Clk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Clk
Entrez Gene ID	38872
Full Name	Clock
Synonyms	CG7391,CLK,CLOCK,Dmel\CG7391,Jerk,Jrk,PAS1,bHLHe10,clk,clock,dCLK,dCLK/JRK,dCLOCK,dClck,dClk,dClock,dclk,jrk
General protein information	Preferred Names Clock Names CG7391 gene product from transcript CG7391-RA CG7391-PA CG7391-PD CG7391-PF CG7391-PG CG7391-PH CLOCK Clk-PA Clk-PD Clk-PF Clk-PG Clk-PH clock dClock
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66A12-66A12

mRNA and Protein(s)

mRNA	Protein	Name
NM_001014574.2	NP_001014574.1	clock, isoform F
NM_001014576.2	NP_001014576.1	clock, isoform D
NM_079240.3	NP_523964.2	clock, isoform A
NM_206299.2	NP_996021.2	clock, isoform H
NM_170628.2	NP_729294.2	clock, isoform G

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ClkAR mutants showed significantly faster age-related locomotor deficits. Accelerated locomotor decline of the ClkAR mutant required expression of the PDF receptor and correlated to an apparent loss of dopaminergic neurons in the brain
Title: Drosophila Clock Is Required in Brain Pacemaker Neurons to Prevent Premature Locomotor Aging Independently of Its Circadian Function.

Here we demonstrate that the transcription factor CLOCKWORK ORANGE (CWO) antagonizes CLK-CYC E-box binding, thus enhancing the removal of CLK-CYC from E-boxes to maintain transcriptional repression. This process requires PER, which suggests that PER-TIM and CWO cooperate to maintain a transcriptionally repressed state by removing CLK-CYC from E-boxes
Title: CLOCKWORK ORANGE Enhances PERIOD Mediated Rhythms in Transcriptional Repression by Antagonizing E-box Binding by CLOCK-CYCLE.

these results demonstrate a key role of Clk post-transcriptional control in stabilizing circadian transcription.
Title: Clk post-transcriptional control denoises circadian transcription both temporally and spatially.

Our findings suggest a novel role for clock protein phosphorylation in governing the relative strengths of entraining modalities by adjusting the dynamics of circadian gene expression.
Title: Phosphorylation of a central clock transcription factor is required for thermal but not photic entrainment.

These results demonstrate that CLK phosphorylation influences the circadian period by regulating CLK activity and progression through the feedback loop.
Title: Phosphorylation of the transcription activator CLOCK regulates progression through a ? 24-h feedback loop to influence the circadian period in Drosophila.

The following Clk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Clk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17833	NM_079240.3 Latest version!	Drosophila melanogaster clock, transcript variant A (Clk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa17834	NM_170628.2 Latest version!	Drosophila melanogaster clock, transcript variant G (Clk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa17834	NM_206299.2 Latest version!	Drosophila melanogaster clock, transcript variant H (Clk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa17835	NM_001014574.2 Latest version!	Drosophila melanogaster clock, transcript variant F (Clk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa17836	NM_001014576.2 Latest version!	Drosophila melanogaster clock, transcript variant D (Clk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Clk ( NM_079240.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523964.2 Drosophila melanogaster clock, transcript variant A (Clk), mRNA.

Price & Availability↑

CloneID	OFa17833
Clone ID Related Accession (Same CDS sequence)	NM_079240.3
Accession Version	NM_079240.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	clock, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079240.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGACG AGAGCGACGA CAAGGATGAT ACAAAAAGGA AATCGCGTAA TCTCAGCGAG 
AAGAAGCGAC GAGATCAGTT CAACTCGCTG GTCAACGATC TCAGCGCCTT GATATCCACA 
TCCAGCAGAA AGATGGATAA GTCCACGGTC CTGAAGTCCA CGATAGCCTT CCTGAAAAAT 
CATAATGAGG CCACCGATCG CTCCAAGGTT TTCGAAATAC AACAGGACTG GAAGCCTGCG 
TTCCTCAGCA ATGACGAATA CACTCACCTC ATGCTGGAGT CTCTCGATGG TTTTATGATG 
GTCTTCAGCA GCATGGGCTC CATCTTTTAC GCCTCGGAAA GTATTACCTC CCAGCTGGGC 
TATCTGCCGC AAGATTTGTA CAACATGACC ATTTATGACC TGGCCTATGA GATGGACCAT 
GAGGCGCTGC TGAATATCTT TATGAATCCC ACACCGGTAA TCGAGCCCAG ACAGACGGAC 
ATAAGTTCCA GTAACCAGAT CACATTCTAC ACTCATTTGA GACGCGGCGG GATGGAGAAA 
GTGGATGCCA ATGCCTACGA GTTGGTCAAA TTCGTGGGCT ACTTTCGTAA CGATACCAAT 
ACTTCGACGG GTTCCAGTTC GGAGGTTTCG AATGGCTCAA ATGGACAACC TGCGGTGCTG 
CCGCGCATCT TCCAGCAGAA TCCCAATGCC GAGGTGGACA AAAAGCTGGT TTTCGTGGGC 
ACTGGACGCG TACAGAATCC CCAACTGATC AGGGAAATGA GCATTATTGA TCCCACAAGT 
AATGAGTTCA CTTCGAAACA CAGCATGGAG TGGAAGTTTT TGTTTCTGGA TCACCGTGCT 
CCTCCGATCA TTGGCTATAT GCCCTTCGAG GTTCTGGGGA CGTCCGGCTA TGATTATTAC 
CACTTTGATG ATCTGGACAG TATTGTGGCG TGCCATGAAG AGCTAAGGCA AACGGGTGAG 
GGCAAGTCTT GCTACTACCG ATTCCTTACG AAGGGCCAAC AGTGGATATG GTTGCAAACG 
GACTACTATG TGAGCTATCA CCAGTTCAAT TCCAAACCAG ACTACGTGGT CTGCACCCAT 
AAGGTTGTCA GCTATGCCGA GGTTCTGAAG GATAGTCGAA AGGAGGGCCA AAAATCGGGA 
AATAGCAACA GCATCACCAA CAATGGGAGC AGTAAGGTCA TAGCCTCCAC GGGAACTAGC 
AGCAAATCCG CATCTGCCAC CACAACACTG AGGGACTTCG AGCTGAGCAG CCAAAATCTG 
GATAGCACAT TGCTCGGAAA TAGTCTGGCC AGTTTGGGCA CTGAAACAGC AGCCACTTCA 
CCCGCCGTGG ACTCGTCGCC CATGTGGTCA GCGTCGGCTG TCCAACCGTC GGGATCATGC 
CAAATAAATC CCCTGAAGAC ATCGCGTCCC GCTTCGAGTT ATGGCAATAT CAGCTCCACA 
GGAATATCGC CGAAGGCCAA ACGAAAGTGC TACTTCTACA ACAACCGGGG GAATGACTCG 
GATTCAACGT CCATGTCGAC GGATTCGGTC ACCAGCCGGC AGTCCATGAT GACGCACGTC 
AGTTCGCAAA GCCAACGGCA GCGATCGCAT CACCGAGAAC ACCATCGAGA AAATCATCAC 
AATCAGTCAC ATCATCACAT GCAGCAACAG CAGCAGCATC AGAACCAGCA GCAGCAACAT 
CAGCAGCACC AGCAACTGCA ACAGCAGCTG CAACATACAG TGGGTACTCC GAAAATGGTG 
CCTCTCCTGC CGATTGCGTC CACACAAATA ATGGCTGGAA ATGCGTGTCA GTTTCCCCAA 
CCCGCCTATC CGCTTGCTAG TCCCCAACTG GTGGCACCGA CATTTCTTGA GCCGCCGCAA 
TATCTCACCG CCATTCCCAT GCAGCCAGTG ATCGCTCCGT TTCCGGTGGC GCCAGTGCTT 
TCCCCACTTC CGGTGCAGTC ACAAACGGAT ATGCTGCCCG ATACGGTGGT TATGACGCCA 
ACACAGAGTC AGTTGCAGGA TCAATTGCAG CGGAAGCACG ACGAACTGCA AAAACTGATC 
CTCCAGCAAC AGAATGAGCT GAGGATCGTA TCAGAGCAGC TCCTCCTATC CCGCTACACA 
TATCTGCAGC CCATGATGTC GATGGGATTC GCACCTGGCA ACATGACAGC AGCAGCGGTG 
GGTAATTTGG GAGCCAGTGG CCAACGTGGA CTGAATTTCA CCGGCAGCAA TGCCGTGCAG 
CCGCAATTCA ATCAGTATGG ATTTGCACTG AACTCGGAGC AGATGCTTAA CCAGCAGGAT 
CAGCAGATGA TGATGCAGCA GCAGCAGAAT CTGCACACGC AGCACCAGCA CAATCTCCAG 
CAGCAACATC AGAGCCACTC ACAGTTGCAG CAACATACCC AGCAGCAACA TCAGCAGCAG 
CAGCAACAAC AGCAACAACA GCAGCAACAG CAGCAGCAGC AACAGCAACA ACAGCAGCAG 
CAACAACAGC AGCAACAGCA GCAGCAACAG TTGCAGCTGC AACAACAAAA TGATATTTTA 
CTGCGTGAGG ATATCGATGA TATAGACGCC TTTCTCAATC TGTCCCCCCT GCACTCATTG 
GGCTCGCAAT CCACCATTAA TCCCTTCAAC TCCAGCAGCA ATAACAACAA TCAAAGCTAC 
AATGGAGGCA GTAATCTGAA TAATGGCAAC CAGAACAACA ACAATCGCAG TTCAAATCCT 
CCGCAAAATA ATAACGAGGA CTCGCTGTTG TCCTGCATGC AGATGGCCAC CGAATCGAGT 
CCTTCGATCA ACTTTCACAT GGGCATCAGC GATGATGGCT CCGAAACGCA GAGCGAGGAC 
AACAAGATGA TGCACACTTC CGGCAGCAAT CTGGTGCAGC AGCAACAGCA GCAGCAGCAA 
CAACAACAAA TCCTGCAGCA GCATCAACAG CAGTCAAATA GCTTCTTCAG TTCGAATCCC 
TTTCTCAACA GTCAGAATCA GAATCAAAAC CAATTGCCAA ACGATCTGGA AATATTGCCA 
TACCAGATGT CCCAAGAGCA ATCACAGAAT CTATTCAACT CGCCACACAC AGCCCCAGGC 
AGTAGTCAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523964.2
CDS	371..3442
Translation	MDDESDDKDDTKRKSRNLSEKKRRDQFNSLVNDLSALISTSSRKMDKSTVLKSTIAFLKNHNEATDRSKVFEIQQDWKPAFLSNDEYTHLMLESLDGFMMVFSSMGSIFYASESITSQLGYLPQDLYNMTIYDLAYEMDHEALLNIFMNPTPVIEPRQTDISSSNQITFYTHLRRGGMEKVDANAYELVKFVGYFRNDTNTSTGSSSEVSNGSNGQPAVLPRIFQQNPNAEVDKKLVFVGTGRVQNPQLIREMSIIDPTSNEFTSKHSMEWKFLFLDHRAPPIIGYMPFEVLGTSGYDYYHFDDLDSIVACHEELRQTGEGKSCYYRFLTKGQQWIWLQTDYYVSYHQFNSKPDYVVCTHKVVSYAEVLKDSRKEGQKSGNSNSITNNGSSKVIASTGTSSKSASATTTLRDFELSSQNLDSTLLGNSLASLGTETAATSPAVDSSPMWSASAVQPSGSCQINPLKTSRPASSYGNISSTGISPKAKRKCYFYNNRGNDSDSTSMSTDSVTSRQSMMTHVSSQSQRQRSHHREHHRENHHNQSHHHMQQQQQHQNQQQQHQQHQQLQQQLQHTVGTPKMVPLLPIASTQIMAGNACQFPQPAYPLASPQLVAPTFLEPPQYLTAIPMQPVIAPFPVAPVLSPLPVQSQTDMLPDTVVMTPTQSQLQDQLQRKHDELQKLILQQQNELRIVSEQLLLSRYTYLQPMMSMGFAPGNMTAAAVGNLGASGQRGLNFTGSNAVQPQFNQYGFALNSEQMLNQQDQQMMMQQQQNLHTQHQHNLQQQHQSHSQLQQHTQQQHQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQLQLQQQNDILLREDIDDIDAFLNLSPLHSLGSQSTINPFNSSSNNNNQSYNGGSNLNNGNQNNNNRSSNPPQNNNEDSLLSCMQMATESSPSINFHMGISDDGSETQSEDNKMMHTSGSNLVQQQQQQQQQQQILQQHQQQSNSFFSSNPFLNSQNQNQNQLPNDLEILPYQMSQEQSQNLFNSPHTAPGSSQ

Target ORF information:

RefSeq Version	NM_079240.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster clock, transcript variant A (Clk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079240.3

ORF Insert Sequence:

ATGGACGACG AGAGCGACGA CAAGGATGAT ACAAAAAGGA AATCGCGTAA TCTCAGCGAG 
AAGAAGCGAC GAGATCAGTT CAACTCGCTG GTCAACGATC TCAGCGCCTT GATATCCACA 
TCCAGCAGAA AGATGGATAA GTCCACGGTC CTGAAGTCCA CGATAGCCTT CCTGAAAAAT 
CATAATGAGG CCACCGATCG CTCCAAGGTT TTCGAAATAC AACAGGACTG GAAGCCTGCG 
TTCCTCAGCA ATGACGAATA CACTCACCTC ATGCTGGAGT CTCTCGATGG TTTTATGATG 
GTCTTCAGCA GCATGGGCTC CATCTTTTAC GCCTCGGAAA GTATTACCTC CCAGCTGGGC 
TATCTGCCGC AAGATTTGTA CAACATGACC ATTTATGACC TGGCCTATGA GATGGACCAT 
GAGGCGCTGC TGAATATCTT TATGAATCCC ACACCGGTAA TCGAGCCCAG ACAGACGGAC 
ATAAGTTCCA GTAACCAGAT CACATTCTAC ACTCATTTGA GACGCGGCGG GATGGAGAAA 
GTGGATGCCA ATGCCTACGA GTTGGTCAAA TTCGTGGGCT ACTTTCGTAA CGATACCAAT 
ACTTCGACGG GTTCCAGTTC GGAGGTTTCG AATGGCTCAA ATGGACAACC TGCGGTGCTG 
CCGCGCATCT TCCAGCAGAA TCCCAATGCC GAGGTGGACA AAAAGCTGGT TTTCGTGGGC 
ACTGGACGCG TACAGAATCC CCAACTGATC AGGGAAATGA GCATTATTGA TCCCACAAGT 
AATGAGTTCA CTTCGAAACA CAGCATGGAG TGGAAGTTTT TGTTTCTGGA TCACCGTGCT 
CCTCCGATCA TTGGCTATAT GCCCTTCGAG GTTCTGGGGA CGTCCGGCTA TGATTATTAC 
CACTTTGATG ATCTGGACAG TATTGTGGCG TGCCATGAAG AGCTAAGGCA AACGGGTGAG 
GGCAAGTCTT GCTACTACCG ATTCCTTACG AAGGGCCAAC AGTGGATATG GTTGCAAACG 
GACTACTATG TGAGCTATCA CCAGTTCAAT TCCAAACCAG ACTACGTGGT CTGCACCCAT 
AAGGTTGTCA GCTATGCCGA GGTTCTGAAG GATAGTCGAA AGGAGGGCCA AAAATCGGGA 
AATAGCAACA GCATCACCAA CAATGGGAGC AGTAAGGTCA TAGCCTCCAC GGGAACTAGC 
AGCAAATCCG CATCTGCCAC CACAACACTG AGGGACTTCG AGCTGAGCAG CCAAAATCTG 
GATAGCACAT TGCTCGGAAA TAGTCTGGCC AGTTTGGGCA CTGAAACAGC AGCCACTTCA 
CCCGCCGTGG ACTCGTCGCC CATGTGGTCA GCGTCGGCTG TCCAACCGTC GGGATCATGC 
CAAATAAATC CCCTGAAGAC ATCGCGTCCC GCTTCGAGTT ATGGCAATAT CAGCTCCACA 
GGAATATCGC CGAAGGCCAA ACGAAAGTGC TACTTCTACA ACAACCGGGG GAATGACTCG 
GATTCAACGT CCATGTCGAC GGATTCGGTC ACCAGCCGGC AGTCCATGAT GACGCACGTC 
AGTTCGCAAA GCCAACGGCA GCGATCGCAT CACCGAGAAC ACCATCGAGA AAATCATCAC 
AATCAGTCAC ATCATCACAT GCAGCAACAG CAGCAGCATC AGAACCAGCA GCAGCAACAT 
CAGCAGCACC AGCAACTGCA ACAGCAGCTG CAACATACAG TGGGTACTCC GAAAATGGTG 
CCTCTCCTGC CGATTGCGTC CACACAAATA ATGGCTGGAA ATGCGTGTCA GTTTCCCCAA 
CCCGCCTATC CGCTTGCTAG TCCCCAACTG GTGGCACCGA CATTTCTTGA GCCGCCGCAA 
TATCTCACCG CCATTCCCAT GCAGCCAGTG ATCGCTCCGT TTCCGGTGGC GCCAGTGCTT 
TCCCCACTTC CGGTGCAGTC ACAAACGGAT ATGCTGCCCG ATACGGTGGT TATGACGCCA 
ACACAGAGTC AGTTGCAGGA TCAATTGCAG CGGAAGCACG ACGAACTGCA AAAACTGATC 
CTCCAGCAAC AGAATGAGCT GAGGATCGTA TCAGAGCAGC TCCTCCTATC CCGCTACACA 
TATCTGCAGC CCATGATGTC GATGGGATTC GCACCTGGCA ACATGACAGC AGCAGCGGTG 
GGTAATTTGG GAGCCAGTGG CCAACGTGGA CTGAATTTCA CCGGCAGCAA TGCCGTGCAG 
CCGCAATTCA ATCAGTATGG ATTTGCACTG AACTCGGAGC AGATGCTTAA CCAGCAGGAT 
CAGCAGATGA TGATGCAGCA GCAGCAGAAT CTGCACACGC AGCACCAGCA CAATCTCCAG 
CAGCAACATC AGAGCCACTC ACAGTTGCAG CAACATACCC AGCAGCAACA TCAGCAGCAG 
CAGCAACAAC AGCAACAACA GCAGCAACAG CAGCAGCAGC AACAGCAACA ACAGCAGCAG 
CAACAACAGC AGCAACAGCA GCAGCAACAG TTGCAGCTGC AACAACAAAA TGATATTTTA 
CTGCGTGAGG ATATCGATGA TATAGACGCC TTTCTCAATC TGTCCCCCCT GCACTCATTG 
GGCTCGCAAT CCACCATTAA TCCCTTCAAC TCCAGCAGCA ATAACAACAA TCAAAGCTAC 
AATGGAGGCA GTAATCTGAA TAATGGCAAC CAGAACAACA ACAATCGCAG TTCAAATCCT 
CCGCAAAATA ATAACGAGGA CTCGCTGTTG TCCTGCATGC AGATGGCCAC CGAATCGAGT 
CCTTCGATCA ACTTTCACAT GGGCATCAGC GATGATGGCT CCGAAACGCA GAGCGAGGAC 
AACAAGATGA TGCACACTTC CGGCAGCAAT CTGGTGCAGC AGCAACAGCA GCAGCAGCAA 
CAACAACAAA TCCTGCAGCA GCATCAACAG CAGTCAAATA GCTTCTTCAG TTCGAATCCC 
TTTCTCAACA GTCAGAATCA GAATCAAAAC CAATTGCCAA ACGATCTGGA AATATTGCCA 
TACCAGATGT CCCAAGAGCA ATCACAGAAT CTATTCAACT CGCCACACAC AGCCCCAGGC 
AGTAGTCAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clk ( NM_206299.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996021.2 Drosophila melanogaster clock, transcript variant H (Clk), mRNA.

Price & Availability↑

CloneID	OFa17834
Clone ID Related Accession (Same CDS sequence)	NM_206299.2 , NM_170628.2
Accession Version	NM_206299.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	clock, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_206299.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCATTT ATGACCTGGC CTATGAGATG GACCATGAGG CGCTGCTGAA TATCTTTATG 
AATCCCACAC CGGTAATCGA GCCCAGACAG ACGGACATAA GTTCCAGTAA CCAGATCACA 
TTCTACACTC ATTTGAGACG CGGCGGGATG GAGAAAGTGG ATGCCAATGC CTACGAGTTG 
GTCAAATTCG TGGGCTACTT TCGTAACGAT ACCAATACTT CGACGGGTTC CAGTTCGGAG 
GTTTCGAATG GCTCAAATGG ACAACCTGCG GTGCTGCCGC GCATCTTCCA GCAGAATCCC 
AATGCCGAGG TGGACAAAAA GCTGGTTTTC GTGGGCACTG GACGCGTACA GAATCCCCAA 
CTGATCAGGG AAATGAGCAT TATTGATCCC ACAAGTAATG AGTTCACTTC GAAACACAGC 
ATGGAGTGGA AGTTTTTGTT TCTGGATCAC CGTGCTCCTC CGATCATTGG CTATATGCCC 
TTCGAGGTTC TGGGGACGTC CGGCTATGAT TATTACCACT TTGATGATCT GGACAGTATT 
GTGGCGTGCC ATGAAGAGCT AAGGCAAACG GGTGAGGGCA AGTCTTGCTA CTACCGATTC 
CTTACGAAGG GCCAACAGTG GATATGGTTG CAAACGGACT ACTATGTGAG CTATCACCAG 
TTCAATTCCA AACCAGACTA CGTGGTCTGC ACCCATAAGG TTGTCAGCTA TGCCGAGGTT 
CTGAAGGATA GTCGAAAGGA GGGCCAAAAA TCGGGAAATA GCAACAGCAT CACCAACAAT 
GGGAGCAGTA AGGTCATAGC CTCCACGGGA ACTAGCAGCA AATCCGCATC TGCCACCACA 
ACACTGAGGG ACTTCGAGCT GAGCAGCCAA AATCTGGATA GCACATTGCT CGGAAATAGT 
CTGGCCAGTT TGGGCACTGA AACAGCAGCC ACTTCACCCG CCGTGGACTC GTCGCCCATG 
TGGTCAGCGT CGGCTGTCCA ACCGTCGGGA TCATGCCAAA TAAATCCCCT GAAGACATCG 
CGTCCCGCTT CGAGTTATGG CAATATCAGC TCCACAGGAA TATCGCCGAA GGCCAAACGA 
AAGTGCTACT TCTACAACAA CCGGGGGAAT GACTCGGATT CAACGTCCAT GTCGACGGAT 
TCGGTCACCA GCCGGCAGTC CATGATGACG CACGTCAGTT CGCAAAGCCA ACGGCAGCGA 
TCGCATCACC GAGAACACCA TCGAGAAAAT CATCACAATC AGTCACATCA TCACATGCAG 
CAACAGCAGC AGCATCAGAA CCAGCAGCAG CAACATCAGC AGCACCAGCA ACTGCAACAG 
CAGCTGCAAC ATACAGTGGG TACTCCGAAA ATGGTGCCTC TCCTGCCGAT TGCGTCCACA 
CAAATAATGG CTGGAAATGC GTGTCAGTTT CCCCAACCCG CCTATCCGCT TGCTAGTCCC 
CAACTGGTGG CACCGACATT TCTTGAGCCG CCGCAATATC TCACCGCCAT TCCCATGCAG 
CCAGTGATCG CTCCGTTTCC GGTGGCGCCA GTGCTTTCCC CACTTCCGGT GCAGTCACAA 
ACGGATATGC TGCCCGATAC GGTGGTTATG ACGCCAACAC AGAGTCAGTT GCAGGATCAA 
TTGCAGCGGA AGCACGACGA ACTGCAAAAA CTGATCCTCC AGCAACAGAA TGAGCTGAGG 
ATCGTATCAG AGCAGCTCCT CCTATCCCGC TACACATATC TGCAGCCCAT GATGTCGATG 
GGATTCGCAC CTGGCAACAT GACAGCAGCA GCGGTGGGTA ATTTGGGAGC CAGTGGCCAA 
CGTGGACTGA ATTTCACCGG CAGCAATGCC GTGCAGCCGC AATTCAATCA GTATGGATTT 
GCACTGAACT CGGAGCAGAT GCTTAACCAG CAGGATCAGC AGATGATGAT GCAGCAGCAG 
CAGAATCTGC ACACGCAGCA CCAGCACAAT CTCCAGCAGC AACATCAGAG CCACTCACAG 
TTGCAGCAAC ATACCCAGCA GCAACATCAG CAGCAGCAGC AACAACAGCA ACAACAGCAG 
CAACAGCAGC AGCAGCAACA GCAACAACAG CAGCAGCAAC AACAGCAGCA ACAGCAGCAG 
CAACAGTTGC AGCTGCAACA ACAAAATGAT ATTTTACTGC GTGAGGATAT CGATGATATA 
GACGCCTTTC TCAATCTGTC CCCCCTGCAC TCATTGGGCT CGCAATCCAC CATTAATCCC 
TTCAACTCCA GCAGCAATAA CAACAATCAA AGCTACAATG GAGGCAGTAA TCTGAATAAT 
GGCAACCAGA ACAACAACAA TCGCAGTTCA AATCCTCCGC AAAATAATAA CGAGGACTCG 
CTGTTGTCCT GCATGCAGAT GGCCACCGAA TCGAGTCCTT CGATCAACTT TCACATGGGC 
ATCAGCGATG ATGGCTCCGA AACGCAGAGC GAGGACAACA AGATGATGCA CACTTCCGGC 
AGCAATCTGG TGCAGCAGCA ACAGCAGCAG CAGCAACAAC AACAAATCCT GCAGCAGCAT 
CAACAGCAGT CAAATAGCTT CTTCAGTTCG AATCCCTTTC TCAACAGTCA GAATCAGAAT 
CAAAACCAAT TGCCAAACGA TCTGGAAATA TTGCCATACC AGATGTCCCA AGAGCAATCA 
CAGAATCTAT TCAACTCGCC ACACACAGCC CCAGGCAGTA GTCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996021.2
CDS	98..2785
Translation	MTIYDLAYEMDHEALLNIFMNPTPVIEPRQTDISSSNQITFYTHLRRGGMEKVDANAYELVKFVGYFRNDTNTSTGSSSEVSNGSNGQPAVLPRIFQQNPNAEVDKKLVFVGTGRVQNPQLIREMSIIDPTSNEFTSKHSMEWKFLFLDHRAPPIIGYMPFEVLGTSGYDYYHFDDLDSIVACHEELRQTGEGKSCYYRFLTKGQQWIWLQTDYYVSYHQFNSKPDYVVCTHKVVSYAEVLKDSRKEGQKSGNSNSITNNGSSKVIASTGTSSKSASATTTLRDFELSSQNLDSTLLGNSLASLGTETAATSPAVDSSPMWSASAVQPSGSCQINPLKTSRPASSYGNISSTGISPKAKRKCYFYNNRGNDSDSTSMSTDSVTSRQSMMTHVSSQSQRQRSHHREHHRENHHNQSHHHMQQQQQHQNQQQQHQQHQQLQQQLQHTVGTPKMVPLLPIASTQIMAGNACQFPQPAYPLASPQLVAPTFLEPPQYLTAIPMQPVIAPFPVAPVLSPLPVQSQTDMLPDTVVMTPTQSQLQDQLQRKHDELQKLILQQQNELRIVSEQLLLSRYTYLQPMMSMGFAPGNMTAAAVGNLGASGQRGLNFTGSNAVQPQFNQYGFALNSEQMLNQQDQQMMMQQQQNLHTQHQHNLQQQHQSHSQLQQHTQQQHQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQLQLQQQNDILLREDIDDIDAFLNLSPLHSLGSQSTINPFNSSSNNNNQSYNGGSNLNNGNQNNNNRSSNPPQNNNEDSLLSCMQMATESSPSINFHMGISDDGSETQSEDNKMMHTSGSNLVQQQQQQQQQQQILQQHQQQSNSFFSSNPFLNSQNQNQNQLPNDLEILPYQMSQEQSQNLFNSPHTAPGSSQ

Target ORF information:

RefSeq Version	NM_206299.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster clock, transcript variant H (Clk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206299.2

ORF Insert Sequence:

ATGACCATTT ATGACCTGGC CTATGAGATG GACCATGAGG CGCTGCTGAA TATCTTTATG 
AATCCCACAC CGGTAATCGA GCCCAGACAG ACGGACATAA GTTCCAGTAA CCAGATCACA 
TTCTACACTC ATTTGAGACG CGGCGGGATG GAGAAAGTGG ATGCCAATGC CTACGAGTTG 
GTCAAATTCG TGGGCTACTT TCGTAACGAT ACCAATACTT CGACGGGTTC CAGTTCGGAG 
GTTTCGAATG GCTCAAATGG ACAACCTGCG GTGCTGCCGC GCATCTTCCA GCAGAATCCC 
AATGCCGAGG TGGACAAAAA GCTGGTTTTC GTGGGCACTG GACGCGTACA GAATCCCCAA 
CTGATCAGGG AAATGAGCAT TATTGATCCC ACAAGTAATG AGTTCACTTC GAAACACAGC 
ATGGAGTGGA AGTTTTTGTT TCTGGATCAC CGTGCTCCTC CGATCATTGG CTATATGCCC 
TTCGAGGTTC TGGGGACGTC CGGCTATGAT TATTACCACT TTGATGATCT GGACAGTATT 
GTGGCGTGCC ATGAAGAGCT AAGGCAAACG GGTGAGGGCA AGTCTTGCTA CTACCGATTC 
CTTACGAAGG GCCAACAGTG GATATGGTTG CAAACGGACT ACTATGTGAG CTATCACCAG 
TTCAATTCCA AACCAGACTA CGTGGTCTGC ACCCATAAGG TTGTCAGCTA TGCCGAGGTT 
CTGAAGGATA GTCGAAAGGA GGGCCAAAAA TCGGGAAATA GCAACAGCAT CACCAACAAT 
GGGAGCAGTA AGGTCATAGC CTCCACGGGA ACTAGCAGCA AATCCGCATC TGCCACCACA 
ACACTGAGGG ACTTCGAGCT GAGCAGCCAA AATCTGGATA GCACATTGCT CGGAAATAGT 
CTGGCCAGTT TGGGCACTGA AACAGCAGCC ACTTCACCCG CCGTGGACTC GTCGCCCATG 
TGGTCAGCGT CGGCTGTCCA ACCGTCGGGA TCATGCCAAA TAAATCCCCT GAAGACATCG 
CGTCCCGCTT CGAGTTATGG CAATATCAGC TCCACAGGAA TATCGCCGAA GGCCAAACGA 
AAGTGCTACT TCTACAACAA CCGGGGGAAT GACTCGGATT CAACGTCCAT GTCGACGGAT 
TCGGTCACCA GCCGGCAGTC CATGATGACG CACGTCAGTT CGCAAAGCCA ACGGCAGCGA 
TCGCATCACC GAGAACACCA TCGAGAAAAT CATCACAATC AGTCACATCA TCACATGCAG 
CAACAGCAGC AGCATCAGAA CCAGCAGCAG CAACATCAGC AGCACCAGCA ACTGCAACAG 
CAGCTGCAAC ATACAGTGGG TACTCCGAAA ATGGTGCCTC TCCTGCCGAT TGCGTCCACA 
CAAATAATGG CTGGAAATGC GTGTCAGTTT CCCCAACCCG CCTATCCGCT TGCTAGTCCC 
CAACTGGTGG CACCGACATT TCTTGAGCCG CCGCAATATC TCACCGCCAT TCCCATGCAG 
CCAGTGATCG CTCCGTTTCC GGTGGCGCCA GTGCTTTCCC CACTTCCGGT GCAGTCACAA 
ACGGATATGC TGCCCGATAC GGTGGTTATG ACGCCAACAC AGAGTCAGTT GCAGGATCAA 
TTGCAGCGGA AGCACGACGA ACTGCAAAAA CTGATCCTCC AGCAACAGAA TGAGCTGAGG 
ATCGTATCAG AGCAGCTCCT CCTATCCCGC TACACATATC TGCAGCCCAT GATGTCGATG 
GGATTCGCAC CTGGCAACAT GACAGCAGCA GCGGTGGGTA ATTTGGGAGC CAGTGGCCAA 
CGTGGACTGA ATTTCACCGG CAGCAATGCC GTGCAGCCGC AATTCAATCA GTATGGATTT 
GCACTGAACT CGGAGCAGAT GCTTAACCAG CAGGATCAGC AGATGATGAT GCAGCAGCAG 
CAGAATCTGC ACACGCAGCA CCAGCACAAT CTCCAGCAGC AACATCAGAG CCACTCACAG 
TTGCAGCAAC ATACCCAGCA GCAACATCAG CAGCAGCAGC AACAACAGCA ACAACAGCAG 
CAACAGCAGC AGCAGCAACA GCAACAACAG CAGCAGCAAC AACAGCAGCA ACAGCAGCAG 
CAACAGTTGC AGCTGCAACA ACAAAATGAT ATTTTACTGC GTGAGGATAT CGATGATATA 
GACGCCTTTC TCAATCTGTC CCCCCTGCAC TCATTGGGCT CGCAATCCAC CATTAATCCC 
TTCAACTCCA GCAGCAATAA CAACAATCAA AGCTACAATG GAGGCAGTAA TCTGAATAAT 
GGCAACCAGA ACAACAACAA TCGCAGTTCA AATCCTCCGC AAAATAATAA CGAGGACTCG 
CTGTTGTCCT GCATGCAGAT GGCCACCGAA TCGAGTCCTT CGATCAACTT TCACATGGGC 
ATCAGCGATG ATGGCTCCGA AACGCAGAGC GAGGACAACA AGATGATGCA CACTTCCGGC 
AGCAATCTGG TGCAGCAGCA ACAGCAGCAG CAGCAACAAC AACAAATCCT GCAGCAGCAT 
CAACAGCAGT CAAATAGCTT CTTCAGTTCG AATCCCTTTC TCAACAGTCA GAATCAGAAT 
CAAAACCAAT TGCCAAACGA TCTGGAAATA TTGCCATACC AGATGTCCCA AGAGCAATCA 
CAGAATCTAT TCAACTCGCC ACACACAGCC CCAGGCAGTA GTCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clk ( NM_170628.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729294.2 Drosophila melanogaster clock, transcript variant G (Clk), mRNA.

Price & Availability↑

CloneID	OFa17834
Clone ID Related Accession (Same CDS sequence)	NM_206299.2 , NM_170628.2
Accession Version	NM_170628.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	clock, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_170628.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCATTT ATGACCTGGC CTATGAGATG GACCATGAGG CGCTGCTGAA TATCTTTATG 
AATCCCACAC CGGTAATCGA GCCCAGACAG ACGGACATAA GTTCCAGTAA CCAGATCACA 
TTCTACACTC ATTTGAGACG CGGCGGGATG GAGAAAGTGG ATGCCAATGC CTACGAGTTG 
GTCAAATTCG TGGGCTACTT TCGTAACGAT ACCAATACTT CGACGGGTTC CAGTTCGGAG 
GTTTCGAATG GCTCAAATGG ACAACCTGCG GTGCTGCCGC GCATCTTCCA GCAGAATCCC 
AATGCCGAGG TGGACAAAAA GCTGGTTTTC GTGGGCACTG GACGCGTACA GAATCCCCAA 
CTGATCAGGG AAATGAGCAT TATTGATCCC ACAAGTAATG AGTTCACTTC GAAACACAGC 
ATGGAGTGGA AGTTTTTGTT TCTGGATCAC CGTGCTCCTC CGATCATTGG CTATATGCCC 
TTCGAGGTTC TGGGGACGTC CGGCTATGAT TATTACCACT TTGATGATCT GGACAGTATT 
GTGGCGTGCC ATGAAGAGCT AAGGCAAACG GGTGAGGGCA AGTCTTGCTA CTACCGATTC 
CTTACGAAGG GCCAACAGTG GATATGGTTG CAAACGGACT ACTATGTGAG CTATCACCAG 
TTCAATTCCA AACCAGACTA CGTGGTCTGC ACCCATAAGG TTGTCAGCTA TGCCGAGGTT 
CTGAAGGATA GTCGAAAGGA GGGCCAAAAA TCGGGAAATA GCAACAGCAT CACCAACAAT 
GGGAGCAGTA AGGTCATAGC CTCCACGGGA ACTAGCAGCA AATCCGCATC TGCCACCACA 
ACACTGAGGG ACTTCGAGCT GAGCAGCCAA AATCTGGATA GCACATTGCT CGGAAATAGT 
CTGGCCAGTT TGGGCACTGA AACAGCAGCC ACTTCACCCG CCGTGGACTC GTCGCCCATG 
TGGTCAGCGT CGGCTGTCCA ACCGTCGGGA TCATGCCAAA TAAATCCCCT GAAGACATCG 
CGTCCCGCTT CGAGTTATGG CAATATCAGC TCCACAGGAA TATCGCCGAA GGCCAAACGA 
AAGTGCTACT TCTACAACAA CCGGGGGAAT GACTCGGATT CAACGTCCAT GTCGACGGAT 
TCGGTCACCA GCCGGCAGTC CATGATGACG CACGTCAGTT CGCAAAGCCA ACGGCAGCGA 
TCGCATCACC GAGAACACCA TCGAGAAAAT CATCACAATC AGTCACATCA TCACATGCAG 
CAACAGCAGC AGCATCAGAA CCAGCAGCAG CAACATCAGC AGCACCAGCA ACTGCAACAG 
CAGCTGCAAC ATACAGTGGG TACTCCGAAA ATGGTGCCTC TCCTGCCGAT TGCGTCCACA 
CAAATAATGG CTGGAAATGC GTGTCAGTTT CCCCAACCCG CCTATCCGCT TGCTAGTCCC 
CAACTGGTGG CACCGACATT TCTTGAGCCG CCGCAATATC TCACCGCCAT TCCCATGCAG 
CCAGTGATCG CTCCGTTTCC GGTGGCGCCA GTGCTTTCCC CACTTCCGGT GCAGTCACAA 
ACGGATATGC TGCCCGATAC GGTGGTTATG ACGCCAACAC AGAGTCAGTT GCAGGATCAA 
TTGCAGCGGA AGCACGACGA ACTGCAAAAA CTGATCCTCC AGCAACAGAA TGAGCTGAGG 
ATCGTATCAG AGCAGCTCCT CCTATCCCGC TACACATATC TGCAGCCCAT GATGTCGATG 
GGATTCGCAC CTGGCAACAT GACAGCAGCA GCGGTGGGTA ATTTGGGAGC CAGTGGCCAA 
CGTGGACTGA ATTTCACCGG CAGCAATGCC GTGCAGCCGC AATTCAATCA GTATGGATTT 
GCACTGAACT CGGAGCAGAT GCTTAACCAG CAGGATCAGC AGATGATGAT GCAGCAGCAG 
CAGAATCTGC ACACGCAGCA CCAGCACAAT CTCCAGCAGC AACATCAGAG CCACTCACAG 
TTGCAGCAAC ATACCCAGCA GCAACATCAG CAGCAGCAGC AACAACAGCA ACAACAGCAG 
CAACAGCAGC AGCAGCAACA GCAACAACAG CAGCAGCAAC AACAGCAGCA ACAGCAGCAG 
CAACAGTTGC AGCTGCAACA ACAAAATGAT ATTTTACTGC GTGAGGATAT CGATGATATA 
GACGCCTTTC TCAATCTGTC CCCCCTGCAC TCATTGGGCT CGCAATCCAC CATTAATCCC 
TTCAACTCCA GCAGCAATAA CAACAATCAA AGCTACAATG GAGGCAGTAA TCTGAATAAT 
GGCAACCAGA ACAACAACAA TCGCAGTTCA AATCCTCCGC AAAATAATAA CGAGGACTCG 
CTGTTGTCCT GCATGCAGAT GGCCACCGAA TCGAGTCCTT CGATCAACTT TCACATGGGC 
ATCAGCGATG ATGGCTCCGA AACGCAGAGC GAGGACAACA AGATGATGCA CACTTCCGGC 
AGCAATCTGG TGCAGCAGCA ACAGCAGCAG CAGCAACAAC AACAAATCCT GCAGCAGCAT 
CAACAGCAGT CAAATAGCTT CTTCAGTTCG AATCCCTTTC TCAACAGTCA GAATCAGAAT 
CAAAACCAAT TGCCAAACGA TCTGGAAATA TTGCCATACC AGATGTCCCA AGAGCAATCA 
CAGAATCTAT TCAACTCGCC ACACACAGCC CCAGGCAGTA GTCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729294.2
CDS	98..2785
Translation	MTIYDLAYEMDHEALLNIFMNPTPVIEPRQTDISSSNQITFYTHLRRGGMEKVDANAYELVKFVGYFRNDTNTSTGSSSEVSNGSNGQPAVLPRIFQQNPNAEVDKKLVFVGTGRVQNPQLIREMSIIDPTSNEFTSKHSMEWKFLFLDHRAPPIIGYMPFEVLGTSGYDYYHFDDLDSIVACHEELRQTGEGKSCYYRFLTKGQQWIWLQTDYYVSYHQFNSKPDYVVCTHKVVSYAEVLKDSRKEGQKSGNSNSITNNGSSKVIASTGTSSKSASATTTLRDFELSSQNLDSTLLGNSLASLGTETAATSPAVDSSPMWSASAVQPSGSCQINPLKTSRPASSYGNISSTGISPKAKRKCYFYNNRGNDSDSTSMSTDSVTSRQSMMTHVSSQSQRQRSHHREHHRENHHNQSHHHMQQQQQHQNQQQQHQQHQQLQQQLQHTVGTPKMVPLLPIASTQIMAGNACQFPQPAYPLASPQLVAPTFLEPPQYLTAIPMQPVIAPFPVAPVLSPLPVQSQTDMLPDTVVMTPTQSQLQDQLQRKHDELQKLILQQQNELRIVSEQLLLSRYTYLQPMMSMGFAPGNMTAAAVGNLGASGQRGLNFTGSNAVQPQFNQYGFALNSEQMLNQQDQQMMMQQQQNLHTQHQHNLQQQHQSHSQLQQHTQQQHQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQLQLQQQNDILLREDIDDIDAFLNLSPLHSLGSQSTINPFNSSSNNNNQSYNGGSNLNNGNQNNNNRSSNPPQNNNEDSLLSCMQMATESSPSINFHMGISDDGSETQSEDNKMMHTSGSNLVQQQQQQQQQQQILQQHQQQSNSFFSSNPFLNSQNQNQNQLPNDLEILPYQMSQEQSQNLFNSPHTAPGSSQ

Target ORF information:

RefSeq Version	NM_170628.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster clock, transcript variant G (Clk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170628.2

ORF Insert Sequence:

ATGACCATTT ATGACCTGGC CTATGAGATG GACCATGAGG CGCTGCTGAA TATCTTTATG 
AATCCCACAC CGGTAATCGA GCCCAGACAG ACGGACATAA GTTCCAGTAA CCAGATCACA 
TTCTACACTC ATTTGAGACG CGGCGGGATG GAGAAAGTGG ATGCCAATGC CTACGAGTTG 
GTCAAATTCG TGGGCTACTT TCGTAACGAT ACCAATACTT CGACGGGTTC CAGTTCGGAG 
GTTTCGAATG GCTCAAATGG ACAACCTGCG GTGCTGCCGC GCATCTTCCA GCAGAATCCC 
AATGCCGAGG TGGACAAAAA GCTGGTTTTC GTGGGCACTG GACGCGTACA GAATCCCCAA 
CTGATCAGGG AAATGAGCAT TATTGATCCC ACAAGTAATG AGTTCACTTC GAAACACAGC 
ATGGAGTGGA AGTTTTTGTT TCTGGATCAC CGTGCTCCTC CGATCATTGG CTATATGCCC 
TTCGAGGTTC TGGGGACGTC CGGCTATGAT TATTACCACT TTGATGATCT GGACAGTATT 
GTGGCGTGCC ATGAAGAGCT AAGGCAAACG GGTGAGGGCA AGTCTTGCTA CTACCGATTC 
CTTACGAAGG GCCAACAGTG GATATGGTTG CAAACGGACT ACTATGTGAG CTATCACCAG 
TTCAATTCCA AACCAGACTA CGTGGTCTGC ACCCATAAGG TTGTCAGCTA TGCCGAGGTT 
CTGAAGGATA GTCGAAAGGA GGGCCAAAAA TCGGGAAATA GCAACAGCAT CACCAACAAT 
GGGAGCAGTA AGGTCATAGC CTCCACGGGA ACTAGCAGCA AATCCGCATC TGCCACCACA 
ACACTGAGGG ACTTCGAGCT GAGCAGCCAA AATCTGGATA GCACATTGCT CGGAAATAGT 
CTGGCCAGTT TGGGCACTGA AACAGCAGCC ACTTCACCCG CCGTGGACTC GTCGCCCATG 
TGGTCAGCGT CGGCTGTCCA ACCGTCGGGA TCATGCCAAA TAAATCCCCT GAAGACATCG 
CGTCCCGCTT CGAGTTATGG CAATATCAGC TCCACAGGAA TATCGCCGAA GGCCAAACGA 
AAGTGCTACT TCTACAACAA CCGGGGGAAT GACTCGGATT CAACGTCCAT GTCGACGGAT 
TCGGTCACCA GCCGGCAGTC CATGATGACG CACGTCAGTT CGCAAAGCCA ACGGCAGCGA 
TCGCATCACC GAGAACACCA TCGAGAAAAT CATCACAATC AGTCACATCA TCACATGCAG 
CAACAGCAGC AGCATCAGAA CCAGCAGCAG CAACATCAGC AGCACCAGCA ACTGCAACAG 
CAGCTGCAAC ATACAGTGGG TACTCCGAAA ATGGTGCCTC TCCTGCCGAT TGCGTCCACA 
CAAATAATGG CTGGAAATGC GTGTCAGTTT CCCCAACCCG CCTATCCGCT TGCTAGTCCC 
CAACTGGTGG CACCGACATT TCTTGAGCCG CCGCAATATC TCACCGCCAT TCCCATGCAG 
CCAGTGATCG CTCCGTTTCC GGTGGCGCCA GTGCTTTCCC CACTTCCGGT GCAGTCACAA 
ACGGATATGC TGCCCGATAC GGTGGTTATG ACGCCAACAC AGAGTCAGTT GCAGGATCAA 
TTGCAGCGGA AGCACGACGA ACTGCAAAAA CTGATCCTCC AGCAACAGAA TGAGCTGAGG 
ATCGTATCAG AGCAGCTCCT CCTATCCCGC TACACATATC TGCAGCCCAT GATGTCGATG 
GGATTCGCAC CTGGCAACAT GACAGCAGCA GCGGTGGGTA ATTTGGGAGC CAGTGGCCAA 
CGTGGACTGA ATTTCACCGG CAGCAATGCC GTGCAGCCGC AATTCAATCA GTATGGATTT 
GCACTGAACT CGGAGCAGAT GCTTAACCAG CAGGATCAGC AGATGATGAT GCAGCAGCAG 
CAGAATCTGC ACACGCAGCA CCAGCACAAT CTCCAGCAGC AACATCAGAG CCACTCACAG 
TTGCAGCAAC ATACCCAGCA GCAACATCAG CAGCAGCAGC AACAACAGCA ACAACAGCAG 
CAACAGCAGC AGCAGCAACA GCAACAACAG CAGCAGCAAC AACAGCAGCA ACAGCAGCAG 
CAACAGTTGC AGCTGCAACA ACAAAATGAT ATTTTACTGC GTGAGGATAT CGATGATATA 
GACGCCTTTC TCAATCTGTC CCCCCTGCAC TCATTGGGCT CGCAATCCAC CATTAATCCC 
TTCAACTCCA GCAGCAATAA CAACAATCAA AGCTACAATG GAGGCAGTAA TCTGAATAAT 
GGCAACCAGA ACAACAACAA TCGCAGTTCA AATCCTCCGC AAAATAATAA CGAGGACTCG 
CTGTTGTCCT GCATGCAGAT GGCCACCGAA TCGAGTCCTT CGATCAACTT TCACATGGGC 
ATCAGCGATG ATGGCTCCGA AACGCAGAGC GAGGACAACA AGATGATGCA CACTTCCGGC 
AGCAATCTGG TGCAGCAGCA ACAGCAGCAG CAGCAACAAC AACAAATCCT GCAGCAGCAT 
CAACAGCAGT CAAATAGCTT CTTCAGTTCG AATCCCTTTC TCAACAGTCA GAATCAGAAT 
CAAAACCAAT TGCCAAACGA TCTGGAAATA TTGCCATACC AGATGTCCCA AGAGCAATCA 
CAGAATCTAT TCAACTCGCC ACACACAGCC CCAGGCAGTA GTCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clk ( NM_001014574.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014574.1 Drosophila melanogaster clock, transcript variant F (Clk), mRNA.

Price & Availability↑

CloneID	OFa17835
Clone ID Related Accession (Same CDS sequence)	NM_001014574.2
Accession Version	NM_001014574.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	clock, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001014574.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACCG ATCGCTCCAA GGTTTTCGAA ATACAACAGG ACTGGAAGCC TGCGTTCCTC 
AGCAATGACG AATACACTCA CCTCATGCTG GAGTCTCTCG ATGGTTTTAT GATGGTCTTC 
AGCAGCATGG GCTCCATCTT TTACGCCTCG GAAAGTATTA CCTCCCAGCT GGGCTATCTG 
CCGCAAGATT TGTACAACAT GACCATTTAT GACCTGGCCT ATGAGATGGA CCATGAGGCG 
CTGCTGAATA TCTTTATGAA TCCCACACCG GTAATCGAGC CCAGACAGAC GGACATAAGT 
TCCAGTAACC AGATCACATT CTACACTCAT TTGAGACGCG GCGGGATGGA GAAAGTGGAT 
GCCAATGCCT ACGAGTTGGT CAAATTCGTG GGCTACTTTC GTAACGATAC CAATACTTCG 
ACGGGTTCCA GTTCGGAGGT TTCGAATGGC TCAAATGGAC AACCTGCGGT GCTGCCGCGC 
ATCTTCCAGC AGAATCCCAA TGCCGAGGTG GACAAAAAGC TGGTTTTCGT GGGCACTGGA 
CGCGTACAGA ATCCCCAACT GATCAGGGAA ATGAGCATTA TTGATCCCAC AAGTAATGAG 
TTCACTTCGA AACACAGCAT GGAGTGGAAG TTTTTGTTTC TGGATCACCG TGCTCCTCCG 
ATCATTGGCT ATATGCCCTT CGAGGTTCTG GGGACGTCCG GCTATGATTA TTACCACTTT 
GATGATCTGG ACAGTATTGT GGCGTGCCAT GAAGAGCTAA GGCAAACGGG TGAGGGCAAG 
TCTTGCTACT ACCGATTCCT TACGAAGGGC CAACAGTGGA TATGGTTGCA AACGGACTAC 
TATGTGAGCT ATCACCAGTT CAATTCCAAA CCAGACTACG TGGTCTGCAC CCATAAGGTT 
GTCAGCTATG CCGAGGTTCT GAAGGATAGT CGAAAGGAGG GCCAAAAATC GGGAAATAGC 
AACAGCATCA CCAACAATGG GAGCAGTAAG GTCATAGCCT CCACGGGAAC TAGCAGCAAA 
TCCGCATCTG CCACCACAAC ACTGAGGGAC TTCGAGCTGA GCAGCCAAAA TCTGGATAGC 
ACATTGCTCG GAAATAGTCT GGCCAGTTTG GGCACTGAAA CAGCAGCCAC TTCACCCGCC 
GTGGACTCGT CGCCCATGTG GTCAGCGTCG GCTGTCCAAC CGTCGGGATC ATGCCAAATA 
AATCCCCTGA AGACATCGCG TCCCGCTTCG AGTTATGGCA ATATCAGCTC CACAGGAATA 
TCGCCGAAGG CCAAACGAAA GTGCTACTTC TACAACAACC GGGGGAATGA CTCGGATTCA 
ACGTCCATGT CGACGGATTC GGTCACCAGC CGGCAGTCCA TGATGACGCA CGTCAGTTCG 
CAAAGCCAAC GGCAGCGATC GCATCACCGA GAACACCATC GAGAAAATCA TCACAATCAG 
TCACATCATC ACATGCAGCA ACAGCAGCAG CATCAGAACC AGCAGCAGCA ACATCAGCAG 
CACCAGCAAC TGCAACAGCA GCTGCAACAT ACAGTGGGTA CTCCGAAAAT GGTGCCTCTC 
CTGCCGATTG CGTCCACACA AATAATGGCT GGAAATGCGT GTCAGTTTCC CCAACCCGCC 
TATCCGCTTG CTAGTCCCCA ACTGGTGGCA CCGACATTTC TTGAGCCGCC GCAATATCTC 
ACCGCCATTC CCATGCAGCC AGTGATCGCT CCGTTTCCGG TGGCGCCAGT GCTTTCCCCA 
CTTCCGGTGC AGTCACAAAC GGATATGCTG CCCGATACGG TGGTTATGAC GCCAACACAG 
AGTCAGTTGC AGGATCAATT GCAGCGGAAG CACGACGAAC TGCAAAAACT GATCCTCCAG 
CAACAGAATG AGCTGAGGAT CGTATCAGAG CAGCTCCTCC TATCCCGCTA CACATATCTG 
CAGCCCATGA TGTCGATGGG ATTCGCACCT GGCAACATGA CAGCAGCAGC GGTGGGTAAT 
TTGGGAGCCA GTGGCCAACG TGGACTGAAT TTCACCGGCA GCAATGCCGT GCAGCCGCAA 
TTCAATCAGT ATGGATTTGC ACTGAACTCG GAGCAGATGC TTAACCAGCA GGATCAGCAG 
ATGATGATGC AGCAGCAGCA GAATCTGCAC ACGCAGCACC AGCACAATCT CCAGCAGCAA 
CATCAGAGCC ACTCACAGTT GCAGCAACAT ACCCAGCAGC AACATCAGCA GCAGCAGCAA 
CAACAGCAAC AACAGCAGCA ACAGCAGCAG CAGCAACAGC AACAACAGCA GCAGCAACAA 
CAGCAGCAAC AGCAGCAGCA ACAGTTGCAG CTGCAACAAC AAAATGATAT TTTACTGCGT 
GAGGATATCG ATGATATAGA CGCCTTTCTC AATCTGTCCC CCCTGCACTC ATTGGGCTCG 
CAATCCACCA TTAATCCCTT CAACTCCAGC AGCAATAACA ACAATCAAAG CTACAATGGA 
GGCAGTAATC TGAATAATGG CAACCAGAAC AACAACAATC GCAGTTCAAA TCCTCCGCAA 
AATAATAACG AGGACTCGCT GTTGTCCTGC ATGCAGATGG CCACCGAATC GAGTCCTTCG 
ATCAACTTTC ACATGGGCAT CAGCGATGAT GGCTCCGAAA CGCAGAGCGA GGACAACAAG 
ATGATGCACA CTTCCGGCAG CAATCTGGTG CAGCAGCAAC AGCAGCAGCA GCAACAACAA 
CAAATCCTGC AGCAGCATCA ACAGCAGTCA AATAGCTTCT TCAGTTCGAA TCCCTTTCTC 
AACAGTCAGA ATCAGAATCA AAACCAATTG CCAAACGATC TGGAAATATT GCCATACCAG 
ATGTCCCAAG AGCAATCACA GAATCTATTC AACTCGCCAC ACACAGCCCC AGGCAGTAGT 
CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014574.1
CDS	567..3452
Translation	MATDRSKVFEIQQDWKPAFLSNDEYTHLMLESLDGFMMVFSSMGSIFYASESITSQLGYLPQDLYNMTIYDLAYEMDHEALLNIFMNPTPVIEPRQTDISSSNQITFYTHLRRGGMEKVDANAYELVKFVGYFRNDTNTSTGSSSEVSNGSNGQPAVLPRIFQQNPNAEVDKKLVFVGTGRVQNPQLIREMSIIDPTSNEFTSKHSMEWKFLFLDHRAPPIIGYMPFEVLGTSGYDYYHFDDLDSIVACHEELRQTGEGKSCYYRFLTKGQQWIWLQTDYYVSYHQFNSKPDYVVCTHKVVSYAEVLKDSRKEGQKSGNSNSITNNGSSKVIASTGTSSKSASATTTLRDFELSSQNLDSTLLGNSLASLGTETAATSPAVDSSPMWSASAVQPSGSCQINPLKTSRPASSYGNISSTGISPKAKRKCYFYNNRGNDSDSTSMSTDSVTSRQSMMTHVSSQSQRQRSHHREHHRENHHNQSHHHMQQQQQHQNQQQQHQQHQQLQQQLQHTVGTPKMVPLLPIASTQIMAGNACQFPQPAYPLASPQLVAPTFLEPPQYLTAIPMQPVIAPFPVAPVLSPLPVQSQTDMLPDTVVMTPTQSQLQDQLQRKHDELQKLILQQQNELRIVSEQLLLSRYTYLQPMMSMGFAPGNMTAAAVGNLGASGQRGLNFTGSNAVQPQFNQYGFALNSEQMLNQQDQQMMMQQQQNLHTQHQHNLQQQHQSHSQLQQHTQQQHQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQLQLQQQNDILLREDIDDIDAFLNLSPLHSLGSQSTINPFNSSSNNNNQSYNGGSNLNNGNQNNNNRSSNPPQNNNEDSLLSCMQMATESSPSINFHMGISDDGSETQSEDNKMMHTSGSNLVQQQQQQQQQQQILQQHQQQSNSFFSSNPFLNSQNQNQNQLPNDLEILPYQMSQEQSQNLFNSPHTAPGSSQ

Target ORF information:

RefSeq Version	NM_001014574.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster clock, transcript variant F (Clk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014574.2

ORF Insert Sequence:

ATGGCCACCG ATCGCTCCAA GGTTTTCGAA ATACAACAGG ACTGGAAGCC TGCGTTCCTC 
AGCAATGACG AATACACTCA CCTCATGCTG GAGTCTCTCG ATGGTTTTAT GATGGTCTTC 
AGCAGCATGG GCTCCATCTT TTACGCCTCG GAAAGTATTA CCTCCCAGCT GGGCTATCTG 
CCGCAAGATT TGTACAACAT GACCATTTAT GACCTGGCCT ATGAGATGGA CCATGAGGCG 
CTGCTGAATA TCTTTATGAA TCCCACACCG GTAATCGAGC CCAGACAGAC GGACATAAGT 
TCCAGTAACC AGATCACATT CTACACTCAT TTGAGACGCG GCGGGATGGA GAAAGTGGAT 
GCCAATGCCT ACGAGTTGGT CAAATTCGTG GGCTACTTTC GTAACGATAC CAATACTTCG 
ACGGGTTCCA GTTCGGAGGT TTCGAATGGC TCAAATGGAC AACCTGCGGT GCTGCCGCGC 
ATCTTCCAGC AGAATCCCAA TGCCGAGGTG GACAAAAAGC TGGTTTTCGT GGGCACTGGA 
CGCGTACAGA ATCCCCAACT GATCAGGGAA ATGAGCATTA TTGATCCCAC AAGTAATGAG 
TTCACTTCGA AACACAGCAT GGAGTGGAAG TTTTTGTTTC TGGATCACCG TGCTCCTCCG 
ATCATTGGCT ATATGCCCTT CGAGGTTCTG GGGACGTCCG GCTATGATTA TTACCACTTT 
GATGATCTGG ACAGTATTGT GGCGTGCCAT GAAGAGCTAA GGCAAACGGG TGAGGGCAAG 
TCTTGCTACT ACCGATTCCT TACGAAGGGC CAACAGTGGA TATGGTTGCA AACGGACTAC 
TATGTGAGCT ATCACCAGTT CAATTCCAAA CCAGACTACG TGGTCTGCAC CCATAAGGTT 
GTCAGCTATG CCGAGGTTCT GAAGGATAGT CGAAAGGAGG GCCAAAAATC GGGAAATAGC 
AACAGCATCA CCAACAATGG GAGCAGTAAG GTCATAGCCT CCACGGGAAC TAGCAGCAAA 
TCCGCATCTG CCACCACAAC ACTGAGGGAC TTCGAGCTGA GCAGCCAAAA TCTGGATAGC 
ACATTGCTCG GAAATAGTCT GGCCAGTTTG GGCACTGAAA CAGCAGCCAC TTCACCCGCC 
GTGGACTCGT CGCCCATGTG GTCAGCGTCG GCTGTCCAAC CGTCGGGATC ATGCCAAATA 
AATCCCCTGA AGACATCGCG TCCCGCTTCG AGTTATGGCA ATATCAGCTC CACAGGAATA 
TCGCCGAAGG CCAAACGAAA GTGCTACTTC TACAACAACC GGGGGAATGA CTCGGATTCA 
ACGTCCATGT CGACGGATTC GGTCACCAGC CGGCAGTCCA TGATGACGCA CGTCAGTTCG 
CAAAGCCAAC GGCAGCGATC GCATCACCGA GAACACCATC GAGAAAATCA TCACAATCAG 
TCACATCATC ACATGCAGCA ACAGCAGCAG CATCAGAACC AGCAGCAGCA ACATCAGCAG 
CACCAGCAAC TGCAACAGCA GCTGCAACAT ACAGTGGGTA CTCCGAAAAT GGTGCCTCTC 
CTGCCGATTG CGTCCACACA AATAATGGCT GGAAATGCGT GTCAGTTTCC CCAACCCGCC 
TATCCGCTTG CTAGTCCCCA ACTGGTGGCA CCGACATTTC TTGAGCCGCC GCAATATCTC 
ACCGCCATTC CCATGCAGCC AGTGATCGCT CCGTTTCCGG TGGCGCCAGT GCTTTCCCCA 
CTTCCGGTGC AGTCACAAAC GGATATGCTG CCCGATACGG TGGTTATGAC GCCAACACAG 
AGTCAGTTGC AGGATCAATT GCAGCGGAAG CACGACGAAC TGCAAAAACT GATCCTCCAG 
CAACAGAATG AGCTGAGGAT CGTATCAGAG CAGCTCCTCC TATCCCGCTA CACATATCTG 
CAGCCCATGA TGTCGATGGG ATTCGCACCT GGCAACATGA CAGCAGCAGC GGTGGGTAAT 
TTGGGAGCCA GTGGCCAACG TGGACTGAAT TTCACCGGCA GCAATGCCGT GCAGCCGCAA 
TTCAATCAGT ATGGATTTGC ACTGAACTCG GAGCAGATGC TTAACCAGCA GGATCAGCAG 
ATGATGATGC AGCAGCAGCA GAATCTGCAC ACGCAGCACC AGCACAATCT CCAGCAGCAA 
CATCAGAGCC ACTCACAGTT GCAGCAACAT ACCCAGCAGC AACATCAGCA GCAGCAGCAA 
CAACAGCAAC AACAGCAGCA ACAGCAGCAG CAGCAACAGC AACAACAGCA GCAGCAACAA 
CAGCAGCAAC AGCAGCAGCA ACAGTTGCAG CTGCAACAAC AAAATGATAT TTTACTGCGT 
GAGGATATCG ATGATATAGA CGCCTTTCTC AATCTGTCCC CCCTGCACTC ATTGGGCTCG 
CAATCCACCA TTAATCCCTT CAACTCCAGC AGCAATAACA ACAATCAAAG CTACAATGGA 
GGCAGTAATC TGAATAATGG CAACCAGAAC AACAACAATC GCAGTTCAAA TCCTCCGCAA 
AATAATAACG AGGACTCGCT GTTGTCCTGC ATGCAGATGG CCACCGAATC GAGTCCTTCG 
ATCAACTTTC ACATGGGCAT CAGCGATGAT GGCTCCGAAA CGCAGAGCGA GGACAACAAG 
ATGATGCACA CTTCCGGCAG CAATCTGGTG CAGCAGCAAC AGCAGCAGCA GCAACAACAA 
CAAATCCTGC AGCAGCATCA ACAGCAGTCA AATAGCTTCT TCAGTTCGAA TCCCTTTCTC 
AACAGTCAGA ATCAGAATCA AAACCAATTG CCAAACGATC TGGAAATATT GCCATACCAG 
ATGTCCCAAG AGCAATCACA GAATCTATTC AACTCGCCAC ACACAGCCCC AGGCAGTAGT 
CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clk ( NM_001014576.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014576.1 Drosophila melanogaster clock, transcript variant D (Clk), mRNA.

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CloneID	OFa17836
Clone ID Related Accession (Same CDS sequence)	NM_001014576.2
Accession Version	NM_001014576.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	clock, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001014576.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGACG AGAGCGACGA CAAGGATGAT ACAAAAAGCT TCCTTTGCAG GAAATCGCGT 
AATCTCAGCG AGAAGAAGCG ACGAGATCAG TTCAACTCGC TGGTCAACGA TCTCAGCGCC 
TTGATATCCA CATCCAGCAG AAAGATGGAT AAGTCCACGG TCCTGAAGTC CACGATAGCC 
TTCCTGAAAA ATCATAATGA GGCCACCGAT CGCTCCAAGG TTTTCGAAAT ACAACAGGAC 
TGGAAGCCTG CGTTCCTCAG CAATGACGAA TACACTCACC TCATGCTGGA GTCTCTCGAT 
GGTTTTATGA TGGTCTTCAG CAGCATGGGC TCCATCTTTT ACGCCTCGGA AAGTATTACC 
TCCCAGCTGG GCTATCTGCC GCAAGATTTG TACAACATGA CCATTTATGA CCTGGCCTAT 
GAGATGGACC ATGAGGCGCT GCTGAATATC TTTATGAATC CCACACCGGT AATCGAGCCC 
AGACAGACGG ACATAAGTTC CAGTAACCAG ATCACATTCT ACACTCATTT GAGACGCGGC 
GGGATGGAGA AAGTGGATGC CAATGCCTAC GAGTTGGTCA AATTCGTGGG CTACTTTCGT 
AACGATACCA ATACTTCGAC GGGTTCCAGT TCGGAGGTTT CGAATGGCTC AAATGGACAA 
CCTGCGGTGC TGCCGCGCAT CTTCCAGCAG AATCCCAATG CCGAGGTGGA CAAAAAGCTG 
GTTTTCGTGG GCACTGGACG CGTACAGAAT CCCCAACTGA TCAGGGAAAT GAGCATTATT 
GATCCCACAA GTAATGAGTT CACTTCGAAA CACAGCATGG AGTGGAAGTT TTTGTTTCTG 
GATCACCGTG CTCCTCCGAT CATTGGCTAT ATGCCCTTCG AGGTTCTGGG GACGTCCGGC 
TATGATTATT ACCACTTTGA TGATCTGGAC AGTATTGTGG CGTGCCATGA AGAGCTAAGG 
CAAACGGGTG AGGGCAAGTC TTGCTACTAC CGATTCCTTA CGAAGGGCCA ACAGTGGATA 
TGGTTGCAAA CGGACTACTA TGTGAGCTAT CACCAGTTCA ATTCCAAACC AGACTACGTG 
GTCTGCACCC ATAAGGTTGT CAGCTATGCC GAGGTTCTGA AGGATAGTCG AAAGGAGGGC 
CAAAAATCGG GAAATAGCAA CAGCATCACC AACAATGGGA GCAGTAAGGT CATAGCCTCC 
ACGGGAACTA GCAGCAAATC CGCATCTGCC ACCACAACAC TGAGGGACTT CGAGCTGAGC 
AGCCAAAATC TGGATAGCAC ATTGCTCGGA AATAGTCTGG CCAGTTTGGG CACTGAAACA 
GCAGCCACTT CACCCGCCGT GGACTCGTCG CCCATGTGGT CAGCGTCGGC TGTCCAACCG 
TCGGGATCAT GCCAAATAAA TCCCCTGAAG ACATCGCGTC CCGCTTCGAG TTATGGCAAT 
ATCAGCTCCA CAGGAATATC GCCGAAGGCC AAACGAAAGT GCTACTTCTA CAACAACCGG 
GGGAATGACT CGGATTCAAC GTCCATGTCG ACGGATTCGG TCACCAGCCG GCAGTCCATG 
ATGACGCACG TCAGTTCGCA AAGCCAACGG CAGCGATCGC ATCACCGAGA ACACCATCGA 
GAAAATCATC ACAATCAGTC ACATCATCAC ATGCAGCAAC AGCAGCAGCA TCAGAACCAG 
CAGCAGCAAC ATCAGCAGCA CCAGCAACTG CAACAGCAGC TGCAACATAC AGTGGGTACT 
CCGAAAATGG TGCCTCTCCT GCCGATTGCG TCCACACAAA TAATGGCTGG AAATGCGTGT 
CAGTTTCCCC AACCCGCCTA TCCGCTTGCT AGTCCCCAAC TGGTGGCACC GACATTTCTT 
GAGCCGCCGC AATATCTCAC CGCCATTCCC ATGCAGCCAG TGATCGCTCC GTTTCCGGTG 
GCGCCAGTGC TTTCCCCACT TCCGGTGCAG TCACAAACGG ATATGCTGCC CGATACGGTG 
GTTATGACGC CAACACAGAG TCAGTTGCAG GATCAATTGC AGCGGAAGCA CGACGAACTG 
CAAAAACTGA TCCTCCAGCA ACAGAATGAG CTGAGGATCG TATCAGAGCA GCTCCTCCTA 
TCCCGCTACA CATATCTGCA GCCCATGATG TCGATGGGAT TCGCACCTGG CAACATGACA 
GCAGCAGCGG TGGGTAATTT GGGAGCCAGT GGCCAACGTG GACTGAATTT CACCGGCAGC 
AATGCCGTGC AGCCGCAATT CAATCAGTAT GGATTTGCAC TGAACTCGGA GCAGATGCTT 
AACCAGCAGG ATCAGCAGAT GATGATGCAG CAGCAGCAGA ATCTGCACAC GCAGCACCAG 
CACAATCTCC AGCAGCAACA TCAGAGCCAC TCACAGTTGC AGCAACATAC CCAGCAGCAA 
CATCAGCAGC AGCAGCAACA ACAGCAACAA CAGCAGCAAC AGCAGCAGCA GCAACAGCAA 
CAACAGCAGC AGCAACAACA GCAGCAACAG CAGCAGCAAC AGTTGCAGCT GCAACAACAA 
AATGATATTT TACTGCGTGA GGATATCGAT GATATAGACG CCTTTCTCAA TCTGTCCCCC 
CTGCACTCAT TGGGCTCGCA ATCCACCATT AATCCCTTCA ACTCCAGCAG CAATAACAAC 
AATCAAAGCT ACAATGGAGG CAGTAATCTG AATAATGGCA ACCAGAACAA CAACAATCGC 
AGTTCAAATC CTCCGCAAAA TAATAACGAG GACTCGCTGT TGTCCTGCAT GCAGATGGCC 
ACCGAATCGA GTCCTTCGAT CAACTTTCAC ATGGGCATCA GCGATGATGG CTCCGAAACG 
CAGAGCGAGG ACAACAAGAT GATGCACACT TCCGGCAGCA ATCTGGTGCA GCAGCAACAG 
CAGCAGCAGC AACAACAACA AATCCTGCAG CAGCATCAAC AGCAGTCAAA TAGCTTCTTC 
AGTTCGAATC CCTTTCTCAA CAGTCAGAAT CAGAATCAAA ACCAATTGCC AAACGATCTG 
GAAATATTGC CATACCAGAT GTCCCAAGAG CAATCACAGA ATCTATTCAA CTCGCCACAC 
ACAGCCCCAG GCAGTAGTCA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014576.1
CDS	371..3454
Translation	MDDESDDKDDTKSFLCRKSRNLSEKKRRDQFNSLVNDLSALISTSSRKMDKSTVLKSTIAFLKNHNEATDRSKVFEIQQDWKPAFLSNDEYTHLMLESLDGFMMVFSSMGSIFYASESITSQLGYLPQDLYNMTIYDLAYEMDHEALLNIFMNPTPVIEPRQTDISSSNQITFYTHLRRGGMEKVDANAYELVKFVGYFRNDTNTSTGSSSEVSNGSNGQPAVLPRIFQQNPNAEVDKKLVFVGTGRVQNPQLIREMSIIDPTSNEFTSKHSMEWKFLFLDHRAPPIIGYMPFEVLGTSGYDYYHFDDLDSIVACHEELRQTGEGKSCYYRFLTKGQQWIWLQTDYYVSYHQFNSKPDYVVCTHKVVSYAEVLKDSRKEGQKSGNSNSITNNGSSKVIASTGTSSKSASATTTLRDFELSSQNLDSTLLGNSLASLGTETAATSPAVDSSPMWSASAVQPSGSCQINPLKTSRPASSYGNISSTGISPKAKRKCYFYNNRGNDSDSTSMSTDSVTSRQSMMTHVSSQSQRQRSHHREHHRENHHNQSHHHMQQQQQHQNQQQQHQQHQQLQQQLQHTVGTPKMVPLLPIASTQIMAGNACQFPQPAYPLASPQLVAPTFLEPPQYLTAIPMQPVIAPFPVAPVLSPLPVQSQTDMLPDTVVMTPTQSQLQDQLQRKHDELQKLILQQQNELRIVSEQLLLSRYTYLQPMMSMGFAPGNMTAAAVGNLGASGQRGLNFTGSNAVQPQFNQYGFALNSEQMLNQQDQQMMMQQQQNLHTQHQHNLQQQHQSHSQLQQHTQQQHQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQLQLQQQNDILLREDIDDIDAFLNLSPLHSLGSQSTINPFNSSSNNNNQSYNGGSNLNNGNQNNNNRSSNPPQNNNEDSLLSCMQMATESSPSINFHMGISDDGSETQSEDNKMMHTSGSNLVQQQQQQQQQQQILQQHQQQSNSFFSSNPFLNSQNQNQNQLPNDLEILPYQMSQEQSQNLFNSPHTAPGSSQ

Target ORF information:

RefSeq Version	NM_001014576.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster clock, transcript variant D (Clk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014576.2

ORF Insert Sequence:

ATGGACGACG AGAGCGACGA CAAGGATGAT ACAAAAAGCT TCCTTTGCAG GAAATCGCGT 
AATCTCAGCG AGAAGAAGCG ACGAGATCAG TTCAACTCGC TGGTCAACGA TCTCAGCGCC 
TTGATATCCA CATCCAGCAG AAAGATGGAT AAGTCCACGG TCCTGAAGTC CACGATAGCC 
TTCCTGAAAA ATCATAATGA GGCCACCGAT CGCTCCAAGG TTTTCGAAAT ACAACAGGAC 
TGGAAGCCTG CGTTCCTCAG CAATGACGAA TACACTCACC TCATGCTGGA GTCTCTCGAT 
GGTTTTATGA TGGTCTTCAG CAGCATGGGC TCCATCTTTT ACGCCTCGGA AAGTATTACC 
TCCCAGCTGG GCTATCTGCC GCAAGATTTG TACAACATGA CCATTTATGA CCTGGCCTAT 
GAGATGGACC ATGAGGCGCT GCTGAATATC TTTATGAATC CCACACCGGT AATCGAGCCC 
AGACAGACGG ACATAAGTTC CAGTAACCAG ATCACATTCT ACACTCATTT GAGACGCGGC 
GGGATGGAGA AAGTGGATGC CAATGCCTAC GAGTTGGTCA AATTCGTGGG CTACTTTCGT 
AACGATACCA ATACTTCGAC GGGTTCCAGT TCGGAGGTTT CGAATGGCTC AAATGGACAA 
CCTGCGGTGC TGCCGCGCAT CTTCCAGCAG AATCCCAATG CCGAGGTGGA CAAAAAGCTG 
GTTTTCGTGG GCACTGGACG CGTACAGAAT CCCCAACTGA TCAGGGAAAT GAGCATTATT 
GATCCCACAA GTAATGAGTT CACTTCGAAA CACAGCATGG AGTGGAAGTT TTTGTTTCTG 
GATCACCGTG CTCCTCCGAT CATTGGCTAT ATGCCCTTCG AGGTTCTGGG GACGTCCGGC 
TATGATTATT ACCACTTTGA TGATCTGGAC AGTATTGTGG CGTGCCATGA AGAGCTAAGG 
CAAACGGGTG AGGGCAAGTC TTGCTACTAC CGATTCCTTA CGAAGGGCCA ACAGTGGATA 
TGGTTGCAAA CGGACTACTA TGTGAGCTAT CACCAGTTCA ATTCCAAACC AGACTACGTG 
GTCTGCACCC ATAAGGTTGT CAGCTATGCC GAGGTTCTGA AGGATAGTCG AAAGGAGGGC 
CAAAAATCGG GAAATAGCAA CAGCATCACC AACAATGGGA GCAGTAAGGT CATAGCCTCC 
ACGGGAACTA GCAGCAAATC CGCATCTGCC ACCACAACAC TGAGGGACTT CGAGCTGAGC 
AGCCAAAATC TGGATAGCAC ATTGCTCGGA AATAGTCTGG CCAGTTTGGG CACTGAAACA 
GCAGCCACTT CACCCGCCGT GGACTCGTCG CCCATGTGGT CAGCGTCGGC TGTCCAACCG 
TCGGGATCAT GCCAAATAAA TCCCCTGAAG ACATCGCGTC CCGCTTCGAG TTATGGCAAT 
ATCAGCTCCA CAGGAATATC GCCGAAGGCC AAACGAAAGT GCTACTTCTA CAACAACCGG 
GGGAATGACT CGGATTCAAC GTCCATGTCG ACGGATTCGG TCACCAGCCG GCAGTCCATG 
ATGACGCACG TCAGTTCGCA AAGCCAACGG CAGCGATCGC ATCACCGAGA ACACCATCGA 
GAAAATCATC ACAATCAGTC ACATCATCAC ATGCAGCAAC AGCAGCAGCA TCAGAACCAG 
CAGCAGCAAC ATCAGCAGCA CCAGCAACTG CAACAGCAGC TGCAACATAC AGTGGGTACT 
CCGAAAATGG TGCCTCTCCT GCCGATTGCG TCCACACAAA TAATGGCTGG AAATGCGTGT 
CAGTTTCCCC AACCCGCCTA TCCGCTTGCT AGTCCCCAAC TGGTGGCACC GACATTTCTT 
GAGCCGCCGC AATATCTCAC CGCCATTCCC ATGCAGCCAG TGATCGCTCC GTTTCCGGTG 
GCGCCAGTGC TTTCCCCACT TCCGGTGCAG TCACAAACGG ATATGCTGCC CGATACGGTG 
GTTATGACGC CAACACAGAG TCAGTTGCAG GATCAATTGC AGCGGAAGCA CGACGAACTG 
CAAAAACTGA TCCTCCAGCA ACAGAATGAG CTGAGGATCG TATCAGAGCA GCTCCTCCTA 
TCCCGCTACA CATATCTGCA GCCCATGATG TCGATGGGAT TCGCACCTGG CAACATGACA 
GCAGCAGCGG TGGGTAATTT GGGAGCCAGT GGCCAACGTG GACTGAATTT CACCGGCAGC 
AATGCCGTGC AGCCGCAATT CAATCAGTAT GGATTTGCAC TGAACTCGGA GCAGATGCTT 
AACCAGCAGG ATCAGCAGAT GATGATGCAG CAGCAGCAGA ATCTGCACAC GCAGCACCAG 
CACAATCTCC AGCAGCAACA TCAGAGCCAC TCACAGTTGC AGCAACATAC CCAGCAGCAA 
CATCAGCAGC AGCAGCAACA ACAGCAACAA CAGCAGCAAC AGCAGCAGCA GCAACAGCAA 
CAACAGCAGC AGCAACAACA GCAGCAACAG CAGCAGCAAC AGTTGCAGCT GCAACAACAA 
AATGATATTT TACTGCGTGA GGATATCGAT GATATAGACG CCTTTCTCAA TCTGTCCCCC 
CTGCACTCAT TGGGCTCGCA ATCCACCATT AATCCCTTCA ACTCCAGCAG CAATAACAAC 
AATCAAAGCT ACAATGGAGG CAGTAATCTG AATAATGGCA ACCAGAACAA CAACAATCGC 
AGTTCAAATC CTCCGCAAAA TAATAACGAG GACTCGCTGT TGTCCTGCAT GCAGATGGCC 
ACCGAATCGA GTCCTTCGAT CAACTTTCAC ATGGGCATCA GCGATGATGG CTCCGAAACG 
CAGAGCGAGG ACAACAAGAT GATGCACACT TCCGGCAGCA ATCTGGTGCA GCAGCAACAG 
CAGCAGCAGC AACAACAACA AATCCTGCAG CAGCATCAAC AGCAGTCAAA TAGCTTCTTC 
AGTTCGAATC CCTTTCTCAA CAGTCAGAAT CAGAATCAAA ACCAATTGCC AAACGATCTG 
GAAATATTGC CATACCAGAT GTCCCAAGAG CAATCACAGA ATCTATTCAA CTCGCCACAC 
ACAGCCCCAG GCAGTAGTCA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Clk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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