTCTGCATC TGTGCCTCCG 
CAGGGCCGTG AATTTGCGTG CAACATCACG CGCCTAGCTC CGGGATCAGT GATCTTTGAG 
GACGTCGATA GCACGGTGTA TAAGGGCCAG GTGCTTAAGT CGCTTGATCG CAACAACCCG 
GTGCGCCAGA ACAATGATCC CTTGCCGGGT CGTATTCGTT ATAGGGCCTT GGATTACTCC 
GAAGTGGAGG TTCCTTTCGG GGACAAGGAT CAAAAGGGTG ACTTCACCCT GCGCCACGGC 
GATTGGGTGC AGTTCCTGCT TGCCACCGAT CGGCGTGATC AATTGCAGAG GGCCACCTCC 
ATTGCCCTGT TGGACGAGAC TTTCAAGGTT TCCGGCGAGA AGCGGGAGCA GGGCACCATT 
GCCTCTCTCA AGGAGGGCTT CGGATTCCTG CGCTGCGTAG AGCGTCAGGC TCGTTTATTT 
TTCCACTTTA CTGAAGTTTT AGATACGAGC CGCGAAATCG ATATCAATGA TGAGGTAGAA 
TTCACTGTCA TTCAGGAGCC TGGATTGGCC TATAATAACT CGCGTTTGCA GGCCATTCGC 
ATTAAACACT TGCCGCCCAA CTCAGTGCAA TTTGAAACTC TAGTGGCCAG CAACATAGAA 
GGATGTGTAA CTCGCGAGGC GCCTAAGAGC CCAATTAAAT CTCAAGATCG CGTCGAGGGC 
GGTGTGATTA CCTATGAACA TGCCGATGTT AAAAAGACTA TAATGTATTT TCTTAAAGAC 
TGCGAAAAAC CACCAAGGAT TGGGGAGCGC GTACGCTTCG ATATTTACAT GGTCAAACGT 
AACAAGGAGT GTATAGCCGT CAACGTACAG CAAGTATCCC TGCATCAGCA GCAACAACAG 
CAGCAACAGC AGTTGCATCT TAATCAGTCG AATGCTGGGG CAAATATCAA CCAAAATGAC 
CAGCTGGGAG GATTGTCGAA TGGAATCAGT AGCAGCAGCT CGAATGCTTC GCTACAGAAT 
GGCTATGTCA TGCACGGCAG CCCTGGCGGC AGCACCAGCA GCGTGGGCAG CAACAATCCG 
GTTCATCTGG ATGAATTCAA GATGGAGAAC AACAACCATG CCGGATCCGA TGCCGGTCAG 
GTGTACCGTG GCTTCATAGC CGTTATGAAG GAGAACTTTG GATTCATTGA GACTTTGTCC 
CACGATGAGG AGGTCTTCTT TCACTTTAGC AACTATATGG GCAATCCCAA TTGGTTGGAG 
CTGGGTCAGG AGGTGGAGTA CACTCTTGCT CGTAATGGGA ATACATCCGT TTCTGGAAAC 
TGTCTGCCTG CGGAAAATGT CCGTATGCTG CCAAAGAACT CTATTCCCCA GCCGGCTGTG 
CTGGAAACCA CACACAATGG CGTGGTAGCA CGTCCACTGC GTTGCATCAA TCCCGACCAG 
CAGGAATATG CCGGTCTCAT TGAAATTCTC GATGAGCTAC GTACCACTGT CATTTCGCAA 
CACGAATTCG GGATTACTAG TCTGGTGAAC AAGAGGGATC TGCTCCAGAA GGGCGATCTC 
GTTAGCTTCC GCATCGATGA GAGTGGTCGA GCAGCATGCG TGAATGCCGT AAGGCAGAAG 
AAGCGTGCCA CTGTGGACTC CATCAAGGGT CAGTTTGGTT TTCTTAACTT TGAGGTGGAG 
GACGGCAAAA AACTGTTCTT CCACATGTCT GAGGTGCAGG GCAATACTGT CGCTTTACAT 
CCAGGCGATA CCGTGGAGTT CTCCGTGGTC ACCAATCAGC GCAATGGAAA GTCGTCGGCT 
TGCAATGTTC TGAAAATAAA CGATCGTCCG GATCGTCTGA TCTCGCGCCT TAAGCTCAAC 
GGCGATGACA CTGTGCCGCG ACTGATCCTC ATTCGCGCAC CCAAGGGACC GCAGGGCAAG 
GGCTTTTCCG TTCTAGCTCG TCATCCACGC ATTCCAGGCA ACTTGGTGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137912.1
CDS	3479..6652
Translation	MNTQSKVYRTGEEIYDNLPCDSYFNMNAIRNLGIPTTFPTIGTFTLDSTTLGLQPQGQGPSPQQQQHQQQQQQQQQQHQQNMHHHQHQQQHMQQQQQQQQHQQQHQQQQHQQQQQHQQQQQQQQQHPTIGMFDANEVNDVIQNPPQIGVFQSNSVLTNGAGSGSSIFGSQSSNSSAAAADPSQTTRETGIIEKLLHSYGFIQCCERQARLFFHFSQFSGNIDHLKIGDPVEFEMTYDRRTGKPIASQVSKIAPEVVLSEERVTGTVTTELRTDSANNVLNSSETTGRISYENRGECFFLPYTKDDVEGNVNLRAGDKVSFQIATNQRGNLGACHIRLENPAQPVKYRGVVCSMKESFGFIERADVVKEIFFHFSEAEGNVELRPGDDVEFTIQTRSVSGSGDPTNPNMLQSKMQSASVPPQGREFACNITRLAPGSVIFEDVDSTVYKGQVLKSLDRNNPVRQNNDPLPGRIRYRALDYSEVEVPFGDKDQKGDFTLRHGDWVQFLLATDRRDQLQRATSIALLDETFKVSGEKREQGTIASLKEGFGFLRCVERQARLFFHFTEVLDTSREIDINDEVEFTVIQEPGLAYNNSRLQAIRIKHLPPNSVQFETLVASNIEGCVTREAPKSPIKSQDRVEGGVITYEHADVKKTIMYFLKDCEKPPRIGERVRFDIYMVKRNKECIAVNVQQVSLHQQQQQQQQQLHLNQSNAGANINQNDQLGGLSNGISSSSSNASLQNGYVMHGSPGGSTSSVGSNNPVHLDEFKMENNNHAGSDAGQVYRGFIAVMKENFGFIETLSHDEEVFFHFSNYMGNPNWLELGQEVEYTLARNGNTSVSGNCLPAENVRMLPKNSIPQPAVLETTHNGVVARPLRCINPDQQEYAGLIEILDELRTTVISQHEFGITSLVNKRDLLQKGDLVSFRIDESGRAACVNAVRQKKRATVDSIKGQFGFLNFEVEDGKKLFFHMSEVQGNTVALHPGDTVEFSVVTNQRNGKSSACNVLKINDRPDRLISRLKLNGDDTVPRLILIRAPKGPQGKGFSVLARHPRIPGNLVE

Target ORF information:

RefSeq Version	NM_001144440.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster upstream of N-ras, transcript variant B (Unr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144440.2

ORF Insert Sequence:

ATGAATACCC AATCGAAGGT GTACCGTACC GGCGAAGAAA TCTATGACAA CTTGCCATGC 
GATAGTTATT TCAACATGAA TGCCATTCGC AACCTGGGAA TCCCAACAAC GTTTCCGACC 
ATTGGAACAT TTACACTTGA CTCTACTACG TTGGGACTGC AGCCGCAGGG TCAGGGCCCG 
TCGCCCCAGC AGCAGCAGCA TCAACAGCAA CAACAACAGC AGCAGCAGCA ACATCAGCAG 
AATATGCACC ACCATCAACA TCAGCAGCAA CACATGCAGC AGCAGCAGCA ACAACAACAA 
CATCAGCAGC AACACCAACA GCAGCAACAT CAACAACAAC AGCAACATCA GCAGCAGCAG 
CAGCAGCAAC AACAGCATCC AACCATTGGA ATGTTCGATG CCAACGAGGT TAACGATGTC 
ATCCAGAATC CTCCTCAAAT CGGGGTATTC CAGTCTAACA GCGTTCTGAC CAACGGTGCC 
GGCAGTGGCT CCTCGATCTT TGGCTCCCAA TCGAGCAATT CATCGGCGGC AGCTGCAGAT 
CCCAGCCAGA CCACCCGGGA AACTGGCATC ATTGAAAAGT TGTTGCATTC GTATGGATTC 
ATTCAGTGCT GTGAGCGGCA GGCGCGTCTT TTCTTTCATT TCTCGCAATT CAGCGGTAAT 
ATTGATCATT TGAAAATCGG AGATCCCGTG GAGTTTGAGA TGACCTATGA TCGCCGCACC 
GGCAAGCCGA TAGCTAGTCA AGTATCAAAA ATAGCCCCAG AGGTTGTTCT CTCCGAGGAG 
CGGGTCACCG GCACGGTGAC CACCGAGCTG CGCACCGATA GCGCCAACAA TGTGCTTAAC 
TCCAGCGAGA CCACAGGTCG CATAAGCTAT GAGAACCGAG GCGAATGCTT CTTTTTACCC 
TATACCAAAG ACGATGTGGA AGGTAATGTC AACTTGCGCG CTGGCGACAA GGTCAGCTTT 
CAAATCGCAA CGAACCAAAG AGGTAATCTA GGCGCCTGCC ATATTCGCCT GGAAAATCCG 
GCCCAGCCGG TTAAGTATCG TGGGGTTGTC TGCTCGATGA AGGAGTCGTT TGGCTTTATC 
GAACGTGCCG ATGTGGTCAA GGAGATTTTC TTCCACTTCT CAGAGGCTGA GGGCAATGTT 
GAGCTGCGTC CCGGTGACGA TGTCGAGTTC ACCATCCAGA CGCGGAGCGT AAGTGGCAGT 
GGCGATCCGA CCAATCCCAA TATGCTCCAA TCAAAAATGC AGTCTGCATC TGTGCCTCCG 
CAGGGCCGTG AATTTGCGTG CAACATCACG CGCCTAGCTC CGGGATCAGT GATCTTTGAG 
GACGTCGATA GCACGGTGTA TAAGGGCCAG GTGCTTAAGT CGCTTGATCG CAACAACCCG 
GTGCGCCAGA ACAATGATCC CTTGCCGGGT CGTATTCGTT ATAGGGCCTT GGATTACTCC 
GAAGTGGAGG TTCCTTTCGG GGACAAGGAT CAAAAGGGTG ACTTCACCCT GCGCCACGGC 
GATTGGGTGC AGTTCCTGCT TGCCACCGAT CGGCGTGATC AATTGCAGAG GGCCACCTCC 
ATTGCCCTGT TGGACGAGAC TTTCAAGGTT TCCGGCGAGA AGCGGGAGCA GGGCACCATT 
GCCTCTCTCA AGGAGGGCTT CGGATTCCTG CGCTGCGTAG AGCGTCAGGC TCGTTTATTT 
TTCCACTTTA CTGAAGTTTT AGATACGAGC CGCGAAATCG ATATCAATGA TGAGGTAGAA 
TTCACTGTCA TTCAGGAGCC TGGATTGGCC TATAATAACT CGCGTTTGCA GGCCATTCGC 
ATTAAACACT TGCCGCCCAA CTCAGTGCAA TTTGAAACTC TAGTGGCCAG CAACATAGAA 
GGATGTGTAA CTCGCGAGGC GCCTAAGAGC CCAATTAAAT CTCAAGATCG CGTCGAGGGC 
GGTGTGATTA CCTATGAACA TGCCGATGTT AAAAAGACTA TAATGTATTT TCTTAAAGAC 
TGCGAAAAAC CACCAAGGAT TGGGGAGCGC GTACGCTTCG ATATTTACAT GGTCAAACGT 
AACAAGGAGT GTATAGCCGT CAACGTACAG CAAGTATCCC TGCATCAGCA GCAACAACAG 
CAGCAACAGC AGTTGCATCT TAATCAGTCG AATGCTGGGG CAAATATCAA CCAAAATGAC 
CAGCTGGGAG GATTGTCGAA TGGAATCAGT AGCAGCAGCT CGAATGCTTC GCTACAGAAT 
GGCTATGTCA TGCACGGCAG CCCTGGCGGC AGCACCAGCA GCGTGGGCAG CAACAATCCG 
GTTCATCTGG ATGAATTCAA GATGGAGAAC AACAACCATG CCGGATCCGA TGCCGGTCAG 
GTGTACCGTG GCTTCATAGC CGTTATGAAG GAGAACTTTG GATTCATTGA GACTTTGTCC 
CACGATGAGG AGGTCTTCTT TCACTTTAGC AACTATATGG GCAATCCCAA TTGGTTGGAG 
CTGGGTCAGG AGGTGGAGTA CACTCTTGCT CGTAATGGGA ATACATCCGT TTCTGGAAAC 
TGTCTGCCTG CGGAAAATGT CCGTATGCTG CCAAAGAACT CTATTCCCCA GCCGGCTGTG 
CTGGAAACCA CACACAATGG CGTGGTAGCA CGTCCACTGC GTTGCATCAA TCCCGACCAG 
CAGGAATATG CCGGTCTCAT TGAAATTCTC GATGAGCTAC GTACCACTGT CATTTCGCAA 
CACGAATTCG GGATTACTAG TCTGGTGAAC AAGAGGGATC TGCTCCAGAA GGGCGATCTC 
GTTAGCTTCC GCATCGATGA GAGTGGTCGA GCAGCATGCG TGAATGCCGT AAGGCAGAAG 
AAGCGTGCCA CTGTGGACTC CATCAAGGGT CAGTTTGGTT TTCTTAACTT TGAGGTGGAG 
GACGGCAAAA AACTGTTCTT CCACATGTCT GAGGTGCAGG GCAATACTGT CGCTTTACAT 
CCAGGCGATA CCGTGGAGTT CTCCGTGGTC ACCAATCAGC GCAATGGAAA GTCGTCGGCT 
TGCAATGTTC TGAAAATAAA CGATCGTCCG GATCGTCTGA TCTCGCGCCT TAAGCTCAAC 
GGCGATGACA CTGTGCCGCG ACTGATCCTC ATTCGCGCAC CCAAGGGACC GCAGGGCAAG 
GGCTTTTCCG TTCTAGCTCG TCATCCACGC ATTCCAGGCA ACTTGGTGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unr ( NM_139969.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648226.2 Drosophila melanogaster upstream of N-ras, transcript variant A (Unr), mRNA.

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CloneID	OFa20734
Clone ID Related Accession (Same CDS sequence)	NM_139969.4
Accession Version	NM_139969.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	upstream of N-ras, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139969.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATACCC AATCGAAGGT GTACCGTACC GGCGAAGAAA TCTATGACAA CTTGCCATGC 
GATAGTTATT TCAACATGAA TGCCATTCGC AACCTGGGAA TCCCAACAAC GTTTCCGACC 
ATTGGAACAT TTACACTTGA CTCTACTACG TTGGGACTGC AGCCGCAGGG TCAGGGCCCG 
TCGCCCCAGC AGCAGCAGCA TCAACAGCAA CAACAACAGC AGCAGCAGCA ACATCAGCAG 
AATATGCACC ACCATCAACA TCAGCAGCAA CACATGCAGC AGCAGCAGCA ACAACAACAA 
CATCAGCAGC AACACCAACA GCAGCAACAT CAACAACAAC AGCAACATCA GCAGCAGCAG 
CAGCAGCAAC AACAGCATCC AACCATTGGA ATGTTCGATG CCAACGAGGT TAACGATGTC 
ATCCAGAATC CTCCTCAAAT CGGGGTATTC CAGTCTAACA GCGTTCTGAC CAACGGTGCC 
GGCAGTGGCT CCTCGATCTT TGGCTCCCAA TCGAGCAATT CATCGGCGGC AGCTGCAGAT 
CCCAGCCAGA CCACCCGGGA AACTGGCATC ATTGAAAAGT TGTTGCATTC GTATGGATTC 
ATTCAGTGCT GTGAGCGGCA GGCGCGTCTT TTCTTTCATT TCTCGCAATT CAGCGGTAAT 
ATTGATCATT TGAAAATCGG AGATCCCGTG GAGTTTGAGA TGACCTATGA TCGCCGCACC 
GGCAAGCCGA TAGCTAGTCA AGTATCAAAA ATAGCCCCAG AGGTTGTTCT CTCCGAGGAG 
CGGGTCACCG GCACGGTGAC CACCGAGCTG CGCACCGATA GCGCCAACAA TGTGCTTAAC 
TCCAGCGAGA CCACAGGTCG CATAAGCTAT GAGAACCGAG GCGAATGCTT CTTTTTACCC 
TATACCAAAG ACGATGTGGA AGGTAATGTC AACTTGCGCG CTGGCGACAA GGTCAGCTTT 
CAAATCGCAA CGAACCAAAG AGGTAATCTA GGCGCCTGCC ATATTCGCCT GGAAAATCCG 
GCCCAGCCGG TTAAGTATCG TGGGGTTGTC TGCTCGATGA AGGAGTCGTT TGGCTTTATC 
GAACGTGCCG ATGTGGTCAA GGAGATTTTC TTCCACTTCT CAGAGGCTGA GGGCAATGTT 
GAGCTGCGTC CCGGTGACGA TGTCGAGTTC ACCATCCAGA CGCGGAGCTC TGCATCTGTG 
CCTCCGCAGG GCCGTGAATT TGCGTGCAAC ATCACGCGCC TAGCTCCGGG ATCAGTGATC 
TTTGAGGACG TCGATAGCAC GGTGTATAAG GGCCAGGTGC TTAAGTCGCT TGATCGCAAC 
AACCCGGTGC GCCAGAACAA TGATCCCTTG CCGGGTCGTA TTCGTTATAG GGCCTTGGAT 
TACTCCGAAG TGGAGGTTCC TTTCGGGGAC AAGGATCAAA AGGGTGACTT CACCCTGCGC 
CACGGCGATT GGGTGCAGTT CCTGCTTGCC ACCGATCGGC GTGATCAATT GCAGAGGGCC 
ACCTCCATTG CCCTGTTGGA CGAGACTTTC AAGGTTTCCG GCGAGAAGCG GGAGCAGGGC 
ACCATTGCCT CTCTCAAGGA GGGCTTCGGA TTCCTGCGCT GCGTAGAGCG TCAGGCTCGT 
TTATTTTTCC ACTTTACTGA AGTTTTAGAT ACGAGCCGCG AAATCGATAT CAATGATGAG 
GTAGAATTCA CTGTCATTCA GGAGCCTGGA TTGGCCTATA ATAACTCGCG TTTGCAGGCC 
ATTCGCATTA AACACTTGCC GCCCAACTCA GTGCAATTTG AAACTCTAGT GGCCAGCAAC 
ATAGAAGGAT GTGTAACTCG CGAGGCGCCT AAGAGCCCAA TTAAATCTCA AGATCGCGTC 
GAGGGCGGTG TGATTACCTA TGAACATGCC GATGTTAAAA AGACTATAAT GTATTTTCTT 
AAAGACTGCG AAAAACCACC AAGGATTGGG GAGCGCGTAC GCTTCGATAT TTACATGGTC 
AAACGTAACA AGGAGTGTAT AGCCGTCAAC GTACAGCAAG TATCCCTGCA TCAGCAGCAA 
CAACAGCAGC AACAGCAGTT GCATCTTAAT CAGTCGAATG CTGGGGCAAA TATCAACCAA 
AATGACCAGC TGGGAGGATT GTCGAATGGA ATCAGTAGCA GCAGCTCGAA TGCTTCGCTA 
CAGAATGGCT ATGTCATGCA CGGCAGCCCT GGCGGCAGCA CCAGCAGCGT GGGCAGCAAC 
AATCCGGTTC ATCTGGATGA ATTCAAGATG GAGAACAACA ACCATGCCGG ATCCGATGCC 
GGTCAGGTGT ACCGTGGCTT CATAGCCGTT ATGAAGGAGA ACTTTGGATT CATTGAGACT 
TTGTCCCACG ATGAGGAGGT CTTCTTTCAC TTTAGCAACT ATATGGGCAA TCCCAATTGG 
TTGGAGCTGG GTCAGGAGGT GGAGTACACT CTTGCTCGTA ATGGGAATAC ATCCGTTTCT 
GGAAACTGTC TGCCTGCGGA AAATGTCCGT ATGCTGCCAA AGAACTCTAT TCCCCAGCCG 
GCTGTGCTGG AAACCACACA CAATGGCGTG GTAGCACGTC CACTGCGTTG CATCAATCCC 
GACCAGCAGG AATATGCCGG TCTCATTGAA ATTCTCGATG AGCTACGTAC CACTGTCATT 
TCGCAACACG AATTCGGGAT TACTAGTCTG GTGAACAAGA GGGATCTGCT CCAGAAGGGC 
GATCTCGTTA GCTTCCGCAT CGATGAGAGT GGTCGAGCAG CATGCGTGAA TGCCGTAAGG 
CAGAAGAAGC GTGCCACTGT GGACTCCATC AAGGGTCAGT TTGGTTTTCT TAACTTTGAG 
GTGGAGGACG GCAAAAAACT GTTCTTCCAC ATGTCTGAGG TGCAGGGCAA TACTGTCGCT 
TTACATCCAG GCGATACCGT GGAGTTCTCC GTGGTCACCA ATCAGCGCAA TGGAAAGTCG 
TCGGCTTGCA ATGTTCTGAA AATAAACGAT CGTCCGGATC GTCTGATCTC GCGCCTTAAG 
CTCAACGGCG ATGACACTGT GCCGCGACTG ATCCTCATTC GCGCACCCAA GGGACCGCAG 
GGCAAGGGCT TTTCCGTTCT AGCTCGTCAT CCACGCATTC CAGGCAACTT GGTGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648226.2
CDS	3539..6658
Translation	MNTQSKVYRTGEEIYDNLPCDSYFNMNAIRNLGIPTTFPTIGTFTLDSTTLGLQPQGQGPSPQQQQHQQQQQQQQQQHQQNMHHHQHQQQHMQQQQQQQQHQQQHQQQQHQQQQQHQQQQQQQQQHPTIGMFDANEVNDVIQNPPQIGVFQSNSVLTNGAGSGSSIFGSQSSNSSAAAADPSQTTRETGIIEKLLHSYGFIQCCERQARLFFHFSQFSGNIDHLKIGDPVEFEMTYDRRTGKPIASQVSKIAPEVVLSEERVTGTVTTELRTDSANNVLNSSETTGRISYENRGECFFLPYTKDDVEGNVNLRAGDKVSFQIATNQRGNLGACHIRLENPAQPVKYRGVVCSMKESFGFIERADVVKEIFFHFSEAEGNVELRPGDDVEFTIQTRSSASVPPQGREFACNITRLAPGSVIFEDVDSTVYKGQVLKSLDRNNPVRQNNDPLPGRIRYRALDYSEVEVPFGDKDQKGDFTLRHGDWVQFLLATDRRDQLQRATSIALLDETFKVSGEKREQGTIASLKEGFGFLRCVERQARLFFHFTEVLDTSREIDINDEVEFTVIQEPGLAYNNSRLQAIRIKHLPPNSVQFETLVASNIEGCVTREAPKSPIKSQDRVEGGVITYEHADVKKTIMYFLKDCEKPPRIGERVRFDIYMVKRNKECIAVNVQQVSLHQQQQQQQQQLHLNQSNAGANINQNDQLGGLSNGISSSSSNASLQNGYVMHGSPGGSTSSVGSNNPVHLDEFKMENNNHAGSDAGQVYRGFIAVMKENFGFIETLSHDEEVFFHFSNYMGNPNWLELGQEVEYTLARNGNTSVSGNCLPAENVRMLPKNSIPQPAVLETTHNGVVARPLRCINPDQQEYAGLIEILDELRTTVISQHEFGITSLVNKRDLLQKGDLVSFRIDESGRAACVNAVRQKKRATVDSIKGQFGFLNFEVEDGKKLFFHMSEVQGNTVALHPGDTVEFSVVTNQRNGKSSACNVLKINDRPDRLISRLKLNGDDTVPRLILIRAPKGPQGKGFSVLARHPRIPGNLVE

Target ORF information:

RefSeq Version	NM_139969.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster upstream of N-ras, transcript variant A (Unr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139969.4

ORF Insert Sequence:

ATGAATACCC AATCGAAGGT GTACCGTACC GGCGAAGAAA TCTATGACAA CTTGCCATGC 
GATAGTTATT TCAACATGAA TGCCATTCGC AACCTGGGAA TCCCAACAAC GTTTCCGACC 
ATTGGAACAT TTACACTTGA CTCTACTACG TTGGGACTGC AGCCGCAGGG TCAGGGCCCG 
TCGCCCCAGC AGCAGCAGCA TCAACAGCAA CAACAACAGC AGCAGCAGCA ACATCAGCAG 
AATATGCACC ACCATCAACA TCAGCAGCAA CACATGCAGC AGCAGCAGCA ACAACAACAA 
CATCAGCAGC AACACCAACA GCAGCAACAT CAACAACAAC AGCAACATCA GCAGCAGCAG 
CAGCAGCAAC AACAGCATCC AACCATTGGA ATGTTCGATG CCAACGAGGT TAACGATGTC 
ATCCAGAATC CTCCTCAAAT CGGGGTATTC CAGTCTAACA GCGTTCTGAC CAACGGTGCC 
GGCAGTGGCT CCTCGATCTT TGGCTCCCAA TCGAGCAATT CATCGGCGGC AGCTGCAGAT 
CCCAGCCAGA CCACCCGGGA AACTGGCATC ATTGAAAAGT TGTTGCATTC GTATGGATTC 
ATTCAGTGCT GTGAGCGGCA GGCGCGTCTT TTCTTTCATT TCTCGCAATT CAGCGGTAAT 
ATTGATCATT TGAAAATCGG AGATCCCGTG GAGTTTGAGA TGACCTATGA TCGCCGCACC 
GGCAAGCCGA TAGCTAGTCA AGTATCAAAA ATAGCCCCAG AGGTTGTTCT CTCCGAGGAG 
CGGGTCACCG GCACGGTGAC CACCGAGCTG CGCACCGATA GCGCCAACAA TGTGCTTAAC 
TCCAGCGAGA CCACAGGTCG CATAAGCTAT GAGAACCGAG GCGAATGCTT CTTTTTACCC 
TATACCAAAG ACGATGTGGA AGGTAATGTC AACTTGCGCG CTGGCGACAA GGTCAGCTTT 
CAAATCGCAA CGAACCAAAG AGGTAATCTA GGCGCCTGCC ATATTCGCCT GGAAAATCCG 
GCCCAGCCGG TTAAGTATCG TGGGGTTGTC TGCTCGATGA AGGAGTCGTT TGGCTTTATC 
GAACGTGCCG ATGTGGTCAA GGAGATTTTC TTCCACTTCT CAGAGGCTGA GGGCAATGTT 
GAGCTGCGTC CCGGTGACGA TGTCGAGTTC ACCATCCAGA CGCGGAGCTC TGCATCTGTG 
CCTCCGCAGG GCCGTGAATT TGCGTGCAAC ATCACGCGCC TAGCTCCGGG ATCAGTGATC 
TTTGAGGACG TCGATAGCAC GGTGTATAAG GGCCAGGTGC TTAAGTCGCT TGATCGCAAC 
AACCCGGTGC GCCAGAACAA TGATCCCTTG CCGGGTCGTA TTCGTTATAG GGCCTTGGAT 
TACTCCGAAG TGGAGGTTCC TTTCGGGGAC AAGGATCAAA AGGGTGACTT CACCCTGCGC 
CACGGCGATT GGGTGCAGTT CCTGCTTGCC ACCGATCGGC GTGATCAATT GCAGAGGGCC 
ACCTCCATTG CCCTGTTGGA CGAGACTTTC AAGGTTTCCG GCGAGAAGCG GGAGCAGGGC 
ACCATTGCCT CTCTCAAGGA GGGCTTCGGA TTCCTGCGCT GCGTAGAGCG TCAGGCTCGT 
TTATTTTTCC ACTTTACTGA AGTTTTAGAT ACGAGCCGCG AAATCGATAT CAATGATGAG 
GTAGAATTCA CTGTCATTCA GGAGCCTGGA TTGGCCTATA ATAACTCGCG TTTGCAGGCC 
ATTCGCATTA AACACTTGCC GCCCAACTCA GTGCAATTTG AAACTCTAGT GGCCAGCAAC 
ATAGAAGGAT GTGTAACTCG CGAGGCGCCT AAGAGCCCAA TTAAATCTCA AGATCGCGTC 
GAGGGCGGTG TGATTACCTA TGAACATGCC GATGTTAAAA AGACTATAAT GTATTTTCTT 
AAAGACTGCG AAAAACCACC AAGGATTGGG GAGCGCGTAC GCTTCGATAT TTACATGGTC 
AAACGTAACA AGGAGTGTAT AGCCGTCAAC GTACAGCAAG TATCCCTGCA TCAGCAGCAA 
CAACAGCAGC AACAGCAGTT GCATCTTAAT CAGTCGAATG CTGGGGCAAA TATCAACCAA 
AATGACCAGC TGGGAGGATT GTCGAATGGA ATCAGTAGCA GCAGCTCGAA TGCTTCGCTA 
CAGAATGGCT ATGTCATGCA CGGCAGCCCT GGCGGCAGCA CCAGCAGCGT GGGCAGCAAC 
AATCCGGTTC ATCTGGATGA ATTCAAGATG GAGAACAACA ACCATGCCGG ATCCGATGCC 
GGTCAGGTGT ACCGTGGCTT CATAGCCGTT ATGAAGGAGA ACTTTGGATT CATTGAGACT 
TTGTCCCACG ATGAGGAGGT CTTCTTTCAC TTTAGCAACT ATATGGGCAA TCCCAATTGG 
TTGGAGCTGG GTCAGGAGGT GGAGTACACT CTTGCTCGTA ATGGGAATAC ATCCGTTTCT 
GGAAACTGTC TGCCTGCGGA AAATGTCCGT ATGCTGCCAA AGAACTCTAT TCCCCAGCCG 
GCTGTGCTGG AAACCACACA CAATGGCGTG GTAGCACGTC CACTGCGTTG CATCAATCCC 
GACCAGCAGG AATATGCCGG TCTCATTGAA ATTCTCGATG AGCTACGTAC CACTGTCATT 
TCGCAACACG AATTCGGGAT TACTAGTCTG GTGAACAAGA GGGATCTGCT CCAGAAGGGC 
GATCTCGTTA GCTTCCGCAT CGATGAGAGT GGTCGAGCAG CATGCGTGAA TGCCGTAAGG 
CAGAAGAAGC GTGCCACTGT GGACTCCATC AAGGGTCAGT TTGGTTTTCT TAACTTTGAG 
GTGGAGGACG GCAAAAAACT GTTCTTCCAC ATGTCTGAGG TGCAGGGCAA TACTGTCGCT 
TTACATCCAG GCGATACCGT GGAGTTCTCC GTGGTCACCA ATCAGCGCAA TGGAAAGTCG 
TCGGCTTGCA ATGTTCTGAA AATAAACGAT CGTCCGGATC GTCTGATCTC GCGCCTTAAG 
CTCAACGGCG ATGACACTGT GCCGCGACTG ATCCTCATTC GCGCACCCAA GGGACCGCAG 
GGCAAGGGCT TTTCCGTTCT AGCTCGTCAT CCACGCATTC CAGGCAACTT GGTGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Unr cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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