ltl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ltl
Entrez Gene ID	38845
Full Name	larval translucida
Synonyms	CG14836,CG32372,CG7545,CT15760,Dmel\CG32372,F,anon-EST:CL1a11,l(3)tr,lincRNA.S4366
General protein information	Preferred Names larval translucida Names CG32372 gene product from transcript CG32372-RB CG32372-PA CG32372-PB lethal (3) translucida lethal translucida ltl-PA ltl-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66A3-66A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_168218.2	NP_729265.1	larval translucida, isoform A
NM_001274595.1	NP_001261524.1	larval translucida, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005962	XP_316002.4
Drosophila melanogaster (fruit fly)	ltl	NP_001261524.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ltl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ltl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17810	NM_001274595.1 Latest version!	Drosophila melanogaster larval translucida (ltl), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa17810	NM_168218.2 Latest version!	Drosophila melanogaster larval translucida (ltl), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ltl ( NM_168218.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729265.1 Drosophila melanogaster larval translucida (ltl), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17810
Clone ID Related Accession (Same CDS sequence)	NM_168218.2 , NM_001274595.1
Accession Version	NM_168218.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	larval translucida, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168218.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTTCCC TCGTGTACAC CGTCTTTCAC CTGGCCTCGC TGCAATGTTC CGTGCGTCCG 
GAGATCTCGC CCTGCACCTG CGAAACGGGC AAAGCCTGGA ATCATGTGGA GCTCTCCTGC 
GAGAAGTTGG AATCCTTCAA CGCCGTCGTG GACAGCCTGG CCAATAAGCT CAATGCCGAC 
ACCAATATCG ACCTAAAGAT CACGCACTCG CAACTCGACG ACCTGGAAAT GCGCTCGTTT 
ACGGATATGA ACTTCAACTT GTACAAGCTA CGGATGCAGT GGAACAGTCT AAAATCGCTG 
CCCGAGGTGC CGTTCCGCGG GTTATCGAAT GTCACCTACC TAAGCATCGG CGACAACGAC 
CTCGACGAGA TCCCGAAGCA CGCCCTGAGC CACATGCCGA GCCTTTTGAC TCTGGACATT 
GGGCGGTGCA AGATACGGGC GGTGCAGCAG GAGGACTTCC GGGGCATACA GCGGGTAACG 
AATCTGATTC TGGTGAGCAA CATCATTACG CGCCTGGACC GAGGATCCTT TCCCAAGAGC 
CTCCTCATCC TGCATCTGGG TAGAAACCAA CTGGAGTCGC TGAATGGCTC GCTTCACGAT 
CTGCACAACT TGGAGTCTCT GTTCATCAAT GCGAATAATA TCACATCGCT GGATGATGAG 
CTACCCGATG GAGGGCAGTT GCGCCTCCTG ATGGCTCACA ATAATAGGTT GGAACGCCTG 
CCGGCCAATA TGGCCGGTAT GCACAGCCTG GAGACGGTCC ACATACACTG CAACCAGCTG 
AGATCCTTCG ACCGTGTACT TCGCAATGCC GTCAATCTGT CCGAGGTCAT GGCCGATAAC 
AATGAGCTGG AGTACCTGGC CCAGGATGAA TTCGCCTCCT GCTCCAAGGT GGAGACCCTG 
CAGATGGGCT GCAATCACAT CAAGTCGCTC AACTCCTCGC TACTGCCCAT CCTCAAGCTG 
AAGAATGCCA ACTTCTCGTT CAACGACATC GAAGAGTTCT CCATGGCGGA ACTGCACGGA 
TTGCGATCGC TGAAGACACT CCAGCTGTCC AGTAATCGCA TCCAACGACT GCTGCCCGAT 
CCCCGGGGAG TGCAGGAGCT GATGCTTGTC AATCTGGATC TGGATAACAA TAGAATCGAC 
TCTCTGAATG GAGCTTTGGC TGGCTTGGGC AATCTTCGGA TACTCAATTT GGCTGGAAAC 
CGATTGGAAC ATCTCCAGGT GGGCGATTTC GATGGCATGA TTCGATTGGA CATCCTCGAC 
CTGACCGGGA ATCAGCTAGC GGAGCTCAAG CCCTTGGAAA TGACTTTGTT GCCCAGCCTG 
AAAATCCTTA AGGTTGCGTA CAACAATATC ACCAAGCTGG AACAGGACTT CAAGGGACTG 
CCCGTGCTTT GCCAGGCCAA CTTGACCAAT AACCAGATTT CGACCATATC GAGTGAACTC 
GTCACCAACA CGCGCTGCAA AAATCACAAT GTTCCCGGCA AACTGGAGAT ACATCTAGAT 
GACAATCCCA TAATGTGTGA CGTGGGATTG AACGAGCTGT GTCGATTGAT GGCCGTTCAG 
GAGGCACGAA TTCGTGGTCG ATCCCAGTGC TTTGAGAATG ACCAGGAGGT GTGCACCGTG 
CTCCCGATGC TATACAATGT GAACCTGCCC ATAATGGTGA CCAATCTAAA GTTGACGGGC 
CGCGAGGTTC CAAAGCCGAT GGTTCGGGTG ATCGTACCAT CGCTGATCAA GGCCAATAAT 
GAGCTATTAC CGCCACTTAT TGCCACTCTG GGTAATCCAG TGCTCATTAG CACCGATTTG 
GTCAATCCGG TGATATCGCC ACTACCACCA CCACTTCTGC TGGCCACCAC AACGCCGCCA 
CCGCCTCTGC CACTTCCTGT GGAATCGGAA GTTGAGAAGA ATGAGTCTAC CACTGCGAAT 
CCAGTGCCAA CCAATTACAC ACAGGAGACC ACGACAACCA CAAGTACCAC AACGACGACA 
ACAACAACGG AGACCAGTAG TCAGGTGGCT CCCATCGAGA TTATTACGAC TACAGCACCC 
ACGACGACTA CCACCAGCAC CACAACACCG AAACCCAATG AAACGCTGCC CGTGATTGTG 
GAACTCCAGG ATCCCAATCC ACCGGACACT ACAGTCAATC AAACTGCCCT CGTTCCGCCA 
GTGGTTCTAG ATCCCCTGCA GCAGGATCTG GAAAGGGAGA GGGAACGCGA AAGGGAGCGA 
GAACTAGAGC GGGATCTGGA CCACGAGGCG GTGGCCAAGA CCGAGTACGA GACGGTCGAG 
TACATCCCCA ATCTGGTGCA GCCTCCAGTG GGCAGTGATG CACTGACGCC GCCTCCGAGC 
AAGGCGAATG CGCTGGAGGA GGACTCCGAG TCGGTGCACT CTAACTCAGT GCACGCGGAG 
TATCCCCACG CGGCGCAGAG CCTCCAGATT CCCGAGGAAC CGCCGGAGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729265.1
CDS	203..2656
Translation	MTSLVYTVFHLASLQCSVRPEISPCTCETGKAWNHVELSCEKLESFNAVVDSLANKLNADTNIDLKITHSQLDDLEMRSFTDMNFNLYKLRMQWNSLKSLPEVPFRGLSNVTYLSIGDNDLDEIPKHALSHMPSLLTLDIGRCKIRAVQQEDFRGIQRVTNLILVSNIITRLDRGSFPKSLLILHLGRNQLESLNGSLHDLHNLESLFINANNITSLDDELPDGGQLRLLMAHNNRLERLPANMAGMHSLETVHIHCNQLRSFDRVLRNAVNLSEVMADNNELEYLAQDEFASCSKVETLQMGCNHIKSLNSSLLPILKLKNANFSFNDIEEFSMAELHGLRSLKTLQLSSNRIQRLLPDPRGVQELMLVNLDLDNNRIDSLNGALAGLGNLRILNLAGNRLEHLQVGDFDGMIRLDILDLTGNQLAELKPLEMTLLPSLKILKVAYNNITKLEQDFKGLPVLCQANLTNNQISTISSELVTNTRCKNHNVPGKLEIHLDDNPIMCDVGLNELCRLMAVQEARIRGRSQCFENDQEVCTVLPMLYNVNLPIMVTNLKLTGREVPKPMVRVIVPSLIKANNELLPPLIATLGNPVLISTDLVNPVISPLPPPLLLATTTPPPPLPLPVESEVEKNESTTANPVPTNYTQETTTTTSTTTTTTTTETSSQVAPIEIITTTAPTTTTTSTTTPKPNETLPVIVELQDPNPPDTTVNQTALVPPVVLDPLQQDLERERERERERELERDLDHEAVAKTEYETVEYIPNLVQPPVGSDALTPPPSKANALEEDSESVHSNSVHAEYPHAAQSLQIPEEPPEE

Target ORF information:

RefSeq Version	NM_168218.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster larval translucida (ltl), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168218.2

ORF Insert Sequence:

ATGACTTCCC TCGTGTACAC CGTCTTTCAC CTGGCCTCGC TGCAATGTTC CGTGCGTCCG 
GAGATCTCGC CCTGCACCTG CGAAACGGGC AAAGCCTGGA ATCATGTGGA GCTCTCCTGC 
GAGAAGTTGG AATCCTTCAA CGCCGTCGTG GACAGCCTGG CCAATAAGCT CAATGCCGAC 
ACCAATATCG ACCTAAAGAT CACGCACTCG CAACTCGACG ACCTGGAAAT GCGCTCGTTT 
ACGGATATGA ACTTCAACTT GTACAAGCTA CGGATGCAGT GGAACAGTCT AAAATCGCTG 
CCCGAGGTGC CGTTCCGCGG GTTATCGAAT GTCACCTACC TAAGCATCGG CGACAACGAC 
CTCGACGAGA TCCCGAAGCA CGCCCTGAGC CACATGCCGA GCCTTTTGAC TCTGGACATT 
GGGCGGTGCA AGATACGGGC GGTGCAGCAG GAGGACTTCC GGGGCATACA GCGGGTAACG 
AATCTGATTC TGGTGAGCAA CATCATTACG CGCCTGGACC GAGGATCCTT TCCCAAGAGC 
CTCCTCATCC TGCATCTGGG TAGAAACCAA CTGGAGTCGC TGAATGGCTC GCTTCACGAT 
CTGCACAACT TGGAGTCTCT GTTCATCAAT GCGAATAATA TCACATCGCT GGATGATGAG 
CTACCCGATG GAGGGCAGTT GCGCCTCCTG ATGGCTCACA ATAATAGGTT GGAACGCCTG 
CCGGCCAATA TGGCCGGTAT GCACAGCCTG GAGACGGTCC ACATACACTG CAACCAGCTG 
AGATCCTTCG ACCGTGTACT TCGCAATGCC GTCAATCTGT CCGAGGTCAT GGCCGATAAC 
AATGAGCTGG AGTACCTGGC CCAGGATGAA TTCGCCTCCT GCTCCAAGGT GGAGACCCTG 
CAGATGGGCT GCAATCACAT CAAGTCGCTC AACTCCTCGC TACTGCCCAT CCTCAAGCTG 
AAGAATGCCA ACTTCTCGTT CAACGACATC GAAGAGTTCT CCATGGCGGA ACTGCACGGA 
TTGCGATCGC TGAAGACACT CCAGCTGTCC AGTAATCGCA TCCAACGACT GCTGCCCGAT 
CCCCGGGGAG TGCAGGAGCT GATGCTTGTC AATCTGGATC TGGATAACAA TAGAATCGAC 
TCTCTGAATG GAGCTTTGGC TGGCTTGGGC AATCTTCGGA TACTCAATTT GGCTGGAAAC 
CGATTGGAAC ATCTCCAGGT GGGCGATTTC GATGGCATGA TTCGATTGGA CATCCTCGAC 
CTGACCGGGA ATCAGCTAGC GGAGCTCAAG CCCTTGGAAA TGACTTTGTT GCCCAGCCTG 
AAAATCCTTA AGGTTGCGTA CAACAATATC ACCAAGCTGG AACAGGACTT CAAGGGACTG 
CCCGTGCTTT GCCAGGCCAA CTTGACCAAT AACCAGATTT CGACCATATC GAGTGAACTC 
GTCACCAACA CGCGCTGCAA AAATCACAAT GTTCCCGGCA AACTGGAGAT ACATCTAGAT 
GACAATCCCA TAATGTGTGA CGTGGGATTG AACGAGCTGT GTCGATTGAT GGCCGTTCAG 
GAGGCACGAA TTCGTGGTCG ATCCCAGTGC TTTGAGAATG ACCAGGAGGT GTGCACCGTG 
CTCCCGATGC TATACAATGT GAACCTGCCC ATAATGGTGA CCAATCTAAA GTTGACGGGC 
CGCGAGGTTC CAAAGCCGAT GGTTCGGGTG ATCGTACCAT CGCTGATCAA GGCCAATAAT 
GAGCTATTAC CGCCACTTAT TGCCACTCTG GGTAATCCAG TGCTCATTAG CACCGATTTG 
GTCAATCCGG TGATATCGCC ACTACCACCA CCACTTCTGC TGGCCACCAC AACGCCGCCA 
CCGCCTCTGC CACTTCCTGT GGAATCGGAA GTTGAGAAGA ATGAGTCTAC CACTGCGAAT 
CCAGTGCCAA CCAATTACAC ACAGGAGACC ACGACAACCA CAAGTACCAC AACGACGACA 
ACAACAACGG AGACCAGTAG TCAGGTGGCT CCCATCGAGA TTATTACGAC TACAGCACCC 
ACGACGACTA CCACCAGCAC CACAACACCG AAACCCAATG AAACGCTGCC CGTGATTGTG 
GAACTCCAGG ATCCCAATCC ACCGGACACT ACAGTCAATC AAACTGCCCT CGTTCCGCCA 
GTGGTTCTAG ATCCCCTGCA GCAGGATCTG GAAAGGGAGA GGGAACGCGA AAGGGAGCGA 
GAACTAGAGC GGGATCTGGA CCACGAGGCG GTGGCCAAGA CCGAGTACGA GACGGTCGAG 
TACATCCCCA ATCTGGTGCA GCCTCCAGTG GGCAGTGATG CACTGACGCC GCCTCCGAGC 
AAGGCGAATG CGCTGGAGGA GGACTCCGAG TCGGTGCACT CTAACTCAGT GCACGCGGAG 
TATCCCCACG CGGCGCAGAG CCTCCAGATT CCCGAGGAAC CGCCGGAGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ltl ( NM_001274595.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261524.1 Drosophila melanogaster larval translucida (ltl), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17810
Clone ID Related Accession (Same CDS sequence)	NM_168218.2 , NM_001274595.1
Accession Version	NM_001274595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	larval translucida, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTTCCC TCGTGTACAC CGTCTTTCAC CTGGCCTCGC TGCAATGTTC CGTGCGTCCG 
GAGATCTCGC CCTGCACCTG CGAAACGGGC AAAGCCTGGA ATCATGTGGA GCTCTCCTGC 
GAGAAGTTGG AATCCTTCAA CGCCGTCGTG GACAGCCTGG CCAATAAGCT CAATGCCGAC 
ACCAATATCG ACCTAAAGAT CACGCACTCG CAACTCGACG ACCTGGAAAT GCGCTCGTTT 
ACGGATATGA ACTTCAACTT GTACAAGCTA CGGATGCAGT GGAACAGTCT AAAATCGCTG 
CCCGAGGTGC CGTTCCGCGG GTTATCGAAT GTCACCTACC TAAGCATCGG CGACAACGAC 
CTCGACGAGA TCCCGAAGCA CGCCCTGAGC CACATGCCGA GCCTTTTGAC TCTGGACATT 
GGGCGGTGCA AGATACGGGC GGTGCAGCAG GAGGACTTCC GGGGCATACA GCGGGTAACG 
AATCTGATTC TGGTGAGCAA CATCATTACG CGCCTGGACC GAGGATCCTT TCCCAAGAGC 
CTCCTCATCC TGCATCTGGG TAGAAACCAA CTGGAGTCGC TGAATGGCTC GCTTCACGAT 
CTGCACAACT TGGAGTCTCT GTTCATCAAT GCGAATAATA TCACATCGCT GGATGATGAG 
CTACCCGATG GAGGGCAGTT GCGCCTCCTG ATGGCTCACA ATAATAGGTT GGAACGCCTG 
CCGGCCAATA TGGCCGGTAT GCACAGCCTG GAGACGGTCC ACATACACTG CAACCAGCTG 
AGATCCTTCG ACCGTGTACT TCGCAATGCC GTCAATCTGT CCGAGGTCAT GGCCGATAAC 
AATGAGCTGG AGTACCTGGC CCAGGATGAA TTCGCCTCCT GCTCCAAGGT GGAGACCCTG 
CAGATGGGCT GCAATCACAT CAAGTCGCTC AACTCCTCGC TACTGCCCAT CCTCAAGCTG 
AAGAATGCCA ACTTCTCGTT CAACGACATC GAAGAGTTCT CCATGGCGGA ACTGCACGGA 
TTGCGATCGC TGAAGACACT CCAGCTGTCC AGTAATCGCA TCCAACGACT GCTGCCCGAT 
CCCCGGGGAG TGCAGGAGCT GATGCTTGTC AATCTGGATC TGGATAACAA TAGAATCGAC 
TCTCTGAATG GAGCTTTGGC TGGCTTGGGC AATCTTCGGA TACTCAATTT GGCTGGAAAC 
CGATTGGAAC ATCTCCAGGT GGGCGATTTC GATGGCATGA TTCGATTGGA CATCCTCGAC 
CTGACCGGGA ATCAGCTAGC GGAGCTCAAG CCCTTGGAAA TGACTTTGTT GCCCAGCCTG 
AAAATCCTTA AGGTTGCGTA CAACAATATC ACCAAGCTGG AACAGGACTT CAAGGGACTG 
CCCGTGCTTT GCCAGGCCAA CTTGACCAAT AACCAGATTT CGACCATATC GAGTGAACTC 
GTCACCAACA CGCGCTGCAA AAATCACAAT GTTCCCGGCA AACTGGAGAT ACATCTAGAT 
GACAATCCCA TAATGTGTGA CGTGGGATTG AACGAGCTGT GTCGATTGAT GGCCGTTCAG 
GAGGCACGAA TTCGTGGTCG ATCCCAGTGC TTTGAGAATG ACCAGGAGGT GTGCACCGTG 
CTCCCGATGC TATACAATGT GAACCTGCCC ATAATGGTGA CCAATCTAAA GTTGACGGGC 
CGCGAGGTTC CAAAGCCGAT GGTTCGGGTG ATCGTACCAT CGCTGATCAA GGCCAATAAT 
GAGCTATTAC CGCCACTTAT TGCCACTCTG GGTAATCCAG TGCTCATTAG CACCGATTTG 
GTCAATCCGG TGATATCGCC ACTACCACCA CCACTTCTGC TGGCCACCAC AACGCCGCCA 
CCGCCTCTGC CACTTCCTGT GGAATCGGAA GTTGAGAAGA ATGAGTCTAC CACTGCGAAT 
CCAGTGCCAA CCAATTACAC ACAGGAGACC ACGACAACCA CAAGTACCAC AACGACGACA 
ACAACAACGG AGACCAGTAG TCAGGTGGCT CCCATCGAGA TTATTACGAC TACAGCACCC 
ACGACGACTA CCACCAGCAC CACAACACCG AAACCCAATG AAACGCTGCC CGTGATTGTG 
GAACTCCAGG ATCCCAATCC ACCGGACACT ACAGTCAATC AAACTGCCCT CGTTCCGCCA 
GTGGTTCTAG ATCCCCTGCA GCAGGATCTG GAAAGGGAGA GGGAACGCGA AAGGGAGCGA 
GAACTAGAGC GGGATCTGGA CCACGAGGCG GTGGCCAAGA CCGAGTACGA GACGGTCGAG 
TACATCCCCA ATCTGGTGCA GCCTCCAGTG GGCAGTGATG CACTGACGCC GCCTCCGAGC 
AAGGCGAATG CGCTGGAGGA GGACTCCGAG TCGGTGCACT CTAACTCAGT GCACGCGGAG 
TATCCCCACG CGGCGCAGAG CCTCCAGATT CCCGAGGAAC CGCCGGAGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261524.1
CDS	689..3142
Translation	MTSLVYTVFHLASLQCSVRPEISPCTCETGKAWNHVELSCEKLESFNAVVDSLANKLNADTNIDLKITHSQLDDLEMRSFTDMNFNLYKLRMQWNSLKSLPEVPFRGLSNVTYLSIGDNDLDEIPKHALSHMPSLLTLDIGRCKIRAVQQEDFRGIQRVTNLILVSNIITRLDRGSFPKSLLILHLGRNQLESLNGSLHDLHNLESLFINANNITSLDDELPDGGQLRLLMAHNNRLERLPANMAGMHSLETVHIHCNQLRSFDRVLRNAVNLSEVMADNNELEYLAQDEFASCSKVETLQMGCNHIKSLNSSLLPILKLKNANFSFNDIEEFSMAELHGLRSLKTLQLSSNRIQRLLPDPRGVQELMLVNLDLDNNRIDSLNGALAGLGNLRILNLAGNRLEHLQVGDFDGMIRLDILDLTGNQLAELKPLEMTLLPSLKILKVAYNNITKLEQDFKGLPVLCQANLTNNQISTISSELVTNTRCKNHNVPGKLEIHLDDNPIMCDVGLNELCRLMAVQEARIRGRSQCFENDQEVCTVLPMLYNVNLPIMVTNLKLTGREVPKPMVRVIVPSLIKANNELLPPLIATLGNPVLISTDLVNPVISPLPPPLLLATTTPPPPLPLPVESEVEKNESTTANPVPTNYTQETTTTTSTTTTTTTTETSSQVAPIEIITTTAPTTTTTSTTTPKPNETLPVIVELQDPNPPDTTVNQTALVPPVVLDPLQQDLERERERERERELERDLDHEAVAKTEYETVEYIPNLVQPPVGSDALTPPPSKANALEEDSESVHSNSVHAEYPHAAQSLQIPEEPPEE

Target ORF information:

RefSeq Version	NM_001274595.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster larval translucida (ltl), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274595.1

ORF Insert Sequence:

ATGACTTCCC TCGTGTACAC CGTCTTTCAC CTGGCCTCGC TGCAATGTTC CGTGCGTCCG 
GAGATCTCGC CCTGCACCTG CGAAACGGGC AAAGCCTGGA ATCATGTGGA GCTCTCCTGC 
GAGAAGTTGG AATCCTTCAA CGCCGTCGTG GACAGCCTGG CCAATAAGCT CAATGCCGAC 
ACCAATATCG ACCTAAAGAT CACGCACTCG CAACTCGACG ACCTGGAAAT GCGCTCGTTT 
ACGGATATGA ACTTCAACTT GTACAAGCTA CGGATGCAGT GGAACAGTCT AAAATCGCTG 
CCCGAGGTGC CGTTCCGCGG GTTATCGAAT GTCACCTACC TAAGCATCGG CGACAACGAC 
CTCGACGAGA TCCCGAAGCA CGCCCTGAGC CACATGCCGA GCCTTTTGAC TCTGGACATT 
GGGCGGTGCA AGATACGGGC GGTGCAGCAG GAGGACTTCC GGGGCATACA GCGGGTAACG 
AATCTGATTC TGGTGAGCAA CATCATTACG CGCCTGGACC GAGGATCCTT TCCCAAGAGC 
CTCCTCATCC TGCATCTGGG TAGAAACCAA CTGGAGTCGC TGAATGGCTC GCTTCACGAT 
CTGCACAACT TGGAGTCTCT GTTCATCAAT GCGAATAATA TCACATCGCT GGATGATGAG 
CTACCCGATG GAGGGCAGTT GCGCCTCCTG ATGGCTCACA ATAATAGGTT GGAACGCCTG 
CCGGCCAATA TGGCCGGTAT GCACAGCCTG GAGACGGTCC ACATACACTG CAACCAGCTG 
AGATCCTTCG ACCGTGTACT TCGCAATGCC GTCAATCTGT CCGAGGTCAT GGCCGATAAC 
AATGAGCTGG AGTACCTGGC CCAGGATGAA TTCGCCTCCT GCTCCAAGGT GGAGACCCTG 
CAGATGGGCT GCAATCACAT CAAGTCGCTC AACTCCTCGC TACTGCCCAT CCTCAAGCTG 
AAGAATGCCA ACTTCTCGTT CAACGACATC GAAGAGTTCT CCATGGCGGA ACTGCACGGA 
TTGCGATCGC TGAAGACACT CCAGCTGTCC AGTAATCGCA TCCAACGACT GCTGCCCGAT 
CCCCGGGGAG TGCAGGAGCT GATGCTTGTC AATCTGGATC TGGATAACAA TAGAATCGAC 
TCTCTGAATG GAGCTTTGGC TGGCTTGGGC AATCTTCGGA TACTCAATTT GGCTGGAAAC 
CGATTGGAAC ATCTCCAGGT GGGCGATTTC GATGGCATGA TTCGATTGGA CATCCTCGAC 
CTGACCGGGA ATCAGCTAGC GGAGCTCAAG CCCTTGGAAA TGACTTTGTT GCCCAGCCTG 
AAAATCCTTA AGGTTGCGTA CAACAATATC ACCAAGCTGG AACAGGACTT CAAGGGACTG 
CCCGTGCTTT GCCAGGCCAA CTTGACCAAT AACCAGATTT CGACCATATC GAGTGAACTC 
GTCACCAACA CGCGCTGCAA AAATCACAAT GTTCCCGGCA AACTGGAGAT ACATCTAGAT 
GACAATCCCA TAATGTGTGA CGTGGGATTG AACGAGCTGT GTCGATTGAT GGCCGTTCAG 
GAGGCACGAA TTCGTGGTCG ATCCCAGTGC TTTGAGAATG ACCAGGAGGT GTGCACCGTG 
CTCCCGATGC TATACAATGT GAACCTGCCC ATAATGGTGA CCAATCTAAA GTTGACGGGC 
CGCGAGGTTC CAAAGCCGAT GGTTCGGGTG ATCGTACCAT CGCTGATCAA GGCCAATAAT 
GAGCTATTAC CGCCACTTAT TGCCACTCTG GGTAATCCAG TGCTCATTAG CACCGATTTG 
GTCAATCCGG TGATATCGCC ACTACCACCA CCACTTCTGC TGGCCACCAC AACGCCGCCA 
CCGCCTCTGC CACTTCCTGT GGAATCGGAA GTTGAGAAGA ATGAGTCTAC CACTGCGAAT 
CCAGTGCCAA CCAATTACAC ACAGGAGACC ACGACAACCA CAAGTACCAC AACGACGACA 
ACAACAACGG AGACCAGTAG TCAGGTGGCT CCCATCGAGA TTATTACGAC TACAGCACCC 
ACGACGACTA CCACCAGCAC CACAACACCG AAACCCAATG AAACGCTGCC CGTGATTGTG 
GAACTCCAGG ATCCCAATCC ACCGGACACT ACAGTCAATC AAACTGCCCT CGTTCCGCCA 
GTGGTTCTAG ATCCCCTGCA GCAGGATCTG GAAAGGGAGA GGGAACGCGA AAGGGAGCGA 
GAACTAGAGC GGGATCTGGA CCACGAGGCG GTGGCCAAGA CCGAGTACGA GACGGTCGAG 
TACATCCCCA ATCTGGTGCA GCCTCCAGTG GGCAGTGATG CACTGACGCC GCCTCCGAGC 
AAGGCGAATG CGCTGGAGGA GGACTCCGAG TCGGTGCACT CTAACTCAGT GCACGCGGAG 
TATCCCCACG CGGCGCAGAG CCTCCAGATT CCCGAGGAAC CGCCGGAGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ltl cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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