melt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	melt
Entrez Gene ID	38785
Full Name	melted
Synonyms	1441/14,CG8624,Dmel\CG8624,Melt,Q9VS24_DROME,anon-WO03014152.10,dMelt
General protein information	Preferred Names melted Names CG8624 gene product from transcript CG8624-RD CG8624-PB CG8624-PC CG8624-PD melt-PB melt-PC melt-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 65E4-65E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001014572.4	NP_001014572.1	melted, isoform B
NM_079229.4	NP_523953.2	melted, isoform C
NM_001274566.1	NP_001261495.1	melted, isoform D

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007472	XP_308399.4
Caenorhabditis elegans (roundworm)	K10B4.3	NP_493664.2
Canis lupus familiaris (dog)	VEPH1	XP_005634666.1
Rattus norvegicus (Norway rat)	Veph1	NP_001014193.1
Gallus gallus (chicken)	VEPH1	XP_003641830.1
Drosophila melanogaster (fruit fly)	melt	NP_523953.2
Homo sapiens (human)	VEPH1	NP_001161384.1
Mus musculus (house mouse)	Veph1	NP_665819.2
	VEPH1	XP_001103348.1
Bos taurus (cattle)	VEPH1	XP_002685007.2
Xenopus tropicalis (tropical clawed frog)	veph1	XP_002933300.2
Pan troglodytes (chimpanzee)	VEPH1	XP_001152941.1
Danio rerio (zebrafish)	veph	NP_997996.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Here, we show that warts, encoding large tumor suppressor (Lats) and melted encoding a PH-domain protein, play opposite roles in defining the yR 8 or pR8 fates in Drosophila ommatidia photoreceptors.
Title: The growth regulators warts/lats and melted interact in a bistable loop to specify opposite fates in Drosophila R8 photoreceptors.

Melted as a new modulator of the insulin/PI3K signaling pathway which controls both tissue growth and metabolism
Title: Drosophila Melted modulates FOXO and TOR activity.

The following melt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the melt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17758	NM_079229.4 Latest version!	Drosophila melanogaster melted (melt), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa17759	NM_001014572.4 Latest version!	Drosophila melanogaster melted (melt), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa20624	NM_001274566.1 Latest version!	Drosophila melanogaster melted (melt), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

melt ( NM_079229.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523953.2 Drosophila melanogaster melted (melt), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa17758
Clone ID Related Accession (Same CDS sequence)	NM_079229.4
Accession Version	NM_079229.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	melted, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079229.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCACGAAC TTTTCAACAA AGTGCTCGCC AAACGCGATC TCTCAAGAGC CGGAGATCTC 
TTTTCCGTGC CGGATGCAGA TATAGTCGAC GACATCACGG AGGTGCTTTC CGAGATCAGT 
CCGATCATCT CGCACGCGGA CTATGTGAAG AACAACAATG ACCAGAGTGT GGTGGAGATC 
TGTGTGACAC GGGTGCTATC CTGCATCCGG GAAACGAAAA CTGCGGAGCG ATACTGTGCT 
GCTCTGGTGG ATCTTCTGAG GACCTGTCTG CTATGGAACC TCCAACCCTC GGGAACAACA 
AAGGAAGAGC CACCCCATGC CAAGATTGCT GCCGATATCA TCTCTAGCAT TTTCCTGAAC 
TATGACAAAA AAGAACTGAT GAAAATAGCC CTTCCCGTCG CCGTGCAGTT CCTTCCGAAG 
GGAAACCGTG AGCTATCGCG AAACTTGGCC AGTTACCTCT CACTGGCGGC CATCGATCAC 
GCCGGCTTGC TGAGCCCTCA TACGGAGTCC GTGATGGAGT CCATTCTGTC CGGAAACTAT 
GGTCTACTCC GAGTGCTTTC ACAGGTGTAC GAGGTGGCTC CGGAGGCGGT GACGCCACAT 
GCACCACTTC TCATGCCCCT GCTGCCACAG TGTGATCCGC AGGAGAGAAT GGCGGTGTTC 
CAGCTGTATC TGCTGATCGT TCAGAAGTCA CCAGAGGTTC TGGAATCTTG CGTTCCGCAA 
CTTTGTGGCT CCCTGCTCGA TAGCGACACT TCTAGCATAA CCATGCAGAT TCTGCTGAAG 
CTTTCCCAGC ACTCGCCCAG CCTTCTTGTC GACCATTTCG AGCAATTGCG CCTGGCAGCT 
AAAACCAATC CTGGAACGAT TCCGCTTTGT GCCCAGATTA TGACTACCGC GGGAATCGGG 
AGCAAGGAGA CGGCCCAGCA GGCTTTGGAC TTTGTGCTGG AGCACCTGCC CACGCAGGCA 
CTCCTGCAAC AGGAGGCCAC TCGCCTCTGT TCCGCCTATC CGGTTTTGTT CACCGACAAA 
GTACTGGCCT GCGTGCGGCA GAAGAACGCA GCTCTCAGCT CGCAACAGGA TGTGGGGAAT 
GGCTTGGCGA ACAAGACCTC TGGTGGTGTC ACCATTGTCA GCTTGAATAG CTCCAGTCCC 
AGTCCGCCGC TTAAGCCAGT TCCAGTTGGC GCACCAGCTA TTAACACCAC AATCGTCCAG 
CCGGCCAGCG TAGTCAGTGC CACGCCCACA CCACCTCCAT CCTCCGCCTC GGCGCCCATT 
GCCAGCTCCA CGCCCGTATC GGCTGCCACG CCCACTCCCC AGCATGCGGG ATACACGCGG 
CGGGTGAAGC TAGGCGATTC CCGGAGCACA GGTCGCCTGC ATCCCGCCAG CAATACGCAC 
CGGAGTGTGA CCCGGTTGAA TGTGGCCAGC GGATCCGTGG GAGGACTTCA CAAGAGCATG 
ACTCGGCTGA GTAACTCGCA GATTAACCAG CAACCTGGAG GCAGCTCCAA TGGGAATGTC 
GTGGTGCAAT CGGGGGCTAC TCCGAAGACC CCAACTGGTC TAAGCAATCC GCCTGTGACA 
CCAGTGCCGC CCTTGAGCAA CAACGTGGTC ATAACTGGAC ACAATCGACA TGGTATACCT 
GTTACCTCTG GAGGAGTGAC TGTGACCACG TCGCCCTCGA AGGTGCGACC ACACTCCCAA 
GGACCATCAA CGCTGCTCAA CTCGAGCACG GTGCTGATGA AGTACAGCAC GGATGCACTG 
AATCAATCGG TGGGCTCGAT CTCCATACCA CAGTCGGCAG CGGTGGCCAC TTTGCCATCG 
ACGCAGACAC AGAATGCGGT ATCCGTCCAT CATGCCTCGC CGGCGCTGCC CCAATCCGCC 
AGCAATGGTC ATGCCAAGTC AGTGATGAAA CTGCCAGTCA ACGGAAATAG CGAGGTAATC 
GTTTCGGGAC CCACCACCAA TGTGGCACCT CGGCGCAGCG ATAATACCAG CAGGACCCTA 
CTGAATGCCA ACAGTGTCAT GGACCAGCGG ATGAGCACCT TTGAGCCATA CCAGATTATG 
CGTGATCCTG TTCAGCAATT CTGCGAGAAG AACTTCAATT CGATCAAGTC CTACATGGAT 
GAGGTATCGC AGCATCTGCC GCCGCCCACA CGTTGCAGCA TTGAGGTTTC CAATGAATTT 
GCAGAGCGTC GTACCAAAAA GGTGGCCAAA CTGCATTTCG CCTGCCAGAT TCGTGGTCCC 
CATTGCCTGT ACTCCAAAAC TTGCTTCACG ATGCGAACTC GGAACCCCAA GACCTGGATA 
CATATGATGT TCCTGGACTT TCAAGTGCGC CATTTTGTGA AGGAGAAGTG CGTGCTTAGC 
ACGCGGGAGT CCGGCATTAG CAATCTCAAG AATATTTGGC AGATACTCAA GTGCGAGAAC 
CGCAGCTTCA CCGAGCTGGT TACCAGTCAG TTTCCTCAGG TTAAGGACCG CGAGATCCTG 
GTGAACGAGC TGCGTCACTC GGGCTTTCTG GATGTGTTCG AGGTCTCCAA GACAGACAAG 
TCCAACCCAA ACTGCAATGA GTTGGAATAC CAGTGGGGCT GCTTCCTCTG CAATCATCCG 
GACAAGGCGG TGGGCTTTCT CAATGGCAGC AATCAGCCCA TGATCGAGGG CCAGCTGAAG 
GAGAAGAAGG GCAAGTGGCG ACTATTCAGG CGCTGGCGAA CGCGCTACTT CACTTTGTCC 
GGAGCGCACT TGTCCTGCAA AGGCTCTAGC GGAGGTGAGA GCATCGATGT GAATCAGATA 
CGTTCGGTAA AGGTATCTCG TGGTGCCCGT AACATACCGA AGGCCTTCGA AATCTTCACC 
GCCGACCAGA CATTAATACT CAAGCCCAAG GACGGCAAGA ATGCCGAGGA GTGGGTGCAG 
TGCCTCAGCA TCGTGGTAGC CCACTCGCAG GCCCGAGATA ATCCCACGGC CAAGACGAAC 
AGCCTGCCCG CCCGCAGCAT AGGCAGCAGC AAGCCCTCCT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523953.2
CDS	763..3747
Translation	MHELFNKVLAKRDLSRAGDLFSVPDADIVDDITEVLSEISPIISHADYVKNNNDQSVVEICVTRVLSCIRETKTAERYCAALVDLLRTCLLWNLQPSGTTKEEPPHAKIAADIISSIFLNYDKKELMKIALPVAVQFLPKGNRELSRNLASYLSLAAIDHAGLLSPHTESVMESILSGNYGLLRVLSQVYEVAPEAVTPHAPLLMPLLPQCDPQERMAVFQLYLLIVQKSPEVLESCVPQLCGSLLDSDTSSITMQILLKLSQHSPSLLVDHFEQLRLAAKTNPGTIPLCAQIMTTAGIGSKETAQQALDFVLEHLPTQALLQQEATRLCSAYPVLFTDKVLACVRQKNAALSSQQDVGNGLANKTSGGVTIVSLNSSSPSPPLKPVPVGAPAINTTIVQPASVVSATPTPPPSSASAPIASSTPVSAATPTPQHAGYTRRVKLGDSRSTGRLHPASNTHRSVTRLNVASGSVGGLHKSMTRLSNSQINQQPGGSSNGNVVVQSGATPKTPTGLSNPPVTPVPPLSNNVVITGHNRHGIPVTSGGVTVTTSPSKVRPHSQGPSTLLNSSTVLMKYSTDALNQSVGSISIPQSAAVATLPSTQTQNAVSVHHASPALPQSASNGHAKSVMKLPVNGNSEVIVSGPTTNVAPRRSDNTSRTLLNANSVMDQRMSTFEPYQIMRDPVQQFCEKNFNSIKSYMDEVSQHLPPPTRCSIEVSNEFAERRTKKVAKLHFACQIRGPHCLYSKTCFTMRTRNPKTWIHMMFLDFQVRHFVKEKCVLSTRESGISNLKNIWQILKCENRSFTELVTSQFPQVKDREILVNELRHSGFLDVFEVSKTDKSNPNCNELEYQWGCFLCNHPDKAVGFLNGSNQPMIEGQLKEKKGKWRLFRRWRTRYFTLSGAHLSCKGSSGGESIDVNQIRSVKVSRGARNIPKAFEIFTADQTLILKPKDGKNAEEWVQCLSIVVAHSQARDNPTAKTNSLPARSIGSSKPSF

Target ORF information:

RefSeq Version	NM_079229.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster melted (melt), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079229.4

ORF Insert Sequence:

ATGCACGAAC TTTTCAACAA AGTGCTCGCC AAACGCGATC TCTCAAGAGC CGGAGATCTC 
TTTTCCGTGC CGGATGCAGA TATAGTCGAC GACATCACGG AGGTGCTTTC CGAGATCAGT 
CCGATCATCT CGCACGCGGA CTATGTGAAG AACAACAATG ACCAGAGTGT GGTGGAGATC 
TGTGTGACAC GGGTGCTATC CTGCATCCGG GAAACGAAAA CTGCGGAGCG ATACTGTGCT 
GCTCTGGTGG ATCTTCTGAG GACCTGTCTG CTATGGAACC TCCAACCCTC GGGAACAACA 
AAGGAAGAGC CACCCCATGC CAAGATTGCT GCCGATATCA TCTCTAGCAT TTTCCTGAAC 
TATGACAAAA AAGAACTGAT GAAAATAGCC CTTCCCGTCG CCGTGCAGTT CCTTCCGAAG 
GGAAACCGTG AGCTATCGCG AAACTTGGCC AGTTACCTCT CACTGGCGGC CATCGATCAC 
GCCGGCTTGC TGAGCCCTCA TACGGAGTCC GTGATGGAGT CCATTCTGTC CGGAAACTAT 
GGTCTACTCC GAGTGCTTTC ACAGGTGTAC GAGGTGGCTC CGGAGGCGGT GACGCCACAT 
GCACCACTTC TCATGCCCCT GCTGCCACAG TGTGATCCGC AGGAGAGAAT GGCGGTGTTC 
CAGCTGTATC TGCTGATCGT TCAGAAGTCA CCAGAGGTTC TGGAATCTTG CGTTCCGCAA 
CTTTGTGGCT CCCTGCTCGA TAGCGACACT TCTAGCATAA CCATGCAGAT TCTGCTGAAG 
CTTTCCCAGC ACTCGCCCAG CCTTCTTGTC GACCATTTCG AGCAATTGCG CCTGGCAGCT 
AAAACCAATC CTGGAACGAT TCCGCTTTGT GCCCAGATTA TGACTACCGC GGGAATCGGG 
AGCAAGGAGA CGGCCCAGCA GGCTTTGGAC TTTGTGCTGG AGCACCTGCC CACGCAGGCA 
CTCCTGCAAC AGGAGGCCAC TCGCCTCTGT TCCGCCTATC CGGTTTTGTT CACCGACAAA 
GTACTGGCCT GCGTGCGGCA GAAGAACGCA GCTCTCAGCT CGCAACAGGA TGTGGGGAAT 
GGCTTGGCGA ACAAGACCTC TGGTGGTGTC ACCATTGTCA GCTTGAATAG CTCCAGTCCC 
AGTCCGCCGC TTAAGCCAGT TCCAGTTGGC GCACCAGCTA TTAACACCAC AATCGTCCAG 
CCGGCCAGCG TAGTCAGTGC CACGCCCACA CCACCTCCAT CCTCCGCCTC GGCGCCCATT 
GCCAGCTCCA CGCCCGTATC GGCTGCCACG CCCACTCCCC AGCATGCGGG ATACACGCGG 
CGGGTGAAGC TAGGCGATTC CCGGAGCACA GGTCGCCTGC ATCCCGCCAG CAATACGCAC 
CGGAGTGTGA CCCGGTTGAA TGTGGCCAGC GGATCCGTGG GAGGACTTCA CAAGAGCATG 
ACTCGGCTGA GTAACTCGCA GATTAACCAG CAACCTGGAG GCAGCTCCAA TGGGAATGTC 
GTGGTGCAAT CGGGGGCTAC TCCGAAGACC CCAACTGGTC TAAGCAATCC GCCTGTGACA 
CCAGTGCCGC CCTTGAGCAA CAACGTGGTC ATAACTGGAC ACAATCGACA TGGTATACCT 
GTTACCTCTG GAGGAGTGAC TGTGACCACG TCGCCCTCGA AGGTGCGACC ACACTCCCAA 
GGACCATCAA CGCTGCTCAA CTCGAGCACG GTGCTGATGA AGTACAGCAC GGATGCACTG 
AATCAATCGG TGGGCTCGAT CTCCATACCA CAGTCGGCAG CGGTGGCCAC TTTGCCATCG 
ACGCAGACAC AGAATGCGGT ATCCGTCCAT CATGCCTCGC CGGCGCTGCC CCAATCCGCC 
AGCAATGGTC ATGCCAAGTC AGTGATGAAA CTGCCAGTCA ACGGAAATAG CGAGGTAATC 
GTTTCGGGAC CCACCACCAA TGTGGCACCT CGGCGCAGCG ATAATACCAG CAGGACCCTA 
CTGAATGCCA ACAGTGTCAT GGACCAGCGG ATGAGCACCT TTGAGCCATA CCAGATTATG 
CGTGATCCTG TTCAGCAATT CTGCGAGAAG AACTTCAATT CGATCAAGTC CTACATGGAT 
GAGGTATCGC AGCATCTGCC GCCGCCCACA CGTTGCAGCA TTGAGGTTTC CAATGAATTT 
GCAGAGCGTC GTACCAAAAA GGTGGCCAAA CTGCATTTCG CCTGCCAGAT TCGTGGTCCC 
CATTGCCTGT ACTCCAAAAC TTGCTTCACG ATGCGAACTC GGAACCCCAA GACCTGGATA 
CATATGATGT TCCTGGACTT TCAAGTGCGC CATTTTGTGA AGGAGAAGTG CGTGCTTAGC 
ACGCGGGAGT CCGGCATTAG CAATCTCAAG AATATTTGGC AGATACTCAA GTGCGAGAAC 
CGCAGCTTCA CCGAGCTGGT TACCAGTCAG TTTCCTCAGG TTAAGGACCG CGAGATCCTG 
GTGAACGAGC TGCGTCACTC GGGCTTTCTG GATGTGTTCG AGGTCTCCAA GACAGACAAG 
TCCAACCCAA ACTGCAATGA GTTGGAATAC CAGTGGGGCT GCTTCCTCTG CAATCATCCG 
GACAAGGCGG TGGGCTTTCT CAATGGCAGC AATCAGCCCA TGATCGAGGG CCAGCTGAAG 
GAGAAGAAGG GCAAGTGGCG ACTATTCAGG CGCTGGCGAA CGCGCTACTT CACTTTGTCC 
GGAGCGCACT TGTCCTGCAA AGGCTCTAGC GGAGGTGAGA GCATCGATGT GAATCAGATA 
CGTTCGGTAA AGGTATCTCG TGGTGCCCGT AACATACCGA AGGCCTTCGA AATCTTCACC 
GCCGACCAGA CATTAATACT CAAGCCCAAG GACGGCAAGA ATGCCGAGGA GTGGGTGCAG 
TGCCTCAGCA TCGTGGTAGC CCACTCGCAG GCCCGAGATA ATCCCACGGC CAAGACGAAC 
AGCCTGCCCG CCCGCAGCAT AGGCAGCAGC AAGCCCTCCT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

melt ( NM_001014572.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014572.1 Drosophila melanogaster melted (melt), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17759
Clone ID Related Accession (Same CDS sequence)	NM_001014572.4
Accession Version	NM_001014572.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	melted, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001014572.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCACGAAC TTTTCAACAA AGTGCTCGCC AAACGCGATC TCTCAAGAGC CGGAGATCTC 
TTTTCCGTGC CGGATGCAGA TATAGTCGAC GACATCACGG AGGTGCTTTC CGAGATCAGT 
CCGATCATCT CGCACGCGGA CTATGTGAAG AACAACAATG ACCAGAGTGT GGTGGAGATC 
TGTGTGACAC GGGTGCTATC CTGCATCCGG GAAACGAAAA CTGCGGAGCG ATACTGTGCT 
GCTCTGGTGG ATCTTCTGAG GACCTGTCTG CTATGGAACC TCCAACCCTC GGGAACAACA 
AAGGAAGAGC CACCCCATGC CAAGATTGCT GCCGATATCA TCTCTAGCAT TTTCCTGAAC 
TATGACAAAA AAGAACTGAT GAAAATAGCC CTTCCCGTCG CCGTGCAGTT CCTTCCGAAG 
GGAAACCGTG AGCTATCGCG AAACTTGGCC AGTTACCTCT CACTGGCGGC CATCGATCAC 
GCCGGCTTGC TGAGCCCTCA TACGGAGTCC GTGATGGAGT CCATTCTGTC CGGAAACTAT 
GGTCTACTCC GAGTGCTTTC ACAGGTGTAC GAGGTGGCTC CGGAGGCGGT GACGCCACAT 
GCACCACTTC TCATGCCCCT GCTGCCACAG TGTGATCCGC AGGAGAGAAT GGCGGTGTTC 
CAGCTGTATC TGCTGATCGT TCAGAAGTCA CCAGAGGTTC TGGAATCTTG CGTTCCGCAA 
CTTTGTGGCT CCCTGCTCGA TAGCGACACT TCTAGCATAA CCATGCAGAT TCTGCTGAAG 
CTTTCCCAGC ACTCGCCCAG CCTTCTTGTC GACCATTTCG AGCAATTGCG CCTGGCAGCT 
AAAACCAATC CTGGAACGAT TCCGCTTTGT GCCCAGATTA TGACTACCGC GGGAATCGGG 
AGCAAGGAGA CGGCCCAGCA GGCTTTGGAC TTTGTGCTGG AGCACCTGCC CACGCAGGCA 
CTCCTGCAAC AGGAGGCCAC TCGCCTCTGT TCCGCCTATC CGGTTTTGTT CACCGACAAA 
GTACTGGCCT GCGTGCGGCA GAAGAACGCA GCTCTCAGCT CGCAACAGGA TGTGGGGAAT 
GGCTTGGCGA ACAAGACCTC TGGTGGTGTC ACCATTGTCA GCTTGAATAG CTCCAGTCCC 
AGTCCGCCGC TTAAGCCAGT TCCAGTTGGC GCACCAGCTA TTAACACCAC AATCGTCCAG 
CCGGCCAGCG TAGTCAGTGC CACGCCCACA CCACCTCCAT CCTCCGCCTC GGCGCCCATT 
GCCAGCTCCA CGCCCGTATC GGCTGCCACG CCCACTCCCC AGCATGCGGG ATACACGCGG 
CGGGTGAAGC TAGGCGATTC CCGGAGCACA GGTCGCCTGC ATCCCGCCAG CAATACGCAC 
CGGAGTGTGA CCCGGTTGAA TGTGGCCAGC GGATCCGTGG GAGGACTTCA CAAGAGCATG 
ACTCGGCTGA GTAACTCGCA GATTAACCAG CAACCTGGAG GCAGCTCCAA TGGGAATGTC 
GTGGTGCAAT CGGGGGCTAC TCCGAAGACC CCAACTGGTC TAAGCAATCC GCCTGTGACA 
CCAGTGCCGC CCTTGAGCAA CAACGTGGTC ATAACTGGAC ACAATCGACA TGGTATACCT 
GTTACCTCTG GAGGAGTGAC TGTGACCACG TCGCCCTCGA AGGTGCGACC ACACTCCCAA 
GGACCATCAA CGCTGCTCAA CTCGAGCACG GTGCTGATGA AGTACAGCAC GGATGCACTG 
AATCAATCGG TGGGCTCGAT CTCCATACCA CAGTCGGCAG CGGTGGCCAC TTTGCCATCG 
ACGCAGACAC AGAATGCGGT ATCCGTCCAT CATGCCTCGC CGGCGCTGCC CCAATCCGCC 
AGCAATGGTC ATGCCAAGTC AGTGATGAAA CTGCCAGTCA ACGGAAATAG CGAGGTAATC 
GTTTCGGGAC CCACCACCAA TGTGGCACCT CGGCGCAGCG ATAATACCAG CAGGACCCTA 
CTGAATGCCA ACAGTGTCAT GGACCAGCGG ATGAGCACCT TTGAGCCATA CCAGATTATG 
CGTGATCCTG TTCAGCAATT CTGCGAGAAG AACTTCAATT CGATCAAGTC CTACATGGAT 
GAGGTATCGC AGCATCTGCC GCCGCCCACA CGTTGCAGCA TTGAGGAGCG TCGTACCAAA 
AAGGTGGCCA AACTGCATTT CGCCTGCCAG ATTCGTGGTC CCCATTGCCT GTACTCCAAA 
ACTTGCTTCA CGATGCGAAC TCGGAACCCC AAGACCTGGA TACATATGAT GTTCCTGGAC 
TTTCAAGTGC GCCATTTTGT GAAGGAGAAG TGCGTGCTTA GCACGCGGGA GTCCGGCATT 
AGCAATCTCA AGAATATTTG GCAGATACTC AAGTGCGAGA ACCGCAGCTT CACCGAGCTG 
GTTACCAGTC AGTTTCCTCA GGTTAAGGAC CGCGAGATCC TGGTGAACGA GCTGCGTCAC 
TCGGGCTTTC TGGATGTGTT CGAGGTCTCC AAGACAGACA AGTCCAACCC AAACTGCAAT 
GAGTTGGAAT ACCAGTGGGG CTGCTTCCTC TGCAATCATC CGGACAAGGC GGTGGGCTTT 
CTCAATGGCA GCAATCAGCC CATGATCGAG GGCCAGCTGA AGGAGAAGAA GGGCAAGTGG 
CGACTATTCA GGCGCTGGCG AACGCGCTAC TTCACTTTGT CCGGAGCGCA CTTGTCCTGC 
AAAGGCTCTA GCGGAGGTGA GAGCATCGAT GTGAATCAGA TACGTTCGGT AAAGGTATCT 
CGTGGTGCCC GTAACATACC GAAGGCCTTC GAAATCTTCA CCGCCGACCA GACATTAATA 
CTCAAGCCCA AGGACGGCAA GAATGCCGAG GAGTGGGTGC AGTGCCTCAG CATCGTGGTA 
GCCCACTCGC AGGCCCGAGA TAATCCCACG GCCAAGACGA ACAGCCTGCC CGCCCGCAGC 
ATAGGCAGCA GCAAGCCCTC CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014572.1
CDS	763..3729
Translation	MHELFNKVLAKRDLSRAGDLFSVPDADIVDDITEVLSEISPIISHADYVKNNNDQSVVEICVTRVLSCIRETKTAERYCAALVDLLRTCLLWNLQPSGTTKEEPPHAKIAADIISSIFLNYDKKELMKIALPVAVQFLPKGNRELSRNLASYLSLAAIDHAGLLSPHTESVMESILSGNYGLLRVLSQVYEVAPEAVTPHAPLLMPLLPQCDPQERMAVFQLYLLIVQKSPEVLESCVPQLCGSLLDSDTSSITMQILLKLSQHSPSLLVDHFEQLRLAAKTNPGTIPLCAQIMTTAGIGSKETAQQALDFVLEHLPTQALLQQEATRLCSAYPVLFTDKVLACVRQKNAALSSQQDVGNGLANKTSGGVTIVSLNSSSPSPPLKPVPVGAPAINTTIVQPASVVSATPTPPPSSASAPIASSTPVSAATPTPQHAGYTRRVKLGDSRSTGRLHPASNTHRSVTRLNVASGSVGGLHKSMTRLSNSQINQQPGGSSNGNVVVQSGATPKTPTGLSNPPVTPVPPLSNNVVITGHNRHGIPVTSGGVTVTTSPSKVRPHSQGPSTLLNSSTVLMKYSTDALNQSVGSISIPQSAAVATLPSTQTQNAVSVHHASPALPQSASNGHAKSVMKLPVNGNSEVIVSGPTTNVAPRRSDNTSRTLLNANSVMDQRMSTFEPYQIMRDPVQQFCEKNFNSIKSYMDEVSQHLPPPTRCSIEERRTKKVAKLHFACQIRGPHCLYSKTCFTMRTRNPKTWIHMMFLDFQVRHFVKEKCVLSTRESGISNLKNIWQILKCENRSFTELVTSQFPQVKDREILVNELRHSGFLDVFEVSKTDKSNPNCNELEYQWGCFLCNHPDKAVGFLNGSNQPMIEGQLKEKKGKWRLFRRWRTRYFTLSGAHLSCKGSSGGESIDVNQIRSVKVSRGARNIPKAFEIFTADQTLILKPKDGKNAEEWVQCLSIVVAHSQARDNPTAKTNSLPARSIGSSKPSF

Target ORF information:

RefSeq Version	NM_001014572.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster melted (melt), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014572.4

ORF Insert Sequence:

ATGCACGAAC TTTTCAACAA AGTGCTCGCC AAACGCGATC TCTCAAGAGC CGGAGATCTC 
TTTTCCGTGC CGGATGCAGA TATAGTCGAC GACATCACGG AGGTGCTTTC CGAGATCAGT 
CCGATCATCT CGCACGCGGA CTATGTGAAG AACAACAATG ACCAGAGTGT GGTGGAGATC 
TGTGTGACAC GGGTGCTATC CTGCATCCGG GAAACGAAAA CTGCGGAGCG ATACTGTGCT 
GCTCTGGTGG ATCTTCTGAG GACCTGTCTG CTATGGAACC TCCAACCCTC GGGAACAACA 
AAGGAAGAGC CACCCCATGC CAAGATTGCT GCCGATATCA TCTCTAGCAT TTTCCTGAAC 
TATGACAAAA AAGAACTGAT GAAAATAGCC CTTCCCGTCG CCGTGCAGTT CCTTCCGAAG 
GGAAACCGTG AGCTATCGCG AAACTTGGCC AGTTACCTCT CACTGGCGGC CATCGATCAC 
GCCGGCTTGC TGAGCCCTCA TACGGAGTCC GTGATGGAGT CCATTCTGTC CGGAAACTAT 
GGTCTACTCC GAGTGCTTTC ACAGGTGTAC GAGGTGGCTC CGGAGGCGGT GACGCCACAT 
GCACCACTTC TCATGCCCCT GCTGCCACAG TGTGATCCGC AGGAGAGAAT GGCGGTGTTC 
CAGCTGTATC TGCTGATCGT TCAGAAGTCA CCAGAGGTTC TGGAATCTTG CGTTCCGCAA 
CTTTGTGGCT CCCTGCTCGA TAGCGACACT TCTAGCATAA CCATGCAGAT TCTGCTGAAG 
CTTTCCCAGC ACTCGCCCAG CCTTCTTGTC GACCATTTCG AGCAATTGCG CCTGGCAGCT 
AAAACCAATC CTGGAACGAT TCCGCTTTGT GCCCAGATTA TGACTACCGC GGGAATCGGG 
AGCAAGGAGA CGGCCCAGCA GGCTTTGGAC TTTGTGCTGG AGCACCTGCC CACGCAGGCA 
CTCCTGCAAC AGGAGGCCAC TCGCCTCTGT TCCGCCTATC CGGTTTTGTT CACCGACAAA 
GTACTGGCCT GCGTGCGGCA GAAGAACGCA GCTCTCAGCT CGCAACAGGA TGTGGGGAAT 
GGCTTGGCGA ACAAGACCTC TGGTGGTGTC ACCATTGTCA GCTTGAATAG CTCCAGTCCC 
AGTCCGCCGC TTAAGCCAGT TCCAGTTGGC GCACCAGCTA TTAACACCAC AATCGTCCAG 
CCGGCCAGCG TAGTCAGTGC CACGCCCACA CCACCTCCAT CCTCCGCCTC GGCGCCCATT 
GCCAGCTCCA CGCCCGTATC GGCTGCCACG CCCACTCCCC AGCATGCGGG ATACACGCGG 
CGGGTGAAGC TAGGCGATTC CCGGAGCACA GGTCGCCTGC ATCCCGCCAG CAATACGCAC 
CGGAGTGTGA CCCGGTTGAA TGTGGCCAGC GGATCCGTGG GAGGACTTCA CAAGAGCATG 
ACTCGGCTGA GTAACTCGCA GATTAACCAG CAACCTGGAG GCAGCTCCAA TGGGAATGTC 
GTGGTGCAAT CGGGGGCTAC TCCGAAGACC CCAACTGGTC TAAGCAATCC GCCTGTGACA 
CCAGTGCCGC CCTTGAGCAA CAACGTGGTC ATAACTGGAC ACAATCGACA TGGTATACCT 
GTTACCTCTG GAGGAGTGAC TGTGACCACG TCGCCCTCGA AGGTGCGACC ACACTCCCAA 
GGACCATCAA CGCTGCTCAA CTCGAGCACG GTGCTGATGA AGTACAGCAC GGATGCACTG 
AATCAATCGG TGGGCTCGAT CTCCATACCA CAGTCGGCAG CGGTGGCCAC TTTGCCATCG 
ACGCAGACAC AGAATGCGGT ATCCGTCCAT CATGCCTCGC CGGCGCTGCC CCAATCCGCC 
AGCAATGGTC ATGCCAAGTC AGTGATGAAA CTGCCAGTCA ACGGAAATAG CGAGGTAATC 
GTTTCGGGAC CCACCACCAA TGTGGCACCT CGGCGCAGCG ATAATACCAG CAGGACCCTA 
CTGAATGCCA ACAGTGTCAT GGACCAGCGG ATGAGCACCT TTGAGCCATA CCAGATTATG 
CGTGATCCTG TTCAGCAATT CTGCGAGAAG AACTTCAATT CGATCAAGTC CTACATGGAT 
GAGGTATCGC AGCATCTGCC GCCGCCCACA CGTTGCAGCA TTGAGGAGCG TCGTACCAAA 
AAGGTGGCCA AACTGCATTT CGCCTGCCAG ATTCGTGGTC CCCATTGCCT GTACTCCAAA 
ACTTGCTTCA CGATGCGAAC TCGGAACCCC AAGACCTGGA TACATATGAT GTTCCTGGAC 
TTTCAAGTGC GCCATTTTGT GAAGGAGAAG TGCGTGCTTA GCACGCGGGA GTCCGGCATT 
AGCAATCTCA AGAATATTTG GCAGATACTC AAGTGCGAGA ACCGCAGCTT CACCGAGCTG 
GTTACCAGTC AGTTTCCTCA GGTTAAGGAC CGCGAGATCC TGGTGAACGA GCTGCGTCAC 
TCGGGCTTTC TGGATGTGTT CGAGGTCTCC AAGACAGACA AGTCCAACCC AAACTGCAAT 
GAGTTGGAAT ACCAGTGGGG CTGCTTCCTC TGCAATCATC CGGACAAGGC GGTGGGCTTT 
CTCAATGGCA GCAATCAGCC CATGATCGAG GGCCAGCTGA AGGAGAAGAA GGGCAAGTGG 
CGACTATTCA GGCGCTGGCG AACGCGCTAC TTCACTTTGT CCGGAGCGCA CTTGTCCTGC 
AAAGGCTCTA GCGGAGGTGA GAGCATCGAT GTGAATCAGA TACGTTCGGT AAAGGTATCT 
CGTGGTGCCC GTAACATACC GAAGGCCTTC GAAATCTTCA CCGCCGACCA GACATTAATA 
CTCAAGCCCA AGGACGGCAA GAATGCCGAG GAGTGGGTGC AGTGCCTCAG CATCGTGGTA 
GCCCACTCGC AGGCCCGAGA TAATCCCACG GCCAAGACGA ACAGCCTGCC CGCCCGCAGC 
ATAGGCAGCA GCAAGCCCTC CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

melt ( NM_001274566.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261495.1 Drosophila melanogaster melted (melt), transcript variant D, mRNA.

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CloneID	OFa20624
Clone ID Related Accession (Same CDS sequence)	NM_001274566.1
Accession Version	NM_001274566.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	melted, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCACGAAC TTTTCAACAA AGTGCTCGCC AAACGCGATC TCTCAAGAGC CGGAGATCTC 
TTTTCCGTGC CGGATGCAGA TATAGTCGAC GACATCACGG AGGTGCTTTC CGAGATCAGT 
CCGATCATCT CGCACGCGGA CTATGTGAAG AACAACAATG ACCAGAGTGT GGTGGAGATC 
TGTGTGACAC GGGTGCTATC CTGCATCCGG GAAACGAAAA CTGCGGAGCG ATACTGTGCT 
GCTCTGGTGG ATCTTCTGAG GACCTGTCTG CTATGGAACC TCCAACCCTC GGGAACAACA 
AAGGAAGAGC CACCCCATGC CAAGATTGCT GCCGATATCA TCTCTAGCAT TTTCCTGAAC 
TATGACAAAA AAGAACTGAT GAAAATAGCC CTTCCCGTCG CCGTGCAGTT CCTTCCGAAG 
GGAAACCGTG AGCTATCGCG AAACTTGGCC AGTTACCTCT CACTGGCGGC CATCGATCAC 
GCCGGCTTGC TGAGCCCTCA TACGGAGTCC GTGATGGAGT CCATTCTGTC CGGAAACTAT 
GGTCTACTCC GAGTGCTTTC ACAGGTGTAC GAGGTGGCTC CGGAGGCGGT GACGCCACAT 
GCACCACTTC TCATGCCCCT GCTGCCACAG TGTGATCCGC AGGAGAGAAT GGCGGTGTTC 
CAGCTGTATC TGCTGATCGT TCAGAAGTCA CCAGAGGTTC TGGAATCTTG CGTTCCGCAA 
CTTTGTGGCT CCCTGCTCGA TAGCGACACT TCTAGCATAA CCATGCAGAT TCTGCTGAAG 
CTTTCCCAGC ACTCGCCCAG CCTTCTTGTC GACCATTTCG AGCAATTGCG CCTGGCAGCT 
AAAACCAATC CTGGAACGAT TCCGCTTTGT GCCCAGATTA TGACTACCGC GGGAATCGGG 
AGCAAGGAGA CGGCCCAGCA GGCTTTGGAC TTTGTGCTGG AGCACCTGCC CACGCAGGCA 
CTCCTGCAAC AGGAGGCCAC TCGCCTCTGT TCCGCCTATC CGGTTTTGTT CACCGACAAA 
GTACTGGCCT GCGTGCGGCA GAAGAACGCA GCTCTCAGCT CGCAACAGGA TGTGGGGAAT 
GGCTTGGCGA ACAAGACCTC TGGTGGTGTC ACCATTGTCA GCTTGAATAG CTCCAGTCCC 
AGTCCGCCGC TTAAGCCAGT TGGCGCACCA GCTATTAACA CCACAATCGT CCAGCCGGCC 
AGCGTAGTCA GTGCCACGCC CACACCACCT CCATCCTCCG CCTCGGCGCC CATTGCCAGC 
TCCACGCCCG TATCGGCTGC CACGCCCACT CCCCAGCATG CGGGATACAC GCGGCGGGTG 
AAGCTAGGCG ATTCCCGGAG CACAGGTCGC CTGCATCCCG CCAGCAATAC GCACCGGAGT 
GTGACCCGGT TGAATGTGGC CAGCGGATCC GTGGGAGGAC TTCACAAGAG CATGACTCGG 
CTGAGTAACT CGCAGATTAA CCAGCAACCT GGAGGCAGCT CCAATGGGAA TGTCGTGGTG 
CAATCGGGGG CTACTCCGAA GACCCCAACT GGTCTAAGCA ATCCGCCTGT GACACCAGTG 
CCGCCCTTGA GCAACAACGT GGTCATAACT GGACACAATC GACATGGTAT ACCTGTTACC 
TCTGGAGGAG TGACTGTGAC CACGTCGCCC TCGAAGGTGC GACCACACTC CCAAGGACCA 
TCAACGCTGC TCAACTCGAG CACGGTGCTG ATGAAGTACA GCACGGATGC ACTGAATCAA 
TCGGTGGGCT CGATCTCCAT ACCACAGTCG GCAGCGGTGG CCACTTTGCC ATCGACGCAG 
ACACAGAATG CGGTATCCGT CCATCATGCC TCGCCGGCGC TGCCCCAATC CGCCAGCAAT 
GGTCATGCCA AGTCAGTGAT GAAACTGCCA GTCAACGGAA ATAGCGAGGT AATCGTTTCG 
GGACCCACCA CCAATGTGGC ACCTCGGCGC AGCGATAATA CCAGCAGGAC CCTACTGAAT 
GCCAACAGTG TCATGGACCA GCGGATGAGC ACCTTTGAGC CATACCAGAT TATGCGTGAT 
CCTGTTCAGC AATTCTGCGA GAAGAACTTC AATTCGATCA AGTCCTACAT GGATGAGGTA 
TCGCAGCATC TGCCGCCGCC CACACGTTGC AGCATTGAGG TTTCCAATGA ATTTGCAGAG 
CGTCGTACCA AAAAGGTGGC CAAACTGCAT TTCGCCTGCC AGATTCGTGG TCCCCATTGC 
CTGTACTCCA AAACTTGCTT CACGATGCGA ACTCGGAACC CCAAGACCTG GATACATATG 
ATGTTCCTGG ACTTTCAAGT GCGCCATTTT GTGAAGGAGA AGTGCGTGCT TAGCACGCGG 
GAGTCCGGCA TTAGCAATCT CAAGAATATT TGGCAGATAC TCAAGTGCGA GAACCGCAGC 
TTCACCGAGC TGGTTACCAG TCAGTTTCCT CAGGTTAAGG ACCGCGAGAT CCTGGTGAAC 
GAGCTGCGTC ACTCGGGCTT TCTGGATGTG TTCGAGGTCT CCAAGACAGA CAAGTCCAAC 
CCAAACTGCA ATGAGTTGGA ATACCAGTGG GGCTGCTTCC TCTGCAATCA TCCGGACAAG 
GCGGTGGGCT TTCTCAATGG CAGCAATCAG CCCATGATCG AGGGCCAGCT GAAGGAGAAG 
AAGGGCAAGT GGCGACTATT CAGGCGCTGG CGAACGCGCT ACTTCACTTT GTCCGGAGCG 
CACTTGTCCT GCAAAGGCTC TAGCGGAGGT GAGAGCATCG ATGTGAATCA GATACGTTCG 
GTAAAGGTAT CTCGTGGTGC CCGTAACATA CCGAAGGCCT TCGAAATCTT CACCGCCGAC 
CAGACATTAA TACTCAAGCC CAAGGACGGC AAGAATGCCG AGGAGTGGGT GCAGTGCCTC 
AGCATCGTGG TAGCCCACTC GCAGGCCCGA GATAATCCCA CGGCCAAGAC GAACAGCCTG 
CCCGCCCGCA GCATAGGCAG CAGCAAGCCC TCCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261495.1
CDS	1049..4027
Translation	MHELFNKVLAKRDLSRAGDLFSVPDADIVDDITEVLSEISPIISHADYVKNNNDQSVVEICVTRVLSCIRETKTAERYCAALVDLLRTCLLWNLQPSGTTKEEPPHAKIAADIISSIFLNYDKKELMKIALPVAVQFLPKGNRELSRNLASYLSLAAIDHAGLLSPHTESVMESILSGNYGLLRVLSQVYEVAPEAVTPHAPLLMPLLPQCDPQERMAVFQLYLLIVQKSPEVLESCVPQLCGSLLDSDTSSITMQILLKLSQHSPSLLVDHFEQLRLAAKTNPGTIPLCAQIMTTAGIGSKETAQQALDFVLEHLPTQALLQQEATRLCSAYPVLFTDKVLACVRQKNAALSSQQDVGNGLANKTSGGVTIVSLNSSSPSPPLKPVGAPAINTTIVQPASVVSATPTPPPSSASAPIASSTPVSAATPTPQHAGYTRRVKLGDSRSTGRLHPASNTHRSVTRLNVASGSVGGLHKSMTRLSNSQINQQPGGSSNGNVVVQSGATPKTPTGLSNPPVTPVPPLSNNVVITGHNRHGIPVTSGGVTVTTSPSKVRPHSQGPSTLLNSSTVLMKYSTDALNQSVGSISIPQSAAVATLPSTQTQNAVSVHHASPALPQSASNGHAKSVMKLPVNGNSEVIVSGPTTNVAPRRSDNTSRTLLNANSVMDQRMSTFEPYQIMRDPVQQFCEKNFNSIKSYMDEVSQHLPPPTRCSIEVSNEFAERRTKKVAKLHFACQIRGPHCLYSKTCFTMRTRNPKTWIHMMFLDFQVRHFVKEKCVLSTRESGISNLKNIWQILKCENRSFTELVTSQFPQVKDREILVNELRHSGFLDVFEVSKTDKSNPNCNELEYQWGCFLCNHPDKAVGFLNGSNQPMIEGQLKEKKGKWRLFRRWRTRYFTLSGAHLSCKGSSGGESIDVNQIRSVKVSRGARNIPKAFEIFTADQTLILKPKDGKNAEEWVQCLSIVVAHSQARDNPTAKTNSLPARSIGSSKPSF

Target ORF information:

RefSeq Version	NM_001274566.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster melted (melt), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274566.1

ORF Insert Sequence:

ATGCACGAAC TTTTCAACAA AGTGCTCGCC AAACGCGATC TCTCAAGAGC CGGAGATCTC 
TTTTCCGTGC CGGATGCAGA TATAGTCGAC GACATCACGG AGGTGCTTTC CGAGATCAGT 
CCGATCATCT CGCACGCGGA CTATGTGAAG AACAACAATG ACCAGAGTGT GGTGGAGATC 
TGTGTGACAC GGGTGCTATC CTGCATCCGG GAAACGAAAA CTGCGGAGCG ATACTGTGCT 
GCTCTGGTGG ATCTTCTGAG GACCTGTCTG CTATGGAACC TCCAACCCTC GGGAACAACA 
AAGGAAGAGC CACCCCATGC CAAGATTGCT GCCGATATCA TCTCTAGCAT TTTCCTGAAC 
TATGACAAAA AAGAACTGAT GAAAATAGCC CTTCCCGTCG CCGTGCAGTT CCTTCCGAAG 
GGAAACCGTG AGCTATCGCG AAACTTGGCC AGTTACCTCT CACTGGCGGC CATCGATCAC 
GCCGGCTTGC TGAGCCCTCA TACGGAGTCC GTGATGGAGT CCATTCTGTC CGGAAACTAT 
GGTCTACTCC GAGTGCTTTC ACAGGTGTAC GAGGTGGCTC CGGAGGCGGT GACGCCACAT 
GCACCACTTC TCATGCCCCT GCTGCCACAG TGTGATCCGC AGGAGAGAAT GGCGGTGTTC 
CAGCTGTATC TGCTGATCGT TCAGAAGTCA CCAGAGGTTC TGGAATCTTG CGTTCCGCAA 
CTTTGTGGCT CCCTGCTCGA TAGCGACACT TCTAGCATAA CCATGCAGAT TCTGCTGAAG 
CTTTCCCAGC ACTCGCCCAG CCTTCTTGTC GACCATTTCG AGCAATTGCG CCTGGCAGCT 
AAAACCAATC CTGGAACGAT TCCGCTTTGT GCCCAGATTA TGACTACCGC GGGAATCGGG 
AGCAAGGAGA CGGCCCAGCA GGCTTTGGAC TTTGTGCTGG AGCACCTGCC CACGCAGGCA 
CTCCTGCAAC AGGAGGCCAC TCGCCTCTGT TCCGCCTATC CGGTTTTGTT CACCGACAAA 
GTACTGGCCT GCGTGCGGCA GAAGAACGCA GCTCTCAGCT CGCAACAGGA TGTGGGGAAT 
GGCTTGGCGA ACAAGACCTC TGGTGGTGTC ACCATTGTCA GCTTGAATAG CTCCAGTCCC 
AGTCCGCCGC TTAAGCCAGT TGGCGCACCA GCTATTAACA CCACAATCGT CCAGCCGGCC 
AGCGTAGTCA GTGCCACGCC CACACCACCT CCATCCTCCG CCTCGGCGCC CATTGCCAGC 
TCCACGCCCG TATCGGCTGC CACGCCCACT CCCCAGCATG CGGGATACAC GCGGCGGGTG 
AAGCTAGGCG ATTCCCGGAG CACAGGTCGC CTGCATCCCG CCAGCAATAC GCACCGGAGT 
GTGACCCGGT TGAATGTGGC CAGCGGATCC GTGGGAGGAC TTCACAAGAG CATGACTCGG 
CTGAGTAACT CGCAGATTAA CCAGCAACCT GGAGGCAGCT CCAATGGGAA TGTCGTGGTG 
CAATCGGGGG CTACTCCGAA GACCCCAACT GGTCTAAGCA ATCCGCCTGT GACACCAGTG 
CCGCCCTTGA GCAACAACGT GGTCATAACT GGACACAATC GACATGGTAT ACCTGTTACC 
TCTGGAGGAG TGACTGTGAC CACGTCGCCC TCGAAGGTGC GACCACACTC CCAAGGACCA 
TCAACGCTGC TCAACTCGAG CACGGTGCTG ATGAAGTACA GCACGGATGC ACTGAATCAA 
TCGGTGGGCT CGATCTCCAT ACCACAGTCG GCAGCGGTGG CCACTTTGCC ATCGACGCAG 
ACACAGAATG CGGTATCCGT CCATCATGCC TCGCCGGCGC TGCCCCAATC CGCCAGCAAT 
GGTCATGCCA AGTCAGTGAT GAAACTGCCA GTCAACGGAA ATAGCGAGGT AATCGTTTCG 
GGACCCACCA CCAATGTGGC ACCTCGGCGC AGCGATAATA CCAGCAGGAC CCTACTGAAT 
GCCAACAGTG TCATGGACCA GCGGATGAGC ACCTTTGAGC CATACCAGAT TATGCGTGAT 
CCTGTTCAGC AATTCTGCGA GAAGAACTTC AATTCGATCA AGTCCTACAT GGATGAGGTA 
TCGCAGCATC TGCCGCCGCC CACACGTTGC AGCATTGAGG TTTCCAATGA ATTTGCAGAG 
CGTCGTACCA AAAAGGTGGC CAAACTGCAT TTCGCCTGCC AGATTCGTGG TCCCCATTGC 
CTGTACTCCA AAACTTGCTT CACGATGCGA ACTCGGAACC CCAAGACCTG GATACATATG 
ATGTTCCTGG ACTTTCAAGT GCGCCATTTT GTGAAGGAGA AGTGCGTGCT TAGCACGCGG 
GAGTCCGGCA TTAGCAATCT CAAGAATATT TGGCAGATAC TCAAGTGCGA GAACCGCAGC 
TTCACCGAGC TGGTTACCAG TCAGTTTCCT CAGGTTAAGG ACCGCGAGAT CCTGGTGAAC 
GAGCTGCGTC ACTCGGGCTT TCTGGATGTG TTCGAGGTCT CCAAGACAGA CAAGTCCAAC 
CCAAACTGCA ATGAGTTGGA ATACCAGTGG GGCTGCTTCC TCTGCAATCA TCCGGACAAG 
GCGGTGGGCT TTCTCAATGG CAGCAATCAG CCCATGATCG AGGGCCAGCT GAAGGAGAAG 
AAGGGCAAGT GGCGACTATT CAGGCGCTGG CGAACGCGCT ACTTCACTTT GTCCGGAGCG 
CACTTGTCCT GCAAAGGCTC TAGCGGAGGT GAGAGCATCG ATGTGAATCA GATACGTTCG 
GTAAAGGTAT CTCGTGGTGC CCGTAACATA CCGAAGGCCT TCGAAATCTT CACCGCCGAC 
CAGACATTAA TACTCAAGCC CAAGGACGGC AAGAATGCCG AGGAGTGGGT GCAGTGCCTC 
AGCATCGTGG TAGCCCACTC GCAGGCCCGA GATAATCCCA CGGCCAAGAC GAACAGCCTG 
CCCGCCCGCA GCATAGGCAG CAGCAAGCCC TCCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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melt cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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