nmo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	nmo
Entrez Gene ID	38890
Full Name	nemo
Synonyms	CG7892,Dmel\CG7892,NEMO,NEMO/NLK,NLK,Nemo,Nlk,Nmo,ORE-6,adk,adk1,anon-EST:Gibbs3,anon-EST:Liang-2.43,anon-WO0118547.332,anon-WO0140519.256,anon-WO0172774.138,clone 2.43
General protein information	Preferred Names nemo Names CG7892 gene product from transcript CG7892-RH CG7892-PA CG7892-PB CG7892-PC CG7892-PE CG7892-PF CG7892-PG CG7892-PH NEMO Nemo-like kinase adirondack dNEMO nmo-PA nmo-PB nmo-PC nmo-PE nmo-PF nmo-PG nmo-PH
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66A22-66B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_168252.3	NP_729320.1	nemo, isoform B
NM_079243.4	NP_523967.2	nemo, isoform C
NM_001274626.1	NP_001261555.1	nemo, isoform H
NM_001370048.1	NP_001356976.1	nemo, isoform I
NM_168251.3	NP_729319.1	nemo, isoform A
NM_168249.3	NP_729317.1	nemo, isoform F
NM_168248.3	NP_729316.1	nemo, isoform E
NM_168250.3	NP_729318.1	nemo, isoform G

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nlk	NP_032728.3
Homo sapiens (human)	NLK	NP_057315.3
Gallus gallus (chicken)	NLK	XP_415915.3
Drosophila melanogaster (fruit fly)	nmo	NP_729318.1
Caenorhabditis elegans (roundworm)	lit-1	NP_001022806.1
Rattus norvegicus (Norway rat)	Nlk	NP_001178853.1
Danio rerio (zebrafish)	nlk2	NP_001106957.1
Pan troglodytes (chimpanzee)	NLK	XP_001147662.2
Canis lupus familiaris (dog)	NLK	XP_868108.2
Bos taurus (cattle)	NLK	NP_001180182.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005898	XP_315928.3
Xenopus tropicalis (tropical clawed frog)	nlk	NP_001116885.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

nmo promotes Notch-mediated lateral inhibition downstream of proneural factors during neurogenesis.
Title: Nemo promotes Notch-mediated lateral inhibition downstream of proneural factors.

One of the roles of Nmo during ommatial rotation is to repress miple expression, which may in turn affect the dynamics in E-cadherin-beta-catenin complexes.
Title: Nemo regulates cell dynamics and represses the expression of miple, a midkine/pleiotrophin cytokine, during ommatidial rotation.

These data suggest that Nemo serves as a conserved molecular link between strabismus and prickle proteins and E-cadherin-beta-catenin complexes, promoting cell motility.
Title: Nemo kinase phosphorylates ?-catenin to promote ommatidial rotation and connects core PCP factors to E-cadherin-?-catenin.

NEMO kinase contributes to core period determination by slowing the pace of the Drosophila circadian oscillator.
Title: NEMO kinase contributes to core period determination by slowing the pace of the Drosophila circadian oscillator.

Findings identify NEMO as a clock kinase and demonstrate that long-range interactions between functionally distinct phospho-clusters collaborate to set circadian clock speed.
Title: NEMO/NLK phosphorylates PERIOD to initiate a time-delay phosphorylation circuit that sets circadian clock speed.

The following nmo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nmo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17855	NM_079243.4 Latest version!	Drosophila melanogaster nemo (nmo), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa17855	NM_168252.3 Latest version!	Drosophila melanogaster nemo (nmo), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa17855	NM_168251.3 Latest version!	Drosophila melanogaster nemo (nmo), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa17856	NM_168249.3 Latest version!	Drosophila melanogaster nemo (nmo), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa17856	NM_168250.3 Latest version!	Drosophila melanogaster nemo (nmo), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa17856	NM_168248.3 Latest version!	Drosophila melanogaster nemo (nmo), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa17856	NM_001274626.1 Latest version!	Drosophila melanogaster nemo (nmo), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa87761	NM_001370048.1 Latest version!	Drosophila melanogaster nemo (nmo), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nmo ( NM_168252.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729320.1 Drosophila melanogaster nemo (nmo), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17855
Clone ID Related Accession (Same CDS sequence)	NM_168252.3 , NM_079243.4 , NM_168251.3
Accession Version	NM_168252.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nemo, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168252.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGTTT CGATGTCACT GGTACAGGGT GGTGCTGCCG GTGGTGCGCC ACAGGGCGCC 
AGCGCAATCT TGGCCGCAGC GGCTCCCTAC TATCAACCGC CGGCTGTTCC CCAGGATGTT 
CAGCCGGATC GTCCTATCGG CTATGGAGCA TTCGGAGTTG TCTGGGCCGT CACAGATCCT 
CGCGATGGAC GACGAGTGGC ATTGAAGAAG CTGCCAAATG TGTTCCAATC GTTGGTCTCA 
TCGAAGCGCG TTTTTCGCGA GCTGAAAATG CTGTGCTTCT TCAAGCATGA AAATGTTCTT 
TCAGCTCTGG ACATTCTACA GCCACCGCAT TTGGATTTCT TTCAAGAAAT CTATGTGATA 
ACGGAGCTGC TGCAATCGGA TCTGCACAAG ATAATCGTGT CGCCACAGCA CCTGTCCGCC 
GACCACATCA AGGTGTTCCT GTACCAGATC CTGCGCGGCC TCAAATATCT GCACTCGGCC 
CGCATCCTGC ATCGCGACAT CAAGCCGGGC AATCTGCTGG TCAACTCGAA TTGCGTGCTC 
AAGATCTGTG ACTTTGGATT GGCCCGCGTC GAGGAGCCGG ATCAGGCCAA GCACATGACC 
CAAGAGGTGG TCACCCAGTA CTATCGCGCA CCGGAGATTC TGATGGGCGC TCGCCACTAC 
TCGTCGGCGG TGGATGTATG GTCGGTGGGC TGCATTTTTG GCGAGCTGCT GGGCCGTCGC 
ATCCTCTTCC AGGCACAGAA TCCTGTGCAG CAGCTGGAGC TGATTACCGA GCTGCTTGGC 
ACGCCCACCA TGGAGGATAT GCGTCACGCC TGCGAGGGCG CCCGCACCCA CATGTTGCGC 
CGGGCGCCCA AGCCGCCATC CTTCTCGGTG CTCTACACAC TCAGCTCGCA TGCCACGCAC 
GAGGCGGTGC ACTTGCTCTG CCAGATGCTC GTCTTCGATC CGGATAAGCG CATCTCCGTG 
ACAGATGCCC TGGCGCATCC GTATCTGGAT GAGGGGCGTC TGCGCTATCA CTCGTGCATG 
TGCAAATGCT GCTTCACTAC CTCAGCCGGA ATGCGTCAGT ATACGGCTGA TTTTGAGCCC 
TCCGCCGGCC AGCCCTTCGA TGATCTGTGG GAACGTAAGC TGACATCCGT GCAGCAGGTC 
AAAGAGGAGA TGCACAAGTT CATTGCCGAG CAGCTGCAAA CGGGTCGTGT GCCGCTGTGC 
ATCAATCCAC AGAGTGCAGC CTTCAAGAGT TTCGCCAGCC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729320.1
CDS	1055..2299
Translation	MSVSMSLVQGGAAGGAPQGASAILAAAAPYYQPPAVPQDVQPDRPIGYGAFGVVWAVTDPRDGRRVALKKLPNVFQSLVSSKRVFRELKMLCFFKHENVLSALDILQPPHLDFFQEIYVITELLQSDLHKIIVSPQHLSADHIKVFLYQILRGLKYLHSARILHRDIKPGNLLVNSNCVLKICDFGLARVEEPDQAKHMTQEVVTQYYRAPEILMGARHYSSAVDVWSVGCIFGELLGRRILFQAQNPVQQLELITELLGTPTMEDMRHACEGARTHMLRRAPKPPSFSVLYTLSSHATHEAVHLLCQMLVFDPDKRISVTDALAHPYLDEGRLRYHSCMCKCCFTTSAGMRQYTADFEPSAGQPFDDLWERKLTSVQQVKEEMHKFIAEQLQTGRVPLCINPQSAAFKSFASQ

Target ORF information:

RefSeq Version	NM_168252.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nemo (nmo), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168252.3

ORF Insert Sequence:

ATGAGCGTTT CGATGTCACT GGTACAGGGT GGTGCTGCCG GTGGTGCGCC ACAGGGCGCC 
AGCGCAATCT TGGCCGCAGC GGCTCCCTAC TATCAACCGC CGGCTGTTCC CCAGGATGTT 
CAGCCGGATC GTCCTATCGG CTATGGAGCA TTCGGAGTTG TCTGGGCCGT CACAGATCCT 
CGCGATGGAC GACGAGTGGC ATTGAAGAAG CTGCCAAATG TGTTCCAATC GTTGGTCTCA 
TCGAAGCGCG TTTTTCGCGA GCTGAAAATG CTGTGCTTCT TCAAGCATGA AAATGTTCTT 
TCAGCTCTGG ACATTCTACA GCCACCGCAT TTGGATTTCT TTCAAGAAAT CTATGTGATA 
ACGGAGCTGC TGCAATCGGA TCTGCACAAG ATAATCGTGT CGCCACAGCA CCTGTCCGCC 
GACCACATCA AGGTGTTCCT GTACCAGATC CTGCGCGGCC TCAAATATCT GCACTCGGCC 
CGCATCCTGC ATCGCGACAT CAAGCCGGGC AATCTGCTGG TCAACTCGAA TTGCGTGCTC 
AAGATCTGTG ACTTTGGATT GGCCCGCGTC GAGGAGCCGG ATCAGGCCAA GCACATGACC 
CAAGAGGTGG TCACCCAGTA CTATCGCGCA CCGGAGATTC TGATGGGCGC TCGCCACTAC 
TCGTCGGCGG TGGATGTATG GTCGGTGGGC TGCATTTTTG GCGAGCTGCT GGGCCGTCGC 
ATCCTCTTCC AGGCACAGAA TCCTGTGCAG CAGCTGGAGC TGATTACCGA GCTGCTTGGC 
ACGCCCACCA TGGAGGATAT GCGTCACGCC TGCGAGGGCG CCCGCACCCA CATGTTGCGC 
CGGGCGCCCA AGCCGCCATC CTTCTCGGTG CTCTACACAC TCAGCTCGCA TGCCACGCAC 
GAGGCGGTGC ACTTGCTCTG CCAGATGCTC GTCTTCGATC CGGATAAGCG CATCTCCGTG 
ACAGATGCCC TGGCGCATCC GTATCTGGAT GAGGGGCGTC TGCGCTATCA CTCGTGCATG 
TGCAAATGCT GCTTCACTAC CTCAGCCGGA ATGCGTCAGT ATACGGCTGA TTTTGAGCCC 
TCCGCCGGCC AGCCCTTCGA TGATCTGTGG GAACGTAAGC TGACATCCGT GCAGCAGGTC 
AAAGAGGAGA TGCACAAGTT CATTGCCGAG CAGCTGCAAA CGGGTCGTGT GCCGCTGTGC 
ATCAATCCAC AGAGTGCAGC CTTCAAGAGT TTCGCCAGCC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nmo ( NM_079243.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523967.2 Drosophila melanogaster nemo (nmo), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa17855
Clone ID Related Accession (Same CDS sequence)	NM_168252.3 , NM_079243.4 , NM_168251.3
Accession Version	NM_079243.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nemo, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079243.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGTTT CGATGTCACT GGTACAGGGT GGTGCTGCCG GTGGTGCGCC ACAGGGCGCC 
AGCGCAATCT TGGCCGCAGC GGCTCCCTAC TATCAACCGC CGGCTGTTCC CCAGGATGTT 
CAGCCGGATC GTCCTATCGG CTATGGAGCA TTCGGAGTTG TCTGGGCCGT CACAGATCCT 
CGCGATGGAC GACGAGTGGC ATTGAAGAAG CTGCCAAATG TGTTCCAATC GTTGGTCTCA 
TCGAAGCGCG TTTTTCGCGA GCTGAAAATG CTGTGCTTCT TCAAGCATGA AAATGTTCTT 
TCAGCTCTGG ACATTCTACA GCCACCGCAT TTGGATTTCT TTCAAGAAAT CTATGTGATA 
ACGGAGCTGC TGCAATCGGA TCTGCACAAG ATAATCGTGT CGCCACAGCA CCTGTCCGCC 
GACCACATCA AGGTGTTCCT GTACCAGATC CTGCGCGGCC TCAAATATCT GCACTCGGCC 
CGCATCCTGC ATCGCGACAT CAAGCCGGGC AATCTGCTGG TCAACTCGAA TTGCGTGCTC 
AAGATCTGTG ACTTTGGATT GGCCCGCGTC GAGGAGCCGG ATCAGGCCAA GCACATGACC 
CAAGAGGTGG TCACCCAGTA CTATCGCGCA CCGGAGATTC TGATGGGCGC TCGCCACTAC 
TCGTCGGCGG TGGATGTATG GTCGGTGGGC TGCATTTTTG GCGAGCTGCT GGGCCGTCGC 
ATCCTCTTCC AGGCACAGAA TCCTGTGCAG CAGCTGGAGC TGATTACCGA GCTGCTTGGC 
ACGCCCACCA TGGAGGATAT GCGTCACGCC TGCGAGGGCG CCCGCACCCA CATGTTGCGC 
CGGGCGCCCA AGCCGCCATC CTTCTCGGTG CTCTACACAC TCAGCTCGCA TGCCACGCAC 
GAGGCGGTGC ACTTGCTCTG CCAGATGCTC GTCTTCGATC CGGATAAGCG CATCTCCGTG 
ACAGATGCCC TGGCGCATCC GTATCTGGAT GAGGGGCGTC TGCGCTATCA CTCGTGCATG 
TGCAAATGCT GCTTCACTAC CTCAGCCGGA ATGCGTCAGT ATACGGCTGA TTTTGAGCCC 
TCCGCCGGCC AGCCCTTCGA TGATCTGTGG GAACGTAAGC TGACATCCGT GCAGCAGGTC 
AAAGAGGAGA TGCACAAGTT CATTGCCGAG CAGCTGCAAA CGGGTCGTGT GCCGCTGTGC 
ATCAATCCAC AGAGTGCAGC CTTCAAGAGT TTCGCCAGCC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523967.2
CDS	548..1792
Translation	MSVSMSLVQGGAAGGAPQGASAILAAAAPYYQPPAVPQDVQPDRPIGYGAFGVVWAVTDPRDGRRVALKKLPNVFQSLVSSKRVFRELKMLCFFKHENVLSALDILQPPHLDFFQEIYVITELLQSDLHKIIVSPQHLSADHIKVFLYQILRGLKYLHSARILHRDIKPGNLLVNSNCVLKICDFGLARVEEPDQAKHMTQEVVTQYYRAPEILMGARHYSSAVDVWSVGCIFGELLGRRILFQAQNPVQQLELITELLGTPTMEDMRHACEGARTHMLRRAPKPPSFSVLYTLSSHATHEAVHLLCQMLVFDPDKRISVTDALAHPYLDEGRLRYHSCMCKCCFTTSAGMRQYTADFEPSAGQPFDDLWERKLTSVQQVKEEMHKFIAEQLQTGRVPLCINPQSAAFKSFASQ

Target ORF information:

RefSeq Version	NM_079243.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nemo (nmo), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079243.4

ORF Insert Sequence:

ATGAGCGTTT CGATGTCACT GGTACAGGGT GGTGCTGCCG GTGGTGCGCC ACAGGGCGCC 
AGCGCAATCT TGGCCGCAGC GGCTCCCTAC TATCAACCGC CGGCTGTTCC CCAGGATGTT 
CAGCCGGATC GTCCTATCGG CTATGGAGCA TTCGGAGTTG TCTGGGCCGT CACAGATCCT 
CGCGATGGAC GACGAGTGGC ATTGAAGAAG CTGCCAAATG TGTTCCAATC GTTGGTCTCA 
TCGAAGCGCG TTTTTCGCGA GCTGAAAATG CTGTGCTTCT TCAAGCATGA AAATGTTCTT 
TCAGCTCTGG ACATTCTACA GCCACCGCAT TTGGATTTCT TTCAAGAAAT CTATGTGATA 
ACGGAGCTGC TGCAATCGGA TCTGCACAAG ATAATCGTGT CGCCACAGCA CCTGTCCGCC 
GACCACATCA AGGTGTTCCT GTACCAGATC CTGCGCGGCC TCAAATATCT GCACTCGGCC 
CGCATCCTGC ATCGCGACAT CAAGCCGGGC AATCTGCTGG TCAACTCGAA TTGCGTGCTC 
AAGATCTGTG ACTTTGGATT GGCCCGCGTC GAGGAGCCGG ATCAGGCCAA GCACATGACC 
CAAGAGGTGG TCACCCAGTA CTATCGCGCA CCGGAGATTC TGATGGGCGC TCGCCACTAC 
TCGTCGGCGG TGGATGTATG GTCGGTGGGC TGCATTTTTG GCGAGCTGCT GGGCCGTCGC 
ATCCTCTTCC AGGCACAGAA TCCTGTGCAG CAGCTGGAGC TGATTACCGA GCTGCTTGGC 
ACGCCCACCA TGGAGGATAT GCGTCACGCC TGCGAGGGCG CCCGCACCCA CATGTTGCGC 
CGGGCGCCCA AGCCGCCATC CTTCTCGGTG CTCTACACAC TCAGCTCGCA TGCCACGCAC 
GAGGCGGTGC ACTTGCTCTG CCAGATGCTC GTCTTCGATC CGGATAAGCG CATCTCCGTG 
ACAGATGCCC TGGCGCATCC GTATCTGGAT GAGGGGCGTC TGCGCTATCA CTCGTGCATG 
TGCAAATGCT GCTTCACTAC CTCAGCCGGA ATGCGTCAGT ATACGGCTGA TTTTGAGCCC 
TCCGCCGGCC AGCCCTTCGA TGATCTGTGG GAACGTAAGC TGACATCCGT GCAGCAGGTC 
AAAGAGGAGA TGCACAAGTT CATTGCCGAG CAGCTGCAAA CGGGTCGTGT GCCGCTGTGC 
ATCAATCCAC AGAGTGCAGC CTTCAAGAGT TTCGCCAGCC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nmo ( NM_168251.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729319.1 Drosophila melanogaster nemo (nmo), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17855
Clone ID Related Accession (Same CDS sequence)	NM_168252.3 , NM_079243.4 , NM_168251.3
Accession Version	NM_168251.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nemo, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168251.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGTTT CGATGTCACT GGTACAGGGT GGTGCTGCCG GTGGTGCGCC ACAGGGCGCC 
AGCGCAATCT TGGCCGCAGC GGCTCCCTAC TATCAACCGC CGGCTGTTCC CCAGGATGTT 
CAGCCGGATC GTCCTATCGG CTATGGAGCA TTCGGAGTTG TCTGGGCCGT CACAGATCCT 
CGCGATGGAC GACGAGTGGC ATTGAAGAAG CTGCCAAATG TGTTCCAATC GTTGGTCTCA 
TCGAAGCGCG TTTTTCGCGA GCTGAAAATG CTGTGCTTCT TCAAGCATGA AAATGTTCTT 
TCAGCTCTGG ACATTCTACA GCCACCGCAT TTGGATTTCT TTCAAGAAAT CTATGTGATA 
ACGGAGCTGC TGCAATCGGA TCTGCACAAG ATAATCGTGT CGCCACAGCA CCTGTCCGCC 
GACCACATCA AGGTGTTCCT GTACCAGATC CTGCGCGGCC TCAAATATCT GCACTCGGCC 
CGCATCCTGC ATCGCGACAT CAAGCCGGGC AATCTGCTGG TCAACTCGAA TTGCGTGCTC 
AAGATCTGTG ACTTTGGATT GGCCCGCGTC GAGGAGCCGG ATCAGGCCAA GCACATGACC 
CAAGAGGTGG TCACCCAGTA CTATCGCGCA CCGGAGATTC TGATGGGCGC TCGCCACTAC 
TCGTCGGCGG TGGATGTATG GTCGGTGGGC TGCATTTTTG GCGAGCTGCT GGGCCGTCGC 
ATCCTCTTCC AGGCACAGAA TCCTGTGCAG CAGCTGGAGC TGATTACCGA GCTGCTTGGC 
ACGCCCACCA TGGAGGATAT GCGTCACGCC TGCGAGGGCG CCCGCACCCA CATGTTGCGC 
CGGGCGCCCA AGCCGCCATC CTTCTCGGTG CTCTACACAC TCAGCTCGCA TGCCACGCAC 
GAGGCGGTGC ACTTGCTCTG CCAGATGCTC GTCTTCGATC CGGATAAGCG CATCTCCGTG 
ACAGATGCCC TGGCGCATCC GTATCTGGAT GAGGGGCGTC TGCGCTATCA CTCGTGCATG 
TGCAAATGCT GCTTCACTAC CTCAGCCGGA ATGCGTCAGT ATACGGCTGA TTTTGAGCCC 
TCCGCCGGCC AGCCCTTCGA TGATCTGTGG GAACGTAAGC TGACATCCGT GCAGCAGGTC 
AAAGAGGAGA TGCACAAGTT CATTGCCGAG CAGCTGCAAA CGGGTCGTGT GCCGCTGTGC 
ATCAATCCAC AGAGTGCAGC CTTCAAGAGT TTCGCCAGCC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729319.1
CDS	719..1963
Translation	MSVSMSLVQGGAAGGAPQGASAILAAAAPYYQPPAVPQDVQPDRPIGYGAFGVVWAVTDPRDGRRVALKKLPNVFQSLVSSKRVFRELKMLCFFKHENVLSALDILQPPHLDFFQEIYVITELLQSDLHKIIVSPQHLSADHIKVFLYQILRGLKYLHSARILHRDIKPGNLLVNSNCVLKICDFGLARVEEPDQAKHMTQEVVTQYYRAPEILMGARHYSSAVDVWSVGCIFGELLGRRILFQAQNPVQQLELITELLGTPTMEDMRHACEGARTHMLRRAPKPPSFSVLYTLSSHATHEAVHLLCQMLVFDPDKRISVTDALAHPYLDEGRLRYHSCMCKCCFTTSAGMRQYTADFEPSAGQPFDDLWERKLTSVQQVKEEMHKFIAEQLQTGRVPLCINPQSAAFKSFASQ

Target ORF information:

RefSeq Version	NM_168251.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nemo (nmo), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168251.3

ORF Insert Sequence:

ATGAGCGTTT CGATGTCACT GGTACAGGGT GGTGCTGCCG GTGGTGCGCC ACAGGGCGCC 
AGCGCAATCT TGGCCGCAGC GGCTCCCTAC TATCAACCGC CGGCTGTTCC CCAGGATGTT 
CAGCCGGATC GTCCTATCGG CTATGGAGCA TTCGGAGTTG TCTGGGCCGT CACAGATCCT 
CGCGATGGAC GACGAGTGGC ATTGAAGAAG CTGCCAAATG TGTTCCAATC GTTGGTCTCA 
TCGAAGCGCG TTTTTCGCGA GCTGAAAATG CTGTGCTTCT TCAAGCATGA AAATGTTCTT 
TCAGCTCTGG ACATTCTACA GCCACCGCAT TTGGATTTCT TTCAAGAAAT CTATGTGATA 
ACGGAGCTGC TGCAATCGGA TCTGCACAAG ATAATCGTGT CGCCACAGCA CCTGTCCGCC 
GACCACATCA AGGTGTTCCT GTACCAGATC CTGCGCGGCC TCAAATATCT GCACTCGGCC 
CGCATCCTGC ATCGCGACAT CAAGCCGGGC AATCTGCTGG TCAACTCGAA TTGCGTGCTC 
AAGATCTGTG ACTTTGGATT GGCCCGCGTC GAGGAGCCGG ATCAGGCCAA GCACATGACC 
CAAGAGGTGG TCACCCAGTA CTATCGCGCA CCGGAGATTC TGATGGGCGC TCGCCACTAC 
TCGTCGGCGG TGGATGTATG GTCGGTGGGC TGCATTTTTG GCGAGCTGCT GGGCCGTCGC 
ATCCTCTTCC AGGCACAGAA TCCTGTGCAG CAGCTGGAGC TGATTACCGA GCTGCTTGGC 
ACGCCCACCA TGGAGGATAT GCGTCACGCC TGCGAGGGCG CCCGCACCCA CATGTTGCGC 
CGGGCGCCCA AGCCGCCATC CTTCTCGGTG CTCTACACAC TCAGCTCGCA TGCCACGCAC 
GAGGCGGTGC ACTTGCTCTG CCAGATGCTC GTCTTCGATC CGGATAAGCG CATCTCCGTG 
ACAGATGCCC TGGCGCATCC GTATCTGGAT GAGGGGCGTC TGCGCTATCA CTCGTGCATG 
TGCAAATGCT GCTTCACTAC CTCAGCCGGA ATGCGTCAGT ATACGGCTGA TTTTGAGCCC 
TCCGCCGGCC AGCCCTTCGA TGATCTGTGG GAACGTAAGC TGACATCCGT GCAGCAGGTC 
AAAGAGGAGA TGCACAAGTT CATTGCCGAG CAGCTGCAAA CGGGTCGTGT GCCGCTGTGC 
ATCAATCCAC AGAGTGCAGC CTTCAAGAGT TTCGCCAGCC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nmo ( NM_001274626.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261555.1 Drosophila melanogaster nemo (nmo), transcript variant H, mRNA.

Price & Availability↑

CloneID	OFa17856
Clone ID Related Accession (Same CDS sequence)	NM_001274626.1 , NM_168249.3 , NM_168248.3 , NM_168250.3
Accession Version	NM_001274626.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nemo, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGTTT CGATGTCACT GGTACAGGGT GGTGCTGCCG GTGGTGCGCC ACAGGGCGCC 
AGCGCAATCT TGGCCGCAGC GGCTCCCTAC TATCAACCGC CGGCTGTTCC CCAGGATGTT 
CAGCCGGATC GTCCTATCGG CTATGGAGCA TTCGGAGTTG TCTGGGCCGT CACAGATCCT 
CGCGATGGAC GACGAGTGGC ATTGAAGAAG CTGCCAAATG TGTTCCAATC GTTGGTCTCA 
TCGAAGCGCG TTTTTCGCGA GCTGAAAATG CTGTGCTTCT TCAAGCATGA AAATGTTCTT 
TCAGCTCTGG ACATTCTACA GCCACCGCAT TTGGATTTCT TTCAAGAAAT CTATGTGATA 
ACGGAGCTGC TGCAATCGGA TCTGCACAAG ATAATCGTGT CGCCACAGCA CCTGTCCGCC 
GACCACATCA AGGTGTTCCT GTACCAGATC CTGCGCGGCC TCAAATATCT GCACTCGGCC 
CGCATCCTGC ATCGCGACAT CAAGCCGGGC AATCTGCTGG TCAACTCGAA TTGCGTGCTC 
AAGATCTGTG ACTTTGGATT GGCCCGCGTC GAGGAGCCGG ATCAGGCCAA GCACATGACC 
CAAGAGGTGG TCACCCAGTA CTATCGCGCA CCGGAGATTC TGATGGGCGC TCGCCACTAC 
TCGTCGGCGG TGGATGTATG GTCGGTGGGC TGCATTTTTG GCGAGCTGCT GGGCCGTCGC 
ATCCTCTTCC AGGCACAGAA TCCTGTGCAG CAGCTGGAGC TGATTACCGA GCTGCTTGGC 
ACGCCCACCA TGGAGGATAT GCGTCACGCC TGCGAGGGCG CCCGCACCCA CATGTTGCGC 
CGGGCGCCCA AGCCGCCATC CTTCTCGGTG CTCTACACAC TCAGCTCGCA TGCCACGCAC 
GAGGCGGTGC ACTTGCTCTG CCAGATGCTC GTCTTCGATC CGGATAAGCG CATCTCCGTG 
ACAGATGCCC TGGCGCATCC GTATCTGGAT GAGGGGCGTC TGCGCTATCA CTCGTGCATG 
TGCAAATGCT GCTTCACTAC CTCAGCCGGA ATGCGTCAGT ATACGGCTGA TTTTGAGCCC 
TCCGCCGGCC AGCCCTTCGA TGATCTGTGG GAACGTAAGC TGACATCCGT GCAGCAGGTC 
AAAGAGGAGA TGCACAAGTT CATTGCCGAG CAGCTGCAAA CGGGTCGTGT GCCGCTGTGC 
ATCAATCCAC AGAGTGCAGC CTTCAAGAGT TTCGCCAGTT CGACTGTGGC ACATCCTTCG 
GAGCTCCCGC CTTCGCCTCA CCAATGGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261555.1
CDS	719..2011
Translation	MSVSMSLVQGGAAGGAPQGASAILAAAAPYYQPPAVPQDVQPDRPIGYGAFGVVWAVTDPRDGRRVALKKLPNVFQSLVSSKRVFRELKMLCFFKHENVLSALDILQPPHLDFFQEIYVITELLQSDLHKIIVSPQHLSADHIKVFLYQILRGLKYLHSARILHRDIKPGNLLVNSNCVLKICDFGLARVEEPDQAKHMTQEVVTQYYRAPEILMGARHYSSAVDVWSVGCIFGELLGRRILFQAQNPVQQLELITELLGTPTMEDMRHACEGARTHMLRRAPKPPSFSVLYTLSSHATHEAVHLLCQMLVFDPDKRISVTDALAHPYLDEGRLRYHSCMCKCCFTTSAGMRQYTADFEPSAGQPFDDLWERKLTSVQQVKEEMHKFIAEQLQTGRVPLCINPQSAAFKSFASSTVAHPSELPPSPHQWE

Target ORF information:

RefSeq Version	NM_001274626.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nemo (nmo), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274626.1

ORF Insert Sequence:

ATGAGCGTTT CGATGTCACT GGTACAGGGT GGTGCTGCCG GTGGTGCGCC ACAGGGCGCC 
AGCGCAATCT TGGCCGCAGC GGCTCCCTAC TATCAACCGC CGGCTGTTCC CCAGGATGTT 
CAGCCGGATC GTCCTATCGG CTATGGAGCA TTCGGAGTTG TCTGGGCCGT CACAGATCCT 
CGCGATGGAC GACGAGTGGC ATTGAAGAAG CTGCCAAATG TGTTCCAATC GTTGGTCTCA 
TCGAAGCGCG TTTTTCGCGA GCTGAAAATG CTGTGCTTCT TCAAGCATGA AAATGTTCTT 
TCAGCTCTGG ACATTCTACA GCCACCGCAT TTGGATTTCT TTCAAGAAAT CTATGTGATA 
ACGGAGCTGC TGCAATCGGA TCTGCACAAG ATAATCGTGT CGCCACAGCA CCTGTCCGCC 
GACCACATCA AGGTGTTCCT GTACCAGATC CTGCGCGGCC TCAAATATCT GCACTCGGCC 
CGCATCCTGC ATCGCGACAT CAAGCCGGGC AATCTGCTGG TCAACTCGAA TTGCGTGCTC 
AAGATCTGTG ACTTTGGATT GGCCCGCGTC GAGGAGCCGG ATCAGGCCAA GCACATGACC 
CAAGAGGTGG TCACCCAGTA CTATCGCGCA CCGGAGATTC TGATGGGCGC TCGCCACTAC 
TCGTCGGCGG TGGATGTATG GTCGGTGGGC TGCATTTTTG GCGAGCTGCT GGGCCGTCGC 
ATCCTCTTCC AGGCACAGAA TCCTGTGCAG CAGCTGGAGC TGATTACCGA GCTGCTTGGC 
ACGCCCACCA TGGAGGATAT GCGTCACGCC TGCGAGGGCG CCCGCACCCA CATGTTGCGC 
CGGGCGCCCA AGCCGCCATC CTTCTCGGTG CTCTACACAC TCAGCTCGCA TGCCACGCAC 
GAGGCGGTGC ACTTGCTCTG CCAGATGCTC GTCTTCGATC CGGATAAGCG CATCTCCGTG 
ACAGATGCCC TGGCGCATCC GTATCTGGAT GAGGGGCGTC TGCGCTATCA CTCGTGCATG 
TGCAAATGCT GCTTCACTAC CTCAGCCGGA ATGCGTCAGT ATACGGCTGA TTTTGAGCCC 
TCCGCCGGCC AGCCCTTCGA TGATCTGTGG GAACGTAAGC TGACATCCGT GCAGCAGGTC 
AAAGAGGAGA TGCACAAGTT CATTGCCGAG CAGCTGCAAA CGGGTCGTGT GCCGCTGTGC 
ATCAATCCAC AGAGTGCAGC CTTCAAGAGT TTCGCCAGTT CGACTGTGGC ACATCCTTCG 
GAGCTCCCGC CTTCGCCTCA CCAATGGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nmo ( NM_168249.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729317.1 Drosophila melanogaster nemo (nmo), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa17856
Clone ID Related Accession (Same CDS sequence)	NM_001274626.1 , NM_168249.3 , NM_168248.3 , NM_168250.3
Accession Version	NM_168249.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nemo, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168249.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGTTT CGATGTCACT GGTACAGGGT GGTGCTGCCG GTGGTGCGCC ACAGGGCGCC 
AGCGCAATCT TGGCCGCAGC GGCTCCCTAC TATCAACCGC CGGCTGTTCC CCAGGATGTT 
CAGCCGGATC GTCCTATCGG CTATGGAGCA TTCGGAGTTG TCTGGGCCGT CACAGATCCT 
CGCGATGGAC GACGAGTGGC ATTGAAGAAG CTGCCAAATG TGTTCCAATC GTTGGTCTCA 
TCGAAGCGCG TTTTTCGCGA GCTGAAAATG CTGTGCTTCT TCAAGCATGA AAATGTTCTT 
TCAGCTCTGG ACATTCTACA GCCACCGCAT TTGGATTTCT TTCAAGAAAT CTATGTGATA 
ACGGAGCTGC TGCAATCGGA TCTGCACAAG ATAATCGTGT CGCCACAGCA CCTGTCCGCC 
GACCACATCA AGGTGTTCCT GTACCAGATC CTGCGCGGCC TCAAATATCT GCACTCGGCC 
CGCATCCTGC ATCGCGACAT CAAGCCGGGC AATCTGCTGG TCAACTCGAA TTGCGTGCTC 
AAGATCTGTG ACTTTGGATT GGCCCGCGTC GAGGAGCCGG ATCAGGCCAA GCACATGACC 
CAAGAGGTGG TCACCCAGTA CTATCGCGCA CCGGAGATTC TGATGGGCGC TCGCCACTAC 
TCGTCGGCGG TGGATGTATG GTCGGTGGGC TGCATTTTTG GCGAGCTGCT GGGCCGTCGC 
ATCCTCTTCC AGGCACAGAA TCCTGTGCAG CAGCTGGAGC TGATTACCGA GCTGCTTGGC 
ACGCCCACCA TGGAGGATAT GCGTCACGCC TGCGAGGGCG CCCGCACCCA CATGTTGCGC 
CGGGCGCCCA AGCCGCCATC CTTCTCGGTG CTCTACACAC TCAGCTCGCA TGCCACGCAC 
GAGGCGGTGC ACTTGCTCTG CCAGATGCTC GTCTTCGATC CGGATAAGCG CATCTCCGTG 
ACAGATGCCC TGGCGCATCC GTATCTGGAT GAGGGGCGTC TGCGCTATCA CTCGTGCATG 
TGCAAATGCT GCTTCACTAC CTCAGCCGGA ATGCGTCAGT ATACGGCTGA TTTTGAGCCC 
TCCGCCGGCC AGCCCTTCGA TGATCTGTGG GAACGTAAGC TGACATCCGT GCAGCAGGTC 
AAAGAGGAGA TGCACAAGTT CATTGCCGAG CAGCTGCAAA CGGGTCGTGT GCCGCTGTGC 
ATCAATCCAC AGAGTGCAGC CTTCAAGAGT TTCGCCAGTT CGACTGTGGC ACATCCTTCG 
GAGCTCCCGC CTTCGCCTCA CCAATGGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729317.1
CDS	1055..2347
Translation	MSVSMSLVQGGAAGGAPQGASAILAAAAPYYQPPAVPQDVQPDRPIGYGAFGVVWAVTDPRDGRRVALKKLPNVFQSLVSSKRVFRELKMLCFFKHENVLSALDILQPPHLDFFQEIYVITELLQSDLHKIIVSPQHLSADHIKVFLYQILRGLKYLHSARILHRDIKPGNLLVNSNCVLKICDFGLARVEEPDQAKHMTQEVVTQYYRAPEILMGARHYSSAVDVWSVGCIFGELLGRRILFQAQNPVQQLELITELLGTPTMEDMRHACEGARTHMLRRAPKPPSFSVLYTLSSHATHEAVHLLCQMLVFDPDKRISVTDALAHPYLDEGRLRYHSCMCKCCFTTSAGMRQYTADFEPSAGQPFDDLWERKLTSVQQVKEEMHKFIAEQLQTGRVPLCINPQSAAFKSFASSTVAHPSELPPSPHQWE

Target ORF information:

RefSeq Version	NM_168249.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nemo (nmo), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168249.3

ORF Insert Sequence:

ATGAGCGTTT CGATGTCACT GGTACAGGGT GGTGCTGCCG GTGGTGCGCC ACAGGGCGCC 
AGCGCAATCT TGGCCGCAGC GGCTCCCTAC TATCAACCGC CGGCTGTTCC CCAGGATGTT 
CAGCCGGATC GTCCTATCGG CTATGGAGCA TTCGGAGTTG TCTGGGCCGT CACAGATCCT 
CGCGATGGAC GACGAGTGGC ATTGAAGAAG CTGCCAAATG TGTTCCAATC GTTGGTCTCA 
TCGAAGCGCG TTTTTCGCGA GCTGAAAATG CTGTGCTTCT TCAAGCATGA AAATGTTCTT 
TCAGCTCTGG ACATTCTACA GCCACCGCAT TTGGATTTCT TTCAAGAAAT CTATGTGATA 
ACGGAGCTGC TGCAATCGGA TCTGCACAAG ATAATCGTGT CGCCACAGCA CCTGTCCGCC 
GACCACATCA AGGTGTTCCT GTACCAGATC CTGCGCGGCC TCAAATATCT GCACTCGGCC 
CGCATCCTGC ATCGCGACAT CAAGCCGGGC AATCTGCTGG TCAACTCGAA TTGCGTGCTC 
AAGATCTGTG ACTTTGGATT GGCCCGCGTC GAGGAGCCGG ATCAGGCCAA GCACATGACC 
CAAGAGGTGG TCACCCAGTA CTATCGCGCA CCGGAGATTC TGATGGGCGC TCGCCACTAC 
TCGTCGGCGG TGGATGTATG GTCGGTGGGC TGCATTTTTG GCGAGCTGCT GGGCCGTCGC 
ATCCTCTTCC AGGCACAGAA TCCTGTGCAG CAGCTGGAGC TGATTACCGA GCTGCTTGGC 
ACGCCCACCA TGGAGGATAT GCGTCACGCC TGCGAGGGCG CCCGCACCCA CATGTTGCGC 
CGGGCGCCCA AGCCGCCATC CTTCTCGGTG CTCTACACAC TCAGCTCGCA TGCCACGCAC 
GAGGCGGTGC ACTTGCTCTG CCAGATGCTC GTCTTCGATC CGGATAAGCG CATCTCCGTG 
ACAGATGCCC TGGCGCATCC GTATCTGGAT GAGGGGCGTC TGCGCTATCA CTCGTGCATG 
TGCAAATGCT GCTTCACTAC CTCAGCCGGA ATGCGTCAGT ATACGGCTGA TTTTGAGCCC 
TCCGCCGGCC AGCCCTTCGA TGATCTGTGG GAACGTAAGC TGACATCCGT GCAGCAGGTC 
AAAGAGGAGA TGCACAAGTT CATTGCCGAG CAGCTGCAAA CGGGTCGTGT GCCGCTGTGC 
ATCAATCCAC AGAGTGCAGC CTTCAAGAGT TTCGCCAGTT CGACTGTGGC ACATCCTTCG 
GAGCTCCCGC CTTCGCCTCA CCAATGGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nmo ( NM_168248.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729316.1 Drosophila melanogaster nemo (nmo), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa17856
Clone ID Related Accession (Same CDS sequence)	NM_001274626.1 , NM_168249.3 , NM_168248.3 , NM_168250.3
Accession Version	NM_168248.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nemo, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168248.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGTTT CGATGTCACT GGTACAGGGT GGTGCTGCCG GTGGTGCGCC ACAGGGCGCC 
AGCGCAATCT TGGCCGCAGC GGCTCCCTAC TATCAACCGC CGGCTGTTCC CCAGGATGTT 
CAGCCGGATC GTCCTATCGG CTATGGAGCA TTCGGAGTTG TCTGGGCCGT CACAGATCCT 
CGCGATGGAC GACGAGTGGC ATTGAAGAAG CTGCCAAATG TGTTCCAATC GTTGGTCTCA 
TCGAAGCGCG TTTTTCGCGA GCTGAAAATG CTGTGCTTCT TCAAGCATGA AAATGTTCTT 
TCAGCTCTGG ACATTCTACA GCCACCGCAT TTGGATTTCT TTCAAGAAAT CTATGTGATA 
ACGGAGCTGC TGCAATCGGA TCTGCACAAG ATAATCGTGT CGCCACAGCA CCTGTCCGCC 
GACCACATCA AGGTGTTCCT GTACCAGATC CTGCGCGGCC TCAAATATCT GCACTCGGCC 
CGCATCCTGC ATCGCGACAT CAAGCCGGGC AATCTGCTGG TCAACTCGAA TTGCGTGCTC 
AAGATCTGTG ACTTTGGATT GGCCCGCGTC GAGGAGCCGG ATCAGGCCAA GCACATGACC 
CAAGAGGTGG TCACCCAGTA CTATCGCGCA CCGGAGATTC TGATGGGCGC TCGCCACTAC 
TCGTCGGCGG TGGATGTATG GTCGGTGGGC TGCATTTTTG GCGAGCTGCT GGGCCGTCGC 
ATCCTCTTCC AGGCACAGAA TCCTGTGCAG CAGCTGGAGC TGATTACCGA GCTGCTTGGC 
ACGCCCACCA TGGAGGATAT GCGTCACGCC TGCGAGGGCG CCCGCACCCA CATGTTGCGC 
CGGGCGCCCA AGCCGCCATC CTTCTCGGTG CTCTACACAC TCAGCTCGCA TGCCACGCAC 
GAGGCGGTGC ACTTGCTCTG CCAGATGCTC GTCTTCGATC CGGATAAGCG CATCTCCGTG 
ACAGATGCCC TGGCGCATCC GTATCTGGAT GAGGGGCGTC TGCGCTATCA CTCGTGCATG 
TGCAAATGCT GCTTCACTAC CTCAGCCGGA ATGCGTCAGT ATACGGCTGA TTTTGAGCCC 
TCCGCCGGCC AGCCCTTCGA TGATCTGTGG GAACGTAAGC TGACATCCGT GCAGCAGGTC 
AAAGAGGAGA TGCACAAGTT CATTGCCGAG CAGCTGCAAA CGGGTCGTGT GCCGCTGTGC 
ATCAATCCAC AGAGTGCAGC CTTCAAGAGT TTCGCCAGTT CGACTGTGGC ACATCCTTCG 
GAGCTCCCGC CTTCGCCTCA CCAATGGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729316.1
CDS	719..2011
Translation	MSVSMSLVQGGAAGGAPQGASAILAAAAPYYQPPAVPQDVQPDRPIGYGAFGVVWAVTDPRDGRRVALKKLPNVFQSLVSSKRVFRELKMLCFFKHENVLSALDILQPPHLDFFQEIYVITELLQSDLHKIIVSPQHLSADHIKVFLYQILRGLKYLHSARILHRDIKPGNLLVNSNCVLKICDFGLARVEEPDQAKHMTQEVVTQYYRAPEILMGARHYSSAVDVWSVGCIFGELLGRRILFQAQNPVQQLELITELLGTPTMEDMRHACEGARTHMLRRAPKPPSFSVLYTLSSHATHEAVHLLCQMLVFDPDKRISVTDALAHPYLDEGRLRYHSCMCKCCFTTSAGMRQYTADFEPSAGQPFDDLWERKLTSVQQVKEEMHKFIAEQLQTGRVPLCINPQSAAFKSFASSTVAHPSELPPSPHQWE

Target ORF information:

RefSeq Version	NM_168248.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nemo (nmo), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168248.3

ORF Insert Sequence:

ATGAGCGTTT CGATGTCACT GGTACAGGGT GGTGCTGCCG GTGGTGCGCC ACAGGGCGCC 
AGCGCAATCT TGGCCGCAGC GGCTCCCTAC TATCAACCGC CGGCTGTTCC CCAGGATGTT 
CAGCCGGATC GTCCTATCGG CTATGGAGCA TTCGGAGTTG TCTGGGCCGT CACAGATCCT 
CGCGATGGAC GACGAGTGGC ATTGAAGAAG CTGCCAAATG TGTTCCAATC GTTGGTCTCA 
TCGAAGCGCG TTTTTCGCGA GCTGAAAATG CTGTGCTTCT TCAAGCATGA AAATGTTCTT 
TCAGCTCTGG ACATTCTACA GCCACCGCAT TTGGATTTCT TTCAAGAAAT CTATGTGATA 
ACGGAGCTGC TGCAATCGGA TCTGCACAAG ATAATCGTGT CGCCACAGCA CCTGTCCGCC 
GACCACATCA AGGTGTTCCT GTACCAGATC CTGCGCGGCC TCAAATATCT GCACTCGGCC 
CGCATCCTGC ATCGCGACAT CAAGCCGGGC AATCTGCTGG TCAACTCGAA TTGCGTGCTC 
AAGATCTGTG ACTTTGGATT GGCCCGCGTC GAGGAGCCGG ATCAGGCCAA GCACATGACC 
CAAGAGGTGG TCACCCAGTA CTATCGCGCA CCGGAGATTC TGATGGGCGC TCGCCACTAC 
TCGTCGGCGG TGGATGTATG GTCGGTGGGC TGCATTTTTG GCGAGCTGCT GGGCCGTCGC 
ATCCTCTTCC AGGCACAGAA TCCTGTGCAG CAGCTGGAGC TGATTACCGA GCTGCTTGGC 
ACGCCCACCA TGGAGGATAT GCGTCACGCC TGCGAGGGCG CCCGCACCCA CATGTTGCGC 
CGGGCGCCCA AGCCGCCATC CTTCTCGGTG CTCTACACAC TCAGCTCGCA TGCCACGCAC 
GAGGCGGTGC ACTTGCTCTG CCAGATGCTC GTCTTCGATC CGGATAAGCG CATCTCCGTG 
ACAGATGCCC TGGCGCATCC GTATCTGGAT GAGGGGCGTC TGCGCTATCA CTCGTGCATG 
TGCAAATGCT GCTTCACTAC CTCAGCCGGA ATGCGTCAGT ATACGGCTGA TTTTGAGCCC 
TCCGCCGGCC AGCCCTTCGA TGATCTGTGG GAACGTAAGC TGACATCCGT GCAGCAGGTC 
AAAGAGGAGA TGCACAAGTT CATTGCCGAG CAGCTGCAAA CGGGTCGTGT GCCGCTGTGC 
ATCAATCCAC AGAGTGCAGC CTTCAAGAGT TTCGCCAGTT CGACTGTGGC ACATCCTTCG 
GAGCTCCCGC CTTCGCCTCA CCAATGGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nmo ( NM_168250.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729318.1 Drosophila melanogaster nemo (nmo), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa17856
Clone ID Related Accession (Same CDS sequence)	NM_001274626.1 , NM_168249.3 , NM_168248.3 , NM_168250.3
Accession Version	NM_168250.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nemo, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168250.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGTTT CGATGTCACT GGTACAGGGT GGTGCTGCCG GTGGTGCGCC ACAGGGCGCC 
AGCGCAATCT TGGCCGCAGC GGCTCCCTAC TATCAACCGC CGGCTGTTCC CCAGGATGTT 
CAGCCGGATC GTCCTATCGG CTATGGAGCA TTCGGAGTTG TCTGGGCCGT CACAGATCCT 
CGCGATGGAC GACGAGTGGC ATTGAAGAAG CTGCCAAATG TGTTCCAATC GTTGGTCTCA 
TCGAAGCGCG TTTTTCGCGA GCTGAAAATG CTGTGCTTCT TCAAGCATGA AAATGTTCTT 
TCAGCTCTGG ACATTCTACA GCCACCGCAT TTGGATTTCT TTCAAGAAAT CTATGTGATA 
ACGGAGCTGC TGCAATCGGA TCTGCACAAG ATAATCGTGT CGCCACAGCA CCTGTCCGCC 
GACCACATCA AGGTGTTCCT GTACCAGATC CTGCGCGGCC TCAAATATCT GCACTCGGCC 
CGCATCCTGC ATCGCGACAT CAAGCCGGGC AATCTGCTGG TCAACTCGAA TTGCGTGCTC 
AAGATCTGTG ACTTTGGATT GGCCCGCGTC GAGGAGCCGG ATCAGGCCAA GCACATGACC 
CAAGAGGTGG TCACCCAGTA CTATCGCGCA CCGGAGATTC TGATGGGCGC TCGCCACTAC 
TCGTCGGCGG TGGATGTATG GTCGGTGGGC TGCATTTTTG GCGAGCTGCT GGGCCGTCGC 
ATCCTCTTCC AGGCACAGAA TCCTGTGCAG CAGCTGGAGC TGATTACCGA GCTGCTTGGC 
ACGCCCACCA TGGAGGATAT GCGTCACGCC TGCGAGGGCG CCCGCACCCA CATGTTGCGC 
CGGGCGCCCA AGCCGCCATC CTTCTCGGTG CTCTACACAC TCAGCTCGCA TGCCACGCAC 
GAGGCGGTGC ACTTGCTCTG CCAGATGCTC GTCTTCGATC CGGATAAGCG CATCTCCGTG 
ACAGATGCCC TGGCGCATCC GTATCTGGAT GAGGGGCGTC TGCGCTATCA CTCGTGCATG 
TGCAAATGCT GCTTCACTAC CTCAGCCGGA ATGCGTCAGT ATACGGCTGA TTTTGAGCCC 
TCCGCCGGCC AGCCCTTCGA TGATCTGTGG GAACGTAAGC TGACATCCGT GCAGCAGGTC 
AAAGAGGAGA TGCACAAGTT CATTGCCGAG CAGCTGCAAA CGGGTCGTGT GCCGCTGTGC 
ATCAATCCAC AGAGTGCAGC CTTCAAGAGT TTCGCCAGTT CGACTGTGGC ACATCCTTCG 
GAGCTCCCGC CTTCGCCTCA CCAATGGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729318.1
CDS	548..1840
Translation	MSVSMSLVQGGAAGGAPQGASAILAAAAPYYQPPAVPQDVQPDRPIGYGAFGVVWAVTDPRDGRRVALKKLPNVFQSLVSSKRVFRELKMLCFFKHENVLSALDILQPPHLDFFQEIYVITELLQSDLHKIIVSPQHLSADHIKVFLYQILRGLKYLHSARILHRDIKPGNLLVNSNCVLKICDFGLARVEEPDQAKHMTQEVVTQYYRAPEILMGARHYSSAVDVWSVGCIFGELLGRRILFQAQNPVQQLELITELLGTPTMEDMRHACEGARTHMLRRAPKPPSFSVLYTLSSHATHEAVHLLCQMLVFDPDKRISVTDALAHPYLDEGRLRYHSCMCKCCFTTSAGMRQYTADFEPSAGQPFDDLWERKLTSVQQVKEEMHKFIAEQLQTGRVPLCINPQSAAFKSFASSTVAHPSELPPSPHQWE

Target ORF information:

RefSeq Version	NM_168250.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nemo (nmo), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168250.3

ORF Insert Sequence:

ATGAGCGTTT CGATGTCACT GGTACAGGGT GGTGCTGCCG GTGGTGCGCC ACAGGGCGCC 
AGCGCAATCT TGGCCGCAGC GGCTCCCTAC TATCAACCGC CGGCTGTTCC CCAGGATGTT 
CAGCCGGATC GTCCTATCGG CTATGGAGCA TTCGGAGTTG TCTGGGCCGT CACAGATCCT 
CGCGATGGAC GACGAGTGGC ATTGAAGAAG CTGCCAAATG TGTTCCAATC GTTGGTCTCA 
TCGAAGCGCG TTTTTCGCGA GCTGAAAATG CTGTGCTTCT TCAAGCATGA AAATGTTCTT 
TCAGCTCTGG ACATTCTACA GCCACCGCAT TTGGATTTCT TTCAAGAAAT CTATGTGATA 
ACGGAGCTGC TGCAATCGGA TCTGCACAAG ATAATCGTGT CGCCACAGCA CCTGTCCGCC 
GACCACATCA AGGTGTTCCT GTACCAGATC CTGCGCGGCC TCAAATATCT GCACTCGGCC 
CGCATCCTGC ATCGCGACAT CAAGCCGGGC AATCTGCTGG TCAACTCGAA TTGCGTGCTC 
AAGATCTGTG ACTTTGGATT GGCCCGCGTC GAGGAGCCGG ATCAGGCCAA GCACATGACC 
CAAGAGGTGG TCACCCAGTA CTATCGCGCA CCGGAGATTC TGATGGGCGC TCGCCACTAC 
TCGTCGGCGG TGGATGTATG GTCGGTGGGC TGCATTTTTG GCGAGCTGCT GGGCCGTCGC 
ATCCTCTTCC AGGCACAGAA TCCTGTGCAG CAGCTGGAGC TGATTACCGA GCTGCTTGGC 
ACGCCCACCA TGGAGGATAT GCGTCACGCC TGCGAGGGCG CCCGCACCCA CATGTTGCGC 
CGGGCGCCCA AGCCGCCATC CTTCTCGGTG CTCTACACAC TCAGCTCGCA TGCCACGCAC 
GAGGCGGTGC ACTTGCTCTG CCAGATGCTC GTCTTCGATC CGGATAAGCG CATCTCCGTG 
ACAGATGCCC TGGCGCATCC GTATCTGGAT GAGGGGCGTC TGCGCTATCA CTCGTGCATG 
TGCAAATGCT GCTTCACTAC CTCAGCCGGA ATGCGTCAGT ATACGGCTGA TTTTGAGCCC 
TCCGCCGGCC AGCCCTTCGA TGATCTGTGG GAACGTAAGC TGACATCCGT GCAGCAGGTC 
AAAGAGGAGA TGCACAAGTT CATTGCCGAG CAGCTGCAAA CGGGTCGTGT GCCGCTGTGC 
ATCAATCCAC AGAGTGCAGC CTTCAAGAGT TTCGCCAGTT CGACTGTGGC ACATCCTTCG 
GAGCTCCCGC CTTCGCCTCA CCAATGGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nmo ( NM_001370048.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001356976.1 Drosophila melanogaster nemo (nmo), transcript variant I, mRNA.

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CloneID	OFa87761
Clone ID Related Accession (Same CDS sequence)	NM_001370048.1
Accession Version	NM_001370048.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nemo, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGTTT CGATGTCACT GGTACAGGGT GGTGCTGCCG GTGGTGCGCC ACAGGGCGCC 
AGCGCAATCT TGGCCGCAGC GGCTCCCTAC TATCAACCGC CGGCTGTTCC CCAGGATGTT 
CAGCCGGATC GTCCTATCGG CTATGGAGCA TTCGGAGTTG TCTGGGCCGT CACAGATCCT 
CGCGATGGAC GACGAGTGGC ATTGAAGAAG CTGCCAAATG TGTTCCAATC GTTGGTCTCA 
TCGAAGCGCG TTTTTCGCGA GCTGAAAATG CTGTGCTTCT TCAAGCATGA AAATGTTCTT 
TCAGCTCTGG ACATTCTACA GCCACCGCAT TTGGATTTCT TTCAAGAAAT CTATGTGATA 
ACGGAGCTGC TGCAATCGGA TCTGCACAAG ATAATCGTGT CGCCACAGCA CCTGTCCGCC 
GACCACATCA AGGTGTTCCT GTACCAGATC CTGCGCGGCC TCAAATATCT GCACTCGGCC 
CGCATCCTGC ATCGCGACAT CAAGCCGGGC AATCTGCTGG TCAACTCGAA TTGCGTGCTC 
AAGATCTGTG ACTTTGGATT GGCCCGCGTC GAGGAGCCGG ATCAGGCCAA GCACATGACC 
CAAGAGGTGG TCACCCAGTA CTATCGCGCA CCGGAGATTC TGATGGGCGC TCGCCACTAC 
TCGTCGGCGG TGGATGTATG GTCGGTGGGC TGCATTTTTG GCGAGCTGCT GGGCCGTCGC 
ATCCTCTTCC AGGCACAGAA TCCTGTGCAG CAGCTGGAGC TGATTACCGA GCTGCTTGGC 
ACGCCCACCA TGGAGGATAT GCGTCACGCC TGCGAGGGCG CCCGCACCCA CATGTTGCGC 
CGGGCGCCCA AGCCGCCATC CTTCTCGGTG CTCTACACAC TCAGCTCGCA TGCCACGCAC 
GAGGCGGTGC ACTTGCTCTG CCAGATGCTC GTCTTCGATC CGGATAAGCG CATCTCCGTG 
ACAGATGCCC TGGCGCATCC GTATCTGGAT GAGGGGCGTC TGCGCTATCA CTCGTGCATG 
TGCAAATGCT GCTTCACTAC CTCAGCCGGA ATGCGTCAGT ATACGGCTGA TTTTGAGCCC 
TCCGCCGGCC AGCCCTTCGA TGATCTGTGG GAACGTAAGC TGACATCCGT GCAGCAGGTC 
AAAGAGGAGA TGCACAAGTT CATTGCCGAG CAGCTGCAAA CGGGTCGTGT GCCGCTGTGC 
ATCAATCCAC AGAGTGCAGC CTTCAAGAGT TTCGCCAGTT CGACTGTGGC ACATCCTTCG 
GAGCTCCCGC CTTCGCCTCA CCAATGGGAA TGACGGCAAA ATGAGGCGAT TGCTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356976.1
CDS	548..1867
Translation	MSVSMSLVQGGAAGGAPQGASAILAAAAPYYQPPAVPQDVQPDRPIGYGAFGVVWAVTDPRDGRRVALKKLPNVFQSLVSSKRVFRELKMLCFFKHENVLSALDILQPPHLDFFQEIYVITELLQSDLHKIIVSPQHLSADHIKVFLYQILRGLKYLHSARILHRDIKPGNLLVNSNCVLKICDFGLARVEEPDQAKHMTQEVVTQYYRAPEILMGARHYSSAVDVWSVGCIFGELLGRRILFQAQNPVQQLELITELLGTPTMEDMRHACEGARTHMLRRAPKPPSFSVLYTLSSHATHEAVHLLCQMLVFDPDKRISVTDALAHPYLDEGRLRYHSCMCKCCFTTSAGMRQYTADFEPSAGQPFDDLWERKLTSVQQVKEEMHKFIAEQLQTGRVPLCINPQSAAFKSFASSTVAHPSELPPSPHQWE*RQNEAIAA

Target ORF information:

RefSeq Version	NM_001370048.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nemo (nmo), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370048.1

ORF Insert Sequence:

ATGAGCGTTT CGATGTCACT GGTACAGGGT GGTGCTGCCG GTGGTGCGCC ACAGGGCGCC 
AGCGCAATCT TGGCCGCAGC GGCTCCCTAC TATCAACCGC CGGCTGTTCC CCAGGATGTT 
CAGCCGGATC GTCCTATCGG CTATGGAGCA TTCGGAGTTG TCTGGGCCGT CACAGATCCT 
CGCGATGGAC GACGAGTGGC ATTGAAGAAG CTGCCAAATG TGTTCCAATC GTTGGTCTCA 
TCGAAGCGCG TTTTTCGCGA GCTGAAAATG CTGTGCTTCT TCAAGCATGA AAATGTTCTT 
TCAGCTCTGG ACATTCTACA GCCACCGCAT TTGGATTTCT TTCAAGAAAT CTATGTGATA 
ACGGAGCTGC TGCAATCGGA TCTGCACAAG ATAATCGTGT CGCCACAGCA CCTGTCCGCC 
GACCACATCA AGGTGTTCCT GTACCAGATC CTGCGCGGCC TCAAATATCT GCACTCGGCC 
CGCATCCTGC ATCGCGACAT CAAGCCGGGC AATCTGCTGG TCAACTCGAA TTGCGTGCTC 
AAGATCTGTG ACTTTGGATT GGCCCGCGTC GAGGAGCCGG ATCAGGCCAA GCACATGACC 
CAAGAGGTGG TCACCCAGTA CTATCGCGCA CCGGAGATTC TGATGGGCGC TCGCCACTAC 
TCGTCGGCGG TGGATGTATG GTCGGTGGGC TGCATTTTTG GCGAGCTGCT GGGCCGTCGC 
ATCCTCTTCC AGGCACAGAA TCCTGTGCAG CAGCTGGAGC TGATTACCGA GCTGCTTGGC 
ACGCCCACCA TGGAGGATAT GCGTCACGCC TGCGAGGGCG CCCGCACCCA CATGTTGCGC 
CGGGCGCCCA AGCCGCCATC CTTCTCGGTG CTCTACACAC TCAGCTCGCA TGCCACGCAC 
GAGGCGGTGC ACTTGCTCTG CCAGATGCTC GTCTTCGATC CGGATAAGCG CATCTCCGTG 
ACAGATGCCC TGGCGCATCC GTATCTGGAT GAGGGGCGTC TGCGCTATCA CTCGTGCATG 
TGCAAATGCT GCTTCACTAC CTCAGCCGGA ATGCGTCAGT ATACGGCTGA TTTTGAGCCC 
TCCGCCGGCC AGCCCTTCGA TGATCTGTGG GAACGTAAGC TGACATCCGT GCAGCAGGTC 
AAAGAGGAGA TGCACAAGTT CATTGCCGAG CAGCTGCAAA CGGGTCGTGT GCCGCTGTGC 
ATCAATCCAC AGAGTGCAGC CTTCAAGAGT TTCGCCAGTT CGACTGTGGC ACATCCTTCG 
GAGCTCCCGC CTTCGCCTCA CCAATGGGAA TGACGGCAAA ATGAGGCGAT TGCTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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nmo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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