Atg18a cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atg18a
Entrez Gene ID	38913
Full Name	Autophagy-related 18a
Synonyms	ATG18,ATG18.1,Atg18,CG7986,Dmel\CG7986,atg18
General protein information	Preferred Names Autophagy-related 18a Names CG7986 gene product from transcript CG7986-RG Atg18a-PA Atg18a-PB Atg18a-PC Atg18a-PD Atg18a-PE Atg18a-PF Atg18a-PG Autophagy-specific gene 18 Autophagy-specific gene 18a CG7986-PA CG7986-PB CG7986-PC CG7986-PD CG7986-PE CG7986-PF CG7986-PG
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66B11-66B11

mRNA and Protein(s)

mRNA	Protein	Name
NM_168259.3	NP_729341.1	Autophagy-related 18a, isoform B
NM_001300048.1	NP_001286977.1	Autophagy-related 18a, isoform G
NM_001274637.1	NP_001261566.1	Autophagy-related 18a, isoform F
NM_001274635.1	NP_001261564.1	Autophagy-related 18a, isoform D
NM_139927.4	NP_648184.1	Autophagy-related 18a, isoform A
NM_206301.4	NP_996023.1	Autophagy-related 18a, isoform C
NM_001274636.1	NP_001261565.1	Autophagy-related 18a, isoform E

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	WIPI2	XP_850630.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005910	XP_315940.3
Eremothecium gossypii	AGOS_AAL119W	NP_982423.1
Pan troglodytes (chimpanzee)	WIPI2	XP_003318335.1
Bos taurus (cattle)	WIPI2	NP_001039594.1
Drosophila melanogaster (fruit fly)	Atg18	NP_001261565.1
Kluyveromyces lactis	KLLA0D04664g	XP_453268.1
Schizosaccharomyces pombe (fission yeast)	atg18	NP_594055.1
Magnaporthe oryzae (rice blast fungus)	MGG_03139	XP_003716858.1
Mus musculus (house mouse)	Wipi2	NP_848485.1
Rattus norvegicus (Norway rat)	Wipi2	NP_001007616.1
Gallus gallus (chicken)	WIPI2	NP_001006162.1
Neurospora crassa	NCU03441	XP_956727.1
Oryza sativa (rice)	Os02g0791800	NP_001048361.1
Homo sapiens (human)	WIPI2	NP_057087.2
Saccharomyces cerevisiae (baker's yeast)	ATG18	NP_444297.1
Arabidopsis thaliana (thale cress)	G18B	NP_194780.2
Xenopus tropicalis (tropical clawed frog)	LOC100497399	XP_004917971.1
Caenorhabditis elegans (roundworm)	atg-18	NP_741576.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The simultaneous interaction of Atg18 with both Atg9 and Ref(2)P raises the possibility that Atg18 may facilitate selective degradation of ubiquitinated protein aggregates by autophagy.
Title: Different effects of Atg2 and Atg18 mutations on Atg8a and Atg9 trafficking during starvation in Drosophila.

The following Atg18a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atg18a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17879	NM_139927.4 Latest version!	Drosophila melanogaster Autophagy-related 18a (Atg18a), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa17879	NM_001300048.1 Latest version!	Drosophila melanogaster Autophagy-related 18a (Atg18a), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa17879	NM_206301.4 Latest version!	Drosophila melanogaster Autophagy-related 18a (Atg18a), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa17879	NM_168259.3 Latest version!	Drosophila melanogaster Autophagy-related 18a (Atg18a), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa17880	NM_001274635.1 Latest version!	Drosophila melanogaster Autophagy-related 18a (Atg18a), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa20695	NM_001274636.1 Latest version!	Drosophila melanogaster Autophagy-related 18a (Atg18a), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa20696	NM_001274637.1 Latest version!	Drosophila melanogaster Autophagy-related 18a (Atg18a), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atg18a ( NM_168259.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729341.1 Drosophila melanogaster Autophagy-related 18a (Atg18a), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17879
Clone ID Related Accession (Same CDS sequence)	NM_168259.3 , NM_001300048.1 , NM_139927.4 , NM_206301.4
Accession Version	NM_168259.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Autophagy-related 18a, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168259.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGCC TGCTCGGGCG CACCGATGTG GATGCGGGCG AAGTGTTCGT CAACTTCAAC 
CAGAACATAA CTTCCCTGGC CGTGGCCACC AGTGGAGGCT ACAGCCTGTA CAGCCTGGGC 
TCCGTGGACT CGACCCTGGA CAAGATCTAC CACACGAAAA GCGACGAGCT GTTCCTCATC 
GAGCGTCTGT TCGAGAGCTC CTTGGTGGCA ATAGTTTCCC AGCGAGCACC ACGCAAGCTC 
AAGGTGTGCC ACTTTAAGAA GCAGAGCGAG ATCTGCAACT ACTCGTATGC AAACACAATA 
CTGGCCGTGA AGCTCAATCG CGAGCGATTG ATTGTGTGCC TGGAGGAGTC ATTGTATATC 
CACAATATCC AGGACATGAA GGTGGTGCAC ACCATACGCG ACACACCGTG CAATCCGCAG 
GGTCTTTGTG CCCTCTCCTC GTCGTCGGAG CACTGCTATC TGGCCTACCC AGGCAGCGTG 
ACTGCTGGCG AGGTACAGAT CTTTGATGCC ATCAACTTGC ACGCCAAGAC CATGATTCCC 
GCCCACGACA CACCTCTGGC GGCATTGGCC TTTAGTCCCT CGGGCACGGA GATAGCCACG 
GCCAGTGAGC GTGGCACGGT GATCCGAGTC TTCAGCTCCC AGGATGGCAG TCGACTCTTT 
GAACTGCGAC GGGGACTAAA GCGCTGTGTG TCCATCGTTT CGCTGTCGTT TAGCACGTGC 
GCCGAATATC TGGTGTCCAG CTCCAACACC GAGACGGTTC ACATCTTCCG GCTGGATCGC 
AGTGCCACGG AAACAGCCGA AGGCCATGGC AGCAAGCAGT CGAGCGACGA CTGGATGGGT 
TACTCGTTTT TTAGATTCTT AAGCAAAACG GTCACCAGCT ACCTGCCCAC GCAGGTGACC 
GACGTGTTTA GCCAAGGCCG TGCCTTCGCC TCGGTCACTC TGCCGGAGGC GGGAGTGCGC 
AGGATGTGCG CTATTGCCAC GATACAAAAG CAGCTAAGAC TTTTGATTGC CTCGCAGGAT 
GGGTACTTGT ACGTGTATTC CATTCCCACC GTCGAGGGCG CAGAGTGCCA GCTAATAAAG 
AGGCACGACC TGCGGCTGGA GGACCACTAT GCCATGGATA TCAAAGGTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729341.1
CDS	512..1645
Translation	MMSLLGRTDVDAGEVFVNFNQNITSLAVATSGGYSLYSLGSVDSTLDKIYHTKSDELFLIERLFESSLVAIVSQRAPRKLKVCHFKKQSEICNYSYANTILAVKLNRERLIVCLEESLYIHNIQDMKVVHTIRDTPCNPQGLCALSSSSEHCYLAYPGSVTAGEVQIFDAINLHAKTMIPAHDTPLAALAFSPSGTEIATASERGTVIRVFSSQDGSRLFELRRGLKRCVSIVSLSFSTCAEYLVSSSNTETVHIFRLDRSATETAEGHGSKQSSDDWMGYSFFRFLSKTVTSYLPTQVTDVFSQGRAFASVTLPEAGVRRMCAIATIQKQLRLLIASQDGYLYVYSIPTVEGAECQLIKRHDLRLEDHYAMDIKGA

Target ORF information:

RefSeq Version	NM_168259.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Autophagy-related 18a (Atg18a), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168259.3

ORF Insert Sequence:

ATGATGAGCC TGCTCGGGCG CACCGATGTG GATGCGGGCG AAGTGTTCGT CAACTTCAAC 
CAGAACATAA CTTCCCTGGC CGTGGCCACC AGTGGAGGCT ACAGCCTGTA CAGCCTGGGC 
TCCGTGGACT CGACCCTGGA CAAGATCTAC CACACGAAAA GCGACGAGCT GTTCCTCATC 
GAGCGTCTGT TCGAGAGCTC CTTGGTGGCA ATAGTTTCCC AGCGAGCACC ACGCAAGCTC 
AAGGTGTGCC ACTTTAAGAA GCAGAGCGAG ATCTGCAACT ACTCGTATGC AAACACAATA 
CTGGCCGTGA AGCTCAATCG CGAGCGATTG ATTGTGTGCC TGGAGGAGTC ATTGTATATC 
CACAATATCC AGGACATGAA GGTGGTGCAC ACCATACGCG ACACACCGTG CAATCCGCAG 
GGTCTTTGTG CCCTCTCCTC GTCGTCGGAG CACTGCTATC TGGCCTACCC AGGCAGCGTG 
ACTGCTGGCG AGGTACAGAT CTTTGATGCC ATCAACTTGC ACGCCAAGAC CATGATTCCC 
GCCCACGACA CACCTCTGGC GGCATTGGCC TTTAGTCCCT CGGGCACGGA GATAGCCACG 
GCCAGTGAGC GTGGCACGGT GATCCGAGTC TTCAGCTCCC AGGATGGCAG TCGACTCTTT 
GAACTGCGAC GGGGACTAAA GCGCTGTGTG TCCATCGTTT CGCTGTCGTT TAGCACGTGC 
GCCGAATATC TGGTGTCCAG CTCCAACACC GAGACGGTTC ACATCTTCCG GCTGGATCGC 
AGTGCCACGG AAACAGCCGA AGGCCATGGC AGCAAGCAGT CGAGCGACGA CTGGATGGGT 
TACTCGTTTT TTAGATTCTT AAGCAAAACG GTCACCAGCT ACCTGCCCAC GCAGGTGACC 
GACGTGTTTA GCCAAGGCCG TGCCTTCGCC TCGGTCACTC TGCCGGAGGC GGGAGTGCGC 
AGGATGTGCG CTATTGCCAC GATACAAAAG CAGCTAAGAC TTTTGATTGC CTCGCAGGAT 
GGGTACTTGT ACGTGTATTC CATTCCCACC GTCGAGGGCG CAGAGTGCCA GCTAATAAAG 
AGGCACGACC TGCGGCTGGA GGACCACTAT GCCATGGATA TCAAAGGTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atg18a ( NM_001300048.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286977.1 Drosophila melanogaster Autophagy-related 18a (Atg18a), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa17879
Clone ID Related Accession (Same CDS sequence)	NM_168259.3 , NM_001300048.1 , NM_139927.4 , NM_206301.4
Accession Version	NM_001300048.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Autophagy-related 18a, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGCC TGCTCGGGCG CACCGATGTG GATGCGGGCG AAGTGTTCGT CAACTTCAAC 
CAGAACATAA CTTCCCTGGC CGTGGCCACC AGTGGAGGCT ACAGCCTGTA CAGCCTGGGC 
TCCGTGGACT CGACCCTGGA CAAGATCTAC CACACGAAAA GCGACGAGCT GTTCCTCATC 
GAGCGTCTGT TCGAGAGCTC CTTGGTGGCA ATAGTTTCCC AGCGAGCACC ACGCAAGCTC 
AAGGTGTGCC ACTTTAAGAA GCAGAGCGAG ATCTGCAACT ACTCGTATGC AAACACAATA 
CTGGCCGTGA AGCTCAATCG CGAGCGATTG ATTGTGTGCC TGGAGGAGTC ATTGTATATC 
CACAATATCC AGGACATGAA GGTGGTGCAC ACCATACGCG ACACACCGTG CAATCCGCAG 
GGTCTTTGTG CCCTCTCCTC GTCGTCGGAG CACTGCTATC TGGCCTACCC AGGCAGCGTG 
ACTGCTGGCG AGGTACAGAT CTTTGATGCC ATCAACTTGC ACGCCAAGAC CATGATTCCC 
GCCCACGACA CACCTCTGGC GGCATTGGCC TTTAGTCCCT CGGGCACGGA GATAGCCACG 
GCCAGTGAGC GTGGCACGGT GATCCGAGTC TTCAGCTCCC AGGATGGCAG TCGACTCTTT 
GAACTGCGAC GGGGACTAAA GCGCTGTGTG TCCATCGTTT CGCTGTCGTT TAGCACGTGC 
GCCGAATATC TGGTGTCCAG CTCCAACACC GAGACGGTTC ACATCTTCCG GCTGGATCGC 
AGTGCCACGG AAACAGCCGA AGGCCATGGC AGCAAGCAGT CGAGCGACGA CTGGATGGGT 
TACTCGTTTT TTAGATTCTT AAGCAAAACG GTCACCAGCT ACCTGCCCAC GCAGGTGACC 
GACGTGTTTA GCCAAGGCCG TGCCTTCGCC TCGGTCACTC TGCCGGAGGC GGGAGTGCGC 
AGGATGTGCG CTATTGCCAC GATACAAAAG CAGCTAAGAC TTTTGATTGC CTCGCAGGAT 
GGGTACTTGT ACGTGTATTC CATTCCCACC GTCGAGGGCG CAGAGTGCCA GCTAATAAAG 
AGGCACGACC TGCGGCTGGA GGACCACTAT GCCATGGATA TCAAAGGTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286977.1
CDS	365..1498
Translation	MMSLLGRTDVDAGEVFVNFNQNITSLAVATSGGYSLYSLGSVDSTLDKIYHTKSDELFLIERLFESSLVAIVSQRAPRKLKVCHFKKQSEICNYSYANTILAVKLNRERLIVCLEESLYIHNIQDMKVVHTIRDTPCNPQGLCALSSSSEHCYLAYPGSVTAGEVQIFDAINLHAKTMIPAHDTPLAALAFSPSGTEIATASERGTVIRVFSSQDGSRLFELRRGLKRCVSIVSLSFSTCAEYLVSSSNTETVHIFRLDRSATETAEGHGSKQSSDDWMGYSFFRFLSKTVTSYLPTQVTDVFSQGRAFASVTLPEAGVRRMCAIATIQKQLRLLIASQDGYLYVYSIPTVEGAECQLIKRHDLRLEDHYAMDIKGA

Target ORF information:

RefSeq Version	NM_001300048.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Autophagy-related 18a (Atg18a), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300048.1

ORF Insert Sequence:

ATGATGAGCC TGCTCGGGCG CACCGATGTG GATGCGGGCG AAGTGTTCGT CAACTTCAAC 
CAGAACATAA CTTCCCTGGC CGTGGCCACC AGTGGAGGCT ACAGCCTGTA CAGCCTGGGC 
TCCGTGGACT CGACCCTGGA CAAGATCTAC CACACGAAAA GCGACGAGCT GTTCCTCATC 
GAGCGTCTGT TCGAGAGCTC CTTGGTGGCA ATAGTTTCCC AGCGAGCACC ACGCAAGCTC 
AAGGTGTGCC ACTTTAAGAA GCAGAGCGAG ATCTGCAACT ACTCGTATGC AAACACAATA 
CTGGCCGTGA AGCTCAATCG CGAGCGATTG ATTGTGTGCC TGGAGGAGTC ATTGTATATC 
CACAATATCC AGGACATGAA GGTGGTGCAC ACCATACGCG ACACACCGTG CAATCCGCAG 
GGTCTTTGTG CCCTCTCCTC GTCGTCGGAG CACTGCTATC TGGCCTACCC AGGCAGCGTG 
ACTGCTGGCG AGGTACAGAT CTTTGATGCC ATCAACTTGC ACGCCAAGAC CATGATTCCC 
GCCCACGACA CACCTCTGGC GGCATTGGCC TTTAGTCCCT CGGGCACGGA GATAGCCACG 
GCCAGTGAGC GTGGCACGGT GATCCGAGTC TTCAGCTCCC AGGATGGCAG TCGACTCTTT 
GAACTGCGAC GGGGACTAAA GCGCTGTGTG TCCATCGTTT CGCTGTCGTT TAGCACGTGC 
GCCGAATATC TGGTGTCCAG CTCCAACACC GAGACGGTTC ACATCTTCCG GCTGGATCGC 
AGTGCCACGG AAACAGCCGA AGGCCATGGC AGCAAGCAGT CGAGCGACGA CTGGATGGGT 
TACTCGTTTT TTAGATTCTT AAGCAAAACG GTCACCAGCT ACCTGCCCAC GCAGGTGACC 
GACGTGTTTA GCCAAGGCCG TGCCTTCGCC TCGGTCACTC TGCCGGAGGC GGGAGTGCGC 
AGGATGTGCG CTATTGCCAC GATACAAAAG CAGCTAAGAC TTTTGATTGC CTCGCAGGAT 
GGGTACTTGT ACGTGTATTC CATTCCCACC GTCGAGGGCG CAGAGTGCCA GCTAATAAAG 
AGGCACGACC TGCGGCTGGA GGACCACTAT GCCATGGATA TCAAAGGTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atg18a ( NM_139927.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648184.1 Drosophila melanogaster Autophagy-related 18a (Atg18a), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17879
Clone ID Related Accession (Same CDS sequence)	NM_168259.3 , NM_001300048.1 , NM_139927.4 , NM_206301.4
Accession Version	NM_139927.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Autophagy-related 18a, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139927.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGCC TGCTCGGGCG CACCGATGTG GATGCGGGCG AAGTGTTCGT CAACTTCAAC 
CAGAACATAA CTTCCCTGGC CGTGGCCACC AGTGGAGGCT ACAGCCTGTA CAGCCTGGGC 
TCCGTGGACT CGACCCTGGA CAAGATCTAC CACACGAAAA GCGACGAGCT GTTCCTCATC 
GAGCGTCTGT TCGAGAGCTC CTTGGTGGCA ATAGTTTCCC AGCGAGCACC ACGCAAGCTC 
AAGGTGTGCC ACTTTAAGAA GCAGAGCGAG ATCTGCAACT ACTCGTATGC AAACACAATA 
CTGGCCGTGA AGCTCAATCG CGAGCGATTG ATTGTGTGCC TGGAGGAGTC ATTGTATATC 
CACAATATCC AGGACATGAA GGTGGTGCAC ACCATACGCG ACACACCGTG CAATCCGCAG 
GGTCTTTGTG CCCTCTCCTC GTCGTCGGAG CACTGCTATC TGGCCTACCC AGGCAGCGTG 
ACTGCTGGCG AGGTACAGAT CTTTGATGCC ATCAACTTGC ACGCCAAGAC CATGATTCCC 
GCCCACGACA CACCTCTGGC GGCATTGGCC TTTAGTCCCT CGGGCACGGA GATAGCCACG 
GCCAGTGAGC GTGGCACGGT GATCCGAGTC TTCAGCTCCC AGGATGGCAG TCGACTCTTT 
GAACTGCGAC GGGGACTAAA GCGCTGTGTG TCCATCGTTT CGCTGTCGTT TAGCACGTGC 
GCCGAATATC TGGTGTCCAG CTCCAACACC GAGACGGTTC ACATCTTCCG GCTGGATCGC 
AGTGCCACGG AAACAGCCGA AGGCCATGGC AGCAAGCAGT CGAGCGACGA CTGGATGGGT 
TACTCGTTTT TTAGATTCTT AAGCAAAACG GTCACCAGCT ACCTGCCCAC GCAGGTGACC 
GACGTGTTTA GCCAAGGCCG TGCCTTCGCC TCGGTCACTC TGCCGGAGGC GGGAGTGCGC 
AGGATGTGCG CTATTGCCAC GATACAAAAG CAGCTAAGAC TTTTGATTGC CTCGCAGGAT 
GGGTACTTGT ACGTGTATTC CATTCCCACC GTCGAGGGCG CAGAGTGCCA GCTAATAAAG 
AGGCACGACC TGCGGCTGGA GGACCACTAT GCCATGGATA TCAAAGGTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648184.1
CDS	227..1360
Translation	MMSLLGRTDVDAGEVFVNFNQNITSLAVATSGGYSLYSLGSVDSTLDKIYHTKSDELFLIERLFESSLVAIVSQRAPRKLKVCHFKKQSEICNYSYANTILAVKLNRERLIVCLEESLYIHNIQDMKVVHTIRDTPCNPQGLCALSSSSEHCYLAYPGSVTAGEVQIFDAINLHAKTMIPAHDTPLAALAFSPSGTEIATASERGTVIRVFSSQDGSRLFELRRGLKRCVSIVSLSFSTCAEYLVSSSNTETVHIFRLDRSATETAEGHGSKQSSDDWMGYSFFRFLSKTVTSYLPTQVTDVFSQGRAFASVTLPEAGVRRMCAIATIQKQLRLLIASQDGYLYVYSIPTVEGAECQLIKRHDLRLEDHYAMDIKGA

Target ORF information:

RefSeq Version	NM_139927.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Autophagy-related 18a (Atg18a), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139927.4

ORF Insert Sequence:

ATGATGAGCC TGCTCGGGCG CACCGATGTG GATGCGGGCG AAGTGTTCGT CAACTTCAAC 
CAGAACATAA CTTCCCTGGC CGTGGCCACC AGTGGAGGCT ACAGCCTGTA CAGCCTGGGC 
TCCGTGGACT CGACCCTGGA CAAGATCTAC CACACGAAAA GCGACGAGCT GTTCCTCATC 
GAGCGTCTGT TCGAGAGCTC CTTGGTGGCA ATAGTTTCCC AGCGAGCACC ACGCAAGCTC 
AAGGTGTGCC ACTTTAAGAA GCAGAGCGAG ATCTGCAACT ACTCGTATGC AAACACAATA 
CTGGCCGTGA AGCTCAATCG CGAGCGATTG ATTGTGTGCC TGGAGGAGTC ATTGTATATC 
CACAATATCC AGGACATGAA GGTGGTGCAC ACCATACGCG ACACACCGTG CAATCCGCAG 
GGTCTTTGTG CCCTCTCCTC GTCGTCGGAG CACTGCTATC TGGCCTACCC AGGCAGCGTG 
ACTGCTGGCG AGGTACAGAT CTTTGATGCC ATCAACTTGC ACGCCAAGAC CATGATTCCC 
GCCCACGACA CACCTCTGGC GGCATTGGCC TTTAGTCCCT CGGGCACGGA GATAGCCACG 
GCCAGTGAGC GTGGCACGGT GATCCGAGTC TTCAGCTCCC AGGATGGCAG TCGACTCTTT 
GAACTGCGAC GGGGACTAAA GCGCTGTGTG TCCATCGTTT CGCTGTCGTT TAGCACGTGC 
GCCGAATATC TGGTGTCCAG CTCCAACACC GAGACGGTTC ACATCTTCCG GCTGGATCGC 
AGTGCCACGG AAACAGCCGA AGGCCATGGC AGCAAGCAGT CGAGCGACGA CTGGATGGGT 
TACTCGTTTT TTAGATTCTT AAGCAAAACG GTCACCAGCT ACCTGCCCAC GCAGGTGACC 
GACGTGTTTA GCCAAGGCCG TGCCTTCGCC TCGGTCACTC TGCCGGAGGC GGGAGTGCGC 
AGGATGTGCG CTATTGCCAC GATACAAAAG CAGCTAAGAC TTTTGATTGC CTCGCAGGAT 
GGGTACTTGT ACGTGTATTC CATTCCCACC GTCGAGGGCG CAGAGTGCCA GCTAATAAAG 
AGGCACGACC TGCGGCTGGA GGACCACTAT GCCATGGATA TCAAAGGTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atg18a ( NM_206301.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996023.1 Drosophila melanogaster Autophagy-related 18a (Atg18a), transcript variant C, mRNA.

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CloneID	OFa17879
Clone ID Related Accession (Same CDS sequence)	NM_168259.3 , NM_001300048.1 , NM_139927.4 , NM_206301.4
Accession Version	NM_206301.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Autophagy-related 18a, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_206301.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGCC TGCTCGGGCG CACCGATGTG GATGCGGGCG AAGTGTTCGT CAACTTCAAC 
CAGAACATAA CTTCCCTGGC CGTGGCCACC AGTGGAGGCT ACAGCCTGTA CAGCCTGGGC 
TCCGTGGACT CGACCCTGGA CAAGATCTAC CACACGAAAA GCGACGAGCT GTTCCTCATC 
GAGCGTCTGT TCGAGAGCTC CTTGGTGGCA ATAGTTTCCC AGCGAGCACC ACGCAAGCTC 
AAGGTGTGCC ACTTTAAGAA GCAGAGCGAG ATCTGCAACT ACTCGTATGC AAACACAATA 
CTGGCCGTGA AGCTCAATCG CGAGCGATTG ATTGTGTGCC TGGAGGAGTC ATTGTATATC 
CACAATATCC AGGACATGAA GGTGGTGCAC ACCATACGCG ACACACCGTG CAATCCGCAG 
GGTCTTTGTG CCCTCTCCTC GTCGTCGGAG CACTGCTATC TGGCCTACCC AGGCAGCGTG 
ACTGCTGGCG AGGTACAGAT CTTTGATGCC ATCAACTTGC ACGCCAAGAC CATGATTCCC 
GCCCACGACA CACCTCTGGC GGCATTGGCC TTTAGTCCCT CGGGCACGGA GATAGCCACG 
GCCAGTGAGC GTGGCACGGT GATCCGAGTC TTCAGCTCCC AGGATGGCAG TCGACTCTTT 
GAACTGCGAC GGGGACTAAA GCGCTGTGTG TCCATCGTTT CGCTGTCGTT TAGCACGTGC 
GCCGAATATC TGGTGTCCAG CTCCAACACC GAGACGGTTC ACATCTTCCG GCTGGATCGC 
AGTGCCACGG AAACAGCCGA AGGCCATGGC AGCAAGCAGT CGAGCGACGA CTGGATGGGT 
TACTCGTTTT TTAGATTCTT AAGCAAAACG GTCACCAGCT ACCTGCCCAC GCAGGTGACC 
GACGTGTTTA GCCAAGGCCG TGCCTTCGCC TCGGTCACTC TGCCGGAGGC GGGAGTGCGC 
AGGATGTGCG CTATTGCCAC GATACAAAAG CAGCTAAGAC TTTTGATTGC CTCGCAGGAT 
GGGTACTTGT ACGTGTATTC CATTCCCACC GTCGAGGGCG CAGAGTGCCA GCTAATAAAG 
AGGCACGACC TGCGGCTGGA GGACCACTAT GCCATGGATA TCAAAGGTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996023.1
CDS	252..1385
Translation	MMSLLGRTDVDAGEVFVNFNQNITSLAVATSGGYSLYSLGSVDSTLDKIYHTKSDELFLIERLFESSLVAIVSQRAPRKLKVCHFKKQSEICNYSYANTILAVKLNRERLIVCLEESLYIHNIQDMKVVHTIRDTPCNPQGLCALSSSSEHCYLAYPGSVTAGEVQIFDAINLHAKTMIPAHDTPLAALAFSPSGTEIATASERGTVIRVFSSQDGSRLFELRRGLKRCVSIVSLSFSTCAEYLVSSSNTETVHIFRLDRSATETAEGHGSKQSSDDWMGYSFFRFLSKTVTSYLPTQVTDVFSQGRAFASVTLPEAGVRRMCAIATIQKQLRLLIASQDGYLYVYSIPTVEGAECQLIKRHDLRLEDHYAMDIKGA

Target ORF information:

RefSeq Version	NM_206301.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Autophagy-related 18a (Atg18a), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206301.4

ORF Insert Sequence:

ATGATGAGCC TGCTCGGGCG CACCGATGTG GATGCGGGCG AAGTGTTCGT CAACTTCAAC 
CAGAACATAA CTTCCCTGGC CGTGGCCACC AGTGGAGGCT ACAGCCTGTA CAGCCTGGGC 
TCCGTGGACT CGACCCTGGA CAAGATCTAC CACACGAAAA GCGACGAGCT GTTCCTCATC 
GAGCGTCTGT TCGAGAGCTC CTTGGTGGCA ATAGTTTCCC AGCGAGCACC ACGCAAGCTC 
AAGGTGTGCC ACTTTAAGAA GCAGAGCGAG ATCTGCAACT ACTCGTATGC AAACACAATA 
CTGGCCGTGA AGCTCAATCG CGAGCGATTG ATTGTGTGCC TGGAGGAGTC ATTGTATATC 
CACAATATCC AGGACATGAA GGTGGTGCAC ACCATACGCG ACACACCGTG CAATCCGCAG 
GGTCTTTGTG CCCTCTCCTC GTCGTCGGAG CACTGCTATC TGGCCTACCC AGGCAGCGTG 
ACTGCTGGCG AGGTACAGAT CTTTGATGCC ATCAACTTGC ACGCCAAGAC CATGATTCCC 
GCCCACGACA CACCTCTGGC GGCATTGGCC TTTAGTCCCT CGGGCACGGA GATAGCCACG 
GCCAGTGAGC GTGGCACGGT GATCCGAGTC TTCAGCTCCC AGGATGGCAG TCGACTCTTT 
GAACTGCGAC GGGGACTAAA GCGCTGTGTG TCCATCGTTT CGCTGTCGTT TAGCACGTGC 
GCCGAATATC TGGTGTCCAG CTCCAACACC GAGACGGTTC ACATCTTCCG GCTGGATCGC 
AGTGCCACGG AAACAGCCGA AGGCCATGGC AGCAAGCAGT CGAGCGACGA CTGGATGGGT 
TACTCGTTTT TTAGATTCTT AAGCAAAACG GTCACCAGCT ACCTGCCCAC GCAGGTGACC 
GACGTGTTTA GCCAAGGCCG TGCCTTCGCC TCGGTCACTC TGCCGGAGGC GGGAGTGCGC 
AGGATGTGCG CTATTGCCAC GATACAAAAG CAGCTAAGAC TTTTGATTGC CTCGCAGGAT 
GGGTACTTGT ACGTGTATTC CATTCCCACC GTCGAGGGCG CAGAGTGCCA GCTAATAAAG 
AGGCACGACC TGCGGCTGGA GGACCACTAT GCCATGGATA TCAAAGGTGC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atg18a ( NM_001274635.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261564.1 Drosophila melanogaster Autophagy-related 18a (Atg18a), transcript variant D, mRNA.

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CloneID	OFa17880
Clone ID Related Accession (Same CDS sequence)	NM_001274635.1
Accession Version	NM_001274635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Autophagy-related 18a, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGCC TGCTCGGGCG CACCGATGTG GATGCGGGCG AAGTGTTCGT CAACTTCAAC 
CAGAACATAA CTTCCCTGGC CGTGGCCACC AGTGGAGGCT ACAGCCTGTA CAGCCTGGGC 
TCCGTGGACT CGACCCTGGA CAAGATCTAC CACACGAAAA GCGACGAGCT GTTCCTCATC 
GAGCGTCTGT TCGAGAGCTC CTTGGTGGCA ATAGTTTCCC AGCGAGCACC ACGCAAGCTC 
AAGGTGTGCC ACTTTAAGAA GCAGAGCGAG ATCTGCAACT ACTCGTATGC AAACACAATA 
CTGGCCGTGA AGCTCAATCG CGAGCGATTG ATTGTGTGCC TGGAGGAGTC ATTGTATATC 
CACAATATCC AGGACATGAA GGTGGTGCAC ACCATACGCG ACACACCGTG CAATCCGCAG 
GGTCTTTGTG CCCTCTCCTC GTCGTCGGAG CACTGCTATC TGGCCTACCC AGGCAGCGTG 
ACTGCTGGCG AGGTACAGAT CTTTGATGCC ATCAACTTGC ACGCCAAGAC CATGATTCCC 
GCCCACGACA CACCTCTGGC GGCATTGGCC TTTAGTCCCT CGGGCACGGA GATAGCCACG 
GCCAGTGAGC GTGGCACGGT GATCCGAGTC TTCAGCTCCC AGGATGGCAG TCGACTCTTT 
GAACTGCGAC GGGGACTAAA GCGCTGTGTG TCCATCGTTT CGCTGTCGTT TAGCACGTGC 
GCCGAATATC TGGTGTCCAG CTCCAACACC GAGACGGTTC ACATCTTCCG GCTGGATCGC 
AGTGCCACGG AAACAGCCGA AGGCCATGGC AGCAAGCAGT CGAGCGACGA CTGGATGGGA 
TTCTTAAGCA AAACGGTCAC CAGCTACCTG CCCACGCAGG TGACCGACGT GTTTAGCCAA 
GGCCGTGCCT TCGCCTCGGT CACTCTGCCG GAGGCGGGAG TGCGCAGGAT GTGCGCTATT 
GCCACGATAC AAAAGCAGCT AAGACTTTTG ATTGCCTCGC AGGATGGGTA CTTGTACGTG 
TATTCCATTC CCACCGTCGA GGGCGCAGAG TGCCAGCTAA TAAAGAGGCA CGACCTGCGG 
CTGGAGGACC ACTATGCCAT GGATATCAAA GGCCTTAACA GTGGCGTGAG CATTGGCGGA 
GCAGCTGGAG TTCCGCCGAG CAGTGCAAGT GCAGCCGGAG CAGGCACTGG TGGAAGTGGC 
GGTGATGGCA AGTCGGCTGT TTCTGTTACC GGTGGTGTCG ACAAACCAAG TAGCTCCTAT 
GCGTCGGCCG TGAAGGGTGA CGATCCCGTG GGACCCTCAT CCGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261564.1
CDS	227..1534
Translation	MMSLLGRTDVDAGEVFVNFNQNITSLAVATSGGYSLYSLGSVDSTLDKIYHTKSDELFLIERLFESSLVAIVSQRAPRKLKVCHFKKQSEICNYSYANTILAVKLNRERLIVCLEESLYIHNIQDMKVVHTIRDTPCNPQGLCALSSSSEHCYLAYPGSVTAGEVQIFDAINLHAKTMIPAHDTPLAALAFSPSGTEIATASERGTVIRVFSSQDGSRLFELRRGLKRCVSIVSLSFSTCAEYLVSSSNTETVHIFRLDRSATETAEGHGSKQSSDDWMGFLSKTVTSYLPTQVTDVFSQGRAFASVTLPEAGVRRMCAIATIQKQLRLLIASQDGYLYVYSIPTVEGAECQLIKRHDLRLEDHYAMDIKGLNSGVSIGGAAGVPPSSASAAGAGTGGSGGDGKSAVSVTGGVDKPSSSYASAVKGDDPVGPSSA

Target ORF information:

RefSeq Version	NM_001274635.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Autophagy-related 18a (Atg18a), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274635.1

ORF Insert Sequence:

ATGATGAGCC TGCTCGGGCG CACCGATGTG GATGCGGGCG AAGTGTTCGT CAACTTCAAC 
CAGAACATAA CTTCCCTGGC CGTGGCCACC AGTGGAGGCT ACAGCCTGTA CAGCCTGGGC 
TCCGTGGACT CGACCCTGGA CAAGATCTAC CACACGAAAA GCGACGAGCT GTTCCTCATC 
GAGCGTCTGT TCGAGAGCTC CTTGGTGGCA ATAGTTTCCC AGCGAGCACC ACGCAAGCTC 
AAGGTGTGCC ACTTTAAGAA GCAGAGCGAG ATCTGCAACT ACTCGTATGC AAACACAATA 
CTGGCCGTGA AGCTCAATCG CGAGCGATTG ATTGTGTGCC TGGAGGAGTC ATTGTATATC 
CACAATATCC AGGACATGAA GGTGGTGCAC ACCATACGCG ACACACCGTG CAATCCGCAG 
GGTCTTTGTG CCCTCTCCTC GTCGTCGGAG CACTGCTATC TGGCCTACCC AGGCAGCGTG 
ACTGCTGGCG AGGTACAGAT CTTTGATGCC ATCAACTTGC ACGCCAAGAC CATGATTCCC 
GCCCACGACA CACCTCTGGC GGCATTGGCC TTTAGTCCCT CGGGCACGGA GATAGCCACG 
GCCAGTGAGC GTGGCACGGT GATCCGAGTC TTCAGCTCCC AGGATGGCAG TCGACTCTTT 
GAACTGCGAC GGGGACTAAA GCGCTGTGTG TCCATCGTTT CGCTGTCGTT TAGCACGTGC 
GCCGAATATC TGGTGTCCAG CTCCAACACC GAGACGGTTC ACATCTTCCG GCTGGATCGC 
AGTGCCACGG AAACAGCCGA AGGCCATGGC AGCAAGCAGT CGAGCGACGA CTGGATGGGA 
TTCTTAAGCA AAACGGTCAC CAGCTACCTG CCCACGCAGG TGACCGACGT GTTTAGCCAA 
GGCCGTGCCT TCGCCTCGGT CACTCTGCCG GAGGCGGGAG TGCGCAGGAT GTGCGCTATT 
GCCACGATAC AAAAGCAGCT AAGACTTTTG ATTGCCTCGC AGGATGGGTA CTTGTACGTG 
TATTCCATTC CCACCGTCGA GGGCGCAGAG TGCCAGCTAA TAAAGAGGCA CGACCTGCGG 
CTGGAGGACC ACTATGCCAT GGATATCAAA GGCCTTAACA GTGGCGTGAG CATTGGCGGA 
GCAGCTGGAG TTCCGCCGAG CAGTGCAAGT GCAGCCGGAG CAGGCACTGG TGGAAGTGGC 
GGTGATGGCA AGTCGGCTGT TTCTGTTACC GGTGGTGTCG ACAAACCAAG TAGCTCCTAT 
GCGTCGGCCG TGAAGGGTGA CGATCCCGTG GGACCCTCAT CCGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atg18a ( NM_001274636.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261565.1 Drosophila melanogaster Autophagy-related 18a (Atg18a), transcript variant E, mRNA.

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CloneID	OFa20695
Clone ID Related Accession (Same CDS sequence)	NM_001274636.1
Accession Version	NM_001274636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Autophagy-related 18a, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGCC TGCTCGGGCG CACCGATGTG GATGCGGGCG AAGTGTTCGT CAACTTCAAC 
CAGAACATAA CTTCCCTGGC CGTGGCCACC AGTGGAGGCT ACAGCCTGTA CAGCCTGGGC 
TCCGTGGACT CGACCCTGGA CAAGATCTAC CACACGAAAA GCGACGAGCT GTTCCTCATC 
GAGCGTCTGT TCGAGAGCTC CTTGGTGGCA ATAGTTTCCC AGCGAGCACC ACGCAAGCTC 
AAGGTGTGCC ACTTTAAGAA GCAGAGCGAG ATCTGCAACT ACTCGTATGC AAACACAATA 
CTGGCCGTGA AGCTCAATCG CGAGCGATTG ATTGTGTGCC TGGAGGAGTC ATTGTATATC 
CACAATATCC AGGACATGAA GGTGGTGCAC ACCATACGCG ACACACCGTG CAATCCGCAG 
GGTCTTTGTG CCCTCTCCTC GTCGTCGGAG CACTGCTATC TGGCCTACCC AGGCAGCGTG 
ACTGCTGGCG AGGTACAGAT CTTTGATGCC ATCAACTTGC ACGCCAAGAC CATGATTCCC 
GCCCACGACA CACCTCTGGC GGCATTGGCC TTTAGTCCCT CGGGCACGGA GATAGCCACG 
GCCAGTGAGC GTGGCACGGT GATCCGAGTC TTCAGCTCCC AGGATGGCAG TCGACTCTTT 
GAACTGCGAC GGGGACTAAA GCGCTGTGTG TCCATCGTTT CGCTGTCGTT TAGCACGTGC 
GCCGAATATC TGGTGTCCAG CTCCAACACC GAGACGGTTC ACATCTTCCG GCTGGATCGC 
AGTGCCACGG AAACAGCCGA AGGCCATGGC AGCAAGCAGT CGAGCGACGA CTGGATGGGT 
TACTCGTTTT TTAGATTCTT AAGCAAAACG GTCACCAGCT ACCTGCCCAC GCAGGTGACC 
GACGTGTTTA GCCAAGGCCG TGCCTTCGCC TCGGTCACTC TGCCGGAGGC GGGAGTGCGC 
AGGATGTGCG CTATTGCCAC GATACAAAAG CAGCTAAGAC TTTTGATTGC CTCGCAGGAT 
GGGTACTTGT ACGTGTATTC CATTCCCACC GTCGAGGGCG CAGAGTGCCA GCTAATAAAG 
AGGCACGACC TGCGGCTGGA GGACCACTAT GCCATGGATA TCAAAGTTGA TTCACCTCAA 
ACTCTAGGCC TTAACAGTGG CGTGAGCATT GGCGGAGCAG CTGGAGTTCC GCCGAGCAGT 
GCAAGTGCAG CCGGAGCAGG CACTGGTGGA AGTGGCGGTG ATGGCAAGTC GGCTGTTTCT 
GTTACCGGTG GTGTCGACAA ACCAAGTAGC TCCTATGCGT CGGCCGTGAA GGGTGACGAT 
CCCGTGGGAC CCTCATCCGC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261565.1
CDS	227..1570
Translation	MMSLLGRTDVDAGEVFVNFNQNITSLAVATSGGYSLYSLGSVDSTLDKIYHTKSDELFLIERLFESSLVAIVSQRAPRKLKVCHFKKQSEICNYSYANTILAVKLNRERLIVCLEESLYIHNIQDMKVVHTIRDTPCNPQGLCALSSSSEHCYLAYPGSVTAGEVQIFDAINLHAKTMIPAHDTPLAALAFSPSGTEIATASERGTVIRVFSSQDGSRLFELRRGLKRCVSIVSLSFSTCAEYLVSSSNTETVHIFRLDRSATETAEGHGSKQSSDDWMGYSFFRFLSKTVTSYLPTQVTDVFSQGRAFASVTLPEAGVRRMCAIATIQKQLRLLIASQDGYLYVYSIPTVEGAECQLIKRHDLRLEDHYAMDIKVDSPQTLGLNSGVSIGGAAGVPPSSASAAGAGTGGSGGDGKSAVSVTGGVDKPSSSYASAVKGDDPVGPSSA

Target ORF information:

RefSeq Version	NM_001274636.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Autophagy-related 18a (Atg18a), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274636.1

ORF Insert Sequence:

ATGATGAGCC TGCTCGGGCG CACCGATGTG GATGCGGGCG AAGTGTTCGT CAACTTCAAC 
CAGAACATAA CTTCCCTGGC CGTGGCCACC AGTGGAGGCT ACAGCCTGTA CAGCCTGGGC 
TCCGTGGACT CGACCCTGGA CAAGATCTAC CACACGAAAA GCGACGAGCT GTTCCTCATC 
GAGCGTCTGT TCGAGAGCTC CTTGGTGGCA ATAGTTTCCC AGCGAGCACC ACGCAAGCTC 
AAGGTGTGCC ACTTTAAGAA GCAGAGCGAG ATCTGCAACT ACTCGTATGC AAACACAATA 
CTGGCCGTGA AGCTCAATCG CGAGCGATTG ATTGTGTGCC TGGAGGAGTC ATTGTATATC 
CACAATATCC AGGACATGAA GGTGGTGCAC ACCATACGCG ACACACCGTG CAATCCGCAG 
GGTCTTTGTG CCCTCTCCTC GTCGTCGGAG CACTGCTATC TGGCCTACCC AGGCAGCGTG 
ACTGCTGGCG AGGTACAGAT CTTTGATGCC ATCAACTTGC ACGCCAAGAC CATGATTCCC 
GCCCACGACA CACCTCTGGC GGCATTGGCC TTTAGTCCCT CGGGCACGGA GATAGCCACG 
GCCAGTGAGC GTGGCACGGT GATCCGAGTC TTCAGCTCCC AGGATGGCAG TCGACTCTTT 
GAACTGCGAC GGGGACTAAA GCGCTGTGTG TCCATCGTTT CGCTGTCGTT TAGCACGTGC 
GCCGAATATC TGGTGTCCAG CTCCAACACC GAGACGGTTC ACATCTTCCG GCTGGATCGC 
AGTGCCACGG AAACAGCCGA AGGCCATGGC AGCAAGCAGT CGAGCGACGA CTGGATGGGT 
TACTCGTTTT TTAGATTCTT AAGCAAAACG GTCACCAGCT ACCTGCCCAC GCAGGTGACC 
GACGTGTTTA GCCAAGGCCG TGCCTTCGCC TCGGTCACTC TGCCGGAGGC GGGAGTGCGC 
AGGATGTGCG CTATTGCCAC GATACAAAAG CAGCTAAGAC TTTTGATTGC CTCGCAGGAT 
GGGTACTTGT ACGTGTATTC CATTCCCACC GTCGAGGGCG CAGAGTGCCA GCTAATAAAG 
AGGCACGACC TGCGGCTGGA GGACCACTAT GCCATGGATA TCAAAGTTGA TTCACCTCAA 
ACTCTAGGCC TTAACAGTGG CGTGAGCATT GGCGGAGCAG CTGGAGTTCC GCCGAGCAGT 
GCAAGTGCAG CCGGAGCAGG CACTGGTGGA AGTGGCGGTG ATGGCAAGTC GGCTGTTTCT 
GTTACCGGTG GTGTCGACAA ACCAAGTAGC TCCTATGCGT CGGCCGTGAA GGGTGACGAT 
CCCGTGGGAC CCTCATCCGC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atg18a ( NM_001274637.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261566.1 Drosophila melanogaster Autophagy-related 18a (Atg18a), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa20696
Clone ID Related Accession (Same CDS sequence)	NM_001274637.1
Accession Version	NM_001274637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Autophagy-related 18a, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGCC TGCTCGGGCG CACCGATGTG GATGCGGGCG AAGTGTTCGT CAACTTCAAC 
CAGAACATAA CTTCCCTGGC CGTGGCCACC AGTGGAGGCT ACAGCCTGTA CAGCCTGGGC 
TCCGTGGACT CGACCCTGGA CAAGATCTAC CACACGAAAA GCGACGAGCT GTTCCTCATC 
GAGCGTCTGT TCGAGAGCTC CTTGGTGGCA ATAGTTTCCC AGCGAGCACC ACGCAAGCTC 
AAGGTGTGCC ACTTTAAGAA GCAGAGCGAG ATCTGCAACT ACTCGTATGC AAACACAATA 
CTGGCCGTGA AGCTCAATCG CGAGCGATTG ATTGTGTGCC TGGAGGAGTC ATTGTATATC 
CACAATATCC AGGACATGAA GGTGGTGCAC ACCATACGCG ACACACCGTG CAATCCGCAG 
GGTCTTTGTG CCCTCTCCTC GTCGTCGGAG CACTGCTATC TGGCCTACCC AGGCAGCGTG 
ACTGCTGGCG AGGTACAGAT CTTTGATGCC ATCAACTTGC ACGCCAAGAC CATGATTCCC 
GCCCACGACA CACCTCTGGC GGCATTGGCC TTTAGTCCCT CGGGCACGGA GATAGCCACG 
GCCAGTGAGC GTGGCACGGT GATCCGAGTC TTCAGCTCCC AGGATGGCAG TCGACTCTTT 
GAACTGCGAC GGGGACTAAA GCGCTGTGTG TCCATCGTTT CGCTGTCGTT TAGCACGTGC 
GCCGAATATC TGGTGTCCAG CTCCAACACC GAGACGGTTC ACATCTTCCG GCTGGATCGC 
AGTGCCACGG AAACAGCCGA AGGCCATGGC AGCAAGCAGT CGAGCGACGA CTGGATGGGA 
TTCTTAAGCA AAACGGTCAC CAGCTACCTG CCCACGCAGG TGACCGACGT GTTTAGCCAA 
GGCCGTGCCT TCGCCTCGGT CACTCTGCCG GAGGCGGGAG TGCGCAGGAT GTGCGCTATT 
GCCACGATAC AAAAGCAGCT AAGACTTTTG ATTGCCTCGC AGGATGGGTA CTTGTACGTG 
TATTCCATTC CCACCGTCGA GGGCGCAGAG TGCCAGCTAA TAAAGAGGCA CGACCTGCGG 
CTGGAGGACC ACTATGCCAT GGATATCAAA GGTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261566.1
CDS	436..1554
Translation	MMSLLGRTDVDAGEVFVNFNQNITSLAVATSGGYSLYSLGSVDSTLDKIYHTKSDELFLIERLFESSLVAIVSQRAPRKLKVCHFKKQSEICNYSYANTILAVKLNRERLIVCLEESLYIHNIQDMKVVHTIRDTPCNPQGLCALSSSSEHCYLAYPGSVTAGEVQIFDAINLHAKTMIPAHDTPLAALAFSPSGTEIATASERGTVIRVFSSQDGSRLFELRRGLKRCVSIVSLSFSTCAEYLVSSSNTETVHIFRLDRSATETAEGHGSKQSSDDWMGFLSKTVTSYLPTQVTDVFSQGRAFASVTLPEAGVRRMCAIATIQKQLRLLIASQDGYLYVYSIPTVEGAECQLIKRHDLRLEDHYAMDIKGA

Target ORF information:

RefSeq Version	NM_001274637.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Autophagy-related 18a (Atg18a), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274637.1

ORF Insert Sequence:

ATGATGAGCC TGCTCGGGCG CACCGATGTG GATGCGGGCG AAGTGTTCGT CAACTTCAAC 
CAGAACATAA CTTCCCTGGC CGTGGCCACC AGTGGAGGCT ACAGCCTGTA CAGCCTGGGC 
TCCGTGGACT CGACCCTGGA CAAGATCTAC CACACGAAAA GCGACGAGCT GTTCCTCATC 
GAGCGTCTGT TCGAGAGCTC CTTGGTGGCA ATAGTTTCCC AGCGAGCACC ACGCAAGCTC 
AAGGTGTGCC ACTTTAAGAA GCAGAGCGAG ATCTGCAACT ACTCGTATGC AAACACAATA 
CTGGCCGTGA AGCTCAATCG CGAGCGATTG ATTGTGTGCC TGGAGGAGTC ATTGTATATC 
CACAATATCC AGGACATGAA GGTGGTGCAC ACCATACGCG ACACACCGTG CAATCCGCAG 
GGTCTTTGTG CCCTCTCCTC GTCGTCGGAG CACTGCTATC TGGCCTACCC AGGCAGCGTG 
ACTGCTGGCG AGGTACAGAT CTTTGATGCC ATCAACTTGC ACGCCAAGAC CATGATTCCC 
GCCCACGACA CACCTCTGGC GGCATTGGCC TTTAGTCCCT CGGGCACGGA GATAGCCACG 
GCCAGTGAGC GTGGCACGGT GATCCGAGTC TTCAGCTCCC AGGATGGCAG TCGACTCTTT 
GAACTGCGAC GGGGACTAAA GCGCTGTGTG TCCATCGTTT CGCTGTCGTT TAGCACGTGC 
GCCGAATATC TGGTGTCCAG CTCCAACACC GAGACGGTTC ACATCTTCCG GCTGGATCGC 
AGTGCCACGG AAACAGCCGA AGGCCATGGC AGCAAGCAGT CGAGCGACGA CTGGATGGGA 
TTCTTAAGCA AAACGGTCAC CAGCTACCTG CCCACGCAGG TGACCGACGT GTTTAGCCAA 
GGCCGTGCCT TCGCCTCGGT CACTCTGCCG GAGGCGGGAG TGCGCAGGAT GTGCGCTATT 
GCCACGATAC AAAAGCAGCT AAGACTTTTG ATTGCCTCGC AGGATGGGTA CTTGTACGTG 
TATTCCATTC CCACCGTCGA GGGCGCAGAG TGCCAGCTAA TAAAGAGGCA CGACCTGCGG 
CTGGAGGACC ACTATGCCAT GGATATCAAA GGTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Atg18a cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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