Neos cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Neos
Entrez Gene ID	38795
Full Name	Neosin
Synonyms	BcDNA.HL05936,BcDNA:HL05936,CG8614,Dmel\CG8614,l(3)L2249
General protein information	Preferred Names Neosin Names CG8614 gene product from transcript CG8614-RA CG8614-PA CG8614-PB Neos-PA Neos-PB neosin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 65E9-65E9

mRNA and Protein(s)

mRNA	Protein	Name
NM_079231.4	NP_523955.1	neosin, isoform A
NM_001274573.1	NP_001261502.1	neosin, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Neos	NP_523955.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007689	XP_308182.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Neos gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Neos cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17773	NM_079231.4 Latest version!	Drosophila melanogaster neosin, transcript variant A (Neos), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa17773	NM_001274573.1 Latest version!	Drosophila melanogaster neosin, transcript variant B (Neos), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Neos ( NM_079231.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523955.1 Drosophila melanogaster neosin, transcript variant A (Neos), mRNA.

Price & Availability↑

CloneID	OFa17773
Clone ID Related Accession (Same CDS sequence)	NM_079231.4 , NM_001274573.1
Accession Version	NM_079231.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neosin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079231.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGATC CAGGAAGCGC CGGCTACAAC ATAACCAAGG ATCCGGCCCT GGCAAAGAGT 
CGAATCTTCT TGGGCAACCT GCCGGTTTGC ACGCGCGAGG AGCTGGTGAG CATTTGCCAG 
CCATATGGCA AAGTGCTCGG TTCGATGGTC CAGAAGAACT ACGGATTCGT GCAGTTTGAA 
ACGGAGGAGC TGGCCAATAA AGCCGCCTCC GCCCTGCACA AGTCCACTTT TAAGCAAAAC 
ATGCTCACCG TACGCAACGC CAGCATTAAA TCGAAGGCGG CCAATGCTAT CGCCAAAAGA 
AATTCAAATC AAGGAGGCCA GGTGACAGTT CAGGGTGGAC TTGTGATGTC AGCGGCGGCA 
GCGGCAGCAG GTCAACCCCT CATCAATGAT TGCGAGATCA TAGTCCAAAA TCGCGAGAAT 
ACCAAATACG CAGAATACAT TGAGGAGCGG CTAAAGAATT CCAGTCTGCG CGTGGATGTA 
CTCTTTCCCA ACGAGGACGT CTTGCTAGGC AAGGTCCTGG CCAACATCTC TTCGCGCGGC 
TGCTTGTATG CGGTATTGGT CACGCCGCAG CACGAGGAAC ACAACAGCAT CACAGTAAAC 
ATTTTGTATG GCGTACCCGC TGAGCATCGC AACATGCCGC TGGAGGACGC CATCACCCTG 
ATCTCCACCG ATTTTAGGCT CAAAAAACAG CGCGACGCTG TCGTCTTACC GCCGTCAACA 
TCCATACACA AGGGACAGCG ACGACATCCC CAGGAAATGC AGGGGCTGCT GGAAAGGCTG 
GCAGACAATC ATCCATTGAC CGCGTCGCAG TACGAGGTGA TTCTTAAGTA CCTCGAAGGC 
GAACGCGAGG AGCAACTGAA GCGAGAGGTG GGCGAGGCCA ATGCTCTGGC CAAGCTAAAA 
GCTCCCGATC CAGAGATCGA GCTGCAGAAG AAGATTCTTA GCATCATGAA CAAGCCGGCA 
GTAACCGATG TCACCAGCGA GCTCATGTAT CCCACTTTCG AGGCAGTCAA GGAGGACAGA 
AGGCTAATGG AGCTACTTGC TGACGATCGA GTGTTGGCGG CCCTGGAAAG TGTTTACAAC 
TCGGATCTCA GACAGATTTT AGCCGAGTAT TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523955.1
CDS	248..1363
Translation	MSDPGSAGYNITKDPALAKSRIFLGNLPVCTREELVSICQPYGKVLGSMVQKNYGFVQFETEELANKAASALHKSTFKQNMLTVRNASIKSKAANAIAKRNSNQGGQVTVQGGLVMSAAAAAAGQPLINDCEIIVQNRENTKYAEYIEERLKNSSLRVDVLFPNEDVLLGKVLANISSRGCLYAVLVTPQHEEHNSITVNILYGVPAEHRNMPLEDAITLISTDFRLKKQRDAVVLPPSTSIHKGQRRHPQEMQGLLERLADNHPLTASQYEVILKYLEGEREEQLKREVGEANALAKLKAPDPEIELQKKILSIMNKPAVTDVTSELMYPTFEAVKEDRRLMELLADDRVLAALESVYNSDLRQILAEYL

Target ORF information:

RefSeq Version	NM_079231.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neosin, transcript variant A (Neos), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079231.4

ORF Insert Sequence:

ATGTCTGATC CAGGAAGCGC CGGCTACAAC ATAACCAAGG ATCCGGCCCT GGCAAAGAGT 
CGAATCTTCT TGGGCAACCT GCCGGTTTGC ACGCGCGAGG AGCTGGTGAG CATTTGCCAG 
CCATATGGCA AAGTGCTCGG TTCGATGGTC CAGAAGAACT ACGGATTCGT GCAGTTTGAA 
ACGGAGGAGC TGGCCAATAA AGCCGCCTCC GCCCTGCACA AGTCCACTTT TAAGCAAAAC 
ATGCTCACCG TACGCAACGC CAGCATTAAA TCGAAGGCGG CCAATGCTAT CGCCAAAAGA 
AATTCAAATC AAGGAGGCCA GGTGACAGTT CAGGGTGGAC TTGTGATGTC AGCGGCGGCA 
GCGGCAGCAG GTCAACCCCT CATCAATGAT TGCGAGATCA TAGTCCAAAA TCGCGAGAAT 
ACCAAATACG CAGAATACAT TGAGGAGCGG CTAAAGAATT CCAGTCTGCG CGTGGATGTA 
CTCTTTCCCA ACGAGGACGT CTTGCTAGGC AAGGTCCTGG CCAACATCTC TTCGCGCGGC 
TGCTTGTATG CGGTATTGGT CACGCCGCAG CACGAGGAAC ACAACAGCAT CACAGTAAAC 
ATTTTGTATG GCGTACCCGC TGAGCATCGC AACATGCCGC TGGAGGACGC CATCACCCTG 
ATCTCCACCG ATTTTAGGCT CAAAAAACAG CGCGACGCTG TCGTCTTACC GCCGTCAACA 
TCCATACACA AGGGACAGCG ACGACATCCC CAGGAAATGC AGGGGCTGCT GGAAAGGCTG 
GCAGACAATC ATCCATTGAC CGCGTCGCAG TACGAGGTGA TTCTTAAGTA CCTCGAAGGC 
GAACGCGAGG AGCAACTGAA GCGAGAGGTG GGCGAGGCCA ATGCTCTGGC CAAGCTAAAA 
GCTCCCGATC CAGAGATCGA GCTGCAGAAG AAGATTCTTA GCATCATGAA CAAGCCGGCA 
GTAACCGATG TCACCAGCGA GCTCATGTAT CCCACTTTCG AGGCAGTCAA GGAGGACAGA 
AGGCTAATGG AGCTACTTGC TGACGATCGA GTGTTGGCGG CCCTGGAAAG TGTTTACAAC 
TCGGATCTCA GACAGATTTT AGCCGAGTAT TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Neos ( NM_001274573.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261502.1 Drosophila melanogaster neosin, transcript variant B (Neos), mRNA.

Price & Availability↑

CloneID	OFa17773
Clone ID Related Accession (Same CDS sequence)	NM_079231.4 , NM_001274573.1
Accession Version	NM_001274573.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neosin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGATC CAGGAAGCGC CGGCTACAAC ATAACCAAGG ATCCGGCCCT GGCAAAGAGT 
CGAATCTTCT TGGGCAACCT GCCGGTTTGC ACGCGCGAGG AGCTGGTGAG CATTTGCCAG 
CCATATGGCA AAGTGCTCGG TTCGATGGTC CAGAAGAACT ACGGATTCGT GCAGTTTGAA 
ACGGAGGAGC TGGCCAATAA AGCCGCCTCC GCCCTGCACA AGTCCACTTT TAAGCAAAAC 
ATGCTCACCG TACGCAACGC CAGCATTAAA TCGAAGGCGG CCAATGCTAT CGCCAAAAGA 
AATTCAAATC AAGGAGGCCA GGTGACAGTT CAGGGTGGAC TTGTGATGTC AGCGGCGGCA 
GCGGCAGCAG GTCAACCCCT CATCAATGAT TGCGAGATCA TAGTCCAAAA TCGCGAGAAT 
ACCAAATACG CAGAATACAT TGAGGAGCGG CTAAAGAATT CCAGTCTGCG CGTGGATGTA 
CTCTTTCCCA ACGAGGACGT CTTGCTAGGC AAGGTCCTGG CCAACATCTC TTCGCGCGGC 
TGCTTGTATG CGGTATTGGT CACGCCGCAG CACGAGGAAC ACAACAGCAT CACAGTAAAC 
ATTTTGTATG GCGTACCCGC TGAGCATCGC AACATGCCGC TGGAGGACGC CATCACCCTG 
ATCTCCACCG ATTTTAGGCT CAAAAAACAG CGCGACGCTG TCGTCTTACC GCCGTCAACA 
TCCATACACA AGGGACAGCG ACGACATCCC CAGGAAATGC AGGGGCTGCT GGAAAGGCTG 
GCAGACAATC ATCCATTGAC CGCGTCGCAG TACGAGGTGA TTCTTAAGTA CCTCGAAGGC 
GAACGCGAGG AGCAACTGAA GCGAGAGGTG GGCGAGGCCA ATGCTCTGGC CAAGCTAAAA 
GCTCCCGATC CAGAGATCGA GCTGCAGAAG AAGATTCTTA GCATCATGAA CAAGCCGGCA 
GTAACCGATG TCACCAGCGA GCTCATGTAT CCCACTTTCG AGGCAGTCAA GGAGGACAGA 
AGGCTAATGG AGCTACTTGC TGACGATCGA GTGTTGGCGG CCCTGGAAAG TGTTTACAAC 
TCGGATCTCA GACAGATTTT AGCCGAGTAT TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261502.1
CDS	59..1174
Translation	MSDPGSAGYNITKDPALAKSRIFLGNLPVCTREELVSICQPYGKVLGSMVQKNYGFVQFETEELANKAASALHKSTFKQNMLTVRNASIKSKAANAIAKRNSNQGGQVTVQGGLVMSAAAAAAGQPLINDCEIIVQNRENTKYAEYIEERLKNSSLRVDVLFPNEDVLLGKVLANISSRGCLYAVLVTPQHEEHNSITVNILYGVPAEHRNMPLEDAITLISTDFRLKKQRDAVVLPPSTSIHKGQRRHPQEMQGLLERLADNHPLTASQYEVILKYLEGEREEQLKREVGEANALAKLKAPDPEIELQKKILSIMNKPAVTDVTSELMYPTFEAVKEDRRLMELLADDRVLAALESVYNSDLRQILAEYL

Target ORF information:

RefSeq Version	NM_001274573.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neosin, transcript variant B (Neos), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274573.1

ORF Insert Sequence:

ATGTCTGATC CAGGAAGCGC CGGCTACAAC ATAACCAAGG ATCCGGCCCT GGCAAAGAGT 
CGAATCTTCT TGGGCAACCT GCCGGTTTGC ACGCGCGAGG AGCTGGTGAG CATTTGCCAG 
CCATATGGCA AAGTGCTCGG TTCGATGGTC CAGAAGAACT ACGGATTCGT GCAGTTTGAA 
ACGGAGGAGC TGGCCAATAA AGCCGCCTCC GCCCTGCACA AGTCCACTTT TAAGCAAAAC 
ATGCTCACCG TACGCAACGC CAGCATTAAA TCGAAGGCGG CCAATGCTAT CGCCAAAAGA 
AATTCAAATC AAGGAGGCCA GGTGACAGTT CAGGGTGGAC TTGTGATGTC AGCGGCGGCA 
GCGGCAGCAG GTCAACCCCT CATCAATGAT TGCGAGATCA TAGTCCAAAA TCGCGAGAAT 
ACCAAATACG CAGAATACAT TGAGGAGCGG CTAAAGAATT CCAGTCTGCG CGTGGATGTA 
CTCTTTCCCA ACGAGGACGT CTTGCTAGGC AAGGTCCTGG CCAACATCTC TTCGCGCGGC 
TGCTTGTATG CGGTATTGGT CACGCCGCAG CACGAGGAAC ACAACAGCAT CACAGTAAAC 
ATTTTGTATG GCGTACCCGC TGAGCATCGC AACATGCCGC TGGAGGACGC CATCACCCTG 
ATCTCCACCG ATTTTAGGCT CAAAAAACAG CGCGACGCTG TCGTCTTACC GCCGTCAACA 
TCCATACACA AGGGACAGCG ACGACATCCC CAGGAAATGC AGGGGCTGCT GGAAAGGCTG 
GCAGACAATC ATCCATTGAC CGCGTCGCAG TACGAGGTGA TTCTTAAGTA CCTCGAAGGC 
GAACGCGAGG AGCAACTGAA GCGAGAGGTG GGCGAGGCCA ATGCTCTGGC CAAGCTAAAA 
GCTCCCGATC CAGAGATCGA GCTGCAGAAG AAGATTCTTA GCATCATGAA CAAGCCGGCA 
GTAACCGATG TCACCAGCGA GCTCATGTAT CCCACTTTCG AGGCAGTCAA GGAGGACAGA 
AGGCTAATGG AGCTACTTGC TGACGATCGA GTGTTGGCGG CCCTGGAAAG TGTTTACAAC 
TCGGATCTCA GACAGATTTT AGCCGAGTAT TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Neos cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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